JP2003202347A - Microreactor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a microreactor by which an amount of sampling blood or the like from a subject is suppressed to a minimum and by which a possibility that an operator contacts the blood or the like is reduced as far as possible. <P>SOLUTION: The microreactor is provided with a reactor mainframe 4 which comprises a channel used to pass a sample, a sample dissolving part 5 which is installed in the reactor mainframe 4 and which is used to dissolve the sample, a needle 7 which is attached to a sample injection part of the part 5 so as to be freely detachable, a filter function part 9 which is installed in the reactor mainframe 4 so as to communicate with the part 5 and which filters and removes an insoluble residue from the sample, a DNA extraction part 11 which is installed in the reactor mainframe 4 so as to communicate with the part 5, and a check valve 10 which is installed in the reactor mainframe 4 between the part 5 and the part 11. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microreactor for collecting blood or other body fluid (hereinafter referred to as blood) from a living body.

[0002]

2. Description of the Related Art Conventionally, blood and other body fluids are collected from a living body,
For detection, an operator collects blood or the like of a person under test using an injection needle, and then sends blood or the like in the injection needle to a detection jig to detect DNA or the like.

However, in this case, since the worker uses the injection needle to collect blood from the subject, there is a risk that extra blood or the like will be collected from the subject, and the worker collects blood from the subject. May come into contact with.

[0004]

SUMMARY OF THE INVENTION The present invention has been made in consideration of such circumstances, and a sample dissolving section for dissolving a sample and an injection detachably attached to a sample injecting section of the sample dissolving section. A needle, a filter function means for removing insoluble residues in the sample by filtration, a DNA elution section provided so as to be connected to the sample dissolution section, and a sample provided between the sample dissolution section and the DNA extraction section. By constructing the check valve integrally with the reactor body, the amount of blood or body fluid collected from the person being tested can be minimized and the possibility of workers coming into contact with blood, etc. is minimized. It is an object to provide a microreactor that can be used.

[0005]

The present invention is directed to a reactor main body having a flow path for passing a sample, a sample dissolving portion provided in the reactor main body for dissolving a sample, and the sample dissolving portion. Needle which is detachably attached to the sample injection part of the sample part, a filter function means which is provided in the reactor main body in connection with the dissolution part, for filtering out insoluble residues in the sample, and the reactor. A DNA extraction unit provided on the main body so as to be connected to the sample dissolution unit,
A microreactor, comprising: a check valve provided in the reactor body between the sample dissolving section and the DNA extracting section.

In the present invention, it is preferable that the reactor body further has a DNA detection section connected to the DNA extraction section. The DNA detector does not necessarily have to be integrated with the reactor body, but by integrating it, a compact microreactor capable of DNA extraction and detection can be obtained.

Further, in the present invention, it is preferable that an air escape tube is connected to the sample dissolving portion. Here, it is preferable that the tube has a small diameter so that air can enter but blood does not flow out. Due to the presence of the tube, when the injection needle is pierced into a living body such as a subject, blood or the like can be naturally sent from the living body to the sample dissolving section through the injection needle.

In the present invention, as the filter function means, when a filter provided in the reactor main body between the sample dissolving section and the DNA extracting section for removing insoluble residue by filtration is provided, or in the sample dissolving section, An example is the case where fine glass particles having such a filter function are arranged.

In the present invention, the reactor body is
It is preferable that the DNA extraction unit is provided with first and second connection ports for sending a DNA extraction reagent (washing liquid, pure water). The cleaning liquid is sent from the sample dissolving section to the DNA extracting section after removing blood from the residue, and then sent from the first connecting port to the DNA extracting section. In addition, pure water is sent to the DNA extraction unit from another second connection port. However, with the first connection port and the second connection port as one, the cleaning liquid,
Pure water may be supplied to each of the DNA extraction units.

In the present invention, the reactor main body is provided with a third connection port for connecting to the DNA extraction unit,
An external pump is preferably connected to this third connection port. In the third connection port here, the cleaning liquid and pure water are sent to the DNA extraction unit, and the DNA extraction unit
It is used to absorb other proteins and other water-soluble substances.

In the present invention, the reactor main body is provided with a fourth connection port for connecting to the DNA detection section,
An external pump is preferably connected to this fourth connection port. As a result, only the DNA can be sent from the DNA extraction unit to the DNA detection unit.

[0012]

BEST MODE FOR CARRYING OUT THE INVENTION A microreactor according to an embodiment of the present invention will be described below with reference to FIGS. 1 (A) and 1 (B). Here, FIG. 1 (A) is an overall view of the same microreactor, and FIG. 1 (B) is a sectional view taken along line XX of FIG. 1 (A). However, the materials of the constituent members described in the following examples,
Numerical values and the like are examples, and the scope of rights of the present invention is not limited thereby.

Reference numeral 1 in the figure indicates, for example, a glass substrate having a groove 1a for allowing a sample (blood) to flow therethrough. A film 2 made of, for example, polycarbonate is adhered to the glass substrate 1 to form a channel 3 for circulating blood together with the groove of the glass substrate 1.
Here, the shape of the flow path 3 is, for example, 50 to 1000 in width.
μm, depth 50 to 1000 μm. The glass substrate 1 and the film 2 are generically called a reactor body 4.

At the end of the reactor body 4, there is provided a sample lysing section 5 for lysing (destroying) red blood cells (or white blood cells) in blood. The sample dissolving portion 5 has a cavity formed by the groove formed on the surface side of the glass substrate 1 and the film 2, and the cavity contains a solvent such as an alkaline aqueous solution or a surfactant. An injection needle 7 is detachably provided in the sample injection section 6 of the sample dissolution section 5.
Here, the sample injection part 6 specifically refers to a flow path communicating with the groove part of the sample dissolution part 5 of the glass substrate 1. A thin air-releasing tube 8 is connected to the upper portion of the sample dissolving section 5 so that only air enters the sample dissolving section 5 and blood does not flow out.

A filter (filter function means) 9, a valve 10, a DNA extraction section 11 and a DNA detection section 12 are sequentially connected to the sample dissolving section 5 through the flow path 3. Here, the filter 9 has a function of filtering out insoluble residues in blood. The valve 10 functions as a check valve driven by a piezoelectric element so that blood sent to the DNA extraction unit 11 does not return to the sample lysis unit 5 side. Blood (DNA, protein, other water-soluble substances) from which the insoluble residue has been removed is sent to the extraction unit 11. In addition, the DNA extraction unit 1
1 is filled with a substance that selectively adsorbs and binds only DNA. Furthermore, reagents and the like are arranged in a matrix in the DNA detection unit 12.

In the DNA extraction section 11, a first DNA extraction reagent connection port (first connection port) 13 and a second DNA extraction reagent connection port (second connection port) 14 are provided via the flow path 3. Are connected to each other. Also, the DN
A third connection port 15 connected to an external pump (not connected) via the flow path 3 is connected to the A extraction unit 11. Here, after the blood is sent from the sample lysis section 5 to the DNA extraction section, the washing solution is sent to the DNA extraction section 11 from the connection port 13 and the connection port 1 is sent from the DNA extraction section 11.
The proteins and other water-soluble substances in the blood are sent to the 5 side. In addition, the DNA extraction unit 11 is connected from the connection port 14.
Pure water is sent to the D port using the fourth connection port 16.
Only the DNA remaining in the NA extraction unit 11 is sent to the DNA detection unit 12.

According to the above embodiment, the sample dissolving section 5, the injection needle 7, the filter 9, the valve 10, the DNA extracting section 11, the DNA detecting section 12 and the like are integrally provided in the reactor body 4. Therefore, a compact microreactor with excellent workability can be obtained. Further, compared to the conventional case, the amount of blood or the like collected from the living body can be minimized, so that the influence on the living body can be minimized, and the possibility that the worker will come into contact with blood or the like is extremely low. Therefore, safety can be increased. Furthermore, since a ready-made injection needle can be set and used in the sample injection part of the reactor main body instead of a special blood collecting device, the cost can be reduced.

In the above embodiment, the case where the filter function means is provided in the reactor main body between the sample dissolving portion and the DNA extracting portion has been described, but the present invention is not limited to this, and glass fine particles or the like on the side wall of the sample dissolving portion. A filter function means for filtering out the insoluble residue of may be arranged. Further, in the above embodiment, the case where the DNA detection unit and the like are integrally provided in the reactor main body with the sample dissolution unit, the filter function means, the valve, the injection needle, and the DNA extraction unit has been described, but the present invention is not limited to this, and at least the sample. The dissolving section, the filter function means, the valve, the injection needle, and the DNA extracting section may be provided integrally with the reactor main body.

[0019]

As described in detail above, according to the present invention, a sample dissolving portion for dissolving a sample, an injection needle detachably attached to the sample injecting portion of the sample dissolving portion, A filter function means for removing insoluble residues by filtration, and a DNA provided so as to be connected to the sample dissolving portion.
The elution part and the check valve provided between the sample dissolution part and the DNA extraction part are integrally provided in the reactor body to minimize the amount of blood or body fluid collected from the test subject. It is possible to provide a microreactor that suppresses the possibility of an operator coming into contact with blood, etc.

[Brief description of drawings]

FIG. 1 is an explanatory diagram of a microreactor according to an embodiment of the present invention.

[Explanation of symbols]

1 ... Glass substrate, 2 ... film, 3 ... flow path, 4 ... Reactor body, 5: sample dissolution section, 6 ... Sample injection part, 7 ... injection needle, 8 ... tube, 9 ... filter, 10 ... Valve, 11 ... DNA extraction unit, 12 ... DNA detector, 13, 14, 15, 16 ... Connection ports.

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Satoshi Tahara             1-8 Koura, Kanazawa-ku, Yokohama-shi, Kanagawa               Mitsubishi Heavy Industries, Ltd. Basic Technology Research Center F term (reference) 2G045 BB39 CA25 CA26 DA12 DA13                 4B029 AA07 AA27 BB20 CC03 FA12

Claims (7)

[Claims] 1. A reactor main body having a flow path for allowing a sample to pass therethrough, a sample dissolving section provided in the reactor main body for dissolving a sample, and a sample injecting section of the sample dissolving section, which is detachably attachable. An injection needle attached to, and a filter function means provided in the reactor main body in connection with the dissolution portion, for filtering out insoluble residues in the sample,
The reactor main body is provided with a DNA extraction part provided so as to be connected to the sample dissolution part, and a check valve provided in the reactor main body between the sample dissolution part and the DNA extraction part. Microreactor. 2. The microreactor according to claim 1, further comprising a DNA detection unit connected to the DNA extraction unit in the reactor body. 3. The microreactor according to claim 1, wherein a tube for air escape is connected to the sample dissolving section. 4. The microfilter according to claim 1, wherein the filter function means is a filter provided in a reactor main body between the sample dissolving section and the DNA extracting section. 5. The microreactor according to claim 1, wherein the reactor body is provided with a connection port for sending a DNA extraction reagent to the DNA extraction section. 6. The microreactor according to claim 1, wherein the reactor main body is provided with a connection port for connecting to the DNA extraction unit, and an external pump is connected to the connection port. 7. The microreactor according to claim 1, wherein the reactor main body is provided with a connection port for connecting to the DNA detection unit, and an external pump is connected to the connection port.