JP2002199166A - Method for correcting jitter in two-way scan scanner, two-way scan scanner capable of jitter correction, and sample carrier - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a jitter correction method which easily corrects jitters in a two-way scan scanner at a low cost. SOLUTION: A sample for correction data generation where a regular pattern is formed is scanned by laser light; emitted light is detected photoelectrically to generate digital data for correction data generation, and data which minimizes the extent of bias of each scanning line of digital data for correction data generation is determined and stored as jitter correction data on the basis of digital data for correction data generation, and a marked sample is put on a sample stage and is scanned by laser light; and light emitted from the marked material is detected, photoelectrically and jitter correction data stored in a memory is used to perform correction.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for correcting jitter in a bidirectional scanning scanner, a bidirectional scanning scanner capable of correcting jitter, and a sample carrier, and more particularly, to a simple and low-cost scanner. Jitter correction method capable of correcting jitter in bidirectional scanning scanner, high resolution bidirectional scanning scanner capable of correcting jitter easily and at low cost, and simple and low cost The present invention relates to a sample carrier for a bidirectional scanning scanner capable of generating correction data for correcting jitter.

[0002]

2. Description of the Related Art When irradiated with radiation, the energy of the radiation is absorbed, stored, recorded, and then excited using electromagnetic waves in a specific wavelength range. A stimulable phosphor having a characteristic of emitting a stimulating amount of radiated light is used as a radiation detecting material, and a substance provided with a radioactive label is administered to an organism, and then the organism or a tissue of the organism is treated. Partly as a sample,
This sample is superimposed on a stimulable phosphor sheet provided with a stimulable phosphor layer for a certain period of time, whereby radiation energy is accumulated and recorded on the stimulable phosphor, and thereafter, the radiation energy is stimulated by electromagnetic waves. Scans the stimulable phosphor layer to excite the stimulable phosphor, photoelectrically detects the stimulable light emitted from the stimulable phosphor, generates a digital image signal, and performs image processing. An autoradiography detection system configured to reproduce an image on a display means such as chrome T or on a recording material such as a photographic film is known (for example, Japanese Patent Publication No. 1-60784, Japanese Patent Publication No. No. 1-60782, Japanese Patent Publication No. 4-3952, etc.).

An autoradiography detection system using a stimulable phosphor sheet as a material for detecting an image, unlike the case of using a photographic film, not only does not require a chemical treatment called a development process, but also obtains an obtained image. By performing image processing on image data, there is an advantage that an image can be reproduced or quantitative analysis can be performed by a computer as desired.

[0004] On the other hand, a fluorescence detection system using a fluorescent substance as a labeling substance instead of a radioactive labeling substance in an autoradiography system is known. According to this system, by reading a fluorescent image, gene sequence, gene expression level, separation and identification of protein, or evaluation of molecular weight and properties can be performed. After adding a fluorescent dye to the solution containing the fragments,
A plurality of DNA fragments are electrophoresed on a gel support, or a plurality of DNA fragments are placed on a gel support containing a fluorescent dye.
After the NA fragment is subjected to electrophoresis, or a plurality of DNA fragments are subjected to electrophoresis on a gel support, the gel support is immersed in a solution containing a fluorescent dye, etc. By labeling, exciting a fluorescent dye with excitation light, and detecting the generated fluorescence, an image is generated, and the distribution of DNA on the gel support is detected. After electrophoresis on a support, the DNA is denaturated, and then at least a part of the denatured DNA fragment is transferred onto a transfer support such as nitrocellulose by Southern blotting to obtain the desired DNA. A probe prepared by labeling DNA or RNA complementary to the DNA to be labeled with a fluorescent dye and a denatured DNA fragment is hybridized, and the probe DNA
Alternatively, only the DNA fragment complementary to the probe RNA is selectively labeled, and the excitation light excites the fluorescent dye,
By detecting the generated fluorescence, an image can be generated and the distribution of the target DNA on the transfer support can be detected. Further, a DNA probe complementary to the DNA containing the target gene labeled with the labeling substance is prepared, hybridized with the DNA on the transcription support, and the enzyme is reacted with the complementary DNA labeled with the labeling substance. After binding, it is brought into contact with a fluorescent substrate to convert the fluorescent substrate into a fluorescent substance that emits fluorescence, and the excitation light excites the generated fluorescent substance and detects the generated fluorescence to generate an image. However, the distribution of the target DNA on the transcription support can also be detected. This fluorescence detection system has an advantage that a gene sequence or the like can be easily detected without using a radioactive substance.

In recent years, hormones, tumor markers, enzymes, antibodies, antigens, abzymes,
Other proteins, nucleic acids, cDNA, DNA, RNA
Such as, a specific binding substance that can specifically bind to a substance derived from a living body, and has a known base sequence, base length, and composition, is dropped using a spotter apparatus, and a large number of independent binding substances are dropped. A spot is formed, and then, a hormone, a tumor marker, an enzyme, an antibody, an antigen, an abzyme, another protein, a nucleic acid, a cDNA, a DNA, an mRNA, or the like, is collected from a living body by extraction, isolation, or the like. Excitation light is applied to a microarray that is a substance derived from a living body that has been subjected to chemical treatment, chemical modification, etc., and that has been hybridized with a substance that is labeled with a labeling substance such as a fluorescent substance or dye. Substances, photoelectrically detect light such as fluorescence emitted from labeling substances such as dyes,
A microarray image detection system for analyzing a substance derived from a living body has been developed. According to this microarray image detection system, a large number of specific binding substance spots are formed at different positions on a carrier surface such as a slide glass plate or a membrane filter at a high density, and a biological substance labeled with a labeling substance is formed. By hybridizing the substance, there is an advantage that a substance derived from a living body can be analyzed in a short time.

[0006] In addition, hormones, tumor markers, enzymes, and the like can be located at different positions on the surface of a carrier such as a membrane filter.
Antibodies, antigens, abzymes, other proteins, nucleic acids,
Using a spotter, a specific binding substance that can specifically bind to a substance derived from a living body, such as cDNA, DNA, or RNA, and has a known base sequence, base length, or composition, is dropped using a spotter device. Form a number of independent spots, followed by hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNAs, DNs
A, mRNA, etc., a substance derived from a living body that has been collected from a living body by extraction, isolation, or the like, or that has been further subjected to a chemical treatment, a chemical modification, or the like, and that has been labeled with a radioactive labeling substance. The macroarray hybridized with the above is brought into close contact with the stimulable phosphor sheet on which the stimulable phosphor layer containing the stimulable phosphor is formed, and the stimulable phosphor layer is exposed, and then the luminescent phosphor layer is exposed. A macroarray detection system using a radioactive labeling substance that irradiates the stimulable phosphor layer with excitation light, photoelectrically detects the stimulable phosphor emitted from the stimulable phosphor layer, and analyzes substances derived from living organisms Has also been developed.

[0007]

SUMMARY OF THE INVENTION These systems are:
In each case, the sample is irradiated with excitation light to excite a labeled substance such as a stimulable phosphor or a fluorescent substance, and the stimulable phosphor emitted from the stimulable phosphor or the fluorescence emitted from the fluorescent substance is emitted. Is used to generate data for biochemical analysis such as image data and luminescence amount data of the labeling substance, and the data generator used for these systems is a scanner that uses a scanner. And two-dimensional sensors.

[0008] When a scanner is used, there is an advantage that data can be generated with high resolution as compared with the case where a two-dimensional sensor is used.

In this case, a bidirectional scanning scanner configured to reciprocate the excitation light in the main scanning direction with respect to the sample and scan the sample with the excitation light is used for biochemical analysis. Generating data is efficient, so it is common to use a bidirectional scanning scanner.

In such a bidirectional scanning scanner, the sample stage and the optical system are reciprocated at a high speed in the main scanning direction. There has been a problem that a variation occurs in a relative moving speed and a so-called jitter occurs in generated data due to a shift in data sampling timing.

In particular, in the case of a microarray system, on the surface of a slide glass plate or the like, fluorescence from a fluorescent substance labeling a biological substance hybridized with a specific binding substance is photoelectrically detected. When generating data, it is desirable to use a confocal optical system in order to improve the S / N ratio. For this purpose, it is necessary to reciprocate the stage on which the sample is placed in the main scanning direction. It is optically advantageous, and as a result, jitter is apt to occur remarkably, which is a serious problem.

Further, when the sample stage is configured to reciprocate in the main scanning direction by using a timing belt or the like for cost reduction, jitter is easily generated due to elongation of the timing belt and the like. The development of a method for effectively preventing the occurrence has been desired.

Therefore, the present invention provides a method for correcting jitter in a bidirectional scanning scanner which can be easily and inexpensively corrected.
A low-cost, high-resolution bi-directional scanning scanner capable of correcting jitter and a sample carrier for a bi-directional scanning scanner capable of easily and inexpensively generating correction data for correcting jitter. The purpose is to provide.

[0014]

SUMMARY OF THE INVENTION The object of the present invention is as follows.
The sample stage on which the sample is set and the laser light are relatively moved so as to reciprocate in the main scanning direction, and are relatively moved in a sub-scanning direction orthogonal to the main scanning direction. A method of correcting jitter in a bidirectional scanning scanner configured to scan the sample by the laser light and photoelectrically detect light emitted from the sample, with respect to the sample stage, A sample for correction data generation in which a relatively fixed and regular pattern is formed is scanned with the laser light, and light emitted from the sample for correction data generation is photoelectrically detected, and analog Generating data, digitizing the analog data, generating digital data for generating correction data, and generating the correction data. Based on the digital data, the data for minimizing the amount of deviation of the digital data for generating the correction data for each scan line is determined as jitter correction data, stored in the memory of the bidirectional scanning scanner, and On the stage, a sample labeled with a labeling substance is placed, and the laser beam scans the sample to excite the labeling substance,
The light emitted from the labeling substance is photoelectrically detected, analog data is generated, and digital data of a sample obtained by digitizing the analog data is stored in the memory of the bidirectional scanning scanner. This is achieved by a method for correcting jitter in a bidirectional scanning scanner, wherein the correction is performed using jitter correction data.

According to the present invention, the laser light is relatively moved in the main scanning direction so as to reciprocate in the main scanning direction, and is relatively moved in the sub-scanning direction orthogonal to the main scanning direction. The sample for correction data generation, which is relatively fixed to the sample stage to be formed and has a regular pattern, is scanned by a laser beam, and the light emitted from the sample for correction data generation is photoelectrically scanned. And generating analog data, digitizing the analog data, generating digital data for generating correction data including jitter and having a deviation for each scan line, based on the digital data for generating correction data. Thus, data that minimizes the amount of deviation of digital data for generating correction data for each scan line is determined as jitter correction data. Further, by correcting the digital data of the sample using the jitter correction data, it is possible to minimize the jitter in the digital data of the sample. According to the present invention, the jitter correction data thus obtained is The sample that is stored in the memory of the bidirectional scanning scanner is placed on the sample stage, and the sample that is labeled with the labeling substance is placed on the sample stage. Is photoelectrically detected, analog data is generated, and digital data of a sample obtained by digitizing the analog data is corrected using the jitter correction data stored in the memory. In order to improve the S / N ratio using a confocal optical system, the sample stage is moved in the main scanning direction. Even when reciprocated at a speed and in order to reduce costs, even in the case of using a timing belt, easily becomes a jitter in the digital data of the sample can be minimized.

In a preferred embodiment of the present invention, the sample stage and the laser beam are reciprocated once in the main scanning direction, and the sample for correction data generation is scanned by the laser beam. Then, light emitted from the sample for generating the correction data is photoelectrically detected, analog data is generated, the analog data is digitized, and digital data on the first line corresponding to the outward path and the return path are output. Generating digital data for correction data generation consisting of digital data of the second line corresponding to the digital data for the first line, based on the digital data for correction data generation, Data that minimizes the amount of deviation of digital data is determined as jitter correction data, The bidirectional scanning scanner is configured to correct the digital data of the sample corresponding to the even-numbered scan line using the jitter correction data stored in the memory of the bidirectional scanning scanner. ing.

According to a preferred embodiment of the present invention, the sample stage and the laser light are caused to reciprocate once in the main scanning direction, and the laser light is used to scan a sample for generating correction data, thereby correcting the correction data. The light emitted from the sample for data generation is photoelectrically detected, analog data is generated, the analog data is digitized, and the digital data of the first line corresponding to the forward path and the first line corresponding to the return path Generating digital data for generating correction data composed of the digital data of the first line, and minimizing a deviation amount of the digital data of the second line with respect to the digital data of the first line based on the digital data for generating the correction data The data is determined as jitter correction data, stored in the memory of the bidirectional scanning scanner, and The digital data of the sample corresponding to the even-numbered scan line is corrected by using the digital data of the second line with respect to the digital data of the first line. Data is determined as jitter correction data,
By using the jitter correction data to correct the digital data of the sample corresponding to the even-numbered scan lines, the jitter in the digital data of the sample can be minimized. S /
Even when the sample stage is reciprocated at high speed in the main scanning direction to improve the N ratio, or when a timing belt is used to reduce cost,
It is possible to simply minimize the jitter in the digital data of the sample.

In another preferred embodiment of the present invention, the sample stage and the laser beam are relatively moved in a main scanning direction and a sub-scanning direction, and the laser beam is used to move the sample stage and the laser beam. Scanning the sample, photoelectrically detecting light emitted from the sample for correction data generation, generating analog data, digitizing the analog data, generating digital data for correction data generation, On the basis of the digital data for generating the correction data, 2N to the digital data (N is an integer of 1 or more) of the (2N-1) th line
Data that minimizes the amount of deviation of the digital data of the second line is determined as jitter correction data, and is stored in the memory of the bidirectional scanning scanner;
The digital data of the sample corresponding to the 2Nth line is corrected using the jitter correction data stored in the memory of the bidirectional scanning scanner.

According to another preferred embodiment of the present invention,
The sample stage and the laser beam are relatively moved in the main scanning direction and the sub-scanning direction, and the laser beam scans the correction data generation sample, and the light emitted from the correction data generation sample is photoelectrically converted. , Analog data is generated, the analog data is digitized, digital data for generating correction data is generated, and (2N) is generated based on the digital data for generating correction data.
-1) Data that minimizes the deviation of the digital data of the 2Nth line with respect to the digital data of the 1st line (N is an integer of 1 or more) are respectively determined as jitter correction data, Since the digital data of the sample corresponding to the 2N-th line is stored in the memory and the jitter correction data is used to correct the digital data, the confocal optical system is used to correct
In a case where the sample stage is reciprocated at a high speed in the main scanning direction in order to improve the / N ratio, and also in a case where a timing belt is used in order to reduce the cost, the sample stage is simply and more easily. It is possible to minimize jitter in digital data of a sample with high accuracy.

In a further preferred aspect of the present invention, the sample stage and the laser beam are relatively moved in a sub-scanning direction at a large moving pitch, and the jitter correction data is transferred to four or more lines. Generating for each data area of the digital data of the sample including,
The bidirectional scanning scanner is configured to be stored in the memory.

According to a further preferred embodiment of the present invention, even when the generated jitter differs depending on the data area of the sample digital data, the jitter correction data is stored in the data area of the sample digital data including four or more lines. Each time the sample stage is generated and the digital data of the sample is corrected, the sample stage is reciprocated at high speed in the main scanning direction using a confocal optical system in order to improve the S / N ratio. In this case, and when a timing belt is used to reduce the cost, it is possible to easily and more accurately minimize the jitter in the digital data of the sample.

In a further preferred aspect of the present invention, the regular pattern formed on the correction data generation sample is formed of a fluorescent material, and the laser light excites the fluorescent material, The apparatus is configured to photoelectrically detect fluorescence emitted from the correction data generation sample and generate the correction data generation digital data.

In another preferred embodiment of the present invention, the regular pattern formed on the correction data generation sample is formed by a visible shading pattern and reflected by the correction data generation sample. The laser beam is photoelectrically detected to generate digital data for generating the correction data.

In a further preferred aspect of the present invention, the sample for correction data generation is scanned by the laser light by moving the sample stage in the sub-scanning direction while reciprocating in the main scanning direction. Detecting photoelectrically emitted light from the correction data generation sample, generating analog data, digitizing the analog data, generating digital data for correction data generation, and generating the correction data Data for minimizing the amount of deviation of the digital data for correction data generation for each scan line based on the digital data for
Determined as jitter correction data, stored in the memory of the bidirectional scanning scanner, the sample stage,
A sample labeled with a labeling substance is placed, and the sample stage is moved in the sub-scanning direction while reciprocating in the main scanning direction, and is moved by the laser light.
Scanning the sample, exciting the labeling substance, photoelectrically detecting light emitted from the labeling substance, generating analog data, and converting digital data of the sample obtained by digitizing the analog data. The bidirectional scanning scanner is configured to perform correction using the jitter correction data stored in the memory.

According to a further preferred embodiment of the present invention, even when a confocal optical system is used to improve the S / N ratio, it is possible to easily minimize the jitter in the digital data of the sample. Will be possible.

In a further preferred aspect of the present invention, the laser light is reciprocated in the main scanning direction while
Moved in the sub-scanning direction, scan the sample for correction data generation by the laser light, photoelectrically detect the light emitted from the sample for correction data generation, generate analog data, The analog data is digitized to generate digital data for generating correction data, and a deviation amount of the digital data for generating correction data for each scan line is minimized based on the digital data for generating correction data. Data is determined as jitter correction data, stored in the memory of the bidirectional scanning scanner, a sample marked with a labeling substance is placed on the sample stage, and the laser light is reciprocated in the main scanning direction. While moving, the sample is scanned by the laser beam while being moved in the sub-scanning direction, and the labeling substance is scanned. Excited, the light emitted from the labeling substance is photoelectrically detecting generates analog data,
The digital data of the sample obtained by digitizing the analog data is corrected using the jitter correction data stored in the memory of the bidirectional scanning scanner.

In a further preferred aspect of the present invention, jitter correction data is generated in accordance with the relative movement speed of the sample stage and the laser beam in the main scanning direction, respectively, and the jitter correction data is generated. It is configured to be stored in the memory.

According to a further preferred embodiment of the present invention, jitter correction data is respectively generated in accordance with the relative movement speed of the sample stage and the laser beam in the main scanning direction, and the jitter correction data is generated in the memory of the bidirectional scanning scanner. Since it is configured to be stored, it is possible to minimize the jitter in the digital data of the sample with higher accuracy.

In a further preferred aspect of the present invention, the sample stage and the laser beam are relatively moved in a main scanning direction at a specific moving speed to generate jitter correction data, The sample stage and the laser light were stored in the memory of the scanning scanner, and the sample stage and the laser beam were relatively moved in the main scanning direction at a moving speed different from the specific moving speed to generate digital data of the sample. When the jitter correction data stored in the memory of the bidirectional scanning scanner is corrected, jitter correction data is generated, and the digital data of the sample is corrected.

According to a further preferred embodiment of the present invention, the sample stage and the laser beam are relatively moved in the main scanning direction at a specific moving speed, to generate jitter correction data, and to perform bidirectional scanning. When digital data of a sample is generated by moving the sample stage and the laser beam in the main scanning direction at a moving speed different from a specific moving speed, and storing the sample stage and the laser beam in the memory of the scanner, the memory of the bidirectional scanning scanner is used. It is configured to correct the jitter correction data stored in the memory, generate the jitter correction data, and correct the digital data of the sample.The sample stage and the laser beam are moved at a specific moving speed in the main scanning direction. Jitter correction data is generated only when the camera is relatively moved, and when the relative movement speed is different, the data of the bidirectional scanning scanner is used. It corrects the jitter compensation data stored in the re, because to correct the jitter of the digital data samples, a simple, consisting of jitter in the digital data of the sample can be minimized.

In a further preferred aspect of the present invention, the sample stage and the laser beam generate jitter correction data in accordance with a pixel pitch when relatively moved in the main scanning direction, respectively. The bidirectional scanning scanner is configured to be stored in the memory.

According to a further preferred embodiment of the present invention, the sample stage and the laser beam respectively generate jitter correction data according to the pixel pitch when relatively moved in the main scanning direction. Since the data is stored in the memory of the counter-scanning scanner, the jitter in the digital data of the sample can be minimized with higher accuracy.

In a further preferred aspect of the present invention, the sample stage and the laser beam are relatively moved at a specific pixel pitch in the main scanning direction to generate jitter correction data, Stored in the memory of the scanning scanner, the sample stage and the laser light, in the main scanning direction, at a pixel pitch different from the specific pixel pitch, relatively moved, to generate digital data of the sample When the jitter correction data stored in the memory of the bidirectional scanning scanner is corrected, jitter correction data is generated, and the digital data of the sample is corrected.

According to a further preferred embodiment of the present invention, the sample stage and the laser beam are relatively moved in the main scanning direction at a specific pixel pitch to generate jitter correction data, and perform bidirectional scanning. The sample stage and the laser beam are stored in the memory of the scanner,
When digital data of a sample is generated by relatively moving at a pixel pitch different from a specific pixel pitch, the jitter correction data stored in the memory of the bidirectional scanning scanner is corrected, and the jitter correction data is converted. Generate
It is configured to correct the digital data of the sample, and generates the jitter correction data only when the sample stage and the laser beam are relatively moved in the main scanning direction at a specific pixel pitch. When the pitch is different, the jitter in the digital data of the sample is corrected by correcting the jitter correction data stored in the memory, so that the jitter in the digital data of the sample can be easily minimized. Become.

In a further preferred embodiment of the present invention, jitter correction data is generated as required and stored in the memory.

According to a further preferred embodiment of the present invention, even if the generated jitter differs due to the elongation of the timing belt being deviated with time, etc.
As appropriate, generate jitter correction data, store it in memory, and correct the digital data of the sample using the new jitter correction data, thereby minimizing jitter in the digital data of the sample with higher accuracy. It becomes possible.

In a further preferred embodiment of the present invention, a sample labeled with a fluorescent substance is placed on the sample stage, and the sample is scanned with a laser beam to excite the fluorescent substance. Fluorescence emitted from a fluorescent substance is photoelectrically detected, analog data is generated, and digital data of a sample obtained by digitizing the analog data is stored in the memory of the bidirectional scanning scanner. It is configured to perform correction using the correction data.

In a further preferred embodiment of the present invention, a stimulable phosphor sheet having a stimulable phosphor layer labeled with a radioactive label is placed on the sample stage as the sample, and a laser is provided. By scanning the stimulable phosphor layer with light, the stimulable phosphor contained in the stimulable phosphor layer is excited, and the stimulable phosphor emitted from the stimulable phosphor is photo-emitted. The digital data of a sample obtained by digitally detecting the analog data, digitizing the analog data, and correcting the digital data using the jitter correction data stored in the memory of the bidirectional scanning scanner. Is configured.

The object of the present invention also includes a sample stage on which a sample labeled with a labeling substance is set, and at least one laser excitation light source for emitting laser light, wherein the sample stage and the laser light are:
In the main scanning direction, they are relatively moved so as to reciprocate with each other, and are relatively moved in a sub-scanning direction orthogonal to the main scanning direction, and by the laser light,
A bidirectional scanning scanner configured to scan the sample and photoelectrically detect light emitted from the sample, further comprising photoelectrically receiving light emitted from the labeling substance, A photodetector that generates analog data, and the laser light emitted from the at least one laser excitation light source is focused on the sample stage, and light emitted from the labeling substance is transmitted to the photodetector. A condensing optical system for guiding, an A / D converter for digitizing analog data generated by the photodetector,
A data processing device, and a memory, wherein the data processing device is fixed relative to the sample stage and a sample for generating correction data in which a regular pattern is formed is the at least one laser. Scanning is performed by a laser beam emitted from an excitation light source, and light emitted from the correction data generation sample is photoelectrically detected by the photodetector to generate analog data. Based on the generated digital data for correction data generated by the converter, the data for minimizing the amount of deviation of the digital data for correction data generation for each scan line is determined as jitter correction data. The sample stored in the memory, placed on the sample stage, and labeled with the labeling substance is Scanning by laser light emitted from at least one laser excitation light source, the labeling substance is excited, light emitted from the labeling substance is photoelectrically detected by the photodetector, and analog data is converted. Digital data of the sample generated and digitized by the A / D converter,
The present invention is attained by a bidirectional scanning scanner configured to perform correction using the jitter correction data stored in the memory.

According to the present invention, the bidirectional scanning scanner comprises:
A sample stage on which a sample labeled by the labeling substance is set; and at least one laser excitation light source for emitting laser light. The sample stage and the laser light are relatively moved so as to reciprocate with each other in the main scanning direction. And moved relatively in the sub-scanning direction orthogonal to the main scanning direction, scans the sample with the laser light, and is configured to photoelectrically detect light emitted from the sample, A photodetector that photoelectrically receives light emitted from a labeling substance to generate analog data, and condenses laser light emitted from at least one laser excitation light source on a sample stage, Optical system that guides the light emitted from the detector to the photodetector and digitizes the analog data generated by the photodetector / D converter, and a data processing device,
A memory, and a data processing device scans the sample for correction data generation, which is fixed relative to the sample stage and has a regular pattern, by using a laser beam, and obtains a sample for correction data generation. The light emitted from the photodetector is photoelectrically detected by a photodetector, generates analog data, is digitized by an A / D converter, includes jitter, and has a bias for each scan line. Digital data is generated, and based on the digital data for generating the correction data, data for minimizing the deviation amount of the digital data for generating the correction data for each scan line is determined as jitter correction data and stored in the memory. The sample placed on the sample stage and labeled with the labeling substance is scanned by laser light to excite the labeling substance. And the light emitted from the labeling substance,
The photodetector photoelectrically detects and digitizes the generated analog data by an A / D converter, and corrects the digital data of the generated sample using the jitter correction data stored in the memory. In order to improve the S / N ratio using a confocal optical system, the cost can be reduced even when the sample stage is reciprocated at high speed in the main scanning direction. Therefore, even when a timing belt is used, it is possible to easily minimize the jitter in the digital data of the sample generated by the scanner.

In a preferred embodiment of the present invention, the data processing device is configured such that the sample stage and the laser light are reciprocated once in the main scanning direction, and the correction data generation is performed by the laser light. Sample is scanned, light emitted from the correction data generation sample is photoelectrically detected by the photodetector, and the generated analog data is digitally converted by the A / D converter. The digital data of the first line is generated based on the generated digital data of the first line corresponding to the outward path and the generated digital data of the first line corresponding to the return path. Data that minimizes the deviation of the digital data on the second line with respect to the data is determined as jitter correction data. , Together it is stored in the memory, by using the jitter compensation data, and is configured to correct the digital data of the sample corresponding to the even-numbered scanning lines.

According to a preferred embodiment of the present invention, the data processing device makes the sample stage and the laser beam reciprocate once in the main scanning direction relatively to each other, and
A sample for correction data generation is scanned, and light emitted from the sample for correction data generation is detected by a photodetector.
The analog data generated by photoelectrically detecting and generating the A / A
A first line is generated based on digital data for correction data generation, which is digitized by the D converter and is generated by digital data of the first line corresponding to the forward path and digital data of the second line corresponding to the return path. The data that minimizes the deviation of the digital data of the second line with respect to the digital data of the eye is determined as jitter correction data, stored in the memory of the scanner, and is used for the even-numbered scan line using the jitter correction data. The digital data of the corresponding sample is configured to be corrected, and data that minimizes the deviation of the digital data of the second line with respect to the digital data of the first line is determined as jitter correction data. Using the correction data, the samples corresponding to the even-numbered scan lines are Ijitarudeta only to compensate for,
Since the jitter in the digital data of the sample can be minimized, in order to improve the S / N ratio using a confocal optical system, the sample stage is required to reciprocate at high speed in the main scanning direction. In addition, when a timing belt is used to reduce cost, it is possible to easily minimize jitter in digital data of a sample.

In another preferred embodiment of the present invention, the data processing device is arranged such that the sample stage and the laser beam are relatively moved in the main scanning direction and the sub-scanning direction, and Is scanned, light emitted from the correction data generation sample is photoelectrically detected by the photodetector, and the generated analog data is digitized by the A / D converter. Based on the generated correction data generation digital data, the data minimizing the deviation of the 2N-th line digital data with respect to the (2N-1) -th line digital data (N is an integer of 1 or more) Are respectively determined as jitter correction data, and are stored in the memory. Th is configured to correct the digital data of the sample corresponding to the line.

According to another preferred embodiment of the present invention,
The data processing device relatively moves the sample stage and the laser light in the main scanning direction and the sub-scanning direction, scans the correction data generation sample, and emits light emitted from the correction data generation sample. The analog data generated by photoelectrically detected by the photodetector and digitized by the A / D converter is digitized by the A / D converter, and based on the generated digital data for generating correction data, the (2N-1) th Data that minimizes the deviation of the digital data of the 2Nth line with respect to the digital data of the line (N is an integer of 1 or more) is determined as jitter correction data, and is stored in the memory of the scanner, respectively. Since the digital data of the sample corresponding to the 2Nth line is corrected using the data, When the sample stage is reciprocated at high speed in the main scanning direction to improve the S / N ratio by using the confocal optical system, and when the timing belt is used to reduce the cost. In addition, it is possible to easily and more accurately minimize the jitter in the digital data of the sample.

[0045] In a further preferred aspect of the present invention, the data processing device relatively moves the sample stage and the laser beam in a sub-scanning direction at a large moving pitch, and stores the jitter correction data. And for each data area of the digital data of the sample including four or more lines, and is stored in the memory.

According to a further preferred embodiment of the present invention, even when the generated jitter differs depending on the data area of the digital data of the sample, the jitter correction data is stored in the data area of the digital data of the sample including four or more lines. Each time, the digital data of the sample is generated and corrected, so that the jitter in the digital data of the sample can be easily and more accurately minimized.

In a further preferred aspect of the present invention, the regular pattern formed on the sample for generating the correction data is formed of a fluorescent material, and the data processing device is configured to control the fluorescent light by the laser light. When the substance is excited, the fluorescence emitted from the sample for generating the correction data is photoelectrically detected to generate digital data for generating the correction data.

In still another preferred embodiment of the present invention, the regular pattern formed on the correction data generation sample is formed by a visible light and shade pattern, and the data processing device is configured to execute the correction data generation The laser beam reflected by the sample is photoelectrically detected to generate digital data for generating the correction data.

[0049] In a further preferred aspect of the present invention, the data processing device is configured to control the relative movement speed of the sample stage and the laser beam in the main scanning direction.
Each is configured to generate jitter correction data and store it in the memory.

According to a further preferred embodiment of the present invention, the data processing device generates the jitter correction data according to the relative moving speed of the laser beam in the main scanning direction with respect to the sample stage, and generates the jitter correction data. When the sample stage is reciprocated at a high speed in the main scanning direction in order to improve the S / N ratio by using the confocal optical system, Even when a timing belt is used to reduce the cost, it is possible to easily and more accurately minimize the jitter in the digital data of the sample.

In a further preferred aspect of the present invention, when the sample stage and the laser beam are relatively moved at a specific moving speed in the main scanning direction, the data processing device may perform jitter correction. Generate data,
Stored in the memory, the sample stage, in the main scanning direction, at a moving speed different from the specific moving speed,
When moved, when the digital data of the sample is generated, the data processing device corrects the jitter correction data stored in the memory to generate jitter correction data, and converts the digital data of the sample. It is configured to correct.

According to a further preferred embodiment of the present invention, the data processing apparatus generates the jitter correction data by relatively moving the sample stage and the laser beam at a specific moving speed in the main scanning direction. When digital data of a sample is generated by relatively moving the sample stage in the main scanning direction at a moving speed different from a specific moving speed, the sample stage is stored in the memory. The jitter correction data is corrected, jitter correction data is generated, and the digital data of the sample is corrected.The sample stage and the laser beam are relatively moved in the main scanning direction at a specific moving speed. Jitter correction data is generated only when the camera is moved, and when the relative movement speed is different, the jitter correction data stored in the memory is corrected. Since the jitter of the digital data of the sample is corrected, even when the sample stage is reciprocated at high speed in the main scanning direction in order to improve the S / N ratio by using the confocal optical system, Further, even when a timing belt is used to reduce the cost, it is possible to easily minimize the jitter in the digital data of the sample.

[0053] In a further preferred aspect of the present invention, the data processing device may perform jitter correction according to a pixel pitch when the sample stage and the laser beam are relatively moved in the main scanning direction. It is configured to generate data and store the data in the memory of the bidirectional scanning scanner.

According to a further preferred embodiment of the present invention, the data processing apparatus stores the jitter correction data according to the pixel pitch when the sample stage and the laser beam are relatively moved in the main scanning direction. When the sample stage is reciprocated at high speed in the main scanning direction in order to improve the S / N ratio by using the confocal optical system, it is configured to generate and store it in the memory. In addition, even when a timing belt is used to reduce costs, it is possible to easily and more accurately minimize jitter in digital data of a sample.

In a further preferred aspect of the present invention, when the sample stage and the laser beam are relatively moved at a specific pixel pitch in the main scanning direction,
The data processing apparatus generates jitter correction data, stores the jitter correction data in the memory,
When the digital data of the sample is generated by moving in the main scanning direction at a pixel pitch different from the specific pixel pitch and the digital data of the sample is generated, the data processing device converts the jitter correction data stored in the memory. The correction is performed to generate jitter correction data, and the digital data of the sample is corrected.

According to a further preferred embodiment of the present invention, the data processing apparatus generates the jitter correction data by relatively moving the sample stage and the laser beam at a specific pixel pitch in the main scanning direction. And store it in a memory, and the sample stage and the laser beam are
When digital data of a sample is generated by relatively moving at a pixel pitch different from a specific pixel pitch, the jitter correction data stored in the memory is corrected, and the jitter correction data is generated. The jitter correction data is generated only when the sample stage and the laser beam are relatively moved in the main scanning direction at a specific pixel pitch. Is different, the jitter correction data stored in the memory is corrected to correct the jitter of the digital data of the sample. Therefore, the confocal optical system is used to improve the S / N ratio. When the stage is reciprocated at high speed in the main scanning direction, or when a timing belt is used to reduce cost. Easily becomes a jitter in the digital data of the sample can be minimized.

In a further preferred aspect of the present invention, a regular pattern of the fluorescent substance is formed on the surface of the sample stage on the side of the at least one laser excitation light source, and the data processing apparatus Are configured to be able to update the jitter correction data stored in the memory.

According to a further preferred embodiment of the present invention, a regular pattern of a fluorescent substance is formed on the surface of the sample stage on the side of the at least one laser excitation light source. It is configured to be able to update the jitter correction data stored in the, so even if the generated jitter changes due to the elongation of the timing belt and changes over time, the jitter correction data can be Generate and store in memory and correct the digital data of the sample using the new jitter correction data, thereby improving the S / N ratio using confocal optics. Even when reciprocating at high speed in the main scanning direction, or when using a timing belt to reduce costs, simple And, with higher accuracy, so the jitter in the digital data of the sample can be minimized.

[0059] In a further preferred aspect of the present invention, the data processing device scans, with a laser beam, a sample mounted on the sample stage as the sample and labeled with a fluorescent substance, thereby obtaining the fluorescence. Exciting the substance, the fluorescence emitted from the fluorescent substance,
The analog data generated by photoelectrically detected by the photodetector is digitized by the A / D converter, and the generated digital data of the sample is converted into the jitter correction data stored in the memory. Is configured to perform correction.

[0060] In still another preferred embodiment of the present invention, the data processing device comprises:
The stimulable phosphor layer of the stimulable phosphor sheet provided with the stimulable phosphor layer placed on the sample stage and labeled with a radioactive labeling substance is scanned by a laser beam, and the stimulable phosphor layer is scanned. Exciting the stimulable phosphor contained in the stimulable phosphor layer, the stimulable light emitted from the stimulable phosphor is photoelectrically detected by the photodetector, and the generated analog data is output. The digital data of the sample generated by digitizing by the A / D converter is corrected by using the jitter correction data stored in the memory.

[0061] In a further preferred aspect of the present invention, the data processing apparatus is set on the sample stage, and has a regular pattern formed on the surface of a sample carrier having a sample holding section for holding the sample. Scanning with laser light emitted from the at least one laser excitation light source, and light emitted from the correction data generation sample is photoelectrically detected by the photodetector to generate analog data. Then, based on the generated digital data for correction data generated by the A / D converter, data for minimizing the amount of deviation of the digital data for correction data generation for each scan line, The data is determined as jitter correction data and stored in the memory.

According to a further preferred embodiment of the present invention, the data processing device is set on the sample stage and has at least a regular pattern formed on the surface of the sample carrier provided with the sample holder for holding the sample. Scanning is performed by laser light emitted from one laser excitation light source, light emitted from a sample for generating correction data is photoelectrically detected by a photodetector, and analog data is generated, and A / D is generated. Based on the digital data generated by the converter and generated for the correction data, the data for minimizing the deviation of the digital data for the correction data for each scan line is determined as the jitter correction data. , The timing belt elongation changes over time. Responsible for Runado also jitter occurs becomes different as appropriate, very easily,
In order to improve the S / N ratio by using a confocal optical system by generating jitter correction data, storing the jitter correction data in a memory, and correcting the digital data of the sample using the new jitter correction data, Even when the sample stage is reciprocated at high speed in the main scanning direction, or when a timing belt is used to reduce cost, the digital data of the sample can be easily and more accurately recorded. Can be minimized.

[0063] In a further preferred aspect of the present invention, the regular pattern is formed outside the sample holding portion of the sample carrier.

According to a further preferred embodiment of the present invention, since the regular pattern is formed outside the sample holding portion of the sample carrier, the sample held by the sample holding portion is scanned by the laser beam. In this case, light can be emitted from the correction data generation sample that forms a regular pattern to prevent noise from being generated in the data, thereby minimizing jitter in the digital data of the sample. Will be possible.

The object of the present invention is also a sample carrier provided with a sample holding portion capable of holding a sample, the sample carrier being mounted on a sample stage, wherein a regular pattern is formed on the surface on the sample stage side. This is achieved by a sample carrier characterized in that:

According to the present invention, the sample carrier provided with the sample holding portion capable of holding the sample and configured to be mountable on the sample stage has a structure in which the surface of the sample stage side is irradiated with excitation light. Is excited,
Since it has a regular pattern of a substance that emits fluorescence, the sample stage is moved as necessary, and the regular pattern is scanned by a laser beam, and the photo-electric light emitted from the regular pattern is scanned. And generating analog data, digitizing the analog data, generating digital data for generating correction data including jitter and having a deviation for each scan line, based on the digital data for generating correction data. Thus, data that minimizes the amount of deviation of digital data for generating correction data for each scan line can be determined as jitter correction data, and the jitter correction data thus obtained is stored in the memory of the bidirectional scanning scanner. The sample stage is held by holding the sample labeled with the labeling substance in the sample holding section. The sample is moved by scanning the laser beam to excite the labeling substance, the light emitted from the labeling substance is photoelectrically detected, analog data is generated, and the analog data is digitized. Digital data can be corrected by using the jitter correction data stored in the memory of the bidirectional scanning scanner to minimize the jitter. Therefore, the S / N ratio can be improved by using a confocal optical system. Therefore, even when the sample stage is reciprocated at a high speed in the main scanning direction, or when a timing belt is used to reduce cost, the sample stage can be easily and more accurately sampled. Jitter in digital data can be minimized.

In a preferred embodiment of the present invention, the regular pattern is formed outside the sample holding section of the sample carrier.

According to a preferred embodiment of the present invention, since the regular pattern is formed outside the sample holder of the sample carrier, the sample held by the sample holder is scanned by the laser beam. In this case, light is emitted from the correction data generation sample that forms a regular pattern, preventing noise from being generated in the data and minimizing jitter in the digital data of the sample become.

In a further preferred aspect of the present invention, the regular pattern is formed of a fluorescent substance. In yet another preferred embodiment of the present invention, the regular pattern is formed by a visible shading pattern.

[0070]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Preferred embodiments of the present invention will be described below in detail with reference to the accompanying drawings.

FIG. 1 is a schematic perspective view of a bidirectional scanning scanner according to a preferred embodiment of the present invention. The bidirectional scanning scanner according to this embodiment excites a labeling substance contained in a sample, and Detecting the light emitted from the
It is configured to generate data for biochemical analysis.

As shown in FIG. 1, the bidirectional scanning scanner according to the present embodiment includes a first laser excitation light source 1 that emits a laser beam 4 having a wavelength of 640 nm and a second laser excitation light source 1 that emits a laser beam 4 having a wavelength of 532 nm. 2 laser excitation light sources 2 and 4
A third laser excitation light source 3 that emits laser light 4 having a wavelength of 73 nm. In the present embodiment, the first laser excitation light source is constituted by a semiconductor laser light source, and the second laser excitation light source 2 and the third laser excitation light source 3 are configured to generate a second harmonic (Second Harmonic Generatio).
n) Consists of elements.

The laser light 4 generated by the first laser excitation light source 1 is collimated by a collimator lens 5 and then reflected by a mirror 6. In the optical path of the laser light 4 emitted from the first laser excitation light source 1 and reflected by the mirror 6, the first dichroic mirrors 7 and 532nm transmitting the 640nm laser light 4 and reflecting the 532nm wavelength light are provided. A second dichroic mirror 8 that transmits light having the above wavelength and reflects light having a wavelength of 473 nm is provided. The laser light 4 generated by the first laser excitation light source 1 is used as a first dichroic mirror. The light passes through 7 and the second dichroic mirror 8 and enters the optical head 15.

On the other hand, the laser light 4 generated from the second laser excitation light source 2 is collimated by the collimator lens 9 and then reflected by the first dichroic mirror 7 to change its direction by 90 degrees. Then, the light passes through the second dichroic mirror 8 and enters the optical head 15.

The laser light 4 generated from the third laser excitation light source 3 is collimated by the collimator lens 10 and then reflected by the second dichroic mirror 8 to change its direction by 90 degrees. After that, the light enters the optical head 15.

The optical head 15 includes a mirror 16, a perforated mirror 18 having a hole 17 formed in the center thereof, and a lens 19. The laser beam 4 incident on the optical head 15 is Reflected and perforated mirror 18
The sample carrier 21 set on the sample stage 20 through the hole 17 and the lens 19 formed in
Incident on top. Here, the sample stage 20 is configured to be movable in the X and Y directions in FIG. 1 by a scanning mechanism (not shown in FIG. 1).

The bidirectional scanning scanner according to this embodiment uses a microarray in which a glass slide plate is used as a carrier and a number of spots of a sample selectively labeled with a fluorescent dye are formed on the glass slide plate. It is configured to scan with light 4 to excite the fluorescent dye, photoelectrically detect the fluorescence emitted from the fluorescent dye, and generate data for biochemical analysis. A fluorescent sample using a transfer support containing a labeled denatured DNA as a carrier is scanned by a laser beam 4 to excite the fluorescent dye, and the fluorescence emitted from the fluorescent dye is photoelectrically detected to perform biochemical analysis. Such as a membrane filter, which is configured to generate data for The body is brought into close contact with the stimulable phosphor sheet on which the stimulable phosphor layer containing the stimulable phosphor is formed, and the position information of the radiolabeled substance obtained by exposing the stimulable phosphor layer is recorded. The stimulable phosphor layer of the stimulable phosphor sheet is scanned by the laser beam 4 to excite the stimulable phosphor and photoelectrically detect the stimulable light emitted from the stimulable phosphor. Thus, it is configured to be able to generate data for biochemical analysis.

A microarray using a slide glass plate as a carrier is produced, for example, as follows.

First, the surface of the slide glass plate was
Pretreated with an L-lysine solution or the like, and then, at a predetermined position on the surface of the slide glass plate, cDNAs, which are a plurality of specific binding substances having different base sequences and different from each other.
Is dropped using a spotter device.

On the other hand, RNA as a specimen is extracted from living cells, and RNA having poly A at the 3 ′ end is extracted from the RNA.
Extract the RNA. When synthesizing cDNA from the mRNA having poly A at the end, the probe DNA labeled with Cy-5 is generated in the presence of Cy-5 (registered trademark) as a labeling substance.

The probe DNA labeled with Cy-5 thus obtained is prepared into a predetermined solution, and gently placed on the surface of a slide glass onto which cDNA as a specific binding substance has been dropped, and hybridized.

FIG. 2 is a schematic perspective view of the microarray 22 thus obtained. In FIG. 2, reference numeral 23 denotes the dropped cDNA.

On the other hand, modified D labeled with a fluorescent dye
The NA electrophoresis image is recorded on the transfer support, for example, as follows.

That is, first, a plurality of DNA fragments including a DNA fragment comprising a target gene are separated and developed by electrophoresis on a gel support medium, and denatured by alkali treatment. Main chain D
NA.

Next, the gel support medium and the transfer support are overlaid by a known Southern blotting method, and at least a part of the denatured DNA fragment is transferred onto the transfer support, followed by heating treatment and ultraviolet irradiation. To fix.

Thereafter, a probe prepared by labeling DNA or RNA complementary to the DNA of the gene of interest with a fluorescent dye and the denatured DNA fragment on the transcription support 12 are
It is hybridized by heating treatment and double-stranded DN
A formation (renaturation) or formation of a DNA / RNA conjugate. Then, for example, using a fluorescent dye such as fluorescein, rhodamine, and Cy-5,
DNA complementary to the DNA of the target gene
Alternatively, the RNA is labeled to prepare a probe. At this time, since the denatured DNA fragment on the transcription support is fixed, only the DNA fragment complementary to the probe DNA or the probe RNA hybridizes to capture the fluorescently labeled probe. Thereafter, by washing away the non-hybridized probe with an appropriate solution,
On the transcription support, only the DNA fragment having the target gene forms a hybrid with the fluorescently labeled DNA or RNA, and is labeled with the fluorescent label. The thus obtained transfer support is provided with a modified D labeled with a fluorescent dye.
An electrophoretic image of NA is recorded.

On the other hand, in the stimulable phosphor layer of the stimulable phosphor sheet, for example, the positional information of the radioactive labeling substance is recorded as follows.

The surface of a carrier such as a membrane filter is pre-treated, and cDNAs, which are a plurality of specific binding substances whose base sequences are different from each other, are deposited at predetermined positions on the surface of the carrier such as a membrane filter. Use the device to drip.

On the other hand, RNA as a specimen is extracted from living cells, and RNA having poly A at the 3 ′ end is extracted from the RNA.
Extract the RNA. When synthesizing cDNA from the mRNA having poly A at the end, the probe DNA is labeled with the radiolabeled substance in the presence of a radiolabeled substance.

The probe DNA labeled with the radiolabeled substance thus obtained is prepared into a predetermined solution, and is gently placed on a carrier surface such as a membrane filter onto which cDNA as a specific binding substance has been dropped, and hybridized. .

Next, the stimulable phosphor layer formed on the stimulable phosphor sheet is superimposed on the surface of a carrier such as a membrane filter on which the hybridized sample is formed, and is kept in close contact for a predetermined time. By doing so, at least part of the radiation emitted from the radiolabeled substance on the carrier such as a membrane filter is absorbed by the stimulable phosphor layer formed on the stimulable phosphor sheet, and the position information of the radiolabeled substance is obtained. Is recorded in the stimulable phosphor layer.

When the laser beam 4 is incident on the sample 22 from the optical head 15, the sample beam 22 is generated by the laser beam 4 when the sample 22 is a microarray or a fluorescent sample.
The fluorescent substance is excited to emit fluorescence, and when the sample 22 is a stimulable phosphor sheet, the stimulable phosphor contained in the stimulable phosphor layer is excited to stimulate the stimulable phosphor. Is issued.

The fluorescence or stimulating light 25 emitted from the sample 22 is converted into parallel light by the lens 19 of the optical head 15 and reflected by the perforated mirror 17.
Any one of the filters 28a, 28a, 28b, 28c, 28d of the filter unit 27 having four filters 28a, 28b, 28c, 28d.
The light is incident on 28b, 28c, 28d.

The filter unit 27 is configured to be movable in the left-right direction in FIG. 1 by a motor (not shown), and predetermined filters 28a, 28b, 28c, 28d are provided depending on the type of laser excitation light source used. , Or in the optical path of the fluorescent or stimulating light 25.

Here, the filter 28a is a filter used when the first laser excitation light source 1 is used to excite the fluorescent substance contained in the sample 22 and read the fluorescence, and has a wavelength of 640 nm. Cut the light, 640n
It has the property of transmitting light having a wavelength longer than m.

The filter 28b is a filter used to excite the fluorescent dye contained in the sample 22 by using the second laser excitation light source 2 and to read the fluorescence. 532nm
It has the property of transmitting light with a longer wavelength than that.

Further, the filter 28c is a filter used when the third laser excitation light source 3 is used to excite the fluorescent dye contained in the sample 22 and read out the fluorescence, and the light having the wavelength of 473 nm is used. And cut 473n
It has the property of transmitting light having a wavelength longer than m.

When the sample 22 is a stimulable phosphor sheet, the filter 28d uses the first laser excitation light source 1 to excite the stimulable phosphor contained in the stimulable phosphor sheet. A filter used to read the stimulable light emitted from the stimulable phosphor, and transmits only light in the wavelength region of the stimulable light emitted from the stimulable phosphor;
It has the property of cutting light having a wavelength of 0 nm.

Therefore, these filters 28a, 28a, and 28a are used in accordance with the type of the laser excitation light source to be used, that is, the type of the sample and the type of the fluorescent substance labeling the sample.
By selectively using 8b, 28c, 28d,
It becomes possible to cut off light in a wavelength region that becomes noise.

The filter 28a of the filter unit 27,
After passing through 28b and 28c and cutting light in a predetermined wavelength range, the fluorescence or stimulating light 25 enters a mirror 29, is reflected, and is condensed by a lens 30.

The lens 19 and the lens 30 constitute a confocal optical system. As described above, the confocal optical system is employed because, when the sample 22 is a microarray using a slide glass plate as a carrier, the fluorescence emitted from a minute spot-shaped sample formed on the slide glass plate is used. To
This is to enable reading at a high S / N ratio.

At the focal position of the lens 30, a confocal switching member 31 is provided.

FIG. 3 is a schematic front view of the confocal switching member 31.

As shown in FIG. 3, the confocal switching member 31 has a plate shape and has three pinholes 3 having different diameters.
2a, 32b and 32c are formed.

The pinhole 32a having the smallest diameter is arranged in the optical path of the fluorescent light emitted from the microarray when the sample 22 is a microarray using a slide glass plate as a carrier. Reference numeral 32c denotes a sample arranged on the optical path of the fluorescence emitted from the transfer support when the sample 22 is a fluorescent sample using the transfer support as a carrier.

The pinhole 32b having an intermediate diameter
In the case where the sample 22 is a stimulable phosphor sheet, the sample 22 is arranged on the optical path of the stimulable light emitted from the stimulable phosphor layer.

As described above, at the position of the focal point of the lens 30,
By providing the confocal switching member 31, when the sample 22 is a microarray using a slide glass plate as a carrier,
The pinhole 32a having the smallest diameter is positioned in the optical path of the fluorescent light. When the sample 22 is a microarray using a slide glass plate as a carrier, the fluorescent dye is excited by the laser light 4 so that the fluorescent light is Since the light is emitted from the surface of the slide glass plate and the light emitting point is almost constant in the depth direction, it is necessary to form an image on the pinhole 32a having a small diameter using a confocal optical system in order to improve the S / N ratio. Is desirable.

On the other hand, when the sample 22 is a fluorescent sample using a transfer support as a carrier, the pinhole 32c having the largest diameter is located in the optical path of the fluorescent light.
When the sample 22 is a fluorescent sample using a transfer support as a carrier, when the fluorescent dye is excited by the laser beam 4, the fluorescent dye is distributed in the depth direction of the gel support, and Since the point fluctuates in the depth direction, the confocal optical system cannot form an image on a pinhole with a small diameter.Using a pinhole with a small diameter cuts the fluorescence emitted from the sample, This is because a sufficient signal intensity cannot be obtained when photoelectrically detected is used, and it is necessary to use the pinhole 32c having a large diameter.

On the other hand, when the sample 22 is a stimulable phosphor sheet, the pinhole 32b having an intermediate diameter is located in the optical path of the stimulable phosphor by the laser light 4. When the stimulable phosphor contained in the layer is excited, the emission points of the stimulable phosphor are distributed in the depth direction of the stimulable phosphor layer, and the emission points fluctuate in the depth direction. Due to the optical system, it is not possible to form an image on a pinhole with a small diameter, and if a pinhole with a small diameter is used, the photostimulable light emitted from the sample is cut, and when the photostimulable light is detected photoelectrically. Although a sufficient signal intensity cannot be obtained, the distribution in the depth direction of the light-emitting points and the fluctuation in the depth direction of the light-emitting points are not as large as those of the microarray using the gel support as a carrier. This is because it is desirable to use the hole 32b.

The fluorescence or stimulating light that has passed through the confocal switching member 31 is photoelectrically detected by the photomultiplier 33, and analog data is generated.

The analog data generated by the photomultiplier 33 is converted into digital data by the A / D converter 34 and sent to the data processing device 35.

FIG. 4 is a schematic perspective view showing details of the main scanning mechanism among the scanning mechanisms of the sample stage 20.

As shown in FIG. 4, a pair of guide rails 41 are placed on a movable substrate 40 movable in the sub-scanning direction indicated by an arrow Y in FIG. 4 by a sub-scanning motor (not shown). , 41 are fixed, and the sample stage 20 includes three slide members 42, 42 slidably mounted on a pair of guide rails 41, 41.
(Only two are shown in FIG. 4).

As shown in FIG. 4, a main scanning motor 43 is fixed on a movable substrate 40, and a timing belt wound around a pulley 44 is mounted on an output shaft 43 a of the main scanning motor 43. 45 is wound, and a rotary encoder 46 is attached.

Therefore, by driving the main scanning motor 43, the sample stage 20 is reciprocated along the pair of guide rails 41, 41 in the main scanning direction indicated by the arrow X in FIG. By moving the movable substrate 40 in the sub-scanning direction by a sub-scanning motor (not shown), the sample stage 20 is two-dimensionally moved, and the entire surface of the sample 22 set on the sample stage 20 is subjected to laser irradiation. The light 4 makes it possible to scan.

Here, the position of the sample stage 20 is
The configuration is such that monitoring can be performed by the rotary encoder 46.

FIG. 5 is a schematic perspective view of a sample carrier 21 which holds a microarray using a slide glass plate as a carrier and is set on a sample stage 20. It is the drawing seen from the mounting side.

As shown in FIG. 5, the sample carrier 21 has a frame body 50 formed by processing one plate-like member, and the sample body 22 is set inside the frame body 50. 5 possible openings 5
1, 52, 53, 54 and 55 are formed.

On the surface of the frame body 50 on both sides of each of the openings 51, 52, 53, 54, 55, rectangular plate members 60, 61, 62, 63, 64, 65 are respectively provided.
The side regions on the side of the openings 51, 52, 53, 54, 55 are placed on the openings 51, 52, 53, 54, 55 along the longitudinal direction of the openings 51, 52, 53, 54, 55. It is mounted so as to protrude.

As shown in FIG. 5, each opening 51, 5
L-shaped leaf springs 5 are provided in 2, 53, 54 and 55.
1 a, 52 a, 53 a, 54 a, 55 a are attached to the back side of the sample carrier 21 so that a spring force can be applied.
Each of the openings 51, 52, 5
The leaf springs 51b, 5b align the sample 22 set in 3, 54, 55 along the other opposing inner wall.
2b, 53b, 54b and 55b are attached.

The sample carrier 21 includes a frame body 50.
Are placed on the sample stage 20 and set on the sample stage 20.

When the microarray using the slide glass plate as the carrier as the sample 22 is set on the sample carrier 21, the sample 22 is placed in the direction indicated by the arrow A in FIG. 53, 5
4, 55.

On one inner wall of each of the openings 51, 52, 53, 54, 55, leaf springs 51b, 52b, 53b, 5b are provided.
4b and 55b are attached, the sample 22
Are aligned along the other opposing inner wall in each of the openings 51, 52, 53, 54, 55.

At the same time, the openings 51, 52, 53, 5
The bent portions of the L-shaped leaf springs 51a, 52a, 53a, 54a, and 55a abut on the sample 22 inserted into the sample springs 4 and 55, and the leaf springs 51a, 52a, 53a and 54
Due to the spring force of the openings a, 55a, the side regions on the opening 51, 52, 53, 54, 55 side respectively extend along the longitudinal direction of the openings 51, 52, 53, 54, 55. , Plate members 60, 6 attached to project over openings 51, 52, 53, 54, 55.
1, 62, 63, 64, and 65 are urged to the surface and held on the sample carrier 21.

In the sample carrier 21 shown in FIG. 5, the plate members 60, 61,
62, 63, 64, 65 have openings 51, 52, 5,
The side regions on the side of 3, 54, 55 have openings 51, 52, 5
Along the longitudinal direction of 3, 54, 55, openings 51, 5
2, 53, 54, 55 so as to protrude therefrom, and the sample 22 includes leaf springs 51 a, 52 a, 53 a,
Each of the plate members 6 by the spring force of
It is configured to be urged against the surfaces of 0, 61, 62, 63, 64, 65 and held on the sample carrier 21.

On the other hand, the sample carrier 21 has a frame 50 made by processing one plate-like member.
Are placed on the sample stage 20 and set on the sample stage 20.

Therefore, the surface of the plate members 60, 61, 62, 63, 64, and 65 on which the sample 22 is supported, and the sample carrier 21
The surfaces supported by are always in the same plane, so that the five samples 22 are always moved relative to the sample stage 20 without the complicated operation of adjusting the position of the sample carrier 21. With the same positional relationship, it is possible to set.

Further, the frame body 50 is simply made by processing one plate-like member, and the plate members 60, 61, 62, 63,
By simply mounting the 64 and 65 on the surface of the frame body 50, the five samples 22 can always be set in the same positional relationship with respect to the sample stage 20, so that the cost of the sample carrier 21 is greatly reduced. It becomes possible to reduce to.

FIG. 6 is a block diagram showing a detection system, a drive system, an input system, and a control system of a bidirectional scanning scanner according to a preferred embodiment of the present invention.

As shown in FIG. 6, the detection system of the bidirectional scanning scanner includes a carrier sensor 70 for detecting the type of carrier holding the sample 22 set on the sample stage 20.

As shown in FIG. 6, the drive system of the bidirectional scanning scanner includes a filter unit motor 71 for moving the filter unit 27, a switching member motor 72 for moving the confocal switching member 31, and a sample stage 20. Sub-scanning motor 43 for moving in the main scanning direction
And a sub-scanning motor 47 for moving the sample stage 20 in the sub-scanning direction.

As shown in FIG. 6, the input system of the bidirectional scanning scanner includes a keyboard 73, and the control system of the bidirectional scanning scanner includes a control unit 7
5 is provided.

As shown in FIG. 4, the bidirectional scanning scanner according to this embodiment is configured such that the sample stage 20 is reciprocated at a high speed in the main scanning direction by the main scanning motor 43. Generally, in such a bidirectional scanning scanner, the relative movement speed varies between the forward path and the return path due to the mechanical accuracy of the scanning mechanism, the load being moved, and the like. It is known that there is a problem that so-called jitter occurs in the generated data due to the timing deviation.

In the bidirectional scanning scanner according to this embodiment, the sample stage 20 is reciprocated at a high speed in the main scanning direction by the main scanning motor 43 via the timing belt 45. Jitter tends to occur due to belt elongation and the like.

Therefore, in the present embodiment, jitter correction data for correcting the jitter is generated in advance, stored in a memory (not shown) of the data processing device 35, and the laser light 4 To excite the fluorescent dye or stimulable phosphor, photoelectrically detect the fluorescence or stimulable light emitted from the fluorescent dye or stimulable phosphor, and digitize the digital data obtained. It is configured to be able to generate digital data that has been corrected using jitter correction data to minimize jitter.

FIG. 7 is a schematic front view of a correction data generation sample used when generating jitter correction data for correcting jitter.

As shown in FIG. 7, the correction data generating sample 80 has a regular basic pattern.

In this embodiment, the correction data generation sample 80 is formed of a color glass filter, and the surface of the color glass filter has a chromium vapor deposited film 81 formed thereon.
Are formed, and a regular basic pattern of a color glass filter is formed in a portion where the chromium deposition film 81 is not formed.

In this embodiment, the following is performed.
Jitter correction data is generated.

First, a correction data generation sample 80 is set on the sample stage 20 in place of the sample carrier 21.

Next, when the operator inputs the correction data generation sample scanning signal to the keyboard 73, the correction data generation sample scanning signal is output to the control unit 75.

Upon receiving the correction data generation sample scanning signal, the control unit 75 outputs a drive signal to the filter unit motor 71 so that the filter unit 2 is positioned in the optical path of the fluorescent light 25 so that the filter 28 a is positioned in the optical path of the fluorescent light 25.
7 and a drive signal is output to the switching member motor 72 to move the confocal switching member 31 so that the pinhole 32a is positioned in the optical path of the fluorescence 25.

The correction data generation sample scanning signal is sent from the control unit 75 to the data processing device 3 at the same time.
5 is output.

Next, the control unit 75 activates the first laser excitation light source 1 and emits the laser light 4 of 640 nm.

The laser light 4 emitted from the first laser excitation light source 1 is converted into parallel light by a collimator lens 5, then reflected by a mirror 6, and is reflected by a first dichroic mirror 7 and a second dichroic mirror. 8 and enter the optical head 15.

The laser beam 4 incident on the optical head 15 is
The light is reflected by the mirror 16, passes through the hole 17 formed in the perforated mirror 18, is condensed by the lens 19, and enters the correction data generation sample 80 set on the sample stage 20.

In this embodiment, the correction data generation sample 80 is formed of a color glass filter, and the surface of the color glass filter has a chromium vapor deposited film 81 formed thereon.
Is formed, and a regular basic pattern of a color glass filter is formed in a portion where the chromium vapor-deposited film 81 is not formed. Therefore, the chromium vapor-deposited film 81 is not formed, and a regular basic pattern is formed. The surface of the color glass filter is excited by the laser light 4 and the fluorescence 25 is emitted.

The fluorescent light 25 emitted from the surface of the color glass filter forming the regular basic pattern is converted into parallel light by the lens 19, reflected by the perforated mirror 18, and incident on the filter unit 27. I do.

The filter unit 27 includes a filter 28a
Has been moved so as to be located in the optical path,
5 enters the filter 28a, cuts light having a wavelength of 640 nm, and transmits only light having a wavelength longer than 640 nm.

The fluorescence 25 transmitted through the filter 28a is reflected by the mirror 29 and is imaged by the lens 30.

Prior to the irradiation of the laser beam 4, the confocal switching member 31 is moved so that the pinhole 32a having the smallest diameter is located in the optical path, so that the fluorescent light is focused on the pinhole 32a. , Photomultiplier 33
Is detected photoelectrically, and analog data is generated.

As described above, the fluorescence emitted from the surface of the color glass filter is guided to the photomultiplier 33 by using the confocal optical system and is photoelectrically detected, so that noise in data is minimized. It becomes possible to suppress.

The analog data generated by the photomultiplier 33 is converted into digital data by the A / D converter 34 and sent to the data processing device 35.

As described above, the sample stage 20
The main scanning motor 43 reciprocates at high speed in the main scanning direction indicated by an arrow X in FIG.
7 so as to be intermittently moved in the sub-scanning direction indicated by arrow Y.
7, the correction data generation sample 80, that is, the sample stage 20, is moved from right to left in FIG. 7, and the surface of the correction data generation sample 80 is scanned by the laser light 4 from left to right. .

As a result, the correction data generation sample 80
According to the regular basic pattern formed in the above, the color glass filter is scanned from left to right by the laser beam 4, the color glass filter is excited, and the fluorescence 25 emitted from the surface of the color glass filter is emitted. The analog data of the regular basic pattern detected photoelectrically by the photomultiplier 33 and formed on the correction data generation sample 80 is generated.

The analog data generated by the photomultiplier 33 is converted into digital data by the A / D converter 34, and the digital data of the first line of the regular basic pattern is converted into the digital data.
5 is output.

In FIG. 7, the correction data generation sample 80, that is, the sample stage 20, is moved by the main scanning motor 43 from right to left in FIG. When scanning is performed from left to right, by the sub-scanning motor 47,
In FIG. 7, a sample 8 for generating correction data is shown above.
0, that is, the sample stage 20 is intermittently moved by a distance corresponding to one scan line.

Then, in FIG. 7, the correction data generation sample 8 is moved by the main scanning motor 43 from left to right in FIG.
0, that is, when the sample stage 20 is moved and the surface of the correction data generation sample 80 is scanned from right to left by the laser light 4, the regular basis formed on the correction data generation sample 80 is obtained. According to the pattern, the color glass filter is excited, and the fluorescence 25 emitted from the surface of the color glass filter is photoelectrically detected by the photomultiplier 33, and is regularly formed on the correction data generation sample 80. Analog data of the basic pattern is generated.

The analog data generated by the photomultiplier 33 is converted into digital data by the A / D converter 34, and the digital data of the second line of the regular basic pattern is converted into the digital data.
5 is output.

Here, the jitter is measured by the main scanning motor 43.
When the sample stage 20 is reciprocated at high speed, the relative movement speed varies between the forward path and the return path due to the mechanical accuracy of the scanning mechanism, the load being moved, and the like. Are generated due to the deviation of the digital data of the first line of the regular basic pattern and the digital data of the second line of the regular basic pattern. Generating jitter correction data for correcting the jitter of the digital data on the second line of the regular basic pattern based on the digital data on the first line and the digital data on the second line of the basic pattern; By correcting the data of the even-numbered lines based on the generated jitter correction data, the jitter in the digital data is corrected. It is possible to reduce the coater.

Accordingly, the sample stage 20 is thus moved by the main scanning motor 43 in FIG.
The laser beam 4 reciprocates once in the main scanning direction indicated by the arrow X.
Thus, the correction data generation sample 80 is scanned by one round trip so that the digital data of the first line of the regular basic pattern and the second line of the regular basic pattern are scanned.
When the digital data of the line is generated, the control unit 75 turns off the first laser excitation light source 1 and outputs a jitter correction data generation signal to the data processing device 35.

FIG. 8 is a block diagram of the data processor 35 of the bidirectional scanning scanner according to the preferred embodiment of the present invention.

As shown in FIG. 8, the data processing device 3
Reference numeral 5 denotes a data storage unit 85 that stores data generated by the bidirectional scanning scanner, a correction data generation unit 86 that generates jitter correction data, and a storage unit that stores the jitter correction data generated by the correction data generation unit 86. A correction data storage unit 87 and a data processing unit 88 for performing data processing on data are provided.

When the sample scanning signal for generating correction data is input from the control unit 75 to the data processing device 35, the data processing device 35 is input from the A / D converter 34,
The digital data stored in the data storage unit 85 is output to the correction data generation unit 86.

FIG. 9 shows that the correction data generation sample 80 is scanned by the laser beam 4 for one round trip so that the color glass filter is excited and the fluorescence 25 emitted from the surface of the color glass filter is emitted. An A / D converter 34 which is photoelectrically detected by a photomultiplier 33
3 is a diagram in which digital data of a reference pattern which has been digitized by the above and input to the correction data generation unit 86 of the data processing device 35 is imaged.

In FIG. 9, the pulse-shaped curve indicated by A is obtained by scanning the reference pattern formed on the surface of the correction data generation sample 80 by the laser beam 4 from left to right in FIG. Is a generated reference pattern image, and a pulse-shaped curve indicated by B is obtained by scanning the reference pattern formed on the surface of the correction data generation sample 80 by the laser light 4 from right to left. ,
It is an image of the generated reference pattern.

As shown in FIG. 9, the position of the rising edge of each pulse of the pulse-shaped curve shown by B is calculated from the position of the rising edge of the corresponding pulse of the pulse-shaped curve shown by A by Δxi ( i is a positive integer indicating the number of pulses.), and jitter occurs.

Therefore, the correction data generating section 86 deviates the position of the rising portion of the corresponding pulse of the pulse-shaped curve shown by B from the position of the rising portion of each pulse of the pulse-shaped curve shown by A. The sum of the deviations Δxi is calculated, and the pulse-shaped curve indicated by B is shown in FIG. 9 so that the sum of the deviations Δxi of all the pulses is minimized.
Translated in the X direction, and the deviation amounts Δxi of all the pulses
Is determined as the jitter correction data used to correct the jitter of the digital data, and stored in the correction data storage unit 87.

In this embodiment, the main scanning motor 4
3 is a sample stage 20 at 200 mm / sec.
The control unit 75 is configured to be movable at a pixel pitch of 5 microns, 10 microns, 20 microns, 50 microns or 100 microns at a speed of 0 mm / sec or 800 mm / sec. Stage 20 is 200
It is moved in the main scanning direction at speeds of mm / sec, 400 mm / sec, and 800 mm / sec to generate jitter correction data, store it in the correction data storage unit 87, and store it in 10 μm, 20 μm, 50 μm or 100 μm. When the sample stage 20 is moved at a pixel pitch of microns, the data processing unit 88 moves the sample stage 20 at a pixel pitch of 5 microns at a corresponding main scanning speed in the main scanning direction to generate the sample stage 20. The jitter correction data stored in the correction data storage unit 87 is multiplied by a predetermined correction coefficient, and is used for data jitter correction.

[0170] The bidirectional scanning scanner according to the present embodiment configured as described above has a slide glass plate as a carrier and a large number of spots of a sample selectively labeled with a fluorescent dye as described below. The microarray formed on the slide glass plate is scanned by the laser light 4 to excite the fluorescent dye, and the fluorescence emitted from the fluorescent dye is photoelectrically detected to generate data for biochemical analysis. .

First, five microarrays using a slide glass plate as a sample 22 as a carrier are placed in the openings 51, 52, 53, 54, 55 of the sample carrier 21 in the direction indicated by the arrow A in FIG. Is inserted into.

The openings 51, 5 and 5 of the sample carrier 21
A leaf spring 51 is provided on one of the inner wall portions of 2, 53, 54, 55.
Since b, 52b, 53b, 54b, and 55b are attached, the sample 22 has respective openings 51,
Within 52,53,54,55, they are aligned along the other opposing inner wall.

At the same time, the openings 51, 52, 53, 5
The tops of the L-shaped leaf springs 51a, 52a, 53a, 54a, 55a abut against the sample 22 inserted in the insides 4, 55, and the leaf springs 51a, 52a, 53a, 54a,
With the spring force of 55a, the sample 22 respectively
The side regions on the side of the openings 51, 52, 53, 54, 55 are placed on the openings 51, 52, 53, 54, 55 along the longitudinal direction of the openings 51, 52, 53, 54, 55. The plate members 60, 61, 6 attached so as to protrude
It is urged against the surfaces of 2, 63, 64 and 65 and held on the sample carrier 21.

Here, plate members 60, 61, 62, 63, 64, 65 are provided on the surface of the frame body 50 with the openings 5.
The side regions on the 1, 52, 53, 54, 55 side are the openings 5
Along the longitudinal direction of 1, 52, 53, 54, 55, it protrudes above the openings 51, 52, 53, 54, 55,
Attached, the sample 22 includes leaf springs 51a, 52
a, 53a, 54a, and 55a are configured to be urged against the surfaces of the plate members 60, 61, 62, 63, 64, and 65, respectively, and held on the sample carrier 21 by the spring force of the sample carriers 21. The carrier 21 has a frame body 50 formed by processing one plate-like member.
Are placed on the sample stage 20 and set on the sample stage 20, so that the plate member 60 on which the sample 22 is supported on its surface,
The surfaces of the sample carriers 21, 62, 63, 64, 65 and the surface on which the sample carrier 21 is supported by the sample stage 20 are always in the same plane.
One and five samples 22 can be easily adjusted without complicated operations of adjusting the position of each sample 22 in the height direction.
Can always be set to the sample stage 20 with the same positional relationship.

Thus, when the sample carrier 21 holding the five microarrays, which are the samples 22 using the slide glass plate as the carrier, is set on the sample stage 20, the type of the sample carrier 21 is detected by the carrier sensor 70, The carrier detection signal is output to control unit 75.

Upon receiving the carrier detection signal from the carrier sensor 70, the control unit 75 outputs a drive signal to the switching member motor 72 based on the carrier detection signal, and causes the confocal switching member 31 to rotate the pinhole having the smallest diameter. 32a is moved so as to be located in the optical path.

Next, when the operator inputs the type of fluorescent substance as a labeling substance and the start signal to the keyboard 73, an instruction signal is output from the keyboard 73 to the control unit 75.

For example, the type of fluorescent substance is Cy-
When 5 (registered trademark) is input, the control unit 75 outputs a drive signal to the filter unit motor 71 in accordance with the input instruction signal, moves the filter unit 27, and cuts light having a wavelength of 640 nm. Then, a filter 28a having a property of transmitting light having a wavelength longer than 640 nm is located in the optical path, and a drive signal is output to the first laser excitation light source 1 to be turned on.

The laser light 4 emitted from the first laser excitation light source 1 is converted into parallel light by a collimator lens 5, then reflected by a mirror 6, and is reflected by a first dichroic mirror 7 and a second dichroic mirror. 8 and enter the optical head 15.

The laser beam 4 incident on the optical head 15 is
The sample 22 reflected by the mirror 16, passes through the hole 17 formed in the perforated mirror 18, is condensed by the lens 19, and is set on the sample stage 20.
Is incident on the microarray.

The sample stage 20 is moved by the main scanning motor 43 in the main scanning direction indicated by the arrow X in FIG.
The sample carrier 2 is moved at a high speed of 0 mm / sec and moved by the sub-scanning motor 47 in the sub-scanning direction indicated by the arrow Y in FIG.
The five samples 22 set to 1, that is, the entire surface of the five microarrays are sequentially scanned.

When the laser beam 4 is irradiated, the probe D
A fluorescent dye that labels NA, for example, Cy-5 is excited, and fluorescence 25 is emitted. When a slide glass plate is used as a carrier for the microarray,
Since the fluorescent dye is distributed only on the surface of the slide glass plate, the fluorescence 25 is also emitted only from the surface of the slide glass plate.

The fluorescent light 25 emitted from the surface of the slide glass plate is converted into parallel light by the lens 19, reflected by the perforated mirror 18, and filtered by the filter unit 27.
Incident on.

The filter unit 27 includes a filter 28a
Has been moved so as to be located in the optical path,
5 enters the filter 28a, cuts light having a wavelength of 640 nm, and transmits only light having a wavelength longer than 640 nm.

The fluorescence 25 transmitted through the filter 28a is reflected by the mirror 29 and is imaged by the lens 30.

Prior to the irradiation of the laser beam 4, the confocal switching member 31 is moved so that the pinhole 32a having the smallest diameter is located in the optical path, so that the fluorescent light 25 forms an image on the pinhole 32a. Then, the data is photoelectrically detected by the photomultiplier 33 and analog data is generated.

As described above, using the confocal optical system, the fluorescence 25 emitted from the fluorescent dye on the surface of the slide glass plate was used.
Is guided to the photomultiplier 33 and photoelectrically detected, so that noise in data can be minimized.

The analog data generated by the photo multiplier 33 is converted to digital data by the A / D converter 34 and sent to the data processing device 35.

Sample 2 sent to the data processor 35
The digital data of No. 2 is stored in the data storage unit 85 of the data processing device 35, and is output to the data processing unit 88 when the scanning of the five microarrays held by the sample carrier 21 with the laser light 4 is completed.

When the digital data of the sample 22 is input from the data storage unit 85, the data processing unit 88
From the correction data storage unit 87, the jitter correction data of the corresponding main scanning speed is read.

The data processing section 88 corrects the jitter correction data of the corresponding main scanning speed read from the correction data storage section 87, if necessary, according to the pixel pitch.
The digital data of the even-numbered line of the sample 22 is corrected based on the jitter correction data.

Correction data storage section 8 of data processing device 35
7 stores jitter correction data generated by moving the sample stage 20 in the main scanning direction at a pixel pitch of 5 microns at a speed of 200 mm / sec, 400 mm / sec, and 800 mm / sec, respectively. The sample stage 20 has a pixel pitch of 5 microns.
To 200 mm / sec, 400 mm / sec or 800 mm
When the digital data of the sampling 22 is generated by moving in the main scanning direction at a speed of / sec, the data processing unit 88 reads the corresponding jitter correction data from the correction data storage unit 87, and When the digital data for sampling 22 is generated by moving the sample stage 20 at a pixel pitch of 10, 20, 50, or 100 microns, the data processing is performed. The unit 88 moves the sample stage 20 at a pixel pitch of 5 microns at a corresponding main scanning speed.
By moving the jitter correction data in the main scanning direction, the generated jitter correction data is read from the correction data storage unit 87, multiplied by a predetermined correction coefficient, and corrected, thereby performing the jitter correction of the digital data of the sampling 22.

Here, the jitter correction data indicates that the position of the rising edge of each pulse of the pulse-shaped curve indicated by B corresponding to the digital data on the second line of the regular basic pattern is the regular basic pattern. From the position of the rising edge of the corresponding pulse of the pulse-shaped curve indicated by A corresponding to the digital data of the first line, the sum of the deviating deviation amounts Δxi is obtained, and the deviation amounts Δ of all the pulses are obtained.
The pulse-shaped curve indicated by B is translated in the X direction in FIG. 9 so that the sum of xi is minimized. Since the jitter correction data used to correct the data jitter is determined and generated, the digital data of the even-numbered line of the sample 22 is corrected based on the jitter correction data, thereby obtaining the sample 22. Jitter in the digital data of the digital camera can be minimized.

On the other hand, a fluorescent sample using a transfer support containing denatured DNA selectively labeled with a fluorescent dye as a carrier was scanned with a laser beam 4 to excite the fluorescent dye and release it from the fluorescent dye. Fluorescence is detected photoelectrically,
When generating data for biochemical analysis, instead of the sample carrier 21 shown in FIG. 5, a fluorescent sample 22 using a transfer support containing denatured DNA selectively labeled with a fluorescent dye as a carrier is used. Is set on the sample stage 20.

Thus, when the sample carrier 21 holding the fluorescent sample 22 is set on the sample stage 20, the type of the sample carrier 21 is detected by the carrier sensor 70, and a carrier detection signal is output to the control unit 75. You.

Upon receiving the carrier detection signal from the carrier sensor 70, the control unit 75 outputs a drive signal to the switching member motor 72 based on the carrier detection signal, and causes the confocal switching member 31 to rotate the pinhole having the largest diameter. 32c is moved so as to be located in the optical path.

Next, when the type of the fluorescent substance as the labeling substance and the start signal are input to the keyboard 73 by the operator, an instruction signal is output from the keyboard 73 to the control unit 75.

For example, when the sample is labeled with rhodamine, the control unit 75 selects the second laser excitation light source 2 because rhodamine can be most efficiently excited by a laser having a wavelength of 532 nm. At the same time, the filter 32b is selected, a drive signal is output to the filter unit motor 71, the filter unit 27 is moved, light having a wavelength of 532 nm is cut, and light having a wavelength longer than 532 nm is transmitted. The filter 28b is positioned in the optical path of the fluorescent light 25, and outputs a drive signal to the second laser excitation light source 2 to turn it on.

The light emitted from the second laser excitation light source 2
The laser beam 4 having a wavelength of 32 nm is converted into parallel light by the collimator lens 9 and then enters the first dichroic mirror 7 and is reflected.

The laser beam 4 reflected by the first dichroic mirror 7 is applied to the second dichroic mirror 8
And enters the optical head 15.

The laser beam 4 incident on the optical head 15 is
The light is reflected by the mirror 16, passes through the hole 17 formed in the perforated mirror 18, is condensed by the lens 19, and enters the fluorescent sample 22 set on the sample stage 20.

The sample stage 20 is moved by the main scanning motor 43 in the main scanning direction indicated by the arrow X in FIG.
The sample carrier 2 is moved at a high speed of 0 mm / sec and moved by the sub-scanning motor 47 in the sub-scanning direction indicated by the arrow Y in FIG.
The entire surface of the fluorescent sample 22 set to 1 is scanned.

When irradiated with the laser beam 4, a fluorescent dye, for example, rhodamine, which labels the sample, is excited,
Fluorescence 25 is emitted. When a transfer support is used as the carrier of the fluorescent sample 22, the fluorescent dye is distributed in the depth direction of the transfer support, so from a predetermined range in the depth direction of the transfer support, The fluorescent light 25 is emitted, and the position of the light emitting point in the depth direction also changes.

Fluorescent sample 22 using transfer support as carrier
The fluorescent light 25 emitted from is converted into parallel light by the lens 19, reflected by the perforated mirror 18, and enters the filter unit 27.

The filter unit 27 includes a filter 28b
Has been moved so as to be located in the optical path,
5 is incident on the filter 28b and cuts off light having a wavelength of 532 nm, and transmits only light having a wavelength longer than 532 nm.

The fluorescence transmitted through the filter 28b is reflected by the mirror 29 and collected by the lens 30, but the fluorescence 25 is emitted from a predetermined range in the depth direction of the transfer support. No image.

Prior to the irradiation of the laser beam 4, the confocal switching member 31 is moved so that the pinhole 32c having the largest diameter is located in the optical path. , And is photoelectrically detected by the photomultiplier 33 to generate analog data. Therefore, although a confocal optical system is used to detect the fluorescent light 25 emitted from the fluorescent dye on the surface of the microarray using the slide glass plate as a carrier at a high S / N ratio, the transfer support is performed. Fluorescence 25 emitted from a predetermined range in the depth direction of the body can also be detected with a high signal intensity.

The analog data generated by the photo multiplier 33 is converted to digital data by the A / D converter 34 and sent to the data processing device 35.

The digital data sent to the data processing unit 35 is stored in the data storage unit 85 of the data processing unit 35 and is generated by the laser beam 4 of the fluorescent sample 22 using the transfer support held by the sample carrier 21 as a carrier. When the scanning is completed, the data is output to the data processing unit 88.

When digital data of the sample 22 is input from the data storage unit 85, the data processing unit 88
From the correction data storage unit 87, the jitter correction data of the corresponding main scanning speed is read.

The data processing section 88 corrects the jitter correction data of the corresponding main scanning speed read from the correction data storage section 87, if necessary, according to the pixel pitch.
The digital data of the even-numbered line of the sample 22 is corrected based on the jitter correction data.

Here, the jitter is measured by the main scanning motor 43.
When the sample stage 20 is reciprocated at high speed, the relative movement speed varies between the forward path and the return path due to the mechanical accuracy of the scanning mechanism, the load being moved, and the like. The color glass filter of the correction data generation sample 80 is excited by the laser light 4 having a wavelength of 640 nm regardless of the wavelength of the laser light 4 because the color glass filter Detects the fluorescent light emitted from, and generates the jitter correction data generated and stored in the correction data storage unit 87 based on the digital data of the color glass filter forming the generated regular basic pattern. This can be used to correct the jitter of the digital data of the sample 22.

The jitter correction data is such that the rising position of each pulse of the pulse-shaped curve indicated by B corresponding to the digital data on the second line of the regular basic pattern is the regular basic pattern. From the position of the rising edge of the corresponding pulse of the pulse-shaped curve indicated by A corresponding to the digital data on the first line, the sum of the deviating deviation amounts Δxi is obtained, and the pulse-shaped curve indicated by B is obtained. In FIG. 9, the pulse-shaped curve indicated by B is changed so that the sum of the deviation amounts Δxi of all the pulses
Translated in the X direction, and the deviation amounts Δxi of all the pulses
Is determined as the jitter correction data used to correct the jitter in the digital data of the sample 22, and the generated parallel movement amount is minimized. By correcting the digital data on the even-numbered line, it is possible to minimize the jitter in the digital data of the sample 22.

On the other hand, a stimulable phosphor layer containing a stimulable phosphor is formed on a carrier such as a membrane filter on which a number of spots of a sample selectively labeled with a radioactive labeling substance are formed. The stimulable phosphor layer of the stimulable phosphor sheet on which the positional information of the radioactive labeling substance obtained by exposing the stimulable phosphor layer is brought into close contact with the stimulable phosphor sheet, FIG. 5 shows a case in which the stimulable phosphor is excited by scanning with the stimulable phosphor, and the stimulable phosphor emitted from the stimulable phosphor is photoelectrically detected to generate data for biochemical analysis. Instead of the sample carrier 21 shown, a sample carrier 21 holding a stimulable phosphor sheet on which a stimulable phosphor layer is formed is set on the sample stage 20.

When the sample carrier 21 holding the stimulable phosphor sheet on which the stimulable phosphor layer is formed is set on the sample stage 20, the type of the sample carrier 21 is detected by the carrier sensor 70, and the carrier is detected. The detection signal is output to the control unit 75.

Upon receiving the carrier detection signal from the carrier sensor 70, the control unit 75 outputs a drive signal to the switching member motor 72 based on the carrier detection signal, and causes the confocal switching member 31 to move the pin having an intermediate diameter. The hole 32b is moved so as to be located in the optical path.

Next, the control unit 75 outputs a drive signal to the filter unit motor 71 in accordance with the input instruction signal, moves the filter unit 27, and controls the stimulable phosphor emitted from the stimulable phosphor. A filter 28d having a property of transmitting only light in the wavelength range and cutting light having a wavelength of 640 nm is located in the optical path, and outputs a drive signal to the first laser excitation light source 1;
Turn on.

The laser light 4 emitted from the first laser excitation light source 1 is converted into parallel light by a collimator lens 5, then reflected by a mirror 6, and is reflected by a first dichroic mirror 7 and a second dichroic mirror. 8 and enter the optical head 15.

The laser beam 4 incident on the optical head 15 is
The sample 22 reflected by the mirror 16, passes through the hole 17 formed in the perforated mirror 18, is condensed by the lens 19, and is set on the sample stage 20.
Into the stimulable phosphor sheet.

The sample stage 20 is moved by the main scanning motor 43 in the main scanning direction indicated by the arrow X in FIG.
The sample carrier 2 is moved at a high speed of 0 mm / sec and moved by the sub-scanning motor 47 in the sub-scanning direction indicated by the arrow Y in FIG.
The entire surface of the stimulable phosphor layer of the stimulable phosphor sheet set to 1 is scanned.

Upon irradiation with the laser light 4, the stimulable phosphor contained in the stimulable phosphor layer is excited, and the stimulable phosphor 2 is irradiated.
5 is released. In the case of the stimulable phosphor sheet, the stimulable phosphor is contained in the stimulable phosphor layer and is distributed to some extent in the depth direction of the stimulable phosphor layer. The photostimulable light is emitted from a predetermined range in the depth direction of the luminescent phosphor layer, and the position of the light emitting point in the depth direction also changes. However, since the stimulable phosphor layer is thin, the light emitting points are not distributed in the depth direction as in the case of the transfer support.

Stimulation 25 released from the stimulable phosphor layer
Is converted into parallel light by the lens 19, reflected by the perforated mirror 18, and enters the filter unit 27.

The filter unit 27 includes a filter 28d
Is moved so as to be located in the optical path, the stimulating light 25 enters the filter 28d, the light having the wavelength of 640 nm is cut, and the stimulating light 25 in the wavelength region of the stimulating light emitted from the stimulable phosphor is emitted. Only light is transmitted.

The stimulating luminescence 25 transmitted through the filter 28d is
The light is reflected by the mirror 29 and collected by the lens 30, but the stimulable light is emitted from a predetermined range in the depth direction of the stimulable phosphor layer formed on the stimulable phosphor sheet. No imaging.

Prior to the irradiation of the laser beam 4, the confocal switching member 31 is moved so that the pinhole 32b having an intermediate diameter is located in the optical path, so that the photostimulable light has an intermediate diameter. After passing through the pinhole 32b, it is photoelectrically detected by the photomultiplier 33 and analog data is generated. Therefore, although the confocal optical system is used to detect the fluorescence 25 emitted from the fluorescent dye on the surface of the microarray using the slide glass plate as the carrier at a high S / N ratio, the accumulation property is high. The photostimulable light 25 emitted from a predetermined range in the depth direction of the photostimulable phosphor layer formed on the phosphor sheet can be detected with a high signal intensity.

The analog data generated by the photo multiplier 33 is converted into digital data by the A / D converter 34 and sent to the data processing device 35.

The digital data sent to the data processing unit 35 is stored in the data storage unit 85 of the data processing unit 35, and the laser light of the stimulable phosphor layer of the stimulable phosphor sheet held by the sample carrier 21 is stored. 4 is completed, the data is output to the data processing unit 88.

When the digital data of the sample 22 is input from the data storage unit 85, the data processing unit 88
From the correction data storage unit 87, the jitter correction data of the corresponding main scanning speed is read.

Here, the jitter is measured by the main scanning motor 43.
When the sample stage 20 is reciprocated at high speed, the relative movement speed varies between the forward path and the return path due to the mechanical accuracy of the scanning mechanism, the load being moved, and the like. Is generated due to the displacement of the photostimulable phosphor, so that the stimulable phosphor is excited, the stimulable phosphor 25 emitted from the stimulable phosphor is detected, and digital data of the sample 22 is generated. Also in this case, the color glass filter of the correction data generation sample 80 is excited by the laser light 4 having a wavelength of 640 nm, and the fluorescence emitted from the color glass filter is detected.
Generated based on the digital data of the color glass filter forming the generated regular basic pattern,
The jitter of the digital data of the sample 22 can be corrected using the jitter correction data stored in the correction data storage unit 87.

Here, the jitter correction data is such that the position of the leading edge of each pulse of the pulse-shaped curve indicated by B corresponding to the digital data on the second line of the regular basic pattern is the regular basic pattern. From the position of the rising edge of the corresponding pulse of the pulse-shaped curve indicated by A corresponding to the digital data of the first line, the sum of the deviated deviation amounts Δxi is obtained, and the pulse-shaped curve indicated by B is obtained. In FIG. 9, the pulse-shaped curve indicated by B is changed so that the sum of the deviation amounts Δxi of all the pulses
Translated in the X direction, and the deviation amounts Δxi of all the pulses
Is determined as the jitter correction data used to correct the jitter of the digital data of the sample 22, and is generated. Therefore, based on the jitter correction data, By correcting the digital data on the even-numbered line, it is possible to minimize the jitter in the digital data of the sample 22.

In this embodiment, the chromium deposition film 81
The correction data generation sample 80 in which the regular basic pattern of the color glass filter is formed in the portion where the chromium vapor deposition film 81 is not formed is formed by the main scanning motor 43. In the main scanning direction, the laser beam 4 is moved by the sub-scanning motor 47 in the sub-scanning direction. The data generation sample 80 is scanned to excite a color glass filter that forms a regular basic pattern, and the fluorescence 25 emitted from the color glass filter is photoelectrically detected, digitized, and formed by the color glass filter. To generate digital data of a regular basic pattern.

Further, in this embodiment, a color glass filter forming a regular basic pattern is scanned by a laser beam 4 from right to left in FIG. The position of the rising portion of the pulse-shaped curve indicated by B corresponding to the digital data of the second line of the regular basic pattern obtained by digitization is determined by the color glass filter that forms the regular basic pattern. , From left to right, the first line of the regular basic pattern and the second line of the basic pattern obtained by detecting and digitizing the fluorescent light 25 scanned, excited and emitted by the laser light 4 Based on the digital data of the first line, each pulse of the pulse-shaped curve indicated by B corresponding to the digital data of the second line of the regular basic pattern. The position of the rising edge of the pulse is shifted from the position of the rising edge of the corresponding pulse of the pulse-shaped curve indicated by A corresponding to the digital data of the first line of the regular basic pattern. The pulse-shaped curve indicated by B is translated in the X direction in FIG. 9 so that the sum of the deviation amounts Δxi of all the pulses of the pulse-shaped curve indicated by B is minimized. , The amount of translation that minimizes the sum of the deviation amounts Δxi is determined as jitter correction data used to correct data jitter.

In this embodiment, the microarray using a slide glass plate as a carrier and the fluorescent sample 22 using a transfer support as a carrier are irradiated with laser light 4 to excite the fluorescent dye and emit the fluorescent dye. The laser light 4 is applied to the digital data of the sample 22 and the stimulable phosphor layer formed on the stimulable phosphor sheet by irradiating the stimulable fluorescent light with the digital data of the sample 22 obtained by photoelectrically detecting the generated fluorescence. Exciting the body and stimulating light emitted from the stimulable phosphor 2
Sample 2 obtained by photoelectrically detecting and digitizing 5
The even-numbered line of the second digital data is corrected by the jitter correction data generated as described above.

Therefore, according to the present embodiment, when the sample stage 20 is reciprocated at a high speed by the main scanning motor 43, the sample stage 20 may have a forward path and a backward path depending on the mechanical accuracy of the scanning mechanism and the load to be moved. In addition, it is possible to minimize the jitter generated in the digital data of the sample 22 due to the variation in the relative moving speed and the shift in the data sampling timing.

Further, according to the present embodiment, a microarray using a slide glass plate as a carrier is irradiated with a laser beam to excite a fluorescent dye, detect fluorescence emitted from the fluorescent dye, and When generating data, the fluorescent sample 22 using the transfer support as a carrier is irradiated with laser light to excite the fluorescent dye, detect the fluorescence emitted from the fluorescent dye, and generate digital data of the sample 22. The stimulable phosphor layer formed on the case and the stimulable phosphor sheet is irradiated with a laser beam 4 to excite the stimulable phosphor and to stimulate the stimulable phosphor 25 emitted from the stimulable phosphor.
Is detected, and the digital data of the sample 22 is generated in any case, the same jitter correction data is used to correct the jitter in the digital data of the sample 22. The laser beam 4 of the wavelength is used to excite the color glass filter of the correction data generation sample 80, to detect the fluorescence emitted from the color glass filter, and to generate digital data of a regular basic pattern of the generated color glass filter. , The jitter in the digital data of the sample 22 can be minimized simply by generating the jitter correction data and storing it in the correction data storage unit 87. The main scanning motor 43 prevents the sample stage 2 from being unnecessarily increased in size. When There is reciprocated in forward and the backward path, due to how it was moved to be different, the sample 2
2 can be minimized.

Further, according to the present embodiment, the correction data storage unit 87 stores the sample stages 20 at a pixel pitch of 5 μm at 200 mm / sec.
The sample stage 2 is moved in the main scanning direction at a speed of m / sec and 800 mm / sec, and the generated jitter correction data is stored.
0 is moved in the main scanning direction, and when digital data of the sampling 22 is generated, the data processing unit 88
Reads the corresponding jitter correction data from the correction data storage unit 87 and executes the jitter correction of the digital data of the sampling 22,
When the digital data of the sampling 22 is generated by moving the sample stage 20 at a pixel pitch of 0 micron, 50 micron, or 100 micron,
The data processing unit 88 reads the generated jitter correction data from the correction data storage unit 87 by moving the sample stage 20 in the main scanning direction at a corresponding main scanning speed at a pixel pitch of 5 microns, and Is multiplied by the correction coefficient, and the jitter is corrected for the digital data of the sampling 22. Therefore, the sampling data can be efficiently sampled without using a large-capacity memory as the correction data storage unit 87. It is possible to greatly reduce jitter in the 22 digital data.

FIG. 10 is a schematic front view of a member for generating jitter correction data used for generating jitter correction data in the bidirectional scanning scanner according to another preferred embodiment of the present invention.

As shown in FIG. 10, the member 80 for generating jitter correction data according to the present embodiment has substantially the same size as one microarray, and a regular basic pattern is formed over the entire surface thereof. Is formed.

Also in this embodiment, the correction data generation sample 80 is formed of a color glass filter, and the surface of the color glass filter has a chromium vapor deposited film 81 formed thereon.
Are formed, and a regular basic pattern of a color glass filter is formed in a portion where the chromium deposition film 81 is not formed.

In generating the jitter correction data,
First, the jitter correction data generating member 80 is moved in the direction indicated by the arrow A in FIG.
And is aligned along the other opposing inner wall by the leaf spring 51b.

At the same time, the top of the L-shaped leaf spring 51a abuts against the jitter correction data generating member 80 inserted in the opening 51, and the spring force of the leaf spring 51a causes the top of the opening 51 to move. Is urged against the surface of the attached plate member 60 so as to protrude over the opening 51 along the longitudinal direction of the opening 51 and is held by the sample carrier 21.

When the member 80 for generating jitter correction data is set in this way, the sample carrier 21 is set on the sample stage 20.

Next, when the operator inputs a correction data generation sample scanning signal to the keyboard 73, the correction data generation sample scanning signal is output to the control unit 75.

Upon receiving the correction data generation sample scanning signal, the control unit 75 outputs a drive signal to the filter unit motor 71 so that the filter unit 2 can be positioned in the optical path of the fluorescent light 25.
7 and a drive signal is output to the switching member motor 72 to move the confocal switching member 31 so that the pinhole 32a is positioned in the optical path of the fluorescence 25.

The correction data generation sample scanning signal is sent from the control unit 75 to the data processing device 3 at the same time.
5 is output.

Next, the control unit 75 activates the first laser excitation light source 1 and emits the laser light 4 of 640 nm.

The laser light 4 emitted from the first laser excitation light source 1 is converted into parallel light by a collimator lens 5, then reflected by a mirror 6, and is reflected by a first dichroic mirror 7 and a second dichroic mirror. 8 and enter the optical head 15.

The laser beam 4 incident on the optical head 15 is
The light is reflected by the mirror 16, passes through the hole 17 formed in the perforated mirror 18, is condensed by the lens 19, and enters the correction data generation sample 80 set on the sample stage 20.

Also in this embodiment, the correction data generation sample 80 is formed of a color glass filter, and the surface of the color glass filter has a chromium vapor deposited film 81 formed thereon.
Is formed, and a regular basic pattern of a color glass filter is formed in a portion where the chromium vapor-deposited film 81 is not formed. Therefore, the chromium vapor-deposited film 81 is not formed, and a regular basic pattern is formed. The surface of the color glass filter is excited by the laser light 4 and the fluorescence 25 is emitted.

The fluorescent light emitted from the surface of the color glass filter forming the regular basic pattern is
Is converted into parallel light, is reflected by the perforated mirror 18, and enters the filter unit 27.

The filter unit 27 includes a filter 28a
Is moved so as to be located in the optical path, the fluorescent light enters the filter 28a, the light having a wavelength of 640 nm is cut, and only the light having a wavelength longer than 640 nm is transmitted.

The fluorescent light transmitted through the filter 28a is reflected by the mirror 29 and is imaged by the lens 30.

Prior to the irradiation of the laser beam 4, the confocal switching member 31 is moved so that the pinhole 32a having the smallest diameter is located in the optical path, so that the fluorescent light is focused on the pinhole 32a. , Photomultiplier 33
Is detected photoelectrically, and analog data is generated.

The analog data generated by the photomultiplier 33 is converted into digital data by the A / D converter 34 and sent to the data processor 35.

The sample stage 20 is reciprocated at high speed in the main scanning direction indicated by an arrow X in FIG. , And is moved in the main scanning direction indicated by the arrow Y, and the correction data generation sample 80, that is, from right to left in FIG.
The sample stage 20 is moved, and the surface of the correction data generation sample 80 is scanned by the laser light 4 from left to right.

As a result, the correction data generation sample 80
According to the regular basic pattern formed in the above, the color glass filter is scanned from left to right by the laser beam 4, the color glass filter is excited, and the fluorescence 25 emitted from the surface of the color glass filter is emitted. The analog data of the regular basic pattern detected photoelectrically by the photomultiplier 33 and formed on the correction data generation sample 80 is generated.

The analog data generated by the photomultiplier 33 is converted to digital data by the A / D converter 34, and the digital data of the first line of the regular basic pattern is converted to digital data by the data processing device 3.
5 is output.

In FIG. 10, the correction data generation sample 80, that is, the sample stage 20, is moved by the main scanning motor 43 from right to left in FIG. When scanning is performed from left to right, by the sub-scanning motor 47,
In FIG. 10, a sample 8 for generating correction data is shown above.
0, that is, the sample stage 20 is intermittently moved by a distance corresponding to one scan line.

Next, the correction data generation sample 80, that is, the sample stage 20, is moved from left to right in FIG. 10 by the main scanning motor 43, and the surface of the correction data generation sample 80 is When scanning from right to left, the color glass filter is excited according to the regular basic pattern formed on the correction data generation sample 80, and the fluorescence 25 emitted from the surface of the color glass filter is emitted. The analog data of the regular basic pattern detected photoelectrically by the photomultiplier 33 and formed on the correction data generation sample 80 is generated.

The analog data generated by the photomultiplier 33 is converted to digital data by the A / D converter 34, and the digital data of the second line of the regular basic pattern is converted to the digital data by the data processor 3.
5 is output.

The same operation is repeated until the entire surface of the correction data generation sample 80 is scanned by the laser beam 4 and digital data of each line is generated.

The digital data of the regular basic pattern formed on the correction data generation sample 80 is sequentially
The data is input to the data storage unit 85 of the data generation device 35 and stored.

Thus, when the entire surface of the correction data generation sample 80 is scanned by the laser light 4 to generate digital data of each line, the control unit 75 turns off the first laser excitation light source 1. , And outputs a jitter correction data generation signal to the data processing device 35.

FIG. 11 shows that the color glass filter is excited by scanning the correction data generation sample 80 with the laser light 4, and the fluorescence 25 emitted from the surface of the color glass filter is photoelectrically converted by the photomultiplier 33. The digital data of the (2N-1) -th line of the reference pattern and the 2N-th digit of the reference pattern input to the correction data generating unit 86 of the data processing device 35 are digitized by the A / D converter 34 and input to the correction data generating unit 86 It is the drawing which imaged digital data of the line. Here, N is an integer of 1 or more.

Upon receiving the jitter correction data generation signal,
The correction data generation unit 86 performs the
The position of the rising edge of each pulse of the pulse-shaped curve corresponding to the digital data of the 2N-th line indicated by D is the pulse-shaped curve corresponding to the digital data of the (2N-1) -th line indicated by C. From the position of the rising edge of the corresponding pulse, the sum of the deviating deviation amounts Δxi is obtained, and the sum of the deviation amounts Δxi of all the pulses in the pulse-like curve indicated by D is minimized. 2
In FIG. 11, the pulse-shaped curve corresponding to the digital data of the N-th line is translated in the X direction, and the translation amount that minimizes the sum of the deviation amounts Δxi of all the pulses is represented by (2N−1). As the jitter correction data used to correct the jitter of the digital data of the 2Nth line with respect to the digital data of the 2nd line,
It is determined and stored in the correction data storage unit 87.

In this embodiment, the control unit 75 controls the sample stage 20 at 200 mm / sec, 400 mm / sec, and 800 mm / sec with a pixel pitch of 5 μm, in exactly the same manner as in the above embodiment.
At the speed of 1 / sec in the main scanning direction to generate jitter correction data and store it in the correction data storage unit 87;
10 microns, 20 microns, 50 microns or 1
When the sample stage 20 is moved at a pixel pitch of 00 microns, the data processing unit 88 moves the sample stage 20 at a pixel pitch of 5 microns at a corresponding main scanning speed in the main scanning direction to generate the data. The jitter correction data stored in the correction data storage unit 87 is multiplied by a predetermined correction coefficient, and is used for data jitter correction.

The bidirectional scanning scanner according to the present embodiment configured as described above uses a slide glass plate as a carrier and a large number of spots of a sample selectively labeled with a fluorescent dye as described below. The microarray formed on the slide glass plate is scanned by the laser light 4 to excite the fluorescent dye, and the fluorescence emitted from the fluorescent dye is photoelectrically detected to generate data for biochemical analysis. .

First, a sample carrier 21 holding five microarrays as a sample 22 using a slide glass plate as a carrier is set on a sample stage 20.

When the sample carrier 21 is set on the sample stage 20, the carrier sensor 70
The type of the sample carrier 21 is detected, and a carrier detection signal is output to the control unit 75.

Upon receiving the carrier detection signal from the carrier sensor 70, the control unit 75 outputs a drive signal to the switching member motor 72 based on the carrier detection signal, and causes the confocal switching member 31 to move the pinhole having the smallest diameter. 32a is moved so as to be located in the optical path.

Next, when the operator inputs the type of fluorescent substance as a labeling substance and the start signal to the keyboard 73, an instruction signal is output from the keyboard 73 to the control unit 75.

For example, the type of fluorescent substance is Cy-
When 5 (registered trademark) is input, the control unit 75 outputs a drive signal to the filter unit motor 71 in accordance with the input instruction signal, moves the filter unit 27, and cuts light having a wavelength of 640 nm. Then, a filter 28a having a property of transmitting light having a wavelength longer than 640 nm is located in the optical path, and a drive signal is output to the first laser excitation light source 1 to be turned on.

The laser light 4 emitted from the first laser excitation light source 1 is converted into parallel light by a collimator lens 5, then reflected by a mirror 6, and is reflected by a first dichroic mirror 7 and a second dichroic mirror. 8 and enter the optical head 15.

The laser beam 4 incident on the optical head 15 is
The sample 22 reflected by the mirror 16, passes through the hole 17 formed in the perforated mirror 18, is condensed by the lens 19, and is set on the sample stage 20.
Is incident on the microarray.

The sample stage 20 is moved by the main scanning motor 43 in the main scanning direction indicated by the arrow X in FIG.
The sample carrier 2 is moved at a high speed of 0 mm / sec and moved by the sub-scanning motor 47 in the sub-scanning direction indicated by the arrow Y in FIG.
The five samples 22 set to 1, that is, the entire surface of the five microarrays are sequentially scanned.

When the laser beam 4 is irradiated, the probe D
A fluorescent dye that labels NA, for example, Cy-5 is excited, and fluorescence 25 is emitted. When a slide glass plate is used as a carrier for the microarray,
Since the fluorescent dye is distributed only on the surface of the slide glass plate, the fluorescence 25 is also emitted only from the surface of the slide glass plate.

The fluorescent light 25 emitted from the surface of the slide glass plate is converted into parallel light by the lens 19, reflected by the perforated mirror 18, and filtered by the filter unit 27.
Incident on.

The filter unit 27 includes a filter 28a
Has been moved so as to be located in the optical path,
5 enters the filter 28a, cuts light having a wavelength of 640 nm, and transmits only light having a wavelength longer than 640 nm.

[0279] The fluorescence 25 transmitted through the filter 28a is reflected by the mirror 29 and is imaged by the lens 30.

Prior to the irradiation of the laser beam 4, the confocal switching member 31 is moved so that the pinhole 32a having the smallest diameter is located in the optical path, so that the fluorescent light 25 forms an image on the pinhole 32a. Then, the data is photoelectrically detected by the photomultiplier 33 and analog data is generated.

The analog data generated by the photo multiplier 33 is converted to digital data by the A / D converter 34 and sent to the data processing device 35.

Sample 2 sent to data processing unit 35
The digital data of No. 2 is stored in the data storage unit 85 of the data processing device 35, and is output to the data processing unit 88 when the scanning of the five microarrays held by the sample carrier 21 with the laser light 4 is completed.

When digital data of the sample 22 is input from the data storage unit 85, the data processing unit 88
From the correction data storage unit 87, the jitter correction data of the corresponding main scanning speed is read.

The data processing section 88 corrects the jitter correction data of the corresponding main scanning speed read from the correction data storage section 87, if necessary, according to the pixel pitch.
Based on the jitter correction data, (2N
-1) 2N for the digital data of the line
The digital data of the second line is sequentially corrected.

A fluorescent sample using a transfer support containing denatured DNA selectively labeled with a fluorescent dye as a carrier is irradiated with a laser beam 4 to excite the fluorescent dye and to emit fluorescence emitted from the fluorescent dye. Digital data obtained by photoelectrically detecting and a carrier such as a membrane filter on which a number of spots of a sample selectively labeled with a radioactive labeling substance are formed, a stimulable phosphor layer containing a stimulable phosphor. The stimulable phosphor sheet on which the positional information of the radioactive labeling substance obtained by exposing the stimulable phosphor layer is brought into close contact with the stimulable phosphor sheet on which the The jitter of the digital data obtained by exciting the stimulable phosphor and photoelectrically detecting the stimulable light emitted from the stimulable phosphor is also calculated using the jitter correction data of the 2Nth line. Embodiment Similarly, it is corrected.

According to this embodiment, the position of the rising portion of each pulse of the pulse-shaped curve indicated by D corresponding to the digital data of the 2N-th line of the regular basic pattern is determined by the jitter correction data. From the position of the leading edge of the corresponding pulse of the pulse-shaped curve C corresponding to the digital data of the (2N-1) -th line of the basic pattern, 2N indicated by D so that the sum of the deviation amounts Δxi of all the pulses of the pulse-shaped curve indicated by D is minimized.
In FIG. 11, the pulse-shaped curve corresponding to the digital data of the second line is translated in the X direction, and the translation amount at which the sum of the deviation amounts Δxi of all the pulses is minimized is represented by the digital value of the 2Nth line. Since the data is determined and generated as the jitter correction data used to correct the data jitter, the digital data of the 2Nth line of the sample 22 is corrected based on the jitter correction data, thereby It is possible to minimize the jitter in the digital data of No. 22.

Further, according to the present embodiment, the entire surface of the jitter correction data generating member 80 having substantially the same size as the microarray and having a regular basic pattern formed on the entire surface thereof is irradiated with the laser light. 4 and based on the digital data obtained by detecting the fluorescence, based on the digital data obtained by detecting the fluorescence, each pulse at the rising portion of the pulse-shaped curve indicated by D corresponding to the digital data of the 2Nth line of the regular basic pattern The position of the deviation Δxi that deviates from the position of the rising edge of the corresponding pulse of the pulse-shaped curve indicated by C corresponding to the digital data of the (2N−1) th line of the regular basic pattern. The sum is obtained, and the sum of the deviation amounts Δxi of all the pulses of the pulse-shaped curve indicated by D is minimized so that 2
In FIG. 11, the pulse-shaped curve corresponding to the digital data of the Nth line is translated in the X direction, and the translation amount at which the sum of the deviation amounts Δxi of all the pulses is minimized is represented by the 2Nth line. Since it is determined as the jitter correction data used to correct the jitter of the digital data, and the digital data of the 2Nth line of the sample 22 is corrected, even when the generated jitter differs depending on the scanning area, Jitter in the digital data of the sample 22 can be minimized.

FIG. 12 is a schematic perspective view of a sample carrier 21 set on a sample stage of a bidirectional scanning scanner according to another preferred embodiment of the present invention.

As shown in FIG. 12, in this embodiment, the sample carrier 21 is provided on the surface of one side portion 50a of the frame body 50 with a regular basic light and shade pattern 9.
0 is formed, and the surface of one side portion 50a of the frame body 50 constituting the sample carrier 21 constitutes a sample 80 for generating jitter correction data. The regular shading pattern is formed in the same regular basic pattern as that shown in FIG. 7.
It is formed in a portion that is not in contact with zero.

In the bidirectional scanning scanner, the laser beam 4 can be irradiated by the sub-scanning motor 47 onto the surface of one side portion 50a of the frame body 50 on which a regular basic pattern is formed. Is configured.

FIG. 13 is a block diagram of a data processing device of the bidirectional scanning scanner according to the present embodiment.

As shown in FIG. 13, the data processing device of the bidirectional scanning scanner according to the present embodiment further comprises:
Similarly to the above-described embodiment, a reference correction data storage unit 95 that stores the jitter correction data generated by the correction data generation unit 86 using the jitter correction data generation sample 80 is provided.

The bidirectional scanning scanner according to the present embodiment configured as described above generates jitter correction data as necessary and stores it in the correction data storage section 87 as follows. Using a slide glass plate as a carrier, a large number of spots of a sample selectively labeled with a fluorescent dye are scanned by a laser beam 4 on a microarray formed on the slide glass plate to excite the fluorescent dye, Fluorescence emitted from the dye is photoelectrically detected to generate data for biochemical analysis.

First, a sample carrier 21 holding five microarrays using a slide glass plate as a carrier is set on the sample stage 20 in the same manner as in the above embodiment.

Next, a user inputs a correction data generation sample scanning signal to the keyboard 73 as necessary.

In this embodiment, similarly to the above embodiment, the jitter correction data is generated in advance by the correction data generation unit 86 using the jitter correction data generation sample 80, and the reference correction data storage unit 95 is used.
Therefore, if necessary, new jitter correction data is generated. If no new jitter correction data is generated, the jitter correction data is stored in the reference correction data storage unit 95 based on the jitter correction data. And sample 2
The second digital data is configured to be corrected.

Therefore, the user operates the keyboard 73
Then, the jitter correction data is generated only when the correction data generation sample scanning signal is input.

The input correction data generation sample scanning signal is output to the control unit 75. Upon receiving the correction data generation sample scanning signal, the control unit 75 outputs a driving signal to the filter unit motor 71, The filter unit 27 is retracted from the optical path of the fluorescence or stimulating light 25, and a driving signal is output to the switching member motor 72 so that the confocal switching member 31 is moved so that the pinhole 32a is positioned in the optical path of the fluorescence 25. Move.

At the same time, the control unit 75 sends the correction data generation sample scanning signal to the data processing device 3.
5 is output.

Next, the control unit 75 outputs a drive signal to the sub-scanning motor 47, so that one side 50a of the frame body 50 constituting the sample carrier 21 is provided.
The regular basic shading pattern 90 formed on the surface of
The sample carrier 21 is irradiated so that the laser beam 4 can be irradiated.
Is moved, and then the first laser excitation light source 1 is started to operate at 640 nm.
Is emitted.

The laser light 4 emitted from the first laser excitation light source 1 is converted into parallel light by a collimator lens 5, and then reflected by a mirror 6, and is reflected by a first dichroic mirror 7 and a second dichroic mirror. 8 and enter the optical head 15.

The laser beam 4 incident on the optical head 15 is
One side of the frame body 50 that is reflected by the mirror 16, passes through the hole 17 formed in the perforated mirror 18, is condensed by the lens 19, and forms the sample carrier 21 set on the sample stage 20. 50a
Is incident on the regular basic shading pattern 90 formed on the surface of.

The laser beam 4 incident on the regular basic shading pattern 90 formed on the surface of the one side portion 50a of the frame body 50 constituting the sample carrier 21 is reflected by the surface of the sample carrier 21, The amount of reflected light is generated in accordance with the basic gray scale pattern 90.

The laser light 4 reflected by the surface of the sample carrier 21 on which the regular basic shading pattern 90 is formed is converted into parallel light by the lens 19 and reflected by the perforated mirror 18.

Here, since the filter unit 27 is held in a state of being retracted from the optical path of the fluorescence or stimulating light 25, the light is reflected by the surface of the sample carrier 21 on which the regular basic shading pattern 90 is formed. The laser light 4 is reflected by the mirror 29 and is imaged by the lens 30.

Prior to the irradiation of the laser beam 4, the confocal switching member 31 is moved so that the pinhole 32a having the smallest diameter is located in the optical path, so that a regular basic light and shade pattern 90 is formed. Sample carrier 21
The laser beam 4 reflected by the surface of the pinhole 3
An image is formed on 2a, and is photoelectrically detected by the photomultiplier 33 to generate analog data.

As described above, by using the confocal optical system, the laser beam 4 reflected by the surface of the sample carrier 21 on which the regular basic density pattern 90 is formed is guided to the photomultiplier 33 and photoelectrically. Since detection is performed, noise in data can be minimized.

The analog data generated by the photomultiplier 33 is converted into digital data by the A / D converter 34 and sent to the data processing device 35.

When the sample stage 20 is moved by one reciprocation in the main scanning direction by the main scanning motor 43 and is moved in the sub scanning direction by the sub scanning motor 47, the sample carrier 21 is formed. The regular basic shading pattern 90 formed on the surface of the one side portion 50 a of the frame body 50 is scanned by the laser light 4 for one reciprocation, and the regular basic shading pattern 90 formed on the surface of the sample carrier 21 is formed. The laser beam 4 reflected by the basic gray scale pattern 90 is photoelectrically detected by the photomultiplier 33, and analog data of a regular basic gray scale pattern is generated and digitized by the A / D converter 34. Digital data of a regular basic shading pattern is generated.

Therefore, when the regular basic gray pattern 90 formed on the surface of the sample carrier 21 is scanned by the laser beam 4 for one reciprocation, the first line of the regular basic gray pattern 90 And the digital data of the second line of the regular basic shading pattern 90 are generated and sent to the data processor 35.

When the digital data of the first line of the regular basic gradation pattern 90 and the digital data of the second line of the regular basic gradation pattern 90 are generated and sent to the data processing unit 35, the control unit The reference numeral 75 turns off the first laser excitation light source 1 and outputs a jitter correction data generation signal to the data processing device 35.

When the sample scanning signal for generating correction data is input from the control unit 75 to the data processor 35, the digital data input from the A / D converter 34 and stored in the data storage unit 85 is The correction data is output to the correction data generator 86.

The correction data generator 8 of the data processor 35
6 receives the jitter correction data generation signal from the control unit 75, and in the same manner as the embodiment shown in FIGS. 1 to 9, the rising of each pulse of the pulse-shaped curve indicated by A in FIG. From the position of the portion, the sum of the deviations Δxi at which the positions of the rising portions of the corresponding pulses of the pulse-shaped curve indicated by B are deviated is determined, and the sum of the deviations Δxi of all the pulses is minimized. , B are translated in the X direction in FIG. 9, and the translation amount that minimizes the sum of the deviation amounts Δxi of all the pulses is used to correct the jitter of the digital data. The data is determined as jitter correction data and stored in the correction data storage unit 87.

In this embodiment, the main scanning motor 4
3 is a sample stage 20 at 200 mm / sec.
The control unit 75 is configured to be movable at a pixel pitch of 5 microns, 10 microns, 20 microns, 50 microns or 100 microns at a speed of 0 mm / sec or 800 mm / sec. Stage 20 is 200
It is moved in the main scanning direction at speeds of mm / sec, 400 mm / sec, and 800 mm / sec to generate jitter correction data, store it in the correction data storage unit 87, and store it in 10 μm, 20 μm, 50 μm or 100 μm. When the sample stage 20 is moved at a pixel pitch of microns, the data processing unit 88 moves the sample stage 20 at a pixel pitch of 5 microns at a corresponding main scanning speed.
It is configured to move in the main scanning direction, multiply the jitter correction data generated and stored in the correction data storage unit 87 by a predetermined correction coefficient, and use the data for jitter correction of the data.

[0315] When the jitter correction data is stored in the correction data storage section 87 in this way, a start signal is input to the keyboard 73 by the user.

The start signal is sent to the control unit 7
5, when the control unit 75 receives the start signal, returns the sample stage 20 to the original position, detects the sample carrier and outputs an example signal to the carrier sensor 70, and outputs the type of the sample carrier 21. Is detected, and the control unit 75 outputs a carrier detection signal.

Next, when the type of the fluorescent substance as the labeling substance is input to the keyboard 73 by the user, an instruction signal is sent from the keyboard 73 to the control unit 75.
Is output to

When Cy-5 (registered trademark) is input as a type of fluorescent substance as a labeling substance, the control unit 75 outputs a drive signal to the filter unit motor 71 in accordance with the input instruction signal. Then, the filter unit 27 is moved to cut light having a wavelength of 640 nm, and a filter 28 a having a property of transmitting light having a wavelength longer than 640 nm is located in the optical path. A drive signal is output and turned on.

The laser light 4 emitted from the first laser excitation light source 1 is converted into parallel light by a collimator lens 5, then reflected by a mirror 6, and is reflected by a first dichroic mirror 7 and a second dichroic mirror. 8 and enter the optical head 15.

Thus, in the same manner as in the above embodiment, the five microarrays held on the sample carrier 21 set on the sample stage 20 are sequentially scanned by the laser light 4 to excite the fluorescent dye, The fluorescence emitted from the fluorescent dye is detected photoelectrically,
Digital data for biochemical analysis is generated.

In this embodiment, prior to generation of digital data of the sample 22, the user inputs a correction data generation sample scanning signal to the keyboard 73, and the one side surface 50a of the frame body 50 of the sample carrier 21. When the jitter correction data is generated in accordance with the regular basic shading pattern 90 formed in
The data processing unit 88 reduces the jitter in the digital data of the sample 22 based on the new jitter correction data stored in the correction data storage unit 87 in exactly the same manner as the embodiment shown in FIGS. Will be corrected.

On the other hand, the user does not input the correction data generating sample scanning signal to the keyboard 73, and is formed on one side surface 50a of the frame body 50 of the sample carrier 21 prior to generating the digital data of the sample 22. When no new jitter correction data is generated according to the regular basic shading pattern 90, the data processing unit 88 uses the correction data generation sample 80 to perform correction data generation in the same manner as in the above embodiment. Based on the jitter correction data generated by the generation unit 86 and stored in the reference correction data storage unit 95, the jitter in the digital data of the sample 22 is exactly the same as in the embodiment shown in FIGS. Is corrected.

According to the present embodiment, before the digital data of the sample 22 is generated, the jitter in the digital data of the sample 22 can be corrected by generating the jitter correction data as necessary. Even when the elongation of the timing belt 45 changes with time and the generated jitter becomes different, the jitter in the digital data of the sample 22 can be minimized. The present invention is not limited to the above embodiments, and various modifications can be made within the scope of the invention described in the claims, which are also included in the scope of the present invention. Needless to say.

For example, in the embodiment shown in FIG. 12 and FIG.
It is configured to generate the jitter correction data by inputting the correction data generation sample scanning signal and the jitter correction data generation signal. However, when the start signal is input, the jitter correction data is automatically generated. Is generated, and then a digital of the sample 22 is generated.

Further, in the embodiment shown in FIGS. 12 and 13, a regular basic shading pattern 90 is formed on a part of one side face 50a of the frame body 50 constituting the sample carrier 21. However, when the sample 22 held on the sample carrier 21 is irradiated with the laser beam 4, if there is no problem, the regular
0 can be formed at any position of the sample carrier 21, and it is not always necessary to form a regular basic pattern 90 on a part of one side surface 50 a of the frame body 50 constituting the sample carrier 21. And, for example, the sample stage 2 on the optical head 15 side.
Outside the portion where the 0 sample carrier 21 is placed,
For example, a regular basic gray pattern 90 may be formed in the bidirectional scanning scanner itself, such as forming a regular basic gray pattern 90, and jitter correction data may be generated as necessary.

In the above embodiment, the sample stage 20 was moved at a pixel pitch of 5 μm to 200 mm / sec, 400 mm / sec and 800 mm / sec, respectively.
When the sample stage 20 is moved at a pixel pitch of 10 μm, 20 μm, 50 μm or 100 μm by moving the sample stage 20 at a pixel pitch of 10 μm, 20 μm, 50 μm or 100 μm, The jitter correction data of the scanning speed is multiplied by a correction coefficient, corrected, and the jitter correction of the digital data of the sample is performed using the corrected jitter correction data.
For each main scanning speed and each pixel pitch, jitter correction data is generated and stored in the correction data storage unit 87 or the reference correction data storage unit 95, and the corresponding jitter correction data is stored in accordance with the main scanning speed and the pixel pitch. It can also be configured to read out and perform digital jitter correction of the sample.

Further, in the above embodiment, the sample stage 20 was moved at 200 mm / sec, 400 mm / sec and 800 mm / pixel at a pixel pitch of 5 μm, respectively.
The jitter correction data is generated by moving the sample stage 20 in the main scanning direction at a speed of 200 mm / sec at a pixel pitch of 5 microns. Then, jitter correction data is generated and stored in the correction data storage unit 87 or the reference correction data storage unit 95, and the jitter correction data is stored at 400 mm / sec or 8 mm.
When the sample stage 20 is moved at a speed of 00 mm / sec, the jitter is corrected by multiplying the jitter correction data stored in the correction data storage unit 87 or the reference correction data storage unit 95 by a correction coefficient. The correction data may be used to perform jitter correction of the sample digital data.

In the embodiment shown in FIGS. 10 and 11, the jitter correction data generation sample 80 has substantially the same size as the microarray and has a regular basic pattern formed on the entire surface. Is used to generate jitter correction data capable of correcting all even lines of the digital data of the sample 22, but by increasing the moving pitch in the sub-scanning direction, the digital data of the sample 22 having four or more lines The jitter correction data is generated for each data area of
7 or the reference correction data storage unit 95, and the jitter correction of the digital data of the sample 22 may be performed by using different jitter correction data for each area of the digital data of the sample 22.

Further, in the embodiment shown in FIGS. 12 and 13, the reference correction data storage section 95 stores the jitter correction data generated based on the jitter correction data generation sample 80, and stores the sample. Prior to generation of the digital data of the sample carrier 22, when the jitter correction data is not generated according to the regular basic shading pattern 90 formed on one side surface 50a of the frame body 50 of the sample carrier 21, the jitter correction data The jitter in the digital data of the sample 22 is corrected based on the jitter correction data generated based on the sample 80 and stored in the reference correction data storage unit 95. Prior to data generation, the frame body of the sample carrier 21 0, each time the jitter correction data is generated in accordance with the regular basic shading pattern 90 formed on the one side surface 50a, the generated jitter correction data is used to store the jitter stored in the reference correction data storage unit 95. It is also possible to configure so as to overwrite the correction data.

Also, in the above embodiment, the confocal switching member 31 has three pinholes 32 having different diameters.
a, 32b, 32c are formed and a number of spots of the sample selectively labeled with a fluorescent dye are scanned by a laser beam 4 on a microarray formed on a slide glass plate to excite the fluorescent dye. When the fluorescence emitted from the fluorescent dye is photoelectrically detected to generate data for biochemical analysis, the pinhole 32a is provided with the position information of the radiolabeled substance obtained by exposing the stimulable phosphor layer. The stimulable phosphor layer of the stimulable phosphor sheet on which is recorded is scanned by the laser light 4 to excite the stimulable phosphor, and the stimulable phosphor emitted from the stimulable phosphor is photoelectrically irradiated. To generate data for biochemical analysis,
2b is electrophoresed on the gel support and scans with a laser light 4 a fluorescent sample using a gel support containing a sample selectively labeled with a fluorescent dye,
When the fluorescent dye is excited and the fluorescence emitted from the fluorescent dye is photoelectrically detected to generate data for biochemical analysis, the pinholes 32c are used, but the confocal switching member 31 is used. And pinholes 32a, 3
A large number of spots of the sample which form only 2b and are selectively labeled with a fluorescent dye are scanned by a laser beam 4 on a microarray formed on a slide glass plate to excite the fluorescent dye, When photoelectrically detecting the fluorescence emitted from the phosphor and generating data for biochemical analysis, the fluorescence 25 is received via the pinhole 32a, and the stimulable luminescence emitted from the stimulable phosphor layer is emitted. When photoelectrically detecting 25 and generating data for biochemical analysis, the photostimulable light is received via the pinhole 32b, and the fluorescence 25 emitted from the gel support is photoelectrically detected. When generating data for biochemical analysis, the confocal switching member 31 may be retracted from the optical path of the fluorescent light 25 to increase the amount of light received by the photomultiplier 33, And, a confocal switching member 31, only to form pinholes 32a, a number of spots of selectively labeled sample by fluorescent dye, a microarray formed on a slide glass plate, the laser beam 4
By scanning, the fluorescent dye is excited, the fluorescence emitted from the fluorescent dye is photoelectrically detected, and only when data for biochemical analysis is generated, via the pinhole 32a,
The fluorescent light 25 is received, and the photostimulable light 25 emitted from the stimulable phosphor layer is photoelectrically detected to generate the data for biochemical analysis, and the fluorescent light 25 emitted from the gel support is photoelectrically detected. When the data for biochemical analysis is to be detected and detected, the confocal switching member 31 can be retracted from the optical path of the fluorescent light 25 so that the amount of light received by the photomultiplier 33 can be increased.

Further, in the embodiment shown in FIGS. 1 to 9 and the embodiments shown in FIGS. 10 and 11, a chromium vapor-deposited film 81 is formed on the surface of the color glass filter to form the color glass filter. Generates the jitter correction data generating member 80 having a regular basic pattern of, but when it is irradiated with the laser beam 4, it is excited,
It is sufficient that a regular basic pattern of a fluorescent substance that emits fluorescence is formed, and it is not always necessary to form the chromium deposited film 81 on the surface of the color glass filter to form the regular basic pattern of the color glass filter. Not necessary.

In the embodiment shown in FIGS. 1 to 9 and the embodiments shown in FIGS. 10 and 11, a chromium vapor-deposited film 81 is formed on the surface of a color glass filter to form a color glass filter. A jitter correction data generation member 80 having a regular basic pattern of FIG.
In the embodiment shown in FIG. 2 and FIG. 13, a regular basic shading pattern 90 is formed on a part of one side face 50a of the frame body 50 constituting the sample carrier 21, thereby providing the jitter correction data. Generation member 8
0, but in the embodiment shown in FIGS. 1 to 9 and the embodiments shown in FIGS. 10 and 11, a chromium vapor-deposited film 81 is formed on the surface of a color glass filter to form a color filter. Instead of using the member 80 for generating jitter correction data having a regular basic pattern of a glass filter, as in the embodiment shown in FIGS.
The jitter correction data is generated by photoelectrically detecting the laser beam 4 reflected from the surface of the jitter correction data generation member 80 using the jitter correction data generation member 80 having the regular basic shading pattern formed thereon. In the embodiment shown in FIGS. 12 and 13, a part of one side surface 50a of the frame body 50 constituting the sample carrier 21 is provided with a regular basic gray pattern 90. Is formed, one side surface 50a of the frame body 50 constituting the sample carrier 21 is formed.
For example, by forming a fluorescent material layer on a part of the chromium, and forming a chromium deposited film 81 on the surface of the fluorescent material layer,
It is also possible to form a regular basic pattern of the fluorescent substance, photoelectrically detect the fluorescence 25 emitted from the regular basic pattern of the fluorescent substance, and generate jitter correction data.

Further, in each of the above embodiments, the bidirectional scanning scanner is provided with the sampling stage 20.
Is reciprocated in the main scanning direction by the main scanning motor 43 and is moved in the sub scanning direction by the sub scanning motor 47 so that the entire surface of the sample 22 is
The bidirectional scanning scanner is configured so that the optical head 15 is reciprocated in the main scanning direction and is moved in the sub-scanning direction. , The entire surface of the sample 22 can be scanned.

[0334]

According to the present invention, a jitter correction method capable of correcting jitter in a bidirectional scanning scanner simply and at low cost, and a jitter correction method easily and at low cost. It is possible to provide a high-resolution bidirectional scanning scanner capable of generating a correction data for correcting a jitter easily and at low cost, and a sample carrier for the bidirectional scanning scanner capable of generating correction data for correcting jitter. .

[Brief description of the drawings]

FIG. 1 is a schematic perspective view of a bidirectional scanning scanner according to a preferred embodiment of the present invention.

FIG. 2 is a schematic perspective view of a microarray.

FIG. 3 is a schematic front view of a confocal switching member.

FIG. 4 shows a scanning mechanism of a sample stage.
FIG. 3 is a schematic perspective view illustrating details of a main scanning mechanism.

FIG. 5 is a schematic perspective view of a sample carrier set on a sample stage holding a microarray using a slide glass plate as a carrier. The sample carrier is placed from the back side, that is, placed on the sample stage. It is a drawing viewed from the side where

FIG. 6 is a block diagram showing a detection system, a drive system, an input system, and a control system of the bidirectional scanning scanner according to the preferred embodiment of the present invention.

FIG. 7 is a schematic front view of a member for generating jitter correction data used when generating jitter correction data for correcting jitter.

FIG. 8 is a block diagram of a data processing device of a bidirectional scanning scanner according to a preferred embodiment of the present invention.

FIG. 9 is a diagram showing an example in which a correction data generation sample is scanned by a laser beam for one round trip so that a color glass filter is excited, and fluorescence emitted from the surface of the color glass filter is photomultiplied. 5 is a diagram in which digital data of a reference pattern, which is photoelectrically detected by a prior, is digitized by an A / D converter, and input to a correction data generation unit of the data processing device, is imaged.

FIG. 10 is a schematic front view of a member for generating jitter correction data used for generating jitter correction data in a bidirectional scanning scanner according to another preferred embodiment of the present invention.

FIG. 11 is a diagram illustrating a configuration in which a color glass filter is excited by scanning a correction data generation sample with laser light, and fluorescence emitted from the surface of the color glass filter is photoelectrically detected by a photomultiplier; The digital data of the (2N-1) -th and 2N-th lines of the reference pattern, which are digitized by the A / D converter and input to the correction data generation unit of the data processing device, are imaged. .

FIG. 12 is a schematic perspective view of a sample carrier set on a sample stage of a bidirectional scanning scanner according to another preferred embodiment of the present invention.

FIG. 13 is a block diagram of a data processing device of a bidirectional scanning scanner according to another preferred embodiment of the present invention.

[Explanation of symbols]

 REFERENCE SIGNS LIST 1 first laser excitation light source 2 second laser excitation light source 3 third laser excitation light source 4 laser light 5 collimator lens 6 mirror 7 first dichroic mirror 8 second dichroic mirror 9 collimator lens 10 collimator lens 15 optical head Reference Signs List 16 mirror 17 hole 18 perforated mirror 19 lens 20 sample stage 21 sample carrier 22 sample 23 dropped cDNA 25 fluorescence or stimulating light 27 filter unit 28a, 28b, 28c, 28d filter 29 mirror 30 lens 31 confocal switching member 32a, 32b, 32c, 32d, 32e Pinhole 33 Photomultiplier 34 A / D converter 35 Data processing device 40 Movable substrate 41, 41 A pair of guide rails 42 Slide member 43 Main scanning module 43a Output shaft of main scanning motor 44 Pulley 45 Timing belt 46 Rotary encoder 47 Sub-scanning motor 50 Frame body 51, 52, 53, 54, 55 Opening 51a, 52a, 53a, 54a, 55a Leaf spring 51b, 52b , 53b, 54b, 55b Leaf spring 60, 61, 62, 63, 64, 65 Plate member 70 Carrier sensor 71 Filter unit motor 72 Switching member motor 73 Keyboard 75 Control unit 80 Jitter correction data generation member 81 Chrome evaporated film 85 Data Storage unit 86 Correction data generation unit 87 Correction data storage unit 88 Data processing unit 90 Regular basic pattern 95 Reference correction data storage unit

Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat II (reference) G01N 37/00 102 G06T 1/00 460A 5C072 G06T 1/00 460 C12M 1/00 A 5C077 H04N 1/40 H04N 1/04 E // C12M 1/00 1/40 101Z C12N 15/09 C12N 15/00 FF term (reference) 2G043 AA03 AA04 BA16 CA03 DA02 DA05 DA09 EA01 EA19 FA01 FA06 GA04 GA08 GB18 GB19 GB21 HA01 HA02 HA09 JA03 KA09 LA02 MA04 NA06 2G058 AA09 CC09 CD11 GA01 GD03 4B024 AA11 AA19 CA01 CA04 CA11 CA12 HA12 4B029 AA07 AA23 BB15 BB16 BB17 BB20 CC03 CC08 FA15 5B047 AA17 AB02 BA01 BB08 CA06 CB07 DA10 DB01 DC06 DC11 5C0701 A02 BA01 VA02 VA01 VA02 MM08 MM27 MP01 PP05 PP57 PP71 PQ22 RR01 SS01

Claims (36)

[Claims] A laser beam is moved relative to a sample stage on which a sample is set and a laser beam so as to reciprocate in the main scanning direction, and is moved in a sub-scanning direction orthogonal to the main scanning direction. The method for correcting jitter in a bidirectional scanning scanner configured to move the sample, scan the sample with the laser light, and photoelectrically detect light emitted from the sample, The sample for correction data generation, which is fixed relative to the stage and has a regular pattern, is scanned by the laser light, and light emitted from the sample for correction data generation is photoelectrically detected. Detecting, generating analog data, digitizing the analog data, generating digital data for generating correction data, Based on the digital data for generating the positive data, data that minimizes the deviation of the digital data for generating the correction data for each scan line is determined as jitter correction data and stored in the memory of the bidirectional scanning scanner. Then, a sample labeled with a labeling substance is placed on the sample stage, the sample is scanned by the laser light, the labeling substance is excited, and light emitted from the labeling substance is photoelectrically converted. Detecting, generating analog data, and correcting the digital data of the sample obtained by digitizing the analog data using the jitter correction data stored in the memory of the bidirectional scanning scanner. A method for correcting jitter in a bidirectional scanning scanner. 2. The method according to claim 1, wherein the sample stage and the laser light are reciprocated once in the main scanning direction to scan the correction data generation sample with the laser light. Photoelectrically detect the light emitted from the sample to generate analog data,
The analog data is digitized to generate digital data for generating correction data including digital data on the first line corresponding to the forward path and digital data on the second line corresponding to the return path, Data that minimizes the deviation of the digital data on the second line with respect to the digital data on the first line is determined as jitter correction data based on the digital data of the first line, and the memory of the bidirectional scanning scanner is determined. And correcting the digital data of the sample corresponding to an even-numbered scan line using the jitter correction data stored in the memory of the bidirectional scanning scanner. A method for correcting jitter in the bidirectional scanning scanner as described in the above. 3. The method according to claim 1, wherein the sample stage and the laser beam are relatively moved in a main scanning direction and a sub-scanning direction, and the laser beam scans a sample for the correction data generation. Detects light emitted from the sample for photoelectrically, generates analog data, digitizes the analog data, generates digital data for generating correction data, and converts the digital data for generating the correction data into digital data. Based on (2N-1)
Digital data of the Nth line (N is an integer of 1 or more)
Are determined as jitter correction data, and are stored in the memory of the bidirectional scanning scanner, and are stored in the memory of the bidirectional scanning scanner. 2. The method for correcting jitter in a bidirectional scanning scanner according to claim 1, wherein the digital data of the sample corresponding to the 2N-th line is corrected using the obtained jitter correction data. 4. The sample stage and the laser beam are relatively moved in a sub-scanning direction at a large moving pitch, and the jitter correction data is converted into data of digital data of the sample including four or more lines. The method for correcting jitter in a bidirectional scanning scanner according to claim 1, wherein the jitter is generated for each area and stored in the memory of the bidirectional scanning scanner. 5. The method according to claim 1, wherein the regular pattern formed on the correction data generation sample is formed of a fluorescent material, and the laser light excites the fluorescent material, and the laser light excites the regular pattern. 5. A method for correcting jitter in a bidirectional scanning scanner according to claim 1, wherein the emitted fluorescence is photoelectrically detected to generate digital data for generating the correction data. . 6. The method according to claim 1, wherein the regular pattern formed on the correction data generation sample is formed by a visible shading pattern, and the laser beam reflected by the correction data generation sample is photoelectrically detected. 5. The method for correcting jitter in a bidirectional scanning scanner according to claim 1, wherein the digital data for generating the correction data is generated. 7. The correction data generating sample is scanned by the laser light by moving the sample stage in the sub-scanning direction while reciprocating in the main scanning direction, and scanning the correction data generating sample. Detecting light emitted from photoelectrically, generates analog data, digitizes the analog data, generates digital data for correction data generation, based on the digital data for correction data generation, Data that minimizes the amount of deviation of the digital data for the correction data for each scanning line is determined as jitter correction data, stored in the memory of the bidirectional scanning scanner, and labeled on the sample stage with a labeling substance. The sample stage is placed, and the sample stage is reciprocated in the main scanning direction while the sub-scanning is performed. Moving in the direction, scanning the sample with the laser light, exciting the labeling substance, photoelectrically detecting light emitted from the labeling substance, generating analog data, and generating the analog data. 7. The digital data of a sample obtained by digitizing is corrected using the jitter correction data stored in the memory of the bidirectional scanning scanner. A method for correcting jitter in the bidirectional scanning scanner as described in the above. 8. The correction data generating sample is scanned by the laser light by moving the laser light in the sub-scanning direction while reciprocating in the main scanning direction to scan the correction data generating sample. Detecting light emitted from photoelectrically, generates analog data, digitizes the analog data, generates digital data for correction data generation, based on the digital data for correction data generation, Data that minimizes the amount of deviation of the digital data for the correction data for each scanning line is determined as jitter correction data, stored in the memory of the bidirectional scanning scanner, and labeled on the sample stage with a labeling substance. Placed on the sample, the laser light, while reciprocating in the main scanning direction,
Move in the sub-scanning direction, scan the sample with the laser light, excite the labeling substance, photoelectrically detect light emitted from the labeling substance, generate analog data, and generate the analog data. 7. The digital data of a sample obtained by digitizing data is corrected using the jitter correction data stored in the memory of the bidirectional scanning scanner.
The method for correcting jitter in the bidirectional scanning scanner according to any one of the above. 9. A jitter correction data is generated according to a relative moving speed of the sample stage and the laser beam in the main scanning direction, and is stored in the memory of the bidirectional scanning scanner. 9. The method for correcting jitter in a bidirectional scanning scanner according to claim 1, wherein: 10. The jitter correction data is generated by relatively moving the sample stage and the laser beam in the main scanning direction at a specific moving speed, and the jitter correction data is stored in the memory of the bidirectional scanning scanner. When the sample stage and the laser light are relatively moved in the main scanning direction at a moving speed different from the specific moving speed to generate digital data of the sample, the bidirectional scanning scanner is used. 9. The apparatus according to claim 1, wherein the jitter correction data stored in the memory is corrected to generate jitter correction data, and the digital data of the sample is corrected. A method for correcting jitter in a directional scanning scanner. 11. The memory of the bidirectional scanning scanner, wherein the sample stage and the laser beam respectively generate jitter correction data according to a pixel pitch when the laser beam is relatively moved in a main scanning direction. 11. The method for correcting jitter in a bidirectional scanning scanner according to claim 1, wherein the jitter is stored in the bidirectional scanning scanner. 12. The sample stage and the laser beam are relatively moved at a specific pixel pitch in the main scanning direction to generate jitter correction data and store the jitter correction data in the memory of the bidirectional scanning scanner. When the sample stage and the laser beam are relatively moved in the main scanning direction at a pixel pitch different from the specific pixel pitch to generate digital data of the sample, the bidirectional scanning scanner is used. 11. The apparatus according to claim 1, wherein the jitter correction data stored in the memory is corrected to generate jitter correction data, and the digital data of the sample is corrected. 12. A method for correcting jitter in a directional scanning scanner. 13. The method of correcting a jitter in a bidirectional scanning scanner according to claim 1, wherein jitter correction data is generated as necessary and stored in said memory. 14. The method according to claim 14, wherein the sample stage is set to:
A regular pattern formed on the surface of a sample carrier having a sample holding unit for holding the sample is scanned by the laser light, and light emitted from the correction data generation sample is detected by the photodetector. Detects photoelectrically, generates analog data, digitizes the analog data with the A / D converter, generates digital data for generating correction data, and generates the correction data based on the digital data for generating correction data. Data that minimizes the amount of deviation for each scan line of digital data for data generation,
14. The method for correcting jitter in a bidirectional scanning scanner according to claim 1, wherein the data is determined as jitter correction data and stored in the memory. 15. The method according to claim 14, wherein the regular pattern is formed outside the sample holder of the sample carrier. 16. A sample labeled with a fluorescent substance is placed on the sample stage, and the sample is scanned with a laser beam to excite the fluorescent substance and to emit fluorescence emitted from the fluorescent substance. The photoelectrically detected analog data is generated, and the digital data of the sample obtained by digitizing the analog data is corrected using the jitter correction data stored in the memory of the bidirectional scanning scanner. The method for correcting jitter in a bidirectional scanning scanner according to any one of claims 1 to 15, wherein: 17. A stimulable phosphor sheet provided with a stimulable phosphor layer labeled with a radioactive label substance as the sample on the sample stage, and the stimulable phosphor sheet is irradiated with a laser beam. By scanning the body layer, exciting the stimulable phosphor contained in the stimulable phosphor layer, photoelectrically detecting the stimulable light emitted from the stimulable phosphor,
The digital data of a sample obtained by generating analog data and digitizing the analog data is corrected by using the jitter correction data stored in the memory of the bidirectional scanning scanner. 16. The method for correcting jitter in a bidirectional scanning scanner according to any one of 1 to 15. 18. A sample stage on which a sample labeled with a labeling substance is set, and at least one laser excitation light source that emits laser light, wherein the sample stage and the laser light are arranged in a main scanning direction.
In order to reciprocate, they are relatively moved, and are relatively moved in a sub-scanning direction orthogonal to the main scanning direction. The laser beam scans the sample and is emitted from the sample. A bidirectional scanning scanner configured to photoelectrically detect light, further comprising: a photodetector that photoelectrically receives light emitted from the labeling substance and generates analog data; and The laser light emitted from one laser excitation light source is condensed on the sample stage, and the light emitted from the labeling substance is condensed by an optical condensing optical system that guides the light to the photodetector. An A / D converter for digitizing the generated analog data, a data processing device, and a memory, wherein the data processing device is configured to: A sample for correction data generation, which is fixed and regularly patterned, is scanned by laser light emitted from the at least one laser excitation light source and emitted from the sample for correction data generation. Light is photoelectrically detected by the photodetector to generate analog data,
Based on the digital data generated by the D converter and generated for the correction data, the data for minimizing the deviation of the digital data for the correction data for each scan line is determined as the jitter correction data. Then, stored in the memory, placed on the sample stage, a sample labeled with a labeling substance,
The labeling substance is excited by being scanned by the laser light emitted from the at least one laser excitation light source,
The light emitted from the labeling substance is photoelectrically detected by the photodetector, and analog data is generated.
The digital data of the sample generated and digitized by the A / D converter is corrected by using the jitter correction data stored in the memory. Scanning scanner. 19. The data processing device, wherein the sample stage and the laser beam are relatively positioned in a main scanning direction.
One reciprocation, the laser light scans the sample for correction data generation, the light emitted from the sample for correction data generation, by the photodetector,
The analog data that has been photoelectrically detected and generated is digitized by the A / D converter, and the generated digital data on the first line corresponding to the forward path and the first digital data corresponding to the return path are generated. Based on the digital data for generating the correction data consisting of the digital data of, the data that minimizes the deviation of the digital data of the second line with respect to the digital data of the first line is determined as jitter correction data. 19. The apparatus according to claim 18, wherein the digital data of the sample corresponding to the even-numbered scan line is corrected using the jitter correction data while being stored in the memory. Directional scanning scanner. 20. The data processing apparatus, wherein the sample stage and the laser beam are relatively moved in a main scanning direction and a sub-scanning direction to scan a sample for generating the correction data. Light emitted from the sample for generation is photoelectrically detected by the photodetector, and the generated analog data is digitized by the A / D converter to generate the generated correction data. Based on the digital data, the data that minimizes the deviation of the 2N-th line digital data with respect to the (2N-1) -th line digital data (N is an integer of 1 or more) is determined as jitter correction data. Then, while being stored in the memory, the jitter correction data is used to store the sample corresponding to the 2Nth line. The bidirectional scanning scanner according to claim 18, wherein the scanner is configured to correct the digital data. 21. The data processing device, wherein the sample stage and the laser beam are relatively moved at a large moving pitch in a sub-scanning direction, and the jitter correction data includes four or more lines. 19. The bidirectional scanning scanner according to claim 18, wherein the bidirectional scanning scanner is configured to generate and store the digital data for each sample in the memory. 22. The regular pattern formed on the sample for generating the correction data is formed of a fluorescent substance, and the data processing device excites the fluorescent substance by the laser beam, and performs the correction. 22. Bidirectional scanning according to any one of claims 18 to 21, wherein fluorescence emitted from a sample for data generation is photoelectrically detected to generate digital data for generating the correction data. Scanner. 23. The laser beam reflected by the correction data generation sample, wherein the regular pattern formed on the correction data generation sample is formed by a visible shading pattern, and the data processing apparatus reflects the laser light reflected by the correction data generation sample. 18. The digital data for generating the correction data is generated by photoelectrically detecting the correction data.
2. The bidirectional scanning scanner according to claim 1. 24. A data processing apparatus, wherein the data processing device generates jitter correction data according to a relative moving speed of the sample stage and the laser beam in a main scanning direction, and stores the data in the memory. The bidirectional scanning scanner according to any one of claims 18 to 23, wherein the scanning is performed. 25. When the sample stage and the laser beam are relatively moved in the main scanning direction at a specific moving speed, the data processing device generates jitter correction data and When the sample stage is moved in the main scanning direction at a moving speed different from the specific moving speed, and the digital data of the sample is generated, the data processing device stores the digital data in the memory. 24. The apparatus according to claim 18, wherein the jitter correction data stored in the memory is corrected to generate jitter correction data, and the digital data of the sample is corrected. Bi-directional scanning scanner. 26. The data processing device, according to a pixel pitch when the sample stage and the laser beam are relatively moved in a main scanning direction, respectively, generates jitter correction data, The bidirectional scanning scanner according to any one of claims 18 to 25, wherein the bidirectional scanning scanner is configured to be stored in the memory of the bidirectional scanning scanner. 27. When the sample stage and the laser beam are relatively moved at a specific pixel pitch in the main scanning direction, the data processing device generates jitter correction data, and When the digital data of the sample is generated by moving the sample stage in the main scanning direction at a pixel pitch different from the specific pixel pitch, the data processing apparatus 26. The apparatus according to claim 18, wherein the jitter correction data stored in the memory is corrected to generate jitter correction data, and the digital data of the sample is corrected. Bi-directional scanning scanner. 28. A regular pattern of the fluorescent substance is formed on a surface of the sample stage on the side of the at least one laser excitation light source, and the data processing device is stored in the memory. The bidirectional scanning scanner according to any one of claims 18 to 27, wherein the jitter correction data is configured to be updated. 29. The data processing device, as the sample, is mounted on the sample stage and scans a sample labeled with a fluorescent substance with a laser beam to excite the fluorescent substance, Fluorescence emitted from the, photoelectrically detected by the photodetector,
It is configured that the generated analog data is digitized by the A / D converter, and the generated digital data of the sample is corrected using the jitter correction data stored in the memory. The bidirectional scanning scanner according to any one of claims 18 to 28, wherein: 30. The stimulable fluorescent light of a stimulable phosphor sheet comprising a stimulable phosphor layer which is mounted on the sample stage as the sample as the sample and is labeled with a radioactive labeling substance. The body layer is scanned by a laser beam to excite the stimulable phosphor contained in the stimulable phosphor layer, and the photostimulable light emitted from the stimulable phosphor is detected by the light detection. A / D converter converts the generated analog data into digital data by using the A / D converter, and generates the digital data of the sample using the jitter correction data stored in the memory. 29. The bidirectional scanning scanner according to claim 18, wherein the bidirectional scanning scanner is configured to perform correction. 31. The at least one laser excitation light source, wherein the data processing device is set on the sample stage and a regular pattern formed on a surface of a sample carrier provided with a sample holding unit for holding the sample is provided. Scanning by the laser light emitted from the light source, light emitted from the sample for generating the correction data is photoelectrically detected by the photodetector to generate analog data, and the A / D converter Based on the generated digital data for correction data generation, data for minimizing the deviation of the digital data for correction data generation for each scan line is determined as jitter correction data, 31. The apparatus according to claim 18, wherein said memory is configured to be stored in said memory. A bidirectional scanning scanner according to claim 1. 32. The bidirectional scanning scanner according to claim 31, wherein the regular pattern is formed outside the sample holder of the sample carrier. 33. A sample carrier mounted on a sample stage, comprising a sample holding portion capable of holding a sample, wherein a regular pattern is formed on a surface on the sample stage side. Sample carrier. 34. The sample carrier according to claim 33, wherein the regular pattern is formed outside the sample holder of the sample carrier. 35. The method according to claim 33, wherein the regular pattern is formed of a fluorescent material.
5. The sample carrier according to 4. 36. The method according to claim 33, wherein the regular pattern is formed by a visible shading pattern.
Or the sample carrier of 34.