JP2002519998A - Ｄｎａ中の配列長多型性の検出用の方法および装置 - Google Patents

Ｄｎａ中の配列長多型性の検出用の方法および装置

Info

Publication number
JP2002519998A
JP2002519998A JP2000533592A JP2000533592A JP2002519998A JP 2002519998 A JP2002519998 A JP 2002519998A JP 2000533592 A JP2000533592 A JP 2000533592A JP 2000533592 A JP2000533592 A JP 2000533592A JP 2002519998 A JP2002519998 A JP 2002519998A
Authority
JP
Japan
Prior art keywords
target
repeat units
hybridization
reporter
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2000533592A
Other languages
English (en)
Japanese (ja)
Other versions
JP2002519998A5 (enExample
Inventor
ロナルド・ジー・ソスノウスキ
ユージーン・トゥ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanogen Inc
Original Assignee
Nanogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanogen Inc filed Critical Nanogen Inc
Publication of JP2002519998A publication Critical patent/JP2002519998A/ja
Publication of JP2002519998A5 publication Critical patent/JP2002519998A5/ja
Pending legal-status Critical Current

Links

Classifications

    • H—ELECTRICITY
    • H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
    • H10D—INORGANIC ELECTRIC SEMICONDUCTOR DEVICES
    • H10D64/00—Electrodes of devices having potential barriers
    • H10D64/01—Manufacture or treatment
    • H10D64/021—Manufacture or treatment using multiple gate spacer layers, e.g. bilayered sidewall spacers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00653—Making arrays on substantially continuous surfaces the compounds being bound to electrodes embedded in or on the solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2000533592A 1998-02-25 1999-02-12 Ｄｎａ中の配列長多型性の検出用の方法および装置 Pending JP2002519998A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/030,156 1998-02-25
US09/030,156 US6207373B1 (en) 1998-02-25 1998-02-25 Methods for determining nature of repeat units in DNA
PCT/US1999/003175 WO1999043853A1 (en) 1998-02-25 1999-02-12 Methods and apparatus for determination of length polymorphisms in dna

Publications (2)

Publication Number Publication Date
JP2002519998A true JP2002519998A (ja) 2002-07-09
JP2002519998A5 JP2002519998A5 (enExample) 2006-03-09

Family

ID=21852807

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2000533592A Pending JP2002519998A (ja) 1998-02-25 1999-02-12 Ｄｎａ中の配列長多型性の検出用の方法および装置

Country Status (9)

Country Link
US (3) US6207373B1 (enExample)
EP (1) EP1056887B1 (enExample)
JP (1) JP2002519998A (enExample)
CN (1) CN1252282C (enExample)
AT (1) ATE328114T1 (enExample)
AU (1) AU2765399A (enExample)
CA (1) CA2322206A1 (enExample)
DE (1) DE69931608T2 (enExample)
WO (1) WO1999043853A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005536234A (ja) * 2002-08-19 2005-12-02 ザ プレジデント アンド フェローズ オブ ハーバード カレッジ 進化する新しい分子機能
JP2011507488A (ja) * 2007-10-22 2011-03-10 エルジーシー・リミテッド オリゴヌクレオチドおよびその使用
JP2022514861A (ja) * 2018-12-19 2022-02-16 アイオンコ インコーポレイテッド 変異解析機構及びその操作方法を備えた遺伝情報処理システム

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US6207373B1 (en) * 1998-02-25 2001-03-27 Nanogen, Inc. Methods for determining nature of repeat units in DNA
US6468742B2 (en) 1993-11-01 2002-10-22 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip
US7582421B2 (en) * 1993-11-01 2009-09-01 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
US6379897B1 (en) 2000-11-09 2002-04-30 Nanogen, Inc. Methods for gene expression monitoring on electronic microarrays
US6207673B1 (en) * 1997-03-12 2001-03-27 The University Of North Carolina At Chapel Hill Covalent conjugates of topoisomerase I and topoisomerase II inhibitors
US6013459A (en) 1997-06-12 2000-01-11 Clinical Micro Sensors, Inc. Detection of analytes using reorganization energy
DE69929542T2 (de) 1998-10-27 2006-09-14 Affymetrix, Inc., Santa Clara Komplexitätsmanagement und analyse genomischer dna
WO2000058522A1 (en) * 1999-03-30 2000-10-05 Nanogen, Inc. Single nucleotide polymorphic discrimination by electronic dot blot assay on semiconductor microchips
AU769929B2 (en) * 1999-04-12 2004-02-12 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
US6642046B1 (en) * 1999-12-09 2003-11-04 Motorola, Inc. Method and apparatus for performing biological reactions on a substrate surface
US6824669B1 (en) 2000-02-17 2004-11-30 Motorola, Inc. Protein and peptide sensors using electrical detection methods
US6602400B1 (en) 2000-06-15 2003-08-05 Motorola, Inc. Method for enhanced bio-conjugation events
KR100443569B1 (ko) * 2000-12-15 2004-08-09 학교법인 인하학원 길이다형성 ｄｎａ 염기서열을 이용한 다중 증폭중합효소연쇄반응 조건의 최적화에 의한 개인식별 및친자감별 방법
DE10122659A1 (de) * 2001-05-10 2002-12-05 Infineon Technologies Ag Biochip-Anordnung
US6893822B2 (en) 2001-07-19 2005-05-17 Nanogen Recognomics Gmbh Enzymatic modification of a nucleic acid-synthetic binding unit conjugate
BR0212773A (pt) * 2001-09-24 2004-10-13 One Lambda Métodos para detecção de sequências-alvo de ácido nucléico com marcadores diagnósticos
EP2428570B1 (en) 2001-09-28 2018-05-23 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Microrna molecules
AU2011253686C1 (en) * 2001-09-28 2014-04-03 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Micro-RNA molecules
US20030175947A1 (en) * 2001-11-05 2003-09-18 Liu Robin Hui Enhanced mixing in microfluidic devices
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US20040011650A1 (en) * 2002-07-22 2004-01-22 Frederic Zenhausern Method and apparatus for manipulating polarizable analytes via dielectrophoresis
US7601493B2 (en) * 2002-07-26 2009-10-13 Nanogen, Inc. Methods and apparatus for screening and detecting multiple genetic mutations
US7270952B2 (en) * 2002-09-24 2007-09-18 Intel Corporation Detecting molecular binding by monitoring feedback controlled cantilever deflections
US7125672B2 (en) * 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
EP1687445A4 (en) * 2003-09-23 2007-03-28 Atom Sciences Inc POLYMERIC NUCLEIC ACID HYBRIDIZATION SUBSTANCES
US20060008823A1 (en) * 2004-05-12 2006-01-12 Kemp Jennifer T DNA profiling and SNP detection utilizing microarrays
US9777314B2 (en) * 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
US20070020671A1 (en) * 2005-07-12 2007-01-25 Radtkey Ray R Method for detecting large mutations and duplications using control amplification comparisons to paralogous genes
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US9074244B2 (en) 2008-03-11 2015-07-07 Affymetrix, Inc. Array-based translocation and rearrangement assays
US20130183666A1 (en) 2012-01-18 2013-07-18 Marc N. Feiglin Partial genotyping by differential hybridization
CA2880678A1 (en) * 2012-07-31 2014-02-06 Juan Carlos Jaime A procedure for obtaining and detecting a genetic marker of objects to be identified, the marker, method for authentication and method for verification
AU2021231104B2 (en) * 2020-03-03 2025-02-20 Universiteit Gent Hydrolysis-based probe and method for STR genotyping

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4987071A (en) 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
EP0317239A3 (en) 1987-11-13 1990-01-17 Native Plants Incorporated Method and device for improved restriction fragment length polymorphism analysis
US4963663A (en) 1988-12-23 1990-10-16 University Of Utah Genetic identification employing DNA probes of variable number tandem repeat loci
DE3834636A1 (de) 1988-10-11 1990-04-19 Max Planck Gesellschaft Verfahren zur analyse von laengenpolymorphismen in dna-bereichen
GB8824531D0 (en) 1988-10-20 1988-11-23 Ici Plc Polynucleotide probes
GB2228086A (en) 1988-11-25 1990-08-15 Ici Plc Characterisation of genomic DNA
US5364759B2 (en) 1991-01-31 1999-07-20 Baylor College Medicine Dna typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats
US6051380A (en) * 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US6017696A (en) * 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US5632957A (en) 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
US5849486A (en) * 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
US6048690A (en) * 1991-11-07 2000-04-11 Nanogen, Inc. Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis
US5403708A (en) 1992-07-06 1995-04-04 Brennan; Thomas M. Methods and compositions for determining the sequence of nucleic acids
DE69430665T2 (de) * 1993-01-15 2002-11-21 The Public Health Research Institute Of The City Of New York, Inc. Empfindlicher nukleinsäure-sandwichhybridisierungs-assay und kits
US6207373B1 (en) * 1998-02-25 2001-03-27 Nanogen, Inc. Methods for determining nature of repeat units in DNA
AU2360195A (en) 1994-05-05 1995-11-29 Beckman Instruments, Inc. Oligonucleotide repeat arrays
US5795716A (en) * 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
GB9507238D0 (en) * 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5753439A (en) * 1995-05-19 1998-05-19 Trustees Of Boston University Nucleic acid detection methods
US5908745A (en) 1996-01-16 1999-06-01 University Of Chicago Use of continuous/contiguous stacking hybridization as a diagnostic tool
US6090549A (en) 1996-01-16 2000-07-18 University Of Chicago Use of continuous/contiguous stacking hybridization as a diagnostic tool
AU713667B2 (en) * 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US5881269A (en) 1996-09-30 1999-03-09 International Business Machines Corporation Simulation of multiple local area network clients on a single workstation

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005536234A (ja) * 2002-08-19 2005-12-02 ザ プレジデント アンド フェローズ オブ ハーバード カレッジ 進化する新しい分子機能
JP2010046092A (ja) * 2002-08-19 2010-03-04 President & Fellows Of Harvard College 進化する新しい分子機能
JP2011507488A (ja) * 2007-10-22 2011-03-10 エルジーシー・リミテッド オリゴヌクレオチドおよびその使用
JP2022514861A (ja) * 2018-12-19 2022-02-16 アイオンコ インコーポレイテッド 変異解析機構及びその操作方法を備えた遺伝情報処理システム

Also Published As

Publication number Publication date
WO1999043853A1 (en) 1999-09-02
CN1297489A (zh) 2001-05-30
ATE328114T1 (de) 2006-06-15
EP1056887B1 (en) 2006-05-31
US6395493B1 (en) 2002-05-28
EP1056887A4 (en) 2002-08-28
US6207373B1 (en) 2001-03-27
CA2322206A1 (en) 1999-09-02
WO1999043853A9 (en) 1999-11-11
DE69931608T2 (de) 2007-05-16
US6753148B2 (en) 2004-06-22
AU2765399A (en) 1999-09-15
US20020115098A1 (en) 2002-08-22
EP1056887A1 (en) 2000-12-06
CN1252282C (zh) 2006-04-19
DE69931608D1 (de) 2006-07-06

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