JP2003189738A - Liquid-spawn packing bag, method and apparatus for inoculating liquid spawn using the bag - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method and an apparatus for relatively easily inoculating liquid spawn without significantly changing spawn inoculation facilities, to provide a readily portable packing bag for packing liquid spawn. <P>SOLUTION: The flexible liquid-spawn packing bag 2 in which liquid spawn B is subdivided and packed into small sizes from a spawn reserving tank 1 in which the liquid spawn B is stored while cultured under sterile conditions. The method for inoculating liquid spawn B comprises the following processes: a process of taking out spawn from the liquid-spawn packing bag 2 subdivided into small sizes under sterile conditions to supply the liquid spawn B to a liquid- spawn spraying mechanism 30 via a spawn supplying pipe 4 under pressure, and a run-off-amount setting process of performing switching operation to spray a predetermined amount of the liquid spawn to culture medium C in a cultivation container 18 from a spraying nozzle 36 provided on the liquid-spawn spraying mechanism under pressure. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an inoculum inoculation method and inoculation device for inoculating a culture medium filled in a mushroom cultivation container with a mushroom inoculum, and a filling bag for inoculating the inoculum.

[0002]

2. Description of the Related Art Conventionally, as a method for artificially cultivating mushrooms using a culture medium containing nutrients mainly composed of sawdust, rice bran, etc., the culture medium is filled in a cultivation container, sterilized, and the filled cultivation is carried out. After inoculating mushrooms such as enoki mushrooms and maitake mushrooms into the container, cover the mouth of the cultivation container with a lid and close it, and cultivate the bacteria under predetermined temperature and humidity conditions for industrial cultivation. The way to do is done.

By the way, as a seed culture generally used in the artificial cultivation of mushrooms using a conventional culture medium, the seed culture is in a seed culture bottle in a solid seed culture medium containing a nutrient source mainly containing sawdust or rice bran. Are bred in Japan. Since it usually takes more than 3 weeks to inoculate and culture this solid inoculum, it is necessary to have a space to store many inoculum bottles during inoculum culture. Need to secure a vast space. Therefore, for individual growers, it will be costly due to space issues, equipment issues, etc.
Mushrooms are cultivated by purchasing inoculum from inoculum centers.

At this time, when inoculating the solid inoculum in the inoculum bottle, the inoculum containing the inoculum is placed upside down and mounted on the body frame side of the inoculation device, and in this state Rotate, and insert it into the seed germ bottle while rotating the scraping shaft provided with a scraping blade from the mouth of the seed germ bottle, and gradually raise the scraping shaft while rotating the scraping spindle to scrape out the seed germ with the scraping blade, and the hopper. It is configured such that the seed bacteria that have been scraped out are filled into the cultivation container via the above.

Further, in the above-mentioned prior art, since the rotation amount of the scraping shaft is generally set depending on the kind of mushroom to be cultivated, the outer diameter of the mouth portion of the seed germ bottle set upside down is set. The amount of seed germs may be different due to the difference between the size and the outer diameter of the body,
In addition, since the inoculum itself in the inoculum bottle is solid, the size of the inoculum (sawdust) scraped out when the inoculum is scraped out may vary, and as a result, the filling amount of the inoculum may vary. In particular, when the amount of the seed bacteria filled is not more than a predetermined amount, it may be difficult for the hyphae of the seed bacteria to propagate in the cultivation container and it may take a long time for the culture.

[0006]

In consideration of this point, the development of an inoculation method using a liquid seed culture that has a shorter culturing period than that of a solid seed culture is underway, and a liquid form in a liquid seed culture culture tank is being developed. Various inoculum inoculation devices have been proposed for inoculating the inoculum by culturing and injecting the liquid inoculum in the liquid inoculum culture tank by spraying or spraying the liquid inoculum in the culture container in the cultivation container. For example, Japanese Patent Publication No. 47-41805
Japanese Unexamined Patent Publication No. 62-171618 proposes a structure in which a liquid inoculum is cultured in a liquid inoculum culture tank, and the liquid inoculum is taken out after the culture. Has been proposed as a method for artificially cultivating a seed culture in a liquid state, and inoculating the seed culture into a mushroom cultivating container at a stage when a rapid growth period in which the seed culture activity is increased is reached. In this case, the inoculated liquid inoculum is directly inoculated from the liquid inoculum culture tank through the inoculum extraction nozzle into the cultivation container in order to prevent invasion of miscellaneous bacteria during inoculation.

However, the above-mentioned liquid seed inoculation method or inoculation device is not disclosed in the above-mentioned Japanese Patent Laid-Open No. 62-62.
As disclosed in Japanese Patent Publication No. 171618, liquid seeds are stored in a liquid seed culture tank through several steps, and the device itself is large, so For individual growers, in addition to the inoculation process equipment, since expensive seed culture tanks, seed clean culture equipment such as indoor clean equipment and indoor air conditioning equipment are required, it is possible to introduce even liquid seed culture culture equipment. The reality is that you cannot do it. For this reason, it is assumed that the liquid seed culture culture tank that stores the liquid seed culture is transported to a small-scale company or the home of an individual grower, and the liquid seed culture in the liquid seed culture culture tank is inoculated.
Since the culture tank has a size of about 300 liters to 500 liters, it is difficult to carry it, and it is not suitable for micro enterprises and individual growers with a relatively small production quantity.

[0008] Apart from the above-mentioned problems, generally, in the surface part of the culture medium filled in the inside of a culture bottle or a culture bag, which is a culture container, the central part is directed from the top surface to the bottom of the culture medium. Has a hole for inoculation. This inoculation hole is provided to improve the surrounding of the bacteria in the culture medium, and improves the air permeability of the culture medium itself or when the culture medium is inoculated into the cultivation container (cultivation bottle, cultivation bag). The purpose is to make it easier for the inoculum to reach the bottom of the seeds.However, in the method or inoculation device for inoculating the liquid inoculum described above, the inoculum should not reach the floor surface and the inoculum of the culture medium. Therefore, there is still a problem that the surrounding of the bacteria cannot be performed well.

Therefore, the present invention solves the above-mentioned problems and is relatively simple without changing the seed inoculation equipment, and inoculates a liquid inoculum with a seed inoculation method, an inoculation device, and easily carried. Another object of the present invention is to provide a filling bag filled with a liquid inoculum capable of Another object of the present invention is to provide a liquid inoculum inoculating device capable of performing stable inoculum filling while maintaining a substantially constant inoculum supply amount.

[0010]

According to a first aspect of the present invention, there is provided a flexible liquid inoculum filling method in which the liquid inoculum is subdivided into an aseptic state from an inoculum storage tank in which the liquid inoculum is cultured and stored. It's a bag.

According to a second aspect, in the first aspect, the liquid seed-filled bag is provided with a supply port for allowing the liquid seed-filled bag in the liquid seed-filled bag to be discharged to the outside. Is.

According to a third aspect of the present invention, there is provided a liquid seed germ-filled bag according to any one of the first and second aspects, wherein the liquid seed germ-filled bag is an inner bag, and the outside thereof is covered with an outer box made of a hard material.

According to a fourth aspect of the present invention, there is provided a pouring step of pressurizing and supplying the liquid inoculum from the bag in which the liquid inoculum is subdivided into a sterile state through the inoculum supply pipe and sending out to the liquid inoculum injection mechanism. A liquid seed culture inoculation method comprising a flow rate setting step in which a switching operation is performed to pressurize and spray a predetermined amount of liquid seed culture from a spray nozzle provided in the liquid seed culture spraying mechanism to a culture medium of a cultivation container.

According to a fifth aspect of the present invention, in the method for inoculating a liquid inoculum according to the fourth aspect, when inoculating with a spray nozzle provided in the liquid inoculum injection mechanism, the liquid is formed mainly on the floor surface position of the culture medium in the cultivation container. It is a method of inoculating a liquid inoculum comprising a step of inoculating the inoculum and a step of inoculating the liquid inoculum mainly based on the position of the inoculation hole formed in the floor surface portion of the culture medium.

According to a sixth aspect of the present invention, in an inoculating device for inoculating a culture medium in a cultivation container with a seed culture, the liquid seed culture is stored in a seed storage tank for culturing and storing the liquid seed in a sterilized state. A liquid seed culture filling bag having, a seed culture feed line for supplying the liquid seed culture from the liquid seed culture filling bag, and the liquid seed culture is pressurized and sent out on the way of the liquid seed culture filling bag or the transport route of the seed culture supply pipe. Liquid inoculum pressure supply means for, a liquid inoculum injection mechanism for spraying and inoculating the liquid inoculum pressurized and supplied from the liquid inoculum pressure supply means to the culture medium of the cultivation container, and this liquid inoculum injection mechanism A liquid inoculum provided with a spray opening / closing valve which is provided and supplies and shuts off the liquid inoculum that has been pressure-fed from the liquid inoculum filling bag through the inoculum supply conduit. A vaccination apparatus.

According to a seventh aspect of the present invention, there is provided a liquid inoculum inoculation device according to the sixth aspect, comprising valve opening / closing drive control means for switching the spray on / off valve to set a flow rate or an inoculation time for inoculating the liquid inoculum. is there.

According to an eighth aspect, in the sixth or seventh aspect, the liquid inoculum injection mechanism is provided with an elevating mechanism capable of moving toward and away from the cultivation container,
At a predetermined operating position by this elevating mechanism, the liquid inoculum is sprayed and inoculated mainly with the floor surface position of the culture medium in the cultivation container by the liquid inoculum injection mechanism, and the floor surface portion of the culture medium is perforated. The liquid inoculum inoculating device is configured to spray and inoculate the liquid inoculum mainly on the position of the inoculated holes.

Further, in claim 9, claim 6 to claim 8
In the above, the liquid inoculum inoculation device is formed by a packaging bag made of synthetic resin or the like as the cultivation container.

According to a tenth aspect of the present invention, in the sixth to eighth aspects, the cultivation container is formed by a cultivation bottle with a lid made of synthetic resin or the like, and a plurality of the cultivation bottles are vertically and horizontally aligned and stored. A transport mechanism that supplies the container from the loading side and transports the container to the unloading side while intermittently transporting the cultivation bottle, and the lid of the cultivation bottle inside the container that has been transported to the seed inoculation position by this transportation mechanism in order. A lid opening / closing mechanism that opens and closes a predetermined number, and is provided according to the number of cultivation bottles that are opened and closed by the lid opening and closing mechanism, and is directed toward the culture medium in the cultivation bottle whose lid is opened by the lid opening and closing mechanism. And a liquid inoculum injection mechanism for spraying and inoculating the liquid inoculum.

[0020] According to an eleventh aspect of the present invention, in the sixth to eighth aspects, the cultivation container is formed of a cultivation bottle with a lid made of synthetic resin or the like, and the cultivation bottles are arranged in a plurality of rows and columns and stored. A transport mechanism that supplies the container from the loading side and transports the container to the unloading side while intermittently transporting the cultivation bottle, and a lid of the cultivation bottle in the container that is intermittently transported by this transportation mechanism in a horizontal row unit. A lid opening / closing mechanism that is opened and closed, and a liquid inoculum injection mechanism that supplies a predetermined amount of liquid inoculum to each of the cultivation bottles in a horizontal row whose lid is opened by the lid opening / closing mechanism, respectively. This is a liquid inoculum inoculation device.

[0021]

BEST MODE FOR CARRYING OUT THE INVENTION According to the first aspect of the present invention, since the bag is a liquid seed-filled bag having flexibility, which is obtained by aseptically filling the liquid seeds in a sterile storage tank for storing the liquid seeds in a sterile state. Since it is not necessary to transport a seed storage tank for cultivating and storing heavy liquid seeds, the compact and easy-to-carry liquid seed filling bag is filled with the liquid seeds for good operability and in a sterilized state. Since the liquid inoculum is enclosed and packed, the inoculation work, which is the next step, can be performed easily.

Further, according to the invention of claim 2, the liquid seed bacteria-filled bag is provided with a supply port capable of pouring out the liquid seed bacteria in the liquid seed bacteria-filled bag to the outside. It is possible to supply the liquid inoculum to the outside.

In the invention of claim 3, the liquid seed germ-filled bag is used as an inner bag, and the outer side of the bag is covered with an outer box made of a hard material. It can be protected by covering and can be carried easily.

Further, in the invention of claim 4, there is provided a pouring step of pressurizing and supplying the liquid inoculum from the bag in which the liquid inoculum is subdivided into a sterile state through the inoculum supply line to the liquid inoculum injection mechanism. The method for inoculating a liquid inoculum comprising a flow rate setting step in which a predetermined amount of the liquid inoculum is pressurized and sprayed from a spray nozzle provided in the liquid inoculum injection mechanism to a culture medium of a cultivation container to perform a switching operation. As it is subdivided by the seed-filled bag, the operability is good,
Supplying liquid inoculum to the liquid inoculum injection mechanism side while pressurizing,
In the flow rate setting step, it is possible to spray while injecting a predetermined amount of liquid inoculum from the injection nozzle provided in the liquid inoculum injection mechanism, thereby inoculating and inoculating the culture medium of the cultivation container with a predetermined amount of liquid inoculum. You can

Further, in the invention of claim 5, the step of inoculating the liquid inoculum mainly on the floor surface position of the culture medium in the cultivation container at the time of inoculation by the injection nozzle provided in the liquid inoculum injection mechanism, Since it is a method of inoculating liquid inoculum mainly consisting of inoculating the liquid inoculum mainly on the position of the inoculation hole drilled in the floor part, the floor part which is the surface part of the culture medium in the cultivation container and its floor It becomes possible to inoculate the liquid inoculum evenly on each surface part of the inoculation hole that is formed by denting a part of the surface part, which can favorably promote the growth of hyphae on the culture medium by the inoculum. It will be possible.

Further, in the invention of claim 6, in the inoculation device for inoculating the culture medium in the cultivation container with the inoculum, the liquid inoculum is subdivided and filled in a sterilized state from an inoculum storage tank in which the liquid inoculum is cultured and stored. A liquid inoculum filling bag having flexibility, an inoculum supply line for supplying a liquid inoculum from this liquid inoculum fill bag, and a liquid inoculum is pressurized in the transport route of the liquid inoculum fill bag or the inoculum supply line. Liquid inoculum pressurizing and supplying means for delivering the liquid inoculum, a liquid inoculum injection mechanism for spraying and inoculating the liquid inoculum pressurized and supplied from the liquid inoculum pressurizing and supplying means to the culture medium of the cultivation container, and this liquid inoculum injection Provided in the mechanism,
Since the liquid inoculum inoculation device comprises a spray on-off valve for supplying and shutting off the liquid inoculum that has been pressure-fed from the liquid inoculum filling bag through the inoculum supply line, it is compact and easy to carry. By filling the bag with liquid seeds, the operability can be improved and the bag can be connected to the seed supply line provided in the inoculation device, and the liquid seeds can be directly pressurized by the liquid seed pressurizing supply means. It is supplied to the inoculum injection mechanism side and the injection on / off valve is operated, so that the amount of inoculum supplied from the liquid inoculum filling bag can be sent out at a substantially constant amount, whereby a predetermined amount of inoculum is sprayed onto the culture medium of the cultivation container. Is what you can do.

According to a seventh aspect of the invention, there is provided a liquid inoculum inoculation device comprising valve opening / closing drive control means for switching the spray on / off valve to set a flow rate or inoculation time for inoculating the liquid inoculum. Adjusting and setting the inoculum filling amount of the inoculum by the valve opening / closing drive control means according to the injection form of the inoculum injection mechanism and the diameter of the injection port, or according to the capacity and form of the culture medium in the cultivation container or the medium of the culture medium It is possible to maintain a substantially constant amount while setting the filling amount of the inoculum without any variation, and a stable inoculation process work can be performed.

Further, in the invention of claim 8, the liquid inoculum injection mechanism is provided with an elevating mechanism capable of moving toward and away from the cultivation container, and at a predetermined operating position by the elevating mechanism, the liquid state While inoculating by spraying a liquid inoculum mainly on the floor surface position of the culture medium in the cultivation container by the inoculum injection mechanism, the liquid inoculum mainly on the inoculation hole position perforated in the floor surface portion of the culture medium Since it is a liquid inoculum inoculating device configured to be sprayed and inoculated, at a predetermined operating position by the elevating device, the floor surface portion which is the surface portion of the culture medium in the cultivation container and the floor surface portion thereof. The liquid inoculum can be uniformly inoculated by the liquid inoculum injection mechanism into the respective surface portions of the inoculation hole formed by denting a part of the inoculum.

[0029] According to the invention of claim 9, in any one of claims 6 to 8, the culture container is formed of a packaging bag made of synthetic resin or the like, so that the culture substrate is provided from the bag mouth side of the packaging bag. A predetermined amount of liquid inoculum can be inoculated toward the target.

[0030] According to the invention of claim 10, in claim 6 to claim 8, the cultivation container is formed by a cultivation bottle with a lid made of synthetic resin or the like, and a plurality of the cultivation bottles are vertically and horizontally aligned. A transport mechanism that supplies the stored container from the loading side and transports the container to the unloading side while intermittently transporting the cultivation bottle, and a lid for the cultivation bottle in the container that has been transported to the seed inoculation position by this transportation mechanism. And a lid opening / closing mechanism that sequentially opens and closes a predetermined number of pieces, and a culture medium in the cultivation bottle that is provided according to the number of cultivation bottles that are opened and closed by the lid opening and closing mechanism, and the lid is opened by the lid opening and closing mechanism. A liquid inoculum inoculation device comprising: the liquid inoculum spraying mechanism for spraying and inoculating liquid inoculum toward a plant, wherein the lid of the cultivation container is covered and opened / closed in a state of being stored in the container. In addition to opening and closing a predetermined number of cells, the liquid inoculum injection mechanism inoculates the liquid inoculum when opening the lid, so the inoculation work efficiency of the liquid inoculum can be increased and the opening time of the lid of the cultivation container is shortened as much as possible. Then, invasion of various bacteria can be suppressed. After the inoculation process is completed, the cultivation container housed in the container is transferred to the unloading side by the transfer mechanism. Further, at a predetermined operating position by the lifting device, each surface portion of a floor surface portion which is a surface portion of the culture medium in the cultivation container and an inoculation hole formed by being recessed in a part of the floor surface portion. In addition, it is possible to uniformly inoculate the liquid inoculum with the liquid inoculum injection mechanism. This makes it possible to favorably promote the growth of hyphae inside the culture medium by the inoculum.

According to the invention of claim 11, in claim 6 to claim 8, the cultivation container is formed by a cultivation bottle with a lid made of synthetic resin or the like, and a plurality of cultivation bottles are aligned vertically and horizontally. A transport mechanism that supplies the stored container from the loading side and transports the container to the unloading side while intermittently transferring the cultivation bottle, and a lid of the cultivation bottle in the container that is intermittently transferred by the transportation mechanism in a horizontal row. A lid opening and closing mechanism that operates to open and close in units, and the liquid inoculum injection mechanism that supplies a predetermined amount of liquid inoculum to each of the cultivation bottles in a horizontal single row unit whose lid is opened by the lid opening and closing mechanism. A liquid seed culture inoculation device provided, which is intermittently transferred to the seed culture inoculation position by the transport mechanism, and the lid of the cultivation container in the container opens and closes in horizontal row units at the seed culture inoculation position. Opened by the closing mechanism, a predetermined amount of liquid inoculum is supplied to the cultivation container in the horizontal row unit whose lid is opened by the lid opening and closing mechanism, respectively, is inoculated by being supplied to the cultivation container through the liquid inoculum injection mechanism, After the inoculation, the lid is closed by the lid opening / closing mechanism, and the cultivation mechanism in the container is intermittently transferred to the unloading side in horizontal row units by the transport mechanism based on this repetition. At the time of this inoculation, the lid of the cultivation container in the container is opened in horizontal row units by the lid opening / closing mechanism, and at the time of opening, the liquid inoculum is inoculated by the liquid inoculum injection mechanism, so the inoculation work efficiency of the liquid inoculum can be improved. In addition, the opening time of the lid of the cultivation container can be shortened as much as possible, and invasion of various bacteria can be suppressed. Further, at a predetermined operating position by the lifting device, each surface portion of a floor surface portion which is a surface portion of the culture medium in the cultivation container and an inoculation hole formed by being recessed in a part of the floor surface portion. In addition, since it is possible to uniformly inoculate the liquid inoculum with the liquid inoculum injection mechanism, it is possible to favorably promote the growth of hyphae inside the culture medium by the inoculum.

[0032]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS A first embodiment of the inoculation device for liquid seed bacteria according to the present invention will be described below with reference to FIGS. 1 to 29.
FIG. 1 shows a schematic diagram of the whole structure of an inoculum storage tank, a liquid inoculum filling bag, and an inoculation device for liquid inoculum.
It is cultured and stored in a metal seed culture storage tank 1 having a size of 1 to 500 liters. From this seed culture storage tank 1, the liquid seed culture B is stored in a flexible liquid seed culture filling bag 2 while maintaining a sterile condition. Each is divided into small parts, filled, sealed and stored. The subdivided aseptic liquid seed culture-filled bag 2 is transported to each grower, and the liquid seed germ B is supplied to the grower. Further, at the time of inoculation by each grower, the liquid seed germ filling bag 2 and the inoculation device 3 are connected via the seed germ supply pipe 4 made of a flexible tube, and the liquid seed germ B in the liquid seed germ filling bag 2 is connected. Is pressurized and supplied to the inoculation device 3 side.

The liquid seed germ-filled bag 2 of the first embodiment is provided with a supply port 5 as shown in FIG. 2, and a suction pipe 6 is inserted into the liquid seed germ-filled bag 2 from the supply port 5. Are arranged. Further, the liquid seed germ-filled bag 2 itself is provided so as to be housed in the pressure vessel 7 which is a hard outer box. To the pressure vessel 7 side, in order to supply the liquid inoculum B from the liquid inoculum filling bag 2 to the inoculation device 3 side via the inoculum supply line 4, the liquid inoculum added by a compressor is added. The liquid inoculum-filled bag 2 is connected by a pressure means 8 via a pressure air hose 9 so that it can be pressurized by air pressure from the outside. At that time, a pressure adjuster 10 for adjusting the pressure for pressurizing the liquid seed germ-filled bag 2
Is interposed between the liquid inoculum pressurizing means 8 and the pressurizing container 7 so that the air pressure can be set.

As the inoculation device 3, the inoculation device 3 is used.
A transport mechanism 12 including a conveyor is provided over substantially the entire length of the main body frame 11, and the transport mechanism 12 can be started and stopped by operating a switch on the operation panel 13. A plurality of sprocket wheels 14 are rotatably supported at both ends of the body frame 11, and an endless chain 15 is laid in parallel between the sprocket wheels 14 provided at both ends of the body frame 11 to connect to the drive source side. The rotational force generated by the motor 16 is transmitted to the sprocket wheel 14.

Then, a cultivation container 18 made of a cultivation bottle made of synthetic resin, which is filled with a culture medium C such as sawdust and rice bran and sealed with a lid 17, is a container 19 made of synthetic resin containing, for example, four columns and four rows. Is placed on the chain 15 of the transport mechanism 12 composed of a conveyor at the transfer start side, and the transport mechanism 12 is started so that the container 19 can be transported toward the transfer end side by traveling of the chain 15.

By means of the transport mechanism 12 provided in the main body frame 11, the container 19 in which a plurality of cultivation containers 18 are vertically and horizontally arranged and stored is transferred from the loading side to the area for inoculating the inoculum, and the inoculation area for the inoculum is formed. At the container 1
9 and the cultivation container 18 are intermittently transferred. In this case, as a means for stopping the container 19 sent through the transport mechanism 12 when the cultivation container 18 reaches the proper seed inoculation area, the stopper mechanism 20 having the stopper pin 20A in this embodiment is used. A cultivation container positioning and holding mechanism 21 that is provided and that can position and hold the cultivation container 18 when opening and closing the lid 17 while positioning the cultivation container 18 in the container 19 is provided.

In this case, the interval between the stopper pins 20A provided in the stopper mechanism 20 of the container 19 for stopping the container 19 at a predetermined position is set such that the cultivation containers 18 stored in the container 19 are arranged in a vertical row (4 pieces). ) Are arranged in four rows according to the intervals of the cultivation containers 18 arranged in
Each stopper pin 20A of the stopper mechanism 20 is provided so as to be movable up and down via an air cylinder 20B which is a driving means, and each stopper pin 20A is independently and constantly urged upward from the transport surface of the transport mechanism 12. It is provided via a spring 20C so as to be biased.

Further, when the transport mechanism 12 transports the cultivation containers 18 housed in the container 19 to the inoculation area position, the cultivation containers 18 are arranged in a horizontal row.
As the cultivation container positioning and holding mechanism 21 capable of positioning and holding the shoulder part of the cultivation container, the shoulder part of the cultivation container 18 positioned on the outer side and arranged in the horizontal single row unit is adjacent to each other in the horizontal single row unit. Shoulder holding arm members 22 that respectively hold the shoulders of the cultivation container 18 are provided. In this embodiment, the cultivation containers 18 are arranged in horizontal rows.
Since there are four, the shoulder holding arm members 22 for holding the shoulders of the respective cultivation containers 18 are arranged, and each shoulder holding arm member 22 is a holding arm mounting shaft. 23 is inserted into and fixed to the holding arm mounting shaft 23, and both ends of the holding arm mounting shaft 23 are mounted and fixed to the elongated holes provided in the L-shaped arm 24 through fixing bolts or screws so that the position can be adjusted. The tip of the arm 24 is
Spindle 2 provided on the body frame 11 side of the inoculum inoculation device
It is rotatably supported with 5 as a fulcrum. A drive cylinder 27, which is a drive means, is attached and fixed to the base end of the L-shaped arm 24 via a connecting rod 26, and each drive cylinder 27 is attached to a cylinder support shaft 28 spanning the main body frame 11. It is fixed.

Therefore, the L-shaped arm 24 is pushed by pushing the connecting rod 26 by the operation of the drive cylinder 27.
Rotates about the support shaft 25, and the pressing arm attachment shaft 23 fixedly attached to the intermediate portion of the L-shaped arm 24 and the shoulder pressing arm member 22 attached to the attachment shaft 23 in a non-rotating state. Rotate with the position of the support shaft 25 as the base point,
The cultivation container 18 is configured to be held at a predetermined position by pressing the shoulder portion of the cultivation container 18 in which the tip of the shoulder pressing arm member 22 is aligned in a horizontal row. Note that urethane rubber or the like is attached to the back surface of the tip of the shoulder pressing arm member 22, and
In order to prevent a large load from being directly applied to the cultivation container 18, a flexible resin tube is fitted.

Further, the container 1 is carried by the carrying mechanism 12.
When the cultivation container 18 housed in the container 9 is transported to the seed inoculation area position, the cultivation container 18 is arranged in horizontal row units.
Is positioned and held by the cultivation container positioning and holding mechanism 21, and a lid opening / closing mechanism 29 for opening and closing the lids 17 of the cultivation containers 18 arranged in horizontal row units is arranged above the seed culture inoculation area. ing.

In addition, when the lids 17 in horizontal rows arranged at the front row position of the cultivation vessels 18 are opened by the operation of the lid opening / closing mechanism 29, the cultivation containers 18 in horizontal rows are opened and aligned. Liquid inoculum B is sprayed through the liquid inoculum injection mechanism 30 arranged in one row (4) in accordance with the number (4) of the cultivation containers 18 in a horizontal row unit to the culture medium C. The opened culture container 18 is filled with the liquid inoculum B and inoculated. In this embodiment, for example, as disclosed in Japanese Patent Application No. 2000-323298 proposed by the applicant of the present application, as the liquid seed germ injection mechanism 30 for supplying the liquid seed germ B into the cultivation container 18, The liquid inoculum jetting mechanisms 30 are arranged in one row on the upper side through which the cultivation containers 18 pass, and the number of liquid inoculum injection units 30 is the same as the number of four cultivation containers 18 as one horizontal row unit. They are installed at equal intervals, but depending on the outer diameter of the cultivation container 18 and the size of the container 19, the number of horizontal row units may be 5, or may be set to 6. When inoculating the cultivation containers 18 arranged in the inside 19, the lid opening / closing mechanism so that two rows can be inoculated simultaneously as disclosed in Japanese Patent Application No. 2000-389972 proposed by the applicant of the present application. 29 and liquid It is also possible to configure so that bacteria injection mechanism 30 can inoculated once a liquid inoculum B arranged in two rows, respectively, in which case, may be appropriately set according to specifications.

In addition, the liquid inoculum B is placed in the cultivation container 18 in horizontal row units opened by the liquid inoculum injection mechanism 30.
After filling and inoculating, the lid 17 is attached to the cultivation container 18 by the return operation of the lid opening / closing mechanism 29. After the closure of the lid 17 is completed, the operation of the cultivation container positioning and holding mechanism 21 that holds the cultivation container 18 is resumed, so that the liquid inoculum B is inoculated into the lateral one-row cultivation container 18 located in the front row. It Next, the container 19 is transferred by the transport mechanism 12.
Is intermittently transferred and four cultivation containers 18 arranged in the second row of the cultivation container 18 housed in the container 19 are held by the operation of the cultivation container positioning mechanism 21, and the second The lid opening / closing mechanism 29 opens and closes the lid 17 of the row cultivation container 18 in units of one horizontal row,
Similarly, after injecting the liquid inoculum B by spraying the liquid inoculum B by the four liquid inoculum injection mechanisms 30 arranged in a horizontal row unit, the lid 17 is moved to the cultivation container 18 by the return operation of the lid opening / closing mechanism 29. Adhesion, followed by cultivation container positioning mechanism 2
The return operation of 1 completes the inoculation process of the second row. Then, in the third and fourth rows of the cultivation containers 18 arranged in the container 19 as well, after the inoculation is completed to the culture medium C of all the cultivation containers 18 in the container 19, the transport mechanism 12 Thus, the container 19 is transferred to the unloading side.

In the first embodiment, as described above, the liquid seed germ-filled bag 2 in which the liquid seed germ B is subdivided from the seed germ storage tank 1 in an aseptic state is supplied to the supply port 5 of the filling bag 2. It is configured to be connected to the liquid inoculum injection mechanism 30 side of the inoculation device 3 via the inoculum supply line 4 made of the tube.

At this time, the liquid seed germ-filled bag 2 itself is accommodated in the pressure vessel 7 which is a hard outer box as described above, and the liquid seed germ B is fed from the liquid seed germ filled bag 2 into the seed feed line. In order to pressurize and supply to the liquid inoculum injection mechanism 30 via 4, the liquid inoculum pressurizing means 8 composed of a compressor or the like applies air pressure from the outside of the liquid inoculum filling bag 2 via the pressurized air hose 9. I am trying.

The liquid seed culture B in the liquid seed culture filling bag 2 pressurized and supplied by the liquid seed culture pressurizing means 8 is sprayed onto the culture medium C in the cultivation container 18 by the liquid seed culture spraying mechanism 30 to be inoculated. The liquid inoculum B, which has been pressure-fed from the liquid inoculum filling bag 2 via the inoculum supply line 4, is configured to be supplied and shut off by the operation of the injection opening / closing valve 31 provided in the liquid inoculum injection mechanism 30. ing.

In this embodiment, the liquid inoculum injection mechanism 3 is used.
In order to reciprocally operate the injection opening / closing valve 31 for supplying and shutting off the liquid seed bacteria B provided at 0, the injection opening / closing valve 31
A gas pressurizing means 32 including a compressor for supplying air to the air is provided, and flexibility is provided via a filter 33 between the gas pressurizing means 32 and the liquid seed germ injection mechanism 30 provided with the injection on-off valve 31. A gas supply pipeline 34 made of a tube is connected, and an electromagnetic valve 35 for supplying and shutting off air as pressurized gas is interposed between the filter 33 and the liquid seed germ injection mechanism 30.

The filter 33 is provided for purifying the air sent from the gas pressurizing means 32, and is constructed so that various bacteria do not enter the inside of the liquid seed germ injection mechanism 30.

The liquid inoculum injection mechanism 30 in the first embodiment is located on the upper side through which the cultivation containers 18 pass, and is arranged in the same number as the four cultivation containers 18 which are the number of the horizontal rows. Liquid inoculum injection cylinder main body 37 in which each liquid inoculum injection mechanism 30 is provided with an injection nozzle 36 for spraying and inoculating the culture medium B in the cultivation container 18 on the tip side.
Is installed. This liquid seed germ injection cylinder body 37
As shown in FIG. 17 to FIG. 19, the inoculum supply port 37A connected to the inoculum supply line 4 side and the hollow form into which the liquid inoculum B can continuously flow from the inoculum supply port 37A. A fluid passage 37B is formed, and the injection nozzle 36 is attached and fixed to the tip end side of the hollow fluid passage 37B, and the injection nozzle 36 and the inoculum supply port 37A.
The injection opening / closing valve 31 for supplying and shutting off the liquid inoculum B that is pressure-fed is disposed in the hollow fluid passage 37B between and.

A valve element 31 provided on the injection opening / closing valve 31
A valve seat 37C, which is opened or closed according to the movement of the portion A, is formed in the middle of the hollow fluid passage 37B, and the liquid inoculum injection cylinder body 37 is formed.
A gas supply port 37D connected to the end of the gas supply pipeline 34 is provided on the rear end side. In addition, the injection nozzle 36
The injection opening / closing valve 31 with respect to the valve seat 37C provided in the middle of the hollow fluid passage 37B between the inoculum supply port 37A
The valve body 31A provided in the injection opening / closing valve 31 is operated to be opened or closed according to the reciprocating movement of (1), thereby supplying or shutting off the liquid inoculum B sent under pressure.

Further, the injection opening / closing valve 31 has a hollow fluid passage 37B provided in the liquid seed germ injection cylinder body 37.
The valve body 311A for opening and closing the valve body is provided, and the end portion of the piston shaft 31B continuously extending rearward from the valve body 31A is disposed so as to face the gas supply port 37D of the liquid inoculum injection cylinder body 37. . Further, a piston spring 31C is interposed between the large diameter portion provided on the piston shaft 31B and the liquid seed germ injection cylinder main body 37, and the valve body 31A is provided in the liquid seed germ injection cylinder main body 37 by the piston spring 31C. The fluid passage 37B is arranged so as to be constantly elastically urged toward the valve seat 37C.

Further, in order to keep the space between the hollow fluid passage 37B side provided in the liquid seed injection cylinder main body 37 and the gas supply port 37D provided at the rear end side of the liquid seed injection cylinder main body 37 watertight, A diaphragm 31D made of a flexible material such as synthetic rubber, which serves as an airtight member, is disposed between the shaft 31B and the liquid seed germ injection cylinder body 37.

Further, the injection nozzle 36 fixedly attached to the tip end of the hollow fluid passage 37B of the liquid inoculum injection cylinder main body 37 is set so as to have a predetermined injection pattern when the liquid inoculum B is injected into the inside thereof. Nozzle tip 3
6A is provided, and the liquid inoculum B pressurized from the ejection port 36B of the ejection nozzle 36 is configured to be ejected toward the culture medium C of the cultivation container 18.

The liquid inoculum injection cylinder main body 37 provided with the four injection nozzles 36 provided in the above-described liquid inoculum injection mechanism 30 is a plate-shaped nozzle that is detachably attached via nozzle fixing members 38 by fixing screws or the like. The nozzle mounting member 39 is fixedly held by the mounting member 39, and is detachably mounted and fixed to the support plate 40 on the main body frame 11 side of the inoculation device. In this case, the notch groove 3 is provided on both ends of the nozzle mounting member 39.
9A is provided, and the cutout groove 39A of the nozzle mounting member 39 is aligned with the support plate 40, and the fixing screw R is used to turn and fix the support plate 40.
Therefore, by loosening the fixing screw R, the nozzle mounting member 39 is removed from the support plate 40 by the cutout groove 39A.
It is provided so as to be removed along with. (Fig. 1
0, see Fig. 11, etc.)

The support plate 40 is provided so as to be vertically reciprocable by the operation of the air cylinder 41. The air cylinder 41 is fixed to the cylinder support plate 42 and is attached to the lower end side of the cylinder support plate 42. A fixed piece 42A is arranged so as to be horizontally reciprocable along a guide rod 43 provided on the main body frame 11 side.

Further, in the inoculum inoculation area, as the respective lid opening / closing mechanisms 29 for opening / closing the lids 17 of the cultivation containers 18 in horizontal row units, Japanese Patent Application Laid-Open No. 5-103542 previously proposed by the applicant of the present application is disclosed. As shown, a lid 1 for each cultivation container 18 positioned in a horizontal row.
7 is provided with a fixed sandwiching member 44 and a movable sandwiching member 45 composed of a sandwiching shaft that makes it possible to sandwich 7 from the upper surface side and the lower surface side, and the movable sandwiching member 44 can be driven forward and backward. Air cylinders 46 are provided between the movable pinching member 45 and the fixed pinching member 44 as pinching member opening / closing means for pinching and releasing the lids 17 in horizontal units. Each cylinder 46 is mounted and fixed on a cylinder holding plate 47 provided along the width direction of the main body frame 11 of the inoculation device main body, and the cylinder holding plate 4
A transfer moving means 48 composed of an inverted T-shaped link arm member is attached and fixed to an end portion of the cylinder holding plate 4.
The fixed pinching member 44, the movable pinching member 45 and the air cylinder 46 mounted on 7 are the link arm members 4
8 (transmission / moving means 48) so as to be movable between the lid closing position and the lid opening position of the cultivation container 18, and in this case, the link arm member 4
Reference numeral 8 is rotatably provided via a support shaft 25 provided in the main body frame 11 (also used as the support shaft 25 provided in the cultivation container positioning and holding mechanism 21 described above in this embodiment). There is. (See FIGS. 14 to 16)

A drive cylinder 49 is provided on the lower side of the transport mechanism 12, and a tip end portion of a rod 50 provided on the drive cylinder 49 has the link arm member 48.
A piece that is pivotally attached to the end of the link arm member 48 and connected and fixed, and is also attached and fixed to the end side of the link arm member 48 and a cylinder support plate 42 that is reciprocally movable along the guide rod 43. Connecting rod 51 between the portion 42A side
Both ends are pivotally attached and connected and fixed.

A lid opening / closing drive means for reciprocating the lid opening / closing mechanism 29 between the lid closing position and the lid opening position of the cultivation container 18 by the drive cylinder 49 according to the lid opening / closing operation of the lid opening / closing mechanism 29, The liquid inoculum injection mechanism 30 is also used as an inoculum supply driving unit that reciprocates between the position where the liquid seed injection mechanism 30 is separated from the position of the mouth of the cultivation container 18 and the position on the mouth of the cultivation container 18. As a result, the opening operation of the lid opening / closing mechanism 29 and the liquid seed germ injection mechanism 30 are performed.
Is operated in synchronism with the operation of moving the above to the mouth portion of the cultivation container 18, and the closing operation of the lid opening / closing mechanism 29 and the liquid inoculum injection mechanism 30 are separated from the mouth portion position of the cultivation container 18. It is configured to operate in synchronization with the moving operation.

As shown in FIG. 12, FIG. 13, FIG. 17, etc., the culture medium C is filled in the inside of the cultivation container 18, and the surface of the culture medium C filled under pressure in the cultivation container 18 is filled. The floor surface C1, which is a portion, is provided with an inoculation hole C2 in the central portion thereof from the upper surface to the bottom of the culture medium C. This inoculation hole C2 is provided to improve the surrounding of the bacteria in the culture medium C, and improves the air permeability of the culture medium C itself, and when the liquid seed culture B is inoculated, the seed culture spreads to the bottom of the culture medium C. This is done to make it easier.

As a suitable inoculating method and inoculating device for inoculating the surface portion of the culture medium C having the inoculation holes C2 in the culture medium C filled in the inside of the cultivation container 18, the inoculation step by the liquid inoculum injection mechanism 30 is performed. As a process, the step of inoculating the liquid inoculum B mainly on the floor surface C1 position of the culture medium C in the cultivation container 18, and the inoculation hole C2 position formed on the floor surface C1 portion of the culture medium C as the main liquid A liquid inoculum method comprising a step of inoculating inoculum B, which comprises a cultivation container 1
Inoculating liquid inoculum B evenly to the respective surface portions of the floor surface C1 portion which is the surface portion of the culture medium C in 8 and the inoculation hole C2 which is formed by making a depression in a part of the floor surface C1 portion. In this way, since it is possible to favorably promote the growth of hyphae inside the culture medium C by the inoculum, as a device structure for achieving the liquid inoculum inoculation step as described above, the first embodiment Then, the cultivation container 18 in which the liquid inoculum injection mechanism 30 is supplied to the inoculum inoculation region
And the liquid inoculum injection mechanism 3
The raising and lowering device 52 mainly includes the air cylinder 41 that can move 0 toward and away from the cultivation container 18.

As the lifting device 52, in this embodiment, the liquid inoculum injection cylinder body 3 of the liquid inoculum injection mechanism 30 is mounted on the air cylinder 41 via the support plate 40.
7 is detachably attached and fixed, and an air cylinder 41, which constitutes a main part of the lifting device 52, is fixed on a cylinder support plate 42 provided on the main body frame 11 of the inoculation device. In addition, a predetermined position of a vertically moving region of the liquid seed germ injection cylinder body 37 provided with the injection nozzle 36, in this embodiment, the floor surface C of the culture medium C in the cultivation container 18.
A nozzle position detection sensor 53 is arranged mainly at one position in accordance with the position where the liquid inoculum B is sprayed.
6, the nozzle position detection sensor 5
3 is configured to issue a detection signal so as to stop when the injection nozzle 36 reaches a predetermined position.

The air cylinder 41 is used as an elevating device 52 for vertically moving the liquid inoculum injection mechanism 3.
As shown in the operation diagrams of FIG. 24 to FIG. 29, the solenoid valve 5 is supplied with the air pressure from the air supply source P including a compressor or the like.
The liquid inoculum injection mechanism 30 is provided to be movable up and down at a predetermined position by the switching operation of 4. In this case, the air supply source P is connected to the solenoid valve 54 via the fluid pipe 55, and the solenoid valve 54 is connected to the upper and lower portions of the air cylinder 41 via the fluid pipes 56 and 57. Then configured.

In the liquid seed-filled bag 2 and the inoculation device 3 having the above-described structure, a large seed-stock storage tank 1 in which the liquid seed-filled bag 2 is cultivated and stored with the liquid seed B is used.
It is compact and can be easily carried by filling it in aseptic condition into small parts, which makes it easy to carry in, carry out, or install the seed bacteria seller or the seed bacteria purchaser. You can

Further, in carrying in and out of the liquid seed germ-filled bag 2, for example, by storing the filled bag 2 in a hard case and carrying it, the operability can be improved. At this time, the entire circumference of the filling bag 2 may be covered, or in some cases, a frame-shaped case may be used. In short, by suppressing the deformation of the liquid seed germ filling bag 2 having flexibility, Anything can be used as long as it can be easily carried.

The liquid seed germ-filled bag 2 is provided with a feed port 5 that allows the liquid seed germ B in the liquid seed germ-filled bag 2 to be discharged to the outside. It is possible to easily connect the liquid inoculum injection mechanism 30 side formed in the above with the inoculum supply line 4 made of a tube, and it is possible to supply the liquid inoculum B to the inoculation device 3 side in a sterile state. It is a thing. In addition, when the liquid seed germ B is cultivated and stored in the liquid seed germ filling bag 2 in a sterile state from the seed germ storage tank 1, the liquid seed germ B is filled into the liquid seed germ filling bag 2 through the supply port 5. You may do it.

In the normal state of the liquid seed germ-filled bag 2, in this embodiment, the supply port 5 is covered with a cap (not shown) to keep the liquid seed germ B in a sealed state. In such a case, the cap is removed so as to be connectable to the inoculum supply line 4, but in some cases, the supply port 5 may be sealed with a resin film when the liquid inoculum B is filled without providing the cap. It suffices if it is configured so that the film can be broken and communicated with each other when the inoculum supply line 4 is connected.

Next, a series of operations of the inoculation device 3 for the liquid seed bacteria B using the liquid seed bacteria-filled bag 2 in the above-mentioned structure will be described. First, when the power of the inoculation device 3 is turned on, as the initial setting, the liquid inoculum injection cylinder main body 37 of the liquid inoculum injection mechanism 30 is positioned at the uppermost stage (upper limit position) so as not to come into contact with the cultivation container 18. By energizing the solenoid, the solenoid valve 54 is actuated, and pneumatic pressure is applied to the fluid pipe 57 side located below the air cylinder 41, whereby the piston moves upward and the injection nozzle 36 reaches the top dead center position. Return operation. (Refer to FIG. 24) In this state, when a container 19 in which a plurality of cultivation containers 18 with a lid 17 placed on the transport mechanism 12 are vertically and horizontally accommodated is transferred and the container 19 reaches the inoculum inoculation area,
The front end side of the container 19 is positionally held and stopped by the stopper pin 20A provided on the stopper mechanism 20 that moves up and down so as to be retractable with respect to the transport surface of the transport mechanism 12.

In the first embodiment, when the transport mechanism 12 transports the cultivation container 18 in the container 19 to a predetermined inoculum inoculation area, the stopper mechanism 20 regulates the position of the container 19 at a predetermined position. Stop,
After this stop, the cultivation container positioning and holding mechanism 21 is configured to operate. At this time, the cultivation container positioning and holding mechanism 21 preliminarily positions the cultivation containers 18 in the container 19 in horizontal row units, or at the time of inoculation, opens and closes the lid 17 in conjunction with a series of inoculation steps. At this time, the cultivation container 18 is provided so as to be positioned and held.

That is, the cultivation container positioning and holding mechanism 21.
The cultivation container 18 in which the shoulder pressing arm member 22 configured in FIG.
Cultivation container 1 in place by pressing the shoulders of
8 is held, and the predetermined position is maintained while the floating of the cultivation container 18 is suppressed during the opening / closing operation of the lid 17 of the cultivation container 18 in the next step. (See Figures 3 to 9)

When the cultivation container 18 is positioned and fixed by the operation of the cultivation container positioning and holding mechanism 21, the operation of the air cylinder 46 causes the state shown in FIG.
To the state, the lid 17 of the cultivation container 18 positioned and arranged in a horizontal row located in the front row is pressed from the upper surface by the movable clamping member 45, and the fixed clamping member 44 provided on the lower surface and the above. The lid 17 is sandwiched and held between the movable sandwiching member 45 in units of one horizontal row.

Next, after the lid 17 is sandwiched and held while the positioning by the cultivation container positioning and holding mechanism 21 is performed, the link arm member 48 moves the rod 50 with the support shaft 25 as a base point according to the operation of the drive cylinder 49. 16, the lid 17 is rotated to the lid open position by the lid opening / closing mechanism 29 attached to the link arm member 48, as shown in FIG. A connecting rod 51 attached to the end of the link arm member 48 in synchronization with this lid opening operation.
A piece 42A attached and fixed to the cylinder support plate 42 via a guide moves along a guide rod 43 provided on the main body frame 21 side, and the movement of the cylinder support plate 42 causes a liquid inoculum serving as an inoculum supply mechanism. Injection mechanism 30
The four injection nozzles 36 provided in the above are moved to directly above the cultivation container 18 with the lid 17 removed, which is the mouth position of the cultivation container 18. (See Figures 4 and 25)

At the time of this transition, that is, the four injection nozzles 3 provided in the liquid inoculum injection mechanism 30 which is an inoculum supply mechanism.
When 6 is moved right above the mouth position of the cultivation container 18 with the lid 17 removed, the solenoid of the solenoid valve 54 is actuated to operate so that the air pressure on the fluid pipe 56 side is the upper side of the air cylinder 41. In addition, the piston of the air cylinder 41 operates downward, and the liquid seed germ injection cylinder main body 37 side of the liquid seed germ injection mechanism 30 attached to the air cylinder 41 is positioned at the mouth side position of the cultivation container 18 together with the injection nozzle 36. Is placed close to or inserted into the mouth. (See Figures 5 and 25)

At this time, when the jet nozzle 36 descends, the jet nozzle 3 is detected by the nozzle position detection sensor 53.
When the passage of 6 is detected and the detection signal causes the solenoid of the solenoid valve 54 to be de-energized, the solenoid valve 54 moves to the neutral position, so that all of the air supply port and the exhaust port are blocked, Air cylinder 4
The movement of the air cylinder 41 is stopped by shutting off the supply / exhaust air of No. 1. (See Figure 26)

When the air cylinder 41 is stopped,
When the lid 17 of the cultivation container 18 is opened by the lid opening / closing mechanism 29, the liquid inoculum B is sprayed mainly on the floor surface C1 of the culture medium C in the cultivation container 18 in a horizontal row in which the lid 17 is opened. By spraying each through
The floor surface C1 portion of the culture medium C in the cultivation container 18 in horizontal row units is inoculated. (See FIGS. 6, 12, 20, and 27)

After injecting a predetermined amount of the liquid inoculum B to the floor surface C1 of the culture medium C in the cultivation container 18 in a horizontal row unit by the injection nozzle 36, the solenoid of the solenoid valve 54 is energized, and then the air cylinder is energized. By applying pneumatic pressure to the fluid pipe 56 side connected to the upper side of 41, the piston of the air cylinder 41, which constitutes the main part of the lifting device 52, operates further downward and is attached to the air cylinder 41. The liquid inoculum injection mechanism main body 37 side of the liquid inoculum injection mechanism 30 is further inserted and arranged in the mouth of the cultivation container 18 in horizontal row units together with the injection nozzle 36, and stops at the lowermost position (lower limit position). (See FIGS. 7 and 28)

At this lower limit position, the cultivation container 18
Mainly at the position of the inoculation hole C2 formed in the floor surface C1 portion of the culture medium C inside, by spraying a predetermined amount of the liquid inoculum B through the injection nozzle 36 of the liquid inoculum injection mechanism 30, a horizontal line Culture medium C in the cultivation container 18 in which the unit is one set
The inoculation hole C2 part of is mainly inoculated. (Figs. 8 and 1
3, see FIG. 21, FIG. 29, etc.)

After injecting a predetermined amount of the liquid inoculum B by the injection nozzle 36 in this way, the solenoid of the solenoid valve 54 is switched to energize to apply an air pressure to the fluid pipe 57 side to the lower side of the air cylinder 41. As a result, due to the return operation of the air cylinder 41, the injection nozzle 36 provided in the liquid seed germ injection mechanism 30 separates from the mouth position of each cultivation container 18 and returns to the initial position, that is, upward.

Thereafter, the injection operation of the injection nozzle 36 is retreated by moving back from the position of the cultivation container 18 by the return operation of the rod 50 of the drive cylinder 49, and at the same time, the liquid inoculum B is inoculated by the rotation return operation of the lid opening / closing mechanism 29. The lid 17 of the cultivating container 18 in the unit of one horizontal row is fitted.

At this time, the cultivation container positioning and holding mechanism 2 for holding the shoulders of the cultivation containers 18 adjacent to each other in the cultivation containers 18 in a horizontal row transferred to the inoculum contact area.
Since 1 holds the cultivation containers 18 in units of one horizontal row while suppressing the positional deviation, the opening and closing operation of the lid 17 can be favorably performed, and the inoculation work of the inoculum B is also favorably performed.

Therefore, while the cultivation container positioning and holding mechanism 21 presses and holds the cultivation container 18 in horizontal row units in advance, the lid opening / closing mechanism 29 holds the cultivation container 1 in horizontal row units.
By opening and closing the lid 17 of No. 8 and sequentially operating a series of steps by the operation of the elevating device 52 that enables the liquid seed culture injection mechanism 30, which is the seed culture supply mechanism, to move toward and away from the cultivation container 18, Container 19 in the inoculation area
The cultivation container 18 in the front row with respect to the transfer direction can satisfactorily inoculate the entire surface of the culture medium C in the cultivation container 18 in the horizontal row unit, that is, the floor surface C1 site and the inoculation hole C2 site of the culture medium C. .

When a series of inoculation steps based on the cultivation container 18 in the front row is completed, the stopper pin 20A that stopped and holds the front end of the container 19 once descends downward via the air cylinder 20B. The container 19 is transferred in the carry-out direction by the upward return operation and the transfer mechanism 12 operation. Due to the transfer operation of the container 19, the stopper pin 20A that initially holds the front end portion of the container 19 also tries to return to the upper position, but the stopper pin 20A that has been stopped and held until now due to the transport operation of the container 19 is performed. Has its upper end abutted against the bottom of the container 19. However, since the upward biasing force of the stopper pin 20A is set to be weak, the container 19 can be smoothly transferred without hindering the transfer, and is provided in the next row of the stopper machine 20. The front end side of the container 19 is stopped and held by another stopper pin 20A. (Fig. 2
(See 2)

When the container 19 is stopped, as described above, the lid opening / closing mechanism 29, the liquid inoculum inoculation mechanism 30, and the lifting / lowering device 52 perform a series of inoculation steps based on the second row of the cultivation container 18. By being given a second
The cultivation container 18 in the container 19 can be inoculated as shown in FIG. 21, and subsequently, the inoculation process is performed row by row in the order of the third and fourth rows. Can be inoculated.

All the cultivation containers 18 in the container 19
When the inoculation step of the above is completed, the stopper pin 20A of the stopper mechanism 20 is moved to the air cylinder 2 as shown in FIG.
The container 19 that temporarily lowers through 0B and then returns to the upper position, and the transport mechanism 12 transfers the container 19 containing the inoculated cultivation container 18 to the unloading side, and newly inoculates the cultivation container. By supplying the container 19 accommodating 18 from the carry-in side of the transport mechanism 12, the inoculation work can be continuously performed.

Further, the opening operation of the lid 17 by the lid opening / closing mechanism 29 is sequentially performed from the front row position to the cultivation container 18 in horizontal row units, and the liquid inoculum injection mechanism 30 and the elevating device 52 which are inoculum supply mechanisms are used. The cultivation container 18 housed in the container 19 by repeating the operation of inoculating the liquid inoculum B and the closing operation of the lid 17 by the lid opening / closing mechanism 29.
Can be continuously inoculated in a horizontal row unit, and the inoculation work efficiency of the liquid inoculum B can be increased, and the horizontal opening / closing operation of the lid 17 of the cultivation container 18 and the inoculation can be performed in a row unit. Cultivation container 1 for performing operations
The opening time of the lid 17 of No. 8 can be shortened as much as possible, and invasion of various bacteria can be suppressed.

By the way, in the first embodiment of the present invention, when the liquid seed germ inoculating device is in operation, the inside of the flexible liquid seed germ-filled bag 2 filled with the liquid seed germ B in an aseptic state is a compressor. The air pressure in the pressure vessel 7 is increased by applying the air pressure by the liquid inoculum pressurizing means 8 including the liquid inoculum, and the liquid inoculum-filled bag 2 itself is pressurized from the outside by the increased air pressure. Since the internal pressure inside the seed-filled bag 2 is constantly increased, the liquid seed-filled bag 2 in which the internal pressure is increased
Liquid inoculum B pressure-fed from the inoculum supply line 4 from
The liquid is supplied to the seed supply port 37A provided in the liquid seed injection cylinder main body 37 and flows into the hollow fluid passage 37B under pressure. (See FIGS. 2 and 17)

At the time of this inflow, when the liquid seed germ injection mechanism 30 is not in operation except during the time when the liquid seed germ B is sprayed by the jet nozzle 36 and inoculated, the valve body 31A of the injection opening / closing valve 31 is the liquid seed germ. By maintaining contact with the valve seat 37C provided in the hollow fluid passage 37B of the injection cylinder main body 37 and maintaining a hermetically closed state, the liquid inoculum B is attached to the injection nozzle 36 side attached to the tip side of the hollow fluid passage 37B. Since it is not supplied, the liquid inoculum B is not sprayed.

That is, the liquid seed germ injection cylinder body 37
In this embodiment, as a means for maintaining a closed state of the valve seat 37C of the hollow fluid passage 37B provided by the valve body 31A of the injection opening / closing valve 31, the gas pressurizing means 32 including a compressor is used. The supplied air is pressure-fed through the gas supply pipe line 34 and is guided through the filter 33 to the gas supply port 37D provided on the rear end side of the liquid inoculum cylinder body 37.
By closing the electromagnetic valve 35 disposed between the gas supply port 37D and the gas supply port 37D, the gas whose air pressure is increased is blocked without being transmitted to the piston shaft 31B side of the injection opening / closing valve 31. For this reason, the valve element 37A of the hollow fluid passage 37B provided in the liquid inoculum injection cylinder main body 37 is formed by the piston spring 37C interposed between the large diameter portion provided in the piston shaft 31B and the liquid inoculum injection cylinder main body 37. The valve seat 37C is always elastically urged, and the elastically urging force maintains the shutoff state without supplying the liquid inoculum B. (See Figure 17)

When the liquid inoculum B is injected (see FIGS. 6, 8, 12, 13, 18, 18, 19, 27 and 29), as shown in FIGS. By opening the solenoid valve 35, the valve body 31A of the injection opening / closing valve 31 is maintained in the open state with respect to the valve seat 37C of the hollow fluid passage 37B provided in the liquid seed germ injection cylinder body 37. That is, by opening the solenoid valve 35, the air pressure-fed by the gas pressurizing means 32 including a compressor or the like is supplied to the rear of the liquid seed germ injection cylinder main body 37 via the filter 33. It is guided to the gas supply port 37D provided on the end side. The air pressurized from the gas supply port 37D is supplied to the piston shaft 31B and the liquid seed germ injection cylinder body 37.
A diaphragm 3 made of a flexible material and disposed between
Since it is directly added to 1D, the diaphragm 31D is pressed to bend and move. Diaphragm 31D due to its bending
The piston shaft 31B moves along with the movement of the piston shaft 31B, and the valve body 31A provided at the tip of the piston shaft 31B moves away from the valve seat 37C of the hollow fluid passage 37B provided in the liquid inoculum injection cylinder body 37. Thus, the liquid seed germ B is sprayed from the injection nozzle 36 by opening the valve body 31A of the injection opening / closing valve 31 from the closed state.

At this time, the opening time of the electromagnetic valve 35 is set to a predetermined time, or the electromagnetic valve 35 is shut off when the flow rate of the liquid inoculum B reaches a predetermined amount, so that the inoculation amount of the liquid inoculum B can be adjusted appropriately. Can be set to state.

In other words, in this embodiment, the injection opening / closing valve 3 is switched in accordance with the switching operation of the electromagnetic valve 35, which is cut off or opened.
The valve opening / closing drive control means 13A for setting the flow rate or the inoculation time for inoculating the liquid inoculum B by switching 1 is provided on the operation panel 13 so that the capacity or form of the culture medium C in the culture container 18 or the culture medium C can be obtained. Valve opening / closing drive control means 13A for adjusting the inoculation filling amount of the liquid inoculum B according to the medium of C
The liquid inoculum C can be maintained at a substantially constant amount without variation, and stable inoculation process work can be performed.

Further, in order to keep the space between the hollow fluid passage 37B side provided on the liquid seed injection cylinder body 37 and the gas supply port 37D provided at the rear end side of the liquid seed injection cylinder body 37 watertight, By disposing a diaphragm 31D made of a flexible material such as synthetic rubber, which is an airtight member, between the shaft 31B and the liquid seed injection cylinder main body 37, pressure is applied for switching operation of the injection opening / closing valve 31. The air can be blocked without being mixed into the hollow fluid passage 37B, and the diaphragm 3
By opening the valve body 31A in synchronism with the movement of 1D, the liquid inoculum B pressurized by the liquid inoculum pressurizing means 8 is injected through the injection nozzle 36 provided on the tip side of the liquid inoculum injection cylinder body 37. The culture medium C of the cultivation container 18 can be sprayed and inoculated, and there is also an effect that invasion of various bacteria can be suppressed as much as possible at the time of inoculation.

Further, 4 provided in the liquid inoculum injection mechanism 30
Although not shown, the liquid seed germ injection cylinder main body 37 having the individual injection nozzles 36 is detachably fixed to a plate-shaped nozzle attachment member 39 by fixing screws via nozzle fixing members 38, and this nozzle attachment is also performed. Since the member 39 is detachably attached and fixed to the support plate 40 on the body frame 11 side of the inoculation device, it can be easily removed. That is, the nozzle mounting member 39 and the nozzle mounting member 39 are fixed together by the notch groove 39A provided in the nozzle mounting member 39 by the fixing screw 40A, and the supporting plate 40 is loosened by loosening the fixing screw R. The nozzle mounting member 39 can be easily removed along the cutout groove 39A with respect to the 40 side.
Since the individual liquid inoculum injection mechanism 30 can be attached and detached as a single unit, maintenance work such as disassembly and assembly work of each component, sterilization treatment, disinfection work, and cleaning work can be easily performed. It is also possible. Moreover, when disassembling the liquid inoculum injection mechanism 30, the inoculum supply pipe 4 connected to the supply port 37A of the liquid inoculum injection cylinder body 37 is removed, and the liquid inoculum injection mechanism 30 and the liquid inoculum injection cylinder body 37 are injected. By disinfecting the nozzle 36 and the injection opening / closing valve 31 with alcohol, it is possible to easily carry out the bacteria treatment, and there is also an effect that the maintenance management of each part and the like can be carried out easily. For maintenance, inspection or sterilization,
Since the injection nozzle 36, the inoculum supply line 4, the gas supply line 34, etc. on the side that supplies the liquid inoculum B need to perform parts replacement work, cleaning work, and disinfection work (if necessary), fixing screws or It is desirable that the attachment / detachment and replacement work can be easily performed by a fixing means such as a detachment knob or a bayonet connection.

When installing the liquid inoculum injection mechanism 30,
By arranging and setting the set position of the injection nozzle 36 so as to be adjustable according to the height dimension and size of the cultivation container 18, the positional relationship stored in the container 19, and the like, the liquid inoculum B in the cultivation container 18 is formed. It is possible to satisfactorily spray and inoculate.

The liquid seed bacteria B in the liquid seed filling bag 2
When there is no longer, the liquid seed germ filling bag 2 can be replaced by switching and connecting a tube or pipe to another liquid seed germ filling bag 2 filled with the liquid seed germ B. In some cases, the liquid seed germ filling bag 2 can be replaced. It is also possible to prepare a plurality of liquid seed bacteria-filled bags 2 and to easily perform the replacement work by switching the open / close valve when the liquid seed bacteria B in the liquid seed bacteria-filled bag 2 are exhausted. Liquid inoculum B
Since the liquid seed germ-filled bag 2 is made of a flexible material made of synthetic resin after being used up, it is not bulky and is easy to carry.

Further, the injection nozzle 36 attached and fixed to the tip side of the hollow fluid passage 3B of the liquid inoculum injection cylinder main body 37 is set so as to have a predetermined injection pattern when the liquid inoculum B is sprayed into the inside thereof. Nozzle tip 36
By disposing A, the injection port 36 of the injection nozzle 36
The liquid inoculum B pressurized from B is the culture medium C of the cultivation container 18.
Can be sprayed toward, and the step of inoculating mainly the floor surface C1 portion of the surface portion of the culture medium C filled in the cultivation container 18 and the step of mainly inoculating the portion of the inoculation hole C2 The inoculum spreads almost uniformly over the entire surface of the culture medium C, which makes it possible to promote the growth of hyphae by the inoculum.

30 to 33 show the second embodiment of the present invention, and FIG. 30 is similar to the above-mentioned first embodiment.
Liquid inoculum B from the inoculum storage tank 1 is subdivided and sealed in a liquid inoculum filling bag 2 while maintaining a sterile condition. The subdivided aseptic liquid seed culture filling bag 2 is used for inoculation of the seed culture via the seed supply pipe line 4 made of a flexible tube when inoculating the seed culture.
It is connected to the liquid inoculum injection mechanism 30 side.

The liquid seed germ-filled bag 2 of the second embodiment is provided so as to be housed in a container 7A which is a hard outer box as shown in FIG. On the side of the container 7A, the liquid seed germ B is injected from the liquid seed germ filling bag 2 through the seed feed line 4 to the liquid seed germ injection mechanism 3 of the inoculation device 3.
In order to pressurize and supply the liquid inoculum to the 0 side, the liquid inoculum pressurizing means 8A including a pressing plate for pressing the liquid inoculum filling bag 2 from the upper side of the container 7A is provided, and the liquid inoculum comprising the pressing plate is provided. Although not shown, the pressurizing means 8A is configured so that a predetermined pressurizing force is applied from the outside of the liquid seed germ-filled bag 2 by a pressing means such as a spring or a cylinder.

As the inoculation device 3, the inoculation device 3 is used.
A transport mechanism 12 including a conveyor is provided over substantially the entire length of the main body frame 11, and the transport mechanism 12 can be started and stopped by operating a switch on the operation panel 13. In this embodiment, as the cultivation container 18A, a container is formed by a rectangular packaging bag as shown in FIG. 31, and the cultivation container 18A made of this rectangular packaging bag is used.
The culture medium C such as sawdust and rice bran is pressed and filled inside, and the floor surface C1 which is the surface portion of the culture medium C is directed from the upper surface side to the bottom of the culture medium C at a predetermined interval. Two inoculation holes C2 are provided. The cultivation container 18A made of this synthetic resin packaging bag is placed on the transport mechanism 12 at the transfer start side, and the transport mechanism 12
The cultivation container 18A consisting of the packaging bag is transferred to the transfer end side from the carry-in side to the inoculation area for inoculating the liquid inoculum B by starting the Are configured to be intermittently transferred. In this case, as means for stopping the cultivation container 18A sent through the transport mechanism 12 when the cultivation container 18A reaches the proper inoculum inoculation area, in this embodiment, a predetermined value of the main body frame 11 of the inoculation device 3 is used. A detection switch S serving as a cultivation container detecting means is provided at the location.

The cultivation container 18A consisting of a packaging bag stopped in the inoculum inoculation area is supplied to the transport mechanism 12 of the inoculation device 3 with the packaging bag opening in advance.
Alternatively, the bag mouth of the packaging bag is opened by a bag mouth opening mechanism (not shown) incorporated in the inoculation device 3, and the bag is sent to the seed inoculation area.

In the seed inoculation area of the inoculation device 3,
Cultivation container 18A in the rectangular packaging bag that is conveyed
Two liquid inoculum injection mechanisms 30 are provided according to the position and the number of the inoculation holes C2 in the liquid inoculation hole C2. It is attached and fixed to an elevating device 52 that can move toward and away from the cultivation container 18A formed of the rectangular packaging bag. An air cylinder 41A is used as a drive means that constitutes a main part of the lifting device 52 as in the first embodiment.
The liquid inoculum injection cylinder main bodies 37 of the two liquid inoculum injection mechanisms 30 are detachably attached and fixed to the main body frame 11 of the inoculation device 3 by the support plate 40A. It is fixed on the provided cylinder support plate 42B.

Further, a predetermined position of a vertically moving region (operating region) of the liquid seed germ injection cylinder body 37 equipped with the injection nozzle 36, in this embodiment, the culture medium C in the cultivation container 18A
The nozzle position detection sensor 53A is arranged mainly at one floor surface C of the liquid sprayer and is positioned at a position where the liquid inoculum B is sprayed. When the jet nozzle 36 descends, the jet nozzle 36 reaches the predetermined position when the jet nozzle 36 reaches a predetermined position. Nozzle position detection sensor 5
3A works, and a predetermined amount of the liquid inoculum B set during a predetermined time is sprayed from the injection nozzle 36. At this time, since the piston of the air cylinder 18A, which is the lifting device 52, continuously moves downward, the cultivation container 18
In addition to the floor surface C1 of the culture medium C in A, the injection nozzle 3
With the downward movement of 6, the inoculation holes C2 in the floor C1 of the culture medium C are continuously inoculated.

The air cylinder 41A, which is the elevating device 52 for vertically moving the liquid seed germ injection mechanism 30 in the second embodiment, changes the pneumatic pressure from the air supply source P by switching the solenoid valve 54A. It is configured such that it can be moved up and down between the upper limit position and the lower limit position. In this case, the air supply source P is connected to the solenoid valve 54A via a fluid conduit 55A, and the solenoid valve 54A is connected to the upper and lower portions of the air cylinder 41A via fluid conduits 56A and 57A. .

The liquid inoculum B pressurized and supplied by the liquid inoculum pressurizing means 8A is sprayed and inoculated by the liquid inoculum injection mechanism 30 onto the culture medium C in the cultivation container 18A. Provided injection on-off valve 31
Is operated to supply and block the liquid seed bacteria B pressure-fed from the liquid seed bacteria filling bag 2 through the seed bacteria supply pipe line 4.

In this embodiment, the liquid inoculum injection mechanism 3 is used.
As a means for reciprocating the injection opening / closing valve 31 for supplying and shutting off the liquid seed germ B provided at 0, an electromagnetic driving means 31A including an electromagnet and an armature is provided, and is linked with the operation of the electromagnetic driving means 31A. Valve opening / closing drive control means 1 for switching the injection opening / closing valve 31 to set the time for inoculating the liquid inoculum B or the flow rate for inoculation.
3A is provided on the operation panel 13. Although not shown, the shaft provided with the valve element provided in the injection opening / closing valve 31 is configured to reciprocate by the operation of the electromagnetic drive means 31A, which is not shown. In this case, since the reciprocating operation of the injection opening / closing valve 31 is performed by the electromagnetic driving means 31A, there is no need to use a pneumatic driving means, and therefore air is mixed when the liquid inoculum injection mechanism 30 injects the liquid inoculum B. Without doing so, as a result, the invasion of various bacteria can be further prevented.

In the liquid seed-filled bag 2 and the inoculation device 3 having the above-described structure, the large seed-stock storage tank 1 in which the liquid seed B is cultured and stored is sterilized in a liquid state as in the first embodiment described above. By filling the seed germ filling bag 2 in small portions, the liquid seed germ filling bag 2 can be made compact and easy to carry, whereby the liquid seed germ filling bag 2 can be carried in, carried out, or installed during inoculation. Work can be performed more easily than in the past.

Next, a series of operations of the liquid seed culture inoculating device in the second embodiment will be described. After the power is turned on by operating the switch of the operation panel 13, the cultivation container 18A made of a rectangular packaging bag is placed on the transport mechanism 12 and transferred, and when the cultivation container 18A reaches the inoculum inoculation area, the cultivation container detection means is used. The detection switch S is operated and the transport mechanism 12 is stopped. At this stop position, that is, in the seed inoculation area, the cultivation container 18A made of the rectangular packaging bag is fed immediately below each injection nozzle 36 of the liquid seed injection mechanism 30 and stopped.

On the basis of the detection signal of the detection switch S, the solenoid of the solenoid valve 54A is actuated by energization to apply air pressure to the fluid pipe 55A side above the air cylinder 41A, and to move the air in the lifting device 52. Cylinder 41A
Piston of the air cylinder 4 operates downward,
Cultivation container 1 in which two cylinder bodies 37 attached to 1A are formed of rectangular packaging bags together with injection nozzles 36
8A is approached to the bag mouth portion side position or inserted and arranged in the bag mouth portion.

At this time, at the time of the lowering operation of the injection nozzle 36, a predetermined position of the vertically moving region (operation area) of the liquid seed germ injection cylinder body 37 equipped with the injection nozzle 36,
In this embodiment, the nozzle position detection sensor 53A is arranged mainly at the floor surface C1 position of the culture medium C in the cultivation container 18A made of a packaging bag in accordance with the position where the liquid inoculum B is sprayed. Injection nozzle 36
When it is detected that the liquid has passed a predetermined position, the electromagnetic drive means 31A is operated based on the detection signal to open the injection opening / closing valve 31 for a predetermined time, and by this opening, a predetermined amount of liquid Inoculum B is sprayed. At this time, since the piston of the air cylinder 41A, which is the lifting device 52, continuously moves downward, the cultivation container 1
In addition to the floor C1 of the culture medium C in 8A, the inoculation holes C2 formed in the floor C1 of the culture medium C are continuously sprayed along with the downward movement of the injection nozzle 36. Be inoculated.

Therefore, as the spray nozzle 36 moves up and down, the liquid inoculum B is continuously sprayed during a predetermined stroke (spray area) due to the vertical movement of the spray nozzle 36, whereby the floor surface C1 of the culture medium C is located at a location C1. It is possible to inoculate all of the inoculation holes C2 places at once.

Also, the culture medium C filled in the cultivation container 18
By continuously performing the step of inoculating mainly on the floor surface C1 part of the surface part of the plant and the step of inoculating mainly the part of the inoculation hole C2, the inoculum can be evenly spread over the entire surface of the culture medium C. As a result, it becomes possible to promote the growth of hyphae by the inoculum.

The present invention is not limited to the above-mentioned embodiments, and various modifications can be made within the scope of the gist of the present invention. In the first and second embodiments, the liquid inoculum filling is performed. Although the bag 2 is made of a synthetic resin bag, a packaging bag made of a cloth bag such as Japanese Patent Laid-Open No. 8-268491 may be used, and the liquid seed-filled bag 2 is suspended. A type may be adopted, and a back-in-box type packaging bag may be used in consideration of transportation work.

Further, the liquid seed bacteria B in the bag 2 filled with the liquid seed bacteria.
In the first embodiment, the liquid seed germ filling bag 2 is housed in the pressurizing container 7 as a means for sending the liquid to the liquid seed germ injection mechanism 30 side of the inoculation device 3, and the internal pressure in the pressurizing container 7 is increased to form the liquid. The inoculum-filling bag 2 is pressurized to send out the liquid inoculum B through the inoculum supply conduit 4, or in the second embodiment, the liquid-inoculum pressurizing means 8A, which is a pressing plate, is provided directly outside the liquid inoculum-filling bag 2. Although it is configured to be pressed and sent out through the seed culture supply line 4, it is supplied, for example, on the transportation route of the seed culture supply pipe 4 between the liquid seed culture filling bag 2 and the liquid seed injection mechanism 30. Liquid inoculum B filled with liquid inoculum B while pressurizing via a pump or the like 2
May be supplied to the liquid inoculum injection mechanism 30 side.

Further, the liquid seed filling bag 2 is integrally provided with a supply port 5 which is connected to the seed supply supply line 4 to guide the supply of the liquid seed B to the outside. By forming a metal material,
This is advantageous because there is no fear of damaging the supply port 5 when cleaning it, or when performing disinfection work by alcohol spray or flame disinfection.

Further, in the first embodiment, as the procedure of the inoculation process by the liquid inoculum injection mechanism 30, first, the cultivation container 1
Mainly at the floor surface C1 of the culture medium C in 8, the spray nozzle 36 of the liquid inoculum injection mechanism 30 is stopped and sprayed at a predetermined position, and then the inoculation hole formed in the floor surface C1 portion of the culture medium C. Although the injection nozzle 36 of the liquid inoculum injection mechanism 30 was stopped at a predetermined position and sprayed mainly at the C2 position, the raising and lowering device 5 was temporarily used.
After moving the injection nozzle 36 to the lower limit position by the operation of 2, first inoculate the inoculation hole C2 portion of the culture medium C, then raise the injection nozzle 36 and stop the injection nozzle 36 at a predetermined position. After that, the floor surface C1 of the culture medium C may be inoculated, and in the second embodiment, the packaging is performed in the continuous downward movement (downward movement) of the piston of the air cylinder 41A. In addition to the floor surface C1 of the culture medium C in the cultivation container 18A made of a bag, the inoculation hole C2
Although the site was continuously inoculated, the inoculation hole C2 is similarly provided during the upward movement of the air cylinder 41A.
It is also possible to continuously inoculate the location and the floor surface C1 location of the culture medium C, and in the continuous downward movement of the piston provided in the air cylinder 41A that is the lifting device 52. The same effect can be obtained by intermittently spraying the floor C1 position of the culture medium C and the position of the inoculation hole C2 at the predetermined position by spraying the spray nozzle 36 of the liquid seed bacteria spraying mechanism 30 respectively. Is obtained.

In each of the embodiments, the air cylinders 41, 4 are used as driving means for the lifting device 52 so that the liquid seed germ injection mechanism 30 approaches and separates from the cultivation containers 18, 18A.
Although it is configured to be reciprocally movable in the vertical direction by 1A, it may be operated by using a driving mechanism such as a cam mechanism or a motor as the driving mechanism.

In the first embodiment, the culture medium C is housed in the culture container 18 consisting of a culture bottle, and one inoculation hole C2 is provided at approximately the center of the floor surface C1 which is the surface portion of the culture medium C. As an example, in the second embodiment, the culture medium C is stored in a cultivation container 18A made of a rectangular packaging bag, and two inoculums are placed on the floor surface C1 which is the surface portion of the culture medium C at a predetermined interval. Although the description has been given taking the case where the holes C2 are provided as an example, the number of the inoculation holes C2 and the size and depth of the depressions may be appropriately set, and in some cases, a small inoculum for ventilation. A hole C2 may be additionally provided, and the liquid inoculum injection mechanism 3 is adapted to the surface area and surface shape of the culture medium C.
The injection form and spray amount by the injection nozzle 36 provided at 0, the number and arrangement of the injection nozzles 36 and the like may be set appropriately.

Further, the injection nozzle 3 is adapted to the surface area and surface shape of the culture medium C or the size and depth of the inoculation hole C2.
By setting the injection flow rate and the injection position of the liquid inoculum B according to 6, the flow rate distribution by the injection of the liquid inoculum B inoculated on the surface portion of the culture medium C filled in the cultivation containers 18 and 18A is set to an appropriate amount. As a result, the liquid inoculum B can be satisfactorily sprayed and inoculated into the cultivation containers 18 and 18A.

In the embodiment, the injection opening / closing valve 31
The operation is switched by pneumatic control, electromagnetic control, or the like, but it may be switched by hydraulic control or reciprocating movement by a cam mechanism.

When the liquid seed culture spraying mechanism 30 which is a seed supply mechanism is moved back and forth between the position separated from the mouth of the cultivation container 18 and the position above the mouth of the cultivation container 18, the first embodiment is carried out. In the example, the injection nozzle 36 provided in the liquid seed germ injection mechanism 30 is disposed so as to be horizontally reciprocable along the guide rod 43 by the operation of the drive cylinder 49 so as not to interfere with the operation of the lid opening / closing mechanism 29. Along with the position directly above the cultivation container 18, the cultivation container 18
The air cylinder 41 was provided so as to be able to reciprocate in the vertical direction so as to move toward and away from the position. However, in some cases, the guide rod 43 is omitted, and the position of the inoculum inoculation region in horizontal row units of the cultivation container 18 is arranged. Cultivation container 1 according to the relationship
It is also possible to adopt only the structure of the air cylinder 41 that moves the liquid seed germ supply mechanism 30 up and down at a position directly above 8, and at that time, it is set so as not to hinder the operation of the lid opening / closing mechanism 29. I wish I had it.

Further, in the above-described embodiment, the lid opening / closing drive means for reciprocating the lid opening / closing mechanism 29 between the lid closing position and the lid opening position of the cultivation container 18, and the liquid seed germ injection as the seed feeding mechanism. Although the mechanism 30 is constituted by the drive cylinder 49, it also serves as a liquid seed culture supply driving means for reciprocating between the position where the mechanism 30 is separated from the position of the mouth of the cultivation container 18 and the position on the mouth of the cultivation container 18. The driving means may be individually provided, or the driving means may be a driving mechanism such as a cam mechanism or a motor.

[0120]

As described in detail above, according to the inventions of claims 1 to 3, the flexibility is obtained by subdividing and filling the liquid inoculum in a sterile state from the inoculum storage tank in which the liquid inoculum is cultured and stored. Since it is a liquid inoculum filling bag that has, it is not necessary to carry a seed inoculum storage tank that stores and stores a heavy liquid inoculum, and the operability can be improved by filling the liquid inoculum into a compact and easy-to-carry liquid inoculum bag. Since the liquid inoculum is sealed and packed in a good condition in the sterilized state, the inoculation work as the next step can be easily performed. Further, the liquid seed bacteria-filled bag is provided with a supply port that allows the liquid seed bacteria in the liquid seed bacteria-filled bag to be discharged to the outside, so that the liquid seed bacteria can be easily supplied to the outside from the supply port. Is possible. In addition, by using the liquid seed germ-filled bag as an inner bag and covering the outer side with an outer box made of a hard material, it is possible to protect the filling bag by partially covering the outer side of the filling bag while keeping it in shape, so that it can be carried. Can be done well.

Further, in the invention of claim 4, there is provided a pouring step of pressurizing and supplying the liquid inoculum from the bag in which the liquid inoculum is subdivided into a sterile state through the inoculum supply line to the liquid inoculum injection mechanism. The method for inoculating a liquid inoculum comprising a flow rate setting step in which a predetermined amount of the liquid inoculum is pressurized and sprayed from a spray nozzle provided in the liquid inoculum injection mechanism to a culture medium of a cultivation container to perform a switching operation. As it is subdivided by the seed-filled bag, the operability is good,
Supplying liquid inoculum to the liquid inoculum injection mechanism side while pressurizing,
In the flow rate setting step, it is possible to spray while injecting a predetermined amount of liquid inoculum from the injection nozzle provided in the liquid inoculum injection mechanism, thereby inoculating and inoculating the culture medium of the cultivation container with a predetermined amount of liquid inoculum. You can

Further, in the invention of claim 5, the step of inoculating the liquid inoculum mainly with the floor surface position of the culture medium in the cultivation container at the time of inoculation by the injection nozzle provided in the liquid inoculum injection mechanism, Since it is a method of inoculating liquid inoculum mainly consisting of inoculating the liquid inoculum mainly on the position of the inoculation hole drilled in the floor part, the floor part which is the surface part of the culture medium in the cultivation container and its floor It becomes possible to inoculate the liquid inoculum evenly on each surface part of the inoculation hole that is formed by denting a part of the surface part, which can favorably promote the growth of hyphae on the culture medium by the inoculum. It will be possible.

Further, according to the invention of claim 6, in the inoculation device for inoculating the culture medium in the cultivation container with the inoculum, the liquid inoculum is subdivided and filled in a sterilized state from the inoculum storage tank in which the liquid inoculum is cultured and stored. A liquid inoculum filling bag having flexibility, an inoculum supply line for supplying a liquid inoculum from this liquid inoculum fill bag, and a liquid inoculum is pressurized in the transport route of the liquid inoculum fill bag or the inoculum supply line. Liquid inoculum pressurizing and supplying means for delivering the liquid inoculum, a liquid inoculum injection mechanism for spraying and inoculating the liquid inoculum pressurized and supplied from the liquid inoculum pressurizing and supplying means to the culture medium of the cultivation container, and this liquid inoculum injection Provided in the mechanism,
Since the liquid inoculum inoculation device comprises a spray on-off valve for supplying and shutting off the liquid inoculum that has been pressure-fed from the liquid inoculum filling bag through the inoculum supply line, it is compact and easy to carry. By filling the bag with liquid seeds, the operability can be improved and the bag can be connected to the seed supply line provided in the inoculation device, and the liquid seeds can be directly pressurized by the liquid seed pressurizing supply means. It is supplied to the inoculum injection mechanism side and the injection on / off valve is operated, so that the amount of inoculum supplied from the liquid inoculum filling bag can be sent out at a substantially constant amount, whereby a predetermined amount of inoculum is sprayed onto the culture medium of the cultivation container. Is what you can do.

According to a seventh aspect of the invention, there is provided a liquid inoculum inoculation device comprising valve opening / closing drive control means for switching the spray on / off valve to set a flow rate or inoculation time for inoculating the liquid inoculum. Adjusting and setting the inoculum filling amount of the inoculum by the valve opening / closing drive control means according to the injection form of the inoculum injection mechanism and the diameter of the injection port, or according to the capacity and form of the culture medium in the cultivation container or the medium of the culture medium In addition, it is possible to maintain a substantially constant amount while setting the filling amount of the inoculum without variation and to perform stable inoculation process work.

Further, in the invention of claim 8, the liquid inoculum injection mechanism is provided with an elevating mechanism capable of moving toward and away from the cultivation container, and the liquid inoculates at a predetermined operating position by the elevating mechanism. While inoculating by spraying a liquid inoculum mainly on the floor surface position of the culture medium in the cultivation container by the inoculum injection mechanism, the liquid inoculum mainly on the inoculation hole position perforated in the floor surface portion of the culture medium Since it is a liquid inoculum inoculating device configured to be sprayed and inoculated, at a predetermined operating position by the elevating device, the floor surface portion which is the surface portion of the culture medium in the cultivation container and the floor surface portion thereof. The liquid inoculum can be uniformly inoculated by the liquid inoculum injection mechanism into the respective surface portions of the inoculation hole formed by denting a part of the inoculum.

[0126] According to the invention of claim 9, in claim 6 to claim 8, the cultivation container is formed of a packaging bag made of synthetic resin or the like, so that the bag mouth side of the packaging bag serves as a culture medium. A predetermined amount of liquid inoculum can be inoculated toward the target.

According to a tenth aspect of the invention, in the sixth to eighth aspects, the cultivation container is formed by a cultivation bottle with a lid made of synthetic resin or the like, and a plurality of the cultivation bottles are vertically and horizontally aligned. A transport mechanism that supplies the stored container from the carry-in side and transports the container to the carry-out side while intermittently transporting the culturing bottle, and a lid for the culturing bottle in the container that has been transported to the seed inoculation position by this transport mechanism. A lid opening / closing mechanism for sequentially opening and closing a predetermined number of pieces, and a culture medium in the cultivation bottle with the lid opened and opened according to the number of cultivation bottles opened and closed by the lid opening / closing mechanism. A liquid inoculum inoculation device comprising the liquid inoculum spraying mechanism for spraying and inoculating liquid inoculum toward a container, wherein the lid of the cultivation container is opened / closed in a state of being stored in the container. In addition to opening and closing a predetermined number of cells, the liquid inoculum injection mechanism inoculates the liquid inoculum when opening the lid, so the inoculation work efficiency of the liquid inoculum can be increased and the opening time of the lid of the cultivation container is shortened as much as possible. Then, invasion of various bacteria can be suppressed. After the inoculation process is completed, the cultivation container housed in the container is transferred to the unloading side by the transfer mechanism. Further, at a predetermined operating position by the lifting device, each surface portion of a floor surface portion which is a surface portion of the culture medium in the cultivation container and an inoculation hole formed by being recessed in a part of the floor surface portion. In addition, it is possible to uniformly inoculate the liquid inoculum with the liquid inoculum injection mechanism. This makes it possible to favorably promote the growth of hyphae inside the culture medium by the inoculum.

According to an eleventh aspect of the present invention, in the sixth to eighth aspects, the cultivating container is formed by a cultivating bottle with a lid made of synthetic resin or the like, and a plurality of the cultivating bottles are vertically and horizontally aligned. A transport mechanism that supplies the stored container from the loading side and transports the container to the unloading side while intermittently transferring the cultivation bottle, and a lid of the cultivation bottle in the container that is intermittently transferred by the transportation mechanism in a horizontal row. A lid opening and closing mechanism that operates to open and close in units, and the liquid inoculum injection mechanism that supplies a predetermined amount of liquid inoculum to each of the cultivation bottles in a horizontal single row unit whose lid is opened by the lid opening and closing mechanism. A liquid seed culture inoculation device provided, which is intermittently transferred to the seed culture inoculation position by the transport mechanism, and the lid of the cultivation container in the container opens and closes in horizontal row units at the seed culture inoculation position. Opened by the closing mechanism, a predetermined amount of liquid inoculum is supplied to the cultivation container in the horizontal row unit whose lid is opened by the lid opening and closing mechanism, respectively, is inoculated by being supplied to the cultivation container through the liquid inoculum injection mechanism, After the inoculation, the lid is closed by the lid opening / closing mechanism, and the cultivation mechanism in the container is intermittently transferred to the unloading side in horizontal row units by the transport mechanism based on this repetition. At the time of this inoculation, the lid of the cultivation container in the container is opened in horizontal row units by the lid opening / closing mechanism, and at the time of opening, the liquid inoculum is inoculated by the liquid inoculum injection mechanism, so the inoculation work efficiency of the liquid inoculum can be improved. In addition, the opening time of the lid of the cultivation container can be shortened as much as possible, and invasion of various bacteria can be suppressed. Further, at a predetermined operating position by the lifting device, each surface portion of a floor surface portion which is a surface portion of the culture medium in the cultivation container and an inoculation hole formed by being recessed in a part of the floor surface portion. In addition, since it is possible to uniformly inoculate the liquid inoculum with the liquid inoculum injection mechanism, it is possible to favorably promote the growth of hyphae inside the culture medium by the inoculum.

[Brief description of drawings]

FIG. 1 shows a liquid seed germ filling bag obtained by filling liquid seed germs in small portions from a seed germ storage tank according to a first embodiment of the present invention, and an inoculation device using the liquid seed germ filling bag. It is an overall configuration schematic diagram.

FIG. 2 is an overall configuration diagram of a liquid seed germ-filled bag and an inoculation device showing a first embodiment of the present invention.

FIG. 3 is a front view showing a main part of the liquid seed culture inoculating device of FIG.

FIG. 4 is a front view of a main part showing an operating state of a lid opening / closing mechanism and a seed injection mechanism of the liquid seed inoculation device of FIG.

FIG. 5 is a front view of a main part showing a state in which the injection nozzle of the seed germ injection mechanism of FIG. 4 is moved downward toward the cultivation container side.

FIG. 6 is a front view of a main part showing a state in which liquid inoculum is ejected mainly from the floor surface position of the culture medium from an ejection nozzle after the inoculum ejection mechanism is moved toward the cultivation container side.

FIG. 7 is a front view of an essential part showing a state where the seed germ injection mechanism is further moved downward.

FIG. 8 is a front view of the main part showing a state in which the liquid inoculum is ejected mainly from the position of the inoculation hole of the culture medium from the ejection nozzle after further moving the inoculum ejection mechanism toward the cultivation container side. is there.

FIG. 9 is a plan view mainly showing a cultivation container positioning and holding mechanism that holds a shoulder portion of the cultivation container.

10 is a right side view showing a main part of the liquid inoculum injection mechanism corresponding to FIG. 3 when the lid is closed.

11 is a left side view showing a main part of the liquid inoculum injection mechanism when the lid is closed, corresponding to FIG.

FIG. 12 is a side view of essential parts showing a sprayed state of liquid inoculum by an injection nozzle of the liquid inoculum injection mechanism corresponding to FIG. 6.

13 is a side view of essential parts showing a sprayed state of liquid inoculum by an injection nozzle of the liquid inoculum injection mechanism corresponding to FIG. 8. FIG.

FIG. 14 is a side view showing a main part of a lid opening / closing mechanism corresponding to FIG. 3.

15 is a side view showing an operating state (lid holding state) of a movable holding member in the lid opening / closing mechanism of FIG. 14;

16 is a side view of essential parts showing a state where the lid of the cultivation container is removed from the state of the lid being clamped in FIG. 15.

FIG. 17 is a front cross-sectional view of an essential part showing a state in which the injection nozzle of the liquid seed germ injection mechanism corresponding to FIG. 5 is operated toward the cultivation container side.

FIG. 18 is a front cross-sectional view of a main part showing a sprayed state of liquid inoculum by an injection nozzle of the liquid inoculum injection mechanism corresponding to FIGS. 6 and 12.

19 is a front cross-sectional view of a main part showing a sprayed state of liquid inoculum by an injection nozzle of the liquid inoculum injection mechanism corresponding to FIGS. 8 and 13. FIG.

FIG. 20 is a schematic view of an inoculation process showing a state in which the liquid inoculum is sprayed mainly on the floor surface position of the culture medium with reference to the frontmost cultivation container.

FIG. 21 is a schematic view of an inoculation process showing a state in which liquid inoculum is sprayed mainly on the position of the inoculation hole of the culture medium with reference to the cultivation container in the front row.

FIG. 22 is a schematic diagram of an inoculation process showing a state where liquid inoculum is inoculated with the second row cultivation container as a reference.

FIG. 23 is a schematic diagram showing a container transportation state after completion of the inoculation step of all cultivation containers of the container.

FIG. 24 is a schematic view of a spraying process operation mainly showing an elevating device for elevating the liquid seed germ injection mechanism in the first embodiment.

FIG. 25 is a schematic view of a spraying step operation showing a state where the liquid seed germ injection mechanism in the first embodiment is moved to the mouth position of the cultivation container.

FIG. 26 is a schematic view of a spraying process operation showing a state where the liquid seed germ injection mechanism in the first embodiment is moved downward by the lifting device.

FIG. 27 is a schematic view of a spraying step operation showing a state where the liquid seed culture spraying mechanism in the first embodiment mainly sprays on the floor surface position of the culture medium.

FIG. 28 is a schematic diagram of a spraying process operation showing a state in which the liquid seed culture spraying mechanism in the first embodiment sprays mainly on the floor surface position of the culture medium and further moves downward.

FIG. 29 is a schematic view of a spraying step operation showing a state where the liquid seed culture spraying mechanism in the first embodiment mainly sprays at the position of the inoculation hole of the culture medium.

FIG. 30 is an overall configuration diagram showing a liquid inoculum-filled bag and an inoculation device according to the second embodiment of the present invention.

FIG. 31 is a schematic perspective view showing a part of a packaging container made of a rectangular packaging bag in a second embodiment in a cross section.

FIG. 32 is a schematic front view showing the main part of the liquid seed culture inoculation device according to the second embodiment.

FIG. 33 is a schematic side view showing the main part of the liquid inoculum inoculating device according to the second embodiment.

[Explanation of symbols]

B liquid inoculum C culture medium C1 floor C2 inoculum P Air supply source (compressor) R fixing screw S detection switch 1 inoculum storage tank 2 Liquid seed germ filling bag 3 inoculation device 4 inoculum supply pipeline 5 supply ports 6 Suction pipe 7 Pressurized container 7A container 8 Liquid inoculum pressurizing means (compressor) 8A Liquid inoculum pressurizing means (pressing plate) 9 Pressurized air hose 10 Pressure regulator 11 body frame 12 Transport mechanism 13 Operation panel 13A valve opening / closing drive control means 17 Lid 18 cultivation container (cultivation bottle) 18A cultivation container (packaging bag) 19 containers 20 Stopper mechanism 21 Cultivation container positioning and holding mechanism 22 Shoulder holding arm member 27 drive cylinder 29 Lid opening / closing mechanism 30 Liquid inoculum injection mechanism 31 Injection on-off valve 32 Gas pressurizing means (compressor) 33 filters 34 gas supply line 35 Solenoid valve 36 injection nozzles 37 Liquid seed germ injection cylinder body 41, 41A air cylinder 44 Fixed clamping member 45 Movable clamping member 46 Air cylinder 48 Transmission moving means (link arm member) 49 drive cylinder 50 rod 51 connecting rod 52 Lifting device 53,53A Nozzle position detection sensor 54, 54A solenoid valve 55,55A fluid conduit 56,56A fluid conduit 57, 57A fluid conduit

Claims (11)

[Claims] 1. A bag for filling a liquid seed culture having flexibility, which is obtained by subdividing and filling the liquid seed culture in an aseptic state from a seed storage tank in which the liquid seed culture is cultured and stored. 2. The liquid seed germ according to claim 1, wherein the liquid seed germ-filled bag is provided with a supply port that allows the liquid seed germ in the liquid seed germ-filled bag to be discharged to the outside. Filling bag. 3. The liquid seed germ-filled bag according to claim 1, wherein the liquid seed germ-filled bag is an inner bag, and the outer side of the bag is covered with an outer box made of a hard material. 4. A pouring step of supplying the liquid seed bacteria under pressure from the liquid seed bacteria-filled bag subdivided into an aseptic state through a seed bacteria supply pipe and sending the liquid seed bacteria to a liquid seed bacteria injection mechanism, and the liquid seed bacteria injection mechanism. A method for inoculating a liquid inoculum comprising a flow rate setting step in which a switching operation is performed so as to pressurize and spray a predetermined amount of the liquid inoculum onto the culture medium of the cultivation container from the injection nozzle provided in. 5. The method for inoculating a liquid inoculum according to claim 4, wherein the liquid inoculum is inoculated mainly on the floor surface position of the culture medium in the cultivation container during inoculation by an injection nozzle provided in the liquid inoculum injection mechanism. A method for inoculating a liquid inoculum, which comprises a step and a step of inoculating a liquid inoculum mainly on the position of an inoculation hole formed in the floor surface portion of the culture medium. 6. A flexible liquid inoculum in which the liquid inoculum is subdivided and filled in a sterilized state from an inoculum storage tank in which the liquid inoculum is cultured and stored in an inoculation device for inoculating the inoculum into a culture medium in a cultivation container. Filling bag, inoculum supply line for supplying liquid inoculum from the liquid inoculum filling bag, and liquid inoculum for pressurizing and delivering the liquid inoculum along the transport route of the liquid inoculum filling bag or the inoculum supply line Pressure supplying means, a liquid inoculum injection mechanism for spraying and inoculating the liquid inoculum pressurized and supplied from the liquid inoculum pressure supplying means to the culture medium of the cultivation container, and the liquid inoculum injection mechanism provided in the liquid inoculum injection mechanism, A liquid inoculum which is provided with a spray on-off valve for supplying and shutting off the liquid inoculum pumped from the liquid inoculum filling bag via the inoculum supply line. Inoculation device. 7. The inoculation of the liquid inoculum according to claim 6, further comprising valve opening / closing drive control means for switching the spray on / off valve to set a flow rate or inoculation time for inoculating the liquid inoculum. apparatus. 8. The liquid inoculum injection mechanism is provided with an elevating mechanism capable of moving toward and away from the cultivation container, and the liquid inoculum injection mechanism performs the cultivation at a predetermined operating position by the elevating mechanism. While inoculating by spraying liquid inoculum mainly on the floor position of the culture medium in the container,
8. The liquid inoculum according to claim 6 or 7, wherein the liquid inoculum is configured to be sprayed and inoculated mainly with the position of the inoculation hole formed in the floor portion of the culture medium. Inoculation device. 9. The liquid inoculum inoculation device according to claim 6, wherein the cultivation container is formed of a packaging bag made of synthetic resin or the like. 10. A cultivation bottle with a lid made of synthetic resin or the like is formed as the cultivation container, and a container accommodating a plurality of the cultivation bottles aligned vertically and horizontally is supplied from the loading side, and the cultivation bottle is A transport mechanism for transporting the container to the unloading side while carrying out intermittent transport, and a lid opening / closing mechanism for sequentially opening and closing a predetermined number of lids of the cultivation bottles in the container transported to the seed inoculation position by this transport mechanism, The liquid inoculum injection is provided in accordance with the number of cultivation bottles opened and closed by the lid opening and closing mechanism, and the liquid inoculum is sprayed and inoculated toward the culture medium in the cultivation bottle whose lid is opened by the lid opening and closing mechanism. 9. The liquid inoculum inoculating device according to claim 6, further comprising a mechanism. 11. A cultivation bottle with a lid made of synthetic resin or the like is formed as the cultivation container, and a container accommodating a plurality of the cultivation bottles aligned vertically and horizontally is supplied from the loading side, and the cultivation bottle is A transport mechanism that transports the container to the unloading side while performing intermittent transport, a lid opening and closing mechanism that opens and closes the lid of the cultivation bottle in the container intermittently transported by this transport mechanism in horizontal row units, and this lid opening and closing mechanism The liquid inoculum injection mechanism for supplying a predetermined amount of the liquid inoculum into each of the cultivation bottles in a horizontal row unit with the lid open, and the liquid inoculum injection mechanism. The liquid inoculum inoculation device according to.