JP2002247917A - Artificial cultivation method for lyophyllum decastes and mixed medium for artificial cultivation of lyophyllum decastes - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a practical cultivation technology under facilities for Lyophyllum decastes, using corn flour-containing mixed medium containing totally no wheat or synthetic medium. SOLUTION: The objective artificial cultivation method for Lyophyllum decastes comprises preparing mixed medium containing at least corn flour and sawdust of broadleaf trees, inoculating the hypha of Lyophyllum decastes to the mixed medium in a moist condition and culturing the resultant product at <=30 deg.C; wherein the content of the corn flour in the mixed medium is >=10 volume % and the water content of the mixed medium put in the moist condition is 50-80 wt.%. This method enables Lyophyllum decastes to be produced at low cost.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for artificially cultivating hon-shimeji mushrooms and a hon-shimeji mushroom using a medium material that does not contain (uses) wheat or a synthetic medium (a mixture of several chemical substances). For a mixed medium.
Hereinafter, cultivation means artificial cultivation unless otherwise specified.

[0002]

2. Description of the Related Art Hon-shimeji mushrooms are mycorrhizal mushrooms that are inherently difficult to artificially cultivate. In recent years, cultivation methods using a solid medium containing wheat or a solid medium containing a synthetic medium have been developed. . For example, see Japanese Patent Publication No. 8-4427.

[0003] However, cultivation using wheat or a synthetic medium is more practical than ordinary mushroom cultivation, such as enokitake, oyster mushroom, and beech shimeji, in that the material costs are higher and the adjustment of the medium is somewhat complicated. There were difficulties.

[0004]

Under these circumstances, as in ordinary mushroom cultivation, a practical technique for cultivating using a medium material that can be obtained in large quantities at low cost and can be easily adjusted in medium is used. Development is expected.

The inventor of the present invention has proposed that hon-shimeji mushrooms form fruiting bodies by using a mixed culture medium containing no cereal flour or synthetic medium at all, and using corn flour (corn meal) and hardwood sawdust as main components. And found that the present invention was completed. Here, corn flour (corn meal) is a medium material that is used in livestock feed, is inexpensive, can be obtained in large quantities, and can easily adjust the medium such as water content.

[0006] The present invention has been made in view of such circumstances, and an object of the present invention is to provide a method for artificially cultivating hon-shimeji mushrooms using a medium material that does not contain (use) wheat or synthetic medium at all. To provide.

[0007]

Means for Solving the Problems In order to solve the problems, the present invention is a new artificial cultivation method for hon-shimeji mushrooms, comprising preparing a mixed medium containing at least corn flour and hardwood sawdust, and preparing the mixed medium. It is characterized in that fruiting bodies are generated by inoculating mycelia of hon-shimeji mushrooms in a water-moist state and culturing them at a temperature of 30 ° C or less.

[0008] A mixed medium used for artificial cultivation of hon-shimeji mushrooms, which contains at least corn flour and hardwood sawdust and is used in a wet state.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION In the embodiment of the present invention, in the cultivation method and the mixed medium having the above-mentioned constitution, it is preferable to prepare the corn flour so as to have a content of 10% by volume or more.

[0010] Here, it is practical to use corn flour commercially available for livestock feed (commonly called corn meal).

The sawdust of the hardwood may be any one of one kind of hardwood such as beech sawdust, or a mixture of several kinds of hardwood, and may be the one used in ordinary mushroom cultivation. Good.

[0012] The mixed medium contains nutrients, rice bran, bran, and nutrients used in known culture media for artificial cultivation of mushrooms.
Beer scum and the like may be added.

It is preferable to adjust the water content of the mixed medium in a water-moist state to 50 to 80% by weight, and sterilize it by heating at normal pressure or high pressure as in a normal culture medium for mushrooms.

Cultivation of the mycelium of the hon-shimeji mushroom is carried out in the same manner as in the usual mushroom cultivation. It is preferable to use a method in which the second stage of culture is carried out while maintaining the temperature at least 3 ° C. lower than the temperature.

[0015]

An embodiment of the present invention will be described in detail. The following examples are based on experimental facts, but the contents (facts) of the invention are not affected by the scale of the implementation.

Example 1 Corn flour and beech sawdust were mixed at an air-dried volume ratio of 1: 2, water was added to adjust the water content to 65 to 70% by weight, and the medium (M1 )
Was prepared (prepared).

For comparison, a medium (M2) in which barley and beech sawdust were mixed at a volume ratio of 2: 3 in an air-dried state, and water was added to adjust the water content to 65 to 70% by weight. Created.

850 ml of these mediums (M1, M2)
600 g of cultivation bottles were filled in each of the cultivation bottles, sterilized by high pressure, and two kinds of strains (Ls-H008, YG6L) of hon-shimeji mushrooms, whose fruiting body-forming ability was recognized in a barley-based medium, were preliminarily prepared using red soil and corn. The mycelium of each of the cells that had been expanded in a medium mixed with the powder was inoculated.

Next, these were cultured in a culture chamber at 23 ° C. for 60 days, the upper surface of the medium was covered with sterilized Kanuma soil, and cultured for another 7 days, and then transferred to a generation chamber at 15 ° C. and 95% humidity. Prompted the occurrence of fruiting bodies. Table 1 shows the results. In addition, the ecology of organisms related to the occurrence of these fruiting bodies is shown by photographs as substitutes for drawings. FIG. 1 shows the strain Ls-H008, and FIG. 2 shows the strain YG6L.

[0020]

[Table 1]

As shown in Table 1, FIG. 1 and FIG. 2, in the medium (M1) in which corn flour and beech sawdust were mixed, fruiting bodies were generated in all bottles free of various bacterial contamination.
This medium (M1) produced less fruiting bodies than the medium (M2) in which barley and beech sawdust were mixed, but the amount produced per bottle was 50 g or more on average and both were practical. Cultivation is possible. Specifically, strain Ls-H008
Was 60 g per bottle on average, and 55 g per bottle on average for strain YG6L. The number of days required for cultivation did not differ between the two media. The medium material cost of M1 is 1/5 or less of M2.

Example 2 A cultivation experiment was carried out under the same conditions as in Example 1 except that sawdust of deciduous broadleaf mixed with kelp and several other tree species was used instead of sawdust of beech.
Table 2 shows the results. In addition, the drawing substitute photograph which shows the ecology of the organism regarding the occurrence state of these fruiting bodies was omitted.

[0023]

[Table 2]

As shown in Table 2, in a medium (M3) containing a mixture of corn flour and sorghum and other hardwoods mixed with hardwood sawdust, fruiting bodies were generated in all bottles free of various bacterial contamination. This medium (M3) produced a smaller amount of fruiting bodies than the medium (M4) containing a mixture of hardwood sawdust containing barley, serrata and several other tree species, but produced an average of 50 g or more per bottle. Met. The number of days required for cultivation did not differ between both culture media. The number of days required for cultivation did not differ between both culture media.

Furthermore, the results in Example 2 were almost the same as the results in Example 1 performed using beech sawdust.

[0026]

The present invention has the above-mentioned constitution, and according to this, artificial cultivation of hon-shimeji mushrooms is possible on a medium in which corn flour and hardwood sawdust are mixed. Here, corn flour and hardwood sawdust have the advantage that they are inexpensive and can be obtained in large quantities, and that the preparation of the culture medium is easy. Therefore, the method of the present invention establishes and provides a practical facility cultivation technique for artificial cultivation of hon-shimeji, and is extremely valuable in industrial applicability.

[Brief description of the drawings]

FIG. 1 is a drawing-substituting photograph showing the ecology of an organism in relation to the occurrence of fruiting bodies of strain Ls-H008. M1 is a corn flour-containing mixed medium (right) used in the method of the present invention, and M2 is a conventional barley-containing mixed medium (left).

FIG. 2 is a photograph substituted for a drawing showing the ecology of an organism with respect to the occurrence of fruiting bodies of strain YG6L. M1 is a corn flour-containing mixed medium (right) used in the method of the present invention, and M2 is a conventional barley-containing mixed medium (left).

[Description of Signs] M1 Mixed Media Containing Flour M2 M2 Mixed Media Containing Barley

Claims (3)

[Claims] 1. A mixed medium containing at least corn flour and hardwood sawdust is prepared, and the mixed medium is inoculated with hon-shimeji mushrooms in a water-moist state, and cultured at a temperature of 30 ° C. or less, whereby fruiting bodies are obtained. An artificial cultivation method of hon-shimeji mushrooms, characterized in that it is generated. 2. The artificial cultivation of hon-shimeji mushrooms according to claim 1, wherein the content of the corn flour in the mixed medium is 10% by volume or more, and the water content of the mixed medium in a wet state is 50 to 80% by weight. Method. 3. A mixed culture medium for artificial cultivation of hon-shimeji mushrooms, which is capable of generating fruiting bodies by inoculating and cultivating mycelia of hon-shimeji mushrooms in a water-moist state. The corn flour content is 10% by volume or more, and the water content of the mixed medium in a wet state is 50%.
A mixed culture medium for artificial cultivation of Honshimeji mushrooms, which is about 80% by weight.