JP2003238416A - Intestinal bacteria improving composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an intestinal bacteria improving composition. <P>SOLUTION: The composition contains acacia gum and an oligosaccharide. The composition may further contain erythritol. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a composition for improving enterobacteria.

[0002]

2. Description of the Related Art Recently, attention has been paid to the relationship between human health and intestinal bacteria, and research has been conducted to find various facts. For example, the intestinal bacterium Bacteroi
daceae, Bifidobacterium, La
Ctobacillus and the like have functions of synthesizing vitamins and proteins, assisting digestion and absorption, preventing the growth of foreign bacteria, and stimulating immune function, and contribute to the maintenance of human health. On the other hand, Escherichia coli, a lecithinase-positive Clostridium pertrin
genese, Staphylococcus and Pseu
Domonas is a disease such as diarrhea, constipation, liver damage, cancer, and hypertension in humans due to intestinal rot (formation of ammonia, hydrogen sulfide, amine, phenol), infectious disease, formation of carcinogens and toxins. And cause aging. Therefore, it is preferable to improve the bacteria in the intestine to generate a flora that is beneficial to the living body.

Therefore, various means have been considered until now. The most common method is mainly in the large intestine
Ingestion of oligosaccharide that grows dobacterium. However, oligosaccharides are not
Fidobacterium grows rapidly in the intestine,
Although the number of bacteria can be increased 10 times, it is also widely used by other bacterial species, so the effect of specifically growing Bifidobacterium during long-term intake (1 month or more) disappears, and oligosaccharide intake It returns to the previous flora. Furthermore, since oligosaccharides are low-molecular weight compounds, they easily cause diarrhea, which causes a problem for middle-aged and elderly layers with weak gastrointestinal tract, even if the amount is appropriate for the younger layers. Bifidobacterium is also administered in a stable dosage form in the stomach and small intestine.
Even if Bifidobacterium reaches the large intestine, Bifidobacterium cannot proliferate in the large intestine unless the environment suitable for its growth is adjusted by, for example, diet.

Further, a method of improving the intestinal flora by administering gum arabic alone has been proposed (Wyatt,
G. M. Et al, British Journal of
Nutrition, 55, 261-266 (198)
See 6)). However, with this method, Bifid in the intestine
It takes 4 to 5 weeks for the bacteria to grow 10 times in terms of the number of bacteria. Therefore, few conventional methods are satisfactory.

[0005]

The present invention provides a composition for improving intestinal bacteria which is superior to the conventional ones.

[0006]

The present invention relates to a composition for improving enteric bacteria containing acacia gum and oligosaccharide. The present invention also relates to an intestinal bacterium improving composition in which erythritol is combined with acacia gum and oligosaccharide.

The acacia gum used in the present invention is also called gum arabic or gum arabic, and is a sap gum collected from the legume family Acacia senegal which grows in the tropical region. Naturally, it is a polysaccharide that is neutral or slightly acidic and consists of arabinogalactan as the main chain. Acacia gum has been reported to have a blood lipid regulating effect, and for example, phospholipids, triglycerides, total cholesterol and the like are improved after administration of 25 g of acacia gum per day for 3 weeks (McLean).
-Rose, A .; H. Et al., Am. J. Clin. Nut
r. , 37, 368 (1983)).

The acacia gum used in the composition of the present invention preferably has a water content of 12% by weight or less. If the water content is more than 12% by weight, when it is mixed with other raw materials and made into a powdered food product, there is a problem in storage and a bad taste may occur.

The oligosaccharides used in the composition of the present invention include sucrose-based oligosaccharides (for example, fructooligosaccharide, galactosylsucrose, coupling sugar, theanderose, palatinose, trehalulose,
Raffinose), lactose-based oligosaccharides (eg galacto-oligosaccharides, lactulose etc.), oligosaccharides derived from starch and other polysaccharides (eg isomaltooligosaccharides, kencholigosaccharides, trehalose, xylooligosaccharides, soybean oligosaccharides etc.) Including. These oligosaccharides are
Each or a combination can be used.

In the composition of the present invention, when gum arabic is administered alone, Bifidobacte in the large intestine
It takes about 4 to about 5 weeks for the number of rium bacteria to increase to 11 with 10 logarithmic plants per 1 g of feces, but when oligosaccharides are used together, about 1 to about 3 weeks per 1 g of logarithmic plants are combined with oligosaccharides. 1
Becomes 1. Bifidobacterium in the large intestine
The number of um bacteria is usually 10.0 in healthy adults (42 cases)
It is ± 0.8, but it is 9.4 ± 0.8 for humans (54 cases) with a small number of bacteria (Innan, Kiriyama, ed., “Dietary Fiber”, pages 251-280, Daiichi Shuppan Co., Ltd. )
(1995)).

The daily intake of the composition of the present invention is about 5 to about 25 g, preferably about 6 to about 23 g, and most preferably about 7 to about 20 g, once or several times a day. Ingest separately. When combined with oligosaccharides, about 0.5 to about 3 g of oligosaccharide can be used per day, in which case the amount of gum acacia can be reduced to about 3 to about 10 g per day. Intestinal flora improving effect, that is, Bif in the large intestine
The number of bacteria of idobacterium was 10-11 by logarithmic planting.
The effective intake of oligosaccharides required to increase the amount of the sugar is, for example, raffinose, 3 g / day (Sayama, Japan Food Science, 32, No. 9).
(See (1993)), its effective intake can be reduced to 1/2 to 1/4 by using it in combination with gum acacia, and its maximum ineffective amount (the maximum amount that does not cause diarrhea when ingested, In the case of raffinose, it can be reduced to 1/6 or less of 10 g / day), and it is possible to eliminate the risk of diarrhea even in a person with a weak intestine, such as an elderly person.

The composition of the present invention can be used as it is or in the form of food. As a sweetener, erythritol has no hygroscopicity, has a sweetness very similar to sucrose, and when ingested, it is absorbed in the small intestine and excreted in urine, and therefore does not pass through the large intestine. Therefore, the energy value is set to 0 kcal / 100 g. Therefore, it does not cause diarrhea like other sugar alcohols without affecting the intestinal flora. Since it also has the effect of coating the unique flavor of acacia gum itself, it is most suitable as a sweetener when the composition of the present invention is used in the form of food. Examples of the form of foods include noodles, confectionery, breads, cereal foods, frozen desserts, frozen desserts, soups, processed marine products, processed meat products, beverages, dairy products and the like. Furthermore, a flavoring agent (for example, vanilla, chocolate, etc.), a flavor (for example, orange, strawberry, etc.), a pigment, etc. can be added to enhance the palatability.

The composition of the present invention has no toxicity.
In addition, conventional administration of oligosaccharides alone loses its effect when ingested for about one month or more, as described above.
Further, in the case of administration of acacia gum alone, it takes 4-5 weeks to grow the number of bacteria as described above, whereas in the composition of the present invention, the number of bacteria increases in a short period of 1-3 weeks, and it takes several months. Maintains the number of Bifidobacterium at 11 levels in logarithmic plant even after continuous administration, while increasing other useful bacteria such as Lactbacillus to improve intestinal bacterial flora and maintain excellent physical condition can do. Moreover, even if it is used for a long period of time, inconvenience such as diarrhea does not occur.

[0014]

Example 1 A 65-year-old man was used as a test subject, and 15 g of acacia gum (Cleo gum (trademark) sold by Japan, Creo International, Inc.) having a water content of 10% by weight, 2 g of raffinose and 1 g of erythritol were added to 200 mL of water. What was melt | dissolved in was ingested at 9:00 every morning. After 5 weeks, the subject's feces were collected. After 24 hours of refrigeration, samples were used at 37 ° C under anaerobic conditions at 72 ° C using 9 kinds of agar plate medium for anaerobic bacteria.
Incubated for hours. The agar plate medium for anaerobic bacteria was as follows. (1) EG agar medium (Eiken) [anaerobic bacteria (non-selected)], (2) BL agar medium (Japanese water) [anaerobic bacteria (non-selected)], (3) BS agar medium (base Japanese water) (BL agar medium) (Bifidobacterium), (4) N
BGT agar medium (base Japanese water EG agar medium) (Bac
teroidaceae), (5) BBE agar medium (Far East) (B. fragilis group), (6) Neomyc
in Neglar agar (lecithinase (+) Cl
ostridium), (7) Modified FM medium (Nissui)
(Fusobacterium), (8) ES agar medium (base Eiken EG agar medium) (Eubacteriu)
m), (9) VS agar medium (Veillonella)
Megasphaera). On the other hand, using 7 kinds of agar plate medium for aerobic bacteria, the cells were cultured under aerobic conditions at 37 ° C for 24-72 hours. The agar plate medium for aerobic bacteria was as follows. (1) Trypticase Soy Blo
od Agar (baseBBL) (aerobic bacteria (non-selective)), (2) DHL agar medium (Eiken) (Entero
(3) TATAC agar medium (Streptococcus Enterococcus)
cus), (4) PEES agar medium (base Eiken Stahirococcus 110) (Staphylococcu)
s), (5) NAC agar medium (Eiken) (Pseudomonas aeruginosa),
(6) Potato dextrose agar medium (base Eiken)
(Yeast), (7) modified LBS agar medium (baseBB
L) (Lactobacillus).

After the completion of the culture, the shape and number of colonies growing on each medium were recorded and Gram staining was performed. The bacterial group was determined by the shape of the colony, Gram's stainability, and the morphology of the bacteria. The number of bacteria is indicated as a percentage of the total number of bacteria per 1 g of hydrous feces and the logarithmic plant number of the number of bacteria.

[0016] In addition, data was also obtained on feces one month before the intake of acacia gum and raffinose, which was used as a control. That is, in the case of the control, the composition of the present invention was not ingested.

In the Bifidobacterium, the ratio of the total number of feces to the total number of feces was 49%, which was 5% in the control.
In Lactbacillus, the control increased from 2% to 8%. Bacter
Although iodaceae is generally the main intestinal flora in humans, the control was 47%, whereas 38
% Has been reduced. In Eubacterium, the control was 30%, while it was reduced to 6%.

The logarithmic plant number of the total number of feces was 1 for the control.
It was 0.9, but became 11.5. Lecithi
case positive C. Perfringens was 3.5 in the control but was not detected.

In this case, an acacia gum having a water content of 13% by weight was tried to be used, but it had a bitter taste, was not edible, and could not be used for the experiment.

Example 2 The method of Example 1 was repeated, except that gum acacia 8.
5 g, galactooligosaccharide 1.5 g, erythritol 1 g
Was used.

In the measurement after 3 weeks, the ratio of the number of bacteria of each bacterium to the total number of stools was determined by Bifidobacterium.
In um, the control was 5%, while 59
%, And the logarithm of the total number of feces was 9.6 in the control, but became 10.6. It was Also,
For Lactobacillus, the control was 0% and the logarithmic plant was 0, whereas it was also 0% and the logarithmic plant was 0. In addition, lecithinase-positive C.I. No perfringens were detected.

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Claims (2)

[Claims] 1. An intestinal bacterium improving composition comprising acacia gum and an oligosaccharide. 2. The composition of claim 1, further comprising erythritol.