JP2003294631A5 - - Google Patents

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JP2003294631A5
JP2003294631A5 JP2002097879A JP2002097879A JP2003294631A5 JP 2003294631 A5 JP2003294631 A5 JP 2003294631A5 JP 2002097879 A JP2002097879 A JP 2002097879A JP 2002097879 A JP2002097879 A JP 2002097879A JP 2003294631 A5 JP2003294631 A5 JP 2003294631A5
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Japan
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sample
target substance
state
specimen
prism
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JP2002097879A
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JP2003294631A (en
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Priority to JP2002097879A priority Critical patent/JP2003294631A/en
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Claims (3)

標的物質の状態を解析する分子蛍光解析方法であって、以下のことを具備する方法；
（１）試料標本中の標的物質に対して蛍光標識物質を付加することと、
（２）前記（１）の付加することと同時またはそれに続いて、前記試料標本に対してエバネッセント照明を行いながら、前記試料標本において生じる蛍光信号を複数の時点で継時的に検出することと、
（３）前記（２）の観察することにより得られた蛍光信号の変化を基に、前記標的物質の１分子当たりの挙動および／または状態の変化を解析し、それにより標的物質の状態を解析することと。A molecular fluorescence analysis method for analyzing the state of a target substance, comprising the following:
(1) adding a fluorescent labeling substance to a target substance in a sample specimen;
(2) At the same time as or after the addition of (1), continuously detecting fluorescence signals generated in the sample specimen at a plurality of time points while performing evanescent illumination on the sample specimen; ,
(3) Based on the change in the fluorescence signal obtained by the observation in (2) above, the behavior and / or state change per molecule of the target substance is analyzed, thereby analyzing the state of the target substance And to do. 前記試料標本がカバーガラス上に前記接着細胞を接着させることにより作成されること、前記顕微鏡が倒立顕微鏡であること、並びに前記エバネッセント照明を行うことが、前記カバーガラスの接着細胞の接着した面上にプリズムを配置し、前記プリズムにレーザ光を照射して前記カバーガラス内の境界面に前記レーザ光を全反射させることによって達成されることを特徴とする請求項１に記載の標的物質の状態を解析する方法。The sample specimen is prepared by adhering the adherent cells on a cover glass, the microscope is an inverted microscope, and the evanescent illumination is performed on the surface of the cover glass to which the adherent cells adhere. The state of the target substance according to claim 1, which is achieved by disposing a prism on the prism and irradiating the prism with laser light to totally reflect the laser light on a boundary surface in the cover glass. How to parse. 以下を具備する蛍光相関分析装置；
（１）レーザを照射するための光源と、
（２）前記光源と光路により連結され、前記光源より照射されたレーザを試料を支持するガラス板内の試料に近い境界面に全反射することによりエバネッセント波を形成し、検出の対象となる試料標本に対して前記エバネッセント波を照射するための照明系と、
（３）前記照明系により形成されたエバネッセント波により励起され生じた蛍光信号を検出するための光検出器。A fluorescence correlation analyzer comprising:
(1) a light source for irradiating a laser;
(2) A sample to be detected by forming an evanescent wave by being totally reflected on a boundary surface close to the sample in the glass plate supporting the sample, which is connected to the light source by the optical path An illumination system for irradiating the specimen with the evanescent wave;
(3) A photodetector for detecting a fluorescence signal generated by being excited by an evanescent wave formed by the illumination system.
JP2002097879A 2002-03-29 2002-03-29 Fluorescent method for analyzing molecule using evanescent illumination Pending JP2003294631A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2002097879A JP2003294631A (en) 2002-03-29 2002-03-29 Fluorescent method for analyzing molecule using evanescent illumination

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2002097879A JP2003294631A (en) 2002-03-29 2002-03-29 Fluorescent method for analyzing molecule using evanescent illumination

Publications (2)

Publication Number Publication Date
JP2003294631A JP2003294631A (en) 2003-10-15
JP2003294631A5 true JP2003294631A5 (en) 2005-09-15

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JP2002097879A Pending JP2003294631A (en) 2002-03-29 2002-03-29 Fluorescent method for analyzing molecule using evanescent illumination

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Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006113462A (en) * 2004-10-18 2006-04-27 Tohoku Univ Three-dimensional position tracking method for single particles
FR2882845B1 (en) 2005-03-04 2007-06-01 Somfy Sas ACTUATOR CONTROL METHOD
JP4572162B2 (en) * 2005-11-22 2010-10-27 株式会社ジーオングストローム Microscope equipment
JP5002813B2 (en) * 2007-02-06 2012-08-15 国立大学法人浜松医科大学 Fluorescence measuring device
JP5080186B2 (en) 2007-09-26 2012-11-21 富士フイルム株式会社 Molecular analysis photodetection method, molecular analysis photodetection device used therefor, and sample plate
JP5107003B2 (en) * 2007-11-22 2012-12-26 パナソニックヘルスケア株式会社 Evanescent wave generator and observation apparatus using the same
KR101158362B1 (en) 2011-04-20 2012-06-22 한국과학기술원 Method for analyzing single protein-protein interactions in whole cell lysates

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