JP2002338595A - DnaA蛋白質とATPとの親和性を利用した、病原性細菌の染色体DNA複製開始蛋白質DnaAの精製方法、並びに、DnaA蛋白質とATPとの親和性を利用した、抗菌活性の評価方法および抗菌活性を有する化合物のスクリーニング方法 - Google Patents

DnaA蛋白質とATPとの親和性を利用した、病原性細菌の染色体DNA複製開始蛋白質DnaAの精製方法、並びに、DnaA蛋白質とATPとの親和性を利用した、抗菌活性の評価方法および抗菌活性を有する化合物のスクリーニング方法

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Publication number
JP2002338595A
JP2002338595A JP2001129934A JP2001129934A JP2002338595A JP 2002338595 A JP2002338595 A JP 2002338595A JP 2001129934 A JP2001129934 A JP 2001129934A JP 2001129934 A JP2001129934 A JP 2001129934A JP 2002338595 A JP2002338595 A JP 2002338595A
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Prior art keywords
protein
dnaa
activity
atp
dnaa protein
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JP2001129934A
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English (en)
Japanese (ja)
Inventor
Kazuhisa Sekimizu
和久 関水
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GENOM SOYAKU KENKYUSHO KK
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GENOM SOYAKU KENKYUSHO KK
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Priority to JP2001129934A priority Critical patent/JP2002338595A/ja
Priority to PCT/JP2002/001913 priority patent/WO2002090548A1/fr
Publication of JP2002338595A publication Critical patent/JP2002338595A/ja
Withdrawn legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/14Streptococcus; Staphylococcus
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18Testing for antimicrobial activity of a material
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911Bacteria
    • G01N33/56938Staphylococcus

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Cell Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
JP2001129934A 2001-04-26 2001-04-26 DnaA蛋白質とATPとの親和性を利用した、病原性細菌の染色体DNA複製開始蛋白質DnaAの精製方法、並びに、DnaA蛋白質とATPとの親和性を利用した、抗菌活性の評価方法および抗菌活性を有する化合物のスクリーニング方法 Withdrawn JP2002338595A (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2001129934A JP2002338595A (ja) 2001-04-26 2001-04-26 DnaA蛋白質とATPとの親和性を利用した、病原性細菌の染色体DNA複製開始蛋白質DnaAの精製方法、並びに、DnaA蛋白質とATPとの親和性を利用した、抗菌活性の評価方法および抗菌活性を有する化合物のスクリーニング方法
PCT/JP2002/001913 WO2002090548A1 (fr) 2001-04-26 2002-03-01 Methode exploitant l'affinite de la proteine d'adna pour l'atp pour purifier une proteine d'adna initiant la replication d'un adn chromosomique d'une bacterie pathogene, methode d'evaluation d'une activite antibacterienne, et methode utilisant l'affinite de la proteine d'adna pour l'atp pour cribler un compose ayant une act

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2001129934A JP2002338595A (ja) 2001-04-26 2001-04-26 DnaA蛋白質とATPとの親和性を利用した、病原性細菌の染色体DNA複製開始蛋白質DnaAの精製方法、並びに、DnaA蛋白質とATPとの親和性を利用した、抗菌活性の評価方法および抗菌活性を有する化合物のスクリーニング方法

Publications (1)

Publication Number Publication Date
JP2002338595A true JP2002338595A (ja) 2002-11-27

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ID=18978387

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JP2001129934A Withdrawn JP2002338595A (ja) 2001-04-26 2001-04-26 DnaA蛋白質とATPとの親和性を利用した、病原性細菌の染色体DNA複製開始蛋白質DnaAの精製方法、並びに、DnaA蛋白質とATPとの親和性を利用した、抗菌活性の評価方法および抗菌活性を有する化合物のスクリーニング方法

Country Status (2)

Country Link
JP (1) JP2002338595A (fr)
WO (1) WO2002090548A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4468299B2 (ja) * 2003-05-30 2010-05-26 塩野義製薬株式会社 細菌の増殖抑制剤のスクリーニング方法
CN107022644B (zh) * 2017-06-14 2021-01-05 山东省农业科学院农业质量标准与检测技术研究所 果蔬中六种食源性致病菌多重lamp检测引物、检测试剂盒及检测方法

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