JP2002277357A - Blood filter, and blood collecting implement - Google Patents

Abstract

PROBLEM TO BE SOLVED: To precisely separation-recover a sufficient amount of serum component without widening a filtration area. SOLUTION: The first filter 31 having a property capable of permeating the serum component in an inflow blood at a velocity faster than that of a hemocyte component, and the second filter 32 having a property capable of permeating only the serum component but incapable of permeating the hemocyte component are held inside a main body tube 20 in a serial condition, in this blood filter F of the present invention, and blood is made to flow in order of the first filter 31 and the second filter 32 to recover the serum component passing through the second filter 32. The membrane-like second filter 32 is assembled inside the main body tube 20 under the condition where it is held inside a filter holding member 38, to be protected. This blood collecting implement of the present invention is constituted to allow the filtration to be carried out at the place where the blood is collected, using the blood filter F.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood filter for collecting serum components in blood and a blood collector using the filter.

[0002]

2. Description of the Related Art Conventionally, various means have been developed as means for filtering blood and collecting serum components thereof. For example, 1) Japanese Patent No. 3015854 discloses that
A blood collection device using a membrane filter that captures blood cell components and allows only plasma components to pass through is disclosed.
In Japanese Patent Publication No. 1-180876, blood is passed through a filter made of ultrafine fibers, and a serum component is separated from blood by utilizing a difference between a moving speed of a blood cell component and a moving speed of a serum component in the filter. A method is disclosed.

[0003]

The above-mentioned Patent No. 30158 is disclosed.
The filter disclosed in Japanese Patent Publication No. 54 captures blood cell components and interrupts the flow of blood cells. Therefore, if the filtration area is small, clogging of blood cell components occurs in a relatively short time. In other words, in order to perform a sufficient amount of filtration, the size of the filter is increased, or the filter area is reduced by arranging the filter in a V-shape or a cylindrical shape as shown in the above-mentioned publication. It is necessary to make efforts to earn money, and it is difficult to reduce the size and the structure of the filter.

On the other hand, the filter disclosed in Japanese Patent Application Laid-Open No. H11-180876 is made of a fibrous body, and does not completely block blood cell components but transmits blood cell components with a delay after a serum component. Although the above-described inconvenience due to clogging is unlikely to occur, if blood is introduced and left for a relatively long time, blood cell components in addition to serum components will flow out. There is a disadvantage that it is difficult to obtain accuracy.

[0005] Japanese Patent No. 3015854 discloses that a plurality of kinds of porous bodies are laminated so that the pore diameter becomes smaller from the upstream side to the downstream side so that clogging of blood cells is prevented. However, such a configuration in which a plurality of types of porous bodies are stacked is complicated and tends to increase the cost. Also, it is difficult to set the pore diameter of each porous body.

Accordingly, the present inventors have developed a first filter made of a fibrous body and having a property of transmitting the serum component in the inflowing blood at a higher speed than the blood cell component, and a blood cell component which allows only the serum component to pass therethrough. Arranged in series with a second filter made of a porous membrane that is not permeable, and conceived of collecting blood serum components that passed through the second filter by flowing blood in the order of the first filter and the second filter. However, the second filter made of the porous membrane is weak to an external force, and may be broken by a strong compressive force to lose a filtering function.

[0007]

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems, and is directed to a blood filter for separating and collecting at least serum components in blood from blood cell components. A first filter made of a fibrous body and having a property of allowing serum components in the inflowing blood to pass through at a higher speed than blood cell components; and a membrane-shaped second filter having a property of allowing serum components to pass but not allowing blood cell components to pass. Filter, and the first filter and the second filter are held in series, and a blood introduction part for introducing blood into the first filter and a serum component that has passed through the second filter are discharged. And a retainer having a discharge portion that performs
The retainer body filled with the first filter and the second filter are held in a state of being sandwiched between the upstream side and the downstream side, and blood is collected at an upstream portion and a downstream portion of the filter. And a filter holding member having a flow portion for flowing, and the filter holding member is incorporated in the holder main body on the downstream side of the first filter.

[0008] In this configuration, in the first filter into which blood is first introduced, there is a difference between the permeation speed of the serum component and the permeation speed of the blood cell component. Out of the filter of
Also pass through. Therefore, it is possible to collect a serum component free of blood cell components from the second filter.

Thereafter, the blood cell component starts to flow out of the first filter with a delay, but the blood cell component is captured by the second filter and cannot pass through the same filter. And the separation accuracy is kept high. As the time elapses, clogging of the second filter with the blood cell component occurs. However, at that time, since the serum component has already been sufficiently collected, there is no problem even if the clogging occurs.

[0010] Further, the retainer retains the second filter in a state sandwiching the second filter from the upstream side and the downstream side thereof, and
Since it includes a filter holding member having a circulation part for circulating blood in the upstream part and the downstream part of the filter, the effective filtration while effectively protecting the membrane-like second filter, which is disadvantageous in strength, is performed. Function can be secured.

The second filter only needs to have a property of transmitting at least a serum component and not a blood cell component, and the other components may be either permeable or non-permeable. For example, it may transmit not only serum components but also plasma components.

Although the first filter and the filter holding member may be separated from each other, the second filter is effectively protected by the filter holding member even when both are brought into close contact with each other.
For example, it is also possible to adopt a configuration in which the first filter is filled in a compressed state in a space surrounded by a retainer body and a filter retaining member fixed in the retainer. The two filters can be housed in the holder in a compact state, while the second filter is effectively protected by the filter holding member.

In the present invention, as described above, a sufficient serum component can be collected before the second filter is clogged. Therefore, it is necessary to increase the filtration area of the second filter. For example, the filter may be held in the filter holding member in a flat state, thereby making it possible to reduce the size and the structure of the entire filter.

In this case, the filter holding member sandwiches only the peripheral portion of the second filter, and a gap is secured between the front and back surfaces of the second filter excluding the peripheral portion and the filter holding member. With such a configuration, it is possible to greatly reduce the strength load on the second filter and maintain the life of the second filter for a long time.

Further, the present invention comprises the blood filter and a blood collecting section which is detachably connected to a discharge section of the holder in an airtight state and detachably connected thereto. The blood collecting needle is inserted into the opening formed in the blood collecting portion so as to close the opening in an airtight state, and a negative pressure can be formed inside the blood collecting portion and the retainer. It is configured as follows.

According to this blood collecting apparatus, blood can be collected and separated efficiently, for example, in the following manner.

The pressure inside the holder and the blood collection unit is reduced by piercing the suction tube into the stopper of the holder. This decompression may be performed at a stage before distribution of the blood collection device. That is, the blood collection device may be provided after the pressure in the tube is reduced by the manufacturer. That is, the present invention can be provided as a blood collection device in which a negative pressure is formed inside the retainer and the blood collection unit with the blood collection unit connected to the retainer.

With one end of a blood collection needle pierced into a blood vessel, the other end of the needle is inserted through a stopper. At this time, the blood automatically flows into the upstream pipe due to the negative pressure in the retainer.
Furthermore, when the atmospheric pressure is introduced into the holder by opening the stopper, a differential pressure is generated between the upstream side and the downstream side of the two filters, and the blood pressure causes the blood to flow through the first filter and the second filter.
And the serum component that has passed through the second filter flows into the blood collection unit from the outlet of the holder,
Will be stored.

After the separation is completed, the blood collecting part is separated from the holder. This blood collection unit can be used for a test as a test tube, for example.

Here, as a structure for detachably attaching the blood collecting portion to the holder, for example, a screw portion which is screwed to the holder and the blood collecting portion may be formed. A collection container that opens to the storage container, and a rubber stopper attached to the opening of the collection container so as to close the opening,
If the discharge portion of the blood filter has a needle-like tube portion that penetrates the rubber stopper of the blood collecting portion and enters the collection container, the needle-like tube portion is pierced into the rubber stopper. By merely penetrating, the inside of the collection container and the inside of the retainer can be communicated while maintaining the sealed state.

Further, by integrally forming the needle-like tube portion with the filter holding member, the number of parts can be reduced and the structure can be further simplified.

[0022]

FIG. 1 shows a first embodiment of the present invention.
A description will be given based on FIG.

The illustrated blood collecting apparatus includes a blood filter F and a blood collecting section C.

The blood collection section C includes a collection tube (collection container) 10
And a rubber stopper 12. The collection tube 10 has a test tube shape that opens only in one direction. The rubber stopper 12
A small diameter portion 14 slightly larger in diameter than the opening of the collecting pipe 10
And a large-diameter portion 16 having a diameter larger than the small-diameter portion 14 and slightly larger than the inner diameter of the large-diameter portion 24 in the main body tube 20 described later. Recovery pipe 1
By press-fitting into the opening 0, the opening is closed in an airtight state. As shown in FIG. 2, the axial center portion of the rubber plug 12 has a thin portion 18 which is thinner than other portions by notching the upper and lower ends.

The blood filter F includes a main tube 2 having both ends opened.
It has 0. The upstream side (upper side in FIG. 1) of the main body tube 20 in the filtration direction is a small-diameter portion 22, and the downstream side is a large-diameter portion 24 into which the large-diameter portion 16 of the rubber plug 12 can be press-fitted. 22 and the large diameter portion 24 are partitioned by an inner wall 26. The inner wall 26 is provided with a through hole 27 as shown in FIGS. 2 and 3, through which blood can flow from the small diameter portion 22 to the large diameter portion 24.

Opening on the upstream side of the main body tube 20 (opening on the small diameter portion side)
Is closed by a stopper 28. This stopper 28 is also integrally formed of rubber similarly to the rubber stopper 12, and its axial center portion is a thin portion 29 which is thinner than the other portions, and is pressed into the opening to close the opening. It is closed in an airtight state.

In the large diameter portion 24, a first filter 31 and a second filter 32 are stored in a state of being arranged in series from the side closer to the inner wall 26 (ie, from the upstream side).

The first filter 31 is an aggregate of a large number of small-diameter fibers.
4 is loaded. The fiber diameter and the degree of compression (density) may be set so as to obtain such a property that a blood cell component is transmitted behind the serum component as described later.
By using the one compressed to 5 g / cm ² ,
It has been experimentally confirmed that the above properties can be obtained. In addition, the filter disclosed in JP-A-11-180876 can be used as it is.

The second filter 32 may be of any type as long as it has a property that allows serum components in blood to pass through but does not allow blood cell components to pass through. As a specific example, a general filtration membrane (membrane filter) may be used to capture blood cells by utilizing the function of its molecular sieve, or an ordinary filter paper having a large pore diameter may contain anti-blood cell antibodies. Alternatively, the blood cell component may be captured using specific binding between the antibody and the blood cell. In addition, a porous body or the like that can block passage of blood cell components is applicable.

The second filter 32 has a flat film shape in the illustrated example, and is held by a filter holding member 38 while being sandwiched from both sides. The filter holding member 38 and the main tube 20 are integrated with the filter holding member 38 in the large-diameter portion 24.
Thus, the holder of the blood filter F is configured. That is, the main body tube 20 forms the main body of the retainer.

The filter holding member 38 includes a main body 34,
It consists of a filter cover 36.

The main body 34 is shaped like a saucer having a concave portion that opens on the first filter 31 side (upstream side), and the second filter 32 is laid on the bottom thereof. More specifically, a concave portion 43 slightly lower than the peripheral edge portion is formed at the center of the main body portion 34, and the main body portion 3 has a depth corresponding to the depth of the concave portion 43.
A small gap is secured between the bottom surface of the fourth filter 4 and the lower surface of the central portion of the second filter 32.

The filter cover 36 is mounted on the concave portion of the main body 34 so as to cover the second filter 32 laid on the main body 34 from the upstream side. Are formed in the thickness direction. Step portions 46 and 48 are formed on the outer peripheral portion of the filter cover 36 and the inner peripheral portion of the concave portion of the main body portion 34, respectively, in contact with each other in the axial direction. And direct contact with the central portion of the second filter 32 are prevented. That is, a gap is provided between the filter cover 36 and the upper surface of the second filter 32.

The specific means for fixing the second filter 32 to the filter holding member 38 does not matter. For example, as shown in FIG. 5 (a), a second filter 32 may be laid on the main body portion 34, and then a filter holding portion 35 for closing the peripheral portion thereof may be secondarily formed on the main body portion 34. Or, the lower surface of the filter cover 36 shown in FIG. 6B (the surface on the side facing the second filter 32).
A ridge 49 for ultrasonic welding may be formed in advance, and the periphery of the filter 32 may be fixed by the welding.

In any case, only the peripheral portion of the second filter 32 is sandwiched by the filter holding member 38, and the other filter portion (central portion) and the main body portion 3 are held.
If a gap is secured between the second filter 32 and the filter cover 36, the strength load on the second filter 32 can be reduced and its good filtration function can be secured.

The needle-like tube portion 4 is located downstream from the main body portion 34.
The needle-like tube portion 40 has a through-hole 42 at the axial center thereof, and has a sharp needle-like tip. The through-hole 42 of the needle-like tube portion 40 allows the second filter 3
Two downstream sides are communicated outward.

Next, the procedure for assembling and using the blood collector will be described.

The second filter 32 is incorporated in the filter holding member 38. On the other hand, the collection pipe 10 and the main pipe 20
Are fitted with stoppers 12, 28, respectively.

As shown in FIG. 6, the large diameter portion 2 of the main body tube 20
First, the fibrous body constituting the first filter 31 is put into the filter 4, and thereafter, the filter holding member 38 is pushed in to compress the fibrous body. Then, the holding member 38 is pushed to a position where the filter holding member 38 gets over the projection 23 formed in advance on the inner surface of the large diameter portion 24. When the pushing is completed, the filter holding member 38 is locked by the projection 23 and fixed in the main body tube 20.

The large-diameter portion 16 of the rubber stopper 12 attached to the collection tube 10 is pressed into the large-diameter portion 24 of the main body tube 20, and the needle-shaped filter holding member 38 is inserted into the thin portion 18 of the rubber stopper 12 from outside. The pipe portion 40 is pierced, and the thin portion 18
Through. As a result, the rubber stopper 12 closes the opening of the large-diameter portion 24 in an airtight state, and the needle-like tube portion 40
The inside of the collection pipe 10 and the inside of the filter holding member 38 communicate with each other.

For example, a suction tube is pierced into the stopper 28 of the main body tube 20 so as to face the collection tube 10, and the air in the main body tube 20 and the collection tube 10 is suctioned, so that the internal space of the whole blood collection device is negatively charged. Pressure. The specific pressure value may be appropriately set according to the specifications of the blood collection device. This decompression is
The procedure may be performed by the manufacturer before distribution of the blood collection device, or may be performed by a user who has been provided with the blood collection device. Alternatively, a negative pressure may be applied only to the inside of the recovery pipe 10 in advance, and the main pipe 20 side may be set to a negative pressure when the needle-shaped pipe section 40 is pierced.

<Blood Collection> The distal end (upper end in the figure) of the blood collection needle 50 as shown by the two-dot chain line in FIG. 1 is pierced into the blood vessel of the subject, and the rear end (lower end in the figure) is a thin portion 29 of the stopper 28. And penetrate it. At this time, the blood collection needle 50 is fixed to the cap 52 as shown by the two-dot chain line in advance, and the cap 52 is attached to the small diameter portion 22 of the main body tube 20 so that the blood collection needle 50 By penetrating the thin portion 29, the blood sampling operation becomes easier.

Through the penetration of the blood collection needle 50, the blood in the blood vessel of the subject is drawn into the small-diameter portion 22 of the main tube 20 by the action of the negative pressure in the main tube 20. Further, when the stopper 28 is opened (for example, the stopper 28 is removed from the body tube 20), and the atmospheric pressure is introduced into the body tube 20, the pressure in the small diameter portion 22 becomes higher than that in the recovery tube 10. Therefore, the blood tries to flow into the collection tube 10 from the through hole 27 of the inner wall 26 through the first filter 32 and the second filter 34 in the filter holding member 38 due to the pressure difference.

Here, in the first filter 32, there is a difference between the permeation speed of the serum component and the permeation speed of the blood cell component. Spill out of. The serum component passes through the through hole 44 of the filter cover 36 and further passes through the second
The filter 32 also passes through the through-hole 42 of the needle-shaped tube portion 40 and flows into and is stored in the collection tube 10.

Thereafter, the blood cell component starts to flow out of the first filter with a delay, but this blood cell component is captured by the second filter 32 and cannot pass through the same filter 32.
The blood cell component is prevented from being mixed into the serum component in the blood collection unit 10, and the separation accuracy is kept high. When the time further elapses, clogging of the second filter 32 due to the blood cell component occurs. At that time, since the serum component is sufficiently collected in the collection tube 10, even if the clogging occurs, there is no problem. No problem.

In the present invention, it does not matter whether or not components other than the blood cell component and the serum component are captured by the filter. For example, in this embodiment, fibrinogen which does not belong to any of the blood cell component and the serum component adheres to the fibers constituting the first filter 31 in this embodiment, and therefore, the collection tube 1
It hardly mixes with the serum components in 0.

After the collection of the serum component in the collection tube 10 is completed as described above, the blood collection part C may be withdrawn from the main tube 20 and the stopper 12 may be further removed. Since the serum components are collected in the collection tube 10, the collection tube 10 can be used as a test tube for inspection.

FIG. 7 shows a second embodiment of the present invention.
In the above-described embodiment, the needle holding section 4 is attached to the filter holding member 38.
0 was formed integrally, but here, the cap 56
The blood collection tubular needle 54 provided with is used as a needle-like tubular portion.

More specifically, the downstream side of the large diameter portion 24 of the main body tube 20 is closed except for the small diameter tube 25.
Both filters 31 and 32 are confined inside. The second filter 32 in the form of a membrane is sandwiched from both sides by a filter-like filter holding member 39 (having liquid permeability),
Is protected. Then, a cap 56 of the tubular needle 54 is attached to the small-diameter tube 25 to form a discharge unit. At the downstream end of the large diameter portion 24,
A hood 2 for security that covers the tubular needle 54 from the radial outside.
4a are formed. The hood 24a can be omitted as appropriate.

In this blood collector, the hood 24a
The tubular needle 54 is pierced into the rubber stopper 12 while the rubber stopper 12 of the collection tube 10 is inserted therein, so that the inside of the collection tube 10 is communicated with the main body tube 20 through the tubular needle 54, and blood is collected in the same manner as described above. Can be performed.

According to the present invention, in addition to connecting the collecting pipe 10 and the main body pipe 20 with the combination of the needle-shaped pipe section and the rubber stopper as described above, for example, a threaded portion screwed to the main body pipe 20 and the collecting pipe 10 is used. Is formed, it is possible to connect the recovery pipe 10 to the main pipe 20 in an airtight state.

Further, in the present invention, the main body tube 20 and the collecting tube 10 shown in FIG. 1 are integrated, and after collecting the serum component, the portion downstream of the second filter 32 is cut off to collect the collected serum component. May be taken out. Again,
The needle-like tube portion and the stopper 12 become unnecessary, and the second filter 3
The downstream side of 2 directly corresponds to the “discharge section” of the blood filter according to the present invention.

FIG. 8 shows a third embodiment. In the above-described embodiment, the blood filter F is integrated with the blood collection device. However, in the third embodiment, the blood filter F is made independent and the blood collected by the syringe 70 is filtered again. It is to be filtered by the vessel F.

More specifically, the holding tube (holding body) 60 of the blood filter F is a first housing for housing the first filter 31.
A filter housing 61, a second filter housing 62 for housing the second filter 32 and the filter holding member 39 sandwiching the second filter 32, a communication pipe 64 connecting the two housings 61, 62, and a second filter. It has a discharge pipe section 66 provided on the downstream side of the storage section 62 and a blood introduction pipe section 68 provided on the upstream side of the first filter storage section 61. The cap 56 of the tubular needle 54 is attached to the discharge pipe 66, and the distal end of the cylinder 72 is fitted into the blood introduction pipe 68 with the injection needle removed from the cylinder 72 of the syringe 70. I can do it.

Even in such a configuration, the syringe 7
After the blood is collected at 0, the tip of the cylinder 72 of the syringe 70 is inserted into the blood introduction tube 68 of the holder 60, and a needle is inserted into the stopper 12 of the blood collection unit C (FIG. 7) whose inside is at a negative pressure. 5
If the inside of the retainer 60 is also made into a negative pressure by piercing the syringe 4, the blood in the syringe 70 is filtered by the negative pressure by the filters 61 and 62.
Thus, filtration can be performed in the same manner as in the first embodiment, and the blood can be collected in the blood collecting section C through the blood introducing tube 68. In addition to using the negative pressure, the blood in the syringe 70 can be sent into the blood filter F by operating the piston 74 of the syringe 70.

[0056]

As described above, according to the present invention, there is provided the first type having the property that there is a difference between the permeation speed of the serum component and the permeation speed of the blood cell component.
And a second filter having a property of allowing the serum component to pass therethrough but not allowing the blood cell component to pass therethrough is held in a holder in a holding state, and the serum component that has passed through the second filter is collected. Therefore, a sufficient amount of serum components can be separated and recovered with high accuracy with a simple configuration without particularly increasing the filtration area of the second filter. Moreover, since the second filter in the form of a membrane is incorporated in the holder body while being held in the filter holding member, the second filter can be prevented from being crushed or the like, and its favorable filtering function can be secured. There is.

[Brief description of the drawings]

FIG. 1 is a cross-sectional front view of a blood collector according to a first embodiment of the present invention.

FIG. 2 is a sectional front view showing a main part of the blood collecting device.

FIG. 3 is a sectional view taken along line AA of FIG. 2;

FIG. 4A is a plan view of a filter cover of a filter holding member provided in the blood collecting device, and FIG. 4B is a partial cross-sectional front view thereof.

FIG. 5A is a cross-sectional view showing an example in which the second filter is fixed by performing secondary molding on the main body of the filter holding member, and FIG. 5B is a view in which a projection for ultrasonic welding is formed. FIG. 4 is a partial cross-sectional front view showing an example of a filter cover.

FIG. 6 is a partially sectional front view showing a step of loading a filter into a main tube of the blood collecting device.

FIG. 7 is a cross-sectional front view of a blood collector according to a second embodiment of the present invention.

FIG. 8 is a sectional front view of a blood filter according to a third embodiment of the present invention.

[Explanation of symbols]

 F Blood filter C Blood collection unit 10 Collection tube (collection container) 12 Rubber stopper 20 Main tube (constituting retainer) 28 Plug 31 First filter 32 Second filter 38, 39 Filter holding member 40 Needle tube 54 tubular needle (needle tube part) 60 holding tube (main body of holder)

Continued on the front page (72) Motoki Kyo, 2-1-1 Katata, Otsu-shi, Shiga Prefecture Inside Toyobo Co., Ltd. (72) Inventor Hirofumi Ogawa 2-2-2 Dojimahama, Kita-ku, Osaka-shi, Osaka Toyo Spinning Co., Ltd. F-term (reference) 2G045 AA01 BA08 BA10 BB06 CA25 CA26 FA29 HA06 HA13 HA14 HB02 2G052 AA30 AD06 AD26 AD46 BA13 DA02 DA12 DA13 DA25 EA02 EA03 JA21 4C038 TA01 TA10 UA06 UB07 UB10

Claims (9)

[Claims] 1. A blood filter for separating and collecting at least serum components in blood from blood cell components, the filter comprising a fibrous body, and permeating serum components in the inflowing blood at a higher speed than blood cell components. A first filter having the property of causing
A membrane-like second filter having a property of allowing a serum component to pass therethrough and not allowing a blood cell component to pass therethrough, holding the first filter and the second filter in series, and applying blood to the first filter. A retainer having a blood introduction part for introduction and a discharge part for discharging the serum component transmitted through the second filter, the retainer includes a retainer main body filled with the first filter, A filter holding member that holds the second filter in a state of being sandwiched between the upstream side and the downstream side, and has a flow part that allows blood to flow in the upstream part and the downstream part of the filter; A blood filter, wherein a filter holding member is incorporated in the holder main body on the downstream side of the first filter. 2. The blood filter according to claim 1, wherein the second filter has a property of transmitting plasma components. 3. The blood filter according to claim 1, wherein the first filter is compressed in a space surrounded by the holder main body and a filter holding member fixed in the holder. A blood filter characterized by being filled. 4. The blood filter according to claim 1, wherein the second filter is held in the filter holding member in a flat state. 5. The blood filter according to claim 4, wherein the filter holding member sandwiches only a peripheral edge of the second filter, and the front and rear surfaces of the second filter excluding the peripheral edge. A blood filter, wherein a gap is secured between the filter and a filter holding member. 6. The blood filter according to claim 1, further comprising: a blood collection unit that is detachably connected to a discharge unit of the holder in an airtight state. The introduction unit is configured such that a stopper through which a blood collection needle can be inserted is attached to an opening formed in the retainer so as to close the opening in an airtight state, and is provided inside the blood collection unit and the retainer. A blood collection device configured to be capable of forming a negative pressure. 7. The blood collection device according to claim 6, wherein a negative pressure is formed inside the holder and the blood collection unit in a state where the blood collection unit is connected to the holder. vessel. 8. The blood collection device according to claim 6, wherein the blood collection unit includes a collection container that opens in one direction,
A rubber stopper attached to the opening of the collection container so as to close the opening, and a discharge part of the blood filter is provided with a needle-like shape that penetrates the rubber stopper of the blood collection part and enters the collection container. A blood sampler having a tube. 9. The blood collection device according to claim 8, wherein the needle-like tube portion is formed integrally with the filter holding member.