JP2003325148A - Healthy drink - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a healthy drink in which oxidation on a gene of a first component which contains white birch sap as an active ingredient is reduced by adding a second component which reduces the oxidation on the gene of the first component. <P>SOLUTION: This healthy drink contains the first component which contains the white birch sap as the active ingredient and the second component which reduces the oxidation on the gene of the first component, wherein the second component comprises a water-soluble nucleoprotein. Anti-oxidizing water which is obtained by treating water having a nitrogen oxide content of ≤0.1 ppm with a semiconductor catalyst in a magnetic field is used as the second component in the drink. Further, the water-soluble nucleoprotein and/or the anti- oxidizing water is used as the second component in the drink. The healthy drink contains the active ingredient, scarcely has the oxidation on the gene, and is useful for maintenance/promotion of the human health. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a health drink, and more specifically, to the gene-oxidizing action of the first component containing white birch sap as an active ingredient, and the second component as a water-soluble nuclear protein and / or nitrogen oxide. The present invention relates to a health drink in which water having a concentration of 0.1 ppm or less is reduced by adding antioxidant water treated with a semiconductor catalyst in a magnetic field.

[0002]

2. Description of the Related Art In recent years, various drinks and foods effective for maintaining health, so-called health drinks and health foods have been marketed, reflecting the growing public interest in health. Air and water are typical examples of substances that animals including humans need to maintain their health properly. Therefore, there is a particular interest in water in the field of health drinks and foods.

Typical examples of healthy drinks are so-called stamina drinks and sports drinks. Stamina drinks and sports drinks are generally manufactured artificially by adding active ingredients such as vitamins, amino acids, minerals and sugars to pure water.

By the way, a wide variety of animals that live in nature ingest the sap of various trees in order to maintain their lives. Sap is a so-called natural drink that is rich in various components essential for maintaining the life and health of animals. There are a huge variety of trees on Earth, and the sap may contain unknown components useful for maintaining human life and health. Therefore, a healthy beverage containing sap as an active ingredient is very promising because it can be a characteristic healthy beverage unlike an artificially produced healthy beverage in which the ingredients tend to be uniform.

[0005] Birch sap is one of the above natural drinks, has a gastrointestinal effect, for example, a preventive effect on gastric ulcer, and is useful for maintaining / promoting human health. Below, it is already on the market from Supp.

Oxidation of genes induces deterioration and damage of genes. Reflecting growing public interest in environmental assessment, eliminating or suppressing as much as possible the oxidative effect on genes from the living environment is considered to be extremely important for maintaining and promoting human health. became.

The harmful effects of oxidative action on genes will be described below. The genetic information of an organism is such that adenine, thymine,
It is no longer known that it is encoded by the four bases of guanine and cytosine. However, genes can be oxidatively damaged at a certain frequency due to ultraviolet irradiation, exposure to various chemical substances, especially carcinogens and reactive oxygen species, and replication errors can occur. This replication error causes deterioration of genetic information and causes various diseases and death of cells and individuals. Therefore, by examining whether a substance promotes or suppresses the oxidative damage of a gene, it is possible to judge whether the substance has a toxicity or an effect on an organism. .

[0008] As a biological evaluation method for measuring the damage intensity of a substance causing gene damage, WO016923
The one described in Japanese Patent No. 5 is cited. In this method, attention is paid to the oxidation of mononucleosides constituting a gene, and 2'-deoxyguanosine (d) is added to a solution containing a test substance.
G) was added, and after an elapse of a certain period of time and / or an oxidative load such as UV irradiation, an oxidant of dG present in the solution: 8-hydroxy-2′-deoxyguanosine (8
The concentration of OHdG) is measured. And the generated 8OH
Based on the amount of dG, the presence or absence and the degree of toxicity or effectiveness of the test substance are determined. The higher the amount of 8OHdG produced, the higher the toxicity of the test substance.
The smaller the amount of G produced, the higher the effectiveness of the test substance.

[0009]

A recent study based on the above-mentioned biological evaluation method for measuring the production amount of 8OHdG has surprisingly revealed that birch sap has an oxidative effect on genes. Therefore, it is required to reduce the oxidative effect of birch sap on genes, and if this is possible, an even more excellent health drink can be provided. However, it is not preferable to use a means for impairing the flavor of the white birch sap as a natural drink in order to reduce the oxidative effect on the gene.

The present invention is intended to solve the above-mentioned problems of the prior art, and an object thereof is to include birch sap as an active ingredient and a component for reducing the oxidative action of the birch sap on genes. The purpose is to provide a healthy beverage containing

[0011]

[Means for Solving the Problems] That is, the health drink of the first invention comprises a first component containing birch sap as an active ingredient and a second component for reducing the oxidative action of the first component on a gene. The second component is a water-soluble nucleoprotein (hereinafter referred to as i). The health drink of the first aspect of the present invention preferably has the following aspects. ii) The health drink of i, wherein the water-soluble nucleoprotein is a nucleoprotein solubilized by lowering the molecular weight of the salmon, trout or herring albino nucleoprotein. iii) The water-soluble nucleoprotein comprises a low molecular weight oligonucleotide / nucleoside obtained by treating nucleolar proteins in algae with a nuclease and a protease and an oligopeptide in an amount of 20% by mass or more. Beverages. The health beverage of the second invention of the present invention comprises a first component containing white birch sap as an active ingredient, and a second component for reducing the oxidizing action of the first component on a gene, and the second component is
It is characterized in that it is antioxidant water obtained by treating water having a nitrogen oxide concentration of 0.1 ppm or less with a semiconductor catalyst in a magnetic field (hereinafter referred to as iv). The health drink of the second invention of the present invention preferably has the following aspects. v) iv health drink, wherein the semiconductor catalyst is an oxide semiconductor catalyst. vi) A health drink according to v, wherein the oxide semiconductor catalyst is titanium dioxide. The health beverage of the third invention comprises a first component containing white birch sap as an active ingredient, and a second component for reducing the oxidative action of the first component on a gene, and the second component is a water-soluble nuclear protein. And / or nitrogen oxide concentration 0.1 pp
It is characterized in that it is antioxidant water obtained by treating water of m or less with a semiconductor catalyst in a magnetic field.

[0012]

BEST MODE FOR CARRYING OUT THE INVENTION There are no particular limitations on the type of birch and the vegetation used for collecting the birch sap, which is the active ingredient of the first component, and the types of birch produced in Japan or foreign countries and the vegetation thereof can be used. Good. The white birch sap may be freshly collected, or may be stored by an appropriate method (for example, frozen / refrigerated storage, concentrated frozen / refrigerated storage, dry frozen / refrigerated storage, etc.) as appropriate. Good. The nutritional components of white birch sap are illustrated in Table 1 below. If necessary, other commonly used additive components such as flavors and colorants may be added to the first component in appropriate amounts. As the first component, a commercially available product (for example, a trade name "Mori no Shizuku" manufactured by Supp Co., Ltd.) can be used as it is.

The second component is a water-soluble nucleoprotein (the first invention of the present invention), or antioxidant water obtained by treating water having a nitrogen oxide concentration of 0.1 ppm or less with a semiconductor catalyst in a magnetic field (the second invention of the present invention), or The water-soluble nucleoprotein and / or the antioxidant water (the third invention of the present invention). The second component reduces by directly inhibiting or suppressing the oxidative action of the first component, or has a reducing action on the gene as opposed to the first component, and as a result , It is considered that the oxidative action of the first component on the gene is reduced.

Water-soluble nucleoprotein is the second component of the health drink of the first invention. The water-soluble nucleoprotein can be produced from Miko (testis of fish). Shirako is an underutilized marine resource that is currently only partially used for food and mostly discarded.
However, it has been found that algae contains a large amount of nucleic acid containing DNA as a main component and protein containing protamine as a main component, and has an effect of significantly reducing the oxidative action of the first component on a gene.

In the water-soluble nuclear protein, the molecular weight of the nuclear protein is reduced. The molecular weight reduction is preferably carried out to the extent that the oligonucleotide / nucleoside and oligopeptide are contained in the water-soluble nuclear protein. By reducing the molecular weight of the nucleic acid and protein in the cell nucleus in this way, digestion in vivo can be facilitated while maintaining the original effectiveness. Since the low molecular weight nuclear protein becomes water-soluble, it can be easily incorporated into a health drink. The water-solubility referred to here is a property of being soluble in water at a concentration of 0.1% by mass or more.

The water-soluble nucleoprotein thus obtained is
It has a good inhibitory effect on gene oxidative damage. Gene oxidative damage causes deterioration of genetic information encoded in the gene and causes various diseases and death, but a water-soluble nucleoprotein causes oxidative damage when an oxidative load is applied to a gene. Has the effect of significantly suppressing

The usefulness of the water-soluble nucleoprotein will be described in more detail below. It is known that the cell nucleus of fish contains a large amount of protamine, which is a protein containing arginine as a main component, and an oligopeptide obtained by degrading protamine exhibits excellent effects such as antiallergic action and skin beautifying action. Nucleic acid has the effect of preventing and improving beautiful skin and lifestyle-related diseases. Therefore, by producing a low molecular weight nucleoprotein (water-soluble nucleoprotein) using shirako as a raw material, it is possible to obtain a product having an excellent effect of suppressing gene oxidative damage and also having an effect derived from nucleic acid and protamine. . Examples of the fish include salmon, trout, herring, cod, and the like, and salmon, trout, and herring are particularly preferable. A water-soluble nuclear protein can be produced by removing skins, muscles, blood vessels and the like from these algae, purifying them to remove oil, and subjecting them to enzymatic decomposition treatment with nuclease and protease.

The water-soluble nucleoprotein may be added to the present health drink in the form of an aqueous solution, or may be once dried and solidified into a dosage form such as tablets, granules and capsules, and then returned to the form of an aqueous solution at the time of use. It is also possible to use. Since the water-soluble nuclear protein has a low molecular weight, it is easily digested. The intake amount of the water-soluble nuclear protein is preferably, for example, 50 to 5000 mg / day in terms of nucleic acid. The amount of water-soluble nucleoprotein added to this health drink is
It is appropriately selected in consideration of the intake amount. For example, assuming that one healthy drink is consumed per day, 50 to 5000 mg of water-soluble nuclear protein is converted into nucleic acid per one healthy drink.
Good to add. The water-soluble nucleoprotein used in this health drink is a low-molecular-weight oligonucleotide obtained by treating nucleoprotein in algae with nuclease and protease.
It is preferable that the nucleoside and the oligopeptide are contained in an amount of 20% by mass or more.

Antioxidant water obtained by treating water having a nitrogen oxide concentration of 0.1 ppm or less with a semiconductor catalyst in a magnetic field is the second component of the health drink of the second invention. Nitrogen oxide concentration 0.1
When water of ppm level or less, for example, deep groundwater, for example, pure water taken from a deep well at the foot of Takayama, is treated with a semiconductor catalyst in a magnetic field, trace harmful impurities in water, such as nitrogen oxides, sulfur oxides, ozone, and unpleasant odor The components are charged into charged particles and decomposed and removed by the semiconductor catalyst,
It is known to obtain antioxidant water that can reduce oxidative damage of genes. As such water, for example, water manufactured by Supreme Co., Ltd. is sold under the trade name "Supreme Water", and this can be used as the second component of the health drink of the present invention.

Examples of the semiconductor catalyst include TiO 2.
₂ , ZnO, Nb ₂ O ₅ , SrTiO ₃ , PbNb ₂ O
Examples thereof include metal oxides such as ₆ , K ₄ Nb ₆ O ₁₇ , metal sulfides such as CdS and ZnS, and organic polymers such as polyparaphenylene, which can be appropriately selected. Among the above semiconductor catalysts, oxide semiconductor catalysts are preferable, and titanium dioxide (TiO ₂ ) which causes an oxidation / reduction reaction is most preferable.

The strength of the magnetic field in the magnetic field catalytic treatment of water having a nitrogen oxide concentration of 0.1 ppm or less can be appropriately selected, but is preferably 0.1 Tesla (1000 gauss) or more. Further, it is more effective to apply kinetic energy by a method suitable for water containing the charged particles during the magnetic field catalytic treatment. The method of applying kinetic energy to the water to be treated containing charged particles includes, for example, a method of linearly or rotationally moving the water to be treated, a method of applying ultrasonic waves or microwaves to the water to be treated, and the semiconductor catalyst. There is a method of indirectly imparting kinetic energy to the water to be treated by imparting kinetic energy to the side of.

The strength of the kinetic energy applied to the water to be treated can be appropriately selected. If necessary, the semiconductor catalyst may be used in combination with another catalyst, and other processing conditions such as pH may be appropriately selected.

The health drink according to the third aspect of the present invention uses the water-soluble nucleoprotein and / or the antioxidant water as the second component. When water-soluble nucleoprotein and antioxidant water are used in combination, the mixing ratio of water-soluble nucleoprotein and antioxidant water can be appropriately selected. The water-soluble nucleoprotein and the antioxidant water may be premixed in a predetermined ratio, or may be separately added to the first component.

In addition to the first component and the second component,
Further enhance the effect of the first component and the second component,
Alternatively, in addition to the effects of the first component and the second component, a third component that imparts another effect may be further added.
Specific examples of the third component are, for example, various vitamins, minerals, sugars and the like.

A mixing ratio of the first component and the second component,
Alternatively, the mixing ratio of the first component, the second component, and the third component can be appropriately selected within a range that does not impair the effects of the health drink of the present invention.

[0026]

The present invention will be described in more detail by the following examples. <Evaluation of oxidative action on gene> The oxidative action of a test substance on a gene is evaluated by measuring the ratio of a gene in a biological sample (blood or urine) between an oxidatively damaged gene (8OHdG) and a normal gene (dG). It went by investigating. The procedure of a gene oxidative damage test (efficacy evaluation test) will be described below. <Procedure for gene oxidative damage test>2'- with a concentration of 200 μg / ml using ultrapure water
A deoxyguanosine standard solution (dG standard solution, manufactured by Wako Pure Chemical Industries, Ltd.) is prepared. 2. Dilute the dG standard solution 10 times with the test solution,
Use as a μg / ml dG solution (test solution). 3. The test solution is placed in a dark box, and exposed to 254 nm ultraviolet light (800 μW / cm ² , a dose of about 10 times that in direct sunlight in the mid-latitude zone of the northern hemisphere) that induces oxidative damage of genes. 4. The test solution is sampled over time, and the concentrations of 8-hydroxy-2′-deoxyguanosine (8OHdG) ^{* 1} and dG in the solution are measured. The concentration of 8OHdG and dG was measured by high performance liquid chromatography (HPL) of the test solution.
It was carried out by detecting with an electrochemical detector (ECD). 5. When the test solution has a strong oxidative inducibility (oxidative damage) to a gene, a phenomenon is observed in which the induced 8OHdG is further oxidized and the apparent 8OHdG content becomes low. In such cases, add 10% to the test solution at each concentration.
A standard 8OHdG solution of μg / ml was added, and the same operation was performed to measure the reduction rate of the 8OHdG concentration. The measured value of 8OHdG of the test solution was multiplied by the reciprocal of the reduction rate, The value of 8OHdG induced by oxidation is corrected. 6. The degree of oxidative damage of each test solution was measured as “8OHdG / d
G "or" 8OHdG correction value / dG ". ^{* 1} : 8-hydroxy-2'-deoxyguanosine (8O
HdG) is a compound in which a hydroxyl group is bonded to the 8-position carbon of 2'-deoxyguanosine (dG) which is a constituent component of DNA. This 8OHdG binds to adenine instead of cytosine which is the original base pair, and G: C → T: A
If not only transversion occurs but also mitochondrial DNA occurs at a certain frequency or more, intracellular energy supply is interrupted and cell death is induced. That is, d
G → 8OHdG oxidation induction is one of the most basic bio-oxidation phenomena directly linked to the lifespan of cells and individuals, and is useful as a measure of oxidative action on genes.

Example 1 In white birch sap as the first component,
As a second component, water-soluble nucleoprotein produced by the following method was added in the form of dry powder in an amount of 75 mg in terms of nucleic acid per healthy beverage, and the healthy beverage of Example 1 (health of the first invention of the present invention was added. Beverage) was obtained. <Production of Water-Soluble Nuclear Protein> 2500 g of frozen salmon Shirako was thawed to remove skins, muscles, blood vessels, etc., and then blood was drained and washed with water. Then, the salmon Shiroko was pulverized together with 1000 ml of water, 2.5 g of protease (manufactured by NOVO) was added, and enzyme treatment was carried out at 44 to 47 ° C. and pH 6.0 to 6.3 for 4 hours while stirring. Subsequently, the treated liquid is treated at 70 ° C.
The temperature was raised to 2.5 g, and 2.5 g of nuclease (manufactured by Amano Co., Ltd.) was added, and the enzymatic decomposition treatment was carried out for 4 hours at pH 5.0 to 5.5 while stirring. After the treatment, the temperature of the liquid was raised to 85 ° C. to deactivate the remaining protease and nuclease. The obtained product liquid is cooled to 40 to 50 ° C., continuously fed to a decanter to separate a clarified liquid and spray-dried, and in the form of powder, water-soluble low molecular weight nuclear protein I got a nuclear protein.
The properties of the obtained water-soluble nucleoprotein are as shown in Table 1.

[0028] Fig. 1 shows (8OHdG /) for each of the cases where the birch sap alone and the mixture of the birch sap and the water-soluble nucleoprotein (healthy beverage of Example 1) were used as test liquids in the efficacy evaluation test. dG) × 10000 (white triangles indicate no ultraviolet irradiation, black triangles indicate ultraviolet irradiation). The horizontal axis of FIG. 1 shows the presence or absence of ultraviolet irradiation, and the vertical axis of FIG. 1 shows the value of (8OHdG / dG) × 10000. In FIG. 1, in the case of only birch sap, (8OHdG /
The value of dG) × 10000 is 3.8, and the value of (8OHdG / dG) × 10000 after 120 minutes has passed from the start of the test is 6527.3.
The value of (8OHdG / dG) x 10000 is about 17!
It has increased 20 times. On the other hand, in the case of the mixture of white birch sap and water-soluble nucleoprotein (healthy beverage of Example 1), the value of (8OHdG / dG) × 10000 at the start of the test was 11.
9, 120 minutes after the start of the test (8OHdG / d
The value of G) × 10000 is 298.7, and (8OHdG / dG) × 1000 is obtained by irradiation with ultraviolet rays for 120 minutes.
The value of 0 increased about 25.1 times. Therefore, it is considered that by adding an appropriate amount of the water-soluble nucleoprotein to the white birch sap, the oxidative effect of the white birch sap on the gene was reduced to about 1/70 (25.1 / 1720) as compared with the case of the white birch sap alone. , Water-soluble nucleoprotein surprisingly reduces the oxidative effect of birch sap on genes.

Example 2: Birch sap as the first component,
The health drink of Example 2 (health drink of the present invention 2) was obtained by mixing antioxidant water as a second component in a predetermined ratio. FIG. 2 shows pure water (white circles), white birch sap (black circles) and antioxidant water (black squares) as test liquids in the efficacy evaluation test.
The time-dependent change of 8OHdG / dG is shown for each of the above. The horizontal axis of FIG. 2 shows the elapsed time (minutes) after the start of the test (the start of ultraviolet irradiation of 254 nm), and the vertical axis of FIG. 2 shows the value of (8OHdG / dG) × 10000. Figure 2
Therefore, the value of (8OHdG / dG) × 10000 of antioxidant water is lower than the value of (8OHdG / dG) × 10000 of pure water, and (8OHdG / dG) × 1 of white birch sap.
The value of 0000 is (8OHdG / d
It can be seen that it is much higher than the value of G) × 10000.

In FIG. 3, a value of (8 OHdG / dG) × 10000 (white bar) for the birch sap (M) alone and the 80:20 (mass ratio) mixture of the antioxidant water (S) and the birch sap (M). Shows the case without UV irradiation, and the patterned bar shows the case with UV irradiation). In FIG. 3, only (8OHdG / d) of white birch sap (M) with UV irradiation
The value of (G) × 10000 is 3191.6, and the value of (8OHdG / dG) × 10000 of the 80:20 (4: 1) mixture of the same antioxidant water (S) and white birch sap (M) is 331.
8 80 with antioxidant water (S) and white birch sap (M):
The amount of white birch sap (M) in the 20 mixture was 1/5 of that of the white birch sap (M) alone (8O
The value of HdG / dG) × 10000 is about 1/10, which is expected from the mixing ratio (8OHdG / dG) ×
The value is even lower than the value of 10,000. Therefore, if antioxidant water (S) and birch sap (M) are mixed in a suitable ratio, the oxidative effect of birch sap (M) on the gene can be obtained even in an atmosphere such as ultraviolet irradiation where the gene can be oxidized. It can be seen that it can be sufficiently reduced. That is, the addition of antioxidant water (S) is useful for reducing the oxidative effect of birch sap (M) on the gene, and the effect of adding antioxidant water (S) depends on the mixing ratio with the birch sap (M). Greater than the expected effect of addition, the addition of antioxidant water (S) is very effective in reducing the oxidative effect of birch sap (M) on genes.

Example 3: In the white birch sap as the first component,
As the second component, water-soluble nucleoprotein (which may be in the form of a solution or may be in the form of a dry powder) and antioxidant water are added and mixed at a predetermined ratio to birch sap, respectively. Thus, the health drink of the third invention of the present invention was obtained. The health drink of the present example has both the effects of the health drink of Example 1 and the effects of the health drink of Example 2, and the health maintenance / promotion effect is further enhanced.

[0032]

EFFECT OF THE INVENTION The health drink of the present invention has a water-soluble nucleoprotein, which is a component that has the health-maintaining / enhancing effect and natural flavor of birch sap as a natural drink and reduces the oxidative effect of birch sap on genes. Since it contains antioxidant water alone or in combination and may further contain further useful components as desired, compared to the case of birch sap alone, even in an atmosphere where UV irradiation, oxidant, etc. can oxidize the gene present, the birch tree against the gene It is possible to surprisingly reduce the oxidizing action of sap, and it is very useful as a health drink.

[Brief description of drawings]

FIG. 1 is a graph showing the presence or absence of ultraviolet irradiation in each test liquid in Example 1 of the present invention (8OHdG / dG).
It is a figure which shows the value of x10000.

FIG. 2 is a diagram showing a change with time of a value of (8OHdG / dG) × 10000 for each test liquid in Example 2 of the present invention.

[Fig. 3] Fig. 3 shows the presence or absence of ultraviolet irradiation in each test liquid in Example 2 of the present invention (8OHdG / dG).
It is a figure which shows the value of x10000.

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Claims (7)

[Claims] 1. A first component containing white birch sap as an active ingredient, and a second component for reducing the oxidative action of the first component on a gene, wherein the second component is a water-soluble nucleoprotein. A characteristic healthy drink. 2. The health drink according to claim 1, wherein the water-soluble nucleoprotein is a nucleoprotein solubilized by lowering the molecular weight of the salmon, trout or herring albino nucleoprotein. 3. The water-soluble nucleoprotein comprises 20% by mass or more of a low-molecular-weight oligonucleotide / nucleoside and oligopeptide obtained by treating nucleolar proteins in algae with nuclease and protease. The health drink according to claim 1 or 2, which is characterized. 4. A first component containing white birch sap as an active ingredient, and a second component for reducing an oxidizing action of the first component on a gene, wherein the second component has a nitrogen oxide concentration of 0.1 ppm or less. Health drink characterized in that it is antioxidized water obtained by treating the above water with a semiconductor catalyst in a magnetic field. 5. The health drink according to claim 4, wherein the semiconductor catalyst is an oxide semiconductor catalyst. 6. The health drink according to claim 5, wherein the oxide semiconductor catalyst is titanium dioxide. 7. A first component containing white birch sap as an active ingredient, and a second component for reducing the oxidative action of the first component on a gene, wherein the second component is a water-soluble nuclear protein and / or nitrogen. A health drink comprising antioxidant water obtained by treating water having an oxide concentration of 0.1 ppm or less with a semiconductor catalyst in a magnetic field.