JP2003040724A - Cosmetic and cosmetic preservative - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide both a cosmetic having high safety and excellent shelf life without being influenced with cosmetic components and a cosmetic preservative. SOLUTION: This cosmetic is obtained by formulating ε-polylysine and/or its salt and an organic acid salt with the cosmetic. The cosmetic preservative is prepared by combining the ε-polylysine and/or its salt with the organic acid salt.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to cosmetics and cosmetic preservatives.

[0002]

2. Description of the Related Art In cosmetics, synthetic preservatives such as benzoic acids, sorbic acids, benzoic acid esters, and chlorhexidine gluconate are used for the purpose of preventing spoilage by microorganisms and accompanying deterioration in quality. . Among them, parahydroxybenzoic acid esters (hereinafter referred to as “paraben”) are most widely used.

[0003]

Paraben, however, has a low antibacterial effect against Gram-negative bacteria, and has an antibacterial effect due to amphoteric surfactants such as phospholipids commonly used as cosmetic ingredients and nonionic surfactants. Has problems such as decrease in skin irritation, skin irritation and allergenicity. There is a demand for cosmetics which have a sufficient antiseptic ability regardless of the bacterial species that cause microbial contamination, have a small decrease in the antiseptic ability even in the presence of various cosmetic ingredients, and have excellent safety.

As a highly safe cosmetic product, a cosmetic product containing ε-polylysine, which is a food preservative derived from a natural product, and / or a salt thereof as a preservative has been proposed (Japanese Patent Laid-Open Nos. 5-64608 and 5-60808). 11-228308, JP 2000-239118). However, ε-polylysine and / or a salt thereof is an anionic substance which is a cosmetic ingredient, for example, a mucopolysaccharide such as hyaluronic acid or chondroitin sulfate, a mucopolysaccharide such as xanthan gum or sodium carboxymethylcellulose, and polyoxyethylene lauryl ether. It may adsorb or form a salt with an anionic surfactant such as sodium sulfate / ethylene oxide adduct or sodium lauryl sulfosuccinate, resulting in turbidity or precipitation, or inhibition of antibacterial effect.

[0005]

The present inventor has conducted intensive studies in view of the above-mentioned problems of the prior art. As a result, a cosmetic containing ε-polylysine and / or a salt thereof and an organic acid salt is unlikely to be decomposed or deteriorated by microorganisms without being adsorbed by a cosmetic component, salt formation, and antibacterial inhibition. thing. Furthermore, they have found that the cosmetic product has less rough skin and less skin irritation, and completed the present invention based on this finding.

The present invention has the following configurations (1) to (16). (1) A cosmetic containing ε-polylysine and / or a salt thereof and an organic acid salt.

(2) The cosmetic according to the above item 1, wherein the organic acid salt is at least one selected from citrate, malate, lactate, acetate, succinate, and phosphate.

(3) The cosmetic according to the above item 1, further containing at least one selected from nonionic surfactants, amphoteric surfactants, and anionic surfactants.

(4) In addition, the first component containing an organic acid
Item 4. The cosmetic according to any one of Items 3 to 3.

(5) The cosmetic according to the above item 4, wherein the organic acid is at least one selected from citric acid, malic acid, lactic acid, acetic acid, succinic acid, and phosphoric acid.

(6) The first item containing a pH buffer solution
The cosmetic according to any one of item 5.

(7) The pH buffer solution is a pH buffer solution comprising one or more acids selected from citrate, citric acid, malic acid, lactic acid, acetic acid, succinic acid, and phosphoric acid, and salts thereof. The cosmetic according to item 6.

(8) A cosmetic preservative containing ε-polylysine and / or a salt thereof and an organic acid salt.

(9) The cosmetic preservative according to the above item 8, wherein the organic acid salt is at least one selected from citrate, malate, lactate, acetate, succinate, and phosphate. .

(10) The cosmetic preservative according to the above item 8 or 9, further comprising an organic acid.

(11) Organic acids are citric acid, malic acid,
11. The cosmetic preservative according to the above item 10, which is one or more selected from lactic acid, acetic acid, succinic acid, and phosphoric acid.

(12) The eighth composition containing a pH buffer solution
Item 11. The cosmetic preservative according to any one of Items 11 to 11.

(13) The pH buffer solution is a pH buffer solution comprising one or more acids selected from citrate, citric acid, malic acid, lactic acid, acetic acid, succinic acid, and phosphoric acid and salts thereof. The cosmetic preservative according to item 12.

(14) The cosmetic preservative according to any one of items 8 to 13 which further contains a surfactant and / or an amino acid.

(15) The surfactant is a monoester of glycerin and a fatty acid having 8 to 12 carbon atoms, and the degree of polymerization is 2 to 9
And monoesters of polyglycerol with fatty acids having 8 to 16 carbon atoms, monoesters and diesters of sucrose with fatty acids having 8 to 16 carbon atoms, and etherified products of sugar with fatty acids having 8 to 16 carbon atoms Item 15. The cosmetic preservative according to Item 14, which is one or more selected from the following.

(16) The cosmetic preservative according to the above item 14, wherein the amino acid is at least one selected from glycine and alanine.

(17) A cosmetic containing the cosmetic preservative according to any one of items 8 to 16.

[0023]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below. The ε-polylysine used in the present invention can be used in the present invention regardless of the method obtained, but specifically, Streptomyces alpras described in Japanese Patent No. 1245361. The composition of Subspecies lysinopolymeris has the following composition: glucose 5% by weight, yeast extract 0.5% by weight, ammonium sulfate 1% by weight, dipotassium hydrogenphosphate 0.08% by weight, potassium dihydrogenphosphate 0.136% by weight, 0.05% by weight of magnesium sulfate heptahydrate, 0.004% by weight of zinc sulfate heptahydrate, and 0.03% by weight of iron sulfate heptahydrate,
An example is ε-polylysine obtained by culturing in a medium whose pH is adjusted to 6.8 and separating and collecting ε-polylysine from the obtained culture.

The ε-polylysine used in the present invention may be free ε-polylysine, and may be a ε-polylysine formed from ε-polylysine and an inorganic acid such as hydrochloric acid, sulfuric acid and phosphoric acid. Inorganic acid salts and organic acid salts of ε-polylysine formed by ε-polylysine and organic acids such as acetic acid, propionic acid, fumaric acid, malic acid, citric acid, maleic acid, adipic acid, gluconic acid, and lactic acid. Or

Saturated fatty acid salts of ε-polylysine formed by medium- and long-chain saturated fatty acids such as caproic acid, lauric acid, and stearic acid, and ε-polylysine, oleic acid, linoleic acid, and arachidonic acid. It may be an unsaturated fatty acid salt of ε-polylysine formed by medium-chain and long-chain unsaturated fatty acids and ε-polylysine.
After this, salts of the above-mentioned various acids with ε-polylysine,
And free ε-polylysine are collectively referred to as “EPL”.

The EPL content in the cosmetic of the present invention is not particularly limited, but it is preferably in the range of 0.0001 to 5% by weight based on the cosmetic.

The organic acid salt used in the present invention is not particularly limited, but among the organic acid salts, citrate, malate, lactate, acetate, succinate, and phosphate are: It can be preferably used in the present invention.

The content of the organic acid salt contained in the cosmetic of the present invention is not particularly limited, but it is preferably in the range of 0.005 to 20% by weight based on the cosmetic.

The cosmetic of the present invention preferably contains at least one selected from nonionic surfactants, amphoteric surfactants, and anionic surfactants.

The nonionic surfactant used in the present invention is not particularly limited, but for example, ester type nonionic surfactants such as glycerin fatty acid ester, sorbitan fatty acid ester, and sucrose fatty acid ester, and polyoxy ester. Ether type nonionic surfactants such as ethylene alkyl ether, polyoxyethylene alkylphenyl ether and polyoxyethylene polyoxypropylene block polymer, ester ether type nonionic surfactants such as fatty acid polyethylene glycol and fatty acid polyoxyethylene sorbitan, fatty acid Examples thereof include alkanolamide-type nonionic surfactants such as alkanolamides, and alkyl glycoside-type nonionic surfactants such as lauric acid glycosides.

The amphoteric surfactant used in the present invention is not particularly limited, but amphoteric surfactants produced by chemical synthesis, for example, betaine-type amphoteric surfactants such as alkyl betaine and fatty acid amidopropyl betaine. Agent, imidazoline-type amphoteric surfactant such as 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, glycine-type amphoteric surfactant such as alkyldiethylenetriaminoacetic acid, and amine oxide-type such as alkylamine oxide Examples thereof include amphoteric surfactants.

In addition to these, naturally occurring amphoteric surfactants such as phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylserine, N
Phospholipids such as acylphosphatidylethanolamine, sphingomyelin, plasmalogens, and mixtures thereof soybean lecithin, egg yolk lecithin,
Milk lecithin etc. can be mentioned.

The anionic surfactant used in the present invention is not particularly limited, but for example, fatty acid potassium, fatty acid sodium, N-acyl sarcosine salt, N-
Carboxylate type anionic surfactants such as acylglutamates and α-sulfo fatty acid ester salts, dialkyl sulfosuccinates, alkyl sulfonates, α-
Sulfonate type anionic surfactants such as olefin sulfonate, alkylbenzene sulfonate, alkylnaphthalene sulfonate, and N-methyl-N-acyl taurine salt, alkyl sulfate ester salt, polyoxyethylene alkyl ether sulfate ester Salt, sulfated oil,
Sulfated fatty acid esters, sulfate ester type anionic surfactants such as sulfated olefins, and phosphoric acid ester type anionic surfactants such as polyoxyethylene alkylphenyl ether phosphate can be mentioned. .

The content of at least one selected from nonionic surfactants, amphoteric surfactants, and anionic surfactants in the cosmetics of the present invention is not particularly limited, but it is not limited thereto. On the other hand, it is preferably in the range of 0.005 to 80% by weight.

It is preferable to add an organic acid to the cosmetic of the present invention. The organic acid used in the present invention is not particularly limited, but among the organic acids, citric acid, malic acid, lactic acid, acetic acid, succinic acid, and phosphoric acid can be preferably used in the present invention.

The content of the organic acid contained in the cosmetic of the present invention is not particularly limited, but it is preferably 0.005 to 20% by weight based on the cosmetic.

The cosmetic of the present invention preferably contains a pH buffer solution. Thereby, a high antiseptic ability can be exhibited even in a pH range close to the pH of human skin (pH 5.4 to 6.2). The pH buffer solution used in the present invention is not particularly limited, but is a weakly acidic substance-weakly basic substance, a weakly acidic substance-strongly basic substance,
Any pH buffer solution can be used as long as it is a pH buffer solution composed of a strongly acidic substance and a weakly basic substance.

In particular, citric acid, malic acid, lactic acid, acetic acid,
A pH buffer solution comprising one or more acids selected from succinic acid and phosphoric acid and salts thereof (citric acid-citrate buffer solution, malic acid-malate salt buffer solution, lactic acid-lactate buffer solution, Acetic acid-acetate buffer etc.) can be preferably used in the present invention because they are particularly excellent in antiseptic ability, safety and cost when applied to cosmetics.

In the present invention, a pH buffer solution adjusted in advance may be added to the cosmetics of the present invention. A pH buffer solution may be formed in the cosmetic product by adding an organic acid salt contained in the cosmetic product and an aqueous organic acid solution capable of forming a buffer solution to the cosmetic product of the present invention at an appropriate ratio.

The content ratio of the pH buffer solution contained in the cosmetic of the present invention is not particularly limited, but it is preferably in the range of 0.005 to 99.9% by weight with respect to the cosmetic.

The cosmetics of the present invention may contain various cosmetic raw materials depending on the purpose, as long as the effects of the present invention are not impaired. Examples of the cosmetic raw material include, but are not limited to, powders mixed for the purpose of a cationic surfactant, an oil component, a colorant and the like.

As the cationic surfactant, for example,
Examples thereof include alkyl trimethyl ammonium salt, dialkyl dimethyl ammonium salt, alkyl pyridinium salt, and benzalkonium chloride.

Any oil can be used in the present invention as long as it can be used in ordinary cosmetics. Vegetable oils such as olive oil, jojoba oil, castor oil, rice bran oil and coconut oil, and animal oils such as squalane and beef tallow. , Lanolin, etc., synthetic oils such as silicone oil, polyisobutene, fatty acid esters, fatty acid glycerin, etc., waxes such as beeswax, wax, candelilla wax, carnauba wax, etc., hydrocarbons as liquid paraffin, ceresin, microcrystalline wax, vaseline, etc., Higher alcohols include cetanol, stearyl alcohol, octyldodecanol, etc., higher fatty acids include stearic acid, lauric acid, myristic acid, oleic acid, etc., and other oils such as silicone resin, silicone rubber, perfluoroether. And the like.

The powder to be blended for the purpose of a colorant or the like is one which is usually used in cosmetics and includes organic dyes (blue 1
No. 3, Green No. 3, Red No. 202, Red No. 227, Yellow,
No. 4 etc. and its lake), inorganic pigments (iron oxide, titanium oxide, chromium oxide, zinc oxide, etc.), extender pigments (serisite, mica, talc, nylon powder, cellulose powder, silicon powder, alkyl polyacrylate, Calcium phosphate, boron nitride, etc.), pearl (titanium oxide treated mica, titanium oxide.iron oxide treated mica,
Titanium oxide. (Navy blue mica, etc.), and natural pigments such as chlorophyll and β-carotene. In the present invention, one or more of these may be selected and used as long as the effects of the present invention are not impaired.

The powder may be subjected to surface treatment and / or compounding for the purpose of improving the hydrophobicity of the powder, reducing the catalytic activity, and improving the lubricity. This surface treatment,
Examples of the substance used for complexing include silicic acid anhydride, titanium oxide, nylon, alkyl polyacrylate, fluorine compounds, metal soaps, fats and oils, and fatty acid esters.

In addition to the above components, gums, mucopolysaccharides, plant extracts, animal extracts, natural water-soluble compounds and derivatives thereof are added to the cosmetics of the present invention as long as the stability of the system is not impaired. , Chelating agents, antioxidants,
Moisturizers, lower alcohols, polyhydric alcohols, fragrances, cooling agents, pH adjusters, UV inhibitors, whitening agents, anti-inflammatory agents, anti-aging agents, anti-stain agents, inorganic salts, inorganic acids, inorganic bases, and sugars. You may mix quality etc.

Further, the cosmetic of the present invention contains a bamboo extract, as long as the effect and safety of the present invention are not impaired.
Allyl mustard oil, protamine, tea extract, grapefruit seed extract, lysozyme, chitosan, hinokitiol and other naturally occurring antibacterial substances, and substances that reinforce the antibacterial effect of EPL, for example, amino acids such as glycine and alanine Etc. may be added.

The kinds of the cosmetics of the present invention are not particularly limited, but include solid soap, liquid soap, facial cleansing foam, facial cleansing milk, cleansing gel, cleansing oil,
Cleaning cosmetics such as shampoo and dry shampoo,

Hair rinses, hair conditioners, hair liquids, hair tonics, styling gels, styling mousses, agents for permanent wave, hair dyes, hair cosmetics such as hair nail polish, color rinse,

Vanishing cream, moisture cream, emollient cream, massage cream, whitening cream, anti-wrinkle cream, hand cream,
Basic cosmetics such as emulsions, lotions, beauty essences, skin lotions, and packs,

Makeup cosmetics such as liquid foundation, cream foundation, powder foundation, face powder, compact face powder, cheek, compact cheek, compact eye shadow, etc.,

Sunscreen / sunscreen cosmetics such as sunscreen cream, sunscreen, sun oil, etc.

Nail cosmetics such as base coat, top coat, nail enamel, and nail hard,

Eyeliner, liquid mascara, eyeliner cosmetics such as solid mascara,

Lip cosmetics such as lipsticks and lip balms,

Oral cosmetics such as toothpaste and mouthwash,

Further, cosmetics such as bathing cosmetics such as bath salts and bath oils can be mentioned.

The cosmetic product of the present invention has an EP
L, an organic acid salt, a surfactant, an organic acid, a pH buffer solution, and other cosmetic raw materials may be blended to prepare a cosmetic product. Alternatively, a preservative containing EPL, an organic acid salt, an organic acid, and a pH buffer may be prepared in advance, and other cosmetic raw materials and the preservative may be appropriately mixed to obtain a cosmetic.

Next, the cosmetic preservative, which is another invention of the present invention, will be described in detail. The EPL used in the cosmetic preservative of the present invention may be obtained by any method, but the EPL used in the cosmetic of the present invention can be similarly used.

In the cosmetic preservative of the present invention, the EPL content is not particularly limited, but it is preferably in the range of 0.0001 to 40% by weight based on the cosmetic preservative.

The cosmetic preservative of the present invention contains an organic acid salt in addition to EPL. The organic acid salt used in the cosmetic preservative of the present invention is not particularly limited, but among the organic acid salts, citrate, malate, lactate, acetate, succinate, and phosphate are Can be preferably used in the present invention.

The content of the organic acid salt in the cosmetic preservative of the present invention is not particularly limited, but is preferably 0.0001 to 60% by weight based on the cosmetic preservative.

It is preferable to further add an organic acid to the cosmetic preservative of the present invention. The organic acid used in the cosmetic preservative of the present invention is not particularly limited, but among the organic acids, citric acid, malic acid, lactic acid, acetic acid, succinic acid, and phosphoric acid can be preferably used.

The content ratio of the organic acid in the cosmetic preservative of the present invention is not particularly limited, but is preferably 0.0001 to 60% by weight based on the cosmetic preservative.

The cosmetic preservative of the present invention preferably contains a pH buffer solution. PH used in the present invention
The buffer solution is not particularly limited, but a weak acidic substance-
Any pH buffer solution can be used as long as it is a pH buffer solution composed of a weakly basic substance, a weakly acidic substance-strongly basic substance, and a strongly acidic substance-weakly basic substance. Especially citric acid,
A pH buffer solution containing one or more kinds of acids selected from malic acid, lactic acid, acetic acid, succinic acid, and phosphoric acid and salts thereof (citric acid-citrate buffer solution, malic acid-malate buffer solution, (Lactic acid-lactate buffer, acetic acid-acetate buffer, etc.)
However, it can be preferably used in the present invention.

In the present invention, a pH buffer solution adjusted in advance may be added to the cosmetic preservative of the present invention. Also,
To the cosmetic preservative of the present invention, an organic acid salt contained in the cosmetic preservative and an aqueous organic acid solution capable of forming a buffer solution are added at an appropriate ratio to form a pH buffer solution in the cosmetic preservative. Good.

The content ratio of the pH buffer solution contained in the cosmetic preservative of the present invention is not particularly limited, but may be in the range of 0.01 to 99.9% by weight based on the cosmetic preservative. preferable.

Furthermore, the cosmetic preservative of the present invention may contain a surfactant and / or an amino acid having a microbial growth inhibitory effect, as long as the effects of the present invention are not impaired.

The content ratio of the surfactant and / or amino acid in the cosmetic preservative of the present invention is preferably in the range of 0.0001 to 20% by weight based on the cosmetic preservative.

Examples of the surfactant include monoesters of glycerin and fatty acids having 8 to 12 carbon atoms, monoesters and diesters of polyglycerin having 2 to 9 degree of polymerization and fatty acids having 8 to 16 carbon atoms, and sucrose. Examples thereof include monoesters and diesters of fatty acids having 8 to 16 carbon atoms, and etherification products of sugars and fatty acids having 8 to 16 carbon atoms. These surfactants are excellent in safety and effect of inhibiting microbial growth and can be preferably used in the cosmetic preservative of the present invention.

Examples of the amino acid include amino acids such as glycine and alanine. These amino acids are excellent in safety and microbial growth inhibitory effect and can be preferably used in the cosmetic preservative of the present invention.

In the cosmetic preservative of the present invention, as long as the effect and safety of the present invention are not impaired, bamboo extract, allyl mustard oil, protamine, tea extract, grapefruit seed extract, lysozyme, chitosan. , And naturally occurring antibacterial substances such as hinokitiol may be added.

The cosmetic preservative of the present invention may be in any form such as liquid, paste, powder, flakes and pellets.

The method for producing the cosmetic preservative of the present invention in liquid or paste form is not particularly limited, but water,
EPL, an organic acid salt, an organic acid, a surfactant and / or an amino acid are added to a suitable solvent such as a hydrous alcoholic solution or a hydrous glycerin solution in the range of the above content ratio, respectively, and then stirred and homogenized. It can be obtained by

The method for producing the powdery cosmetic preservative of the present invention is not particularly limited, but powdery EPL, powdery organic acid salt, powdery organic acid, powdery surfactant and / or Alternatively, it can be obtained by adding powdered amino acids in the respective ranges of the above content ratios, and then mixing and homogenizing.

If necessary, sugars such as erythritol, trehalose, starch and cellulose, acid value magnesium, titanium oxide, talc, diatomaceous earth, etc. may be used as long as the effects and safety of the present invention are not impaired. Inorganic powder, synthetic resin powder, etc. may be added to the cosmetic preservative of the present invention as a filler.

The method of producing the cosmetic preservative of the present invention in the form of flakes or pellets is not particularly limited, but the cosmetic preservative of the present invention in powder form is subjected to a pressure molding method, a trommel molding method, and free-flow granulation. It can be obtained by molding by a known method such as a method or a vibration granulation method.

The cosmetic preservative of the present invention exhibits an excellent growth inhibitory effect against a wide range of microorganisms such as bacteria, yeasts and molds. Therefore, if the cosmetic preservative is used in cosmetics, it is possible to obtain a cosmetic that is less susceptible to spoilage and deterioration by microorganisms.

The content ratio of the cosmetic preservative in the cosmetic containing the cosmetic preservative of the present invention is not particularly limited, but 0.001 to 5% by weight relative to the cosmetic.
It is preferable that the range is.

The cosmetic preservative of the present invention is used for various cleaning cosmetics, hair cosmetics, basic cosmetics, makeup cosmetics, suntan / sunscreen cosmetics, nail cosmetics, eyeliner cosmetics, lip cosmetics, oral cosmetics, and baths described above. It can be suitably used as a preservative for cosmetics.

[0081]

EXAMPLES The present invention will be described in detail below with reference to examples. In addition, "%" in the following examples is "% by weight" unless otherwise specified. In this example, ε-polylysine (EPL) is 25% of ε-polylysine manufactured by Chisso Corporation.
An aqueous solution was used. In the comparative example, paraben is methyl p-hydroxybenzoate manufactured by Wako Pure Chemical Industries, Ltd. (hereinafter,
Described as "methylparaben". ) Was used.

1. Lotion 1 1-1. Preparation of lotion 1 (Examples 1 to 4, Comparative examples 1 to 1)
3) In accordance with the proportions shown in Table 1, sterile purified water, EPL, methylparaben, organic acid salts (trisodium citrate, disodium malate, sodium lactate), organic acids (citric acid), nonionic surfactants (pyrolysis) Glutamic acid isostearic acid polyoxyethylene glycer, polyoxyethylene hydrogenated castor oil), anionic substance (sodium hyaluronate), and other ingredients (1,3 butylene glycol, glycerin, propylene glycol) are mixed and then heated to 80 ° C. The temperature was raised, dissolved, and cooled to room temperature with stirring to obtain lotions (Examples 1 to 4 and Comparative Examples 1 to 3).

[0083]

[Table 1]

1-2. Stability test 1 The stability test of the prepared lotion was carried out by the following method. 30 each of the lotions of Examples 1 to 4 and Comparative Examples 1 to 3
The cosmetic was left to stand in a thermostat at 90 ° C. for 90 days and visually observed for changes with time of each cosmetic. The results are shown in Table 2. Also,
The evaluation criteria were two levels: ◯: Hue change, precipitation, and suspended matter were not observed, and X: Hue change, precipitate, and suspended matter were observed.

[0085]

[Table 2]

As is clear from Table 2, the lotions of Examples 1 to 4 of the present invention were compared with the lotions of Comparative Examples 1 to 3,
It can be seen that they have equivalent stability.

1-3. Preservation test 1 The preservative test of the prepared lotion was carried out by the following method. Each of the lotions of Examples 1 to 4 and Comparative Examples 1 to 3 was inoculated with the test bacterium and then allowed to stand in a thermostat at 25 ° C. On the 1st, 3rd, 7th, 14th, 21st, 28th day after standing, the viable cell count in 1 g (1 mL) of each lotion was measured by the standard agar medium method and detected. The number of days until the viable cell count becomes 10 cells / g or less was used as an index of the antiseptic effect. The results are shown in Table 3. The types of test bacteria and the method of preparation are as follows.

Test bacterium: Candida albicans
dida albicans (ATCC 1023
1)) Preparation method: Using a potato dextrose broth medium, the test bacteria precultured at 25 ° C. for 48 hours were suspended in sterile physiological saline at a concentration of 10 ⁶ cells / mL to obtain a test bacterial solution. It was 10 g of lotion (10
0.1 mL was inoculated to and mixed with each other.

Test bacterium: Aspergillus niger (Asp
ergillus niger (ATCC 160
4)) Preparation method: Using potato dextrose agar, 2
The spores of the test bacterium that had been cultivated at 5 ° C for 14 days were treated with polysorbate.
10 in sterile saline with 0.05% 80% ⁶Individual/
The suspension was adjusted to a concentration of mL to obtain a test bacterial solution. Got
The test bacterial solution was applied to 10 g (10 mL) of lotion
0.1 mL was inoculated and mixed.

Test bacterium: Escherichia coli
(richia coli (ATCC 8739)) Preparation method: Test bacterium pre-incubated for 24 hours at 30 ° C. in normal broth medium was added to sterile physiological saline at 10 ⁷ cells / m 2.
The suspension was adjusted to a concentration of L to obtain a test bacterial solution. The test bacterial solution thus obtained was diluted with 0.
1 mL was inoculated and mixed.

Test bacterium: Pseudomonas aeruginosa (A
TCC 9027)) Preparation method: Test bacterium pre-incubated at 30 ° C. for 24 hours in normal broth medium was added to sterile physiological saline at 10 ⁷ cells / mL.
A suspension of the test strain was obtained to obtain a test bacterial solution. The obtained test bacterial solution was added to 0.1 g per 10 g (10 mL) of lotion.
mL was inoculated and mixed.

Test bacterium: Staphylococcus aureus (ATC
C 6538)) Preparation method: Test bacterium pre-incubated at 30 ° C. for 24 hours in a normal broth medium was added to 10 ⁷ cells / mL in sterile physiological saline.
A suspension of the test strain was obtained to obtain a test bacterial solution. The obtained test bacterial solution was added to 0.1 g per 10 g (10 mL) of lotion.
mL was inoculated and mixed.

[0093]

[Table 3] C. albicans: Candida albicans
albicans (ATCC 10231)) A.niger: Aspergillus niger
lus niger (ATCC 1604)) E.coli: Escherichia coli
coli (ATCC 8739)) P. aeruginosa: Pseudomonas aeruginosa (Pse)
udomonas aeruginosa (ATCC
9027)) S. aureus: Staphylococcus aureus (Stap)
hylococus aureus (ATCC 653
8))

As is clear from Table 3, the lotions of Examples 1 to 4 of the present invention were compared with the lotions of Comparative Examples 1 and 2,
It can be seen that even if the nonionic surfactant and the anionic substance are blended, the antiseptic effect is high against all the test bacteria used in the preservative test.

1-4. Skin irritation test 1 Each lotion of Examples 1 to 4 and Comparative Examples 1 to 3 was applied to the inner side of the lower arm of 20 panelists who were sensitive to irritation on sensitive skin, and the irritation felt was self-reported. The results are shown in Table 4. Also, the evaluation criteria are:
±: Slightly stimulating, +; Clearly stimulating, +
+: There are four levels of feeling very stimulating.

[0096]

[Table 4]

As is clear from Table 4, the lotions of Examples 1 to 4 of the present invention have lower skin irritation than the lotions of Comparative Example 2.

2. Emulsion 1 2-1. Preparation of Emulsion 1 (Examples 5 to 8 and Comparative Examples 4 to 6) Sterile purified water, EPL, methylparaben, organic acid salts (trisodium citrate, disodium malate, sodium lactate) according to the ratios shown in Table 5. , Organic acid (citric acid), phospholipids (hydrogenated soybean phospholipids), polysaccharide-forming polysaccharides (xanthan gum), and other components (behenyl alcohol, liquid paraffin, 1,3 butylene glycol, squalane), and then 80 ° C Emulsion (Example 5
8 and Comparative Examples 4 to 6) were obtained.

[0099]

[Table 5]

2-2. Stability test 2 For each emulsion of Examples 9-12 and Comparative Examples 7-9,
The stability test was conducted according to the method described in the above-mentioned “1-2. Stability test 1”. The results are shown in Table 6. Also,
The evaluation criteria were two levels: ◯: Hue change, precipitation, and suspended matter were not observed, and X: Hue change, precipitate, and suspended matter were observed.

[0101]

[Table 6]

As is clear from Table 6, the emulsions of Examples 5 to 8 of the present invention have the same or higher stability than the emulsions of Comparative Examples 4 to 6.

2-3. Preservation test 2 With respect to each emulsion of Examples 5 to 8 and Comparative examples 4 to 6, a preservability test was conducted according to the method described in the above-mentioned "1-3. Preservation test 1". The results are shown in Table 7.

[0104]

[Table 7] C. albicans: Candida albicans
albicans (ATCC 10231)) A.niger: Aspergillus niger
lus niger (ATCC 1604)) E.coli: Escherichia coli
coli (ATCC 8739)) P. aeruginosa: Pseudomonas aeruginosa (Pse)
udomonas aeruginosa (ATCC
9027)) S. aureus: Staphylococcus aureus (Stap)
hylococus aureus (ATCC 653
8))

As is clear from Table 7, the emulsions of Examples 5 to 8 of the present invention were compared with the emulsions of Comparative Examples 4 to 6 even if the phospholipid and the viscosity-creating polysaccharide were blended. It can be seen that the antiseptic effect is high against all the test bacteria used in the sex test.

2-4. Skin irritation test 2 With respect to each emulsion of Examples 5 to 8 and Comparative examples 4 to 6, a skin irritation test was performed according to the method described in the above-mentioned "1-4. Skin irritation test 1". The results are shown in Table 8.
The evaluation criteria were four levels: −; no irritation, ±; slightly irritation, +: clearly irritation, and ++: very irritation.

[0107]

[Table 8]

As is clear from Table 8, the emulsions of Examples 5 to 8 of the present invention have lower skin irritation than the emulsion of Comparative Example 5.

3. Cream 1 3-1. Cream Preparation 1 (Examples 9 to 12 and Comparative Examples 7 to 9) Sterile purified water, EPL, methylparaben, organic acid salts (trisodium citrate, disodium malate, sodium lactate) according to the ratios shown in Table 9. , Organic acid (citric acid), nonionic surfactant (polyoxyethylene (40) glycol monostearate), and other ingredients (stearic acid, cetanol, purified lanolin, isopropyl myristate, triethanolamine, sorbitol) After mixing, the mixture was heated to 80 ° C., dissolved, and cooled to room temperature with stirring to obtain creams (Examples 9 to 12 and Comparative Examples 7 to 9).

[0110]

[Table 9]

3-2. Stability test 3 With respect to each cream of Examples 9 to 12 and Comparative examples 7 to 9, a stability test was performed according to the method described in the above-mentioned "1-2. Stability test 1". The results are shown in Table 10.
The evaluation criteria were two levels: ◯: Hue change and water separation were not observed, and X: Hue change and water separation were observed.

[0112]

[Table 10]

As is clear from Table 10, the creams of Examples 9 to 12 of the present invention have the same stability as the creams of Comparative Examples 7 to 9.

3-3. Storability test 3 With respect to each cream of Examples 9 to 12 and Comparative examples 7 to 9, a storability test was conducted according to the method described in the above-mentioned "1-3. Storability test 1". The results are shown in Table 11.

[0115]

[Table 11] C. albicans: Candida albicans
albicans (ATCC 10231)) A.niger: Aspergillus niger
lus niger (ATCC 1604)) E.coli: Escherichia coli
coli (ATCC 8739)) P. aeruginosa: Pseudomonas aeruginosa (Pse)
udomonas aeruginosa (ATCC
9027)) S. aureus: Staphylococcus aureus (Stap)
hylococus aureus (ATCC 653
8))

As is clear from Table 11, the creams of Examples 9 to 12 of the present invention were compared with the creams of Comparative Examples 7 to 9 even if the nonionic surfactant was blended, and the storability test was performed. It can be seen that the antiseptic effect is high against all the test bacteria used in.

3-4. Skin irritation test 3 With respect to each cream of Examples 9 to 12 and Comparative Examples 7 to 9, a skin irritation test was performed according to the method described in the above-mentioned "1-4. Skin irritation test 1". The results are shown in Table 12. Also, the evaluation criteria are:-; no irritation is felt, ±;
Slightly stimulating, +; Clearly stimulating, ++;
There are four levels of feeling very stimulating.

[0118]

[Table 12]

As is clear from Table 12, the creams of Examples 9 to 12 of the present invention have lower skin irritation than the cream of Comparative Example 8.

4. Shampoo 1 4-1. Shampoo Preparation 1 (Examples 13 to 16 and Comparative Examples 10 to 12) Sterile purified water, EPL, methylparaben, organic acid salts (trisodium citrate, disodium malate, sodium lactate) according to the ratios shown in Table 13. , Organic acids (citric acid), anionic surfactants (triethanolamine lauryl sulfate, sodium polyoxyethylene lauryl ether sulfate-3 ethylene oxide adduct), nonionic surfactants (coconut oil fatty acid diethanolamide), and others After mixing the components (propylene glycol), the temperature was raised to 80 ° C., dissolved, and cooled to room temperature with stirring, and shampoo (Examples 13 to
16 and Comparative Examples 10-12) were obtained.

[0121]

[Table 13]

4-2. Stability Test 4 With respect to each shampoo of Examples 13 to 16 and Comparative Examples 10 to 12, a stability test was performed according to the method described in the above-mentioned "1-2. Stability test 1". The results are shown in Table 14. In addition, the evaluation criteria were two levels: ◯: no hue change, precipitation, or suspended matter was observed, ×: hue change, precipitate, or suspended matter was observed.

[0123]

[Table 14]

As is clear from Table 14, the shampoos of Examples 13 to 16 of the present invention have stability equal to or higher than that of the shampoos of Comparative Examples 10 to 12.

4-3. Storability test 4 With respect to each shampoo of Examples 13 to 16 and Comparative Examples 10 to 12, a storability test was conducted according to the method described in the above-mentioned "1-3. Storability test 1". The results are shown in Table 15.

[0126]

[Table 15] C. albicans: Candida albicans
albicans (ATCC 10231)) A.niger: Aspergillus niger
lus niger (ATCC 1604)) E.coli: Escherichia coli
coli (ATCC 8739)) P. aeruginosa: Pseudomonas aeruginosa (Pse)
udomonas aeruginosa (ATCC
9027)) S. aureus: Staphylococcus aureus (Stap)
hylococus aureus (ATCC 653
8))

As is clear from Table 15, the shampoos of Examples 13 to 16 of the present invention have an anionic surfactant and a nonionic surfactant as compared with the shampoos of Comparative Examples 10 to 12. Even, Candida albicans, and Pseudomonas aeruginosa (Pseudomonas)
It can be seen that the antiseptic effect against aeruginosa) is high.

4-4. Skin irritation test 4 For each shampoo of Examples 13 to 16 and Comparative Examples 10 to 12, a skin irritation test was performed according to the method described in the above-mentioned "1-4. Skin irritation test 1". The results are shown in Table 16. The evaluation criteria were four levels: −; no irritation, ±; slightly irritation, +: clearly irritation, and ++: very irritation.

[0129]

[Table 16]

As is clear from Table 16, the shampoos of Examples 13 to 16 of the present invention have lower skin irritation than the shampoo of Comparative Example 11.

5. Cosmetic preservative 5-1. Preparation of cosmetic preservatives (Examples 13 to 18) After mixing the components according to the blending ratios shown in Table 17, the ingredients were uniformly dissolved to prepare the cosmetic preservatives of Examples 13 to 18.

[0132]

[Table 17]

The cosmetics (lotion, milky lotion, cream, shampoo) to which the cosmetic preservatives prepared in Examples 13 to 18 were added were prepared according to the following methods.

6. Lotion 2 6-1. Preparation of lotion 2 (Examples 19 to 24 and Comparative examples 13 to 15) Sterile purified water, cosmetic preservative (cosmetic preservative of Examples 13 to 18, methylparaben), nonionic interface according to the ratios shown in Table 18. Activators (polyoxyethylene glycerate, isostearic acid pyroglutamate, polyoxyethylene hydrogenated castor oil), and other ingredients (1,3 butylene glycol, glycerin, propylene glycol)
After mixing and, the mixture was heated to 80 ° C., dissolved, and cooled to room temperature with stirring to obtain lotions (Examples 19 to 24 and Comparative Examples 13 to 15).

[0135]

[Table 18]

6-2. Stability test 5 With respect to each lotion of Examples 19 to 24 and Comparative examples 13 to 15, a stability test was performed according to the method described in the above-mentioned "1-2. Stability test 1". The results are shown in Table 19. In addition, the evaluation criteria were two levels: ◯: no hue change, precipitation, or suspended matter was observed, ×: hue change, precipitate, or suspended matter was observed.

[0137]

[Table 19]

As is clear from Table 19, the lotions of Examples 19 to 24 prepared by blending the cosmetic preservatives of Examples 13 to 18 of the present invention are compared with the lotions of Comparative Examples 13 to 15. And it turns out that it has the same stability.

6-3. Preservation test 5 With respect to each lotion of Examples 19 to 24 and Comparative examples 13 to 15, a preservability test was conducted according to the method described in the above-mentioned "1-3. Preservation test 1". The results are shown in Table 20.

[0140]

[Table 20] C. albicans: Candida albicans
albicans (ATCC 10231)) A.niger: Aspergillus niger
lus niger (ATCC 1604)) E.coli: Escherichia coli
coli (ATCC 8739)) P. aeruginosa: Pseudomonas aeruginosa (Pse)
udomonas aeruginosa (ATCC
9027)) S. aureus: Staphylococcus aureus (Stap)
hylococus aureus (ATCC 653
8))

As is clear from Table 20, the lotions of Examples 19 to 24 prepared by blending the cosmetic preservatives of Examples 13 to 18 of the present invention are compared with the lotions of Comparative Examples 13 to 15. Then, it can be seen that the antiseptic effect is high against all the test bacteria used in the preservative test even if the nonionic surfactant is blended.

6-4. Skin irritation test 5 With respect to each lotion of Examples 19 to 24 and Comparative Examples 13 to 15, a skin irritation test was performed according to the method described in "1-4. Skin irritation test 1" described above. The results are shown in Table 2.
Shown in 1. Also, the evaluation criteria are:
±: Slightly stimulating, +; Clearly stimulating, +
+: There are four levels of feeling very stimulating.

[0143]

[Table 21]

As is clear from Table 21, the lotions of Examples 19 to 24 prepared by blending the cosmetic preservatives of Examples 13 to 18 of the present invention were compared with the lotions of Comparative Example 14, respectively. It can be seen that the skin irritation is low.

7. Lotion 3 7-1. Preparation of Lotion 3 (Examples 25 to 30 and Comparative Examples 16 to 18) According to the ratios shown in Table 22, sterile purified water, cosmetic preservative (cosmetic preservative of Examples 13 to 18, methylparaben), anionic substance Sodium hyaluronate,
Mix D-Sorbit solution (Niken Kagaku Co., Ltd., Sorpitol S), elastin solution (Koken Co., Ltd., hydrolyzed elastin solution), perilla extract (Ichimaru Fuarucos Co., Fualcorex Shiso HB) and stir , To obtain lotions (Examples 25 to 30 and Comparative Examples 16 to 18).

[0146]

[Table 22]

7-2. Measurement of Turbidity Turbidity of 660 nm was measured for each lotion of Examples 25 to 30 and Comparative Examples 16 to 18. The turbidity was measured by using a turbidimeter (Tokyo Koden Co., Ltd., ANA-148), and using a kaolin turbidity 100 standard solution (Wako Blum Co., Ltd.) 10
A 10-fold standard solution of kaolin turbidity diluted twice was measured as a standard solution. The measurement results are shown in Table 23.

[0148]

[Table 23]

As is clear from Table 23, the lotions of Examples 25 to 30 prepared by blending the cosmetic preservatives of Examples 13 to 18 of the present invention were compared with the lotions of Comparative Example 16, respectively. It can be seen that the transparency is high and the transparency is maintained even in the presence of the anionic substance.

7-3. Stability Test 6 Each lotion of Examples 25 to 30 and Comparative Examples 16 to 18 was left in a thermostat at 30 ° C., and changes in turbidity with time were confirmed. Turbidity was measured according to the method described in "7-2. Turbidity measurement" above. The results are shown in Table 24.

[0151]

[Table 24]

As is clear from Table 24, the lotions of Examples 25 to 30 prepared by blending the cosmetic preservatives of Examples 13 to 18 of the present invention maintained the transparency even after 90 days. And it turns out that it is stable.

7-4. Preservation Test 6 Each lotion of Examples 25 to 30 and Comparative Examples 16 to 18 was inoculated with Enterobacter bacteria, which is one kind of Gram-negative bacteria, at 1.0 × 10 ³ cells / mL. , 30
1g (1m of each lotion after 48 hours, 96 hours, 168 hours, 336 hours, 720 hours)
The viable cell count in L) was measured by the standard agar medium method. The results are shown in Table 25.

[0154]

[Table 25]

As is clear from Table 25, the lotions of Examples 25 to 30 prepared by blending the cosmetic preservatives of Examples 13 to 18 of the present invention were compared with the lotions of Comparative Examples 16 to 18, respectively. Thus, it can be seen that the antiseptic effect against Enterobacter is high, and the antiseptic effect is high even in the presence of an anionic substance such as sodium hyaluronate.

8. Emulsion 2 8-1. Preparation of Emulsion 2 (Examples 31 to 36 and Comparative Examples 19 to 21) Sterile purified water, cosmetic preservatives (cosmetic preservatives of Examples 13 to 18, methylparaben), and viscous polysaccharides according to the ratios shown in Table 26. (Xanthan gum) and other ingredients (behenyl alcohol, decaglycer pentastearate, sodium stearoyl lactylate, liquid paraffin,
After mixing with 1,3 butylene glycol and squalane, the mixture was heated to 80 ° C., dissolved and cooled to room temperature with stirring to obtain emulsions (Examples 31 to 36 and Comparative Examples 19 to 2).
1) was obtained.

[0157]

[Table 26]

8-2. Stability Test 7 With respect to each emulsion of Examples 31 to 36 and Comparative Examples 19 to 21, a stability test was performed according to the method described in the above-mentioned "1-2. Stability Test 1". The results are shown in Table 27. In addition, the evaluation criteria were two levels: ◯: no hue change, precipitation, or suspended matter was observed, ×: hue change, precipitate, or suspended matter was observed.

[0159]

[Table 27]

As is clear from Table 27, the emulsions of Examples 31 to 36 prepared by blending the cosmetic preservatives of Examples 13 to 18 of the present invention were compared with the emulsions of Comparative Examples 19 to 21. It can be seen that it has the same or higher stability.

8-3. Preservation test 7 With respect to each emulsion of Examples 31 to 36 and Comparative Examples 19 to 21, a preservability test was carried out according to the method described in the above-mentioned "1-3. Preservation test 1". The results are shown in Table 28.

[0162]

[Table 28] C. albicans: Candida albicans
albicans (ATCC 10231)) A.niger: Aspergillus niger
lus niger (ATCC 1604)) E.coli: Escherichia coli
coli (ATCC 8739)) P. aeruginosa: Pseudomonas aeruginosa (Pse)
udomonas aeruginosa (ATCC
9027)) S. aureus: Staphylococcus aureus (Stap)
hylococus aureus (ATCC 653
8))

As is clear from Table 28, the emulsions of Examples 31 to 36 prepared by blending the cosmetic preservatives of Examples 13 to 18 of the present invention were compared with the emulsions of Comparative Examples 19 to 21. It can be seen that the antiseptic effect is high against all the test bacteria used in this preservability test even if the slime-forming polysaccharide is blended.

8-4. Skin irritation test 6 Each emulsion of Examples 31 to 36 and Comparative Examples 19 to 21 was subjected to a skin irritation test according to the method described in "1-4. Skin irritation test 1" described above. The results are shown in Table 29.
It was shown to. Also, the evaluation criteria are:
±: Slightly stimulating, +; Clearly stimulating, +
+: There are four levels of feeling very stimulating.

[0165]

[Table 29]

As is clear from Table 29, the emulsions of Examples 31 to 36 prepared by blending the cosmetic preservatives of Examples 13 to 18 of the present invention are more irritating to the skin than the emulsion of Comparative Example 20. It can be seen that the property is low.

9. Cream 2 9-1. Cream Preparation 2 (Examples 37 to 42 and Comparative Examples 22 to 24) Sterile purified water, cosmetic preservatives (cosmetic preservatives of Examples 13 to 18 and methylparaben), and nonionic surfactant according to the ratios shown in Table 30. After mixing the agent (polyoxyethylene (40) glycol monostearate) and other ingredients (stearic acid, cetanol, purified lanolin, isopropyl myristate, triethanolamine, sorbitol, citric acid), raise the temperature to 80 ° C. , Melt, cool to room temperature with stirring and cream (Example 3
7-42 and Comparative Examples 22-24) were obtained.

[0168]

[Table 30]

9-2. Stability Test 8 With respect to each cream of Examples 37 to 42 and Comparative Examples 22 to 24, a stability test was conducted according to the method described in the above-mentioned “1-2. Stability Test 1”. The results are shown in Table 31. The evaluation criteria were two levels: ◯: Hue change and water separation were not observed, and X: Hue change and water separation were observed.

[0170]

[Table 31]

As is clear from Table 31, the creams of Examples 37-42 prepared by blending the cosmetic preservatives of Examples 13-18 of the present invention were compared with the creams of Comparative Examples 22-24. It can be seen that they have equivalent stability.

9-3. Storability test 8 For each of the creams of Examples 37 to 42 and Comparative Examples 22 to 24, a storability test was conducted according to the method described in the above-mentioned "1-3. Storability test 1". The results are shown in Table 32.

[0173]

[Table 32] C. albicans: Candida albicans
albicans (ATCC 10231)) A.niger: Aspergillus niger
lus niger (ATCC 1604)) E.coli: Escherichia coli
coli (ATCC 8739)) P. aeruginosa: Pseudomonas aeruginosa (Pse)
udomonas aeruginosa (ATCC
9027)) S. aureus: Staphylococcus aureus (Stap)
hylococus aureus (ATCC 653
8))

As is clear from Table 32, the creams of Examples 37 to 42 prepared by blending the cosmetic preservatives of Examples 13 to 18 of the present invention were compared with the creams of Comparative Examples 22 to 24, respectively. It can be seen that, even if a nonionic surfactant is blended, the antiseptic effect is high against all the test bacteria used in this preservability test.

9-4. Skin irritation test 7 A skin irritation test was performed on each cream prepared in Examples 37 to 42 and Comparative Examples 22 to 24 according to the method described in the above-mentioned "1-4. Skin irritation test 1". It was The results are shown in Table 33. The evaluation criteria were four levels: −; no irritation, ±; slightly irritation, +: clearly irritation, and ++: very irritation.

[0176]

[Table 33]

As is clear from Table 33, the creams of Examples 37 to 42 prepared by blending the cosmetic preservatives of Examples 13 to 18 of the present invention were compared with the cream of Comparative Example 23 in comparison with the skin. It can be seen that the irritation is low.

10. Shampoo 2 10-1. Preparation of shampoo 2 (Examples 37 to 42 and Comparative Examples 22 to 24) Sterile purified water, cosmetic preservatives (cosmetic preservatives of Examples 13 to 18 and methylparaben), and anionic surfactant according to the ratios shown in Table 34. Agent (triethanolamine lauryl sulfate, sodium polyoxyethylene lauryl ether sulfate / 3 ethylene oxide adduct), nonionic surfactant (coconut oil fatty acid diethanolamide), and other ingredients (propylene glycol, citric acid, trisodium citrate) ) And then heated to 80 ° C. and cooled to room temperature while emulsifying and mixing to obtain shampoos (Examples 37 to 42 and Comparative Examples 22 to 24).

[0179]

[Table 34]

10-2. Stability Test 9 With respect to each shampoo of Examples 37 to 42 and Comparative Examples 22 to 24, a stability test was performed according to the method described in the above-mentioned “1-2. Stability Test 1”. The results are shown in Table 35. In addition, the evaluation criteria were two levels: ◯: no hue change, precipitation, or suspended matter was observed, ×: hue change, precipitate, or suspended matter was observed.

[0181]

[Table 35]

As is clear from Table 35, the shampoos of Examples 37-42 prepared by blending the cosmetic preservatives of Examples 13-18 of the present invention are the shampoos prepared in Comparative Examples 22-24. It can be seen that it has stability equal to or higher than that of

10-3. Storability test 9 With respect to each shampoo produced in Examples 37 to 42 and Comparative Examples 22 to 24, a storability test was conducted according to the method described in the above-mentioned "1-3. Storability test 1". The results are shown in Table 36.

[0184]

[Table 36] C. albicans: Candida albicans
albicans (ATCC 10231)) A.niger: Aspergillus niger
lus niger (ATCC 1604)) E.coli: Escherichia coli
coli (ATCC 8739)) P. aeruginosa: Pseudomonas aeruginosa (Pse)
udomonas aeruginosa (ATCC
9027)) S. aureus: Staphylococcus aureus (Stap)
hylococus aureus (ATCC 653
8))

As is clear from Table 36, the shampoos of Examples 37-42 prepared by blending the cosmetic preservatives of Examples 13-18 of the present invention were compared with the shampoos of Comparative Examples 22-24. , Candida albicans, and Pseudomonas aeruginosa (Pseudomona), even in the presence of anionic and nonionic surfactants.
It can be seen that the antiseptic effect against S. aeruginosa) is high.

10-4. Skin irritation test 8 For each shampoo prepared in Examples 37 to 42 and Comparative Examples 22 to 24, a skin irritation test was performed according to the method described in the above "1-4. Skin irritation test 1". It was The results are shown in Table 37. Also, the evaluation criteria are
−; No irritation, ±; slightly irritation, +: obviously irritation, ++; very irritation, four levels.

[0187]

[Table 37]

As is clear from Table 37, the cosmetic preservatives of Examples 13 to 18 of the present invention were prepared by mixing the cosmetic preservatives of Examples 13 to 18 with each other. It can be seen that the shampoo of No. 42 has lower skin irritation than the shampoo of Comparative Example 17.

[0189]

The cosmetics and cosmetic preservatives of the present invention are
It is free from adsorption, salt formation, and antibacterial property inhibition by cosmetic ingredients, and the cosmetics are less rough and irritating to the skin. Therefore, the cosmetics and cosmetic preservatives of the present invention include cleaning cosmetics, hair cosmetics, basic cosmetics, makeup cosmetics, suntan / sunscreen cosmetics, nail cosmetics, eyeliner cosmetics, lip cosmetics, oral cosmetics, bath cosmetics, and the like. It can be widely and suitably used for cosmetic applications.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 7/075 A61K 7/075 F term (reference) 4C083 AA03 AA122 AB011 AB281 AC022 AC072 AC092 AC112 AC122 AC132 AC231 AC242 AC271 AC291 AC301 AC302 AC352 AC391 AC402 AC421 AC422 AC432 AC441 AC542 AC581 AC582 AC642 AC782 AD191 AD221 AD332 AD352 AD411 AD412 AD512 AD572 BB01 BB04 BB05 BB07 BB42 BB44 CC04 CC05 CC38 DD23 DD27 DD31 EE01 EE03 EE06 EE10 FF05

Claims (17)

[Claims] 1. A cosmetic containing ε-polylysine and / or a salt thereof and an organic acid salt. 2. The cosmetic according to claim 1, wherein the organic acid salt is at least one selected from citrate, malate, lactate, acetate, succinate, and phosphate. 3. Further, 1 selected from a nonionic surfactant, an amphoteric surfactant, and an anionic surfactant.
The cosmetic product according to claim 1, containing one or more kinds. 4. The method according to claim 1, further comprising an organic acid.
The cosmetic according to any one of 1. 5. The cosmetic according to claim 4, wherein the organic acid is at least one selected from citric acid, malic acid, lactic acid, acetic acid, succinic acid, and phosphoric acid. 6. The cosmetic product according to claim 1, which contains a pH buffer solution. 7. The pH buffer solution is a pH buffer solution containing one or more acids selected from citrate, citric acid, malic acid, lactic acid, acetic acid, succinic acid, and phosphoric acid, and salts thereof. Cosmetics according to 6. 8. A cosmetic preservative containing ε-polylysine and / or a salt thereof and an organic acid salt. 9. The cosmetic preservative according to claim 8, wherein the organic acid salt is at least one selected from citrate, malate, lactate, acetate, succinate, and phosphate. 10. The cosmetic preservative according to claim 8 or 9, further comprising an organic acid. 11. The organic acid is citric acid, malic acid, lactic acid,
The cosmetic preservative according to claim 10, which is one or more selected from acetic acid, succinic acid, and phosphoric acid. 12. The method according to claim 8, which contains a pH buffer solution.
The cosmetic preservative according to any one of 1. 13. The pH buffer is citrate, citric acid,
The cosmetic preservative according to claim 12, which is a pH buffer solution comprising at least one acid selected from malic acid, lactic acid, acetic acid, succinic acid, and phosphoric acid, and a salt thereof. 14. The cosmetic preservative according to claim 8, further comprising a surfactant and / or an amino acid. 15. The surfactant is glycerin and 8 carbon atoms.
To a fatty acid having 12 to 12 carbon atoms, a polyester having a degree of polymerization of 2 to 9 and a fatty acid having 8 to 16 carbon atoms, a monoester and a diester having sucrose and a fatty acid having 8 to 16 carbon atoms, and 15. The cosmetic preservative according to claim 14, which is one or more selected from an etherified product of sugar and a fatty acid having 8 to 16 carbon atoms. 16. The cosmetic preservative according to claim 14, wherein the amino acid is one or more selected from glycine and alanine. 17. A cosmetic containing the cosmetic preservative according to any one of claims 8 to 16.