JP2003098069A - Light transmittable plate for holding liquid and method of manufacturing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a light transmittable plate for holding the liquid capable of easily performing the optical measurement of the liquid in a condition that a small amount of liquid is held on a surface. SOLUTION: This light transmittable plate 1 for holding liquid on the surface is provided with plural liquid holding grooves 3 on the surface of the light transmittable plate 2. The liquid can be quickly expanded by capillary phenomenon in the grooves 3 by dropping the liquid on the surface of the light transmittable plate 1 for holding the liquid. When the light transmittable plate 1 is vertically stood, it holds the liquid, so that the liquid dripping is hardly found.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a liquid-transmitting light-transmitting plate in which a liquid is spread and held when a liquid is dropped on the surface and a manufacturing method thereof, and particularly to liquid analysis, medical examination, water-solubility. The present invention relates to a liquid-holding light-transmitting plate that can be suitably used for testing proteins and the like, and a method for manufacturing the same.

[0002]

2. Description of the Related Art Conventionally, in analyzing the components of water-soluble proteins and the like, it has been common to measure the absorbance, transmittance, etc. of a liquid sample with a spectrophotometer. The measurement was carried out with the mold cell held.

Further, when the amount of the liquid to be measured is small, it is difficult to perform the measurement using a container-type cell, and the liquid is dropped on a transparent plate such as glass to form a film. By doing so, the optical characteristics of the liquid were measured.

US Pat. No. 4,402,927, US Pat. No. 4,432,956, US Pat. No. 4
As disclosed in No. 610863 specification, etc., having a characteristic such as low refractive index, a liquid is dropped onto a transparent porous body typified by porous silica airgel or silica gel,
A method has also been considered in which a droplet is retained by absorbing it, and its optical characteristics are measured.

These techniques have been utilized, for example, in the case where a pyrirubin test (test for jaundice) is performed by irradiating a plate with plasma dropped on it with yellow light and measuring its optical characteristics.

[0006]

However, when a plate made of glass or the like is used, when the liquid is dropped on the plate, if the plate is stood vertically, the liquid flows and it is difficult to perform optical measurement. Met.

When silica aerogel is used, the portion where the liquid is dropped causes cloudiness, and when silica gel is used, the portion where the liquid is dropped is cracked.
The optical measurement cannot be easily performed.

The present invention has been made in view of the above points, and a liquid-transmitting light-transmitting plate for holding a liquid capable of easily performing optical measurement of the liquid while holding a small amount of the liquid on the surface thereof. It is intended to provide a manufacturing method.

[0009]

A liquid-holding light-transmitting plate according to the present invention is a liquid-holding light-transmitting plate 1 for holding a liquid on a surface thereof, and a liquid-holding light-transmitting plate 2 is provided on a surface of the plate 2. It is characterized in that a plurality of liquid holding grooves 3 are formed.

The invention of claim 2 is characterized in that, in claim 1, the plurality of grooves 3 are formed in a lattice shape.

The invention of claim 3 is characterized in that, in claim 1 or 2, the inner surface of the groove 3 is roughened.

The invention of claim 4 is characterized in that, in any of claims 1 to 3, the inner surface of the groove 3 is made hydrophilic.

The invention of claim 5 is characterized in that, in any one of claims 1 to 4, the groove 3 is formed so that the depth thereof is 15 μm or more.

The invention of claim 6 is characterized in that in any one of claims 1 to 5, a hydrophilic film is formed on the inner surface of the groove 3.

The invention of claim 7 is characterized in that, in any of claims 1 to 6, the inner surface of the groove 3 is made hydrophilic by subjecting it to plasma treatment.

The invention of claim 8 is characterized in that, in any one of claims 1 to 7, it is formed for liquid analysis.

The invention of claim 9 is characterized in that, in any one of claims 1 to 8, it is formed for medical examination.

The invention of claim 10 relates to claims 1 to 9.
In any one of the above, it is formed to be used for testing a water-soluble protein.

An eleventh aspect of the present invention is a method for producing a liquid-holding light-transmitting plate according to any one of the first to tenth aspects of the present invention. It is possible to form the groove 3 by coating the surface of the plate 2 having light transparency except the portion where the groove 3 is formed, and then contacting the powder to cut the uncoated portion. It is a feature.

[0020]

BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the present invention will be described below.

The material for the liquid-holding light-transmitting plate 1 of the present invention is not particularly limited as long as it has light-transmitting properties, but when it is used for measuring the optical characteristics of a liquid, for example, UV spectrum analysis. 380-
It is preferable to use a material having light transmittance in the light wavelength region used for measurement, such as a material having light transmittance in the wavelength region of 780 nm. Here, having light transmittance means having a light transmittance of 50 to 100%. Specific materials include glass and plastics such as polyethylene terephthalate, polystyrene, acrylic, and polycarbonate.

In the liquid-holding light-transmitting plate 1 of the present invention, the liquid-holding light-transmitting plate is formed by forming a plurality of grooves 3 on the surface of the plate 2 made of the above-mentioned light-transmitting material. The liquid dropped on the surface of the plate 1 can be spread on the surface of the liquid-holding light-transmitting plate 1 by a capillary phenomenon, and the liquid can be held on the surface of the liquid-holding light-transmitting plate 1.

The pattern of the grooves 3 formed on the surface of the liquid-holding light-transmitting plate 1 depends on the analysis method, and the amount of the liquid to be analyzed, the desired development area of the liquid, its shape, etc. It is set appropriately according to.

For example, when it is desired to selectively spread the liquid on the surface of the liquid-holding transparent plate 1 in one direction along the surface of the liquid-holding transparent plate 1,
As shown in FIG. 1, it is preferable to form a plurality of parallel and parallel grooves 3 on the surface of the liquid holding light-transmitting plate 1 to form the grooves 3 in a striped pattern. When it is desired to spread the liquid on the surface of No. 1 equally in all directions along the surface of the liquid-holding transparent plate 1, as shown in FIG. 2, it is parallel and parallel to the surface of the liquid-holding transparent plate 1. A plurality of different grooves 3 are formed,
It is preferable that a plurality of grooves 3 orthogonal to the grooves 3 are formed in parallel and in parallel to form the grooves 3 in a grid pattern.

The depth of the groove 3 is limited by the method of forming the groove 3 such as sand blasting, which will be described later, but it is preferably formed in the range of 10 to 100 μm, and the depth is particularly 15 μm. When formed as described above, the liquid can be easily spread, the effect of preventing dripping is enhanced, and a sufficient amount of liquid to be retained for analysis can be obtained.

The aperture ratio of the groove 3 on the surface of the liquid-holding transparent plate 1 is preferably 20 to 80%. Here, the opening ratio of the groove 3 is the ratio of the area of the portion where the groove 3 is formed to the total surface area of the surface of the liquid holding light-transmitting plate 1 where the groove 3 is formed. If the opening ratio of the groove 3 is too large, the liquid can be easily spread on the surface of the liquid-holding light-transmitting plate 1, but dripping occurs when the liquid-holding light-transmitting plate 1 is erected vertically. It will be easier. Further, if the opening ratio of the groove 3 is too small, it becomes difficult to spread the liquid on the surface of the liquid holding light transmissive plate 1. However, the opening ratio of the groove 3 is appropriately set according to the viscosity of the liquid, the wettability between the material of the liquid-holding light-transmitting plate 1 and the liquid, and for example, the sample liquid is plasma. When the viscosity is 1.6 to 2.2 cP, the aperture ratio is 70
It is preferably in the range of ˜90%. At this time, it is preferable that the width of the groove 3 is formed in the range of 10 to 200 μm, and the interval between the adjacent grooves 3 is 10 to 200 μm.
It is preferable to form so that.

Further, the liquid-holding light-transmitting plate 1 is subjected to a treatment for improving the wettability (affinity) with the liquid, whereby the development of the liquid can be further facilitated and the dripping can be prevented. preferable.

For example, by roughening the inner surface of the groove 3 formed in the liquid-holding light-transmitting plate 1, when the liquid is dropped on the surface of the liquid-holding light-transmitting plate 1, the liquid and the groove 3 are formed. The affinity with the inner surface can be improved. This roughening treatment can be performed by bringing powder for cutting such as silicon carbide into contact with the inner surface of the groove 3 by a method such as sandblasting. The surface roughness of the inner surface of the groove 3 at this time is preferably 0.01 to 10 μm.

The inner surface of the groove 3 formed in the liquid holding light-transmitting plate 1 is subjected to plasma treatment and UV-light irradiation.
By applying the O ₃ treatment, the inner surface of the groove 3 can be made hydrophilic. In this case, the affinity between the liquid and the inner surface of the groove 3 can be improved particularly when an aqueous liquid is dropped on the surface of the liquid holding light-transmitting plate 1.

Here, the plasma treatment can be performed under appropriate conditions. For example, plasma is generated using oxygen at atmospheric pressure or reduced pressure, and the inner surface of the groove 3 is exposed to this oxygen plasma, so that the surface of the groove 3 is exposed. The organic impurities adhering to the resin can be removed, and a functional group containing oxygen is formed on the inner surface of the groove 3, so that the hydrophilicity is improved. At the same time, the adhesion is also improved by forming fine irregularities at the molecular level.

In the UV-O ₃ treatment, for example, oxygen in the air is ozoned by a low-pressure mercury lamp or an excimer lamp, and the oxygen radicals generated at that time decompose organic matter on the inner surface of the groove 3 to make it hydrophilic. It will improve.

Further, a coating film is formed on the inner surface of the groove 3 formed in the liquid holding light-transmitting plate 1 with a hydrophilic coating material or the like to make the inner surface of the groove 3 hydrophilic.
It is also possible to improve the affinity between the liquid and the inner surface of the groove 3 when the aqueous liquid is dropped on the surface of the liquid-holding transparent plate 1. In this case, as the coating material, an appropriate material is used, but it is preferable to use a silicone resin or the like. As a coating method, an appropriate method such as a spray method or a roll coater method can be adopted.
At this time, the thickness of the coating film of the coating material is 0.1 to
The range is preferably 1.0 μm.

A plate 2 made of a light-transmissive material
When the liquid-holding light-transmitting plate 1 is obtained by forming the groove 3 in the surface, after coating the surface of the plate 2 excluding the portion where the groove 3 is formed, the portion not covered by a method such as sandblasting. The groove 3 can be formed by bringing powder for cutting such as silicon carbide into contact therewith and cutting. At this time, when the surface of the plate 2 is coated, for example, the surface of the plate 2 is coated with a photosensitive resin sheet, a resist solution or the like, and a desired pattern is exposed and then developed to form the groove 3. The resist film can be formed except for. When forming such a resist film, it is preferable to form a film having high hardness that has durability against sandblasting. Further, the coating of the plate 2 is not limited to such a method, and it can be coated with, for example, a metal mask made of stainless steel or the like, and is coated with a material having durability in the sandblasting process. If so, the material is not particularly limited. Also, the thickness of the coating is appropriately set depending on the material of the coating.

When the groove 3 is formed by cutting with powder for cutting in this way, the inner surface of the formed groove 3 is roughened, and thus the inner surface of the groove 3 can be roughened. It is a thing.

Such a liquid-holding light-transmitting plate 1
Is capable of rapidly developing the liquid by the capillary phenomenon in the groove 3 by dropping the liquid on the surface of the liquid-holding light-transmitting plate 1, and standing the liquid-holding light-transmitting plate 1 vertically. In such a case, the liquid-holding light-transmitting plate 1 can hold the liquid and prevent dripping from occurring. Therefore, it can be suitably used for holding and fixing a liquid sample when performing optical measurement such as measuring the absorbance and transmittance of a small amount of the liquid sample with a spectrophotometer. In particular, in the field of medical tests and in the case of measuring water-soluble proteins, a small amount of sample is often obtained, and even in such a case, the sample can be easily measured.

[0036]

EXAMPLES The present invention will be described in detail below with reference to examples.

(Example 1) 20 mm on one surface of the plate 2 made of soda lime glass of 50 mm x 50 mm
A photosensitive resin sheet having a thickness of 20 μm (manufactured by Nippon Synthetic Chemical Industry Co., Ltd.) was adhered to a region of × 20 mm by a roll pressing method, and exposed through a pattern mask using an exposure device (manufactured by Adtech Engineering Co., Ltd.). later,
Remove the unexposed part by developing with a developing solution,
A resist film was formed.

Then, the portion of the plate 2 on which the resist film is formed is subjected to sandblasting with a spraying pressure of 2 kg / cm ² using silicon carbide powder having a particle size of 1 to 3 μm, and then the resist film is baked at 500 ° C. Removed.

As a result, the width is 60 μm and the depth is 10 to 15.
A plurality of grooves 3 each having a surface roughness of 0.4 μm and an inner surface having a surface roughness of 0.4 μm are formed in a lattice shape as in the case shown in FIG. 2 and the opening ratio of the grooves 3 is 75%. Got

(Embodiment 2) Grain size 1 by sandblasting
A liquid-holding light-transmitting plate 1 was obtained in the same manner as in Example 1 except that the surface roughness of the inner surface of the groove 3 was set to 1 μm by using 1 to 17 μm of silicon carbide powder.

Example 3 Except that the width of the groove 3 was 100 μm, the depth was 15 to 30 μm, and the aperture ratio was 86%.
In the same manner as in Example 1, the liquid holding optical transparent plate 1
Got

Example 4 A silicone resin (trade name “Frussela R” manufactured by Matsushita Electric Works, Ltd.) was applied to the inner surface of the groove 3 by a roll coater to form a coating film having a thickness of 0.2 μm. A liquid-holding light-transmitting plate 1 was obtained in the same manner as in Example 3.

(Example 5) The inner surface of the groove 3 was subjected to AI plasma treatment in an atmospheric pressure atmosphere under the conditions of an output of 700 W and a scan speed of 4 mm by a plasma treatment apparatus manufactured by Matsushita Electric Machinery & Vision Co., Ltd. Example 3 except
In the same manner as described above, a liquid holding light transmissive plate 1 was obtained.

(Comparative Example 1) No treatment 5
A 0 mm × 50 mm plate made of soda lime glass was used as a liquid-transmitting light-transmitting plate.

(Comparative Example 2) A plate made of soda lime glass of 50 mm x 50 mm was not grooved, but its surface was coated with a silicone resin by a roll coater (trade name "Frussela R" manufactured by Matsushita Electric Works, Ltd.). )) Was applied to form a coating film having a thickness of 0.2 μm to obtain a liquid-holding light-transmitting plate.

(Comparative Example 3) A plate made of soda lime glass of 50 mm x 50 mm was not grooved, and its surface was 700 W with a plasma processing apparatus manufactured by Matsushita Electric Machine and Vision Co., Ltd. and a scanning speed. Four
AI plasma treatment was performed in an atmosphere of atmospheric pressure under a condition of mm to obtain a liquid-holding light-transmitting plate.

(Evaluation Test) Liquid (plasma, manufactured by Sanko Junyaku Co., Ltd., trade name "EXE Liquid" was applied to the surface of the liquid-holding light-transmitting plate obtained in each of the examples and comparative examples.
d 5 normal ”, product number“ SC1313 ”) 0.03 cm ³ and the time required for the liquid to spread and stop flowing on the liquid-holding transparent plate,
And the area of the region where the liquid spreads at this time was measured.

Further, the liquid-holding light-transmitting plate thus developed by dropping the liquid was erected vertically and observed for the occurrence of liquid dripping.

The results are shown in Table 1.

[0050]

[Table 1]

[0051]

As described above, the liquid-holding light-transmitting plate according to the present invention is a liquid-holding light-transmitting plate that holds a liquid on its surface, and a plurality of light-transmitting plates are provided on the surface of the plate. In order to form the liquid holding groove, by dropping the liquid on the surface of the liquid holding light transmissive plate, it is possible to rapidly develop the liquid due to the capillary phenomenon in the groove, When the plate is erected vertically, the liquid-holding light-transmitting plate can hold the liquid and prevent dripping from occurring.

Further, in the invention of claim 2, since the plurality of grooves are formed in a lattice shape in claim 1, the liquid dropped on the surface can be spread and held in all directions along the surface. is there.

Further, in the invention of claim 3, in the invention of claim 1 or 2, since the inner surface of the groove is roughened, the affinity with the liquid is improved and the liquid dripped on the surface can be spread more quickly. In addition to being able to do so, the holding power of this liquid is improved, and dripping is further less likely to occur.

The invention according to claim 4 is the method according to any one of claims 1 to 3, wherein the inner surface of the groove is hydrophilic.
The affinity with an aqueous solution liquid is improved, the aqueous solution liquid dropped on the surface can be more rapidly developed, and the holding power of this liquid is improved, so that dripping is further less likely to occur. is there.

Further, in the invention of claim 5 according to any one of claims 1 to 4, since the depth of the groove is formed to be 15 μm or more, the liquid can be easily spread and the effect of preventing the liquid dripping is great. In addition, the liquid holding amount required for the analysis can be sufficiently obtained.

Further, in the invention of claim 6 according to any one of claims 1 to 5, since a hydrophilic film is formed on the inner surface of the groove, the aqueous liquid dropped onto the surface can be spread more quickly. In addition to being able to do so, the holding power of this liquid is improved, and dripping is further less likely to occur.

Further, in the invention of claim 7 according to any one of claims 1 to 6, since the inner surface of the groove is hydrophilized by performing plasma treatment, the aqueous liquid dropped on the surface is spread more quickly. In addition, the holding power of the liquid can be improved and dripping can be further suppressed.

Further, according to the invention of claim 8, in any one of claims 1 to 7, since it is formed for liquid analysis, it is possible to easily hold and fix even a small amount of liquid sample. It is possible to easily perform optical measurement such as measuring the rate with a spectrophotometer.

In addition, since the invention of claim 9 is any one of claims 1 to 8, since it is formed for medical examination, even a small amount of liquid sample can be easily held and fixed. Even if it can only be obtained, the analysis can be easily performed.

The invention of claim 10 relates to claims 1 to 9.
In any of the above, since it is formed for testing water-soluble proteins, it is possible to easily hold and fix even a small amount of liquid sample, so even if only a small amount of sample can be obtained, it is easy to analyze. Is what you can do.

A method for producing a liquid-holding light-transmitting plate according to claim 11 of the present invention is the method for producing a liquid-holding light-transmitting plate according to any one of claims 1 to 10. After coating the surface of the plate that has permeability except the portion where the groove is formed, the groove is formed by cutting the uncoated portion by contacting with the powder, so that the desired pattern is formed. The groove can be easily formed, and the inner surface of the groove can be roughened simultaneously with the formation of the groove.

[Brief description of drawings]

FIG. 1 is a plan view showing an example of an embodiment of the present invention.

FIG. 2 is a plan view showing another example of the embodiment of the present invention.

[Explanation of symbols]

1 Light-transmitting plate for holding liquid 2 plates 3 grooves

Continued front page    (72) Inventor Yoshihiro Ito             1048, Kadoma, Kadoma-shi, Osaka Matsushita Electric Works Co., Ltd.             Inside the company (72) Inventor Saori Urata             2-2-5-606 Nishi, Hamazura Ishizu Town, Sakai City, Osaka Prefecture F term (reference) 2G045 BB21 BB48 FA11 JA07                 2G057 AA01 AB06 AC01 BA01 BA03                       BB01 BB06 CB03

Claims (11)

[Claims] 1. A liquid-holding light-transmitting plate for holding a liquid on its surface, wherein a plurality of liquid-holding grooves are formed on the surface of the plate having light-transmitting properties. Light transmissive plate for. 2. The liquid-holding light-transmitting plate according to claim 1, wherein the plurality of grooves are formed in a grid pattern. 3. The liquid transmissive plate for holding liquid according to claim 1, wherein the inner surface of the groove is roughened. 4. The liquid transmissive plate for holding liquid according to claim 1, wherein the inner surface of the groove is hydrophilized. 5. The liquid transmissive plate for holding liquid according to claim 1, wherein the groove has a depth of 15 μm or more. 6. The liquid-transmitting light-transmitting plate according to claim 1, wherein a hydrophilic film is formed on the inner surface of the groove. 7. The liquid transmissive plate for holding liquid according to claim 1, wherein the inner surface of the groove is made hydrophilic by plasma treatment. 8. The liquid-holding light-transmitting plate according to any one of claims 1 to 7, which is formed for liquid analysis. 9. The liquid-transmitting light-transmitting plate according to claim 1, which is formed for medical examination. 10. The liquid-transmitting light-transmitting plate according to any one of claims 1 to 9, which is formed for testing water-soluble proteins. 11. A method for manufacturing the liquid-transmitting light-transmitting plate according to claim 1.
It is characterized in that after coating the surface of the plate having optical transparency except the portion where the groove is formed, the groove is formed by cutting the uncoated portion by contacting with the powder. A method of manufacturing a light-transmitting plate for holding a liquid.