JP2002325541A - Feed for poultry - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a feed for poultry that includes a safe and effective substance as a replacement for an antibiotic viable micrope preparation, oligosaccharide, etc., for prophylaxis of salmonellosis in poultry and inhibition of salmonella excretion from poultry. SOLUTION: An essence from sugar cane is fed to poultry as an active ingredient.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a poultry feed to which a sugar cane-derived extract is added to prevent Salmonella infection or suppress Salmonella excretion.

[0002]

2. Description of the Related Art The occurrence of food poisoning due to salmonella, which is a food product caused by livestock products, has become a public health problem. In particular, in recent years, the occurrence of food poisoning of salmonella caused by chicken eggs has increased. Salmonella food poisoning is the third of all food poisoning cases
More than 40% of the total number of bacterial food poisoning patients
It is said to occupy 45% ("Information on the detection of pathogenic microorganisms", National Institute of Health), 18 (3), P.51-52,
(1991), and "Chicken Meat Information", September 25, 2000, p.
27-28).

[0003] Salmonella, which has low pathogenicity to poultry and causes food poisoning in humans, often causes the disease to pass through, and is often carried out in a carrier state. Such poultry causes food poisoning through meat and eggs. Is a problem. Therefore, research is being conducted not only on the treatment of salmonellosis but also on the prevention of infection or transmission. To prevent food poisoning caused by livestock products, antibacterial substances such as antibiotics and synthetic antibacterial agents are widely used in livestock and poultry for the purpose of treatment or prevention. Due to problems such as the emergence of, its use is severely restricted. Also, even within the usable range, the use of such antibacterial substances tends to be undesired in view of the natural and natural consciousness of consumers in recent years. In addition to the use of antibacterial substances, the use of natural substances such as viable bacterial preparations and oligosaccharides is also spreading, but has not been effective in preventing and treating Salmonella infection and solving the problem of food poisoning. An inactivated vaccine of Salmonella enteritidis (hereinafter referred to as SE) is also commercially available, but its effect is to reduce the colonization of SE in the chicken intestinal tract and cannot completely protect SE.

[0004] Examples of the preventive and therapeutic agents for salmonellosis derived from natural products that have been reported so far include the following. Japanese Patent Application Laid-Open No. 62-181218 discloses an infection protective agent containing a neutral polysaccharide obtained from a hot water extract of the bark of Meria azadirachta. In this publication, as a confirmation of the effect, only an infection protection test using a mouse is performed, and its efficacy in poultry is unknown. JP-A-1-172329 and JP-A-1-172330
In Japanese Patent Application Laid-Open Publication No. H10-157, a preventive / therapeutic agent for Salmonella typhimurium infection containing Gobishi is described. Gobishi is a plant insect ray and is known as one of the crude drugs. JP-A-59-128333 describes an agent for promoting the growth of poultry and preventing and treating Salmonella infection, comprising a specific Clostridial cell as an active ingredient.

An invention in which an extract derived from cane sugar is used for deodorizing livestock excrement and the like is described in, for example, JP-A-11-155497. In addition, the invention using a sugarcane-derived extract as a prophylactic / therapeutic agent for infection is disclosed in, for example, JP-A-2000-29.
No. 7046 publication. According to this, it is described that an extract derived from cane sugar has a preventive and therapeutic effect on various bacterial infections. In the detailed description of the invention of this publication, a sugar cane-derived extract shows a preventive and therapeutic effect on infection with respect to bacteria, for example, human salmonellosis (Salmonella enteritidi).
s, S. dublin, etc.) and chicken chick dysentery (Salmonel)
la pullorum).

[0006]

SUMMARY OF THE INVENTION Antibiotics, viable bacterial preparations,
Instead of oligosaccharides, etc., to prevent the transmission of Salmonella in poultry, or to control the eradication of Salmonella,
Safe, natural, and effective substances have not been known before.

[0007]

Means for Solving the Problems The present inventors have conducted intensive studies in order to solve the above-mentioned problems, and as a result, by continuously feeding poultry with a feed containing an extract derived from sugar cane as an active ingredient, Salmonella The present inventors have found that the infection-preventing effect and the salmonella excretion-suppressing effect are clearly exhibited, and completed the present invention. That is, the present invention aims to prevent Salmonella infection or suppress Salmonella excretion,
Poultry feed to which cane-derived extract is added.

The sugarcane-derived extract according to the present invention is disclosed in
000-297046 or JP-A-11-15
No. 5497 is an extract derived from sugar cane,
More specifically, any one of a fraction obtained by column chromatography using sugar cane juice or molasses derived from sugar cane as a raw material, a synthetic adsorbent or a cation exchange resin as a fixed carrier, and a hot water extract of bagasse Or a mixture thereof.

[0009] The form of the extract of the present invention derived from sugar cane according to the present invention is not particularly limited, and the extract in liquid or powder form may be added as it is. Can be made into a solid preparation or a liquid preparation by a known method.

Examples of liquid sugar cane-derived extracts include sugarcane extract (trade name: sugarcane extract MSX-).
1 L, manufactured by Shin-Mitsui Sugar Co., Ltd.) or a desugared sugarcane extract (trade name: Kibi Shibori EX-L, manufactured by Shin-Mitsui Sugar Co., Ltd.) can be used.

When a solid preparation is used, for example, a preparation in which corn starch is mixed with an extract derived from cane sugar, or a preparation in which defatted rice bran is mixed with an extract derived from cane sugar can be prepared. Examples of such a preparation include, for example, a preparation prepared by adsorbing a liquid sugarcane extract (trade name: Sugarcane extract MSX-1L, manufactured by Shin Mitsui Sugar Co., Ltd.) onto corn starch, or a dried sugarcane extract (defatted rice bran). A granule preparation (trade name: Kibi-Shibori EX, Shin-Mitsui Sugar Co., Ltd.) that adsorbs and drys (trade name: Kibi Shibori EX-L, Shin-Mitsui Sugar Co., Ltd.) can be used. EX
Is a preparation containing 200 mg ± 20 mg of solid content excluding the monosaccharides and oligosaccharides of EX-L in 1 g thereof.

The extract of sugarcane-derived extract is a natural product, and therefore its analytical value is not constant depending on the locality of production of sugarcane, climate, etc., for example, one analytical value of liquid sugarcane extract MSX-1L Is as follows.
The solid content by freeze-drying is 29.4%, the sugar content is 2.5% per solid content, breakdown of sucrose 1.8%, glucose 2.3%, fructose 0.4%, polyphenol measurement by folin-thiocult method. The value was 23.0% per solid based on catechin. In addition, squeezing E
One analysis value of XL is as follows. Solid content by a normal pressure heating drying method is 37.4%, and Brix is Bx. 4
0.6, sugar content 9.8% per solid content, sucrose 6.8%, glucose 1.4%, fructose 1.6%, polyphenol measured by folin-thiocarte method 5.0%.

Such sugarcane-derived extracts are natural because they are derived from plants, and are low in cost because they utilize components that are not necessary in the process of producing sugar, which is usually the purpose of cultivating sugarcane. In addition, since it is a component contained in sugar cane and honey-bearing sugar that have been eaten by humans since ancient times, it can be said that it is safe with experience in eating.

[0014] The feed according to the present invention means all that is orally ingested by poultry for nutritional purposes. More specifically, when classified in terms of nutrient content, rough feed, concentrated feed, mineral feed and special feed can be used. Including all of them and classifying them according to official standards, they include all of compound feeds, mixed feeds and single feeds. Further, when classified in terms of feeding method, it includes all feeds directly fed, mixed with other feeds and fed, or added to drinking water to supplement nutrients.

The feed of the present invention is a feed for poultry to which an extract derived from sugar cane or an extract preparation derived from sugar cane as described above, such as EX-L or EX, is added. Specifically, for example, in the case of a solid feed containing EX-L, 0.001 to 0.05% by weight, preferably 0.003% by weight of EX-L as a solid content excluding monosaccharides and oligosaccharides. -0.05% by weight, more preferably 0.005-0.01% by weight. In the case of feed added to drinking water, EX-L per liquid weight of drinking water is 0.0005-0.005.
0.025% by weight, desirably 0.0015 to 0.02
5% by weight, more preferably 0.0025 to 0.005
It is a feed to which weight% is added. The amount of feed given to chickens is
325 g per day, 1 kg body weight, chicks,
It weighs 28 g for an adult chicken. Based on the above, the extract intake per unit weight of poultry is calculated, and the feed of the present invention is, specifically, an extract derived from sugar cane, for example,
XL is 0.2 to 200.0 m / day as a solid content excluding monosaccharides and oligosaccharides per 1 kg body weight of poultry.
g, preferably 1.0 to 200.0 mg, more preferably 2.0 to 40.0 mg.

In the method of feeding a feed of the present invention, it is desirable to administer poultry continuously or orally at the start of breeding. In the case of feed added to drinking water, intensive administration is performed at a specific time. Is also good.

According to the examples described below, the feed of the present invention is fed and at the same time artificially infected with Salmonella, the excretion of Salmonella in poultry is suppressed, and the cecal stool or cecal contents of the poultry used is suppressed. When the positive rate was measured, it was lower than that in the control group to which a normal feed containing no cane-derived extract was given. This suggests that a feed supplemented with cane-derived extract not only suppresses Salmonella excretion but also makes it difficult for Salmonella in the poultry to colonize, thus preventing the transmission of Salmonella to poultry. And inhibits the growth of Salmonella in poultry. In addition, since the amount of Salmonella in feces is reduced, it is possible to prevent the transmission of Salmonella infection using Salmonella in feces as a source of infection.

[0018]

EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples.

Example 1 One-day-old male laying hens (High Line Roller) were used in 30 chickens per test plot, and each test plot contained 5 birds in 6 cages. The feed is a standard feed for young chicks SDL No. 1 (manufactured by Nippon Combined Feed Co., Ltd.), and in each test plot, from 1-day to 43-day and 47-day-old, constant feeding and watering were used.
From the 4th to the 46th day, food and water were deprived. The test category is SDL No. As a control group fed only 1 and a feed feeding group added with an extract derived from sugar cane (a group administered with an extract preparation derived from sugar cane), 0.025% by weight of Kibishibori EX (trade name, Shin Mitsui Sugar Co., Ltd.) was added. 0.02
5% section, 0.05% section added with 0.05% by weight, 0.1%
The test was divided into a 0.1% section with addition of 0.2% by weight and a 0.25% section with addition of 0.25% by weight. Each test group was orally infected with 1 × 10 ⁵ SE per bird on the 5th day. Samples for SE measurement were 13 days old, 27
For the age of the day and the age of 34, the entire amount of cecal stool excreted from the day before the measurement to the day of each cage was taken. 4
Regarding the 0-day and 47-day-olds, 15 birds were dissected in each test group, and the entire contents of the cecum were collected for each individual sample. As a method for measuring SE, the sample was inoculated into a 10-fold amount of buffered peptone water and cultured at 37 ° C. for 20 hours. After the culture, 1 ml of the culture was inoculated into 10 ml of a harnatetrathionate medium (hereinafter referred to as HTT) and cultured at 43 ° C. for 24 hours. Further, one platinum loop was taken, smeared on a DHL agar medium, cultured at 37 ° C. for 24 hours, and the properties of black colonies on the agar were checked to confirm that they were SE. (This is the primary culture.) For samples in which SE was not detected in the primary culture, 2
After 4 hours of culture, the HTT was further cultured at room temperature for 5 days,
0.5 ml of the culture was inoculated into 4.5 ml of fresh HTT and cultured at 43 ° C. for 24 hours. After the cultivation, one platinum loop was removed and smeared on a DHL agar medium, and SE was detected (delayed secondary enrichment culture) to make a final judgment. The test chicks were not infected with Salmonella at the age of 1 day. Tables 1 and 2 show the detection status of SE.

[Table 1]

[Table 2] As shown in Table 1, looking at the results of the 34-day age, 0.0
The number of SE positives in the 5% group was 4 compared to the 6 positive cases in the control group.
Cases decreased. Further, as shown in Table 2, 0.05
In the% plot, the number of positives in the 40-day-old decreased from 7 cases to 15 in the control plot, and even in the 47-day-old, the number of positives in the control plot decreased from 7 to 15 in the control plot. Clearly, excretion of SE was suppressed. A decrease in the detection rate of SE from cecal contents also means that the colonization of SE in poultry bodies was suppressed. That is, it was revealed that the sugarcane-derived extract can suppress the excretion of salmonella in poultry and prevent the infection of salmonella.

Example 2 One-day-old male hens (High Line Roller) were used in each test group, and each test group housed five chickens in four cages. The feed is a standard feed for young chicks SDL No. 1 (manufactured by Nippon Combined Feed Co., Ltd.), and in each test plot, 1-day to 43-day and 47-day were fixed feed and water-supply, and 44-day to 46-day were fasted and water-free. did. The test category is SDL No. No. 1 as a control group, and a cane extract MSX as a feed-feeding group to which a sugar cane-derived extract was added (a cane-derived extract preparation administration group).
-1L (trade name, Shin Mitsui Sugar Co., Ltd.) was adsorbed and dried on cornstarch so that the solid content excluding the monosaccharides and oligosaccharides was 10% per the solid content of the formulation.
The test was performed in a 0.05% section where 5% by weight was added and in a 0.1% section where 0.1% by weight was added. The sample collection method, the SE culture method, and the determination method were performed in the same manner as in Example 1. Tables 3 and 4 show the detection status of SE.

[Table 3]

[Table 4] As shown in Table 3, the results of the 27-day and 34-day ages showed that the number of SE-positives in the 0.1% ward decreased to half. Further, as shown in Table 4, in the 0.1% group, at the age of 40 days, the number of positive groups was 4 and the number of positive groups was 47 compared to 10 in the control group.
In the old age, excretion of SE was clearly suppressed, with 5 positive cases compared to 9 positive cases in the control plot. SE from cecal contents
Decreasing the detection rate also means that the colonization of SE in poultry bodies was suppressed. That is, it was revealed that the sugarcane-derived extract can suppress the excretion of salmonella in poultry and prevent the infection of salmonella.

[0021]

According to the present invention, a feed containing an extract derived from sugar cane showed an effect of suppressing excretion of salmonella in poultry. Thus, the feed to which the cane-derived extract is added can prevent transmission of Salmonella infection to other poultry via feces. In addition, the fact that the feed of the present invention is fed and at the same time artificially infects Salmonella to inhibit salmonella excretion means that the feed of the present invention can suppress the colonization of Salmonella in poultry bodies. From the above, the occurrence of food poisoning caused by Salmonella can be reduced. In addition, the sugar cane-derived extract of the present invention is derived from plants and is a natural product contained in sugar cane and bee sugar that humans have eaten since ancient times.
Safe and low cost without harm to human and animal health. In addition, despite being a natural product, the effect of suppressing the excretion of Salmonella is high, and it works in small amounts,
Very useful in industry.

 ────────────────────────────────────────────────── ─── Continuing on the front page (72) Inventor Toshiaki Yamamoto 1352-1 Hirai, Kashima-shi, Ibaraki Pref. Inventor Takeo Mizutani 1194-33, Hazawacho, Kanagawa-ku, Yokohama-shi, Kanagawa F term (reference) 2B005 DA01 DA05 2B150 AA05 AB10 DD31 DD57 4C088 AB73 BA08 MA02 NA14 ZB35 ZC61

Claims (6)

[Claims] 1. A poultry feed to which an extract derived from sugar cane has been added for the prevention of Salmonella infection or the suppression of Salmonella excretion. 2. The poultry feed according to claim 1, which is used for poultry selected from chickens, ducks, quails, and turkeys. 3. An extract derived from cane sugar is added so that 0.2 to 200.0 mg of a solid content excluding monosaccharides and oligosaccharides per 1 kg of poultry body weight per day is administered.
The poultry feed according to claim 1 or 2. 4. An extract derived from cane sugar is added so as to be administered in an amount of 1.0 to 200.0 mg as a solid content excluding monosaccharides and oligosaccharides per 1 kg of poultry body weight per day.
The poultry feed according to claim 1 or 2. 5. The method according to claim 1, wherein the sugarcane-derived extract is added in an amount of 2.0 to 40.0 mg as a solid content excluding monosaccharides and oligosaccharides per 1 kg of poultry body weight per day. Poultry feed. 6. The poultry feed according to claim 1, wherein Salmonella is a causative bacterium of human food poisoning.