JP2003000227A - New microorganism and organic waste water treating apparatus using the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a new microorganism enabling the treatment of an organic substance in a highly concentrated organic waste water even at a high temperature and provide an organic waste water treating apparatus to use the new microorganism. SOLUTION: The new microorganism is Kluyveromyces sp. Y-1 (FERM BP-7507) or Geotrichum capitatum Y-2 (FERM BP-7508). The organic waste water treating apparatus is provided with a raw water introducing means to introduce the organic waste water, a reaction tank to contact the organic waste water introduced by the introducing means with Kluyveromyces sp. Y-1 (FERM BP-7507) or Geotrichum capitatum Y-2 (FERM BP-7508), and a diffusing means to diffuse the content in the reaction tank.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a novel microorganism and an organic wastewater treatment apparatus using the same, and particularly to an oil refinery,
Side dish factory, fried confectionery factory, cake manufacturing factory, ham and sausage manufacturing factory, canning factory, seafood processing factory, cafeteria
The present invention relates to a treatment device for aerobic biological treatment of liquid high-concentration organic wastewater discharged from food-related establishments such as restaurants by using a novel microorganism.

[0002]

2. Description of the Related Art A wastewater treatment method for treating organic matter in wastewater using microorganisms has been known. An activated sludge method is known as an example of such a treatment method. In this method, microorganisms are propagated in wastewater under aerobic conditions, and activated sludge formed by agglomeration of the microorganisms adsorbs organic matter in the wastewater. In order to promote the oxidative decomposition of organic matter by microorganisms and the propagation of microorganisms, a simple mechanism of blowing air into the wastewater and stirring it is adopted.

However, for example, wastewater generated from a food processing factory or a food service industry contains an extremely high concentration of organic matter, and the conventional general activated sludge method has an excessively high organic matter load (BOD load) against microorganisms, which must be dealt with. Various treatment methods have been proposed because it may not be possible (Japanese Patent Publication No. 5).
No. 8-8313, Japanese Patent Publication No. 57-12436,
Japanese Patent Publication No. 56-52636).

As a result, it was possible to reduce the organic matter even if the organic matter was present in the wastewater at a high concentration.

However, when the wastewater containing a large amount of biologically oxidatively degradable organic substances (eg, lipids) is treated with activated sludge, the bacteria that appear there are enzymatic activities that decompose and remove lipids, especially saturated fatty acids. Because of its weakness, the adsorption of lipids is superior, the lipids are adsorbed around the flocs of activated sludge to form a film, and oxygen is not transferred into the flocs, resulting in oxygen deficiency, which inhibits the decomposition and removal of lipids. Will end up. In addition, the lipid is mixed with water in the waste water to form an emulsion,
Either it is present in colloidal form or it is turned into an oil ball, but if the physicochemical treatment to remove only oil is not separately performed, the untreated oil will contaminate the treatment facility. At the same time, the oil content was mixed with the treated water and was discharged untreated. Therefore, when there is a high concentration of biologically oxidatively decomposable organic matter in wastewater, it is necessary to respond to inflow load fluctuations, ensure proper operation management, secure treatment performance, and dispose of excessive sludge that occurs in large quantities. Was necessary and could not be solved simply by the maintenance measures. Therefore, in recent years, as a high-concentration organic wastewater treatment, an aerobic treatment using microorganisms such as yeast (Japanese Patent Laid-Open No. 2000-246284) and an anaerobic treatment using anaerobic bacteria (UASB) (Japanese Patent Laid-Open No. 9-1).
No. 179 gazette) appeared and was adopted.

Here, a schematic structure of the biological organic wastewater treatment apparatus disclosed in Japanese Patent Laid-Open No. 2000-246284 will be briefly described. As shown in FIG. 6, the wastewater containing lipid passes through the screen 41 as inflow water to remove solids (contaminants) of a size not suitable for yeast treatment, and then sent to the flow rate adjusting tank 42. . A predetermined amount of waste water is supplied from the flow rate adjusting tank 42 into the reaction tank 43 as a biological reaction tank. A sensor (not shown) for measuring the pH in the tank is attached to the reaction tank 43, and the pH in the tank is set to the optimum pH 3 to 7 of the lipid-assimilating yeast according to the measurement value of the pH sensor. To this end, an acidic chemical such as sulfuric acid is added. Further, in the reaction tank 43, a pressure levitation means 44 described later is provided.
A return path and a pump (not shown) for recycling the yeast to be used again in the wastewater treatment among the yeasts solid-liquid separated in (3) are provided between the pressure floating means 44. The wastewater (mixed liquid) that has been subjected to the lipolysis treatment by yeast for a certain retention time in the reaction tank 43 is pressurized floating means 44.
Sent to and undergo solid-liquid separation. The treated water (liquid) obtained by solid-liquid separation is sent to the treated water tank 45, a part of the yeast sludge (solid content) is returned to the reaction tank 43 as described above, and the rest is excess yeast to the sludge treatment system. Sent. Treated water tank 4
The treated water in 5 can be subjected to general wastewater treatment such as activated sludge treatment as a post-treatment according to the water quality standard of the discharge destination.

On the other hand, Japanese Unexamined Patent Publication No. 9-1179 discloses an upflow anaerobic sludge bed treatment device (UASB) which has good contact efficiency and a large load by solubilizing the solid content contained in the organic wastewater. ) Is disclosed, and a method for anaerobic digestion of high-concentration organic wastewater and a treatment apparatus therefor are disclosed.

[0008]

Since the conventional wastewater treatment apparatus and method using microorganisms are constructed as described above, they have the following problems to be solved.

In biological water treatment using aerobic microorganisms such as yeast, it is possible to oxidize and decompose a high concentration of organic matter contained in wastewater to some extent, but the activity of aerobic microorganisms is maintained and the treatment is stabilized. In order to carry out, it is desirable to keep the inflowing wastewater and the water temperature of the biological reaction tank at an intermediate temperature (approximately 10 to 30 ° C.).

However, due to factors such as seasonal (summer), geographical (subtropical, tropical) factors, production process, etc., the temperature of wastewater and biological reaction tanks becomes high (about 35 ° C. or higher), and aerobic microorganisms are generated. There has been a problem that the activity is reduced and biological water treatment is hindered, resulting in deterioration of treated water quality. Further, in the biological reaction tank, heat generation due to the biodegradation reaction of the organic matter contained in the wastewater at a high concentration is added, and there is also a problem of causing a vicious cycle in which the water temperature further rises. In particular, when the wastewater contains a large amount of fats and oils that are not easily biologically oxidized and decomposed, the fats and oils are discharged untreated, which causes environmental pollution at the discharge destination.

Therefore, in order to obtain good treated water quality, it is necessary to maintain the activity of aerobic microorganisms, and an expensive temperature control (cooling) device or the like is introduced to lower the water temperature in the biological reaction tank. Although it must be done, the introduction of a cooling device not only complicates operation management, but also causes a problem that construction cost and treatment (operation) cost increase.

Further, the conventional anaerobic treatment using UASB has the following problems to be solved.

Since granules in which anaerobic bacteria and methane bacteria are mixed are formed and the treatment is usually performed at 30 ° C. or higher,
Although the increase in water temperature has almost no effect on biological water treatment, it is difficult to sufficiently decompose and assimilate organic wastewater containing a high concentration of fats and oils that are biologically difficult to oxidize and decompose. Therefore, the processing performance is lowered and the stability is poor.

Since a long residence time is required to sufficiently treat high-concentration organic wastewater by anaerobic treatment, the treatment equipment becomes large, construction cost and operating cost increase, and operation and maintenance are complicated. Become.

Further, even if the anaerobic treated water has been sufficiently treated, if it is directly discharged to a river or the like, there is a great possibility that the environment of the discharge destination is affected, and therefore it is necessary to separately perform aerobic treatment. .

Therefore, a search for a novel microorganism which solves the above-mentioned problems and enables stable and direct microbial treatment of high-concentration organic wastewater (especially one containing a large amount of fats and oils) even at high water temperature, and further the novel microorganism There is a need to provide an organic wastewater treatment device that utilizes

The present invention has been made to solve the above problems, and an object thereof is to provide a novel microorganism capable of oxidatively decomposing organic matter even at high temperatures. Also, by introducing a high concentration of organic wastewater into a biological reaction tank using such a novel microorganism, an organic wastewater treatment that enables aerobic stable and efficient biological treatment even at high water temperature The purpose is to provide a device. Furthermore, even when an organic wastewater containing a large amount of fats and oils is introduced, since it is treated at a high water temperature, the fats and oils remain liquid and do not solidify directly, enabling aerobic stable and efficient biological treatment. An object is to provide an organic wastewater treatment device.

[0018]

The present invention provides a novel Kluyveromyces species Y-1 and Geotricum capitatum Y-2 capable of oxidatively decomposing organic matter even at high water temperature in high-concentration organic wastewater treatment. provide. The present invention also provides an organic wastewater treatment apparatus using these novel microorganisms.

1. Searching means First, the novel Kluyveromyces species Y-1 and Geotricum capitatum Y- according to the present invention.
The second search means will be described.

Palm oil mill wastewater (Palm) containing palm oil, which is one of vegetable oils and fats, as high-concentration organic wastewater.
Below Oil Mill Effluent, POME
Prepared). Next, this POME was put into a bioreactor and acclimated at 50 ° C. under aerobic conditions. Then, a predetermined amount (100 ml) of the mixed solution was sampled from the biological reaction tank, and then directly with a platinum loop, a standard agar medium (NA), yeast malt extract agar medium (YMA), potato dextrose agar medium (PDA), and bromocresol. Streaked on purple (BCP) agar medium, 46, 48, 5 respectively
Static culture was carried out at 0 ° C. for 3 to 5 days. The formed colony was further subcultured twice under the same conditions. Next, this colony was examined for its ability to assimilate oils and fats (assuming that the strain forming the colony has an ability to assimilate oils and fats). That is, the cells were directly streaked on a BCP agar plate medium containing 0.5% crude palm oil as the sole carbon source, and further statically cultured at 46 ° C to 50 ° C for 3 to 5 days. After culturing, the colonies formed on the agar plate medium were collected, and finally the two yeast strains could be separated. These yeast strains were designated as Y-1 and Y-2, respectively, and used for the examination of mycological properties described later.

The composition of the standard agar medium (NA) is as follows: yeast extract: 2.5 g, peptone: 5.0 g, glucose: 1.0 g, agar: 15.0 g (in 1 L of medium, p.
H7.0). The yeast malt extract agar medium (YMA) had a composition of yeast extract: 3.0 g, peptone: 5.0 g, glucose: 10.0 g, malt extract: 3.0 g, and agar: 15.0 g. Potato dextrose agar (PDA) is potato leachate: 2
00.0g, glucose: 20.0g, agar: 15.0
g (pH 5.6 ± 0.2 in 1 L of medium). BCP
The composition of the agar medium is crude palm oil: 5.0 g, peptone:
0.5 g, yeast extract: 1.0 g, BCP (bromocresol purple): 0.06 g, adecanol TS-9
10: 0.1 g, agar: 15.0 g (in 1 L of medium; pH
7.0). As the medium used for the identification test, the medium to which BTB was added for the sugar fermentability test is: sugar to be tested: 2% yeast extract: 4.5 g, peptone: 7.5
g (in 1 L of medium; pH 7.0), OF medium (manufactured by Eiken Chemical Co., Ltd.) and the like were used. As the medium used in the lipase production test, the Kluyveromyces medium is soytone: 20.0 g, peptone: 30.0 g, yeast:
3.0 g, malt extract: 3.0 g, glucose: 10.
_{0g, NaNO 3: 1.0g, KH} 2 PO 4: 1.0
_{g, MgSO 4 · 7H 2 O} : 0.5g ( culture medium 1L; p
H6.0), the medium for the genus Geotricum is olive oil: 1
%, Peptone: 50.0 g, yeast: 3.0 g, malt extract: 3.0 g, glucose: 10.0 g, NaNO
₃ : 1.0 g, KH ₂ PO ₄ : 1.0 g, MgSO ₄ ·
7H ₂ O: 0.5 g (in 1 L of medium; pH 6.0). The culture can be carried out under aerobic conditions, and basically, liquid culture or solid culture may be used.

As will be described below, the results of the examination of the mycological properties revealed that they were novel strains of the genus Kluyveromyces and the genus Geotricum, respectively, and the above Y-1 and Y-2 will be described later. Kluyveromyces Species Y-1 and Geotricum Capitatum Y
-2. Next, Kluyveromyces species Y-1 and Geotricum capitatum Y-2 thus obtained will be described in detail in terms of properties of novel microorganisms.

2. Mycological properties Table 1 summarizes the mycological properties of the strains newly obtained in the present invention.

[0024]

[Table 1]

A. Kluyveromyces Species Y-
1 (1) Morphological properties Colony morphology: round shape,
Uniform, smooth and shiny. Larger than 1 mm in diameter, dot-like, white in color. As regards cell characteristics, ascospore formation ability: positive, hyphae formation ability: negative.

(2) Growth temperature Growth temperature: less than 55 ° C

(3) Physiological properties In the fermentation test, glucose: positive, galactose:
Positive, Saccharose: Negative, Maltose: Negative, Lactose: Negative, Raffinose: Negative, Nitrate assimilation: Negative, Ester odor: Negative, Glucose utilization: Positive, Glycerin utilization: Positive, 2-Keto D-gluconate calcium Availability: negative, L-arabinose Availability: negative, D
-Xylose utilization: negative, Adnit utilization: positive,
Xylitol utilization: negative, galactose utilization: positive, inositol utilization: negative, D-sorbitol utilization: positive, α-methyl-D-glucoside utilization: negative,
N-acetyl-D-glucosamine utilization: positive, D-cellobiose utilization: negative, lactose utilization: negative, maltose utilization: negative, saccharose utilization: negative, D
-Trehalose utilization: negative, D-meletitose utilization: negative, D-raffinose utilization: negative, lipase:
negative.

Since the above-mentioned mycological and biochemical properties, especially the growth temperature are higher than those of ordinary yeasts, and ascospores are formed, the microorganism Y-1 of the present invention belongs to the genus Kluyveromyces. Was identified as a new yeast strain belonging to (Kluyveromyces), and it was recognized that it was suitable to belong to Kluyveromyces sp. In the present specification, this new yeast strain is referred to as Kluyveromyces species Y-1.

B. Geotricum capitatum Y-2 (1) Morphological properties Regarding colony characteristics, colony morphology: round,
The surroundings are uneven, uneven and slightly glossy. Diameter 1m
The dots are larger than m and the color tone is cream to white. Ascospore formation ability: negative, hyphae formation ability: positive.

(2) Growth temperature Growth temperature: less than 55 ° C

(3) Physiological properties In the fermentation test, glucose: negative, galactose:
Negative, Sucrose: Negative, Maltose: Negative, Lactose: Negative, Raffinose: Negative, Nitrate assimilation: Negative, Ester odor: Negative, Glucose utilization: Positive, Glycerin utilization: Positive, 2-Keto D-gluconate calcium Availability: negative, L-arabinose Availability: negative, D
-Xylose utilization: negative, Adnit utilization: negative,
Xylitol utilization: negative, galactose utilization: positive, inositol utilization: negative, D-sorbitol utilization: negative, α-methyl-D-glucoside utilization: negative,
N-acetyl-D-glucosamine utilization: negative, D-cellobiose utilization: negative, lactose utilization: negative, maltose utilization: negative, saccharose utilization: negative, D
-Trehalose utilization: negative, D-meletitose utilization: negative, D-raffinose utilization: negative, lipase:
negative.

The microorganism Y-2 of the present invention belongs to the genus Geotrichum due to the above-mentioned mycological and biochemical properties, particularly higher growth temperature than in the case of ordinary yeast, and by forming hyphae. Identified as a novel yeast strain, Geotricum species
chum sp. ) Was deemed appropriate. In the present specification, this new yeast strain is referred to as Geotricum capitatum Y-2.

In identifying the above-mentioned yeast strain, Kluyveromyces species Y-1 and Geotricum.
The lipase activity of Capitatum Y-2 was measured.

Kluyveromyces species Y-1 and Geotricum capitatum Y-2 grow well in any general nutrient medium well known to those skilled in the art. Any carbon source may be used as long as the bacterium can assimilate. As the nitrogen source, organic nitrogen sources such as peptone, yeast extract, malt extract, and meat extract can be used. The pH of the medium is adjusted to 2.0 to 12.0, preferably 4.0 to 9.0, more preferably 5.0 to 8.0, and sterilized before use.

The mutant strain obtained by mutating the yeast strain naturally or by artificial means such as gene recombination, radiation treatment, chemical treatment, etc., as long as it has high temperature resistance, the present invention. Shall be included in.

3. Deposit of Microorganisms This bacterium was deposited at the Institute of Biotechnology, National Institute of Advanced Industrial Science and Technology, Ministry of Economy, Trade and Industry on March 15, 2001, and the deposit number is Kluyveromyces sp.
M BP-7507, Geotricum Capitatum Y
-2 is FERMBP-7508.

Next, the organic wastewater treatment apparatus according to the present invention uses a novel high temperature resistant aerobic microorganism.

It is generally said that thermophilic aerobic microorganisms have an extremely high internal respiration rate as compared with mesophilic microorganisms (normal temperature bacteria). For thermophilic microorganisms, there is a higher continuous energy requirement and, on the other hand, there is a higher frequency of microbiological decline. Due to these facts, in the high temperature treatment system, the amount of surplus sludge generated due to biological water treatment is reduced as compared with the medium temperature treatment system, which leads to a reduction in sludge disposal cost.

By the way, it is said that the water temperature in the biological reaction tank is high.
Sprouting is the supply of oxygen to aerobic microorganisms in the reaction mixture.
Is expected to be difficult. Generally, oxygen in water
The saturation concentration decreases as the water temperature increases (fresh water
When the water temperature is 25 ° C, the oxygen saturation concentration becomes 8.3 mg / L.
On the other hand, when the temperature is 60 ° C, the oxygen saturation concentration is 4.6 mg /
L). Therefore, pure oxygen gas is used for air diffusion into the biological reaction tank.
It is possible that However, the oxygen diffusion
The number increases with increasing water temperature (at 25 ° C,
2.5x10^-5cm^Two/ Sec. On the other hand, at 60 ℃
In case of 6.1x10 ^-5cm^Two/ Sec). Therefore, high water temperature
Oxygen transfer rate below is equivalent to that under medium water temperature
Or it will be higher than that. Therefore,
As the oxygen supply means, the dissolved oxygen amount (DO) in the mixed liquid
If it can be secured to a certain extent, even with air aeration, pure oxygen gas can be used.
Even aeration will be fine. When pure oxygen is used, oxygen
It is necessary to fully consider the amount used.

The high water temperature in the biological reaction tank means that many known mesophilic pathogenic microorganisms have been killed or that substances harmful to living organisms are exposed to high temperatures. However, its toxicity may be weakened or detoxified, and it is expected that it is not necessary to sterilize treated water with chlorine as in the conventional case.

Furthermore, when the water temperature in the biological reaction tank is high, the viscosity of the reaction tank mixed liquid is lowered, and the sludge settling property is improved,
Improvement of solid-liquid separation can be expected. As a result, the amount of microorganisms in the reaction tank can be kept high (higher MLSS), so that the reaction tank can be operated with a reduced organic matter load (BOD load), and organic matter can be decomposed and removed efficiently and stably. . In other words, the treatment of organic wastewater with high temperature resistant aerobic microorganisms is more efficient than the conventional treatment of aerobic microorganisms at a medium temperature such as standard activated sludge method. It can be understood that there are more advantages in such as.

The Kluyveromyces species Y-1 and the Geotricum capitatum Y-2 of the present invention are described below in order to specifically explain in what point the novel microorganisms of the present invention are useful. The organic wastewater treatment apparatus used will be described as an example.

[0043]

BEST MODE FOR CARRYING OUT THE INVENTION An organic wastewater treatment apparatus using a novel microorganism based on the present invention will be described.

First, the basic structure of the organic wastewater treatment apparatus using the novel microorganism according to the present invention will be briefly described.

FIG. 1 is for explaining a basic configuration example of an organic wastewater treatment apparatus according to the present invention, and (a) to (f) are block diagrams showing different configuration examples. In FIG. 1, 2 is a screen for removing foreign matters contained in the inflow water (a foreign matter removing device), 3 is a flow rate adjusting tank for alleviating fluctuations in the flow of the inflow water, and the inflow water is internally provided. Means are provided to provide aeration or mechanical agitation to prevent settling separation. Reference numeral 4a is an extrusion flow type biological reaction tank (yeast reaction tank), and only floating sludge (aeration tank), floating carrier loading (carrier charging aeration tank), or installation of contact filter medium (contact oxidation tank) ) Can be selected. By introducing the floating type carrier or installing the contact filter medium, it becomes possible to reduce the concentration of the suspended sludge while securing the amount of sludge in the biological reaction tank 4a. Separation becomes good, and eventually the treated water can be improved. 4b is a batch-type biological reaction tank (yeast reaction tank), and by making the biological reaction tank 4b a batch type, it becomes possible to eliminate or reduce the flow rate adjustment. Further, in the biological reaction tank 4b, the aeration can be stopped and the mixed solution can be allowed to settle to perform solid-liquid separation, thus eliminating the need for a solid-liquid separation device. 5 is a solid component (sludge)
It is a solid-liquid separation device for separating water and liquid components (treated water), and is generally called a settling tank. Reference numeral 10 is a pretreatment facility installed for the purpose of removing suspended substances such as contaminants in the inflow water and facilitating biological water treatment in the latter stage. Specifically, a gravity sedimentation tank, a pressure levitation device. , A centrifuge, a filtration device, etc. are used alone or in combination.

The organic wastewater treatment apparatus of the present invention decomposes organic substances contained in high-concentration organic wastewater aerobically and stably by the action of microorganisms contained in the biological reaction tanks 4a or 4b. Is. Therefore, biological reaction tank 4
The state of a or 4b is desired to be as follows.

That is, the temperature of the water in the biological reaction tank 4a or 4b should not exceed 60 ° C., preferably 35 to 5
The organic substance decomposition reaction is performed at 5 ° C.

The pH in the biological reaction tank 4a or 4b is generally 5 to 8, and the organic matter (BOD) concentration of the organic wastewater introduced into the biological reaction tank 4a or 4b is 5,000 to 3.
50,000 mg / L (0.5-3.0%), especially 1
Even if a high-concentration organic wastewater of 50,000 mg / L or more is introduced, it can be treated efficiently.

In addition, when treating with high temperature resistant oil and fat assimilating bacteria together with a novel microorganism, even if the concentration of the hexane extractable substance in the organic wastewater is 500 mg / L (0.05%) or more, good results are obtained. Can be processed.

The biological reaction tank 4a or 4b is an aeration tank in which the above microorganisms are suspended by the air diffused by the air diffuser, or a carrier-introduced aeration tank in which a flowable carrier carrying the above microorganisms is charged, or It is desirable that the contact aeration tank is provided with a contact filter medium carrying the above microorganisms,
The reaction tank is not limited to this as long as it can aerobically treat the organic wastewater in the presence of the above microorganisms and the like.

The aforesaid organic wastewater may be continuously introduced into the biological reaction tank 4a or 4b to perform aerobic biological treatment in an extrusion flow system, or the organic wastewater may be introduced batchwise. The aerobic biological treatment may be carried out in a batch inflow manner.

In the biological reaction tank 4a or 4b, air is supplied from an aeration means to aerate and agitate the inside of the tank to perform aerobic biological treatment. However, air having an increased oxygen concentration according to the required oxygen amount or the like is used. Pure oxygen may be used. It should be noted that if the amount of oxygen required for microbial treatment is obtained by aeration stirring using pure oxygen, but the stirring strength is not sufficiently obtained,
A stirring device may be used separately.

When the organic wastewater introduced into the bioreaction tank 4a or 4b contains fats and oils, the fats and oils are introduced into the above-mentioned bioreaction tanks 4a and 4b in a liquid state or an emulsion state without solidifying the fats and oils to obtain a high water temperature. Bacillus, a microorganism that has lipase activity even under
sp. ) And Ralstonia
p. ) In one reaction tank 4a or 4b, Kluyveromyces sp. Ys and / or Geotricum
It is preferable to coexist with Capitertum Y-2 (oil and fat decomposing means) and aerobically biologically treat fats and oils. As a result, the concentration of the hexane extract substance in the organic wastewater is 500 mg.
Even if the amount is / L (0.05%) or more, not only organic substances but also oils and fats can be treated well in a single biological reaction tank.

When the organic wastewater introduced into the biological reaction tank 4a or 4b contains oil and fat, a new oil and fat decomposing tank 6 (oil and fat decomposing means) may be separately provided before and after the reaction tank. At that time, the fat or oil is introduced into the fat or oil decomposing tank 6 in a liquid state or an emulsion state without being solidified, and Bacillus sp. Or Ralstonia (Ra) which is a microorganism having a lipase activity even at a high water temperature.
lstonia sp. The microorganisms) and organic wastewater may be contacted to aerobically biologically treat fats and oils.

The fats and oils contained in the organic wastewater are vegetable fats and animal fats, particularly, in the case of vegetable fats and oils, Bacillus sp. And Ralstonia which are microorganisms having a lipase activity even at high water temperature. (Ralstonia sp.) Microorganisms can also be used in combination to efficiently perform aerobic biological treatment even on vegetable oils and fats that are only organic substances.

Next, an example of the organic wastewater treatment apparatus using the novel microorganism according to the present invention will be specifically described.

<Example 1> FIG. 2 is a block diagram for explaining an example of an organic wastewater treatment apparatus using a novel microorganism according to the present invention. Similar to the organic wastewater treatment apparatus disclosed in Japanese Unexamined Patent Publication No. 2000-246284 shown in FIG. 6, except that the novel Kluyveromyces species Y-1 and the novel Geotricum capitatum Y-2 of the present invention are used. Can be configured.

The organic wastewater passes through the screen 2 as inflow water, and after removing solid matters (contaminants) having a size not suitable for biological treatment, it is sent to the flow rate adjusting tank 3.
A predetermined amount of waste water is supplied from the flow rate adjusting tank 3 into the biological reaction tank (yeast reaction tank) 4. A sensor (not shown) for measuring the pH in the biological reaction tank 4 is attached to the biological reaction tank 4, and the pH in the tank is adjusted to a value (generally pH 5 to 5) suitable for the yeast according to the measured value of the pH sensor. In order to achieve 8), a pH adjusting agent such as sulfuric acid may be added. After performing aerobic treatment with yeast for a certain retention time in the biological reaction tank 4, the mixed liquid in the reaction tank is sent to a solid-liquid separation device 5 such as a pressure floating means to undergo solid-liquid separation. . The treated water (liquid) obtained by solid-liquid separation is sent to the treated water tank 14, and part of the separated sludge (solid content) is returned sludge to the biological reaction tank 4
And the rest is sent to the sludge treatment system as excess sludge. The treated water in the treated water tank 14 can be subjected to general wastewater treatment such as activated sludge treatment or advanced treatment as a post-treatment according to the water quality standard of the discharge destination.

Tables 2 and 3 show examples of the operating conditions of the organic wastewater treatment apparatus and the treatment results in the intermediate temperature treatment or the high temperature treatment relating to the removal of the organic matters from the high-concentration organic wastewater containing fats and oils by yeast.

[0060]

[Table 2]

[0061]

[Table 3]

As shown in Table 2, focusing on the treatment performance of the T-BOD and the hexane (Hex) extraction substance, in the case of the medium temperature treatment (25 to 30 ° C.), the influent T-BOD was 3%.
The treated water T-BOD concentration was 813 mg / L with respect to 2,594 mg / L, and the treated water hexane extractable substance concentration was 98 mg / L with respect to the inflow water hexane extractable substance concentration of 4,355 mg / L. . On the other hand, high temperature treatment (50-
55 ° C), the influent T-BOD concentration is 38,344
The treated water T-BOD concentration is 3,210 with respect to mg / L.
The result was that the hexane extract substance concentration of the influent water was 6,750 mg / L, whereas the hexane extract substance concentration of the treated water was 90 mg / L. Therefore, by using Kluyveromyces species Y-1 and Geotricum capitatum Y-2 according to the present invention, it is difficult not only to treat the organic matter contained in high-concentration organic wastewater at moderate temperature but also to perform normal biological treatment. It was found that it can be decomposed and removed efficiently even at high temperatures. Moreover, in this system, by using a high-temperature-resistant microorganism having lipase activity in combination (oil and fat decomposing means), it is possible to efficiently decompose and assimilate fats and oils at medium or high temperatures like organic substances.

<Embodiment 2> FIG. 3 is a block diagram for explaining an example of a biological organic wastewater treatment apparatus according to the present invention. Here, two reaction tanks (biological reaction tank 4
The case of biological treatment in the oil and fat decomposition tank 6) will be described. That is, in the oil and fat decomposing tank 6, first, high-temperature-resistant bacteria (Bacillus species B-3 and Ralstonia picetti B) having an oil and fat degrading assimilation ability (lipase activity).
-4) is used to decompose and assimilate the oils and fats contained in the organic wastewater, and the treated water separated by the solid-liquid separation device 15 is favorably used in the biological reaction tank 4 in the latter stage in high load operation using yeast. Temper temper. Here, Bacillus species B-3 and Ralstonia picetti B-4, which are high-temperature-resistant bacteria having an ability to assimilate fats and oils (lipase activity), have deposit numbers: FERM BP-7509 and FERM BP, respectively.
It has been deposited as -7510, and it is very preferable to use it, but it is not limited to this as long as it is a bacterium having similar properties.

The organic wastewater treatment device of this embodiment is a so-called batch type device, and is a screen (contamination remover) for removing contaminants contained in the organic wastewater (raw water) introduced through the flow path 1a. 2, a flow rate adjusting tank 3 that temporarily stores the raw water treated by the foreign matter removing device 2 to adjust the flow rate, and an air supply that supplies air or pure oxygen gas to an air diffuser (not shown) A means 8 is connected, and an oil and fat decomposing tank 6 for oxidatively decomposing oil and fat contained in raw water flowing from the flow rate adjusting tank 3 by high temperature resistant oil and fat assimilating bacteria, and a sludge discharging means 9 flowing out from the oil and fat decomposing tank 6 The solid-liquid separation device 15 for solid-liquid separating the mixed liquid flowing out from the fat and oil decomposition tank 6 and the air supply means 8 for supplying air or pure oxygen gas to the air diffusion means (not shown) are connected and fixed. The separated liquid separated by the liquid separation means 15 (organic waste ) Schematically composed of the bioreactor (yeast reactor) 4 which performs aerobic treatment by high-temperature-resistant aerobic microorganisms by introducing a (yeast).

An embodiment of the organic wastewater treatment apparatus having such a structure will be shown. The POME 1a passes through the screen 2 which is a contaminant removing device as the input raw water to remove the solid content (contaminants) of a size not suitable for biological treatment, and then adjusts the flow rate via the flow path 1b. Transferred to the tank 3, where homogenization is achieved. Next, a certain amount of the homogenized wastewater from the flow rate adjusting tank 3 via the flow path 1c is converted into high temperature resistant oil and fat-utilizing bacteria (that is, Bacillus species B-3).
And / or Ralstonia picetti B-4) or the like is supplied as inflow water into the oil-decomposition tank 6 that holds sludge. Here, as a method of supplying inflow water to the oil and fat decomposition tank 6, there are a method of introducing the inflow water from one place and a method of so-called stepwise inflow to divide the inflow water from several places, and either method may be selected. In the oil / fat decomposition tank 6, a high-concentration organic wastewater treatment is carried out by an aerobic method including aeration of air or pure oxygen using high-temperature-resistant oil / fat-utilizing bacteria.
Therefore, a predetermined amount of air, pure oxygen, or the like is supplied from the air supply means 8 to the oil / fat decomposition tank 6. The treatment liquid obtained by the decomposition of oil and fat by the high temperature resistant oil and fat-utilizing bacteria is solid-liquid separated by the solid-liquid separator 15 via the flow path 1d. The separated liquid (supernatant water) obtained here is further sent to the biological reaction tank 4 via the flow path 1e, while the sludge is returned to the fat and oil decomposition tank 6. Biological reaction tank 4 is Kluyveromyces
The sludge containing the species Y-1 and Geotricum capitatum Y-2 is retained, and the organic matter is decomposed in the separated liquid in the solid-liquid separation tank 15 having a reduced fat content. The separated liquid is aerobically treated by using air or pure oxygen gas sent from the air supply means 8. A part of the separated sludge separated by the solid-liquid separation device 15 is sent as return sludge to the oil and fat decomposition tank 6 via the discharging means 9, and the rest is transferred as excess sludge to a sludge treatment facility (not shown). To be done. If there is an existing anaerobic pond or aerobic pond (oxidation pond) at the final discharge destination, the excess sludge may be directly transferred from the discharge means 9 to the above pond, so that the sludge treatment facility can be omitted. The treated water by the organic wastewater treatment apparatus may be discharged as it is to a public water area, or may be treated by a treatment facility (not shown) at a subsequent stage using biological treatment or physicochemical treatment.

FIG. 4 shows a screen (contaminant removing device) 2 and a flow rate adjusting tank 3 in the organic wastewater treatment device shown in FIG.
It is a block diagram for demonstrating the case where the pressure levitation apparatus 10 (pretreatment equipment) is newly provided between and.

The coagulant is added from the coagulant adding means 11 to the wastewater that has passed through the screen 2 and passed through the flow path 1f, and then the wastewater is stored in the pressure levitation apparatus 10. After storing a predetermined amount, air or a nitrogen-rich gas is injected into the pressure levitation device 10 from the air supply means 12 to bring the pressure levitation device 10 into a pressurized state. The air or nitrogen gas sent from the air supply means 12 is sufficiently brought into contact with and mixed with the waste water, and left for a while. After leaving, the wastewater is at least the floss part,
Divided into 3 layers: intermediate water and sludge deposit (deposit). The floss part is discharged to the oil recovery system (not shown) by the oil discharge means 13, and the sludge accumulation part is discharged by the discharge means 9. The intermediate water from which the froth centering on the oil and sludge is removed is transferred to the flow rate adjusting tank 3 via the flow path 1b and further sent to the oil / fat decomposition tank 6 for oil / fat decomposition treatment.

FIG. 5 is a graph showing the daily change in the removal rate of the hexane extract in POME-treated water. That is,
This is an example of actually treating the organic wastewater using the organic wastewater treatment device as shown in FIG. The hexane extraction substance removal rate is the organic wastewater treatment device based on the present invention,
This is especially for evaluating the processing capacity of oils and fats that are difficult to biodegrade. It should be noted that in the following description, the change with time is represented using the actual date and time.

As input raw water, organic wastewater obtained from POME was subjected to pressure flotation treatment using a pressure flotation device (pretreatment device) 10, and foreign substances removed from wastewater were used as input raw water. did.

First, the wastewater treatment was carried out by the organic wastewater treatment device under the following operating conditions for 4 months.

The operating conditions at this time are shown below. Reaction tank actual capacity:
5L, aeration method: aeration with pure oxygen only, input raw water: POME with impurities removed, input raw water amount: 0.2-0.5L /
d, reaction tank water temperature: 60 ° C., pH in reaction tank: pH 5.0 or less, dissolved oxygen amount (DO): 2.0 mg / L or more, biological oxygen demand (BOD) volume load: 1.1 to 2 .6 kg
/ M 3 ^· d, in addition to the charged raw, as seed sludge, the sludge 50g~100g collected containing microorganisms generated by the contact portion between the pit inner wall and the water surface in the middle wastewater path, a week 1-2
It was charged into the biological reaction tank 4 at a rate of once.

Under such operating conditions, the hexane extractable substance removal rate was about 60% or less. This indicates that the wastewater treatment by the organic wastewater treatment device is not performed efficiently and the decomposition of organic substances including fats and oils is insufficient.

Therefore, only on April 1, the pH in the reaction vessel was raised to pH 5.6 or higher by using an alkaline aqueous solution,
Further, from May 4, when the reactor water temperature was lowered to 60 ° C. or lower and the operation was continued, the hexane extraction substance removal rate gradually increased and finally came to maintain 90% or higher.
In addition, the pH in the reaction tank also tended to increase with the biodegradation of organic substances. Furthermore, after May 15, the input of seed sludge was stopped. The operation results from May 16 to July 15 when the treatment status was stable and favorable are shown below.

Reaction tank actual capacity: 5 L, aeration method: pure oxygen
Aeration with pure water, input raw water: POME without impurities, input raw water
Water amount: 0.45-0.5 L / d, treated water p after completion of reaction
H: pH 6.4 to 9.0 (pH unadjusted), negative BOD volume
Load: 2.0-3.0kg / m ^Three・ D, hexane extract
Removal rate: 96% or more.

As described above, in the conventional treatment equipment, the high concentration of organic wastewater flowing in has a high biochemical oxygen demand (BOD), which makes it difficult to efficiently perform biological treatment.
Further, the solid-liquid separability of the mixed solution is also deteriorated, which hinders stable biological treatment. Therefore, measures such as diluting the organic wastewater were taken, but the problem of diluting the wastewater leading to an increase in the installation area of the treatment equipment and the treatment time and a rise in the running cost, and no longer effective biological When the organic wastewater contains fats and oils that are not easily biodegraded, the fats and oils could not be sufficiently decomposed and treated. On the other hand, the biological treatment apparatus for high-concentration organic wastewater using the novel high-temperature-resistant aerobic microorganisms based on the present example, the organic wastewater and the high-temperature-resistant aerobic microorganisms are contacted to each other under high water temperature. However, organic matter can be efficiently decomposed and removed. Furthermore, if the high-concentration organic wastewater contains fats and oils,
By using a high temperature resistant oil and fat-utilizing bacterium together, the oil and fat can be directly biologically decomposed and removed. When the high temperature resistant aerobic bacteria and the high temperature resistant oil and fat-utilizing bacteria are used together in one reaction tank, the water temperature and dissolved oxygen (DO) in the reaction tank should be adjusted so that good organic matter removal and oil and fat removal can be performed. Management of pH and pH (operation management) is important.

When high-concentration organic wastewater treatment is carried out using Kluyveromyces sp. Ys-1 and Geotricum capitatum Y-2 of the present invention, the high-concentration organic matter is mainly treated by the yeast and the sludge generation amount is increased. Can be suppressed.
Furthermore, when treating highly concentrated organic wastewater containing fats and oils, Bacillus species B having lipase activity is used.
By using -3 (FERM BP-7509) and Ralstonia picetti B-4 (FERM BP-7510), fats and oils can be directly decomposed. Further, since it is a thermotolerant bacterium, it can actively proliferate and metabolize even in a reaction tank in a high temperature state. Therefore, if the temperature in the reaction tank is 35 ° C. or higher, the fats and oils in the waste liquid are present in a liquid or emulsion state, and thus the bacterial lipase and the fats and oils easily form an enzyme-substrate complex, and the fats and oils are It has a low molecular weight to the extent that it can be absorbed by bacterial cells and is used for bacterial cell synthesis and energy generation. Therefore, the load of the organic substance treatment by the yeast in the latter part is reduced by performing such fat and oil decomposition in the former part. This shows that the load is reduced even when the treatment with yeast is performed in the first stage and the fat and oil is decomposed with bacteria in the second stage.

In the organic wastewater treatment tank apparatus shown in FIG. 3 or 4, both Bacillus species B-3 and Ralstonia picetti B-4 were used in the oil and fat decomposing tank 6, but only one of the bacteria was used. It is also possible to use. Similarly, in the biological reaction tank 4, only one of Kluyveromyces species Y-1 and Geotricum capitatum Y-2 may be used.

Further, in the organic wastewater treatment apparatus of FIG. 3 or 4, the two reaction tanks 4 and 6 were used. However, as described above, one or both of the reaction tanks are included in each reaction tank. The coexistence of the above microorganisms makes it possible to treat high-concentration organic wastewater containing fats and oils in a single tank. In this case, in order to allow the microorganisms to coexist in one reaction tank, it is important to manage the water temperature, dissolved oxygen (DO), pH and the like in the reaction tank (operation management).

In the above example, the raw reaction water 4 was subjected to the foreign matter removal treatment with the screen 2 and then the biological reaction tank 4 was used.
However, the present invention is not limited to this, and when the amount of impurities to be removed is small, it is possible to omit the processing of impurities by the screen 2. Alternatively, the organic wastewater may be treated at high temperature by using only air without using pure oxygen, using air and pure oxygen together, or aeration with air having an increased oxygen concentration.

Further, the aerobic biological treatment may be carried out after the water temperature of the input raw water POME is lowered to about ambient temperature.

Further, the treated water obtained by treating the input raw water at an intermediate temperature with the organic wastewater treatment apparatus of the present invention,
It is also possible to further process the activated sludge treatment device in the latter stage to reduce the BOD concentration of the final treated water to 20 mg / L.

Further, a carrier supporting microorganisms is put into the reaction tank, and the PO is aerated by pure oxygen and / or air aeration.
It is also possible to continuously feed ME. For example, as the carrier, rock (eg, pearlite, diatomaceous earth) or a crushed product thereof, gravel, sand, plastics, ceramics (eg, alumina, silica, natural zeolite, synthetic zeolite), talc, etc., particularly porous ceramics, A porous material having continuous vents such as porous plastics is preferable, but it is not limited to this as long as it can flow by aeration in the reaction tank.

When the concentration of contaminants in the treated water cannot be reduced, the contaminants in the treated water can be removed by using the solid-liquid separator 5 and the coagulant feeding means 11 for supplying the coagulant. As the solid-liquid separator 5 that is usually used,
Equipment well known to those skilled in the art, such as vacuum filters, pressure filters,
It is also possible to use a centrifuge or a belt press type dehydrator. As the coagulant, an inorganic coagulant such as ferric chloride or slaked lime or a polymer coagulant is used.

Summarizing what has been described about the examples, the organic wastewater treatment system of the present example has a high concentration of the organic substances by the novel Kluyveromyces spp. Y-1 and the novel Geotricum capitatum Y-2. Bacillus that has a lipase activity and can efficiently and stably aerobically treat biological wastewater even under high water temperature.
Spicies B-3 and Ralstonia Piquetti B-
By using 4 together, it is possible to efficiently and promptly decompose and remove organic substances such as fats and oils contained in high-concentration organic wastewater that are difficult to biologically treat. By treating at a higher temperature, it is possible to improve the solid-liquid separation of highly concentrated mixed liquid and reduce the amount of sludge generated compared to mesophilic biological treatment. In addition, the pressure-floated treated POME can be treated in advance. It is expected that the high-concentration oil contained in the floss obtained by pressure floating will be recovered, and that the precipitate and excess dehydrated sludge obtained from POME will be effectively used for breeding fish and shellfish. it can. In addition, since the batch operation is performed, the reaction tank is used as a solid-liquid separation device (precipitation tank), so there is no need to install a solid-liquid separation device after the reaction tank. Since it has good properties, it is possible to carry out the treatment while keeping the concentration of the mixed liquid in the reaction tank high (10,000 mg / L or more), so it is possible to operate with a reduced load of organic matter (BOD) and to concentrate excess sludge. Since it is not necessary, the sludge thickening tank can be omitted, and the sludge can be directly transferred to the sludge dewatering device in a relatively fresh sludge state after being stored in the sludge storage tank.

As a result, not only the water treatment facility but also the sludge treatment facility can be made compact, and it is possible to realize a treatment device having a small installation area as a whole. Furthermore, since the pH of POME is acidic at around pH 4 originally, a chemical injection facility for adjusting the pH in the reaction tank is also used, and a defoaming agent injection facility is used because foaming that tends to occur in high-concentration organic wastewater hardly occurs. Can be eliminated. Since it is expected that pathogenic microorganisms, viruses, and parasite eggs will be killed by heat, it may not be necessary to install a sodium hypochlorite injection facility, which will reduce running costs such as the cost of chemicals used and also toxicity. It is possible to create a facility that considers the local environment, such as detoxifying substances by heat and not contaminating the discharge destination of treated water. Furthermore, in the organic wastewater treatment apparatus of the present invention, focusing on the properties of lipase produced by a microorganism used in combination for the decomposition of fats and oils, it exhibits resistance (high temperature resistance) even under high water temperature of 50 ° C. or higher, It did not deactivate even at pH 6 or lower. Therefore,
When used in combination with Kluyveromyces sp. Ys and Geotricum capitatum Y-2, which are high-temperature-resistant organic substance-decomposing yeasts of the present invention, Bacillus sp. B-3 and Ralstonia piquetti B-4 The produced lipase acts even more effectively.

[0086]

As described above, according to the present invention, the novel Kluyveromyces species Y-1 and the novel Geotricum capitatum Y-2 are high temperature-resistant microorganisms, and therefore, they are usually treated by biological treatment. It is possible to decompose organic wastewater efficiently even under high water temperature, which is difficult to do.

According to the present invention, the organic wastewater treatment apparatus is a novel Kluyveromyces sp.
By using the species Y-1 and / or the novel Geotricum capitartum Y-2, it becomes possible to efficiently decompose organic substances contained in organic wastewater even under high water temperature where biological treatment is usually difficult. . Furthermore, when the treatment is performed at a high water temperature, the solid-liquid separation property of the mixed liquid is improved, so that the concentration of the mixed liquid in the biological reaction tank can be maintained high,
This makes it possible to reduce the BOD load and perform stable processing.

According to the present invention, in the organic wastewater treatment apparatus, the bioreactor is a novel Kluyveromyces species Y-1 and a novel Geotricum capitatum Y-2.
Aerobic tank filled with a floating carrier carrying at least one of the above, or a new Kluyveromyces
Since it is a catalytic oxidation tank that carries out aerobic treatment with a contact filter medium carrying at least one of species Y-1 and novel Geotricham capitartum Y-2, it is more aerobic because it can stably hold microorganisms. It is possible to decompose organic substances stably and efficiently.

According to the present invention, in the organic wastewater treatment device, the aeration means is an aeration device for supplying air, a gas having an increased oxygen concentration, or pure oxygen to the biological reaction tank,
It becomes possible to decompose the organic substance more efficiently and aerobically.

According to the present invention, the biological organic wastewater treatment apparatus is an aerobic microorganism having lipase activity in a biological reaction tank, preferably Bacillus species B-3.
(FERM BP-7509) and / or Ralstonia picetti B-4 (FERM BP-7510)
By using together (coexisting with), the oil and fat contained in the organic wastewater can be decomposed aerobically and stably and efficiently without directly solidifying at high temperature. In addition, depending on the situation, a separate oil / fat decomposition tank may be provided to separately process the oil / fat in the organic waste water in the presence of the above microorganisms.

[Brief description of drawings]

1A to 1C are views for explaining a basic configuration example of an organic wastewater treatment apparatus according to the present invention.
(F) is a block diagram showing a different configuration example.

FIG. 2 is a block diagram for explaining an example of an organic wastewater treatment device based on the present invention.

FIG. 3 is a block diagram for explaining an example of an organic wastewater treatment apparatus based on the present invention.

FIG. 4 is a view showing a newly added pressure levitation device 1 between the screen 2 and the flow rate adjusting tank 3 in the organic wastewater treatment device shown in FIG.
It is a block diagram for demonstrating the case where 0 is provided.

FIG. 5 is a graph showing the change with time of the hexane extract substance removal rate in POME-treated water.

FIG. 6 is a block diagram for explaining a schematic configuration of an organic wastewater treatment apparatus disclosed in Japanese Patent Laid-Open No. 2000-246284.

[Explanation of symbols]

1a to 1f flow path 2 screens (contamination remover) 3 Flow rate adjustment tank 4,4a, 4b Biological reaction tank (yeast reaction tank) 5,15 Solid-liquid separation device 6 Oil decomposition tank (oil decomposition means) 7 Treated water discharged from organic wastewater treatment equipment 8,12 Air supply means 9 Ejection means 10 Pretreatment equipment (pressurization levitation device) 11 Flocculant charging means 13 Oil discharge means 14 Treated water tank

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) C02F 3/26 C02F 3/26 4D040 3/34 3/34 Z C12M 1/00 C12M 1/00 H 1 / 04 1/04 1/40 1/40 Z // (C12M 1/00 C12R 1: 645 C12R 1: 645) 1:07 (C12M 1/00 C12R 1:01 C12R 1:07) (C12M 1/00 C12R (1:01) (C12N 1/16 C12R 1: 645) (72) Inventor Nobutoshi Nishi 3-6-18 Shibaura, Minato-ku, Tokyo Nishihara Institute of Environmental Health, Ltd. (72) Inventor Tsukasa Shinada Minato-ku, Tokyo Shibaura Third Street No. 6 No. 18 Co., Ltd. Nishihara environment Institutes of health in the F-term (reference) 4B029 AA02 BB02 BB07 CC03 CC10 4B065 AA01X AA15X AA72X AC20 BB02 BB10 BB12 BC06 BC42 CA55 4D003 AA01 AA12 AB01 BA03 CA02 CA03 DA09 DA30 EA19 EA22 EA23 EA24 EA25 EA30 FA04 4D028 AB00 AB03 AC01 BB01 BB02 BC17 BC18 BC24 BC28 BD07 CD01 4D029 AA01 AB05 BB10 CC01 4D040 DD03 DD07 DD12 DD14 DD24

Claims (7)

[Claims] 1. Kluyveromyces sp. Ys-1 (accession number: FERM BP-7) that forms ascospores
507). 2. A mycelium-forming Geotricum capitatum Y-2 (accession number: FERM BP-750).
8). 3. Raw water introducing means for introducing organic wastewater,
A bioreactor for contacting the organic wastewater with Kluyveomyces sp. Ys-1 and / or Geotricum capitatum Y-2, and an aeration means for aerating the inside of the bioreactor The organic wastewater treatment equipment to be. 4. The biological reaction tank is any one of an aeration tank, an aeration tank in which a floating carrier supporting microorganisms is added, and a catalytic oxidation tank in which a contact filter medium supporting microorganisms is installed. The organic wastewater treatment apparatus according to claim 3, which is characterized in that. 5. The aeration means is an aeration device for supplying at least one of air, oxygen-enriched air, and pure oxygen gas into the biological reaction tank. 4. The organic wastewater treatment device according to 4. 6. The organic wastewater according to claim 3, further comprising a fat and oil decomposing means for contacting the organic wastewater with an aerobic microorganism having a lipase activity. Biological processing equipment. 7. The aerobic microorganism having lipase activity is
7. Bacillus species B-3 and / or Ralstonia picetti B-4.
A biological treatment device for the described organic wastewater.