JP2003235357A - Agricultural and horticultural physiological activator - Google Patents
Agricultural and horticultural physiological activatorInfo
- Publication number
- JP2003235357A JP2003235357A JP2002036577A JP2002036577A JP2003235357A JP 2003235357 A JP2003235357 A JP 2003235357A JP 2002036577 A JP2002036577 A JP 2002036577A JP 2002036577 A JP2002036577 A JP 2002036577A JP 2003235357 A JP2003235357 A JP 2003235357A
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- JP
- Japan
- Prior art keywords
- cinnamic acid
- aqueous solution
- diluted
- mixed aqueous
- agricultural
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Cultivation Of Plants (AREA)
- Catching Or Destruction (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は桂皮酸および／また
は桂皮酸誘導体の高濃度水溶液からなる農・園芸用生理
活性剤に関し、さらに詳しくは、桂皮酸および／または
桂皮酸誘導体の高濃度水溶液からなる開花促進剤、花蕾
促成剤、害虫忌避剤、抗菌剤、防黴剤等として有用な農
・園芸用生理活性剤に関する。TECHNICAL FIELD The present invention relates to an agricultural / horticultural bioactive agent comprising a high-concentration aqueous solution of cinnamic acid and / or a cinnamic acid derivative, and more specifically, a high-concentration aqueous solution of cinnamic acid and / or a cinnamic acid derivative. The present invention relates to an agricultural / horticultural bioactive agent useful as a flowering accelerator, flower bud promoting agent, pest repellent, antibacterial agent, antifungal agent, etc.
【０００２】[0002]
【従来の技術】桂皮酸、コーヒー酸などのリグニン生合
成経路中間物質が、セントポーリアなどの開花促進効果
を有することが明らかにされ、これらの物質を開花促進
剤として使用することが提案されている（特開平８−２
５９４０８号公報、特開平１０−２７３４０４号公
報）。また、上記の桂皮酸、コーヒー酸などのリグニン
生合成経路中間物質は、種々な生理活性作用を有してお
り、例えば、花卉野菜用花蕾促成剤、害虫忌避剤、抗菌
剤、防黴剤などとしても農・園芸用に利用されている。2. Description of the Related Art It has been clarified that lignin biosynthetic pathway intermediates such as cinnamic acid and caffeic acid have a flowering promoting effect such as Saintpaulia, and it has been proposed to use these substances as flowering promoting agents. (JP-A-8-2
59408, JP-A-10-273404). In addition, the lignin biosynthetic pathway intermediates such as cinnamic acid and caffeic acid have various physiologically active actions, and for example, flower bud promoting agents for flower vegetables, pest repellents, antibacterial agents, antifungal agents, etc. It is also used for agriculture and gardening.
【０００３】桂皮酸やコーヒー酸などを上記の用途で実
際に使用する場合、それらの使用形態は作業効率の点か
ら液状タイプが圧倒的に有利である。従来からよく用い
られている液状タイプの栄養剤や肥料液などの植物賦活
剤は、通常、濃縮された原液を使用に際して適宜希釈し
て鉢植えなどの植物に散布して使用されている。従っ
て、できるだけ濃縮度の高い原液を使うことが、保管ス
ペースを少なくし、輸送効率を高めるとともに、使用者
の使い勝手を向上させるなど、多くのメリットがある。
また、溶剤としては水の使用が望まれる。When cinnamic acid, caffeic acid, etc. are actually used in the above-mentioned applications, the liquid type is overwhelmingly advantageous from the viewpoint of work efficiency. Conventionally used plant activators such as liquid type nutrients and fertilizer solutions are usually used by appropriately diluting a concentrated stock solution and spraying it on plants such as potted plants. Therefore, using a stock solution having a high degree of concentration has many advantages such as reducing the storage space, improving the transportation efficiency, and improving the usability for the user.
Further, it is desirable to use water as the solvent.
【０００４】しかしながら、桂皮酸、コーヒー酸などの
生理活性物質は、水への溶解性は極めて低く（例えば、
桂皮酸の水への溶解度は０．５４６ｇ／リットル（２５
℃）、コーヒー酸は水に難溶である。）、これらの物質
を使用した液状タイプの各種生理活性剤を製造する際に
は、希薄な溶液を大量に作ることを強いられると同時
に、これらの製品を保管するための広大なスペースが必
要であった。また、実用的に有効な生理活性を発揮させ
ためには、桂皮酸、コーヒー酸などの生理活性物質の飽
和水溶液以上の濃度が必要な場合があり、そのような場
合に安全で有効な手段が無かった。However, physiologically active substances such as cinnamic acid and caffeic acid have extremely low solubility in water (for example,
The solubility of cinnamic acid in water is 0.546 g / liter (25
C), caffeic acid is poorly soluble in water. ), When manufacturing various liquid-type bioactive agents using these substances, it is necessary to make a large amount of dilute solution, and at the same time, a vast space for storing these products is required. there were. Further, in order to exert a practically effective physiological activity, a concentration of a physiologically active substance such as cinnamic acid or caffeic acid which is equal to or higher than a saturated aqueous solution may be required, and in such a case, a safe and effective means is required. There was no
【０００５】[0005]
【発明が解決しようとする課題】本発明は上記の事情に
鑑みてなされたものであり、本発明の目的は保管スペー
スが削減され、輸送効率や作業効率が向上し、安全で環
境に優しい、桂皮酸および／またはその誘導体の高濃度
水溶液からなる農業・園芸用生理活性剤を提供すること
である。本発明者らは、上記の目的を達成するために鋭
意研究を重ねた結果、水酸化カリウム、炭酸カリウム、
トリポリリン酸ナトリウム、酢酸ナトリウムなどの水に
溶解してアルカリ性を呈する物質が、桂皮酸などを水に
高濃度で溶解させるための溶解助剤として作用し、これ
らの溶解助剤を用いて調製した高濃度水溶液が生理活性
を有し、希釈も可能であり、 農業・園芸用生理活性剤
として有効であることを見出し、本発明を完成するに至
った。The present invention has been made in view of the above circumstances, and an object of the present invention is to reduce storage space, improve transportation efficiency and work efficiency, and be safe and environmentally friendly. It is intended to provide an agricultural / horticultural bioactive agent comprising a high-concentration aqueous solution of cinnamic acid and / or a derivative thereof. The present inventors have conducted extensive studies to achieve the above-mentioned object, and as a result, potassium hydroxide, potassium carbonate,
Sodium tripolyphosphate, sodium acetate, and other substances that exhibit alkalinity when dissolved in water act as a solubilizing agent for dissolving cinnamic acid and the like in water at a high concentration. The inventors have found that a concentrated aqueous solution has bioactivity, can be diluted, and is effective as a bioactive agent for agriculture and horticulture, and has completed the present invention.
【０００６】[0006]
【課題を解決するための手段】かかる本発明によれば、
桂皮酸および／または桂皮酸誘導体が、その水に対する
飽和溶解度を超える濃度に水に溶解してなることを特徴
とする農・園芸用生理活性剤が提供される。According to the present invention,
Disclosed is a bioactive agent for agricultural and horticultural use, characterized in that cinnamic acid and / or a cinnamic acid derivative is dissolved in water to a concentration exceeding its saturation solubility in water.
【０００７】本発明の桂皮酸などのリグニン生合成経路
代謝中間物質の高濃度水溶液は、希釈用原液とし、これ
を適当な濃度に希釈した場合にも、桂皮酸などの有する
生理活性は保持されるので、セントポーリアなどの花卉
に対する開花促進剤、ブロッコリー、カリフラワーなど
の花卉野菜の花蕾促成剤、害虫忌避剤、抗菌剤、防黴剤
やその他の植物用生理活性剤として、桂皮酸などと水の
みからなる極めて低濃度の水溶液に代えて使用すること
により、使用効率を大幅に改善することができる。The high-concentration aqueous solution of the lignin biosynthetic pathway metabolic intermediate such as cinnamic acid of the present invention is used as a stock solution for dilution, and the physiological activity possessed by cinnamic acid is retained even when diluted to an appropriate concentration. Therefore, as a flowering promoter for flowers such as Saintpaulia, flower bud accelerating agent for flower vegetables such as broccoli and cauliflower, pest repellent, antibacterial agent, fungicide and other bioactive agents for plants, only cinnamic acid and water are used. By using it instead of the extremely low-concentration aqueous solution consisting of, the use efficiency can be greatly improved.
【０００８】[0008]
【発明の実施の形態】次に本発明について詳細に説明す
る。本発明で使用するリグニン生合成経路中間物質自体
はいずれも公知の化合物であり、桂皮酸、ｐ−クマル酸
およびコーヒー酸から選択される。これらは単独でも混
合しても使用することができる。又、これらは合成品で
も天然品でもよい。特に好ましいのは、桂皮酸およびコ
ーヒー酸である。尚、これらの物質の中には、根の成育
を阻害する恐れのある物質もあるが、そのような物質は
根が充分に発育した後に使用することが好ましい。BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below. The lignin biosynthetic pathway intermediates used in the present invention are all known compounds and are selected from cinnamic acid, p-coumaric acid and caffeic acid. These can be used alone or in a mixture. Further, these may be synthetic products or natural products. Particularly preferred are cinnamic acid and caffeic acid. Although some of these substances may inhibit root growth, it is preferable to use such substances after the root has sufficiently developed.
【０００９】本発明の生理活性剤である桂皮酸などの高
濃度水溶液は、桂皮酸などが、その水に対する２５℃の
飽和溶解度（最大溶解度）を超える濃度に水に溶解して
いることが特徴である。このような水溶液は、水溶性の
溶解助剤を使用することで製造することができる。本発
明で使用する溶解助剤は、その水溶液がアルカリ性を呈
する弱酸と強塩基の塩又は塩基であればいずれも使用可
能であるが、ｐＨ緩衝作用を示し、環境汚染の恐れが少
なく、人体に安全であるものが好ましい。弱酸と強塩基
の塩としては、例えば、トリポリリン酸ナトリウム、ト
リポリリン酸カリウム、ポリリン酸ナトリウム、ポリリ
ン酸カリウム、リン酸３ナトリウム、リン酸３カリウ
ム、リン酸水素２カリウム、リン酸水素２ナトリウムな
どのリン酸塩、炭酸カリウム、炭酸ナトリウム、酢酸ナ
トリウム、酢酸カリウムなどが、塩基としては、例え
ば、水酸化ナトリウム、水酸化カリウムなどが好ましい
ものとして挙げられる。特に好ましい溶解助剤として
は、食品添加物に指定されているトリポリリン酸ナトリ
ウム、水酸化カリウム、炭酸カリウム、酢酸ナトリウム
などが用いられる。The high-concentration aqueous solution of cinnamic acid or the like, which is a physiologically active agent of the present invention, is characterized in that cinnamic acid or the like is dissolved in water at a concentration exceeding its saturated solubility (maximum solubility) at 25 ° C. in water. Is. Such an aqueous solution can be produced by using a water-soluble solubilizing agent. The solubilizing agent used in the present invention may be any salt or base of a weak acid and a strong base whose aqueous solution exhibits alkalinity, but exhibits a pH buffering action, is less likely to cause environmental pollution, and has a human body. Those that are safe are preferred. Examples of the salt of a weak acid and a strong base include sodium tripolyphosphate, potassium tripolyphosphate, sodium polyphosphate, potassium polyphosphate, 3 sodium phosphate, 3 potassium phosphate, 2 potassium hydrogen phosphate, 2 sodium hydrogen phosphate and the like. Phosphates, potassium carbonate, sodium carbonate, sodium acetate, potassium acetate and the like can be mentioned, and preferable examples of the base include sodium hydroxide, potassium hydroxide and the like. As a particularly preferable solubilizing agent, sodium tripolyphosphate, potassium hydroxide, potassium carbonate, sodium acetate and the like specified in food additives are used.
【００１０】桂皮酸などの高濃度水溶液を製造するに際
しては、例えば、溶解助剤の水溶液を予め調製し、これ
に桂皮酸などを加えてよく混合し、溶解させることによ
り容易に桂皮酸などの高濃度水溶液を得ることができ
る。溶解助剤の使用量は、特に限定されないが、桂皮酸
などに対して３５〜３００重量％となる量が好ましい。
このように溶解助剤を使用することで桂皮酸などの含有
量が、室温での水に対する最大溶解度を超え、２５重量
％以下の高濃度水溶液が得られる。高濃度水溶液の保管
スペース、輸送効率などの点から、好ましい濃度は０．
５〜２５重量％である。In producing a high-concentration aqueous solution of cinnamic acid or the like, for example, an aqueous solution of a solubilizing agent is prepared in advance, and cinnamic acid or the like is added thereto and mixed well to dissolve the cinnamic acid or the like. A highly concentrated aqueous solution can be obtained. The amount of the solubilizing agent used is not particularly limited, but is preferably an amount of 35 to 300% by weight with respect to cinnamic acid and the like.
By using the solubilizing agent in this way, the content of cinnamic acid and the like exceeds the maximum solubility in water at room temperature, and a highly concentrated aqueous solution of 25% by weight or less can be obtained. From the viewpoint of storage space for highly concentrated aqueous solution, transportation efficiency, etc., the preferred concentration is 0.1.
It is 5 to 25% by weight.
【００１１】製造方法の一例を挙げると、溶解助剤が弱
酸と強塩基の塩である場合、例えば、１Ｎの酢酸ナトリ
ウム水溶液（ｐＨ８．９）１００ｍｌに０．５ｇの桂皮
酸を溶解させると、０．５重量％の桂皮酸水溶液（ｐＨ
６．１）を得ることができる。又、溶解助剤が塩基であ
る場合、例えば、０．５Ｎの水酸化ナトリウム水溶液１
００ｍｌに７．３ｇの桂皮酸を溶解させることで７．３
重量％の桂皮酸水溶液（ｐＨ７．２）を得ることができ
る。使用に際しては、これを例えば１０倍に希釈して約
０．７重量％の桂皮酸水溶液（ｐＨ６．６）としたり、
１００倍に希釈して約０．０７重量％の桂皮酸水溶液
（ｐＨ６．３）として使用することができる。As an example of the production method, when the dissolution aid is a salt of a weak acid and a strong base, for example, when 0.5 g of cinnamic acid is dissolved in 100 ml of a 1N sodium acetate aqueous solution (pH 8.9), 0.5% by weight cinnamic acid aqueous solution (pH
6.1) can be obtained. When the solubilizing agent is a base, for example, 0.5N sodium hydroxide aqueous solution 1
7.3g by dissolving 7.3g cinnamic acid in 00ml
A wt% cinnamic acid aqueous solution (pH 7.2) can be obtained. At the time of use, for example, this is diluted 10 times to prepare an approximately 0.7% by weight cinnamic acid aqueous solution (pH 6.6),
It can be diluted 100-fold and used as an aqueous solution of about 0.07% by weight cinnamic acid (pH 6.3).
【００１２】溶解助剤が、例えば、炭酸カリウム、トリ
ポリリン酸ナトリウム、酢酸ナトリウムなどの弱酸と強
塩基の塩である場合、これらの塩の水溶液中では、これ
らの塩は陽イオンと陰イオンに電離している。ここに、
例えば、桂皮酸が加えられると桂皮酸由来のプロトンが
溶解助剤由来の陰イオンに消費され、水溶液中の桂皮酸
の電離が促進される方向に動き、桂皮酸イオンが生じ
て、これが水溶液中に溶け出し、桂皮酸全体の溶解量が
増加する。When the solubilizing agent is a salt of a weak acid and a strong base such as potassium carbonate, sodium tripolyphosphate, sodium acetate, etc., in an aqueous solution of these salts, these salts are ionized into cations and anions. is doing. here,
For example, when cinnamic acid is added, protons derived from cinnamic acid are consumed by anions derived from solubilizers, and the ionization of cinnamic acid in an aqueous solution is promoted to generate cinnamic acid ions, which are The amount of cinnamic acid dissolved increases.
【００１３】例えば、酢酸ナトリウムを例に示すと、酢
酸ナトリウム（ＣＨ3ＣＯＯＮａ）は水溶液中で下記の
式（１）のように電離し、平衡は右に偏っている。
また、水溶液中で桂皮酸（Ｃ6Ｈ5−ＣＨ＝ＣＨ−ＣＯＯ
Ｈ）は下記の式（２）のように電離するが、平衡は左に
偏っている。
桂皮酸由来のＨ+は、酢酸イオンＣＨ3ＣＯＯ-と反応し
て酢酸ＣＨ3ＣＯＯＨを生じる。その結果、桂皮酸由来
のＨ+は、酢酸イオンにより消費され、その濃度が減少
するので、式（２）式の平衡は右に動き、桂皮酸は桂皮
酸イオンＣ6Ｈ5−ＣＨ＝ＣＨ−ＣＯＯ-となって水中に
溶け出す。For example, taking sodium acetate as an example, sodium acetate (CH 3 COONa) is ionized in an aqueous solution as shown in the following formula (1), and the equilibrium is biased to the right. Also, in an aqueous solution, cinnamic acid (C 6 H 5 —CH═CH—COO
H) is ionized as in the following equation (2), but the equilibrium is biased to the left. H + derived from cinnamic acid reacts with acetate ion CH 3 COO − to produce acetate CH 3 COOH. As a result, H + derived from cinnamic acid is consumed by the acetate ion, and its concentration decreases. Therefore, the equilibrium of the formula (2) moves to the right, and cinnamic acid reacts with the cinnamic acid ion C 6 H 5 —CH═CH. It becomes -COO - and dissolves in water.
【００１４】本発明の桂皮酸などの高濃度水溶液からな
る生理活性剤は、その使用に際しては、一応の目安とし
て、例えば純分１０重量％程度に調製した高濃度水溶液
を、１０〜２０，０００倍程度に希釈して使用する。本
発明の生理活性剤の使用形態は、特に制限されず、例え
ば、希釈高濃度水溶液のままで使用したり、必要によ
り、肥料や他の植物活性剤やその他の添加剤などと混合
して用いることができる。また、本発明の生理活性剤が
有効に使用される対象の植物は、殆んどすべての花卉、
例えば、セントポーリア、アネモネ、水仙、デージー、
クリサンセマム、パンジー、ビオラ、蘭、ペチュニア、
シクラメン、ユリ、ダイナンサス、キク、ヒヤシンス、
カーネーション、バラ、ポーチュラカ、ファレノプシス
リンゴ、フユサンゴなどやブロッコリー、カリフラワー
などの花卉野菜などであり、植物の種類は特に限定され
るものではない。The physiologically active agent of the present invention comprising a high-concentration aqueous solution such as cinnamic acid should be used as a rough guide, for example, to prepare a high-concentration aqueous solution having a pure content of about 10% by weight in an amount of 10 to 20,000. Dilute it about twice before use. The form of use of the physiologically active agent of the present invention is not particularly limited, and for example, it can be used as a diluted high-concentration aqueous solution as it is or, if necessary, mixed with a fertilizer, another plant active agent, or other additives. be able to. In addition, the target plants for which the bioactive agent of the present invention is effectively used are almost all the flowers,
For example, Saintpaulia, anemone, daffodils, daisies,
Chrysanthemum, pansy, viola, orchid, petunia,
Cyclamen, lily, dynanthus, chrysanthemum, hyacinth,
Carnations, roses, portulaca, phalaenopsis apples, fusango corals, and floristic vegetables such as broccoli and cauliflower, etc., and the type of plant is not particularly limited.
【００１５】本発明の生理活性剤の使用量は、特に限定
されず、例えば、セントポーリア用開花促進剤として使
用する場合には、特に効果的な使用量は、培地（土壌も
含めて）の合計量１００重量部あたり桂皮酸などとして
０．００００１〜０．０２重量部となる量である。使用
量が多すぎる場合はセントポーリアの成育に悪い影響を
与えることがあり、少なすぎる場合には十分な開花促進
効果が得られないことがある。The amount of the physiologically active agent of the present invention used is not particularly limited. For example, when it is used as a flowering promoter for Saintpaulia, the particularly effective amount is the total amount of the medium (including soil). The amount is 0.00001 to 0.02 part by weight per 100 parts by weight of cinnamic acid. If the amount used is too large, it may adversely affect the growth of Saintpaulia, and if it is too small, it may not be possible to obtain a sufficient flowering promoting effect.
【００１６】また、害虫忌避剤として使用する場合に
は、桂皮酸などの含有量が０．０１〜０．５重量％の水
溶液として使用することが効果的である。桂皮酸などの
含有量が少なすぎると十分な効果が発揮されない場合が
あり、多すぎると植物体に悪影響を与えることがある。
また、花卉の種類にもよるが、切花用の花瓶内容液では
０．０１〜０．０５重量％程度の水溶液が好ましく、散
布用では０．１〜０．５重量％程度の水溶液が好まし
い。When used as a pest repellent, it is effective to use it as an aqueous solution containing 0.01 to 0.5% by weight of cinnamic acid or the like. If the content of cinnamic acid or the like is too small, the sufficient effect may not be exhibited, and if the content is too large, it may adversely affect the plants.
Although depending on the type of flower, the vase content liquid for cut flowers is preferably an aqueous solution of about 0.01 to 0.05% by weight, and the spraying is preferably an aqueous solution of about 0.1 to 0.5% by weight.
【００１７】[0017]
【実施例】次に実施例及び参考例を挙げて本発明を更に
具体的に説明する。尚、文中の部又は％は重量基準であ
り、開花促進剤、花蕾促成剤、害虫忌避剤などの生理活
性剤の使用量は培地（土壌も含めて）の合計量１００重
量部に対する量である。EXAMPLES The present invention will be described in more detail with reference to Examples and Reference Examples. The parts or% in the text are based on weight, and the amount of bioactive agent such as flowering promoter, flower bud promoting agent, pest repellent, etc. is based on 100 parts by weight of the total amount of the medium (including soil). .
【００１８】実施例１
（１）高濃度桂皮酸水溶液の製造
２０℃にて蒸留水４００ｍｌに炭酸カリウム５０ｇを溶
解し、これに桂皮酸５０ｇを加えて攪拌、溶解した。蒸
留水にて全量を５００ｍｌとし、桂皮酸純分１０％の桂
皮酸／炭酸カリウム混合水溶液を得た。次に、この混合
水溶液を蒸留水にて２０，０００倍に希釈し、桂皮酸純
分０．０００５％の桂皮酸／炭酸カリウム混合水溶液の
希釈液を得た。この希釈液のｐＨを測定したところ８．
９であった。Example 1 (1) Preparation of high-concentration cinnamic acid aqueous solution At 20 ° C., 50 g of potassium carbonate was dissolved in 400 ml of distilled water, to which 50 g of cinnamic acid was added and stirred to dissolve. The total amount was adjusted to 500 ml with distilled water to obtain a cinnamic acid / potassium carbonate mixed aqueous solution having a cinnamic acid pure content of 10%. Next, this mixed aqueous solution was diluted 20,000 times with distilled water to obtain a diluted solution of a cinnamic acid / potassium carbonate mixed aqueous solution having a cinnamic acid pure content of 0.0005%. When the pH of this diluted solution was measured, 8.
It was 9.
【００１９】（２）セントポーリアの開花促進試験
人工気象器内で育成したセントポーリア（ノースカロラ
イナ）の苗を小鉢（直径:１１ｃｍ、深さ：８ｃｍ）に
植え替え、液体肥料を加えて、２５℃、２，０００ルッ
クス、１６時間照射／日の条件の人工気象器内に２週間
放置した。その後、鉢の受皿（３ポット）に上記の桂皮
酸純分０．０００５％の希釈混合水溶液を流し込み、受
皿に常にこの溶液が満ちている状態に保つように時々液
を補充しながら栽培した。希釈混合水溶液の代わりに水
を用いた場合を対照として同時に栽培した。栽培２６日
目に希釈混合水溶液を添加した３ポットのいずれの苗に
も花芽が確認された。しかしながら、対照苗の３ポット
のセントポーリアにはいずれも花芽は確認されなかっ
た。更に栽培を継続したところ、希釈混合水溶液を添加
した苗の花芽は３ポット共成長を続け、５５日目には開
花株が得られたが、３ポットのコントロール苗のいずれ
にもそのような兆候は認められなかった。この結果から
明らかなように、桂皮酸／炭酸カリウム希釈混合水溶液
によりセントポーリアの開花が促進されることが確認さ
れた。結果を表１にまとめて示す。(2) Test for promoting flowering of Saintpaulia [0019] A seedling of Saintpaulia (North Carolina) grown in an artificial weather device is replanted in a small bowl (diameter: 11 cm, depth: 8 cm), liquid fertilizer is added, and the temperature is set to 2 ° C and 2 ° C. It was left for 2 weeks in an artificial weather machine under conditions of 1,000 lux and 16 hours of irradiation / day. After that, the above-mentioned diluted mixed aqueous solution of 0.0005% pure cinnamic acid was poured into a saucer (3 pots) in a pot, and the saucer was cultivated while replenishing the solution so that the solution was always filled. The case where water was used instead of the diluted mixed aqueous solution was simultaneously cultivated as a control. On the 26th day of cultivation, flower buds were confirmed in all seedlings in 3 pots to which the diluted mixed aqueous solution was added. However, flower buds were not found in any of the three pots of control seedlings of Saintpaulia. When the cultivation was further continued, the flower buds of the seedlings to which the diluted mixed aqueous solution was added continued to co-grow in 3 pots, and flowering strains were obtained on the 55th day. Was not recognized. As is clear from this result, it was confirmed that the cinnamic acid / potassium carbonate diluted mixed aqueous solution promotes flowering of Saintpaulia. The results are summarized in Table 1.
【００２０】 [0020]
【００２１】実施例２
（１）高濃度ｐ−クマル酸水溶液の製造
桂皮酸をｐ−クマル酸に代える以外は実施例１と同様に
してｐ−クマル酸の濃度が１０％であるｐ−クマル酸／
炭酸カリウム混合水溶液を製造した。これを蒸留水で２
０，０００倍に希釈し、ｐ−クマル酸の濃度が０．００
０５％であるｐ−クマル酸／炭酸カリウム希釈混合水溶
液を得た。
（２）高濃度コーヒー酸水溶液の製造
桂皮酸をコーヒー酸に代える以外は実施例１と同様にし
てコーヒー酸の濃度が１０％であるコーヒー酸／炭酸カ
リウム混合水溶液を製造した。これを蒸留水で２０，０
００倍に希釈しコーヒー酸の濃度が０．０００５％であ
るコーヒー酸／炭酸カリウム希釈混合水溶液を得た。Example 2 (1) Preparation of High Concentration p-Coumaric Acid Aqueous Solution p-Cumaric acid having a p-coumaric acid concentration of 10% was prepared in the same manner as in Example 1 except that cinnamic acid was replaced with p-coumaric acid. acid/
A potassium carbonate mixed aqueous solution was produced. 2 with distilled water
Dilute 10,000 times to obtain a p-coumaric acid concentration of 0.00
A 05% p-coumaric acid / potassium carbonate diluted mixed aqueous solution was obtained. (2) Production of High Concentration Caffeic Acid Aqueous Solution A caffeic acid / potassium carbonate mixed aqueous solution having a caffeic acid concentration of 10% was produced in the same manner as in Example 1 except that cinnamic acid was replaced with caffeic acid. This is distilled water with 20,0
A caffeic acid / potassium carbonate diluted mixed aqueous solution having a caffeic acid concentration of 0.0005% was obtained by diluting the caffeic acid by a factor of 00.
【００２２】（３）セントポーリア開花促進試験
セントポーリア（スージー）の苗を小鉢（直径：１０ｃ
ｍ、深さ：７ｃｍ）に植え付け、セントポーリア用温室
内（約２０℃、自然光）で２週間栽培した。その後受皿
に小鉢を取り付け、実施例１の桂皮酸／炭酸カリウム希
釈混合水溶液および上記の２種の希釈混合水溶液を試験
液（表２に記載）として、それぞれを各受皿（３ポッ
ト）に流し込み、試験液が常に満ちているように適宜供
給した。試験開始から２８日後に各株の花芽の形成を観
察した。結果を表２に示す。表２の結果から、桂皮酸な
どのリグニン生合成経路中間物質の希釈高濃度混合水溶
液が添加されたセントポーリア株にのみ花芽が確認さ
れ、これらの希釈高濃度混合水溶液が明らかにセントポ
ーリアの花芽形成促進に効果を有することが確認され
た。(3) St.paulia flowering promotion test St.paulia (Suzie) seedlings in small bowls (diameter: 10 c
m, depth: 7 cm) and planted in a greenhouse for Saintpaulia (about 20 ° C., natural light) for 2 weeks. Then, a small bowl was attached to the saucer, and the cinnamic acid / potassium carbonate diluted mixed aqueous solution of Example 1 and the above-described two diluted mixed aqueous solutions were used as test solutions (described in Table 2), and each was poured into each saucer (3 pots), The test solution was supplied appropriately so that it was always filled. The formation of flower buds of each strain was observed 28 days after the start of the test. The results are shown in Table 2. From the results in Table 2, flower buds were confirmed only in the Saintpaulia strain to which a diluted high-concentration mixed aqueous solution of a lignin biosynthetic pathway intermediate such as cinnamic acid was added, and these diluted high-concentration mixed aqueous solutions clearly promote the formation of flower buds in Saintpaulia. It was confirmed to have an effect on.
【００２３】 [0023]
【００２４】実施例３
（１）高濃度桂皮酸水溶液の製造
２０℃にて蒸留水４００ｍｌにトリポリリン酸ナトリウ
ム５０ｇを溶解し、これに桂皮酸１７．５ｇを加えて攪
拌、溶解した。蒸留水にて全量を５００ｍｌとし、桂皮
酸純分３．５％の桂皮酸／トリポリリン酸ナトリウム混
合水溶液を得た。次に、この混合水溶液を蒸留水にて７
００倍に希釈し、桂皮酸純分０．００５％の桂皮酸／ト
リポリリン酸ナトリウム希釈混合水溶液を得た。この希
釈混合水溶液のｐＨは８．３であった。Example 3 (1) Preparation of high-concentration aqueous cinnamic acid solution At 20 ° C., 50 g of sodium tripolyphosphate was dissolved in 400 ml of distilled water, and 17.5 g of cinnamic acid was added and stirred to dissolve. The total amount was adjusted to 500 ml with distilled water to obtain a cinnamic acid / sodium tripolyphosphate mixed aqueous solution having a cinnamic acid pure content of 3.5%. Next, this mixed aqueous solution is diluted with distilled water to 7
It was diluted to 00 times to obtain a cinnamic acid / sodium tripolyphosphate dilute mixed aqueous solution having a cinnamic acid purity of 0.005%. The pH of this diluted mixed aqueous solution was 8.3.
【００２５】（２）セントポーリア開花促進試験
６０日齢のセントポーリア（タカコ）の苗、１５０鉢を
セントポーリア用温室内の開花用ベンチに置き、１週間
後に上記の桂皮酸純分０．００５％の希釈混合水溶液を
１鉢当り５０ｍｌ散布し、栽培を続けた。対照区として
通常の水やりのみの苗を１５０鉢用意し、桂皮酸純分
０．００５％の希釈混合水溶液添加区と同様にして栽培
を続けた。２つの区のセントポーリア鉢について、出荷
に適当となる開花状態（３分咲き）になるまでの栽培日
数を比較した。この結果を表３に示す。表３の結果より
桂皮酸純分０．００５％の希釈混合水溶液はセントポー
リア（タカコ）の開花促進に有効であることが明らかに
なった。(2) St. Paulia Flowering Promotion Test 150 seedlings of 60-day-old St. Paulia (Takako) seedlings were placed on a flowering bench in a greenhouse for St. Paulia, and one week later, the above-mentioned cinnamic acid net content of 0.005% was diluted. 50 ml of the mixed aqueous solution was sprayed per pot, and the cultivation was continued. As a control group, 150 ordinary water-only seedlings were prepared, and cultivation was continued in the same manner as in the group to which a dilute mixed aqueous solution containing 0.005% of cinnamic acid was added. For the Saintpaulia pots of the two plots, the number of cultivation days until the flowering state (blooming for 3 minutes) suitable for shipping was compared. The results are shown in Table 3. From the results of Table 3, it was revealed that the diluted mixed aqueous solution having a cinnamic acid purity of 0.005% is effective for promoting flowering of Saintpaulia (Takako).
【００２６】
１）苗を開花用ベンチに置いてから出荷（３分咲き）ま
での栽培日数[0026] 1) Number of cultivation days from placing the seedlings on the flowering bench to shipping (blooming for 3 minutes)
【００２７】実施例４
（１）高濃度桂皮酸水溶液の製造
実施例１と同様にして桂皮酸純分１０％の桂皮酸／炭酸
カリウム混合水溶液を作製し、これを蒸留水にて２００
倍および２，０００倍に希釈して、桂皮酸純分濃度０．
０５％および０．００５％の希釈混合水溶液を作製し
た。
（２）小菊の害虫忌避効果確認試験
農家より入手の出荷前の小菊を４０ｃｍの丈に揃え、上
記の希釈液１７０ｍｌを入れた花瓶（高さ：２１ｃｍ、
直径：６ｃｍ、容積：２００ｍｌ）に挿した。２１℃の
室内に１４日間放置後、この小菊の表面へのアブラムシ
の着生を観察したところ表４に示す結果を得た。この結
果により、桂皮酸濃度０．０５％の希釈混合水溶液の小
菊上のアブラムシ忌避効果が明らかになった。Example 4 (1) Production of high-concentration cinnamic acid aqueous solution In the same manner as in Example 1, a cinnamic acid / potassium carbonate mixed aqueous solution having a cinnamic acid pure content of 10% was prepared, and this was diluted with distilled water to give 200%.
And 2,000 times diluted to give a cinnamic acid pure concentration of 0.
A 05% and 0.005% diluted mixed aqueous solution was prepared. (2) Test for confirming Kogiku's pest repellent effect Before shipment, Kogiku obtained from a farmer is arranged in a length of 40 cm, and a vase containing 170 ml of the above dilution liquid (height: 21 cm,
(Diameter: 6 cm, volume: 200 ml). After standing in a room at 21 ° C. for 14 days, the growth of aphids on the surface of Kogiku was observed, and the results shown in Table 4 were obtained. From these results, the aphid repellent effect on Kogiku of the dilute mixed aqueous solution having a cinnamic acid concentration of 0.05% was clarified.
【００２８】 [0028]
【００２９】実施例５
（１）高濃度桂皮酸水溶液の製造
２０℃にて蒸留水４００ｍｌに水酸化カリウム１８．４
ｇを溶解し、これに桂皮酸５０ｇを加えて攪拌、溶解
し、蒸留水にて全量を５００ｍｌとし、桂皮酸純分１０
％の桂皮酸／水酸化カリウム混合水溶液（ｐＨ８．８）
を作製した。次に、この混合水溶液を蒸留水にて２０倍
及び２００倍に希釈し、桂皮酸純分濃度０．５％および
０．０５％の希釈混合水溶液を作製した。
（２）ブーゲンビレアの害虫忌避効果確認試験
２０℃の温室内で栽培中のアブラムシの着生したブーゲ
ンビレアの植物体全体に、上記０．５％および０．０５
％の桂皮酸／水酸化カリウム希釈混合水溶液ならびに桂
皮酸飽和水溶液（約０．０５％）を霧吹きにて散布
（０．００３ｍｌ／葉ｃｍ2 ）し、１時間放置後、この
ブーゲンビレアの表面のアブラムシの着生を観察したと
ころ表５に示す結果を得た。この結果により、桂皮酸飽
和溶液より濃厚な、桂皮酸純分０．５％の希釈液を散布
した時のブーゲンビレア上のアブラムシ忌避効果が明ら
かになった。Example 5 (1) Preparation of high-concentration cinnamic acid aqueous solution 400 ml of distilled water at 20 ° C. 18.4 potassium hydroxide
Dissolve g, add 50 g of cinnamic acid to this, stir to dissolve, and bring the total volume to 500 ml with distilled water.
% Cinnamic acid / potassium hydroxide mixed aqueous solution (pH 8.8)
Was produced. Next, this mixed aqueous solution was diluted 20 times and 200 times with distilled water to prepare diluted mixed aqueous solutions having cinnamic acid pure content concentrations of 0.5% and 0.05%. (2) Pest repellent effect confirmation test of bougainvillea The above 0.5% and 0.05% were added to the whole plant of bougainvillea on which aphids were grown in a greenhouse at 20 ° C.
% Dilute cinnamic acid / potassium hydroxide diluted aqueous solution and cinnamic acid saturated aqueous solution (about 0.05%) by spraying (0.003 ml / leaf cm 2 ), and after leaving it for 1 hour, the aphid on the surface of this bougainvillea The results shown in Table 5 were obtained as a result of observing the settlement. From these results, the aphid repellent effect on bougainvillea was clarified when a diluted solution having a cinnamic acid pure content of 0.5%, which was more concentrated than the cinnamic acid saturated solution, was sprayed.
【００３０】 [0030]
【００３１】実施例６
２０℃の温室内で栽培中のアブラムシの着生したデージ
ーの植物体全体に、実施例５の桂皮酸純分０．５％およ
び０．０５％の希釈桂皮酸／水酸化カリウム希釈混合水
溶液および桂皮酸飽和水溶液を霧吹きにて散布（０．０
０３ｍｌ／葉ｃｍ2）し、１時間放置後、このデージー
の表面のアブラムシの着生を観察したところ表６に示す
結果を得た。この結果から、桂皮酸飽和溶液より濃厚な
桂皮酸濃度０．５％の希釈液を散布した時のデージー上
のアブラムシ忌避効果が明らかになった。Example 6 The whole daisy plant on which aphids had grown was grown in a greenhouse at 20 ° C., to which 0.5% cinnamic acid of Example 5 and 0.05% diluted cinnamic acid / water were added. Spray a diluted mixed solution of potassium oxide and a saturated aqueous solution of cinnamic acid by spraying (0.0
(03 ml / leaf cm 2 ) and allowed to stand for 1 hour, the aphid colonization on the surface of this daisy was observed, and the results shown in Table 6 were obtained. From this result, the aphid repellent effect on the daisy when the diluted solution having a cinnamic acid concentration of 0.5%, which is more concentrated than the cinnamic acid saturated solution, was sprayed was clarified.
【００３２】 [0032]
【００３３】実施例７
２０℃の温室内で栽培中のアブラムシの着生したクリサ
ンセマムムルチコーレの植物体全体に、実施例５の桂皮
酸純分０．５％および０．０５％の桂皮酸純分０．５％
及び０．０５％の希釈桂皮酸／水酸化カリウム希釈混合
水溶液を霧吹きにて散布（０．００３ｍｌ／葉ｃｍ2）
し、１時間放置後、このクリサンセマムムルチコーレ表
面のアブラムシの着生を観察したところ表７に示す結果
を得た。この結果から、桂皮酸飽和溶液より濃厚な桂皮
酸濃度０．５％の希釈混合水溶液を散布した時のクリサ
ンセマムムルチコーレ上のアブラムシ忌避効果が明らか
になった。Example 7 The whole plant of Chrysanthema multicore with aphids grown in a greenhouse at 20 ° C. was treated with 0.5% of cinnamic acid and 0.5% of cinnamic acid of Example 5. 0.5%
And 0.05% diluted cinnamic acid / potassium hydroxide diluted mixed aqueous solution by spraying (0.003 ml / leaf cm 2 ).
Then, after standing for 1 hour, the growth of aphids on the surface of this chrysanthemum muricole was observed, and the results shown in Table 7 were obtained. From these results, the aphid repellent effect on chrysanthemum murchole was clarified when a diluted mixed aqueous solution having a cinnamic acid concentration of 0.5%, which was more concentrated than the cinnamic acid saturated solution, was sprayed.
【００３４】 [0034]
【００３５】実施例８
２５℃の組立式室内用アルミ温室（４４０×８４０×
１，５００ｍｍ）内にアブラムシを着生させたフェリー
チェマル（鉢花）の鉢（直径１２ｃｍ）を置き、７日間
放置後、この鉢の周辺に、実施例４の桂皮酸／炭酸カリ
ウム希釈混合水溶液の入った花瓶（高さ：２１ｃｍ、直
径：６ｃｍ、容積：５００ｍｌ）に挿した大輪菊（丈:
約５０ｃｍ、径: 約７ｃｍ）を配置した。そのまま温室
内に１４日間放置後、この大輪菊の表面へのアブラムシ
の着生を観察したところ表８に示す結果を得た。この結
果から、桂皮酸純分０．０５％の希釈混合水溶液の大輪
菊上のアブラムシ忌避効果が明らかになった。Example 8 Prefabricated indoor aluminum greenhouse at 25 ° C. (440 × 840 ×
A pot (12 cm in diameter) of Felice chemal (potted flower) on which aphids were grown was placed in 1,500 mm) and left for 7 days, and then the cinnamic acid / potassium carbonate diluted mixed aqueous solution of Example 4 was placed around the pot. Large flower chrysanthemum (length: 21 cm, diameter: 6 cm, volume: 500 ml) containing
(About 50 cm, diameter: about 7 cm). After leaving it for 14 days in a greenhouse as it is, the aphid colonization on the surface of this large-flowered chrysanthemum was observed, and the results shown in Table 8 were obtained. From this result, the aphid repellent effect on the large-flowered chrysanthemum of the dilute mixed aqueous solution containing 0.05% of cinnamic acid was clarified.
【００３６】 [0036]
【００３７】実施例９
（１）高濃度桂皮酸溶液の製造
２０℃にて蒸留水４００ｍｌに炭酸カリウム５０ｇを溶
解し、これに桂皮酸５０ｇを加えて攪拌、溶解した。蒸
留水にて全量を５００ｍｌとし、桂皮酸純分１０％の桂
皮酸／炭酸カリウム混合水溶液を得た。次に、この混合
水溶液を蒸留水にて２，０００倍に希釈し、桂皮酸純分
０．００５％の桂皮酸／炭酸カリウム混合水溶液の希釈
液を得た。この希釈液のｐＨは８．９であった。Example 9 (1) Preparation of high-concentration cinnamic acid solution At 20 ° C., 50 g of potassium carbonate was dissolved in 400 ml of distilled water, to which 50 g of cinnamic acid was added and stirred to dissolve. The total amount was adjusted to 500 ml with distilled water to obtain a cinnamic acid / potassium carbonate mixed aqueous solution having a cinnamic acid pure content of 10%. Next, this mixed aqueous solution was diluted 2000 times with distilled water to obtain a diluted solution of a cinnamic acid / potassium carbonate mixed aqueous solution having a cinnamic acid pure content of 0.005%. The pH of this diluted solution was 8.9.
【００３８】（２）洋蘭（ファレノプシスギャラクシ
ー）の開花促進試験
同一日齢の洋蘭（ファレノプシスギャラクシー）の苗１
０鉢を購入し、２５℃の組立式室内用アルミ温室（４４
０×８４０×１，５００ｍｍ）内に置いた。１０鉢の内
５鉢を桂皮酸／炭酸カリウム希釈混合水溶液添加区と
し、１鉢当り２０ｍｌの桂皮酸純分が０．００５％の桂
皮酸／炭酸カリウム希釈混合水溶液を４日置きに７回添
加し、栽培を続けた。残りの５鉢は対照区として上記桂
皮酸／炭酸カリウム希釈混合水溶液の代わりに同量の水
を添加し、桂皮酸／炭酸カリウム希釈混合水溶液添加区
と同様にして栽培を続けた。２つの区のファレノプシス
ギャラクシー鉢について、最初に桂皮酸純分が０．００
５％の桂皮酸／炭酸カリウム希釈混合水溶液を添加した
日から数えて２５日目まで花の数を比較した。この結果
を表９に示す。表９の結果より桂皮酸／炭酸カリウム希
釈混合水溶液はファレノプシスギャラクシーの開花促進
に有効であることが明らかになった。(2) Flowering promotion test of Western orchid (Phallenopsis galaxy) Seedling 1 of Western orchid (Phalenopsis galaxy) of the same age
Purchased 0 pots, and a 25 ℃ prefabricated indoor aluminum greenhouse (44
(0 × 840 × 1,500 mm). 5 pots out of 10 pots were set as a cinnamic acid / potassium carbonate diluted mixed aqueous solution addition zone, and 20 ml of cinnamic acid / 0.005% pure cinnamic acid / potassium carbonate diluted mixed aqueous solution was added 7 times every 4 days. And continued to grow. In the remaining 5 pots, as a control group, the same amount of water was added instead of the above-mentioned cinnamic acid / potassium carbonate diluted mixed aqueous solution, and cultivation was continued in the same manner as in the cinnamic acid / potassium carbonate diluted mixed aqueous solution added group. For the Phalaenopsis Galaxy pots of the two plots, the first content of cinnamic acid was 0.00
The numbers of flowers were compared from the day when the 5% cinnamic acid / potassium carbonate diluted mixed aqueous solution was added to the 25th day. The results are shown in Table 9. From the results in Table 9, it was revealed that the cinnamic acid / potassium carbonate diluted mixed aqueous solution is effective for promoting flowering of Phalaenopsis galaxy.
【００３９】 [0039]
【００４０】実施例１０
（１）高濃度桂皮酸溶液の製造
２０℃にて蒸留水４００ｍｌに水酸化カリウム１８．４
ｇを溶解し、これに桂皮酸５０ｇを加えて攪拌、溶解
し、蒸留水にて全量を５００ｍｌとし、桂皮酸純分１０
％の桂皮酸／水酸化カリウム混合水溶液（ｐＨ８．８）
を得た。次に、この混合水溶液を蒸留水にて１，０００
倍に希釈し、桂皮酸純分０．０１％の桂皮酸／水酸化カ
リウム希釈混合水溶液を得た。Example 10 (1) Production of high-concentration cinnamic acid solution At 20 ° C., 400 ml of distilled water was added with 18.4 potassium hydroxide.
Dissolve g, add 50 g of cinnamic acid to this, stir to dissolve, and bring the total volume to 500 ml with distilled water.
% Cinnamic acid / potassium hydroxide mixed aqueous solution (pH 8.8)
Got Next, this mixed aqueous solution is diluted with distilled water to 1,000
Diluted twice to obtain a cinnamic acid / potassium hydroxide diluted mixed aqueous solution having a cinnamic acid pure content of 0.01%.
【００４１】（２）ペチュニアの開花促進試験
２９日齢で鉢上げしたペチュニアの苗２５鉢に、鉢上げ
後１６日目と３０日目に１鉢当り５０ｍｌの上記の桂皮
酸純分０．０１％の桂皮酸／水酸化カリウム希釈混合水
溶液を散布し、栽培を続けた。対照区として通常の水や
りのみの苗を２５鉢用意し、桂皮酸／水酸化カリウム希
釈混合水溶液添加区と同様にして栽培を続けた。２つの
区のペチュニア鉢について、出荷に適当となる開花状態
（３分咲き）になるまでの栽培日数を比較した。この結
果を表１０に示す。表１０の結果より桂皮酸純分０．０
１％の桂皮酸／水酸化カリウム希釈混合水溶液はペチュ
ニアの開花促進に有効であることが明らかになった。(2) Petunia Flower Acceleration Test On 25 pots of petunia seedlings potted at 29 days of age, 50 ml of the above cinnamic acid pure contents of 0.01 per pot was placed on 25th pots of petunia seeds 16 and 30 days after potting. % Cinnamic acid / potassium hydroxide diluted mixed aqueous solution was sprayed, and cultivation was continued. Twenty-five ordinary seedlings only watered were prepared as a control group, and cultivation was continued in the same manner as in the group to which a cinnamic acid / potassium hydroxide diluted mixed aqueous solution was added. With respect to the petunia pots of the two plots, the cultivation days until the flowering state (blooming for 3 minutes) suitable for shipment was compared. The results are shown in Table 10. From the results of Table 10, cinnamic acid pure content 0.0
It was revealed that a 1% cinnamic acid / potassium hydroxide diluted mixed aqueous solution is effective for promoting flowering of petunia.
【００４２】 [0042]
【００４３】実施例１１
（１）高濃度桂皮酸溶液の製造
実施例９と同様にして桂皮酸純分１０％の桂皮酸／炭酸
カリウム混合水溶液を調製し、これを蒸留水にて２，０
００倍に希釈し、桂皮酸純分０．００５％の桂皮酸／炭
酸カリウム希釈混合水溶液を得た。この希釈液のｐＨは
８．９であった。
（２）シクラメンの開花促進試験
播種から３５６日を経たシクラメンの苗を５号鉢５鉢に
定植し、１鉢当り５０ｍｌ桂皮酸／炭酸カリウム希釈混
合水溶液を１５日おきに３回散布し、栽培を続けた。対
照区として通常の水やりのみの苗を５鉢用意し、桂皮酸
／炭酸カリウム希釈混合水溶液添加区と同様にして栽培
を続けた。２つの区のシクラメン鉢について、出荷に適
当となる開花状態（３分咲き）になるまでの栽培日数を
比較した。この結果を表１１に示す。表１１の結果より
桂皮酸純分０．００５％の桂皮酸／炭酸カリウム希釈混
合水溶液はシクラメンの開花促進に有効であることが明
らかになった。Example 11 (1) Production of high-concentration cinnamic acid solution In the same manner as in Example 9, a cinnamic acid / potassium carbonate mixed aqueous solution having a cinnamic acid purity of 10% was prepared, and this was diluted with distilled water to give 2,0.
It was diluted to 100 times to obtain a cinnamic acid / potassium carbonate diluted mixed aqueous solution having a cinnamic acid pure content of 0.005%. The pH of this diluted solution was 8.9. (2) Cyclamen flowering promotion test Planting cyclamen seedlings 356 days after sowing in No. 5 pots and 5 pots, spraying 50 ml cinnamic acid / potassium carbonate diluted mixed aqueous solution 3 times every 15 days for cultivation. Continued. As a control group, 5 ordinary water-only seedlings were prepared, and cultivation was continued in the same manner as in the group to which the cinnamic acid / potassium carbonate diluted mixed aqueous solution was added. With respect to the cyclamen pots of the two plots, the number of cultivation days until the flowering state (blooming for 3 minutes) suitable for shipping was compared. The results are shown in Table 11. From the results in Table 11, it was revealed that a cinnamic acid / potassium carbonate diluted mixed aqueous solution having a cinnamic acid pure content of 0.005% is effective for promoting flowering of cyclamen.
【００４４】 [0044]
【００４５】実施例１２
（１）高濃度桂皮酸溶液の製造
実施例１１と同様にして、桂皮酸純分０．００５％の桂
皮酸／炭酸カリウム希釈混合水溶液を作製した。この希
釈液のｐＨは８．９であった。
（２）ユリの開花促進試験
５号鉢５鉢にテッポウユリの球根を植え付け、１５１日
齢の時に１鉢当り５０ｍｌ桂皮酸／炭酸カリウム希釈混
合水溶液を１５日おきに３回散布して栽培を続けた。対
照区として通常の水やりのみの苗を５鉢用意し、桂皮酸
／炭酸カリウム希釈混合水溶液添加区と同様にして栽培
を続けた。２つの区のテッポウユリ鉢について、３分咲
きの開花状態になるまでの栽培日数を比較した。この結
果を表１２に示す。表１２の結果より桂皮酸純分０．０
０５％の桂皮酸／炭酸カリウム希釈混合水溶液はテッポ
ウユリの開花促進に有効であることが明らかになった。Example 12 (1) Production of high-concentration cinnamic acid solution In the same manner as in Example 11, a cinnamic acid / potassium carbonate diluted mixed aqueous solution having a cinnamic acid purity of 0.005% was prepared. The pH of this diluted solution was 8.9. (2) Lily flowering promotion test Planting 5 lily bulbs with Teppo lily bulbs, at 151 days of age, 50 ml cinnamic acid / potassium carbonate diluted mixed aqueous solution was sprayed 3 times every 15 days to continue cultivation. It was As a control group, 5 ordinary water-only seedlings were prepared, and cultivation was continued in the same manner as in the group to which the cinnamic acid / potassium carbonate diluted mixed aqueous solution was added. The number of cultivation days until the flowering state of three-minute blooming was compared for the teppo lily pots of the two plots. The results are shown in Table 12. From the results of Table 12, the cinnamic acid pure content 0.0
It was revealed that a 05% cinnamic acid / potassium carbonate diluted mixed aqueous solution is effective for promoting flowering of the teppo lily.
【００４６】 [0046]
【００４７】実施例１３
（１）高濃度桂皮酸水溶液の製造
実施例１０と同様にして桂皮酸純分０．０１％の桂皮酸
／水酸化カリウム希釈混合水溶液を作製した。
（２）ブロッコリーの花蕾促成試験
パイプハウス（ビニルハウス）内にブロッコリーの苗を
植えた大型ポット（直径：３０ｃｍ、高さ：２５ｃｍ）
を置き、栽培を開始した。その後、栽培７０日目及び８
４日目に、１ポット当り１００ｍｌの上記希釈混合水溶
液を如雨露にて植物体に散布した。栽培開始から１１４
日目に花蕾（１ポットに１つ成育）の大きさを測定し、
上記希釈混合水溶液を散布しない対象の花蕾の大きさと
比較した。この結果を表１３に示す。表１３の結果から
上記希釈混合水溶液を散布したブロッコリーの花蕾は、
対象の花蕾に比べて、直径で１９．７％大きく、桂皮酸
純分０．０１％の桂皮酸／水酸化カリウム希釈混合水溶
液はブロッコリーの花蕾促成効果を有することが分かっ
た。Example 13 (1) Production of high-concentration cinnamic acid aqueous solution In the same manner as in Example 10, a cinnamic acid / potassium hydroxide diluted mixed aqueous solution having a cinnamic acid pure content of 0.01% was prepared. (2) Large-scale pot (diameter: 30 cm, height: 25 cm) with broccoli seedlings planted in a broccoli flower bud forcing test pipe house (vinyl house)
Was placed and cultivation was started. After that, 70th day of cultivation and 8th
On the 4th day, 100 ml of the above diluted mixed aqueous solution per pot was sprayed on the plant body by rainy dew. 114 from the start of cultivation
On the day, measure the size of the flower bud (one grown in one pot),
The size was compared with the size of the target flower bud which was not sprayed with the diluted mixed aqueous solution. The results are shown in Table 13. From the results of Table 13, the florets of broccoli sprayed with the diluted mixed aqueous solution are:
It was found that a cinnamic acid / potassium hydroxide diluted mixed aqueous solution having a cinnamic acid pure content of 0.01% was 19.7% larger in diameter than the target flower bud and had a broccoli flower bud promoting effect.
【００４８】 [0048]
【００４９】実施例１４
（１）高濃度桂皮酸水溶液の製造
実施例１０と同様にして桂皮酸純分０．０１％の桂皮酸
／水酸化カリウム希釈混合水溶液を作製した。
（２）カリフラワーの花蕾促成試験
パイプハウス（ビニルハウス）内にカリフラワーの苗を
植えた大型ポット（直径：３０ｃｍ、高さ：２５ｃｍ）
を置き、栽培を開始した。その後、栽培７０日目、８４
日目及び１００日目に、１ポット当り１００ｍｌの上記
希釈混合水溶液を如雨露にて植物体に散布した。栽培開
始から１１５日目に花蕾（１ポットに１つ成育）の大き
さを測定し、上記希釈混合水溶液を散布しない対象の花
蕾の大きさと比較した。この結果を表１４に示す。表１
４の結果から上記混合水溶液希釈液を散布したブロッコ
リーの花蕾は、対象の花蕾に比べて、直径で２３．５％
大きく、桂皮酸純分０．０１％の桂皮酸／水酸化カリウ
ム希釈混合水溶液はカリフラワーの花蕾促成効果を有す
ることが分かった。Example 14 (1) Production of high-concentration cinnamic acid aqueous solution In the same manner as in Example 10, a cinnamic acid / potassium hydroxide diluted mixed aqueous solution having a cinnamic acid pure content of 0.01% was prepared. (2) Cauliflower flower bud forcing test Large pot (diameter: 30 cm, height: 25 cm) with cauliflower seedlings planted in a pipe house (vinyl house)
Was placed and cultivation was started. After that, 70th day of cultivation, 84
On the 1st and 100th days, 100 ml of the above diluted mixed aqueous solution per pot was sprayed on the plant body by rainy dew. On the 115th day from the start of cultivation, the size of the flower bud (one grown in one pot) was measured and compared with the size of the target flower bud to which the above diluted mixed aqueous solution was not sprayed. The results are shown in Table 14. Table 1
From the result of 4, the broccoli flower buds sprayed with the diluted solution of the mixed aqueous solution are 23.5% in diameter as compared with the target flower buds.
It was found that a cinnamic acid / potassium hydroxide diluted mixed aqueous solution having a cinnamic acid pure content of 0.01% had a cauliflower flower bud promoting effect.
【００５０】 [0050]
【００５１】実施例１５
実施例２のコーヒー酸およびｐ−クマル酸の混合希釈水
溶液を用いてセントポーリア（実施例２）以外の他の実
施例における花卉および花卉野菜について開花促進効
果、花蕾促成効果およびアブラムシ忌避効果を確認した
が、桂皮酸の混合希釈水溶液を用いた場合と同様の結果
が得られた。Example 15 Using the mixed dilute aqueous solution of caffeic acid and p-coumaric acid of Example 2, flowering and flower vegetation promoting effects, flower bud promoting effects and flowering florets and flower vegetative vegetables in other Examples other than Saintpaulia (Example 2) Although the aphid repellent effect was confirmed, the same results as when using a mixed dilute aqueous solution of cinnamic acid were obtained.
【００５２】[0052]
【発明の効果】以上の本発明によれば桂皮酸等のリグニ
ン生合成経路の中間物質を、その水に対する最大溶解度
（室温）以上の高濃度に水に溶解した水溶液タイプの農
・園芸用の生理活性剤が提供される。本発明の生理活性
剤は、農・園芸用植物の開花促進剤、花蕾促成剤、害虫
忌避剤、防黴剤等として有用である。INDUSTRIAL APPLICABILITY According to the present invention as described above, an intermediate substance of the lignin biosynthetic pathway such as cinnamic acid is dissolved in water at a high concentration of at least the maximum solubility (room temperature) in water and used for agricultural and horticultural purposes. A bioactive agent is provided. The bioactive agent of the present invention is useful as a flowering promoter, a flower bud promoting agent, a pest repellent, a fungicide, etc. for agricultural and horticultural plants.
───────────────────────────────────────────────────── フロントページの続き Ｆターム(参考） 2B022 AB17 EA01 2B121 AA11 CC34 EA30 FA16 4H011 AA01 AA03 AC06 BA01 BA04 BB06 BC18 DA13 DD03 DG04 DG06 ─────────────────────────────────────────────────── ─── Continued front page F term (reference) 2B022 AB17 EA01 2B121 AA11 CC34 EA30 FA16 4H011 AA01 AA03 AC06 BA01 BA04 BB06 BC18 DA13 DD03 DG04 DG06
Claims (7)
その水に対する最大溶解度を超える濃度に水に溶解して
なることを特徴とする農・園芸用生理活性剤。1. A cinnamic acid and / or cinnamic acid derivative,
A bioactive agent for agriculture and horticulture, which is characterized by being dissolved in water at a concentration exceeding the maximum solubility in water.
ヒー酸である請求項１に記載の農・園芸用生理活性剤。2. The bioactive agent for agricultural and horticultural use according to claim 1, wherein the cinnamic acid derivative is p-coumaric acid or caffeic acid.
記載の農・園芸用生理活性剤。3. The agricultural / horticultural bioactive agent according to claim 1, which contains a solubilizing agent which is soluble in water.
する無機物質である請求項３に記載の農・園芸用生理活
性剤。4. The bioactive agent for agricultural and horticultural use according to claim 3, wherein the solubilizing agent is an inorganic substance that is alkaline when dissolved in water.
水酸化カリウム及び炭酸カリウムから選択される少なく
とも１種である請求項４に記載の農・園芸用生理活性
剤。5. The dissolution aid is sodium tripolyphosphate,
The agricultural / horticultural bioactive agent according to claim 4, which is at least one selected from potassium hydroxide and potassium carbonate.
記載の農・園芸用生理活性剤。6. The agricultural / horticultural bioactive agent according to claim 1, which has a concentration of 25% by weight or less.
抗菌剤または防黴剤である請求項１〜６のいずれか１項
に記載の農・園芸用生理活性剤。7. A flowering promoting agent, a flower bud promoting agent, a pest repellent,
The agricultural / horticultural bioactive agent according to any one of claims 1 to 6, which is an antibacterial agent or an antifungal agent.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002036577A JP4256104B2 (en) | 2002-02-14 | 2002-02-14 | Aphid repellent for flower buds or flower vegetables |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002036577A JP4256104B2 (en) | 2002-02-14 | 2002-02-14 | Aphid repellent for flower buds or flower vegetables |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2003235357A true JP2003235357A (en) | 2003-08-26 |
| JP4256104B2 JP4256104B2 (en) | 2009-04-22 |
Family
ID=27778429
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002036577A Expired - Fee Related JP4256104B2 (en) | 2002-02-14 | 2002-02-14 | Aphid repellent for flower buds or flower vegetables |
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| JP (1) | JP4256104B2 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011190178A (en) * | 2010-03-11 | 2011-09-29 | Dainichiseika Color & Chem Mfg Co Ltd | Phytopathogenic fungus inhibitor, fertilizer composition for inhibiting phytopathogenic fungus and method for producing the same |
| JP2012092029A (en) * | 2010-10-26 | 2012-05-17 | Dainichiseika Color & Chem Mfg Co Ltd | Plant growth regulating agent, fertilizer composition, and method of manufacturing the same |
| CN116602295A (en) * | 2023-06-07 | 2023-08-18 | 青岛农业大学 | Application of trans-cinnamic acid, p-coumaric acid, isoliquiritigenin or formononetin in pasture growth regulator |
| WO2024074495A1 (en) * | 2022-10-04 | 2024-04-11 | Cysbio Aps | Method to inhibit proliferation of a phytopathogen on plants and compositions used for this purpose |
-
2002
- 2002-02-14 JP JP2002036577A patent/JP4256104B2/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011190178A (en) * | 2010-03-11 | 2011-09-29 | Dainichiseika Color & Chem Mfg Co Ltd | Phytopathogenic fungus inhibitor, fertilizer composition for inhibiting phytopathogenic fungus and method for producing the same |
| JP2012092029A (en) * | 2010-10-26 | 2012-05-17 | Dainichiseika Color & Chem Mfg Co Ltd | Plant growth regulating agent, fertilizer composition, and method of manufacturing the same |
| WO2024074495A1 (en) * | 2022-10-04 | 2024-04-11 | Cysbio Aps | Method to inhibit proliferation of a phytopathogen on plants and compositions used for this purpose |
| CN116602295A (en) * | 2023-06-07 | 2023-08-18 | 青岛农业大学 | Application of trans-cinnamic acid, p-coumaric acid, isoliquiritigenin or formononetin in pasture growth regulator |
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| Publication number | Publication date |
|---|---|
| JP4256104B2 (en) | 2009-04-22 |
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