JP2003146803A - Antimicrobial powder composition and method for producing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a new antimicrobial powder composition produced by a method having excellent aspects of production efficiency and the method for producing the composition. SOLUTION: This antimicrobial powder composition comprises a cyclodexterin inclusion substance of a volatile oily antimicrobial substance and a water absorbing inorganic solid substance. The method for producing the composition comprises mixing and stirring the volatile oily antimicrobial substance with cyclodextrin in water, producing the cyclodexterin inclusion substance of the volatile oily antimicrobial substance and then charging the water absorbing inorganic solid substance having water absorbing actions into the system wherein the cyclodextrin inclusion substance is present and thereby absorbing water present in the system into the water absorbing inorganic solid substance. The resultant mixture, if necessary, is pulverized and powdered.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a novel antibacterial powder composition produced by a method excellent in production efficiency and a method for producing the same.

[0002]

2. Description of the Related Art Volatile oily antibacterial substances such as isothiocyanic acid esters represented by allyl isothiocyanate are known as substances for preventing the formation of mold and bacterial growth in foods. However, since the substance is highly volatile, it has a characteristic that the effect is reduced in an extremely short time, and thus there is a problem in terms of sustainability of the effect itself. Therefore, as one means for solving such a problem, a method is used in which a volatile oily antibacterial substance is included in cyclodextrin so that the substance can be gradually released, and then used. In addition, inclusion of a volatile oily antibacterial substance in cyclodextrin is expected to have effects such as chemical stabilization, deodorization, oxidation prevention, and photodegradation suppression of the substance. Therefore, by including the volatile oily antibacterial substance in the cyclodextrin, the long-term effectiveness and stability of the substance can be ensured.

[0003] Usually, a method of encapsulating a volatile oily antibacterial substance in cyclodextrin is carried out by mixing and stirring cyclodextrin and the volatile oily antibacterial substance in water. The cyclodextrin clathrate of the volatile oily antibacterial substance produced in this manner is vaporized to remove water present in the system by spray drying or drum drying to obtain an antibacterial powder composition.

[0004]

However, the spray drying and the drum drying as described above are performed at a high temperature. Therefore, even though the volatile oily antibacterial substance is included in the cyclodextrin, the volatile oily antibacterial substance volatilizes to some extent in the process, and thus such a method is not satisfactory in terms of production efficiency.
Therefore, an object of the present invention is to provide a novel antibacterial powder composition produced by a method excellent in production efficiency and a method for producing the same.

[0005]

The antibacterial powder composition of the present invention made in view of the above points is as described in claim 1.
It is characterized by comprising a cyclodextrin clathrate of a volatile oily antibacterial substance and a water-absorbing inorganic solid substance. The antibacterial powder composition according to claim 2 is characterized in that, in the antibacterial powder composition according to claim 1, the water-absorbing inorganic solid substance is a substance capable of retaining crystal water. The antibacterial powder composition according to claim 3 is the antibacterial powder composition according to claim 1 or 2, wherein the water-absorbing inorganic solid substance is a water-soluble substance. The antibacterial powder composition according to claim 4 is the antibacterial powder composition according to any one of claims 1 to 3, wherein the water-absorbing inorganic solid substance is at least selected from alum, sodium sulfate and magnesium sulfate. It is characterized in that it is one. The antibacterial powder composition according to claim 5 is the antibacterial powder composition according to any one of claims 1 to 4, wherein the volatile oily antibacterial substance is an isothiocyanate ester. Further, according to the method for producing an antibacterial powder composition of the present invention, as described in claim 6, the volatile oily antibacterial substance and the cyclodextrin are mixed and stirred in water to form a cyclodextrin clathrate of the volatile oily antibacterial substance. After soaking, by adding a water-absorbing inorganic solid substance having a water-absorbing action into the system in which the cyclodextrin clathrate is present, the water-absorbing inorganic solid substance is allowed to absorb water present in the system, and thus obtained It is characterized in that the mixture is pulverized and powdered if necessary.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION The antibacterial powder composition of the present invention is characterized by comprising a cyclodextrin clathrate of a volatile oily antibacterial substance and a water-absorbing inorganic solid substance,
A volatile oily antibacterial substance and cyclodextrin are mixed and stirred in water to form a cyclodextrin clathrate of the volatile oily antibacterial substance, and then a water absorbing property having a water absorbing action in the system in which the cyclodextrin clathrate is present. By adding an inorganic solid substance, the water-absorbing inorganic solid substance is made to absorb water existing in the system, and the resulting mixture is pulverized and powdered if necessary. That is, the antibacterial powder composition of the present invention has hitherto been obtained by spray drying or drum drying in order to obtain a cyclodextrin clathrate of a volatile oily antibacterial substance, and then to obtain the antibacterial powder composition. Instead of volatilizing and removing water present in the system, a water-absorbing inorganic solid substance having a water-absorbing action is added to the system in which the cyclodextrin clathrate is present, and is present in the system. An antibacterial powder composition is obtained by absorbing water. Therefore, since the antibacterial powder composition of the present invention is not exposed to high temperature in the manufacturing process, the volatile oily antibacterial substance is not volatilized during the manufacturing process and can be manufactured with excellent efficiency. It is something.

The volatile oily antibacterial substance applied as the antibacterial powder composition of the present invention means an oily substance which is volatile at room temperature and exerts an antibacterial effect. For example, a pungent component of mustard or wasabi. The isothiocyanic acid ester and the monoterpene compound, which is abundantly contained in natural plants, correspond to this. These are natural products obtained from plant extracts and the like, and are highly safe for the human body. In particular, isothiocyanates are suitable for food hygiene because they exhibit an excellent antibacterial action. The volatile oily antibacterial substance is not limited to a natural product, and may be a synthetic product synthesized by a method known per se.

Specific examples of isothiocyanates include allyl isothiocyanate, phenyl isothiocyanate, methyl isothiocyanate, ethyl isothiocyanate, propyl isothiocyanate, isopropyl isothiocyanate, butyl isothiocyanate, isobutyl isothiocyanate, isoamyl isothiocyanate and isothiocyanate. Examples thereof include benzyl and cyclohexyl isothiocyanate.

Examples of the monoterpene compound include terpene hydrocarbon, terpene alcohol, terpene aldehyde, terpene ketone and terpene oxide.

As the cyclodextrin for encapsulating a volatile oily antibacterial substance, in addition to α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin, branching or modification of maltosyl cyclodextrin, dimethyl cyclodextrin, trimethyl cyclodextrin, etc. Cyclodextrin, cyclodextrin polymer, etc. are used. These cyclodextrins are highly safe in terms of food hygiene and have the property of stabilizing and effectively exhibiting the antibacterial action of volatile oily antibacterial substances. Above all,
Α-Cyclodextrin and β-cyclodextrin are preferably used from the viewpoints of inclusion property with respect to volatile oily antibacterial substances and economy.

The method of encapsulating the volatile oily antibacterial substance in cyclodextrin is not particularly limited, and for example, 0.5 part of water is added to 1 part by weight of cyclodextrin.
Add 5 parts by weight of volatile oily antibacterial substance to 0.01
~ 0.15 parts by weight is added, and a kneader or the like is used for 20 ~ 4.
The method of stirring at 0 degreeC for 1-2 hours is mentioned.

The cyclodextrin clathrate, which is a volatile oily antibacterial substance produced by the above-mentioned method, has a low water solubility and thus precipitates as a precipitate. As described above, according to the conventional method, water existing in the system is volatilized and removed by spray drying or drum drying to obtain an antibacterial powder composition. However, in the method of the present invention, such an operation is not performed, and a water-absorbing inorganic solid substance having a water-absorbing action is introduced into the system to absorb water, and inclusion of cyclodextrin of a volatile oily antibacterial substance is carried out. A solid product consisting of the body and the absorbed inorganic solid substance is obtained. This is crushed and powdered as needed to obtain an antibacterial powder composition.

As the water-absorbing inorganic solid substance to be introduced into the system in which the produced cyclodextrin clathrate of the volatile oily antibacterial substance is present, a substance capable of retaining absorbed water as crystal water is preferably used. . Water-absorbing inorganic solid substance that retains all water present in the system as crystal water (To retain all water used to form cyclodextrin inclusion complex of volatile oily antibacterial substance as crystal water) The required solid amount can be calculated from the number of moles of both), and the solid substance obtained is not a moist product but is dry. The antibacterial powder composition can be easily obtained without the drying step. In addition, in the system, in addition to the reaction system in which a volatile oily antibacterial substance is included in cyclodextrin to form a cyclodextrin clathrate, the cyclodextrin clathrate is separately separated from the reaction system by an appropriate method. It also means the inside of the system where water is collected but remains.

Specific examples of the water-absorbing inorganic solid substance capable of retaining water of crystallization include alum (capable of retaining 12 crystal water), sodium sulfate (capable of retaining 10 crystal water), calcium sulfate (capable of retaining 10 crystal water). ), Magnesium sulfate (capable of holding 10 water of crystallization) and the like. Since these are recognized as food additives, they are convenient in that they can be applied to various applications including foods. In particular, alum has been used as a color-preserving agent for pickles and simmered foods for a long time, so that alum is used as a water-absorbing inorganic solid substance when preventing the generation of mold or bacterial growth on pickled or simmered foods. With the antibacterial powder composition, alum also functions as a color retention agent.

The water-absorbing inorganic solid substance capable of retaining water of crystallization may be a water-soluble substance or an insoluble substance, but the antibacterial powder composition of the present invention is applied to an aqueous solution. In this case, when the antibacterial powder composition using a water-soluble water-absorbing inorganic solid substance is added to the application target, the substance dissolves in the aqueous solution, so that the appearance (appearance) of the application target is not impaired. Examples of such water-soluble water-absorbing inorganic solid substances include alum, sodium sulfate, magnesium sulfate and the like among the above-mentioned water-absorbing inorganic solid substances capable of retaining water of crystallization.

After the water-absorbing inorganic solid substance is put into the system in which the cyclodextrin clathrate of the volatile oily antibacterial substance is present, the water present in the system is absorbed by, for example, leaving it at room temperature overnight. Absorbed by a solid inorganic solid material, sometimes a lumpy solid remains in the system. The antibacterial powder composition of the present invention can be obtained by pulverizing and pulverizing this if necessary.

The antibacterial powder composition of the present invention obtained as described above ensures the effectiveness and safety of the volatile oily antibacterial substance for a long period of time, so that it is possible to prevent mold and bacterial growth. It can be applied to various application objects that need to be prevented. For example, when the antibacterial powder composition of the present invention is applied in the process for producing pickles, the method of use is to add the composition to salt water for salting or various seasonings, and to use the composition. There are a method of spraying a solution prepared by adding it to water onto vegetables, and a method of washing vegetables with this solution. When using allyl isothiocyanate as a volatile oily antibacterial substance, the concentration of allyl isothiocyanate in the solution exerts sufficient antibacterial properties against Escherichia coli, etc., and the pickles are excessively irritating odor and pungency peculiar to allyl isothiocyanate. It is desirable to adjust the concentration to 1 ppm to 1000 ppm before use in order to prevent the adherence of such substances. When the antibacterial powder composition of the present invention is used by adding it to a seasoning, since the seasoning is consumed by consumers together with pickles, the allyl isothiocyanate concentration in the seasoning can be as high as possible. It is desirable to make it low, and it is good to set it to 150 ppm or less.

For example, in the process of salting vegetables,
In the case of the mode in which the antibacterial powder composition is added to the salt water, even if the amount of salt added is reduced, the growth of microorganisms can be effectively suppressed by the action of the antibacterial powder composition, so sufficient storage stability is ensured. can do. Therefore, in the desalting step, desalting can be easily performed without requiring a large-scale device and a large amount of water.

The pickles to which the antibacterial powder composition of the present invention using allyl isothiocyanate as a volatile oily antibacterial substance is added are ready-made foods that are eaten as a side food as they are, Chinese cabbage, Nozawana, spinach, onion, bean sprouts, butterbur, radish, garlic,
Lotus root, radish, turnip, carrot, burdock, potato, sweet potato, pumpkin, cucumber, eggplant, cucumber, ume, various fruits, mushrooms and seaweed (hereinafter referred to as vegetables) as main ingredients, salt, soy sauce, miso Soup, dregs, koji, vinegar, bran (rice bran, bran, etc.), mustard, moromi, and other seasonings. There are some pickles that are aged after pickling and then preserved with salt, alcohol, acid, etc., or those that are poorly preserved, such as light pickles such as overnight pickles. According to the seasonings used, pickles are salted in salt-based seasonings (Lakkyo salt pickles, tsubo pickles, plum pickles, Nozawana pickles, etc.), and soy sauce-dried seasonings. Soy sauce pickles (Fukujin pickles, Takana pickles, Chosen pickles, etc.), miso pickles that are miso-pickled in seasonings (Yamasai miso pickles, radish miso pickles, etc.), Kasuke pickled in spices (Nara) Pickles, wasabi pickles, etc.), Koji pickles pickled in seasonings mainly made of koji (Betara pickles, Sangoha pickles, etc.), vinegar, plum vinegar or seasonings mainly made of organic acids, pH 4.0 or less. Pickled vegetables (Senmai-zuke, Rakkyo-zuke, Hari-harizuke, etc.),
Nuka-zuke (Takuan-zuke, etc.) that has been soaked in seasoning mainly made of rice bran, Soy-zuke (soup with eggplant and fuki-karashizuke) with soybean flour, etc., soy sauce or miso moromi In addition to moromi-zuke (Konasu moromi-zuke, cucumber moromi-zuke, etc.), which is mainly seasoned with seasonings, it is also classified into other pickles (including fermented pickles) such as immediate pickles and sauerkraut.

[0020]

EXAMPLES The antibacterial powder composition of the present invention will be described in more detail by way of examples, but the present invention is not limited to the following description.

Formulation Example 1: Production of antibacterial powder composition Part 1 1.1 g of allyl isothiocyanate was added to a suspension prepared by adding 8 g of water to 10 g of α-cyclodextrin, and using a stirrer in a beaker. And stirred at room temperature for 2 hours. Next, 8 g of water was absorbed as crystallized water, and the resulting alum (dry aluminum potassium sulfate) was added to a 12 g beaker, stirred and left overnight, and the contents in the beaker were dried. It became a lumpy white solid. The lumpy white solid was taken out of the beaker and crushed into a powder to obtain a dry antibacterial powder composition containing an allyl isothiocyanate α-cyclodextrin inclusion complex.

Formulation Example 2: Production of antibacterial powder composition No. 2 In the same manner as in Formulation Example 1, 4 g of β-cyclodextrin,
From 16 g of water, 0.4 g of allyl isothiocyanate and 20 g of baked alum, a dry antibacterial powder composition containing a β-cyclodextrin clathrate of allyl isothiocyanate was obtained.

Experimental Example 1: Additive effect of antibacterial powder composition in the process of salting vegetables (cucumber salting) 5% by volume salted water as salting solution of cucumber, 10% by volume
Four kinds of salt water, 15% by volume salt water and 20% by volume salt water were prepared. Of these, 2% by volume of the antibacterial powder composition obtained in Formulation Example 1 was added to and dissolved in 3 types of salt water other than 20% by volume (the concentration of allyl isothiocyanate in the pickling solution was 500 ppm). Next, add half of the pickled cucumber to the well-washed cucumber,
In this state, it was stored at room temperature, and the fluctuation of the number of general viable bacteria in the pickling solution from the start of storage was examined. The results are shown in Table 1.

[0024]

[Table 1]

As is clear from Table 1, in the control example, the number of bacteria decreased once after the start of storage, but after that, there was almost no change in the number of bacteria and a relatively high number of bacteria was maintained. In Test Example 1, the number of bacteria was smaller than that of the control example 7 days after the start of storage, but after that, the number of bacteria was almost the same as that of the control example. In Test Example 2 and Test Example 3, 5 from the start of storage.
Even after 6 days, there was no tendency for the number of bacteria to increase, and the number of bacteria was kept low as compared with the control example. From the above results, by using the salt and the antibacterial powder composition of the present invention together, even if the salt concentration is about half of 20% by volume, which is the salt concentration generally used so far, it is sufficient. It was found that a good preservation property can be secured. Therefore, it has been found that it is possible to reduce the amount of water used and the process time in the desalting process during secondary processing, and it is possible to achieve cost reduction.

[0026]

INDUSTRIAL APPLICABILITY The antibacterial powder composition of the present invention is not exposed to high temperature in the manufacturing process thereof, so that the volatile oily antibacterial substance is not volatilized during the manufacturing process and the excellent efficiency is obtained. It is manufactured by.

   ─────────────────────────────────────────────────── ─── Continued front page    F-term (reference) 4B021 LW02 MC01 MK02 MK24 MP02                 4B069 DA03 DA14 KA01 KB03 KC11                       KC21                 4H011 AA01 BA01 BA04 BB11 BC18                       BC19 DA02 DF03 DH10

Claims (6)

[Claims] 1. An antibacterial powder composition comprising a cyclodextrin clathrate of a volatile oily antibacterial substance and a water-absorbing inorganic solid substance. 2. The antibacterial powder composition according to claim 1, wherein the water-absorbing inorganic solid substance is a substance capable of retaining water of crystallization. 3. The antibacterial powder composition according to claim 1, wherein the water-absorbing inorganic solid substance is a water-soluble substance. 4. The water-absorbing inorganic solid substance is alum,
The antibacterial powder composition according to any one of claims 1 to 3, which is at least one selected from sodium sulfate and magnesium sulfate. 5. The antibacterial powder composition according to any one of claims 1 to 4, wherein the volatile oily antibacterial substance is an isothiocyanate ester. 6. A volatile oily antibacterial substance and cyclodextrin are mixed and stirred in water to form a cyclodextrin clathrate of the volatile oily antibacterial substance, and then water is absorbed into the system in which the cyclodextrin clathrate is present. By adding a water-absorbing inorganic solid substance having an action, the water-absorbing inorganic solid substance is made to absorb the water present in the system, and the resulting mixture is pulverized and powdered if necessary. A method for producing an antibacterial powder composition.