JP2003139769A - Device for automatically determining chemical - Google Patents

Abstract

PROBLEM TO BE SOLVED: To automate inspection and determination, to avoid a bias in visual determination, to input color reaction inspection by a CCD image, and to display and record a determined result through image processing. SOLUTION: This device comprises an input part for inputting information consisting of mainly character information as in an attribute of a subject and environment information, for example, by a memory card, a setting part (reagent reacting part) 20 for a specimen pad, a lighting system 30 for lighting the specimen pad, an image inputting part 32 for image-inputting the specimen pad by a CCD camera, an image display part 33, an image converting part 34, an image processing part 35, a record display part 36, a determination outputting part 37, an integral control part 38 and a device including an electric power source 40 related to all of them. The presence of chemicals in the specimen (urine) is automatically judge-determined by color reaction of a reagent to display a result therein, a recorded data thereof is record-stored to be stored over a long period.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a simple determination device for assaying the presence or absence of a drug or a modified substance derived from the drug present in blood, saliva, urine or the like (hereinafter referred to as a sample) when a drug is ingested, and In detail, by injecting / injecting a target sample into a sample pad containing a single or a plurality of reagents, a reagent reaction is caused between the sample and the reagent, and preferably a reagent that causes coloration / discoloration associated with the reagent reaction Select the reaction, judge the presence or absence of the drug or the modified substance derived from the drug from the test result based on the color reaction, and record the fact including the test result in the subject (specimen donor) and save it. The present invention relates to an automatic drug determination recording device.

[0002]

2. Description of the Related Art Various means for testing the presence or absence of a drug in a specimen are known. Although the subject of inspection is different from that of the present invention, as a common example, the determination of the presence of sugar in urine is established by a simple test paper. Depending on the sugar concentration, the color changes from dark green to pale yellow, and it is unlikely that the judgment will be erroneous due to the vivid coloration, color tone, and difference in shade.
On the other hand, although it is not easy to determine whether the driver who is a while after drinking alcohol is still drunk, it is necessary to qualitatively or quantitatively test the presence and amount of alcohol from breath and blood. Is possible.

In the present invention, a stimulant or the like is selected as a target drug, and urine, for example, is used as a sample.
Conventionally, in this field, there have been known an analytical test method using a chromatograph and a simple test pad method for easily carrying out an inspection. The former is a full-scale test method, but it requires labor and time, and also requires a certain degree of skill (operation technique). Therefore, the simple latter is useful and practical in the field, and is expected to play a primary test role.

[0004]

However, there is a problem that the simple test pad method cannot store the test results for a long period of time. To explain this test method in detail, in the assay pad in which the reagent is set, the sample (urine) is placed in the column (sample injection hole) of the drug to be assayed in the pad.
Inject and drop a few drops. After a few minutes to 10 minutes until the reaction is completed, visually check the colored display part of the colored (discolored) assay pad, and also visually check the presence or absence of a change in color tone in the prescribed drug column. The test state is concluded by discriminating the test state of the urine reagent as "positive, false positive or negative".

Although this operation is simple and the reaction with the reagent is simple, the judgment method is visual, so that there are inherent differences among the inspectors, and the objectivity of the inspection results is somewhat problematic. In addition, the coloring reaction of the assay pad fades or discolors over time and cannot be stored for a long time.
Therefore, there is a problem that the requirement for official evidence data cannot be established.

[0006]

DISCLOSURE OF THE INVENTION The present invention has been improved in order to solve this problem, and is a technique of utilizing a color reaction between a specimen and a reagent. Then, revising that the examiner visually carries out the coloring state of the prior art,
Objectively detecting the coloring state under appropriate illumination using CCD which is an electronic camera, and CC on behalf of the examiner
The image input data of D is processed by a computer so that no artificial data is added to the determination result. Also,
Since a computer is used, not only the test results can be displayed but also recorded, so the fact of the coloring reaction can be proved, the problems of fading and discoloration can be solved, and the requirements for official evidence data are established. You can

Specifically, the screen of the assay pad during and after the reaction of the reagent is captured as an image inside the computer by the CCD camera, and image processing and image determination are performed by a predetermined algorithm (data processing program). It is an automatic judgment recording means for displaying a result and automatically recording the result, especially for a field.

It is possible to combine this function into a simple device. The exterior of the device is equipped with a sample pad mounting part similar to the conventionally used assay pad, an input part for environmental information including the subject, a liquid crystal screen for displaying judgment results, etc. An illumination for the sample pad, a CCD camera, a computer arithmetic function including image processing, and the like are stored. The entire device is about the size of a telephone, making it convenient to carry.

[0009]

In order to achieve the above-mentioned object, the device invention according to claim 1 is an information input section mainly for character information such as attribute / environment information of a subject, Sample pad set part (sample pad insertion and reagent reaction part) into which human urine can be injected, illumination system consisting of artificial illumination including RBG three-color components, CCD image input part sensitive to the illumination system, image processing part, RGB Image decomposition unit, determination algorithm processing unit, determination output unit, the character information, the image information,
It includes a storage unit for determination records and the like, and a reagent reaction determination recording unit capable of automatically determining and recording a drug test including an integrated control unit.

If a memory card or the like is used as the input unit for the information mainly consisting of character information, it is simple, but the keyboard can be provided without any problem. It is preferable that the sample pad can be easily attached and that a protective means can be provided to prevent the sample and the reagent from leaking and contaminating the device. It is preferable that the illumination system is provided with a light emitting means capable of appropriately adjusting the balance of the RBG three-color components.

The CCD image input unit, the image processing unit, the RGB image decomposition unit, the determination algorithm processing unit, the determination output unit, the storage unit for the character information, the image information, the determination record, and the integrated control unit are commercially available personal computers. There is no difference from the function of the computer. For example, the CCD camera has about 400000 to 4000000 pixels, the central processing function (CPU) of the computer is about 200 to 1000 MHz, and the OS to be used is used by the general public such as Windows (registered trademark). You can do it.

In order to achieve the above-mentioned object, the apparatus invention according to claim 2 specifies illumination, which is an important requirement that may affect the determination result. Here, an illumination means including an RBG three-color component is provided with an illumination system capable of automatically detecting the optimum illumination wavelength and automatically adjusting to the optimum illumination wavelength. The coloration caused by the reaction between the sample and the reagent is related to the wavelength of the illumination, and if the wavelength of the illumination is selected so as to have a complementary color relationship with the color tone of the color reaction, the sensitivity of the CCD becomes maximum. Nor. So first,
It is possible to detect the coloration state of the specimen at the time of reaction, adjust the color balance for each brightness of RGB in the illumination, and input the coloring state as an image with the CCD camera under the optimal illumination. These operations can be carried out quickly in seconds, and there is no problem in comparison with minutes or hours, which is the development of general coloring reaction. It should be noted that even if a standard white wavelength is fixedly used for the illumination system, it can be used as a suboptimal means, and an error (deviation) rarely causes a mistake in the color reaction.

In the invention according to claim 3, the actual operation is CC
When a color change or discoloration occurs due to the reaction between a certain substance in urine as a sample and a reagent,
At least two color tones are automatically detected in the sample pad, a site where coloration or discoloration occurs (inside the reaction area) and a site where the sample and the reagent do not sufficiently react (peripheral site). This operation scans changes in the coloring state to determine the position to be input, detects deviation data from the coloring degree,
It is equipped with a program for comparison calculation. An image processing algorithm processing means is provided.

In the invention according to claim 4, the actual operation is CC
This is carried out by a D camera, and at least two positions of blank data (control) before sample injection and a site (inside the reaction area) where coloration or discoloration should occur after sample injection are detected, and these are compared and calculated. However, it is provided with an image determination means for determining a significant difference. The data of claim 3 and the data of claim 4 complement each other to contribute to secure the determination result.

In the invention according to claim 5, a time series change model in which standard color tone change until the reaction of the sample and the reagent is completed is prepared as data, that is, the time series model is set in the computer. Is recorded, and when the actual inspection starts,
An image determination means is provided for comparing and calculating the coloration or discoloration in the reaction area after the sample injection and the time-series change model to determine a significant difference. By predicting the coloration or discoloration in this way, it is possible to deal with an abnormal situation, avoid the occurrence of an operation error, and eliminate the data when an operation error occurs. The data of Example 1 is this model.

According to a sixth aspect of the present invention, the color tone of the illuminating means is adjusted based on the color tone (wavelength) of the site (inside the reaction area) where coloration or discoloration has occurred in the sample pad due to the reaction between the sample and the reagent. Is. As described above, it is optimal that the color tone of the illumination and the hue of the coloration reaction have a complementary color relationship with each other. In order to adjust the light emitting means, each light component RGB in the illumination is adjusted independently or the interrelationship is taken into consideration, based on the detection result of the three-color RBG component, the grayscale model and the infrared region indicated by the image data. And can be optimized. Furthermore, it is also possible to optimize the illumination state by performing an analytical calculation by color separation in the color image. Therefore, in the device of the present invention, it is possible to implement the former or the latter optimization means of the illumination state.

According to the seventh aspect of the present invention, it is intended to select a data processing program by paying attention to the point that the coloration state is different depending on the concentration of the specific substance occupied in the sample by the reaction between the sample and the reagent. Is. That is, in the test algorithm, there are two methods of making a judgment based on a population comparison (a magnitude relationship of variance values) of luminance distributions depending on the coloration state or a simple average of individual data for comparison judgment. When the specific substance has a high concentration, the image obtained by the CCD camera has high brightness, so the former method is suitable. When the concentration is relatively low, the calibration curve (concentration of the specific substance) and the brightness (coloration) are used. From the relationship of, the latter can be judged by a simple average value. Here, the content of the verification algorithm is to incorporate two programs so that the computer can select it depending on the situation and select either the former program or the latter program.

The invention according to claim 8 is provided with a position control mechanism for ensuring high accuracy by a multi-pole method when inserting the sample pad into the optical system inside the apparatus. This multi-pole system is a positioning means configured to be arranged at the same position each time the sample pad is inserted by using a precise roll and magnet.

[0019]

DETAILED DESCRIPTION OF THE INVENTION The present invention will be described with reference to the drawings.

FIG. 1 is a perspective view of a main body 10 of an automatic drug test determination device according to the present invention. When a memory card is inserted into a memory card insertion slot 11 of this device, information regarding a person who provides a sample, and information at the time of the test are shown. Information about environment etc. is entered. Further, the actual sample is dropped / injected into the hole provided in a predetermined column of the sample pad 20. Then, the sample pad containing the sample is inserted into the apparatus main body from the sample pad insertion port 12 and is accurately attached to a predetermined position of the main body apparatus. Then, the main unit automatically starts the inspection, and the determination result is displayed on the display unit 14 on the right side of the main unit in the form of "positive", "suspicious positive" or "negative" based on the determination logic of predetermined programming. To do. If necessary, the built-in printer can print out the determination result. Further, the display unit 13 on the left side displays information such as the name, date of birth, address, etc. of the subject, inspection date, and inspection place. Of course, if necessary, this information is printed together with the determination result.

The means for accurately determining the position is as described in claim 8. Of course, conventional positioning means can be applied as they are.

FIG. 2 shows a front view of the sample pad. This sample pad has a hole (or a slit) formed in the surface of the column on which the reagent is fixed, and is configured so that a sample such as urine can be injected into this hole. The sample is provided with slits in two rows or three rows, and the color reaction and the setting position of the reagent are designated. Then, it is desirable to quickly insert the sample pad into which the sample has been injected into the predetermined insertion port 12 of the main body device.

Next, an example in which a specific substance (stimulant or denatured substance derived from the stimulant) contained in urine (sample) of a subject who has taken the stimulant is tested by an assay pad will be described.

Since there are various stimulants, colored bars are shown for each component parameter according to these test target components, and in the case of a so-called normal state where the specific substance component is not contained in the sample, the color of the reagent is Appears as it is. For example, methamphetamine appears as a red colored bar, and when a specific substance component is contained, it reacts and fades according to its concentration, and becomes whiter / transparent as the concentration increases. However, depending on the type and type of the sample pad, the opposite is true. In the normal state (when no specific substance is present), it is white and transparent, and when the specific substance is present, it becomes colored, and the higher the concentration, the higher the degree of coloring.

The components and parameters to be examined are arranged in groups of 2 to 3 rows as shown in FIG. 2, and a depression (hole) for dropping a sample (urine in this case) is provided at the bottom of each row. At the top of each row, there is a control section that indicates that the color reaction of that row has reached the steady-state zone.

At the time of inspection, the bottom hole of each row is
When 3 to 4 drops of urine are dropped, the coloring reaction in each row begins,
The coloring zone rises with the passage of time, and the degree of color reaction proceeds. That is, the light red zone once advances in the row above the sample pad, and is colored (normal, no component) or whitened (containing component) in a predetermined test component zone.

The judgment of coloration and the elapsed time that can be inspected are confirmed by confirming the red coloring of the uppermost "control section". The time required for the determination is about 3 to 10 minutes. In the case of the above-mentioned coloring, the degree of coloring of each component and the result of coloring each component are used for the judgment.

FIG. 3 is a block diagram showing the entire system of the device of the present invention.

The automatic drug test determination apparatus according to the present invention includes an input section 11 for inputting mainly character information such as a subject attribute and environmental information with a memory card, a sample pad setting section (reagent reaction section) for setting a sample pad. ) 20, an illumination system 30 for illuminating a sample pad, etc., an image input section 32 for capturing the sample pad with a CCD camera and inputting it as CCD optical information (image). The image of the sample pad can be captured.), The image display section 33 for visually confirming the captured image, the color image in red “R”, green “G”, blue “B” and gray “gray scale”. An image conversion unit 34 for converting (this image conversion can also be converted to a predetermined wavelength input image), and the converted image is converted by a predetermined program. "Positive", "false positives", "negative" by calculation and the image processing unit 35 which processes the comparisons, (image processing
Is determined. This will be described in detail in step 6 below. ), A record display unit 36 for displaying the determination result on the front surface of the main body device, a display lamp for display output, a voice output, a determination output unit 37 capable of using print data, and control of each unit for automatic measurement. The integrated control unit 38 and the power supply unit 40 relating to all of them can store the data for automatically determining and recording the drug test. The power supply unit is designed to be compatible with AC, DC, automobile power supply, etc. so that the device of the present invention can be carried and used in the field.

Although the present invention relates to a device, FIG.
The explanation will be given in line with the system of FIG. 1 as the chart and the step procedure showing the flow as they are. Step 1: Insert a memory card into the main unit and input information. Alternatively, information may be input using a keyboard. Step 2: Specimen in a predetermined slit or hole on the specimen pad
(Urine) is dropped. The reaction with the reagents begins. Step 3: Attach the sample pad to the main body device. At this time, the positioning is completely performed. Step 4: Select the wavelength of the illumination system from preliminary observation of the color reaction of the sample pad and color tone analysis of the image. Step 5: Determine the wavelength of the illumination system and CC under that illumination
Input D image. Step 6: Image processing, especially data processing according to claims 3 to 5 is executed. Step 7: Comparing the data that have undergone the calculation, a judgment of "positive, false positive, negative" is made. Step 8: Display the judgment result. Step 9: The judgment result and the information of Step 1 are recorded and stored / stored.

Of the above, the image processing of step 6 will be supplemented.

After step 3, the coloring (discoloring / fading) reaction of the sample with the reagent has started. Therefore, a white LED is used for illumination, or a green system illumination is used because a normal sample exhibits a red color. The sample pad set in the main body device has a color change or discoloration part (inside the reaction area) and a part (peripheral part) where the sample and reagent do not sufficiently react (that is, almost the entire surface). The red "R" component is scanned and then the blue "B" component is similarly scanned. In this way, the color tone data of the surrounding reference zone and the colored reaction zone to be inspected are acquired, and the coloring data and the surrounding reference data are calculated from the maximum (inside R / coloring) and minimum (inside B / periphery) values. And the difference (coloring deviation) between them.

The specimen pad of FIG. 2 has "C" (control), "MET" (methamphetamine), "A" on the surface.
It is printed with "MP" (methamphetamine).
A frame area is defined as a range in which the CCD camera scans the determination data acquisition zone. These are for standardizing and standardizing the measurement conditions, and are designed to ensure the reproducibility of the measured values for the same sample so that the test can be performed under the same conditions.

Prior to the determination, the coloring degree of each column control unit is checked to confirm that there is no deviation from a preset reference value. After that, for each of the following reagents and component concentrations, data such as the degree of coloring is captured, and calculation, comparison, analysis, etc. are executed.

Concentration (μg / ml) as the judgment reference concentration
It is convenient to obtain the color deviation data in advance and to perform the test based on this calibration curve (coloration degree to density). The judgment is classified into "positive" and "negative" as described above. There is also a judgment based on a significant difference test that is classified into three categories, “positive”, “false positive”, and “negative”.

The hue of the color reaction in various stimulants
If data of the relationship between degree and drug concentration is collected, the test can be carried out quickly, and it is not necessary to explain that it can support a wide range of tests simply by preparing a sample pad in which the type of reagent is changed appropriately. Ah

[0037]

EXAMPLES Example 1 Using a conventional test pad “BIO-RAD” for visual judgment, a test was carried out to apply it to the device of the present invention. It can be said that this "BIO-RAD" has been used as a conventional technique.

In the present invention, the following two types of algorithms can be selected as the determination algorithm. In addition, amphetamine (AMP) 5μ is used as a model of urine (sample) on the sample pad.
Make an aqueous solution containing g / ml and add 4 drops to the sample pad. 1) There is a control section at the top of the coloring reaction section, and it is assumed that the judgment can be made only when it reaches a predetermined coloring state. This is for checking including the case where the reagent charged in the sample pad is not weathered due to weathering.
Obtain a color comparison curve between the control part and the target component in this test. 2) Considering the unevenness of lighting and the variation of the control part,
Calculated by comparing the coloring data with the data of the neighboring uncolored zone. Here, in the non-colored zone, some color shrinkage occurs after the dropping, and the color approaches white with the passage of time.

Next, the color state of amphetamine is tested to reconfirm that this reagent is useful.

The equipment used in the experiment was an optical equipment having an image input section of 400000 pixels and 24 bits. "G" frame (green type) and "gray scale" (achromatic type) were used as the target image frames. For lighting, a white LED was used as a DC drive. Moreover, the calculation processing by the computer is 700 MH as a CPU.
Windows (registered trademark) was used for z and OS.

The tendency of the coloring data changed as follows with the lapse of time after dropping the 5 μg / ml amphetamine aqueous solution.

Here, the control is the reference coloring band at the top of the zone showing the coloring reaction of each component, and the inspection is the coloring data of the bar showing each coloring reaction.

[0043]

[Table 1]

The test results clearly show the following trends: Control value> Target parameter coloring data + adjustment value Constituent content in sample Control value <Target parameter coloring data + adjustment value No sample component In addition, coloring reaction bar data and neighboring background data were examined for each component as a time series curve. . Also in this case, when amphetamine and methamphetamine were used as reagents, and when the tendency of the coloring data turned red with time (the data numerical value became smaller), it became clear that the neighboring uncolored zone data (reference) turned white with time.
In particular, it was found that the 5 μg / ml methamphetamine aqueous solution progressed to a bright red color with the passage of time. <Example 2> A simple operation for acquiring reference calibration data becomes possible. As an example of this operation, a "preparation" button on the main unit (providing the next button on the front or side),
Prepare "Start" button, "Finish" button and "Print" button. First, press the "Prepare" button to turn on the switch. After dropping the reference liquid (for example, 0.5 μg / ml), the sample pad is inserted and set in the apparatus within 30 seconds.
Next, the "start" button is activated. Data of a plurality of measurement values having different preset times is automatically analyzed to obtain data for the target component parameter. (For example, use data for 3, 7, and 10 minutes. During this operation, it is good to call attention by making the "Measuring lamp" blink. After completion of measurement and comparison calculation, "Reference calibration data" is displayed. Is registered in this system (recorded as a database and can be recalled for reproduction). When the deviation is large compared to the data registered (stored) in advance, the system is checked and tuned. "printing"
Operate the button to print out the result.

From the above, acquisition and registration of standard calibration data
(Record) can be done. <Example 3> An actual target sample is prepared, and this sample is tested. Create a sample pad. Insert it into the sample pad insertion part of the device and set it in place. Clear the display on the pre-inspection result display section. After that, automatic measurement is started.
During this time, the "Measuring" lamp blinks. Comparison with calibration data at three points (plural positions) (time series correction, comparison of plural points data) is performed. A comparison result and a total result of each of methamphetamine and amphetamine are calculated (the lamp is displayed during the calculation). In the judgment, when two components are positive, it is a total positive. When any one of the components is positive, a comprehensive suspicion positive. When the two components are negative, the result is a total negative.

<Embodiment 4> Even when the relationship between the reagent concentration and the color data is completely opposite to the above description, the same test can be performed by the data inversion process in the system. For example, in a normal case (color does not contain a stimulant or the like), the color of the sample pad is colorless and white, and a coloring reaction including the stimulant or the like occurs. If the coloring is checked, the fact that the subject took the stimulant can be found through the color of the sample pad.

[0047]

EFFECTS OF THE INVENTION According to the present invention, with respect to the test pad which has a difficulty in determination and has a problem that data cannot be stored for a long period of time, the individual difference of the tester is excluded and the test subject and environmental information are added. Is improved so that the judgment result can be displayed on a screen or the like. Also, by recording and saving the image file, it is possible to establish requirements as evidence data that can be saved for a long time. Further, since a computer can be used, there is an advantage that a huge amount of data can be collected and search processing can be performed.

[Brief description of drawings]

FIG. 1 shows a perspective view of the device of the present invention.

FIG. 2 is an explanatory diagram showing a surface of a sample pad.

FIG. 3 is a block diagram of the device of the present invention.

[Explanation of symbols] 10 Device body 11 Memory card slot 12 Sample pad insertion port 20 Sample pad setting section (reagent reaction section) 30 Lighting system 32 Image input section 33 Image display section 34 Image converter 35 Image processing unit 36 Record display 37 Judgment output section 38 Integrated control unit 40 power supply

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Mitsuteru Kusuda             5-7-18 Higashi-Nippori, Arakawa-ku, Tokyo Cosmopa             Kurville Japan Bio-Rad Laboratories             Within Co., Ltd. (72) Inventor Yoshinori Ide             1-1-14 Shinjuku, Shinjuku-ku, Tokyo Asahi Techney             On Co., Ltd. (72) Inventor Kunihiro Asashino             1-1-14 Shinjuku, Shinjuku-ku, Tokyo Asahi Techney             On Co., Ltd. F term (reference) 2G045 AA15 AA37 CB03 DA80 FA11                       FA18 GC12 HA09 HA14 JA01

Claims (8)

[Claims] 1. A character information input unit for attribute / environmental information of a subject, a sample pad setting unit (reagent reaction unit) into which urine of the subject can be injected, an illumination system, and a CCD image sensitive to the illumination system. Input unit, image processing unit, RGB image analysis unit, determination algorithm processing unit, determination output unit, the character information, the image information,
An automatic drug determination device for automatically determining and recording a drug test including a storage unit for determination records and an integrated control unit. 2. The automatic medicine as set forth in claim 1, which is an illumination means including an RBG three-color component, comprising an illumination system capable of automatically detecting an optimal illumination wavelength and automatically adjusting to the optimal illumination wavelength. Judgment device. 3. When coloration or discoloration occurs due to the reaction between the sample and the reagent, the site (inside the reaction area) where the coloration or discoloration occurs in the sample pad does not sufficiently react with the sample and the reagent. Part (peripheral part) and at least two parts of the color tone are automatically detected, and deviation data of these are detected,
The drug automatic determination device according to claim 1, further comprising an image processing algorithm processing unit for image determination for performing comparison calculation. 4. Detecting at least two points of blank data (control) before sample injection and a site (inside said reaction area) where coloration or discoloration should occur after sample injection, and compare and calculate these to determine the significance. The automatic drug determination device according to claim 1, further comprising image determination means for determining a difference. 5. Coloration in the reaction zone after injecting a sample by using the blank data (control) and a time-series change model in which a standard color tone change until the reaction between the sample and the reagent is completed is converted into data. Alternatively, the automatic drug determination device according to claim 1 or 4, further comprising image determination means for comparing and calculating the discoloration and the time-series change model to determine a significant difference. 6. A three-color RBG component of image data, a grayscale model, and an infrared ray based on the color tone (wavelength) of a site (inside the reaction area) where coloration or discoloration has occurred in the sample pad due to the reaction between the sample and the reagent. Either of the states of the regions, alone or by adding the correlations to each other, to optimize the illumination state by controlling the color tone, and to optimize the illumination state by performing an analytical calculation by color separation in the color image. The automatic drug determination device according to claim 1, wherein the automatic drug determination device is selected. 7. The automatic drug determination device according to claim 1, wherein in the test algorithm, one of population comparison (magnitude relation of variance values) and average value comparison of luminance distribution in the coloring reaction is selected. 8. When inserting the sample pad into the device,
The automatic drug determination device according to claim 1, wherein the sample pad is provided with a position control mechanism capable of highly accurately determining the position by a multi-pole method.