JP2002315579A5 - - Google Patents

Claims (10)

DNA selected from the following group.
(A) comprising one or a plurality of base sequences represented by nucleotide numbers 1 to 1380 of SEQ ID NO: 41 in the sequence listing, and being introduced into the ML-236B producing microorganism, DNA characterized by promoting synthesis:
(B) DNA that hybridizes with the DNA described in (a) under stringent conditions and is introduced into an ML-236B-producing microorganism to promote ML-236B biosynthesis of the producing microorganism . E. coli transformed E. coli coli pSAKexpR SANK 72599 strain can be obtained from (FERM BP-7006), according to claim 1 DNA according. A recombinant DNA vector comprising the DNA according to claim 1 or 2 . E. coli transformed E. coli The recombinant DNA vector according to claim 3 , which can be obtained from E. coli pSAKexpR SANK 72599 (FERM BP-7006). A host cell transformed with the recombinant DNA vector according to claim 3 or 4 . The host cell according to claim 5, which is an ML-236B producing microorganism. 7. Host cell according to claim 6 , characterized in that it is Penicillium citrinum. A method for producing ML-236B, comprising culturing the host cell according to claim 6 or 7 , and then recovering ML-236B from the culture. The host cell according to claim 5 , which is Escherichia coli. E. coli transformed E. coli 10. The host cell according to claim 9 , which is E. coli pSAKexpR SANK 72599 (FERM BP-7006) strain.