JP2002241298A - Composition effective for reducing blood glucose - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a composition having a blood glucose reducing effect. SOLUTION: This composition having blood glucose reducing effect contains Seisenryu (Cyclocarya palivrus (Batal) Ilzin skaya) as the principal ingredient.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition having a hypoglycemic effect which can be used as a medicinal or health food.

[0002]

2. Description of the Related Art Diabetes, hypertension, hyperlipidemia, gout, and the like, which are called lifestyle-related diseases, are related to long-term lifestyle habits (dietary habits, lack of exercise, overwork, stress) in addition to physical constitution. Above all, there are an estimated 6.9 million diabetic patients who are now referred to as a national illness (approximately 1.8 million of these are going to hospitals and clinics). The number reached 13.7 million (from the Ministry of Health and Welfare, Diabetes Survey).

[0003] Non-insulin-dependent diabetes mellitus (type II), which was once referred to as adult diabetes, is more likely to affect middle-aged and older people after the age of 40, and appears to affect one in four to five people. Accounts for 95% of diabetics.

Diabetes is an endocrine disease in which the function of the islets of Langerhans is impaired and glucose metabolism is disrupted due to insufficient secretion of insulin. In diabetes, if blood sugar levels are not well controlled and blood sugar is high or unstable for a long time, blood vessels and cells throughout the body will be damaged, resulting in diabetic retinopathy, diabetic nephropathy, neuropathy, etc. The disease occurs. This is where the true fear of diabetes comes from.

[0005] In this diabetes, if the blood sugar level can be controlled to a good state and the onset of complications can be prevented, the health can be maintained, there is no inconvenience in daily life, and the illness is not felt. It is.

[0006] In Chinese medicine (Chinese medicine), diabetes is called extortion, and as a means of improving this exudation, white VS silkworm, which is a crude drug (animal) of the silkworm family, has been conventionally known in China. ing.

[0007]

SUMMARY OF THE INVENTION An object of the present invention is to provide a composition having a higher blood glucose lowering action than that of the white VS silkworm.

[0008]

Means for Solving the Problems The present inventors sought a component having a higher blood glucose lowering action than that of the white VS silkworm, and as a result of repeated studies, they came to the knowledge that Seienyanagi is more effective.

[0009] That is, the present invention is a composition having an effect of lowering blood glucose, which is characterized by containing a green liquor as a main component.

[0010] The present invention is characterized in that the present invention comprises a blue-green willow as a main component, and further contains one or more components selected from the group consisting of white VS silkworm, mountain medicine, ground yellow leaf, yellow sperm and mulberry leaf. It is a composition having a hypoglycemic effect.

Further, the present invention is the above composition, further comprising one or more components selected from the group consisting of Kita-oki, Kakkon, zinc yeast and chromium yeast.

[0012] The present invention also relates to a blue-green willow (27.95 wt%), a white VS silkworm (11.03 wt%), a herbal medicine (8.82 wt%), and a ground yellow stem leaf (8.82 wt%).
%, Yellow spirit: 5.88wt%, mulberry leaf: 5.88wt%, Kitokigi leaf: 5.88wt
%, Radish: 5.15 wt%, zinc yeast: 14.71 wt%, and chromium yeast: 5.88 wt%.

[0013] Dehydration in Chinese physicians is mainly caused by
It is thought that it is due to "insufficiency of tsu liquid". In other words, it is a disease in which the heat inside the body rises and causes a shortage of water. The principle of treatment is "Shinin Ikutsu, Seishin Yuki", and the main body is to cool down the body heat, replenish water, and replenish energy. For this reason, treatment is performed with a focus on correcting abnormalities in the whole body, including glucose metabolism.

On the other hand, Western physicians use FBS (Fasting Blood Glucose) as a pathological index for diabetes.

[0015]

[Outside 1] (Hereinafter, substitute 1 for HbA1c), insulin, 1.5AG
In addition, drug treatment, treatment of stimulating residual β cells to promote insulin secretion, suppressing insulin hyperglycemia secretion, and affecting the process of glucose metabolism are being performed. Their purpose is to lower or control blood sugar, but not to fundamentally solve the lack of function of the islets of Langerhans.

Therefore, a fasting blood glucose and glucose tolerance test, which is a basic pharmacological test of a liquorice syrup composed mainly of Qianyan willow, was carried out, and the results are shown in Tables 1, 2 and 1 and 2.

The effect of alloxan diabetic rats on fasting blood glucose is shown in Table 1 and FIG. 1, as shown in Table 1 and FIG. The difference was clearly stronger than the drug (Kaikyo Maru).

The results of the glucose tolerance test on alloxan diabetic rats are shown in Table 2 and FIG. Yanagi Suicen clearly enhances glucose tolerance in alloxan diabetic rats, and the blood glucose level of the drug administration group is clearly lower than that of the model control group. The blood sugar lowering effect of Yanagi Sugar is stronger than that of Shokyo Maru.

(Explanation of Terms) HbA1c: Also called glycated hemoglobin. It is a non-enzymatic combination of hemoglobin in red blood cells and glucose in the blood, which makes up the majority of adult hemoglobin and is most sensitive to changes in blood sugar. Mean blood glucose from two months before blood collection.

1.5AG (anhydroglucitol): One of the polyols very similar to glucose. When glucose is increased in blood, reabsorption is hindered similarly to sugar, and the amount excreted in urine increases. With this index, the average of the blood sugar level from three to four days ago can be found.

Alloxan Diabetes: This is considered to be experimental diabetes and is most commonly produced in rats. Alloxan occurs as a result of selectively destroying beta cells of the islets of Langerhans of the pancreas and stopping insulin production.

[0022]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention is based on the above findings, and is characterized by mainly comprising a green liquor. Yanagi Su-Sen crushes peas, willow, radix, etc.
The mixture was mixed, boiled with distilled water, and concentrated to obtain an aqueous solution. As described above, the improvement of the tissue of the islets of Langerhans in diabetic rats was observed by taking the willow sugar sacrifice containing the main component of blue-green willow as described above, and particularly in the fasting blood glucose and glucose tolerance tests, the hypoglycemia was remarkable. The results of animal experiments are shown below. <Experiment on hypoglycemia and glucose tolerance (sugar tolerance test)> (Method) Samples (i) Koushou Maru (Guangzhou Chuyaku-Thats Lot No. 94020181Q18) (ii) Alloxan (Sigma Corp. Lot No. 110H3367) (iii) Glucose oxidase assay sample (Nanchang Baixin New Technology Business Corp. Lot No. 940402) 2 . Preparation of Diabetic Model and Grouping of Large Rats Wistar male rats (180 ± 15 g, provided by Jiangxi Medical Experimental Animal Center) were administered ip 60 mg / kg of alloxan for 72 hours.
Thereafter, blood was collected from the tail vein, and fasting blood glucose was measured. Blood glucose level
Rats with 13.88 mmol / 1 or more were selected and used in the experiment. Thirty matched diabetic rats were divided into three groups, and 10 rats in each group (the difference in the average blood glucose level of the groups was 0.56 mmol / 1 or less) were used as the diabetic model control group, the Shokanmaru group (1 g / kg), The group was a sugarcane group (1 g / kg). Normal Wistar male rats having the same body weight were taken and used as a physiological saline control group. Once daily for all rats
ml / 100g drug, willow sugar, saline for 4 consecutive weeks
Administration. 3. Laboratory index Fasting blood glucose of the rats was measured once a week. Measurement value is 12hr
Were fasted, blood was collected from the tail vein, and serum glucose was measured by the oxygen method. After the final administration of the drug, a glucose tolerance test was performed. That is, fasting blood glucose was measured first, and the blood glucose level before administration was determined. Then, 2 g / kg of glucose was administered ip, and the drug was given separately. After 0.5, 1, and 2 hours, blood was collected from the tail vein and the blood sugar level was measured. Finally, the mice were sacrificed and dissected immediately, and the pancreas was excised and fixed with 25 ml / l formalin. The pancreas was used as a paraffin section, subjected to HE staining, and observed under a microscope for changes in the islets of Langerhans.

In the histopathological examination, when a section of the pancreas specimen was examined under a microscope, the boundaries of the islets of Langerhans in the model control rats were unclear, the cell arrangement was irregular and blurred, and the staining of some cell nuclei was unclear. , Deeply stained, the nuclei were firmly concentrated. However, the tissue of the Langerhans islets of the rats in the group administered with willow sugar was similar to the tissue of the Langerhans islets of normal rats, with well-defined boundaries, clear nuclei, well-arranged cells, and abundant clots. Was. This indicates that the tissue on the islets of Langerhans of alloxan diabetic rats to which willow sugar was administered gradually recovered and improved. <Clinical follow-up observation test> As a result of conducting a controlled study at six hospitals: Jiangxi Medical School First Hospital, Jiangxi Province Middle School of Medicine, Nanchang City Second Hospital, Shanghai City First People Hospital, Huaxi Medical University First Hospital, and Beijing Xiyuan Hospital, The efficacy and safety were confirmed in 283 non-insulin-dependent diabetes patients.

As a result, the fasting blood glucose after the treatment was 2 days after the meal.
There was a clear drop in urinary sugar and improvement in symptoms over time and 24 hours, and cholesterol and glycenol were down. And no side effects were seen.

2. A clinical trial of Yanagi Tousen was performed at Sanno Hospital General Internal Medicine (Akasaka, Tokyo). The progress and observation results are as follows.

The changes in indices deeply related to blood glucose in the five subjects are shown in the graph. (FIGS. 3 to 6) The fasting blood glucose level gradually decreased except in one case (KC), and showed a decrease of 24 to 99 mg / dl by 24 weeks after the start of administration, respectively. . HbAlc showed the same tendency, and decreased in all four cases over time. One case (KC) in which neither blood glucose nor HbAlc decreased showed no change in blood glucose,
c also temporarily decreased but returned to normal after 16 weeks. In contrast, 1.5AG continued to increase after 4 weeks. However, there was an example (HO) that decreased after 4 weeks. Insulin also continued to increase in all cases.

Other urinary and blood test indices are GP
Decreases in T, γ-GTP, etc. were scattered, but no increase in liver function test values was observed in any of the cases.

Based on the above results, the results are summarized in a table (Table 3).

All of the five patients who received Licorice were effective, and three of them showed remarkable effects. One case in which neither blood glucose nor HbAlc decreased (KC)
Was a patient who was unable to diet at all. No side effects were observed in any of the cases.

[0030]

[Table 1]

[0031]

[Table 2]

[0032]

[Table 3]

[0033]

According to the present invention, a composition having a high blood glucose lowering effect was obtained.

[Brief description of the drawings]

FIG. 1 is a graph showing the results of a test on fasting blood sugar of a willow sugar sacrifice containing a green plum willow as a main component.

FIG. 2 is a graph showing the test results for the sugar tolerance capacity (sugar tolerance test) of Yanagi Sugar Cane mainly composed of Qianyan willow.

FIG. 3 is a graph showing the changes in fasting blood glucose observed after administration of 5 willow subjects at the Sanno Hospital General Internal Medicine (Akasaka, Tokyo), to which willow sugar sens mainly composed of blue-green willow.

Fig. 4. Yanagi sugar-sen, mainly composed of blue-green willow, is administered to five subjects at Sanno Hospital General Internal Medicine (Akasaka, Tokyo).
The graph which observed HbA1c and showed the change.

FIG. 5: Five subjects were given a willow sugar sensin mainly composed of blue-green willow at Sanno Hospital General Internal Medicine (Akasaka, Tokyo).
The graph which observed 1.5AG and showed the change.

FIG. 6 is a graph showing changes in insulin administered to five subjects at Sanno Hospital General Internal Medicine (Akasaka, Tokyo).

Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat II (reference) A23L 1/30 A23L 1/30 Z A61K35 / 64 A61K 35/64 35/72 35/72 A61P 3/10 A61P3 / 10 (72) Inventor Shinya Aoki 2-7-18 Nakase, Kawasaki-ku, Kawasaki-shi, Kanagawa F-term (reference) 4B018 MD48 MD61 MD76 MD81 ME03 4C087 AA01 AA02 BB21 BC11 MA02 MA52 NA05 ZC35 ZC75 4C088 AB12 AB37 AB59 AB84 AB85 AC01 MA07 MA52 ZC35 ZC75

Claims (4)

[Claims] 1. A composition having an effect of lowering blood sugar, characterized by comprising a green liquor as a main component. 2. A composition mainly composed of a green senyan willow, and further selected from the group consisting of white VS silkworm, mountain medicine, ground yellow leaf, yellow sperm and mulberry leaf.
A composition having the effect of reducing blood glucose, comprising the above components. 3. The method according to claim 1, further comprising one or more components selected from the group consisting of Kitokihagi, Kakkon, zinc yeast and chromium yeast. Composition. [4] Seisenyanagi: 27.95wt%, White VS silkworm: 11.03wt
%, Mountain medicine: 8.82wt%, ground yellow stem leaf: 8.82wt%, yellow spirit: 5.88
wt%, mulberry leaf: 5.88wt%, Kitokigi leaf: 5.88wt%, kakkon: 5.15
wt%, zinc yeast: 14.71 wt% and chromium yeast: 5.88 wt%
A composition having an effect of lowering blood glucose, comprising: