JP2002363561A - Method of manufacturing microbial material - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method of manufacturing a microbial material which comprises as an active ingredient a metabolite of a microorganism that can be cultured in industrially large quantities and which is stable in quality, is easy in preparetion and handling, and can be stored for a long period of time. SOLUTION: The microbial material is manufactured by adding a dilution of a culture solution of a metabolite of a microorganism, for example, Enterobacter cloacae (FERM P-8884), to a powder of an extender (fertilizer, porous matter, etc.), having a particle size of 0.01 mm or less and granulating the resultant mixture by a granulator to obtain granules having a particle size of 0.1 mm-2 mm (90 wt.% or more).

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a method for producing a microbial material. More specifically, the present invention relates to a method for producing a microbial material obtained by processing a metabolite of a microorganism.

[0002]

2. Description of the Related Art Various types of microorganisms inhabit the rhizosphere and root surface of plants, and it is known that the growth of plants depends on these microorganisms. The present applicant has previously revealed that a culture solution using a microorganism exhibits a plant growth promoting effect (Japanese Patent No. 1940249). However, such a useful culture medium of a microorganism has a problem that it is susceptible to other bacteria and is not suitable for long-term storage because a considerable amount of a medium component remains in the culture medium. As a countermeasure for this, it is conceivable to prepare immediately before use and use up all at once, but this is not a drastic solution. Furthermore, when these microbial cultures are sprayed, a considerable amount must be added to the tank at the time of watering, or a diluent must be separately adjusted each time and sprayed on the plant, which is inconvenient to handle. .

As a solution to this problem, the present applicant has previously proposed a freeze-dried product of a microorganism culture solution as a plant growth promoter (Japanese Patent Application Laid-Open No. 2-240008). There is a problem that the possible period is relatively short and it is necessary to dilute when used.

The present applicant has previously proposed a soil conditioner obtained by impregnating a microbial culture solution with porous granules or powder (Japanese Patent Application Laid-Open No. 9-268288). Since the culture solution of the microorganism is freeze-dried in a state of being impregnated in the porous body, it takes time to prepare the granules or powder, and it is difficult to make the diameters of the granules or powder of the material uniform, and the weight is weighed. Alternatively, there is a problem in that it takes time and effort to pack and the effect of promoting the growth of plants varies.

[0005] That is, the above-mentioned publication also states that the particle size of the granules to be used is preferably 0.5 to 2 mm. However, it is difficult to produce granules in this range alone. When a lump is crushed to an arbitrary size and a particle having a particle size other than the intended purpose is obtained, it is necessary to sieve the pulverized material. This is a rather complicated operation, causes a large loss of time, and has a problem that particles having too small a particle size cannot be reused.

[0006] On the other hand, even if commercially available granules are used, the distribution of the particle size is considerably wide, and the work of sieving is still required. Also, in this case, similarly, there has been a problem that particles having a too small particle size cannot be reused.

Further, in this method, after a granule production process (crushing and sieving) is performed to obtain granules having an appropriate particle size,
There is a need for a soil-improving material manufacturing process of impregnating the microorganism culture solution, and two processes are required, such as a granule manufacturing process of an appropriate particle size and a soil-improving material manufacturing process. There were problems such as an increase in the cost of the apparatus.

[0008]

An object of the present invention is to provide a method for producing a microbial material containing a metabolite of a microorganism which can be industrially mass-cultured as an active ingredient. Another object of the present invention is to provide a product which is easy to prepare and handle and can be stored for a long time.

[0009]

SUMMARY OF THE INVENTION The object of the present invention is as follows.
Add microbial metabolites to bulking material powder having a particle size of 0.01 mm or less, granulate with a granulator, and reduce the particle size to 0.1 mm to 2 mm (90% by weight).
This is achieved by the method for producing microbial materials described above.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION The filler powder has a particle size of 0.01
mm or less, preferably 1 to 10 μm is used. If the particle size is larger than that, it becomes difficult to control the particle size, and it takes a long time for granulation. A fertilizer or a porous body is used as the filler. As the porous body, silica, zeolite, or the like is used. Since these porous bodies have a large surface area, a soil improvement material having an increased microbial material content per unit volume can be formed.

The microbial material can be obtained by attaching a metabolite of a microorganism to the surface or the surface and inside of a fertilizer or a porous body. Specifically, metabolites of microorganisms appropriately diluted with water in extender powder, preferably
Add the metabolite of the microorganism diluted 50 to 500 times, more preferably 100 to 250 times, put it in the granulator, rotate it with a stirring blade for about 5 to 15 minutes, and keep the particle size constant about 0.1 to 2 mm It is manufactured by granulating until If it is less than 0.1 mm, it will be easy to fly, while if it is more than 2 mm, the effect will vary.

The granulation is carried out, for example, by using a cylindrical granulator provided with a baffle plate in the depth direction of the jacket portion and having a stirring blade connected to a variable speed motor, and a culture solution charged by the stirring blade. And the stirring blades installed obliquely in the rotation direction wind up silica and the like to the top of the granulator, and only the silica due to the difference in specific gravity between the culture solution and the silica is deposited at the bottom of the granulator. To avoid. The granulator has a jacket for adjusting the culture solution to an arbitrary temperature. This portion is used to prevent the temperature inside the granulator from rising due to stirring. In addition, as the refrigerant, there is no problem as long as it is suitable for cooling the granulator, but usually cheap water is used, the refrigerant entered from the lower part of the jacket, and passed through the inside of the jacket part. After that, it is discharged from above the jacket.

The production of microbial materials is carried out by using such a granulator and appropriately adjusting the number of revolutions of a motor. In other words, the initial rotation speed of the grains is an appropriate number of revolutions
It is preferable to control the particle size by stirring at 1400 to 2000 rpm to make the particle size to some extent and then gradually lowering the rotation. If the number of rotations is higher than this, the particle size grows faster than expected, so that the particle size becomes too large. It will take a long time. The time required by this method is determined depending on the relationship with the number of revolutions, but is about 5 to 15 minutes in all the steps. In practice, at room temperature (15-30 ° C.), 3-5 minutes at 1800 rpm, 2 at 700 rpm
Minutes, 350 rpm for 2 minutes, 1500 rpm for 8 to 12 minutes, 5 minutes
By setting the conditions such as 00 rpm for 3 minutes and 300 rpm for 1 minute, the particle size is 0.1 to 2 mm (95% by weight or more) and 0.5 to 2 mm, respectively.
mm (90% by weight or more) of microbial material can be obtained.

As the metabolite of the microorganism, any of the culture broth itself and the culture supernatant can be used. This metabolite, in addition to showing growth promotion of the plant,
Those that suppress the growth of weeds and the like are also included. Examples of the microorganism include Enterobacter cloacae (FERM P-888
4) is used. The obtained microbial material is used after being dried and sowed to the root of a plant.

[0015]

The microbial material according to the present invention is obtained by adding a metabolite of a microorganism which can be industrially mass-cultured to the surface of a fertilizer or a porous material, and is useful for promoting plant growth by using it. By keeping the microbial material particle size between 0.1mm and 2mm (90% by weight or more) while maintaining the action of the culture solution of various microorganisms, the quality is stable, and its adjustment and handling are easy and long-term storage Is possible.

[0016]

Next, the present invention will be described with reference to examples. The present invention is not limited to these.

Example (NH ₄ ) ₂ SO ₄ 0.1% KH ₂ PO ₄ 1.0% MgSO ₄ 0.01% Trisodium citrate 0.05% Glucose 0.4% L-tryptophan 3mg / ml (final concentration) 100ml of medium having the above composition Was added to a 1-liter Erlenmeyer flask, sterilized under the conditions of 121 ° C., 1 atm, and 15 minutes, and cooled. Then, Enterobacter cloacae (FERM P-8844), which had been pre-cultured in 3 ml of the above-described medium, was added, and cultured at 37 ° C for 72 hours while shaking at 100 rpm, followed by centrifugation (7000 rpm, 10 minutes, (4 ° C.).

After culturing Enterobacter cloacae (FERM P-8844), a culture solution was obtained. Add 50 ml of culture solution and 5 liters of pure water to a particle size of 10
5 kg of μm silica (Nippon Silica Industrial Product Nip Seal) is charged into the granulator and added while rotating the stirring blade.
At room temperature (15-30 ° C), granulate under the conditions of 1800 rpm for 3-5 minutes, 700 rpm for 2 minutes, and 350 rpm for 2 minutes.
(Particle size: 0.1 to 2 mm; 95% by weight or more). After drying the microbial material, about 6 g was sown at the root when planting Phalaenopsis orchid, and about one month later, the dry weight of the root was measured to be about 5 g on average. (n = 10)

Comparative Example In the example, only 5 L of pure water was used without using a culture solution.
When the same operation was performed, the average dry weight of the root was 3 g. (n = 10)

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Claims (4)

[Claims] A microbial metabolite is added to a filler powder having a particle size of 0.01 mm or less, and granulated by a granulator to reduce the particle size to 0.1 mm.
A method for producing a microbial material, characterized in that the thickness is set to 2 mm (90% by weight or more). 2. The method for producing a microbial material according to claim 1, wherein the filler is a fertilizer or a porous body. 3. The method according to claim 2, wherein the porous body is silica. 4. The method for producing a microorganism material according to claim 1, wherein the metabolite of the microorganism is a microorganism culture solution or a microorganism culture supernatant.