JP2002291442A - Phytosterol-containing water-soluble composition, method for producing the same and use thereof - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a phytosterol-containing water-soluble composition storable over a long period without causing the isolation or precipitation of phytosterol crystal, keeping uniform and stable emulsified, dispersed or solubilized state and having excellent acid resistance, salt resistance and heat-resistance necessary as an additive for foods and drinks. SOLUTION: The composition is composed of (A) a phytosterol, (B) one or more oleophilic emulsifiers selected from glycerol fatty acid esters, organic acid monoglycerides, polyglycerol fatty acid esters, propylene glycol fatty acid esters, polyglycerol condensed ricinoleic acid esters, sorbitan fatty acid esters, sucrose fatty acid esters and lecithins, (C) edible oils, (D) at least one kind of hydrophilic polyglycerol fatty acid ester and (E) water. The HLB value of the emulsifier in the total system is >=12.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a plant sterol-containing water-soluble composition, a method for producing the same, and a food or drink containing the composition. More specifically, the plant sterol does not precipitate or float even after long-term storage, maintains a homogeneous emulsified, dispersed or solubilized state, and is required for heat resistance, acid resistance, and salt resistance required when added to foods and drinks. The present invention relates to a plant sterol-containing water-soluble composition, a method for producing the same, and a food or drink containing the composition.

[0002]

2. Description of the Related Art Plant sterols are a general term for various sterols and mixtures thereof contained in plants, and β-sitosterol, stigmasterol, campesterol, brassicasterol and the like are known as main components. BACKGROUND ART Plant sterols have long been known to have a plasma cholesterol concentration lowering effect and the like, and recently, many research results have been reported such as an effect of improving colon cancer and prostatic hypertrophy. Therefore, it has been greatly expected that plant sterols having such excellent physiological effects are added to foods and drinks, particularly to beverages. However, plant sterols, due to their structure, have extremely low solubility in oils, solvents and water, strong crystallinity, high melting points (130-150 ° C.)
Therefore, it has been difficult to uniformly disperse in water and use it as a stable liquid product. For this reason, in pharmaceuticals and foods, they have been suspended in oil or provided in the form of powder or granules.However, the poor absorption of plant sterols into the human body as it is insolubilized, Poor workability at the time of use has become a problem, and improvements have been demanded.

There have been several reports on the solubilization of plant sterols. Japanese Patent Publication No. 6-59164 discloses a water gel composition containing a sterol, a lipophilic emulsifier, and a fat or oil as a composition having good stability. However, in the technique described above, when used as an improver for processed foods and the like, the gel collapses due to dilution, and sterol crystals are precipitated. Further, in actual distribution, there is a problem of storage stability such as spoilage of the gel composition, and the stability is not always satisfactory. JP 2001
No. 117 discloses an oil-in-water emulsion composition containing a plant sterol, lecithin, ethanol, and the like. However, when this oil-in-water emulsion composition is diluted with water and heat-treated, sterol crystals are precipitated, and there is a problem in heat stability of the emulsion composition. Therefore, in the prior art, the properties required when added to food and drink, acid resistance, salt resistance, both excellent heat resistance, stable emulsification, sufficient as a composition to maintain a dispersed or solubilized state. Nothing was satisfactory. In addition, as a problem due to the intake of plant sterols, there is a decrease in the absorption efficiency of fat-soluble vitamins.
JP-A-179086 discloses a food and drink and an oil solubilized solution containing a plant sterol, vitamin E and a surfactant. However, according to the above-mentioned technology, oil-soluble ones and water-soluble ones do not always provide satisfactory liquid stability. Therefore, at present, a water-soluble composition containing a plant sterol having excellent stability and a water-soluble plant sterol composition containing an oil-soluble vitamin are desired.

[0004]

The first object of the present invention is to provide a water-soluble composition of the poorly soluble plant sterol as described above to enable its use in a wide range of fields such as pharmaceuticals and foods. Even when stored for a long period of time, it does not release or precipitate plant sterol crystals, maintains a homogeneous and stable emulsified, dispersed, solubilized state, and has acid resistance properties required when added to foods and drinks An object of the present invention is to provide a plant sterol-containing water-soluble composition having excellent salt resistance and heat resistance. Still further, a second object of the present invention is to incorporate one or more fat-soluble vitamins into the plant sterol-containing water-soluble composition, thereby inhibiting the absorption of fat-soluble vitamins at the time of plant sterol ingestion. An object of the present invention is to provide a water-soluble composition containing a fat-soluble vitamin and a plant sterol, which has reduced the problem. Further, a third object of the present invention is to provide a method for producing the water-soluble composition. Further, a fourth object of the present invention is to provide a food or drink containing the water-soluble composition.

[0005]

Means for Solving the Problems The present inventors have made intensive studies to solve the above-mentioned problems, and as a result, according to a specific formulation and a specific production method, a plant sterol-containing water-soluble composition having excellent stability was obtained. It was found that a product was obtained, and the present invention was completed. That is, the present invention includes the following [1] to [7].

[1] (A) plant sterol, (B) glycerin fatty acid ester, organic acid monoglyceride, polyglycerin fatty acid ester, propylene glycol fatty acid ester, polyglycerin condensed ricinoleate, sorbitan fatty acid ester, sucrose fatty acid ester and lecithin A lipophilic emulsifier selected from the group consisting of (C) edible oils, (D) at least one hydrophilic polyglycerin fatty acid ester, and (E) water. A plant sterol-containing water-soluble composition wherein the HLB value of the emulsifier in the entire system is 12 or more.

[2] (A) Plant sterol 0.05 to 5
0% by weight (B) one or more selected from the group consisting of glycerin fatty acid ester, organic acid monoglyceride, polyglycerin fatty acid ester, propylene glycol fatty acid ester, polyglycerin condensed ricinoleic acid ester, sorbitan fatty acid ester, sucrose fatty acid ester and lecithin 0.01 to 50% by weight of two or more lipophilic emulsifiers, (C) 0.01 to 50% by weight of edible oils, (D) 0.5 to 50% by weight of at least one hydrophilic polyglycerin fatty acid ester And (E) a plant sterol-containing water-soluble composition wherein the emulsifier in the entire system has an HLB value of 12 or more, which comprises a water residue.

[3] One or more fat-soluble vitamins selected from the group consisting of (F) vitamin A, vitamin D, vitamin E and vitamin K are further added to the plant sterol-containing water-soluble composition. A plant sterol-containing water-soluble composition.

[4] One or two or more fat-soluble vitamins selected from the group consisting of (F) vitamin A, vitamin D, vitamin E and vitamin K in the plant sterol-containing water-soluble composition. 0.01-12.5% by weight
And a plant sterol-containing water-soluble composition.

[5] The method for producing a water-soluble composition containing a plant sterol, which comprises: (A) a plant sterol,
(B) one or more lipophilic emulsifiers and (C)
An oil phase containing edible oils and an aqueous phase comprising (D) at least one hydrophilic polyglycerin fatty acid ester and (E) water are blended and homogenized, and the HLB of the emulsifier in the whole mixture is obtained. A method for producing a plant sterol-containing water-soluble composition, wherein the value is 12 or more.

[6] A method for producing a water-soluble composition containing the above-mentioned plant sterol, comprising: (A) a plant sterol, (B) one or more lipophilic emulsifiers, (C)
An oil phase containing edible oils and (F) one or more fat-soluble vitamins selected from the group consisting of vitamin A, vitamin D, vitamin E, and vitamin K;
(D) At least one hydrophilic polyglycerol fatty acid ester and (E) an aqueous phase composed of water are blended and homogenized, and the HLB of the emulsifier in the whole mixture system is mixed.
A method for producing a plant sterol-containing water-soluble composition having a value of 12 or more. [7] A food or drink containing the plant sterol-containing water-soluble composition.

[0012]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail.
The (A) plant sterol used in the present invention is, for example, extracted and purified from vegetable oils such as soybeans, rapeseed, and cottonseed, and contains β-sitosterol, stigmasterol, campesterol, brassicasterol and the like as main components. Mixture. In the present invention, the content of the plant sterol is usually 0.05 to 50% by weight, preferably 0.1 to 50% by weight.
It is 30% by weight, more preferably 1 to 10% by weight. When the plant sterol content is less than 0.05% by weight, the physiological effect as a plant sterol is small, and
If the amount is more than 10% by weight, the emulsion stability of the composition is poor, which is not preferable.

The lipophilic emulsifier (B) used in the present invention is H
It has an LB value of less than 8, and is selected from, for example, glycerin fatty acid ester, organic acid monoglyceride, polyglycerin fatty acid ester, propylene glycol fatty acid ester, polyglycerin condensed ricinoleate, sorbitan fatty acid ester, sucrose fatty acid ester and lecithin. One or more emulsifiers. More preferred are organic acid monoglycerides, propylene glycol fatty acid esters, and sucrose fatty acid esters.
Further, the above-mentioned emulsifier is not necessarily required to be highly purified by distillation or the like, and may be a reaction mixture. The compounding amount of the lipophilic emulsifier used in the present invention is usually 0.01 to 50% by weight, preferably 1 to 20% by weight, more preferably 1.5 to 10% by weight. If the amount of the lipophilic emulsifier exceeds 50% by weight, the taste specific to the lipophilic emulsifier affects the taste of the food or drink when the plant sterol-containing water-soluble composition is added to the food or drink. On the other hand, if the amount of the lipophilic emulsifier is less than 0.01% by weight, the emulsion stability is poor, which is not preferable. Commercially available lipophilic emulsifiers include Riquemar PO-100 (RIKEN Vitamin Co., Ltd.)
HLB 3.4), Sunsoft No. 661AS
(Manufactured by Taiyo Chemical Co., Ltd., HLB7.5) Ryoto Sugar Ester P-170 (manufactured by Mitsubishi Chemical Corporation, HLB
1.0) and the like.

The edible oils (C) used in the present invention include:
For example, oils and fats or synthetic oils made from animals, plants and microorganisms. Specifically, for example, lard, beef tallow, chicken oil, whale oil, tuna oil, sardine oil, mackerel oil, saury oil, skipjack oil, herring oil, liver oil, soybean oil, cottonseed oil, safflower oil,
Rice oil, corn oil, rapeseed oil, palm oil, perilla oil, perilla oil, cocoa butter, peanut oil, coconut oil, evening primrose oil, borage oil, jojoba oil, etc., and medium-chain fatty acid triglycerides (hereinafter abbreviated as MCT oil) And the like triglyceride. These fats and oils can be used alone or in an appropriate combination. Further, it may be edible oil alone or a food additive or the like. The edible oil is more preferably soybean oil,
Edible oils that are liquid at normal temperature such as cottonseed oil, safflower oil, rice oil, corn oil, rapeseed oil, perilla oil, perilla oil, evening primrose oil, borage oil, and more preferably soybean oil, corn oil,
Rapeseed oil and MCT oil. The amount of the edible oil is usually 0.01 to 50% by weight, preferably 1 to 50% by weight, more preferably 2.5 to 50% by weight based on the total water-soluble composition.
40% by weight. If the content of edible oils is 50% by weight or less, edible oils and fats can be easily and stably emulsified.

The (D) hydrophilic polyglycerin fatty acid ester used in the present invention includes polyglycerin having an average degree of polymerization of 2 or more and a fatty acid having 8 to 18 carbon atoms, such as caprylic acid, capric acid, lauric acid, myristic acid, and palmitic acid. Esters with acids, stearic acid, oleic acid, and linoleic acid include at least one compound selected from among hydrophilic emulsifiers having an HLB value of 8 or more, preferably 12 or more. A monoglycerin fatty acid ester makes it difficult to obtain a uniform plant sterol-containing water-soluble composition, and when it is other than a fatty acid having 8 to 18 carbon atoms, emulsification becomes unstable, which is not preferable. In the present invention, this polyglycerin fatty acid ester can be used alone or in an appropriate combination. As a commercial product, for example,
Sunsoft Q-17UL (manufactured by Taiyo Kagaku Co., Ltd., HLB
19) Poem DO-100 (manufactured by Riken Vitamin Co., Ltd.)
HLB8), Poem J-0021 (RIKEN Vitamin Co., Ltd.)
HLB16), Sunsoft Q-14S (HLB14.5, manufactured by Taiyo Chemical Co., Ltd.), Sunsoft A-141
C (manufactured by Taiyo Chemical Co., Ltd., HLB12). The amount of the hydrophilic polyglycerin fatty acid ester used in the present invention is usually 0.5 to 50% by weight, preferably 2 to 30% by weight, more preferably 5 to 25% by weight. When the blending amount of the polyglycerin fatty acid ester is less than 0.5% by weight, it becomes difficult to stably emulsify, disperse, and solubilize the plant sterol.
When the water-soluble composition containing a plant sterol is added to foods and drinks, the characteristic taste of polyglycerin fatty acid ester affects the taste of foods and drinks, which is not preferable.

The lipophilic emulsifier (B) and the hydrophilic polyglycerol fatty acid ester (D) used in the present invention are used as the emulsifier HLB in the whole water-soluble composition containing plant sterols.
It is blended so that the value is 12 or more. When the HLB value is less than 12, it becomes difficult to stably emulsify, disperse, and solubilize the plant sterol, and the heat resistance, acid resistance, and salt resistance are poor. Here, HLB is a balance between hydrophilicity and hydrophobicity usually used in the field of surfactants, and a commonly used formula can be used. Further, as described above, numerical values of HLB described in catalogs of commercial products and the like may be used. Also,
Regarding the lipophilic emulsifier of the component B and the hydrophilic polyglycerin fatty acid ester of the component D, the HLB used in the system may be finally 12 or more based on the respective HLB and the amount added. For example, the above-mentioned commercially available products include Riquemar PO-100.
(HLB3.4) and Sunsoft Q-17UL (HLB1
HLB to 15 by combination of 9), Poem DO-
100 (HLB8) and Sunsoft Q-17UL (HLB
HLB to 15 by the combination of Sunsoft No. 19). 661AS (HLB7.5) and Sunsoft Q-1
HLB13 by combination with 4S (HLB14.5)
May be adjusted.

The water (E) used in the present invention is not particularly limited as long as it can be blended into foods and drinks.
It may be water to which food additives are added. The amount of water
In the remainder, although not particularly limited, for example, the blending amount of water is
1 to 50% by weight is desirable. The blending amount of water is more preferably 5 to 25% by weight.

As the (F) fat-soluble vitamins used in the present invention, for example, powdery or liquid vitamin A, vitamin D, vitamin E and vitamin K may be used alone or in combination of two or more. Can be used. The amount of the fat-soluble vitamin used in the present invention is usually 7
2.5% by weight or less, preferably 0.01 to 20% by weight,
More preferably, it is 0.05 to 10% by weight. When the content of the fat-soluble vitamin is 72.5% by weight or less, the fat-soluble vitamin can be easily emulsified stably.

Further, within a range that does not impair the effects of the present invention,
Commonly known food additives may be added. Food additives include emulsifiers, stabilizers, seasonings, acids and salts and the like. As the emulsifier, one or more other emulsifiers can be blended in addition to the polyglycerol fatty acid ester used in the present invention. For example, saponin, sterol,
Cholic acid, desoxycholic acid, Yucca extract and the like.

Further, a stabilizer can be added to the water-soluble composition of the present invention. Examples of the stabilizer include ethylene glycol, propylene glycol, glycerin, pentaerythritol, maltitol, reduced starch syrup, lactitol, palatinit, erythritol, sorbitol, mannitol, fructose fructose syrup, gum arabic, xanthan gum, tragacanth gum, guar gum,
Gellan gum, locust bean gum and the like. The above stabilizers can be used alone or in combination of two or more.

The method for producing the plant sterol-containing water-soluble composition of the present invention is a method in which the above-mentioned various components are measured in a container and mechanically homogenized. In particular, (A) a vegetable sterol, (B) an oil phase containing a lipophilic emulsifier and (C) an edible oil, and (D) at least one oil phase.
-Type hydrophilic polyglycerin fatty acid esters and (E)
This is a production method in which an HLB value of the emulsifier in the whole mixture is 12 or more by blending an aqueous phase composed of water and performing homogenization treatment. Particularly, an oil phase containing (A) a plant sterol, (B) a lipophilic emulsifier, (C) an edible oil and (F) a fat-soluble vitamin, and (D) at least one hydrophilic polyglycerin fatty acid. An ester and an aqueous phase composed of (E) water were blended and homogenized to obtain an emulsifier having an HLB value of 12 in the whole mixture.
The manufacturing method is as described above.

In the production method of the present invention, a homogenizer such as a colloid mill, a high-pressure homogenizer, a microfluidizer, an ultimateizer, or a nanomizer can be used to enhance the stability of emulsification, dispersion or solubilization. Thereby, a uniform liquid plant sterol-containing water-soluble composition can be obtained. In order to further stabilize the plant sterol-containing water-soluble composition, the homogenization treatment may be performed twice or more.

For the homogenization treatment, a homogenization treatment machine such as an ultrasonic emulsification machine can be used in addition to the above-mentioned homogenization treatment machine.
A T emulsification method or the like can also be used.

The plant sterol-containing water-soluble composition of the present invention can be taken as it is, or can be taken in combination with other foods and drinks.

Examples of the foods and drinks include breads and confectioneries such as bread, biscuit, candy and jelly; processed milk products such as yogurt and ham;
Seasonings such as sauces, sauces and dressings; processed foods such as tofu and noodles; margarine, fat spread,
Oil and fat processed foods such as shortening; powdered foods such as powdered beverages and powdered soups; health foods in capsules, tablets, powders, granules, and the like.

Examples of the beverages described above include beverages containing at least one selected from the group consisting of minerals such as salt, acidulants, sweeteners, alcohols, vitamins, flavors and fruit juices, such as sports drinks, carbonated drinks and fruit juices. Beverages,
In addition to lactic acid bacteria drinks, alcoholic drinks, vitamin / mineral drinks, etc., processed milk, soy milk, drinks for improving the constitution, and the like can be mentioned.

[0027]

The plant sterol-containing water-soluble composition of the present invention can maintain a homogeneous and stable state without precipitation or floating of plant sterol even after long-term storage. It also has excellent acid resistance, salt resistance, and heat resistance, and can maintain stability even when an acid or salt used in foods and food additives is blended. High-temperature sterilization or sterilization at 100 to 150 ° C., and is stable against heating at this time, and can maintain a uniform state even when stored for a long time. In addition, the water-soluble composition containing fat-soluble vitamins and plant sterols of the present invention reduces the problem of inhibiting the absorption of fat-soluble vitamins during ingestion of plant sterol, and the above-mentioned water-soluble composition containing plant sterols It has the functionality of
In addition, the method for producing the plant sterol-containing water-soluble composition of the present invention is a simple method for setting a specific HLB, and particularly, a lipophilic emulsifier and a hydrophilic emulsifier are blended, so that a water-soluble composition having excellent stability is obtained. This is the method by which the composition is obtained. These water-soluble compositions containing plant sterols and / or water-soluble compositions containing fat-soluble vitamins and plant sterols can be ingested as they are as foods. And / or a food or drink containing fat-soluble vitamins and plant sterols can be obtained.

[0028]

Next, the present invention will be described in more detail with reference to Examples and Comparative Examples. Example 1 Decaglycerin monolaurate [trade name: Poem J-0]
021, manufactured by Riken Vitamin Co., Ltd., HLB16] 115
g, water 140 g, glucose fructose liquid sugar [trade name: KM-F]
55, manufactured by Nippon Shokuhin Kogyo Co., Ltd.]
The mixture was completely dissolved by heating to obtain an aqueous phase. Aside from this,
Propylene glycol monooleate [trade name: Riquemar PO-100 manufactured by Riken Vitamin Co., Ltd., HLB3.
4] 30 g, 285 g of medium-chain fatty acid triglyceride [trade name: Panassate 810 manufactured by NOF CORPORATION] was heated,
While stirring, 35 g of a plant sterol (trade name: Phytosterol-F, manufactured by Tama Seikagaku Co., Ltd.) was dispersed in a transparent state to obtain an oil phase. The oil phase was gradually added to the aqueous phase, mixed and stirred, and then homogenized with a high-pressure homogenizer to obtain 1000 g of a uniform plant sterol-containing water-soluble composition having an HLB value of 13.

Example 2 115 g of decaglycerin monolaurate of Example 1 was replaced with decaglycerin monomyristate [trade name: Sunsoft Q]
-14S, manufactured by Taiyo Chemical Co., Ltd., HLB 14.5] 115
g except that the same operation as in Example 1 was performed.
A homogeneous plant sterol-containing water-soluble composition having an HLB value of 12 was obtained.

Comparative Example 1 115 g of decaglycerin monolaurate of Example 1 was replaced with pentaglycerin monooleate (trade name: Sunsoft A)
-171E, manufactured by Taiyo Kagaku Co., Ltd., HLB13] 115 g
The same operation as in Example 1 was performed except that
A uniform plant sterol-containing water-soluble composition having an LB value of 11 was obtained.

Comparative Example 2 115 g of decaglycerin monolaurate of Example 1 was replaced with decaglycerin monostearate [trade name: Poem J-0]
081H manufactured by Riken Vitamin Co., Ltd., HLB12] 115
g except that the same operation as in Example 1 was performed.
A uniform plant sterol-containing water-soluble composition having an HLB value of 10 was obtained.

Example 3 The same operation as in Example 1 was carried out except that the total amount of 535 g of water and glucose fructose liquid sugar of Example 1 was changed to 535 g of water, and a uniform plant sterol having an HLB value of 13 was contained. A water-soluble composition was obtained.

Comparative Example 3 A total of 510 g of decaglycerin monolaurate and fructose fructose liquid sugar of Example 1 was changed to 510 g of fructose fructose liquid sugar [trade name: KM-F55 manufactured by Nippon Shokuhin Kogyo KK]. The same operation as in Example 1 was performed,
A water-insoluble gel-like composition was obtained, and a uniform and stable water-soluble phytosterol-containing composition could not be obtained. (The HLB value of the composition is 3)

Comparative Example 4 In place of decaglycerin monolaurate in Example 1, an enzyme-degraded soybean lecithin [San Lecithin A Taiyo Kagaku Co., Ltd.
And HLB12] was used in the same manner as in Example 1 except that 115 g of a plant sterol-containing water-soluble composition having an HLB value of 10 was obtained.

Example 4 100 g of decaglycerin monomyristate [trade name: Sunsoft Q-14S, manufactured by Taiyo Chemical Co., Ltd.], triglycerin monomyristate [trade name: Sunsoft A-141]
C, manufactured by Taiyo Kagaku Co., Ltd., HLB12] 20 g, water 140
g and sorbitol (trade name: Sorbitol C, manufactured by Niken Kagaku Co., Ltd.) (390 g) were placed in a container, heated and completely dissolved to obtain an aqueous phase. Separately, glycerin lactate monostearate [trade name: Sunsoft No. 661AS
Taiyo Kagaku KK, HLB7.5] 25 g, corn oil 2
95 g of vegetable sterol [trade name: phytosterol-F manufactured by Tama Biochemical Co., Ltd.] 30 with heating and stirring
g was dispersed in a transparent state to obtain an oil phase. The oil phase was gradually added to the aqueous phase, mixed and stirred, and then homogenized with a high-pressure homogenizer to obtain an HLB value of 12
Of a homogeneous plant sterol-containing water-soluble composition was obtained.

Comparative Example 5 A total of 120 g of decaglycerin monomyristate and triglycerin monomyristate of Example 4 was used as a sucrose stearate [trade name: Ryoto Sugar Ester S-970, manufactured by Mitsubishi Chemical Foods Corporation, HLB9] ]
Except that the amount was changed to 120 g, the same operation as in Example 4 was performed, but the mixture was separated immediately after preparation, and a homogeneous plant sterol-containing water-soluble composition could not be obtained. (HL of composition
B value is 8)

Example 5 Decaglycerin monolaurate [trade name: Poem J-0]
021 manufactured by Riken Vitamin Co., Ltd., HLB16] 120
g, 240 g of water, and 290 g of glycerin [trade name: glycerin for food addition, manufactured by Nippon Oil & Fats Co., Ltd.] were placed in a container, heated and completely dissolved to obtain an aqueous phase. Separately, sucrose palmitate [trade name: Ryoto Sugar Ester P-170, manufactured by Mitsubishi Chemical Foods Corporation, HLB
1] 25 g, vitamin A oil [trade name: vitamin A oil (vitamin A 1,000,000 IU / g), manufactured by Kanematsu Foods Co., Ltd.] 3.0 g, vitamin E oil [trade name: RIKEN E Oil 805 ( d-α-vitamin E content 400 mg /
g) RIKEN Vitamin Co., Ltd.] 267 g of rapeseed oil to which 30 g was added was heated, and plant sterol [trade name: phytosterol-F Tama Biochemical Co., Ltd.] 2 with stirring
5 g was dispersed in a transparent state to obtain an oil phase. The oil phase was gradually added to the aqueous phase, mixed and stirred, and then homogenized with a high-pressure homogenizer to obtain an HLB value of 1
Thus, a water-soluble composition containing 3 uniform fat-soluble vitamins and plant sterol was obtained.

Comparative Example 6 The same operation as in Example 5 was carried out except that the total of 410 g of decaglycerin monolaurate and glycerin in Example 5 was changed to 410 g of glycerin (trade name: edible glycerin, manufactured by NOF Corporation). Was carried out to obtain a water-soluble composition containing a uniform fat-soluble vitamin having an HLB value of 1 and a plant sterol.

Comparative Example 7 The procedure of Example 5 was repeated, except that 120 g of decaglycerin monolaurate of Example 5 was replaced with 120 g of enzymatically degraded soybean lecithin [Sun Lecithin A, manufactured by Taiyo Kagaku KK, HLB12]. A water-soluble composition containing fat-soluble vitamins having an HLB value of 10 and plant sterol was obtained.

Comparative Example 8 In place of 120 g of decaglycerin monolaurate in Example 5, sucrose stearic acid ester [trade name: Ryoto Sugar Ester S-970 Mitsubishi Chemical Foods Corporation]
And HLB11] was used in the same manner as in Example 5 except that 120 g of a fat-soluble vitamin having an HLB value of 9 and a plant sterol were obtained.

Example 6 A beverage having the composition shown in Table 4 was prepared using the plant sterol-containing water-soluble composition (HLB value 13) of Example 5,
L-volume bottles were filled and sealed. This beverage was sterilized by heating at 90 ° C. for 15 minutes to obtain a beverage. After the beverage thus obtained was stored at 40 ° C. for 30 days, the dispersion stability was evaluated by the following storage test. As a result, no crystal precipitation of plant sterol was observed, and the dispersion state was stable.

Comparative Example 9 Example 6 was repeated except that the plant sterol-containing water-soluble composition of Example 5 was replaced with the plant sterol-containing water-soluble composition of Comparative Example 7 (HLB value: 10) in the ingredients shown in Table 4. A beverage was obtained in the same manner as described above. After the beverage thus obtained was stored at 40 ° C. for 30 days, dispersion stability was evaluated. As a result, precipitation of plant sterol crystals was observed, and the dispersion state was unstable.

Using the plant sterol-containing water-soluble composition or the water-soluble composition containing fat-soluble vitamins and plant sterol obtained in Examples 1 to 6 and Comparative Examples 1 to 9, a heat resistance test was carried out by the following method. An acid / heat resistance test, a salt / heat resistance test, and a 40 ° C storage test of these test samples were performed. <Heat resistance test, acid / heat resistance test, salt / heat resistance test, and
40 ° C. Storage Test of Those Test Samples> Storage Test Immediately after preparation and after storage at 40 ° C. for 30 days, the appearance of the water-soluble composition containing plant sterols or fat-soluble vitamins and the water-soluble composition containing plant sterols was visually evaluated, and the results are shown in the table. In the table, ○ indicates good stability, Δ indicates slightly poor stability, X indicates poor stability, floating / sedimentation occurred, * indicates separation immediately after preparation or storage, ** indicates gel immediately after preparation or storage. , Respectively.

2. Heat resistance test Plant-sterol-containing water-soluble composition or fat-soluble vitamins 1% of plant-sterol-containing water-soluble composition is added to purified water, and at room temperature, plant-sterol-containing water-soluble composition or fat-soluble vitamins and plant-sterol-containing water-soluble. The appearance state of the aqueous solution of the hydrophilic composition was visually evaluated. Further, in order to confirm the heat resistance of the aqueous solution, the aqueous solution was immersed in hot water and heated for 30 minutes from the time when the temperature of the aqueous solution reached 90 ° C. After allowing these aqueous solutions to cool at room temperature, the appearance was visually evaluated, and the heat resistance of the aqueous solutions of the plant sterol-containing water-soluble composition or the fat-soluble vitamins plant sterol-containing water-soluble composition was confirmed. The results of each visual evaluation are shown in the table.

3. Acid / Heat Resistance Test 1% of a plant sterol-containing water-soluble composition or a fat-soluble vitamin and a plant sterol-containing water-soluble composition are added to purified water adjusted to a pH of 3 or less with citric acid, and the appearance at room temperature is determined. It evaluated visually and confirmed the acid resistance of the plant sterol-containing water-soluble composition or the fat-soluble vitamins plant sterol-containing water-soluble composition. Further, in order to confirm the heat resistance under the acidic condition, the acidic aqueous solution containing the composition was subjected to a water bath and a heat treatment was performed for 30 minutes from the time when the liquid temperature reached 90 ° C. After allowing these composition-containing acidic aqueous solutions to cool at room temperature, the appearance was visually evaluated, and the acid resistance and heat resistance of the plant sterol-containing water-soluble composition or the fat-soluble vitamins and the plant sterol-containing water-soluble composition were evaluated. confirmed. The results of each visual evaluation are shown in the table. In the table, ○ indicates good stability, and × indicates floating and sediment generation.

4. Salt resistance / heat resistance test Similarly, 1% of a plant sterol-containing water-soluble composition or a fat-soluble vitamin and a plant sterol-containing water-soluble composition are added to purified water containing 5% of salt, and the appearance at room temperature is visually observed. Evaluation was performed to confirm the salt resistance of the plant sterol-containing water-soluble composition or fat-soluble vitamins and the plant sterol-containing water-soluble composition. Further, in order to confirm the heat resistance under the high salt concentration condition, the salt water containing the composition was subjected to hot water bath, and a heat treatment was performed for 30 minutes from the time when the liquid temperature reached 90 ° C. The appearance of these compositions-containing saline was visually evaluated, and the salt resistance and heat resistance of the plant sterol-containing water-soluble composition or the fat-soluble vitamins and the plant sterol-containing water-soluble composition were confirmed. The results of each visual evaluation are shown in the table. In the table, ○ indicates good stability, × indicates floating,
Each represents the generation of a precipitate.

The above heat resistance test, acid resistance / heat resistance test, salt resistance
The aqueous solution of the plant sterol-containing water-soluble composition or the fat-soluble vitamins and the plant sterol-containing water-soluble composition used in the heat resistance test was stored in a thermostat at 40 ° C for 30 days, and the appearance of each was visually evaluated. . The results of each visual evaluation are shown in the table. In the table, ○ indicates good stability,
× represents floating and sediment generation, respectively. Example 1 above
Tables 1, 2 and 3 show the composition and results of Comparative Examples 1 to 8. Table 4 shows the composition and results of the beverages of Example 6 and Comparative Example 9.

[0048]

[Table 1]

[0049]

[Table 2]

[0050]

[Table 3]

[0051]

[Table 4]

The abbreviations used in the table are as follows. G10 mono C12; decaglycerin monolaurate, G10 mono C14; decaglycerin monomyristate, G5 mono C18: 1; pentaglycerin monooleate, G10 mono C18; decaglycerin monostearate, G3 mono C14; triglycerin monomilli State.

From the above results, it can be seen that the systems in which the plant sterols of the present invention of Examples 1 to 5 are blended exhibit superior stability, acid resistance and the like as compared with the systems of Comparative Examples 1 to 8. Further, Example 6 in which the water-soluble composition of the present invention was blended into a beverage was 40 times less than Comparative Example 9 of the beverage using Comparative Example 7.
It can be seen that the stability at 30 ° C. × 30 days is excellent.

Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat II (reference) A23L 2/38 A23L 2/00 F

Claims (7)

[Claims] 1. A method comprising: (A) plant sterol, (B) glycerin fatty acid ester, organic acid monoglyceride, polyglycerin fatty acid ester, propylene glycol fatty acid ester, polyglycerin condensed ricinoleate, sorbitan fatty acid ester, sucrose fatty acid ester and lecithin. One or more lipophilic emulsifiers selected from the group consisting of (C) edible oils, (D) at least one hydrophilic polyglycerin fatty acid ester, and (E)
A plant sterol-containing water-soluble composition comprising water and having an HLB value of an emulsifier of 12 or more in the entire system. (A) 0.05 to 50% by weight of a plant sterol, (B) 0.01 to 50% by weight of a lipophilic emulsifier, (C) 0.01 to 50% by weight of edible oils, and (D) hydrophilicity. 0.5 to 50% by weight of polyglycerol fatty acid ester of
HLB value of emulsifier in the whole system is 1
The plant sterol-containing water-soluble composition according to claim 1, which is 2 or more. (3) Vitamin A, Vitamin D,
1 selected from the group consisting of vitamin E and vitamin K
3. A fat-soluble vitamin and a plant sterol-containing water-soluble composition according to claim 1 or 2, characterized in that it contains one or more fat-soluble vitamins. (F) The fat-soluble vitamin content is 0.0
The plant sterol-containing water-soluble composition according to claim 3, which is 1 to 72.5% by weight. 5. A method for producing a plant sterol-containing water-soluble composition according to claim 1, wherein the oil phase comprises (A) a plant sterol, (B) a lipophilic emulsifier, and (C) an edible oil. And a water phase comprising (D) a hydrophilic polyglycerin fatty acid ester and (E) water, and a homogenizing treatment is carried out. The water-soluble composition containing a plant sterol having an HLB value of 12 or more in the entire mixture is 12 or more. Method of manufacturing a product. 6. The method for producing a water-soluble composition containing a plant sterol according to claim 3 or 4, wherein (A) a plant sterol, (B) a lipophilic emulsifier, (C) an edible oil and (F). An oil phase containing fat-soluble vitamins and an aqueous phase consisting of (D) a hydrophilic polyglycerin fatty acid ester and (E) water are blended and homogenized, and the HLB value of the emulsifier in the whole mixture becomes 12 A method for producing a plant sterol-containing water-soluble composition as described above. 7. A food or drink comprising the plant sterol-containing water-soluble composition according to any one of claims 1 to 4.