JP2002223726A - Food for beauty - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a food for beauty remarkably improving a skin beautifying function of hyaluronic acid. SOLUTION: This food for beauty comprises hyaluronic acid, glucosamine, oligosaccharide and vitamin C as active ingredients. Ingestion of the food for beauty is useful for the skin to retain water and maintain its smoothness, and effective in beautifying the skin.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a beauty food, and more particularly to a beauty food containing hyaluronic acid.

[0002]

BACKGROUND OF THE INVENTION Prevention of rough skin and improvement of fine wrinkles are desired by all human beings, and various cosmetics for skin and the like have been developed.

[0003] These cosmetics are basically formed in consideration of the composition and composition of the skin. When the composition and composition of the skin are specifically described, the skin is composed of epidermis, dermis, and subcutaneous cell tissues. In the epidermis, there are melanocytes, Langer's cells, etc. in addition to the epidermal cells, and the outermost surface of the epidermis has a very strong and stable cytoskeletal protein called keratin in the stratum corneum and keratin intercellular lipids. Contains an amino acid such as sodium pyridonecarboxylate, which is called a natural moisturizing factor, and plays an important role in the moisturizing function of the skin. For this reason, these natural moisturizing factor-like substances have been used in cosmetics and cosmetics as moisturizing materials.

[0004] The dermis of the skin is composed of fibrous, matrix and cellular components. The fibrous component is composed of collagen fibers, elastic fibers, reticulum fibers and the like, and the dermal matrix is an amorphous component filling the space between the fibrous components. There, the cell component consists of fibroblasts that produce fibrous components and matrix, among which collagen collagen collagen is an important component occupying 75% of the dry weight of the dermis, and is related to the elasticity of the skin, It decreases with age and causes the skin to lose its elasticity, causing skin lumps and wrinkles. For this reason, cosmetics for beautiful skin which are contained in cosmetics to replenish collagen have been developed. Collagen has also come to be taken orally as a health food, but its effect as a beauty food has not been confirmed.

Further, there are mucopolysaccharides such as hyaluronic acid and dermatan sulfate as functionally important substances in the skin matrix, and hyaluronic acid is rich in water retention compared to other mucopolysaccharides. It has a role to maintain the elasticity and elasticity, and it decreases with age like collagen, so cosmetics for beautiful skin have been developed to contain hyaluronic acid, which is an important factor for beautiful skin, in cosmetics. . Among them, Japanese Patent Application Laid-Open Nos.
As described in Japanese Patent Application Laid-Open No. 000-102362, some foods containing hyaluronic acid as a composition are orally ingested. In the foods described in the above-mentioned Japanese Patent Application, high-molecular-weight hyaluronic acid is subjected to high-temperature and high-pressure treatment so as to facilitate intestinal absorption. Or, when it is reduced in molecular weight by enzymatic treatment and taken orally, it exerts a beautiful skin effect by various activating effects.

[0006]

However, in the above cited document, there is almost no confirmed data that hyaluronic acid is used in the body of an animal, and furthermore, it is required to further enhance the action of beautiful skin.

[0007] The present invention is a cosmetic food made for the purpose of significantly improving the beautiful skin effect of hyaluronic acid.

[0008]

The cosmetic food of claim 1 of the present invention contains hyaluronic acid, glucosamine, oligosaccharide and vitamin C.

[0009] The beauty food according to claim 2 of the present invention is characterized in that
Weight ratio of hyaluronic acid to 0.01 to
0.25, the weight ratio of glucosamine is 0.005 to 0.1
5. The weight ratio of oligosaccharide is 0.02 to 0.35, and the weight ratio of vitamin C is 0.01 to 0.25.

[0010] The cosmetic food according to claim 3 of the present invention has a molecular weight of hyaluronic acid of 600,000 to 2,000,000.

[0011] The cosmetic food according to claim 4 of the present invention is one in which hyaluronic acid is extracted from a chicken cop.

[0012] In the beauty food according to the fifth aspect of the present invention, the hyaluronic acid may contain Streptococcus zoopidemicus, Streptococcus.
It is produced by fermentation of at least one microorganism of equisimilis.

[0013] The beauty food according to claim 6 of the present invention is characterized in that glucosamine is produced by hydrolyzing chitin.
-Deoxy-D-glucose or 2-amino-2-deoxy-
It consists of at least one of the salts of D-glucose.

[0014] The cosmetic food according to claim 7 of the present invention is characterized in that the oligosaccharide comprises at least one of maltooligosaccharide, galactooligosaccharide, fructooligosaccharide, isomaltooligosaccharide, gentiooligosaccharide, lactosucrose and trehalose.

When the cosmetic food of the present invention is taken orally,
Due to the synergistic effect of the components of hyaluronic acid, glucosamine, oligosaccharides and vitamin C, excellent cosmetic effects such as enhancing production of hyaluronic acid and collagen in the skin can be produced.

This is thought to be due to the following actions of glucosamine, oligosaccharides and vitamin C.

That is, it has been shown that glucosamine can be converted into proteoglycan such as hyaluronic acid in the body. Therefore, ingestion of glucosamine has a small effect on intestinal bacteria directly like hyaluronic acid, but hyaluronic acid in the body. It is considered to be converted into an acid and to increase hyaluronic acid in the skin, in particular, to exert a cosmetic effect. In addition, since hyaluronic acid has a high viscosity due to its high molecular weight and cannot be ingested in large quantities at a time, it is thought that ingestion of a large amount of glucosamine assists its effect, and if it is ingested simultaneously with hyaluronic acid, it is considered that the action of hyaluronic acid is increased.

Vitamin C has an antioxidant effect, an active oxygen scavenging effect, an effect of inactivating viruses, and the like. For beautiful skin, it is greatly involved in the production and maintenance of collagen, and is essential for the proliferation of skin cells. In addition, vitamin C has such an antioxidant effect because it suppresses the process of producing melanin from tyrosine, which is the cause of spots, and has the effect of slowing down skin aging and maintaining fresh skin. Is effective in stabilizing hyaluronic acid, and it is considered that the effect is further enhanced when taken simultaneously with hyaluronic acid and glucosamine.

Oligosaccharides reach the large intestine without being decomposed when taken orally, and are used by intestinal bacteria to change the bacteria constituting the intestinal flora so that useful lactobacilli and bifidobacteria are increased. In addition, it produces short-chain fatty acids such as lactic acid and acetic acid to acidify the pH in the large intestine, thereby preventing constipation and exerting a beautiful skin effect by regulating the body well. In addition to hyaluronic acid, glucosamine and vitamin C, Concomitant use of oligosaccharides further improves intestinal flora and further enhances the action of hyaluronic acid.

In addition, hyaluronic acid and oligosaccharides are not digested by digestive enzymes and reach the large intestine as they are, where they are used by intestinal bacteria such as lactobacilli and bifidobacteria,
It promotes the growth of these bacteria and produces fatty acids such as acetic acid and lactic acid. It is considered that these fatty acids acidify the pH in the intestine to prevent constipation and prevent colon cancer and the like. In addition, these useful Lactobacillus and Bifidobacterium also have a function of inhibiting the growth of harmful bacteria such as spoilage bacteria and purifying the intestinal environment. In addition, these correspond to prebiotakes.
“Prebiotics, an indigestible food ingredient that promotes the growth of useful bacteria in the colon or suppresses the growth of harmful bacteria, resulting in a beneficial effect on the health of the host through intestinal purification” (Mitsuoka Shirashi; intestinal flora and health p171,
Society Center).

As described above, the hyaluronic acid and collagen of the skin are increased, the moisturizing effect and elasticity are increased, the useful bacteria of the intestinal flora are increased, and the body is brought into a healthy state. It seems to have led to improvement. Until now, cosmetics have been applied to the skin to improve the skin. However, adverse effects may occur in the case of sensitive skin due to the effects of surfactants, preservatives and the like. In contrast, the safety of each component of the product of the present invention was confirmed as food.

Further, when the molecular weight of hyaluronic acid is from 600,000 to 2,000,000, the beauty food of the present invention increases the number of lactobacilli and bifidobacteria in the intestine as compared with those having a low molecular weight of 500,000 or less. Bowel action can be enhanced and cosmetic action can be further enhanced.

When hyaluronic acid is extracted from a cockscomb or produced by fermentation of microorganisms, high-pressure, high-temperature, and enzyme treatments conventionally required to reduce the molecular weight of hyaluronic acid to 500,000 or less are unnecessary, and the production cost is reduced. Can be reduced.

Glucosamine is converted to 2-amino-2-deoxy-
D-glucose or 2-amino-2-deoxy-D-glucose salt, wherein the oligosaccharide is at least one of maltooligosaccharide, galactooligosaccharide, fructooligosaccharide, isomaltooligosaccharide, gentiooligosaccharide, lactosucrose and trehalose With the above configuration, the cosmetic effect can be enhanced and the manufacturing cost can be further reduced.

[0025]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, an embodiment of a beauty food of the present invention will be described.

When producing the cosmetic food of the present invention, first, hyaluronic acid, glucosamine, oligosaccharide, vitamin C (ascorbic acid), excipients and the like are prepared.

Here, the prepared hyaluronic acid is obtained by chopping the cockscomb in a raw state, treating it with enzymes such as lipase and protease, and decomposing fat contained in the cockscomb to obtain a molecular weight of 600,000 to 200,000. Manufactured in high purity at 10,000, Streptococcus zoopidemicus, Streptococcus
It may be produced by fermentation of a microorganism such as equisimilis. It should be noted that hyaluronic acid naturally has high safety and has passed the standards for food additives.

The prepared glucosamine mainly hydrolyzes chitin contained in shells of crustaceans with concentrated sulfuric acid to produce monosaccharide N-acetylglucosamine and removes the acetyl group of N-acetylglucosamine. Thus, it is produced as a hexosamine such as 2-amino-2-deoxy-D-glucose or a hexosamine salt such as a hydrochloride or a sulfate of 2-amino-2-deoxy-D-glucose. Hydrochloride to crystallize glucosamine,
Alternatively, those manufactured in the form of sulfate are used. In addition, glucosamine is a component such as proteoglucan widely distributed in cartilage and connective tissue in the animal body, and there are cases where it is ingested orally and used as a treatment for acute and chronic arthritis, which is highly safe. Things.

The oligosaccharide to be prepared is not particularly limited, but may be selected from maltooligosaccharides, galactooligosaccharides, fructooligosaccharides, isomaltooligosaccharides, gentiooligosaccharides, lactosucrose, trehalose and the like. We use those that are manufactured and distributed.

Further, as the excipient, those selected from dextrin, corn starch, lactose, starch syrup such as reduced malt, sucrose ester, palatinose, gelatin, cellulose, etc., erythritol, glucose, xylitol, mannitol and the like are used. , As appropriate, citric acid, tartaric acid, etc.
pH adjusters, various flavors, preservatives such as sorbic acid, emulsifiers and the like are used.

Hyaluronic acid, glucosamine, oligosaccharides,
After preparing vitamin C, excipients, etc., they are weighed to a predetermined amount and mixed by a stirrer, and the mixture is compressed into a predetermined shape by tableting using a tableting machine. After drying and sorting, the product is coated, coated, air-dried, etc. to complete the cosmetic food tablets. The cosmetic food can be not only tablets but also solid preparations such as granules, fine granules, powders, capsules, and troches, and liquid preparations such as emulsions, syrups, and suspensions.

Here, the weight ratio of each component of hyaluronic acid, glucosamine, oligosaccharide, vitamin C, etc. contained in the beauty food is such that hyaluronic acid is 0.1% to 1 weight of the whole.
Glucosamine is 0.001 to 0.25, preferably 0.01 to 0.25, preferably 0.05 to 0.20.
15, preferably 0.020 to 0.100, and 1
0.02 to 0.35, preferably 0.05 to 0.30, of oligosaccharide based on the weight of vitamin C, and 0.01 to 0.25, preferably 0.1 to 0.2 of vitamin C, based on the total weight of 1. 05-
0.25.

Next, each test using the beauty food of the present embodiment will be described, but the present invention is not limited to these tests. In addition, the compounding ratio in the test is indicated by weight% unless otherwise specified.

<< Test 1 >> Test 1 is a digestion and absorption test of hyaluronic acid using rats. 1) Test plot and test method A digestion and absorption test of hyaluronic acid (hereinafter, referred to as HA) using rats was performed as follows. Initial weight 65g (0.
64N) Wistar male rats inside and outside were used as test animals, and 10 rats were used per group. The test group used a feed having the composition shown in Table 1.

Feed composition (%)

[Table 1]

Test plot 1 was a control diet to which 2% of cellulose powder was added, and test plot 2 was a diet 2% of high-molecular-weight HA to which 2% of high molecular weight HA derived from cockscomb was added instead of cellulose powder. Test section 3 is for comparison with low-molecular-weight HA 2% with 2% addition of low-molecular-weight HA having a molecular weight of 50,000 to 100,000, test section 4 is for comparison with the present invention 2% section, and test section 5 is for comparison with the present invention section. Containing hyaluronic acid and glucosamine (hereinafter HAG)
2%. Here, the product of the present invention used was 1
60 g of hyaluronic acid (0.5 g in 9.80 N)
9N), glucosamine hydrochloride 20g (0.20N), isomaltooligosaccharide 100g (0.98N), vitamin C12
It contained 0 g (1.18 N). Each group was preliminarily reared for one week and then reared on each test meal. Food and drinking water were available ad libitum.

2) Digestion test The digestion test was performed at the age of 3 weeks after the start of breeding. Table 2 shows the results. Numerical values are shown as mean ± standard deviation. The quantification of HA in feces was performed as follows. (Extraction method from feces) 500 mg (4.90 × 10 ⁻³ N) of dry powdered feces was precisely weighed, defatted with a mixed solution of chloroform: methanol = 2: 1, and further with ethanol. This was suspended in distilled water, trichloroacetic acid was further added for deproteinization, and the mixture was cooled, centrifuged at 4 ° C., and the supernatant was dialyzed. (Electrophoresis method) The above test solution was subjected to the method of Hata and Kohata (Anal. Biochem.,
45 462 (1972)), and hyaluronic acid was analyzed by an electrophoresis method using a cellulose acetate membrane.
Hyaluronic acid was added at 0.10 mg / ml, 0.25 mg / ml and 0.2 mg, respectively.
After dissolving to a concentration of 50 mg / ml, spotting 3 μl as a standard solution and performing electrophoresis, the concentration of each standard solution is measured by densitometer to prepare a standard curve. The same electrophoresis was performed by spotting 3 μl of the test solution, and the concentration of hyaluronic acid was calculated from the standard curve.

Absorption test

[Table 2] As for the feed intake, the test plot 1 shows the intake as cellulose, and the test plots 2 to 5 show the intake as hyaluronic acid.

Analysis The daily feed intake was not much different from the diet of the present invention, the HAG diet, the hyaluronic acid diet, and the control plot. Excretion amount of the present invention is lower in the diet group than in the control group,
The hyaluronic acid group was clearly lower than the control group. This is considered to be due to the fact that hyaluronic acid was digested while the cellulose in the control group was not digested and excreted, so that the total excreted feces was reduced. The amount of hyaluronic acid in feces ranged from 1.4 to 2.5 mg / day. Calculations from this show that the ingested hyaluronic acid has been digested almost in some way. There was no difference in absorption rate due to the molecular weight of hyaluronic acid. From this result, it became clear that hyaluronic acid was digested and absorbed regardless of the molecular weight and used as food. It is presumed that the amount of water in the control group is large because the excreted cellulose has a form that embraces water.

<< Test 2 >> In Test 2, the effect of the product of the present invention on intestinal bacteria was examined for Lactobacillus bacteria and Bifidobacteria, which are typical intestinal bacteria.
The animals were bred with the feed composition of Test Group 1 to Test Group 4 in Table 1 and blood was collected from the heart under membrane anesthesia on day 45 from the start of breeding. Immediately, the cecum containing the contents was removed, and 1 g of the contents of the cecum was mixed with 9 ml of an anaerobic diluent under ice cooling. This was used as a 10 ^-1 diluent and serially diluted 10-fold to prepare 10 ^-1 to 10 ^-8 diluents. The bacterial flora in the cecum was measured according to the method of Mitsuoka. Lactobacillus and bifidobacteria were cultured using GAM bouillon (manufactured by Nissui), and bifidobacteria were cultured by the gas pouch method as a simple anaerobic culture method.

The number of bacteria is represented by a common logarithm (for example, 8 represents 10 ⁸ ) per 1 g of cecal contents. The results are shown in Table 3. Each measured value is shown as the average of the number of bacteria ± standard deviation.

Changes in intestinal bacteria

[Table 3]

In the case of Lactobacillus bacteria, the increase was clearly 10 times or more in the high-molecular-weight hyaluronic acid diet as compared with the control. Although no significant difference was observed in the low-molecular-weight hyaluronic acid diet, the present product diet was higher than any of the diets.
In addition, no increase was observed in the HAG diet, and it did not reach the diet of the present invention. On the other hand, in the case of the Bifidobacterium, the increase in the high-molecular-weight hyaluronic acid diet was clearly 70-fold greater than that in the control group, and the increase in the low-molecular-weight hyaluronic acid diet was 10-fold or more. The increase in the diet of the present invention was remarkable, and the increase was 10 or more in the low molecular weight hyaluronic acid diet. HA
The increase was also observed in the G diet, but not as high as that of the diet diet of the present invention.

<< Test 3 >> In Test 3, the amount of hyaluronic acid in the skin was measured. Extraction and quantification of hyaluronic acid in the skin was carried out by the method of Kihana et al.
Kanamaru et al. (45)
The following was conducted in accordance with the abstract of the SCCJ meeting, 45). 45 days after breeding, the skin diameter of the rat head is 1.15 cm.
Was removed, and the dermis portion from which the subcutaneous tissue was removed was finely cut. Acetone was added to the skin pieces to remove lipids, and after vigorous stirring, the skin pieces were precipitated by centrifugation (3 times). Acetone was completely removed under reduced pressure of the water flow all day and night. Distilled water was added to the mixture, and the mixture was boiled for 10 minutes to inactivate the endogenous enzyme, and then pulverized with a glass homogenizer and freeze-dried. The dried product was completely dispersed in 0.5N caustic soda,
Shake for time to solubilize. Adjusted to pH 7.5 with hydrochloric acid,
An equal volume of 50 mM Tris-HCl buffer (pH 7.5) was added. Actinase (5 mg / ml) was added to degrade proteins bound to hyaluronic acid,
Digested for hours. Add half the amount of actinase from the previous day,
Digestion was performed again at 50 ° C. for 24 hours. To remove protein, trichloroacetic acid was added to a final concentration of 10%, the mixture was allowed to stand at low temperature, and then centrifuged at 10,000 xg for 30 minutes at 4 ° C to collect a supernatant. Ice-cooled 1.3% of 3 times the supernatant
Hyaluronic acid was made insoluble by adding potassium acetate / ethanol, and centrifuged at 10,000 × g for 15 minutes to obtain hyaluronic acid. The quantification of hyaluronic acid was performed by the above-mentioned electrophoresis method. Table 4 shows the results.

The amount of hyaluronic acid in the skin (mg / g dry weight)

[Table 4]

The amount of hyaluronic acid in the skin of the diet of the present invention was increased as compared with the control. As a result, it was clarified that there was an effect on the moisture retention and elasticity of the skin.

Test 4 Test 4 is a clinical study of the product of the present invention on humans as follows. [Preparation method of food used in this test]: Ingredients in one grain Hyaluronic acid 30 mg (2.94 × 10 ⁻⁴ N) Glucosamine 10 mg (0.98 × 10 ⁻⁴ N) Isomaltooligosaccharide 50 mg (4.90) × 10 ⁻⁴ N) Vitamin C 60 mg (5.88 × 10 ⁻⁴ N) Excipient 100 mg (9.80 × 10 ⁻⁴ N) The above components are mixed and dried, and then palatinose, gelatin, etc. 5
It was sugar-coated with 00 mg (4.90 × 10 ⁻³ N). [Subject]: A woman suffering from dry skin and rough skin. Placebo group: 15 people, product group of the present invention: 1
There were three people. [Test method]: (1) Test food: the above-described present product, the present product and appearance,
Simulated foods (placebo group) having the same taste (odorless) were ingested daily for 9 weeks and 3 tablets for 6 weeks. (2) Inspection method: Dermatological examinations and interviews: pruritus,
Dryness, flushing, erosion, desquamation, papules, small blisters, and swelling, and facial findings were evaluated for makeup rash, dryness, flushing, and makeup glue on a four-point scale. In addition, comprehensive findings of facial and systemic findings were evaluated as general symptoms. 2. Water content, oil content, acidity (pH): Water content is Courag
Corneometric CM from e Khazaka Electronic Gmbh
825. Oil content is Courage Khazaka
The measurement was performed using a Subumeter SM810 manufactured by Electronic Gmbh. Acidity is Courage Khazaka Electronic Gmbh
The measurement was carried out using PH900 manufactured by the company. The measurement site is below the left eye 1
cm, left upper arm inner part (3 cm above the elbow) and cervical back (3 cm below the spinous process of the neck). In addition, in order to keep the environment as identical as possible, a room (room temperature 18-20 ° C., humidity 45-60%) set under constant conditions was prepared before the measurement, and the subjects were allowed to rest for 30 minutes or more. . Measurements were taken at the same time as possible. Inspection of makeup at the measurement site is in principle 6
Banned 0 minutes ago. 3. Analysis with a microscopic three-dimensional skin surface analyzer: Courage Khazaka Elec
skin surface digital analyzer (VISIOS manufactured by tronic Gmbh)
CAN) irradiates the surface of the skin with special ultraviolet rays, and digitally processes and evaluates the image. 1) SEsm (Skin Smoothness): One of the indicators of skin smoothness. Calculated from wrinkle width, depth and average. The lower the value, the smoother it is. 2) SEr (Skin roughness): This is one of the indexes indicating skin roughness. The ratio of points darker than the set points in the entire image was calculated and calculated. The lower the numerical value, the smoother the surface. The higher the numerical value, the coarser the surface. 3) SEsc (Skin Scaliness): One of the indicators of scale (the degree of keratin drying). The portion where the skin has peeled off is counted brightly in the image, and the proportion of the portion that is brighter than the set value in the whole is calculated. The lower the value, the more turbid and less exfoliation (scale). 4) SEw (Skin Wrinkle): One of the indexes indicating the number and width of skin wrinkles. Vertical and horizontal texture of the skin,
Alternatively, it indicates the number and width of wrinkles. The higher the value, the more wrinkles and the wider the wrinkles. 5) Kurtosis (correction K): indicates the smoothness of the entire skin. Indicates the quality of the histogram of the skin tone point, with a value of 0
The closer to, the smoother the histogram of the color tone point is, indicating an ideal skin.

[Clinical Test Results] (1) Dermatological Examination Results: Table 5 shows changes in the results of the dermatological examination before and after ingestion. Each value indicates the average value of the individual scores of the four-level evaluation from “0 (no symptom)” to “3 (severe)” for convenience. The lower the score over time, the better. With respect to the facial symptoms, significant improvement was observed 3 weeks and 6 weeks after ingestion of the observation item of "drying of facial skin" in the product group of the present invention. No significant improvement in facial symptom evaluation was observed in the placebo group. Further, in the whole body findings, significant improvement was observed in "pruritus", "dryness" and "flushing" in the product group of the present invention. On the other hand, the placebo group showed a significant improvement in “dryness”, but no significant improvement was seen in the other evaluation items.

Dermatological examination results

[Table 5] The value indicates the average value of each subject (statistical test is non-parametr
ic)

(2) Results of water content, oil content and acidity: Table 6 shows changes in water content, oil content and acidity before and after intake.
In the placebo group, a significant decrease in the water content was observed at 6 weeks after the left eye in the placebo group, whereas in the group of the present invention, in contrast, a significant increase in the water content was observed after 6 weeks. Since the oil content was 0 in most subjects except for the lower part of the left eye, the measurement of the left upper arm and the back of the neck was excluded from the second examination. As a result, a significant decrease in the oil content was observed in the product group of the present invention.

Measurement results of moisture, pH and oil content of skin

[Table 6] The value indicates the average value of each subject (statistical test is non-parametr
ic)

(3) Analysis result by microscopic three-dimensional skin surface analyzer (VISIOSCAN): Table 7 shows the transition of each parameter value image-analyzed by VISIOSCAN. In the SEsm value, the product group of the present invention shows a significant improvement in the back of the neck,
It was shown that the smoothness of the skin was restored. On the other hand, no improvement was observed in the placebo group. In the case of SEsc, significant recovery was observed in the present invention group in the back of the neck, but no significant recovery was observed in the placebo group.
In addition, Kurtosis showed a worsening tendency in the upper left region of the placebo group, while the Kurtosis group showed an improvement in the case of the present invention group, and obtained a contrasting result. There was no significant change in SEr and SEw in both groups.

Parameter values measured by VISIOSCAN

[Table 7] The value indicates the average value of each subject (statistical test is non-parametr
ic)

Test 5 Test 5 shows the condition of the skin when the product of the present invention is used in the panel list and the presence or absence of a cosmetic effect of bowel movement. After mixing the components at the mixing ratios shown in Table 8, the food composition of the present invention was granulated by a conventional method. Forty days each of the female compositions of each test group were ingested ⁴ g (3.92 × 10 ⁻⁴ N) for 60 days by female panelists for 60 days, and changes in skin condition and bowel movement were observed. The results after 60 days are shown in Table 9.

Food mix ratio (%)

[Table 8]

Panel test result (person / 40)

[Table 9]

Although the improvement of skin and improvement of constipation were observed in the HAG diet, the effect of the improvement of the product of the present invention was remarkable, indicating that the composition of each compound material of the product of the present invention was excellent as a beauty food. ing. In addition, the intake of the beauty food of the product of the present invention is 0.5.
It is preferably from 02 to 20 g / kg · day.

As shown in Tests 1 to 5, the cosmetic food of the present invention is characterized in that hyaluronic acid is utilized by intestinal bacteria and is absorbed from the intestinal tract, anti-hyperlipidemia in serum, beneficial lactone in intestine For the first time, it was revealed that Bacillus bacteria increased,
The beauty effect was scientifically verified as well as a panelist's skin tactile test and constipation test. Although the low molecular weight of 500,000 or less has been considered appropriate from the viewpoint of availability, it has been found that hyaluronic acid can be sufficiently used in the intestine even with a molecular weight of about 1,000,000. Rather, the high molecular weight compounds were more effective at increasing the number of lactobacilli and bifidobacteria in the intestine than the low molecular weight ones.

Therefore, when the cosmetic food of the present invention is orally ingested, the synergistic effect of the components of hyaluronic acid, glucosamine, oligosaccharides and vitamin C makes it possible for the skin effect to be higher than the sum of the individual effects of the components. An excellent cosmetic action such as enhancing production of hyaluronic acid and collagen can be produced.

The beauty food of the present invention has a weight ratio of hyaluronic acid of 0.01 to 0.25, a weight ratio of glucosamine of 0.005 to 0.15, and a weight of oligosaccharide, relative to the total weight of 1. When the ratio is set to 0.02 to 0.35 and the weight ratio of vitamin C is set to 0.01 to 0.25, a cosmetic effect can be surely obtained. Here, when the weight ratio of each component to the total weight of 1 is larger than the above range, there is a problem that the cosmetic effect tends to decrease and the manufacturing cost increases. When the weight ratio of each component is less than the above range, the cosmetic effect is significantly reduced. Incidentally, the weight ratio of hyaluronic acid to 0.01
5 to 0.20, the weight ratio of glucosamine is 0.02 to 0.
10. When the weight ratio of the oligosaccharide is set to 0.05 to 0.30 and the weight ratio of vitamin C is set to 0.05 to 0.25, a particularly optimal cosmetic effect can be obtained.

The molecular weight of hyaluronic acid is increased from 600,000 to 200
When the amount is 10,000, lactobacilli and bifidobacteria in the intestine are increased as compared with those having a low molecular weight of 500,000 or less, so that the intestinal regulation action is enhanced and the cosmetic action can be further enhanced.

When hyaluronic acid is extracted from a cockscomb or produced by fermentation of microorganisms, high-pressure, high-temperature, and enzyme treatments conventionally required to reduce the molecular weight of hyaluronic acid to 500,000 or less are unnecessary, and the production cost is reduced. Can be reduced.

Glucosamine is converted to 2-amino-2-deoxy-
D-glucose or 2-amino-2-deoxy-D-glucose salt, wherein the oligosaccharide is at least one of maltooligosaccharide, galactooligosaccharide, fructooligosaccharide, isomaltooligosaccharide, gentiooligosaccharide, lactosucrose and trehalose With the above configuration, the cosmetic effect can be enhanced and the manufacturing cost can be further reduced.

The beauty food of the present invention is not limited to the above-described embodiment, and various functional materials may be added, and various modifications may be made without departing from the gist of the present invention. Can of course be added.

[0065]

According to the beauty food of the present invention, various excellent effects as described below can be obtained.

(I) When the cosmetic food of the present invention is orally ingested, it has excellent synergistic effects of components of hyaluronic acid, glucosamine, oligosaccharides and vitamin C, thereby enhancing the production of hyaluronic acid and collagen in the skin. Can produce cosmetic effects.

(II) When the molecular weight of hyaluronic acid is from 600,000 to 2,000,000, the number of lactobacilli and bifidobacteria in the intestine is increased as compared with those having a low molecular weight of 500,000 or less, so that the intestinal action is enhanced. Beauty action can be further enhanced.

(III) When hyaluronic acid is extracted from a cockscomb or produced by fermentation of microorganisms, high-pressure, high-temperature, and enzyme treatments conventionally required to reduce the molecular weight of hyaluronic acid to 500,000 or less become unnecessary. In addition, manufacturing costs can be reduced.

(IV) Glucosamine is converted to 2-amino-2-
Deoxy-D-glucose or 2-amino-2-deoxy-D-
When the oligosaccharide is composed of at least one of maltooligosaccharide, galactooligosaccharide, fructooligosaccharide, isomaltooligosaccharide, gentiooligosaccharide, lactosucrose, and trehalose, the cosmetic effect is enhanced and the production cost is further increased. Can be reduced.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme court ゛ (Reference) A61K 31/702 A61K 31/702 31/715 31/715 31/726 31/726 31/728 31/728 35 / 12 35/12 A61P 17/00 A61P 17/00 43/00 107 43/00 107 (72) Inventor Tomohiro Yamagata 45 Iwano, Ueki-cho, Kamoto-gun, Kumamoto F-term (reference) 4B018 LE01 MD16 MD25 MD33 MD41 ME14 MF01 MF10 4C086 AA01 AA02 BA18 EA01 EA02 EA20 EA22 EA25 MA03 MA04 MA52 NA05 NA10 ZA89 ZB22 4C087 AA01 AA02 AA03 BB33 MA52 NA05 NA10 ZA89 ZB22

Claims (7)

[Claims] 1. A beauty food comprising hyaluronic acid, glucosamine, oligosaccharides and vitamin C. 2. The weight ratio of hyaluronic acid is 0.01 to 0.25, the weight ratio of glucosamine is 0.005 to 0.15, and the weight ratio of oligosaccharide is 0.02 to 1 weight of the whole.
0.35, the weight ratio of vitamin C is 0.01-0.25
The beauty food according to claim 1, wherein 3. The molecular weight of hyaluronic acid is from 600,000 to 20
3. The beauty food according to claim 1 or 2, wherein the amount is set at 100,000. 4. The beauty food according to claim 1, wherein the hyaluronic acid is extracted from a cockscomb. 5. The method according to claim 5, wherein the hyaluronic acid is Streptococcus zoopid.
The cosmetic food according to claim 1, 2 or 3, which is produced by fermentation of at least one of emicus and Streptococcus equisimilis microorganisms. 6. The glucosamine comprises at least one of 2-amino-2-deoxy-D-glucose produced by hydrolyzing chitin, and a salt of 2-amino-2-deoxy-D-glucose. The beauty food according to 1, 2, or 3. 7. The cosmetic food according to claim 1, wherein the oligosaccharide comprises at least one of maltooligosaccharide, galactooligosaccharide, fructooligosaccharide, isomaltooligosaccharide, gentiooligosaccharide, lactosucrose, and trehalose.