JP2003192561A - Skin cosmetic - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin cosmetic having excellent skin-moisturizing, aging- proofing and skin-beautifying effects and high safety. <P>SOLUTION: This skin cosmetic contains 0.001-20 wt.% of a β-glucan extracted from a gramineous plant, especially barley or oats, and having at least two kinds of linkages among 1-2-β-D-glucopyranose, 1-3-β-D-glucopyranose, 1,4-β-D-glucopyranose and 1-6-β-D-glucopyranose linkages and a weight-average molecular weight of ≤100,000. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a skin cosmetic, and more particularly to a skin cosmetic excellent in skin moisturizing action, anti-aging effect, beautiful skin effect and the like.

[0002]

2. Description of the Prior Art Aged skin becomes dry and rough skin without smoothness, and keratinocyte exfoliation phenomenon is observed. It is said that the aged skin has a slow turnover speed, and when the antiaging effect is developed and imparted to the skin, the turnover speed of the skin is increased. Heretofore, various cosmetics have been proposed which give a proper moisturizing feeling and flexibility to the skin surface, and in order to give a moist feeling to the skin, propylene glycol, 1,3 butylene glycol, glycerin, as a moisturizing agent in the skin cosmetics, Polyglycerin and the like have been blended, but in order to obtain the moisturizing effect, these humectants are blended in a large amount of 5% by weight or more, and there is a drawback that a sticky feeling occurs. In addition, the above skin cosmetics,
Although it acts on the epidermis of the skin tissue, it rarely acts on the dermis, which is the tissue under the epidermis, and therefore, none of them has a sufficient effect for preventing skin aging as described above. In addition, it gives the texture of the skin, firmness, and the effect of beautiful skin,
At present, there is no satisfactory product, and a highly safe product is desired when used as a skin cosmetic.

[0003]

SUMMARY OF THE INVENTION Therefore, an object of the present invention is to provide a skin cosmetic having excellent skin moisturizing effect, anti-aging effect, beautiful skin effect and the like and further high safety.

[0004]

[Means for Solving the Problems] The inventors of the present invention exerted excellent effects on skin moisturizing action, anti-aging effect, skin beautiful effect, etc. by incorporating β-glucan extracted from a grass family plant into a skin cosmetic composition. The present invention has been completed and the present invention has been completed.

That is, the present invention provides a skin cosmetic containing a β-glucan extracted from a grass family plant.

The present invention also has a 1-2-β-D-glucopyranose bond, a 1-3-β-D-glucopyranose bond, a 1-4-β-D-glucopyranose bond, and a 1-6-
It is intended to provide the skin cosmetic containing β-glucan having at least two types of β-D-glucopyranose bonds.

Further, the present invention provides the above skin cosmetic containing β-glucan having a 1-3-β-D-glucopyranose bond and a 1-4-β-D-glucopyranose bond.

The present invention also provides the above-mentioned skin cosmetic, wherein the above-mentioned grass family plant is barley or oats.

The present invention also provides the above skin cosmetic, wherein the β-glucan has a weight average molecular weight of 100,000 or less. Further, the present invention has the above β-glucan content,
The above-mentioned skin cosmetic is provided in an amount of 0.001 to 20% by weight based on the total amount of the skin cosmetic.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below.

The β-glucan extracted from the Gramineae plant used in the skin cosmetics of the present invention is not particularly limited in its extraction method, and may be extracted by adding an extraction solvent to the Gramineae plant as a raw material for extraction. Good. In addition, the extract itself in the case of solid-liquid separation, or a liquid or solid one obtained by concentrating β-glucan extracted by a known method from the extract, or purified by a known method from the extract to obtain purity. Even if it is obtained by any manufacturing method, such as raised liquid or solid one,
Any form and any purity can be used. Of course, there is no problem even if the extracted components other than β-glucan are mixed. In the present invention, all of them are referred to as β-glucan extracted from grasses (hereinafter, also referred to as extracted β-glucan).

[0012] Examples of grasses include rice, wheat,
Grains such as corn, sorghum, millet, millet, millet, barley, oats (crown) and rye can be mentioned. Preferred is barley or oats. Although the whole plant can be used as a raw material for extraction, it is preferable to use seeds having a relatively high β-glucan content. Any of the bran, bran, malt, germ, and endosperm parts obtained in the grain refining process may be used, including those obtained by crushing the whole (whole grain flour). Preferably whole grains and grains of barley and oats are rice bran and rice bran generated at the time of polishing the grain and grains from the outer peripheral portion, rice bran, wheat and corn bran and germ, and the like, more preferably barley and It is the endosperm part obtained by polishing the whole grain and grains of oats from the outer periphery and the bran generated at that part.

Further, β-glucan extracted from the Gramineae plant of the present invention includes 1-2-β-D-glucopyranose bond, 1-3-β-D-glucopyranose bond, 1-4-
β-glucan having two or more kinds of β-D-glucopyranose bond and 1-6-β-D-glucopyranose bond is preferable, and 1-3-β-D-glucopyranose bond and 1-
It is preferable to contain β-glucan composed of 4-β-D-glucopyranose bond.

Further, the method for extracting β-glucan of the present invention from grasses will be explained. The β-glucan of the present invention is a water-soluble polymer and can be dissolved as an aqueous solution. Grain powder of water, warm water,
Hot water or salt solution, further acid, alkaline aqueous solution,
Using an organic solvent or the like, it can be extracted with a solvent in an amount of 2 to 100 times the amount of the powder at any temperature for any time. Furthermore, β-glucan can be obtained by solid-liquid separation of the extract. Among these, β-glucan extracted with water, warm water or hot water is preferable, and β-glucan extracted with warm water at a temperature of 80 ° C. or lower and 4 ° C. or higher is more preferable. Further, an extraction accelerator or the like may be added at the time of extraction.

Specifically, as a method for obtaining a high molecular weight β-glucan from barley, for example, a method in which polywax barley is used as a raw material and water extraction is used (Japanese Patent Publication No. 4-11197).
Gazette), or a method of obtaining β-glucan having a weight average molecular weight of 100,000 to 1,000,000 by alkali extraction, neutralization and alcohol precipitation using barley or oat as a raw material (Japanese Patent Publication No. 6-83652), and yield of luster. A method of extracting β-glucan with hot water of 80 to 90 ° C. using barley bran of 82% or less as a raw material (JP-A-11-225706)
The β-glucan obtained by the above method, or the β-glucan obtained by the production method thereof is further reduced to a low-molecular β-glucan by a known method. For example, as a method for lowering the molecular weight, any known hydrolysis reaction of polysaccharides can be used.
For example, it is known that a water-soluble polysaccharide is hydrolyzed by heating under pressure in the presence of an acid, and this can be utilized to lower the molecular weight. In addition, it is also effective to lower the molecular weight by utilizing the hydrolysis reaction by an enzyme.
Glucanase or the like can be used. Furthermore, WO
98/13056, Japanese Patent Application No. 2000-287920.
It is also possible to use β-glucan obtained by directly extracting from raw material grains by the method described in No. Also, Japanese Patent Application 2000-2
You may use the extraction promoter etc. which are described in 95660.

The β-glucan extracted from the Gramineae plant used in the present invention is a polymer, and β-glucan having any weight average molecular weight can be used. However, the molecular weight decreases and the affinity to the skin is good. The molecular weight is preferably 3,000,000 or less, preferably 500,000 or less, and more preferably 100,000 or less because the antiaging effect is also increased. The extracted β-glucan may be reduced to a low molecular weight by a known method, or the low-molecular weight β-glucan may be directly extracted.

When the extract is not extracted and purified from the Gramineae plant and the extract is used as it is or after being subjected to only powdering and solidifying treatments, the β-glucan in the component has a purity of The higher the purity, the better, but 1
-100%, preferably 10-100%, more preferably 20-100%.

The amount of β-glucan extracted from Gramineae plants in skin cosmetics is 0.
001 to 20% by weight is preferable. If the amount is less than 0.001% by weight, the effect of the present invention is not sufficiently exhibited, and if the amount is more than 20% by weight, the effect commensurate with the increase in the amount of the compound may not be expected.

The method of blending β-glucan extracted from a grass family is not particularly limited in the form of β-glucan, and as it is, or by dissolving or emulsifying or dispersing them in water or another water-soluble solvent, It can be added to cosmetics.

The skin cosmetic of the present invention is highly safe because it uses β-glucan extracted from a grass family plant which is a natural product.

The skin cosmetic of the present invention may further contain, in addition to β-glucan extracted from the grass family, a substance which gives some physiological activity to the skin when applied to the skin. For example, whitening ingredients, anti-inflammatory agents, anti-aging agents, slimming agents, tightening agents, antioxidants, hair growth agents, hair growth agents, blood circulation promoters, moisturizing ingredients other than polyhydric alcohols or sugars, desiccants, cooling agents. , Warming agents, amino acids, wound healing promoters, stimulants, analgesics, cell activating agents, enzyme components and the like.

The above-mentioned substances which give physiological activity to the skin include physiologically active components such as a natural plant-derived component, seaweed extract component, herbal medicine component and the like, for example, acitaba extract, avocado extract, armature extract, artea extract. , Arnica extract, Aloe extract, Apricot extract, Apricot kernel extract, Ginkgo biloba extract, Ginkgo biloba extract, Turmeric extract, Oolong tea extract, Ages extract, Echinacea leaf extract, Ougon extract, Oataku extract, Oren extract, Barley extract, Hypericum extract, Odorikosou extract , Dutch mustard extract, orange extract, dried seawater, seaweed extract, hydrolyzed elastin,
Hydrolyzed wheat powder, hydrolyzed silk, chamomile extract,
Carrot extract, sagebrush extract, licorice extract, oil-soluble licorice extract, karkade extract, oyster extract, kiwi extract, kina extract, cucumber extract, guanosine, gardenia extract, kumazasa extract, clara extract, walnut extract, grapefruit extract, clematis extract, chlorella extract. , Mulberry extract, gentian extract, black tea extract, yeast extract, burdock extract, rice bran extract, rice germ oil, comfrey extract, collagen, cowberry extract, cinnamon extract, psychic extract, saitai extract, salvia extract, savon extract, sasa extract , Hawthorn extract, hawthorn extract,
Shiitake extract, dioxin extract, shikon extract, perilla extract, linden extract, sycamore extract, peony extract, ginger root extract, birch extract, horse mackerel extract, quince extract, hawthorn extract, Sambucus nigra extract, yarrow millet extract, mint extract, sage extract. , Mallow extract, senkyu extract, senburi extract, soybean extract, turmeric extract, thyme extract, tea extract, clove extract, chigaya extract, chimpi extract, touki extract, quince extract, tounin extract, spruce extract, dokudami extract, tomato extract, natto extract,
Carrot extract, garlic extract, Novara extract, Hibiscus extract, Bakmondow extract, Parsley extract,
Honey, Hamamelis extract, Parietaria extract, Hikiokoshi extract, bisabolol, loquat extract, coltsfoot extract, Fukinoto extract, Bukuro extract, Butcher bloom extract, grape extract, placenta extract, propolis, loofah extract, safflower extract, peppermint extract, bodaiju extract, button Extract, hop extract, pine extract, horse chestnut extract, sphagnum extract, sucrose extract, melissa extract, peach extract, cornflower extract, eucalyptus extract, yukinoshita extract, yuzu extract, yokuinin extract, mugwort extract, lavender extract, apple extract, lettuce extract,
Examples thereof include lemon extract, astragalus extract, rose extract, rosemary extract, Roman chamomile extract, royal jelly extract and the like.

Specific examples of preferable physiologically active ingredients such as other naturally derived ingredients include deoxyribonucleic acid, raffinose, mucopolysaccharides, hyaluronic acid or salts thereof such as sodium hyaluronate, sodium chondroitin sulfate,
Biopolymers such as collagen, elastin, chitin, chitosan and hydrolyzed eggshell membranes; amino acid, sarcosine, N-methyl-L-serine and other amino acid derivatives; ethyl glucose, sodium lactate, urea, sodium pyrrolidonecarboxylate, betaine, whey Moisturizing ingredients other than polyhydric alcohols or sugars such as; oily ingredients such as sphingolipids, ceramides, cholesterol, cholesterol derivatives, phospholipids; ε-aminocaproic acid, glycyrrhizic acid, β-glycyrrhetinic acid, lysozyme chloride, guaiazulene, hydrocortisone Anti-inflammatory agent; vitamins A, B ₂ , B ₆ ,
Vitamins such as D, calcium pantothenate, biotin, nicotinic acid amide, and vitamin E; active ingredients such as allantoin, diisopropylamine dichloroacetic acid, 4-aminomethylcyclohexanecarboxylic acid; anti-effects such as carotenoids, flavonoids, tannins, lignans, and saponins Oxidizing agents; cell activating agents such as α-hydroxy acid, β-hydroxy acid and mevalonic acid, blood circulation promoting agents such as γ-oryzanol, wound healing agents such as retinol and retinol derivatives, ascorbic acids, arbutin, kojic acid, placenta extract ,
Whitening agents such as sulfur, ellagic acid, linoleic acid, tranexamic acid and glutathione; cepharanthin, licorice extract, capsicum tincture, hinokitiol, garlic iodide extract, pyridoxine hydrochloride, nicotinic acid, nicotinic acid derivatives, calcium pantothenate, D-panto. Tenyl alcohol, acetylpantothenyl ethyl ether, biotin, allantoin, isopropyl methylphenol,
Estradiol, ethinyl esteradiol, capronium chloride, benzalkonium chloride, diphenhydramine hydrochloride, tacanal, camphor, salicylic acid, nonyl vanillylamide, nonanoic acid vanillylamide, piroctone olamine, glyceryl pentadecanoate, l-menthol, mononitroguaiacol, resorcin, γ-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormone, cantharitin tincture, cyclosporine, zinc pyrithione, hydrocortisone, minoxidil, polyoxyethylene sorbitan monostearate,
Hair-growth agents such as peppermint oil and Sasanishiki extract; β-glucans obtained from things other than gramineous plants such as microorganisms and mushrooms.

The above-mentioned ascorbic acids are ascorbic acid, ascorbic acid sulfuric acid ester, ascorbic acid phosphoric acid ester, ascorbic acid higher fatty acid ester, and salts thereof. Those salts include sodium salt, potassium salt, magnesium salt, calcium salt, barium salt, ammonium salt, monoethanolamine salt,
Examples thereof include diethanolamine salt, triethanolamine salt, monoisopropanolamine salt, diisopropanolamine salt, triisopropanolamine salt and the like. Examples of the above-mentioned ascorbic acid sulfuric acid ester include ascorbic acid-2-sulfuric acid ester and ascorbic acid-3.
-Sulfuric acid ester, and examples of ascorbic acid phosphoric acid ester include ascorbic acid-2-phosphoric acid ester and ascorbic acid-3-phosphoric acid ester, which are known substances and are disclosed in JP-B-44-31237. It is described in Japanese Patent Publication No. 54-21415.
Examples of higher fatty acid ascorbic acid ester include ascorbic acid-2-palmitic acid monoester, ascorbic acid-2,6-palmitic acid diester, and ascorbic acid-2-stearic acid ester.

The amount of the above-mentioned component that imparts some physiological activity to the skin when applied to the skin to the skin cosmetic is
It is preferably 0.05 to 90% by weight, and more preferably 0.1 to 50% by weight, based on the total amount of the skin cosmetic, depending on the effect-expressing concentration of the active ingredient.

In addition to the above-mentioned components, the skin cosmetic of the present invention includes oils, powders (pigments, dyes, resins), fluorine compounds, resins, surfactants, viscous agents, which are usually used in cosmetics.
Ingredients such as preservatives, fragrances, antibacterial agents, bactericides, salts, solvents, chelating agents, neutralizing agents, pH adjusting agents and insect repellents can be used.

As an example of the above powder, red No. 104,
Red No. 201, Yellow No. 4, Blue No. 1, Black No. 401 and other pigments, Yellow No. 4 Al lake, Yellow 203 No. Ba lake and other lake pigments; nylon powder, silk powder, urethane powder, Teflon (registered trademark) powder , Silicone powder, polymethylmethacrylate powder, cellulose powder, silicone elastomer spherical powder,
Polymers such as polyethylene powder; colored pigments such as yellow iron oxide, red iron oxide, black iron oxide, chromium oxide, carbon black, ultramarine blue and navy blue; white pigments such as zinc oxide, titanium oxide and cerium oxide; talc, mica, Extender pigments such as sericite, kaolin and tabular barium sulfate; pearl pigments such as titanium mica; metal salts such as barium sulfate, calcium carbonate, magnesium carbonate, aluminum silicate and magnesium silicate;
Inorganic powders such as silica and alumina; bentonite, smectite, boron nitride and the like can be mentioned. The shape of these powders is spherical, rod-shaped, needle-shaped, plate-shaped, irregularly-shaped, scaly, spindle-shaped or the like.

These powders are conventionally known surface treatments such as fluorine compound treatment, silicone treatment, silicone resin treatment, pendant treatment, silane coupling agent treatment, titanium coupling agent treatment, oil agent treatment, N-acylation. It may or may not be surface-treated in advance by lysine treatment, polyacrylic acid treatment, metal soap treatment, amino acid treatment, inorganic compound treatment, plasma treatment, mechanochemical treatment and the like.

Among these powders, silicone elastomer spherical powder, polyethylene powder, polypropylene powder, Teflon (registered trademark) powder, silicone rubber, urethane powder, polyalkylsilsesquioxane, nylon, silica beads, alumina beads, Spherical powders (including hollow resin powders) such as apatite and allylic acrylic beads are preferably blended because they have an excellent effect of retaining physiologically active ingredients and gradually releasing them.

Examples of the oil agent include volatile and non-volatile oil agents, solvents and resins usually used in skin cosmetics, and they may be liquids, pastes or solids at room temperature, but liquids excellent in handling are preferred. . Examples of the oil agent include higher alcohols such as cetyl alcohol, isostearyl alcohol, lauryl alcohol, hexadecyl alcohol, and octyldodecanol; fatty acids such as isostearic acid, undecylenic acid, and oleic acid;
Polyhydric alcohols and sugars such as glycerin, sorbitol, ethylene glycol, propylene glycol, polyethylene glycol; myristyl myristate, hexyl laurate, decyl oleate, isopropyl myristate, hexyldecyl dimethyloctanoate, glyceryl monostearate, diethyl phthalate , Esters such as ethylene glycol monostearate and octyl oxystearate; hydrocarbons such as liquid paraffin, petrolatum and squalane; waxes such as lanolin, reduced lanolin and carnauba wax; mink oil, cocoa butter, coconut oil, palm kernel oil, Fats and oils such as camellia oil, sesame oil, castor oil, and olive oil; and ethylene / α-olefin / cooligomers.

Examples of other types of oil agents include, for example, dimethylpolysiloxane, methylhydrogenpolysiloxane, methylphenylpolysiloxane, polyether-modified organopolysiloxane, fluoroalkyl / polyoxyalkylene co-modified organopolysiloxane. , Silicone compounds such as alkyl-modified organopolysiloxane, terminal-modified organopolysiloxane, fluorine-modified organopolysiloxane, amodimethicone, amino-modified organopolysiloxane, silicone gel, acrylic silicone, trimethylsiloxysilicic acid, silicone RTV rubber; perfluoropolyether , Fluorinated pitch, fluorocarbon, fluoroalcohol and other fluorine compounds.

Examples of the solvent include purified water, cyclic silicone, ethanol, light liquid isoparaffin, lower alcohol, ethers, LPG, fluorocarbon, N-methylpyrrolidone, fluoroalcohol, volatile linear silicone, and next-generation chlorofluorocarbon. Is mentioned.

As the surface active agent, for example, an anionic surface active agent, a cationic surface active agent, a nonionic surface active agent, and a betaine surface active agent can be used.

Examples of the tackifier and resin are sodium polyacrylate, cellulose ether, calcium alginate, carboxyvinyl polymer, ethylene / acrylic acid copolymer, vinylpyrrolidone polymer, vinyl alcohol / vinylpyrrolidone copolymer, nitrogen. Substituted acrylamide polymer, polyacrylamide, cationic polymer such as cationized guar gum, dimethyl acrylic ammonium polymer, acrylic acid methacrylic acid acrylic copolymer, POE / POP copolymer, polyvinyl alcohol, pullulan, agar, gelatin, tamarind seed Polysaccharides, xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum, gum arabic, crystalline cellulose, arabinogalactan, karaya gum, tragacanthga , Alginic acid, albumin, casein, curdlan, gellan gum, dextran, cellulose, polyethyleneimine, highly polymerized polyethylene glycol, cationized silicone polymer, synthetic latex, and the like.

It is also preferable that the skin cosmetic of the present invention has an ultraviolet protection effect. In this case, it is preferable to use an ultraviolet protective agent (also referred to as an ultraviolet absorbent) as shown below. The compounding amount of the ultraviolet protective agent is preferably 0.005 to 50% by weight, more preferably 0.01 to 40% by weight, based on the total amount of the skin cosmetic.

UV protector (including organic type and inorganic type. U
Examples of the inorganic type include fine particle titanium oxide, fine particle zinc oxide, and the like. Examples of organic UV protectors include 2-ethylhexyl paramethoxycinnamate, 2-hydroxy-4-methoxybenzophenone, and 2-hydroxy-4.
-Methoxybenzophenone-5-sulfuric acid, 2,2'-dihydroxy-4-methoxybenzophenone, p-methoxyhydrocinnamic acid diethanolamine salt, paraaminobenzoic acid (hereinafter abbreviated as PABA), ethyldihydroxypropyl PABA, glyceryl PABA, salicylic acid Homomenthyl, methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano-3,3-diphenylacrylate, octyldimethyl PABA, octyl methoxycinnamate, octyl salicylate, 2-phenyl-benzimidazole-5-sulfate, trisalicylic acid. Ethanolamine, 3- (4-methylbenzylidene) camphor, 2,
4-dihydroxybenzophenine, 2,2 ', 4,4'
-Tetrahydroxybenzophenone, 2,2'-dihydroxy-4,4'-dimethoxybenzophenone, 2-hydroxy-4-N-octoxybenzophenone, 4-isopropyldibenzoylmethane, butylmethoxydibenzoylmethane, 4-tert-butyl -4'-methoxydibenzoylmethane, 4- (3,4-dimethoxyphenylmethylene) -2,5-dioxo-1-imidazolidine propionate 2-ethylhexyl, polymer derivatives thereof, silane derivatives and the like. Further, these may be sealed in a polymer.

The skin cosmetics of the present invention include, for example, lotions, emulsions, skin milks, creams, ointments, lotions, calamine lotions, sunscreens, tanning agents, aftershave lotions, preshave lotions, makeup bases and packs. , Cleansing products, facial cleansers, acne-preventing cosmetics, basic cosmetics such as essences; foundations, white powder, eye shadows, eye liners,
Make-up cosmetics such as eyebrow, cheek, lipstick, nail color; shampoo, conditioner, conditioner,
Hair color, hair tonic, setting agent, hair styling agent, hair restorer, body powder, deodorant, depilatory agent, soap, body shampoo, hand soap, perfume, toothpaste, oral care product, bath agent and the like.

The dosage form of the skin cosmetic of the present invention is not particularly limited, and may be cream, emulsion, lotion,
Ointment, gel, spray, mousse, water-in-oil emulsion, oil-in-water emulsion, solid, sheet,
It can be applied to usual pharmaceuticals such as powders, quasi drugs, and cosmetics.

[0039]

EXAMPLES Next, the present invention will be described in more detail with reference to Examples of the present invention, Comparative Examples and use examples showing the effects of the present invention. These do not limit the scope of the invention. All "parts" are "parts by weight" unless otherwise specified.
"%" Indicates "% by weight" unless otherwise specified.

[Test Example 1] (Measurement of β-glucan content) β-glucan was analyzed by using a β-glucan measurement kit manufactured by Megazyme and McCle.
The ary method (enzymatic method) was used. First, when the measurement sample is a powder, it is sifted through a 500 μm (30 mesh) sieve, the water content is measured, 100 mg thereof is put into a 17 ml tube, and 200 μl of a 50% ethanol solution is added,
Dispersed. Then 4 ml of 20 mM phosphate buffer (pH
6.5) was added and mixed well, and then heated in a boiling water bath for 1 minute. Mix well and heat for another 2 minutes in a hot water bath. After cooling to 50 ° C. and leaving for 5 minutes, 200 μl (10 U) of lichenase enzyme solution (the vial attached to the kit was diluted with 20 ml of 20 mM phosphate buffer, the remaining amount was frozen and stored) was added to each tube, 1 hour, 50 ° C
It was made to react with. 200 mM acetate buffer (p
5 ml of H4.0) was added and mixed gently. 5 at room temperature
After allowing to stand for a minute, the supernatant was obtained by centrifugation. 100 μl for 3
Take one tube with 100 μl of 50 mM acetate buffer (pH 4.0) and the other two with 100 μl.
(0.2 U) β-glucosidase solution (a vial attached to the kit was diluted with 20 ml of 50 mM acetate buffer, and the remaining amount was stored frozen), and reacted at 50 ° C. for 10 minutes. 3 ml of glucose oxidase / bell oxidase solution was added and reacted at 50 ° C. for 20 minutes, and the absorbance (EA) at 510 nm of each sample was measured.
The β-glucan content was calculated by the following formula.

Βglucan (%, W / W) = (EA)
× (F / W) × 8.46 F = (100) / (absorbance of glucose 100 μg) W = calculated anhydrate weight (mg)

When the measurement sample was an extract (liquid) obtained by extracting β-glucan, the content was measured as an extract (solid or powder) as follows. That is, a β-glucan extract (solid) was obtained by adding twice the amount of ethanol to the β-glucan extract and mixing them well, then collecting the precipitate by centrifugation, thoroughly drying and pulverizing. The β-glucan extract was analyzed by the McCleary method (enzymatic method) using a β-glucan measuring kit manufactured by Megazyme after measuring the water content. 17 ml of 50 mg of each precipitated sample
It was taken in a tube and 200 μl of a 50% ethanol solution was added to disperse it. After that, the same measurement as above was performed.

Test Example 2 (Measurement of Molecular Weight) The molecular weight of the extract was measured as follows. That is, take 5 mg of the extract in a tube,
0.5 ml of distilled water was added and dissolved in boiling water.
A sample for HPLC was passed through a 0.22 μm filter. Sh is a HPLC gel filtration column for separation.
ode packed column KS-805 (manufactured by Showa Denko KK) using a flow rate of 0.6 ml / min. , Temperature 50 ℃,
An RI detector was used for detection, and water was used as the separation solvent. As a molecular weight marker, Shodex pullulan standard solution P-82
(Manufactured by Showa Denko KK).

When the extracted β-glucan was an extract (liquid), first, twice the amount of ethanol was added, the mixture was cooled to -20 ° C and left for 1 hour to obtain a precipitate. 5m of the obtained precipitate
Then, g was put in a tube, and thereafter, the same operation as in the case of the extract was performed to measure the molecular weight.

[Production Example of β-Glucan Raw Material and Extraction Accelerator] Sticky bare barley was ground with a grinding type polishing machine, and the barley was refined to a yield of 82%. The bran generated at this time was designated as bran-1. The barley that had been refined to a yield of 82% was further ground with a grinding type polishing machine to refine the barley to a yield of 55%. The bran generated at this time was designated as crushed product-1. 20 L of tap water was added to the container (50 L), and the temperature was adjusted to 15 ° C while stirring. 6 kg of bran-1 was added to this, and the mixture was extracted with stirring for 2 hours.
After solid-liquid separation with a continuous centrifuge, the supernatant was freeze-dried to obtain 450 g of an extraction accelerator.

[Example of β-Glucan Extraction] 30 L of tap water was added to a container (70 L), 150 g of the above-mentioned extraction accelerator was added with stirring, and after dissolution, 7.5 kg of the pulverized product-1 was added. After stirring and extracting at 50 ° C. for 2 hours, solid-liquid separation was performed with a continuous centrifuge and a supernatant was obtained. Boil the obtained supernatant,
After cooling, 15 L of slightly viscous β-glucan solution was obtained. To the obtained β-glucan solution, twice the amount of ethanol was added to collect the precipitate, which was dried to obtain 460 g of extracted β-glucan.
As a result of analysis according to Test Example 1, the purity of β-glucan is 91%.
Met. As a result of analysis according to Test Example 2, the extract was detected at a molecular weight of 100,000 to 10,000, and the maximum peak was at a molecular weight of 40,000. It was confirmed by the method of Test Example 1 that the maximum peak was β-glucan.

Using the extracted β-glucan obtained above,
Hereinafter, the skin cosmetic of the present invention was produced.

Example 1 A skin lotion base (invention product 1) was prepared according to the following formulation and production method.

[0049] [Compound] (parts by weight) Extracted β-glucan 10.0 Vaseline 2.0 Beeswax 0.5 Sorbitan sesquioleate 0.8 Polyoxyethylene oleyl ether 1.2 Ethanol 5.0 Carboxy vinyl polymer 20.0 (1% aqueous solution) Potassium hydroxide 0.1 Purified water 60.4

[Production Method] After adding ethanol to purified water, 7
The temperature is 0 ° C. and the aqueous phase is used. In addition, a lipophilic component other than the previously prepared aqueous carboxyvinyl polymer solution and potassium hydroxide is mixed and heated to 70 ° C. to form a lipophilic part. This lipophilic part is added to the aqueous phase part to carry out preliminary emulsification,
After the extracted β-glucan and carboxyvinyl polymer aqueous solution are added and uniformly mixed, potassium hydroxide is added to neutralize. Then, after uniformly emulsifying with a homomixer, 3
Cool to 0 ° C. to obtain a skin lotion base.

Example 2 A pack base (invention product 2) was prepared according to the following formulation and production method.

[0052]   [Compound] (parts by weight) Extracted β-glucan 0.03 Polyvinyl alcohol 15.0 Carboxymethyl cellulose sodium 5.0 Propylene glycol 3.0 Ethanol 10.0 Purified water 67.0

[Production Method] Propylene glycol is added to purified water and dissolved. Further, polyvinyl alcohol moistened with ethanol, sodium carboxymethyl cellulose and extracted β-glucan are added, and the mixture is heated to 70 ° C. and dissolved with stirring to obtain a pack base.

Comparative Example 1 Example 1 was repeated except that the extracted β-glucan was removed from the formulation of Example 1 and 5 parts by weight of squalane and 5 parts by weight of propylene glycol were added instead.
A skin lotion base (Comparative Product 1) was prepared in the same manner as in.

Comparative Example 2 A pack base (Comparative Product 2) was prepared in the same manner as in Example 2 except that the extracted β-glucan was omitted from the formulation of Example 2.

[Use Example 1] With respect to the products 1 and 2 of the present invention obtained in Examples 1 and 2 and the comparative products 1 and 2 obtained in Comparative Examples 1 and 2, imparting a feeling of wetness and softness to the skin The effect was measured as follows. That is, 16 women aged 18 to 57 years were used as evaluators, and one sample was used three times,
The effect of imparting a moist feel and softness to the skin was scored according to the following evaluation criteria.

<Evaluation criteria> 10 points; extremely effective 8 points; effective 6 points; somewhat effective 2 points; I feel effective 0 point; no effect

In this way, the average value (rounded down to the nearest whole number) used by one person three times is shown in Table 1 below.

[0059]

[Table 1]

As is apparent from the results shown in Table 1 above, the products 1 and 2 of the present invention containing the extracted β-glucan have an effect of imparting an appropriate moisturizing feeling and flexibility to the skin, and as a result, a moisturizing action on the skin. It can be seen that it has excellent anti-aging properties and skin-beautifying effects.

Example 3 (Production Example of Dentifrice) A dentifrice was produced by a conventional method according to the following formulation.

[0062] (Composition) (Parts by weight) Dicalcium phosphate 42 Glycerin 18 Carrageenan 0.9 Sodium lauryl sulfate 1.2 Saccharin sodium 0.09 Butyl paraoxybenzoate 0.005 Extracted β-glucan 0.05 Fragrance 1 Water level 100 in total

Example 4 (Manufacturing Example of Mouthwash) A mouthwash was manufactured by a conventional method according to the following formulation.

[0064] (Composition) (Parts by weight) Sodium lauryl sulfate 0.8 Glycerin 7 Sorbitol 5 Ethyl alcohol 15 Extracted β-glucan 0.1 1-menthol 0.05 Fragrance 0.04 Saccharin sodium 0.1 Water level 100 in total

Example 5 (Production Example of Soft Lotion (Weakly Acidic)) According to the following formulation
A soft lotion (weakly acidic) was produced by a conventional method.

[0066]   (Composition) (Parts by weight) Glycerin 5.0 Propylene glycol 4.0 Sodium hyaluronate 0.1 Extracted β-glucan 0.05 Polyoxyethylene sorbitan Monolauric acid ester (20E.O.) 1.5 Polyoxyethylene lauryl ether (20E.O.) 0.5 Ethanol 10.0 Perfume 0.1 Suitable amount of dye Preservative Suitable amount UV absorber suitable amount Purified water 78.75

Example 6 Production Example of Skin Cream A skin cream was produced by a conventional method according to the following formulation.

[0068]   (Composition) (Parts by weight) Beeswax 2.0 Stearyl alcohol 5.0 Stearic acid 8.0 Squalane 10.0 Self-emulsifying propylene glycol 3.0 Monostearate polyoxyethylene Cetyl ether (20.E.O.) 1.0 Fragrance 0.5 Preservative Suitable amount Antioxidant Appropriate amount Propylene glycol 7.8 Glycerin 4.0 Sodium hyaluronate 0.1 Extracted β-glucan 0.1 Triethanolamine 1.0 Purified water 57.5

Example 7 (Production Example of Lotion) A lotion was produced by a conventional method according to the following formulation.

[0070]   (Composition) (Parts by weight) Stearic acid 2.0 Cetanol 1.5 Vaseline 3.0 Lanolin alcohol 2.0 Liquid paraffin 10.0 Polyoxyethylene monooleate (10E.O.) 2.0 Fragrance 0.5 Antioxidant Appropriate amount Preservative Suitable amount Propylene Glycow 4.8 Glycerin 3.0 Sodium hyaluronate 0.1 Extracted β-glucan 0.1 Triethanolamine 1.0 Purified water 70.0

Example 8 Shampoo Production Example A shampoo was produced by a conventional method according to the following formulation.

[0072]   (Composition) (Parts by weight) Alkyl ether sodium sulfate (AES-Na) 16.0 Lauric acid diethanolamide 4.0 Propylene glycol 2.0 Extracted β-glucan 0.5 Preservative Suitable amount Dye suitable amount Fragrance suitable amount Purified water 77.5

Example 9 (Rinse Production Example) A rinse was produced by a conventional method according to the following formulation.

[0074]   (Composition) (Parts by weight) Stearyl dimethylbenzylammonium chloride 1.4 Stearyl alcohol 0.6 Glycerin monostearate 1.5 Salt 0.1 Extracted β-glucan 0.1 Purified water 96.3 100 in total

[0075]

EFFECTS OF THE INVENTION According to the present invention, it is possible to provide a skin cosmetic having excellent skin moisturizing action, anti-aging effect, beautiful skin effect and the like, and high safety.

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Yoshikazu Tokaibayashi             7-35 Higashiokyu, Arakawa-ku, Tokyo Asahiden             Chemical Industry Co., Ltd. F-term (reference) 4C083 AA082 AA111 AA112 AB032                       AB292 AB332 AC012 AC022                       AC072 AC102 AC122 AC132                       AC182 AC242 AC392 AC402                       AC442 AC482 AC542 AC642                       AC692 AC782 AC862 AD092                       AD112 AD211 AD212 AD272                       AD332 AD352 AD512 AD532                       BB51 CC04 CC05 CC07 CC38                       CC39 CC41 DD22 DD27 DD31                       EE12

Claims (6)

[Claims] 1. A skin cosmetic comprising a β-glucan extracted from a grass family plant. 2. A 1-2-β-D-glucopyranose bond, a 1-3-β-D-glucopyranose bond, 1-4-
The skin cosmetic according to claim 1, which contains β-glucan having two or more kinds of β-D-glucopyranose bonds and 1-6-β-D-glucopyranose bonds. 3. A β comprising a 1-3-β-D-glucopyranose bond and a 1-4-β-D-glucopyranose bond.
The skin cosmetic according to claim 2, which contains glucan. 4. The skin cosmetic according to claim 1, wherein the gramineous plant is barley or oats. 5. The β-glucan has a weight average molecular weight of 10
The skin cosmetic according to any one of claims 1 to 4, which is 10,000 or less. 6. The β-glucan content is 0.001 to 20% by weight with respect to the total amount of the skin cosmetics.
The β-glucan-containing skin cosmetic according to any one of 5 above.