JP2002531138A - 酸化代謝に対する感受性に関して候補化合物をスクリーニングする方法 - Google Patents

酸化代謝に対する感受性に関して候補化合物をスクリーニングする方法

Info

Publication number
JP2002531138A
JP2002531138A JP2000586939A JP2000586939A JP2002531138A JP 2002531138 A JP2002531138 A JP 2002531138A JP 2000586939 A JP2000586939 A JP 2000586939A JP 2000586939 A JP2000586939 A JP 2000586939A JP 2002531138 A JP2002531138 A JP 2002531138A
Authority
JP
Japan
Prior art keywords
enzyme
metabolism
testosterone
dcf
formation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2000586939A
Other languages
English (en)
Japanese (ja)
Other versions
JP2002531138A5 (enExample
Inventor
ディレン・アール・タッカー
クイピン・チェン
Original Assignee
ユニバーシティ オブ ノース カロライナ アット チャペル ヒル
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ユニバーシティ オブ ノース カロライナ アット チャペル ヒル filed Critical ユニバーシティ オブ ノース カロライナ アット チャペル ヒル
Publication of JP2002531138A publication Critical patent/JP2002531138A/ja
Publication of JP2002531138A5 publication Critical patent/JP2002531138A5/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2000586939A 1998-12-04 1999-11-15 酸化代謝に対する感受性に関して候補化合物をスクリーニングする方法 Pending JP2002531138A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/205,762 1998-12-04
US09/205,762 US6312917B1 (en) 1998-12-04 1998-12-04 Method of screening candidate compounds for susceptibility to oxidative metabolism
PCT/US1999/027130 WO2000034506A2 (en) 1998-12-04 1999-11-15 Method of screening candidate compounds for susceptibility to oxidative metabolism

Publications (2)

Publication Number Publication Date
JP2002531138A true JP2002531138A (ja) 2002-09-24
JP2002531138A5 JP2002531138A5 (enExample) 2006-09-14

Family

ID=22763550

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2000586939A Pending JP2002531138A (ja) 1998-12-04 1999-11-15 酸化代謝に対する感受性に関して候補化合物をスクリーニングする方法

Country Status (12)

Country Link
US (2) US6312917B1 (enExample)
EP (1) EP1135521B1 (enExample)
JP (1) JP2002531138A (enExample)
KR (1) KR20010093140A (enExample)
AT (1) ATE317914T1 (enExample)
AU (1) AU774229B2 (enExample)
BR (1) BR9916458A (enExample)
CA (1) CA2352523A1 (enExample)
DE (1) DE69929900T2 (enExample)
MX (1) MXPA01005528A (enExample)
NZ (1) NZ512217A (enExample)
WO (1) WO2000034506A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007531538A (ja) * 2004-04-05 2007-11-08 ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒル 活性酸素種に基づいたチトクロームｐ４５０阻害ハイスループット測定法

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6632630B2 (en) * 2000-03-29 2003-10-14 Aclara Biosciences, Inc. Monooxygenase assays
WO2003102192A1 (en) * 2002-05-30 2003-12-11 Astex Technology Limited Methods of purification of cytochrome p450 proteins and of their crystallizing
US20070179716A1 (en) * 2001-04-02 2007-08-02 Astex Therapeutics Limited Crystal structure of cytochrome P450 3A4 and uses thereof
US20050032119A1 (en) * 2001-04-02 2005-02-10 Astex Technology Ltd. Crystal structure of cytochrome P450
US7148046B2 (en) * 2001-04-02 2006-12-12 Astex Therapeutics Limited Crystal structure of cytochrome P450
US20050159901A1 (en) * 2001-04-02 2005-07-21 Astex Technology Limited Crystal structure of cytochrome P450
JP2005512532A (ja) * 2001-08-03 2005-05-12 ディヴァーサ コーポレイション Ｐ４５０酵素、それらをコードした核酸及びそれらの作製と使用
US20040053383A1 (en) * 2001-10-25 2004-03-18 Astex Technology Ltd. Crystals of cytochrome P450 2C9, structures thereof and their use
DE60214869T2 (de) * 2001-10-25 2007-04-12 Astex Technology Ltd., Cambridge Cytochrome p450 2c9 kristalle, strukturen und dessen verwendung
US20040096874A1 (en) * 2002-04-11 2004-05-20 Third Wave Technologies, Inc. Characterization of CYP 2D6 genotypes
GB0216203D0 (en) * 2002-07-12 2002-08-21 Imp Cancer Res Tech Mondulation of cytochrome P450 reductase activity
EP1546162B1 (en) * 2002-09-20 2011-06-22 Promega Corporation Luminescence-based methods and probes for measuring cytochrome p450 activity
DE03758362T1 (de) * 2002-10-25 2005-08-25 Astex Technology Ltd., Cambridge Kristallstruktur des Cythochroms P450 3A4 und dessen Verwendung
WO2004078131A2 (en) * 2003-03-01 2004-09-16 Symyx Technologies, Inc. Evaluating effects of exposure conditions on drug samples over time
US20060046278A1 (en) * 2004-08-26 2006-03-02 Qualyst, Inc. Methods and kits for determining metabolic stability of compounds
US20060223135A1 (en) * 2005-04-05 2006-10-05 The University Of North Carolina At Chapel Hill High throughput reactive oxygen species-based cytochrome P450 inhibition assay
EP2778234B1 (en) 2005-05-31 2017-09-27 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
US20090318561A1 (en) * 2008-06-23 2009-12-24 Mutual Pharmaceutical Company, Inc. Colchicine products, method of manufacture, and methods of use
WO2010021686A1 (en) 2008-08-18 2010-02-25 Promega Corporation Luminogenic compounds and methods to detect cytochrome p450 3a enzymes
US9790537B2 (en) 2014-01-29 2017-10-17 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
WO2018118897A1 (en) * 2016-12-19 2018-06-28 Dice Molecules Sv, Llc Methods of estimating metabolic stability of tagged combinatorial library compounds
WO2024223797A1 (en) 2023-04-28 2024-10-31 Institut National de la Santé et de la Recherche Médicale Use of cyp3a4 inhibitors for the treatment of hepatitis d virus (hdv) infections

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4341868A (en) * 1978-08-18 1982-07-27 Kyowa Hakko Kogyo Co., Ltd. Method and test composition for the determination of the substrate for xanthine oxidase
DE2965849D1 (en) 1978-08-18 1983-08-18 Kyowa Hakko Kogyo Kk Method and test composition for the determination of a substrate for xanthine-oxidase, including a novel xanthine-oxidase and method for the preparation thereof
JP2515551B2 (ja) * 1987-06-08 1996-07-10 和光純薬工業株式会社 新規な過酸化水素の定量方法
US5413915A (en) 1988-07-12 1995-05-09 Resource Technologies Group, Inc. Method and sensor for detecting toxic chemical exposure effects and metabolic activation of carcinogenic chemical agents
AU1970897A (en) 1996-04-15 1997-11-07 Fox Chase Cancer Center Assays for detection of purine metabolites
GB9821932D0 (en) 1998-10-09 1998-12-02 Univ Leicester Cytochrome P450 electrochemical system

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007531538A (ja) * 2004-04-05 2007-11-08 ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒル 活性酸素種に基づいたチトクロームｐ４５０阻害ハイスループット測定法

Also Published As

Publication number Publication date
NZ512217A (en) 2002-03-28
CA2352523A1 (en) 2000-06-15
DE69929900T2 (de) 2006-08-17
AU1525000A (en) 2000-06-26
EP1135521A2 (en) 2001-09-26
EP1135521B1 (en) 2006-02-15
WO2000034506A3 (en) 2000-11-23
AU774229B2 (en) 2004-06-17
MXPA01005528A (es) 2002-04-24
WO2000034506A2 (en) 2000-06-15
KR20010093140A (ko) 2001-10-27
US6312917B1 (en) 2001-11-06
US20020058295A1 (en) 2002-05-16
DE69929900D1 (de) 2006-04-20
ATE317914T1 (de) 2006-03-15
BR9916458A (pt) 2001-09-04

Similar Documents

Publication Publication Date Title
US6312917B1 (en) Method of screening candidate compounds for susceptibility to oxidative metabolism
Weaver et al. Cytochrome P450 inhibition using recombinant proteins and mass spectrometry/multiple reaction monitoring technology in a cassette incubation
Tang et al. Substrate-dependent effect of acetonitrile on human liver microsomal cytochrome P450 2C9 (CYP2C9) activity
Chauret et al. Description of a 96-well plate assay to measure cytochrome P4503A inhibition in human liver microsomes using a selective fluorescent probe
Feidt et al. Profiling induction of cytochrome p450 enzyme activity by statins using a new liquid chromatography-tandem mass spectrometry cocktail assay in human hepatocytes
Chauret et al. The use of 3-[2-(N, N-diethyl-N-methylammonium) ethyl]-7-methoxy-4-methylcoumarin (AMMC) as a specific CYP2D6 probe in human liver microsomes
Zientek et al. Development of an in vitro drug–drug interaction assay to simultaneously monitor five cytochrome P450 isoforms and performance assessment using drug library compounds
Carr et al. Characterization of the rhesus monkey CYP3A64 enzyme: species comparisons of CYP3A substrate specificity and kinetics using baculovirus-expressed recombinant enzymes
Shou et al. Use of inhibitory monoclonal antibodies to assess the contribution of cytochromes P450 to human drug metabolism
JP2008506395A (ja) 化合物同定用の代謝法
Yamamoto et al. Application of microtiter plate assay to evaluate inhibitory effects of various compounds on nine cytochrome P450 isoforms and to estimate their inhibition patterns
Auld et al. Bioluminescent assays for cytochrome P450 enzymes
Usmani et al. Human cytochrome P450: metabolism of testosterone by CYP3A4 and inhibition by ketoconazole
Venhorst et al. Evaluation of a novel high-throughput assay for cytochrome P450 2D6 using 7-methoxy-4-(aminomethyl)-coumarin
Palamanda et al. Validation of a rapid microtiter plate assay to conduct cytochrome P450 2D6 enzyme inhibition studies
Zambon et al. Evaluation of cytochrome P450 inhibition assays using human liver microsomes by a cassette analysis/LC-MS/MS
WO2002004660A2 (en) Method for testing for inhibition of drug-metabolizing cytochrome p450 isozymes
Nicoll-Griffith et al. Use of a benzyloxy-substituted lactone cyclooxygenase-2 inhibitor as a selective fluorescent probe for CYP3A activity in primary cultured rat and human hepatocytes
Paragas et al. ecoAO: a simple system for the study of human aldehyde oxidases role in drug metabolism
US20230272448A1 (en) An improved substrate cocktail assay for high-throughput screening of cytochrome p450 inhibitors
JP2007531538A (ja) 活性酸素種に基づいたチトクロームｐ４５０阻害ハイスループット測定法
JP3689370B2 (ja) モノオキシゲナーゼアッセイ
US7056654B2 (en) Screening assay for inhibitors of human cytochrome P-450
EP1238097B9 (en) Diethoxyfluorescein as indicator for cytochrome p450
Delaporte et al. Cytochrome P450 assays

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20060727

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20060727

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20090428

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20091006