JP2003033172A5 - Method for introducing a sample into an oocyte or an egg cell - Google Patents
Method for introducing a sample into an oocyte or an egg cell Download PDFInfo
- Publication number
- JP2003033172A5 JP2003033172A5 JP2001263124A JP2001263124A JP2003033172A5 JP 2003033172 A5 JP2003033172 A5 JP 2003033172A5 JP 2001263124 A JP2001263124 A JP 2001263124A JP 2001263124 A JP2001263124 A JP 2001263124A JP 2003033172 A5 JP2003033172 A5 JP 2003033172A5
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- Prior art keywords
- sample
- introducing
- oocyte
- introduction
- egg
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Description
【０００１】
【発明の属する技術分野】
本発明は卵母細胞または卵細胞を選別する装置及び方法、ならびに該選別された細胞に試料を導入する方法に関する。また、特定の膜電位を有する卵母細胞または卵細胞集団、及びこれらを販売または譲渡する方法に関する。
[0001]
Field of the Invention
The present invention relates to an apparatus and method for sorting oocytes or egg cells , and a method for introducing a sample into the sorted cells . It also relates to oocytes or egg cell populations with specific membrane potentials, and methods of selling or transferring them.
さらに上記の従来技術では、選別した細胞の情報を保存することへの配慮がされておらず、選別基準の情報とその後の細胞反応との相関を得ることが困難であった。従って本発明の目的はこれらの相関を得ることにある。また、従来は卵母細胞を手作業で選別していたため、大量生産は不可能であった。従って本発明の他の目的は、一定の基準で選別したことを保証した卵母細胞または卵細胞を、生産・販売及び輸送すること、ならびに該細胞に試料を導入することにある。 Furthermore, in the above-mentioned prior art, no consideration is given to storing the information of the selected cells, and it is difficult to obtain the correlation between the information of the selection criteria and the subsequent cell reaction. The object of the present invention is therefore to obtain these correlations. Also, conventionally, since the oocytes were manually sorted, mass production was impossible. Therefore, another object of the present invention is to produce, sell and transport oocytes or eggs that have been assured to be sorted according to a certain standard , and to introduce a sample into the cells .
【０００５】
【課題を解決するための手段】
本発明は上記課題を解決するために、卵母細胞または卵細胞を選別するための計測手段と、計測結果に基づいて前記卵母細胞または卵細胞を選別する選別手段を有する卵母細胞または卵細胞の選別装置及び方法、ならびに該選別された細胞に試料を導入する方法を提供するものである。
[0005]
[Means for Solving the Problems]
In order to solve the above problems, the present invention is directed to sorting of oocytes or eggs having a sorting means for sorting oocytes or eggs, and sorting said oocytes or eggs based on the measurement results. Provided are an apparatus and method , and a method for introducing a sample into the sorted cells .
Claims (5)
前記卵母細胞または卵細胞を静止膜電位の計測結果に基づいて選別する工程と、 Sorting the oocytes or egg cells based on the measurement result of resting membrane potential;
最上面開口部の最大径が両生類卵母細胞の直径の１０５−１５０％である複数の穴を有するトレイに、前記選別された複数の卵母細胞または卵細胞を移す工程と、 Transferring the plurality of sorted oocytes or egg cells to a tray having a plurality of holes, the largest diameter of the top opening being 105-150% of the diameter of the amphibian oocyte;
前記穴上の前記卵母細胞または卵細胞に、試料を導入する導入針を有する装置を用い、上記トレイに対する上記導入針の相対的位置を移動させながら、前記選別された複数の卵母細胞または卵細胞の各々に、実質的に同じ導入深度で、試料を導入する工程とを有することを特徴とする試料導入方法。 Using a device having an introduction needle for introducing a sample to the oocyte or egg cell on the hole, the plurality of sorted oocytes or egg cells are moved while moving the relative position of the introduction needle to the tray And D. introducing the sample at substantially the same introduction depth to each.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001263124A JP3578341B2 (en) | 2001-05-18 | 2001-08-31 | Sample introduction method to oocyte or egg cell |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001149957 | 2001-05-18 | ||
| JP2001263124A JP3578341B2 (en) | 2001-05-18 | 2001-08-31 | Sample introduction method to oocyte or egg cell |
| JP2001-149957 | 2002-05-18 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001230081A Division JP3519707B2 (en) | 2001-05-18 | 2001-07-30 | Oocyte or egg cell sorting method and apparatus |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2003033172A JP2003033172A (en) | 2003-02-04 |
| JP3578341B2 JP3578341B2 (en) | 2004-10-20 |
| JP2003033172A5 true JP2003033172A5 (en) | 2005-04-14 |
Family
ID=26615361
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001263124A Expired - Fee Related JP3578341B2 (en) | 2001-05-18 | 2001-08-31 | Sample introduction method to oocyte or egg cell |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3578341B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4618674B2 (en) * | 2005-01-17 | 2011-01-26 | セイコーインスツル株式会社 | Invasion confirmation device |
| JP5710135B2 (en) * | 2010-03-18 | 2015-04-30 | 章博 挾間 | Microinsemination device, probe for microinsemination, and microinsemination method |
-
2001
- 2001-08-31 JP JP2001263124A patent/JP3578341B2/en not_active Expired - Fee Related
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