JP2002181696A - Method and apparatus for evaluating kinetic property of water - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method and apparatus for evaluating the kinetic property of water capable of simplifying evaluating operation and increasing the efficiency of evaluation. SOLUTION: The method for evaluating the kinetic property of water includes applying a monitor beam to sample water while applying a magnetic field to the sample water; receiving the monitor beam that has propagated through the sample water; measuring predetermined spectroscopic properties such as the absorbency or Faraday rotation angle of the sample water based on the received monitor beam; and evaluating the kinetic property of the sample water on the basis of the predetermined spectroscopic properties of the sample water measured with and without the application of the magnetic field.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an evaluation method and an evaluation apparatus for evaluating the motility of water in a living body or the like.

[0002]

2. Description of the Related Art A living body is composed of about 30% of a biopolymer and about 70%.
% Of water. Since water molecules have electrical polarity, water molecules in a living body hydrogen bond with surrounding water molecules, biopolymers, and the like. Water bound by water molecules
It is called highly mobile water (free water) because it repeats binding and separation in a very short time. On the other hand, water bound to a biopolymer such as a membrane or a protein stays at the place for a relatively long time, and is therefore called water with low mobility (bound water).

As described above, it is known that there are different states of water, such as free water and combined water, and that the motility is different. Here, it is extremely important to elucidate the relationship between biological activity and water motility in order to deepen knowledge about living organisms. Therefore, a method that can accurately evaluate water motility is required. Can be

[0004] Conventionally, the mobility of water has been measured by NMR (Nuclear
(Magnetic Resonance) It is possible to analyze and evaluate the relaxation time, but especially when evaluating the motility of water inside cells, a cell-level spatial resolution like a microscope is required. , And “microscopic NMR”, which is a method based on NMR with improved spatial resolution.

[0005]

However, the above-mentioned conventional method using NMR requires a complicated analysis operation, and requires a long time measurement in order to obtain a spatial resolution, and the evaluation efficiency is low. There was a problem.

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned problems of the prior art, and has been made in consideration of the above-described problems. It is intended to provide a device.

[0007]

Means for Solving the Problems The present inventors have conducted intensive studies to achieve the above object, and found that when a magnetic field is applied to water, the spectroscopic properties of water, such as absorbance and Faraday rotation angle, change. Was found. In addition, the inventor has found from an experiment using a gel that there is a correlation between such a change in the spectroscopic properties of water and the mobility of water. And
The inventors have found that it is possible to evaluate the motility of water by measuring the change in spectroscopic properties between a state where a magnetic field is applied to water and a state where no magnetic field is applied to the water. Reached.

That is, in the method for evaluating the mobility of water according to the present invention, in a state where a magnetic field is applied to the sample water, the sample water is irradiated with monitor light, and the monitor light transmitted through the sample water is received. The predetermined spectroscopic properties of the sample water are measured based on the monitored light, and the predetermined spectroscopic properties of the sample water measured with the magnetic field applied and the predetermined spectroscopy of the sample water without the magnetic field applied It is characterized in that the motility of the sample water is evaluated based on the characteristic properties.

In this method for evaluating the mobility of water, the mobility of the sample water is evaluated based on a change in predetermined spectroscopic properties caused by applying a magnetic field to the sample water. As described above, since the spectroscopic properties of the sample water are measured using light, the analysis work is simplified as compared with the case of using NMR,
In addition, high spatial resolution can be measured in a short time, and the efficiency of evaluation can be improved.

In the method for evaluating the mobility of water according to the present invention, the absorbance at a predetermined wavelength is measured as a predetermined spectroscopic property of the sample water, and the absorbance measured with a magnetic field applied and the absorbance with no magnetic field applied are measured. The method may be characterized in that the motility of the sample water is evaluated based on the difference between. In this way, the mobility of the sample water is evaluated based on the difference between the absorbance measured in the state where the magnetic field is applied and the absorbance measured in the state where the magnetic field is not applied.

In the method for evaluating the mobility of water according to the present invention, linearly polarized light is used as the monitor light, and the deflection angle of the monitor light propagated through the sample water is measured as a predetermined spectroscopic property of the sample water. The motility of the sample water may be evaluated based on a difference between a deflection angle measured in a state where a magnetic field is applied and a deflection angle in a state where no magnetic field is applied. In this way, the motility of the sample water is evaluated based on the difference between the deflection angle of the monitor light measured with the magnetic field applied and the deflection angle of the monitor light without the magnetic field applied.

Further, in the method for evaluating the mobility of water according to the present invention, linearly polarized light is used as monitor light, and at least one of monitor light before irradiating the sample water and monitor light after propagating through the sample water. The light having a predetermined deflection angle measured in a state where a magnetic field is applied is measured by measuring the intensity of light having a predetermined deflection angle among monitor light scattered as predetermined spectroscopic properties of sample water. The mobility of the sample water may be evaluated based on a ratio between the intensity of the sample water and the intensity of light having a predetermined deflection angle in a state where no magnetic field is applied. With this configuration, the motion of the sample water is determined based on the ratio between the intensity of the light having the predetermined deflection angle measured in the state where the magnetic field is applied and the intensity of the light having the predetermined deflection angle in the state where the magnetic field is not applied. Sex evaluation is performed.

In the method for evaluating the mobility of water according to the present invention, it is preferable that the intensity of the applied magnetic field be 200 mT or more.
If the strength of the applied magnetic field is smaller than 200 mT, the measurement accuracy of a change in a predetermined spectroscopic property that occurs when a magnetic field is applied to the sample water, and consequently, the accuracy of the evaluation of the mobility of the sample water W tends to decrease. is there.

The apparatus for evaluating the mobility of water according to the present invention is an apparatus for suitably implementing the above-described method for evaluating the mobility of water according to the present invention. (1) A magnetic field is applied to sample water. (2) a light source device for irradiating the sample water with monitor light, and (3) a light receiving device for receiving the monitor light propagated in the sample water.
(4) An arithmetic unit for calculating a difference in absorbance at a predetermined wavelength of the sample water between a state where a magnetic field is applied to the sample water and a state where no magnetic field is applied to the sample water based on the monitor light received by the light receiving device. It is characterized by.

In the water kinetic evaluation apparatus according to the present invention, the light receiving device may include a filter that transmits light of a predetermined wavelength, and a light receiving element that receives light transmitted through the filter. Further, the water motility evaluation device according to the present invention includes (1) a magnetic field applying device for applying a magnetic field to the sample water, and (2) a linearly polarized monitor light for irradiating the sample water with the monitor light. A light source device, (3) a deflection angle detection device for receiving the monitor light propagated in the sample water and detecting its deflection angle, and (4) a deflection angle of the monitor light detected by the deflection angle detection device. And a calculating device for calculating a difference in deflection angle of monitor light that has propagated in the sample water when a magnetic field is applied to the sample water and when no magnetic field is applied to the sample water.

[0016] The water motility evaluation apparatus according to the present invention comprises:
(1) a magnetic field applying device for applying a magnetic field to the sample water; (2) a light source device for irradiating the sample water with linearly polarized monitor light; and (3) before irradiating the sample water. A scatterer for scattering at least one of the monitor light and the monitor light after propagating in the sample water,
(4) a polarizing element for passing only light having a predetermined deflection angle out of monitor light propagated in the sample water scattered by the scatterer, and (5) a light receiving element for receiving light passing through the polarizing element. And (6) an arithmetic unit for calculating a ratio of the intensity of light having a predetermined deflection angle between a state where a magnetic field is applied to the sample water and a state where no magnetic field is applied to the sample water, based on the light received by the light receiving element. It is characterized by the following.

Further, the water motility evaluation apparatus according to the present invention may be characterized in that it is provided with a positioning means for positioning the sample water.

[0018]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Preferred embodiments of the method and apparatus for evaluating water motility according to the present invention will be described below with reference to the accompanying drawings. In the drawings, the same elements will be denoted by the same reference symbols, without redundant description.

(First Embodiment) First, the water motility evaluation method according to the first embodiment (hereinafter also referred to as "evaluation method") and a water motility evaluation device (hereinafter also referred to as "evaluation device"). Before describing (), the principle of evaluating the motility of water in the present embodiment will be described with reference to FIGS. 1 and 2.

Water has a center wavelength λ ₀ = 960, 1450, 1
It has a large absorbance near 960 and 3000 nm. In FIG. 1, a solid line L1 indicates an absorption spectrum of water near the center wavelength λ ₀ . When a magnetic field is applied to water having such an absorption spectrum, as shown by a broken line L2,
The inventor has found that the absorption spectrum of water slightly shifts to the shorter wavelength side. When measuring the differential absorbance is the difference in absorbance before and after the application of a magnetic field, as shown in FIG. 2, the maximum value A ₁ at the wavelength lambda _1, and the differential absorption spectrum having the minimum value A ₂ at the wavelength lambda ₂ Become.
Then, the maximum amplitude ΔA of the differential absorbance is represented as A ₁ + | A ₂ |. When the center wavelength λ ₀ of the solid line L1 in FIG. 1 is 1450 nm, the wavelengths λ ₁ and λ ₂ at which the maximum value A ₁ and the minimum value A ₂ of the differential absorbance take, for example, are 1420 nm and 1480 nm, respectively. Becomes

Here, the gel has the property that the higher the concentration, the less water (free water) with high mobility and the more water with low mobility (bound water). Therefore, the inventors prepared a plurality of samples having different gel concentrations and examined the relationship between the gel concentration and the maximum amplitude ΔA of the differential absorbance. Then, as shown in FIG. 3, as the gel concentration increased, the change in absorbance before and after the application of the magnetic field (the maximum amplitude ΔA of the differential absorbance) tended to decrease. As described above, there is a correlation between the gel concentration and the maximum amplitude ΔA of the differential absorbance, and it has been found that by measuring the maximum amplitude ΔA of the differential absorbance, it is possible to evaluate the motility of water. . For example, when ΔA is large, it corresponds to the case where the gel concentration is low, and it can be evaluated that the mobility of water is high (there is a lot of free water). On the other hand, a small ΔA corresponds to a high gel concentration,
It can be evaluated that the mobility of water is low (the amount of bound water is large).

An evaluation method and an evaluation device according to the present embodiment described below are based on the above-described principle.

FIG. 4 is a diagram schematically showing the configuration of the evaluation device according to the present embodiment. As shown in the figure, the evaluation device 10 includes a constant temperature chamber 12 containing sample water (sample water) W.
A light source device 14 for irradiating the sample water W with monitor light, first and second light receiving devices 16 and 18 for receiving monitor light propagated in the sample water W, and a spatial resolution at a cell level. And a microscope device 20 for obtaining the same.

The constant temperature chamber 12 is a hermetically sealed container capable of maintaining the internal temperature substantially constant by a constant temperature control circuit 56 which is electrically connected thereto. The container 11 is accommodated. The sample container 11 has a reflecting plate 22 for reflecting light at the bottom, and a cover glass 24 provided on the upper surface thereof so as to be opened and closed.
Covered by Further, the constant temperature chamber 12 has an electric stage (positioning means) 26 for positioning the sample container 11. The electric stage 26 is electrically connected to the stage control circuit 60, and the electric stage 26 is driven and controlled by an electric signal from the stage control circuit 60 to position the sample container 11. Further, the constant temperature chamber 12 has a magnetic field application device 28 for applying a magnetic field to the sample water W. The magnetic field application device 28 is electrically connected to a magnetic field application control circuit 58, and the magnetic field application device 28 is driven and controlled by an electric signal from the magnetic field application control circuit 58 so that a magnetic field is applied to the sample water W in the sample container 11. Applied.

The light source device 14 has a light source 30 for emitting monitor light for irradiating the sample water W, and a lens 32 for adjusting the monitor light emitted from the light source 30 to parallel light. As the light source 30, the center wavelength λ ₀ = 960, 1450, 1960, 30 at which water has a large absorbance is used.
It is preferable that the light source emits light including any part near 00 nm. For example, when light having a center wavelength λ ₀ = 1450 nm is included, the light source 30 has a wavelength of 1300 nm to 1300 nm.
It is preferable to use a light source that emits light near 600 nm. Examples of the light source 30 include a halogen lamp, a plurality of infrared LEDs (light emitting diodes), and LDs (laser diodes).

The first light receiving device 16 propagates through the sample water W.
A monitor that is carried and reflected back by the reflector 22
The lens 34 for converging light and the above-described principle
As described at the time, the differential absorbance of the sample water W is the highest.
Large wavelength λ₁Bandpass filter that transmits only
Filter 36 and the light transmitted through the bandpass filter 36.
A photoda that illuminates and converts the light intensity into an analog electrical signal
And a light receiving element 38 such as an ion. This embodiment
Then, the band-pass filter 36 has a predetermined wavelength λ. ₁,example
For example, it has a function of transmitting only light having a wavelength of 1420 nm.

The second light receiving device 18 propagates through the sample water W.
A monitor that is carried and reflected back by the reflector 22
The lens 40 for converging light and the above-described principle
As described at the time, the differential absorbance of the sample water W is the highest.
Small wavelength λ_TwoBandpass filter that transmits only
Filter 42 and the light transmitted through the bandpass filter 42.
A photoda that illuminates and converts the light intensity into an analog electrical signal
And a light receiving element 44 such as an ion. This embodiment
Then, the band-pass filter 42 has a predetermined wavelength λ. _Two,example
For example, it has a function of transmitting only light of 1480 nm.

The microscope device 20 reflects the monitor light emitted from the light source device 14 toward the sample water W, and transmits the monitor light that propagates in the sample water W, is reflected by the reflection plate 22, and returns. And a part of the monitor light transmitted through the half mirror 46 is reflected and received by the second light receiving device 18.
It has a half mirror 50 for transmitting the remaining light to the first light receiving device 16 to receive light, and an objective lens 48 for obtaining spatial resolution.

The evaluation device 10 includes an A / D converter 5
2, an arithmetic and control unit 54, and a display unit 55 for displaying the result calculated by the arithmetic and control unit 54. The A / D converter 52 includes the first and second light receiving devices 1
6 and 18, and has a function of converting analog electric signals sent from the first and second light receiving devices 16 and 18 into digital electric signals.

The arithmetic and control unit 54 includes an A / D converter 5
2, and based on a digital electric signal sent from the A / D converter 52, the difference absorbance at the wavelength λ ₁ between the state where the magnetic field is applied to the sample water W and the state where the magnetic field is not applied to the sample water W. , The difference absorbance at the wavelength λ ₂ , and further, the maximum amplitude ΔA of the difference absorbance =
A ₁ + | A ₂ | is calculated. The arithmetic and control unit 54 is also electrically connected to the constant temperature control circuit 56, the magnetic field application control circuit 58, and the stage control circuit 60 described above.
It also has a function to control these.

The display device 55 is, for example, a CRT or a printing device, and is electrically connected to the arithmetic and control unit 54. The display unit 55 displays the value of the maximum amplitude ΔA of the differential absorbance for each measurement location calculated by the arithmetic and control unit 54. For example, using shades of color 2
Display dimensionally.

Next, an example of the water motility evaluation method according to the present embodiment using the evaluation apparatus 10 having the above-described configuration will be described with reference to the flowchart of FIG.

First, the sample water W is placed in the sample container 11.
After being filled in the constant temperature chamber 12, the temperature of the sample water W is maintained at a predetermined temperature by the constant temperature control circuit 56 (step S101). As the sample water W, the sample container 11 may be simply filled with natural water to evaluate its motility, or a lotion or the like may be filled to evaluate the emulsion. Furthermore, it is also possible to put the food in the sample container 11 and evaluate the motility of water in the food, or put the skin of a living body and evaluate the motility of water in the skin.

Next, a range to be measured for the sample water W is designated and input to the arithmetic and control means 54 (step S1).
02).

Next, the magnetic field application device 28 is stopped by the magnetic field application control circuit 58, so that no magnetic field is applied to the sample water W (step S103). Thereafter, the light source device 14 emits monitor light. The monitor light emitted from the light source device 14 is reflected by the half mirror 46 and irradiates the sample water W in the sample container 11 via the objective lens 48.

The monitor light propagating in the sample water W is
The light is reflected by the reflection plate 22 and reaches the half mirror 50 through the objective lens 48 and the half mirror 46. A part of the monitor light reaching the half mirror 50 is
And is received by the first light receiving device 16. On the other hand, another part of the monitor light reaching the half mirror 50 is reflected by the half mirror 50 and received by the second light receiving device 18.

In the monitor light received by the first light receiving device 16, only light having a predetermined wavelength λ ₁ , for example, 1420 nm, is transmitted by the band-pass filter 36, received by the light receiving element 38, and the light intensity is converted into analog electric light. Converted to a signal. On the other hand, the monitor light received by the second light receiving device 18 has a predetermined wavelength λ ₂ ,
For example, only light of 1480 nm is transmitted and the light receiving element 44
And the light intensity is converted into an analog electric signal. The analog electric signals from the light receiving elements 38 and 44 are sent to the A / D converter 52, where they are converted into digital electric signals. Then, the digital electric signal converted by the A / D converter 52 is sent to the arithmetic and control unit 54 and stored in association with the measurement position (step S104).

Next, the magnetic field application device 28 is driven by the magnetic field application control circuit 58 to apply a magnetic field to the sample water W (step S105). At this time, the intensity of the magnetic field applied to the sample water W is 200 mT (2000 gauss) or more,
Preferably, it is 400 mT to 2000 mT. When the intensity of the applied magnetic field is smaller than 200 mT,
There is a tendency that the measurement accuracy of the maximum amplitude ΔA of the measured differential absorbance, and consequently, the accuracy of evaluating the motility of the sample water W is reduced. The direction of the applied magnetic field is preferably on the optical axis of the monitor light.

In a state where a magnetic field is applied to the sample water W, the monitor light received by the first light receiving device 16 has a predetermined wavelength λ ₁ , for example, 1420 n, by the band-pass filter 36.
Only the light of m is transmitted, received by the light receiving element 38, and its light intensity is converted into an analog electric signal. on the other hand,
The monitor light received by the second light receiving device 18 has a predetermined wavelength λ ₂ , for example, 1480, by the bandpass filter 42.
Only the light of nm is transmitted, received by the light receiving element 44, and the light intensity is converted into an analog electric signal. Analog electric signals from the light receiving elements 38 and 44 are A /
The signal is sent to a D converter 52, where it is converted into a digital electric signal. Then, the digital electric signal converted by the A / D converter 52 is sent to the arithmetic and control unit 54 and stored in association with the measurement position (step S106).

Next, the wavelength λ is measured with the magnetic field applied to the sample water W and stored in the arithmetic and control unit 54.
The data of the light intensity of ₁ and λ ₂ and the wavelength λ ₁ measured without applying a magnetic field and stored in the arithmetic and control unit 54
And the light intensity data of λ ₂ , the arithmetic and control unit 54 calculates the differential absorbances A ₁ and A ₂ of the sample water W at the wavelengths λ ₁ and λ ₂ , and furthermore, the maximum amplitude ΔA = A _{1 of the} differential absorbance. + | A ₂ | is calculated. Then, the value of the calculated maximum amplitude ΔA of the differential absorbance is stored in association with the measured position (step S107).

Next, the electric stage 26 is driven by the stage control circuit 60 to change the field of view (step S).
108). Then, it is determined whether or not the changed position is within the measurement range (step S109). If the changed position is within the measurement range, the process returns to step S103 to repeat the above measurement.

The calculation of the maximum amplitude ΔA of the differential absorbance is completed for all the measurement ranges, and the field of view is changed by the electric stage 26 in step S108, and step S10 is performed.
If it is determined in step 9 that the position is out of the measurement range, the process proceeds to step S110.

In step S110, the arithmetic control means 54
The value of the maximum amplitude ΔA of the differential absorbance at each measurement position is two-dimensionally displayed on the display device 55 using, for example, the density of the color based on the data stored in the display device 55. This makes it possible to evaluate the motility of the water at each position within the measurement range of the sample water W.

Here, in this embodiment, the evaluation of water motility may be performed as follows. Another example of the evaluation method according to the present embodiment will be described with reference to the flowchart in FIG.

First, the sample water W is placed in the sample container 11.
After being filled in the constant temperature chamber 12, the temperature of the sample water W is maintained at a predetermined temperature by the constant temperature control circuit 56 (step S201). Next, a range to be measured for the sample water W is specified and input to the arithmetic and control unit 54 (step S202).

Next, the magnetic field application device 28 is stopped by the magnetic field application control circuit 58, so that no magnetic field is applied to the sample water W (step S203). Thereafter, the light source device 14 emits monitor light. The monitor light emitted from the light source device 14 is reflected by the half mirror 46 and irradiates the sample water W in the sample container 11 via the objective lens 48.

The monitor light propagating in the sample water W is
The light is reflected by the reflection plate 22 and reaches the half mirror 50 through the objective lens 48 and the half mirror 46. A part of the monitor light reaching the half mirror 50 is
And is received by the first light receiving device 16. On the other hand, another part of the monitor light reaching the half mirror 50 is reflected by the half mirror 50 and received by the second light receiving device 18.

In the monitor light received by the first light receiving device 16, only light having a predetermined wavelength λ ₁ , for example, 1420 nm, is transmitted by the band-pass filter 36, and is received by the light receiving element 38, and the light intensity is analog. Converted to a signal. On the other hand, the monitor light received by the second light receiving device 18 has a predetermined wavelength λ ₂ ,
For example, only light of 1480 nm is transmitted and the light receiving element 44
And the light intensity is converted into an analog electric signal. The analog electric signals from the light receiving elements 38 and 44 are sent to the A / D converter 52, where they are converted into digital electric signals. Then, the digital electric signal converted by the A / D converter 52 is sent to the arithmetic and control unit 54 and stored in association with the measurement position (step S204).

Next, the electric stage 26 is driven by the stage control circuit 60 to change the field of view (step S).
205). Then, it is determined whether or not the changed position is within the measurement range (step S206). If the changed position is within the measurement range, the process returns to step S203 to repeat the above-described measurement.

In the state where no magnetic field is applied to the sample water W, the light intensities of the wavelengths λ ₁ and λ ₂ are measured for all the measurement ranges, and after the data storage is completed, the step S 2
In step 05, the visual field is changed by the electric stage 26, and when it is determined in step S206 that the position is outside the measurement range, the process proceeds to step S207.

In step S207, the magnetic field application device 28 is driven by the magnetic field application control circuit 58, and the sample water W
To a state where a magnetic field is applied. At this time, the intensity of the magnetic field applied to the sample water W is preferably 200 mT (2000 gauss) or more, preferably 400 mT to 2000 mT. If the intensity of the applied magnetic field is smaller than 200 mT, the measurement accuracy of the maximum amplitude ΔA of the measured differential absorbance, and thus the accuracy of the evaluation of the motility of the sample water W, tend to decrease. The direction of the applied magnetic field is preferably on the optical axis of the monitor light.

In a state where a magnetic field is applied to the sample water W, the monitor light received by the first light receiving device 16 is subjected to a predetermined wavelength λ ₁ , for example, 1420 n by the band-pass filter 36.
Only the light of m is transmitted, received by the light receiving element 38, and its light intensity is converted into an analog electric signal. on the other hand,
The monitor light received by the second light receiving device 18 has a predetermined wavelength λ ₂ , for example, 1480, by the bandpass filter 42.
Only the light of nm is transmitted, received by the light receiving element 44, and the light intensity is converted into an analog electric signal. Analog electric signals from the light receiving elements 38 and 44 are A /
The signal is sent to a D converter 52, where it is converted into a digital electric signal. Then, the digital electric signal converted by the A / D converter 52 is sent to the arithmetic and control unit 54 and stored in association with the measurement position (step S208).

Next, the electric stage 26 is driven by the stage control circuit 60 to change the field of view (step S).
209). Then, it is determined whether or not the changed position is within the measurement range (step S210), and if it is within the measurement range, the process returns to step S208 to repeat the above measurement.

In a state where a magnetic field is applied to the sample water W, the light intensities of the wavelengths λ ₁ and λ ₂ are measured in all the measurement ranges, and after the data storage is completed, the process proceeds to step S 20.
In step 9, the field of view is changed by the motorized stage 26. If it is determined in step S210 that the position is outside the measurement range, the process proceeds to step S211.

In step S211, the measurement is performed with the magnetic field applied to the sample water W, the data of the light intensity of the wavelengths λ ₁ and λ ₂ stored in the arithmetic and control unit 54 and the measurement without the magnetic field applied. Based on the light intensity data of the wavelengths λ ₁ and λ ₂ stored in the arithmetic and control unit 54, the arithmetic and control unit 54 calculates the differential absorbances A ₁ and A ₂ of the sample water W at the wavelengths λ ₁ and λ ₂ . Calculated from this, the maximum amplitude ΔA of the differential absorbance for all measurement positions
= A ₁ + | A ₂ |

Then, the value of the maximum amplitude ΔA of the differential absorbance for each measurement position calculated by the arithmetic and control means 54 is displayed two-dimensionally on the display device 55 using, for example, the density of the color (step S212). This makes it possible to evaluate the motility of the water at each position within the measurement range of the sample water W.

The two evaluation methods have been described above with reference to the flowcharts of FIGS. The evaluation method described with reference to the flowchart of FIG. 5 uses a technique of performing a magnetic field ON / OFF operation each time for one measurement position and measuring a differential absorbance. In this method, it is possible to measure the differential absorbance without moving the measurement position at a certain measurement point. Therefore, although the measurement accuracy is high, it is necessary to turn on / off the magnetic field for each measurement position. The measurement time becomes longer by the minute.

On the other hand, the evaluation method described with reference to the flowchart of FIG.
Although the measurement time can be shortened, the measurement time can be shortened, but the measurement position shifts due to the problem of the position reproducibility of the electric stage 26, and an error may occur in the maximum amplitude ΔA of the measured differential absorbance.

Therefore, it is preferable to use the above-described two evaluation methods in consideration of the state of the sample water W and the required evaluation accuracy.

As described above, in the present embodiment, the mobility of the sample water W is evaluated based on the difference in absorbance caused by applying a magnetic field to the sample water W. As described above, since the absorbance of the sample water W is measured using light, NMR
As a result, the analysis work is simplified as compared with the case described above, and a measurement with a high spatial resolution can be performed in a short time, so that the efficiency of evaluation is improved.

Since light is used as a measurement probe, other sample information can be simultaneously measured with light. On the other hand, in the evaluation method using NMR, it is difficult to optically measure other sample information at the same time due to a problem such as an apparatus configuration.
And the evaluation device is excellent.

(Second Embodiment) Hereinafter, a second embodiment of the water motility evaluation method and evaluation apparatus according to the present invention will be described. The same elements as those described in the first embodiment are denoted by the same reference numerals, and redundant description will be omitted.

First, before describing the evaluation method and the evaluation apparatus according to the second embodiment, the principle of evaluating the motility of water in the present embodiment will be described with reference to FIGS.

The Faraday rotation (porcelain rotation effect) is based on the fact that a linearly polarized electromagnetic wave propagates through a dielectric medium along a magnetic field.
This is a phenomenon in which the plane of polarization rotates. Water is also known as a Faraday rotating substance. In the optical phenomenon, FIG.
As shown in (2), when linearly polarized light passes through the sample water W to which the magnetic field is applied, the deflection angle changes. This change in the deflection angle (Faraday rotation angle θ) can be obtained as the difference between the deflection angle when the magnetic field is applied and the deflection angle when the magnetic field is not applied. However, assuming that the deflection angle in a state where no magnetic field is applied is a reference (deflection angle = 0 degree), this change in the deflection angle (Faraday rotation angle θ) can be represented by the deflection angle in a state where a magnetic field is applied. In the evaluation method described in the present embodiment, the deflection angle in a state where a magnetic field is applied is referred to as a Faraday rotation angle θ, which is based on the deflection angle in a state where no magnetic field is applied (deflection angle = 0 degrees). Yes,
Even in this case, the Faraday rotation angle θ is obtained as the difference between the deflection angle when the magnetic field is applied and the deflection angle when the magnetic field is not applied. Therefore, the evaluation method according to the present embodiment for obtaining the Faraday rotation angle θ based on the deflection angle in a state where a magnetic field is applied,
This is included in the concept of the present invention of "evaluating the mobility of sample water based on a difference between a deflection angle measured in a state where a magnetic field is applied and a deflection angle in a state where no magnetic field is applied".

Here, the gel has the property that the higher the concentration, the less water (free water) with high mobility and the more water (bound water) with low mobility. Therefore, the inventor prepared a plurality of samples having different gel concentrations and examined the relationship between the gel concentration and the Faraday rotation angle θ. Then, FIG.
As shown in (2), the Faraday rotation angle θ tended to decrease as the gel concentration increased. As described above, the inventors have found that there is a correlation between the gel concentration and the Faraday rotation angle θ, and it is possible to evaluate the motility of water by measuring the Faraday rotation angle θ. For example, θ
Is large when the gel concentration is low, and it can be evaluated that the water motility is high (there is a lot of free water). On the other hand, when θ is small, it corresponds to a high gel concentration, and it can be evaluated that the mobility of water is low (the amount of bound water is large).

An evaluation method and an evaluation apparatus according to the present embodiment described below are based on the above-described principle.

FIG. 9 is a diagram schematically showing the configuration of the evaluation device according to the present embodiment. As shown in the figure, the evaluation device 10 includes a constant temperature chamber 12 containing sample water (sample water) W.
A light source device 14 for irradiating the sample water W with monitor light, and a deflection angle detection device 62 for receiving the monitor light propagated in the sample water W and detecting its deflection angle.
And a microscope device 6 for obtaining a spatial resolution at a cell level
4 is provided.

The constant temperature chamber 12 has the same configuration as the constant temperature chamber included in the evaluation device according to the first embodiment. Then, similarly to the evaluation device according to the first embodiment, the constant temperature chamber 12 is electrically connected to the constant temperature control circuit 56, and the magnetic field application device 28 is electrically connected to the magnetic field application control circuit 58. And electric stage 2
6 is electrically connected to the stage control circuit 60.

The light source device 14 has the same configuration as the light source device included in the evaluation device according to the above-described first embodiment. However, in this embodiment, the light source 30 is composed of a laser that emits linearly-polarized monitor light. I have.

The deflection angle detecting device 62 receives the monitor light converged by the lens 34 for converging the monitor light propagating in the sample water W and reflected by the reflector 22 and returning. And a deflection angle detector 38 for detecting the deflection angle. In the present embodiment, the deflection angle measured with the magnetic field applied is set as the Faraday rotation angle θ based on the deflection angle of the monitor light with no magnetic field applied.
Is based on the deflection angle of the monitor light detected by the deflection angle detection device, for calculating the difference between the deflection angle of the monitor light propagating through the sample water in a state where a magnetic field is applied to the sample water and in a state where no magnetic field is applied to the sample water. Computing device ". However, when the deflection angle of the monitor light in a state where no magnetic field is applied is not used as a reference, the deflection angle measured in a state where a magnetic field is applied by the arithmetic and control unit 54 described later is different from the deflection angle in a state where no magnetic field is applied. Faraday rotation angle θ
In this case, the arithmetic and control unit 54 functions as the “arithmetic unit”.

The microscope device 64 reflects the monitor light emitted from the light source device 14 toward the sample water W, and transmits the monitor light that propagates in the sample water W, is reflected by the reflection plate 22, and returns. And an objective lens 48 for obtaining a spatial resolution.

The evaluation device 10 includes an arithmetic control device 54
And a display device 55 for displaying the result calculated by the arithmetic and control unit 54.

The arithmetic and control unit 54 is electrically connected to the deflection angle detector 66, performs various calculations, and stores the Faraday rotation angle data sent from the deflection angle detector 62. . The arithmetic and control unit 54 is also electrically connected to the constant temperature control circuit 56, the magnetic field application control circuit 58, and the stage control circuit 60, and has a function of controlling these.

The display device 55 is, for example, a CRT or a printing device, and is electrically connected to the arithmetic and control unit 54. The display unit 55 displays the Faraday rotation angle θ for each measurement location stored in the arithmetic and control unit 54, for example, for the color. Display two-dimensionally using shading.

Next, an example of the water motility evaluation method according to the present embodiment using the evaluation apparatus 10 having the above-described configuration will be described with reference to the flowchart of FIG.

First, the objective lens 48 and the sample container 1
The Faraday rotation angle θ0 in a state where the sample water W is not present is measured in order to cause a slight Faraday rotation of the cover glass 24 and the like constituting 1. First, the sample container 11 not filled with the sample water W is accommodated in the constant temperature chamber 12, and the temperature of the sample container 11 is maintained at a predetermined temperature by the constant temperature control circuit 56. Next, the magnetic field application device 28 is driven by the magnetic field application control circuit 58 to apply a magnetic field to the sample container 11 (step S301). At this time, the intensity of the magnetic field applied to the sample container 11 is 200 m
T (2000 gauss) or more, preferably 400 mT to 2
000 mT is preferable. The strength of the applied magnetic field is 2
If it is smaller than 00 mT, the measurement accuracy of the measured Faraday rotation angle θ0 and, consequently, the accuracy of the evaluation of the mobility of the sample water W tend to decrease. The direction of the applied magnetic field is preferably on the optical axis of the monitor light.

The light source device 14 emits linearly polarized monitor light. The monitor light emitted from the light source device 14 is reflected by the half mirror 46 and
The sample container 11 is irradiated with the sample through the sample container 8. The monitor light that has propagated in the sample container 11 is reflected by the reflection plate 22, and reaches the deflection angle detection device 62 through the objective lens 48 and the half mirror 46. The monitor light reaching the deflection angle detection device 62 is converged by the lens 34 and received by the deflection angle detector 66 to detect the deflection angle θ0. The detected deflection angle θ0 is sent to the arithmetic and control unit 54 and stored (step S302).

Next, the sample water W is placed in the sample container 11.
After being filled in the constant temperature chamber 12, the temperature of the sample water W is maintained at a predetermined temperature by the constant temperature control circuit 56 (step S303). And the arithmetic control means 54
The user designates and inputs a measurement range of the sample water W (step S304).

Then, in the state where a magnetic field is applied to the sample water W by the magnetic field applying device 28, linearly polarized monitor light is emitted from the light source device 14. The monitor light emitted from the light source device 14 is reflected by the half mirror 46 and passes through the objective lens 48 into the sample water W in the sample container 11.
Is irradiated.

The monitor light propagating in the sample water W is
The light is reflected by the reflection plate 22 and reaches the deflection angle detection device 62 through the objective lens 48 and the half mirror 46. The monitor light reaching the deflection angle detection device 62 is converged by the lens 34, received by the deflection angle detector 66, and the deflection angle θ1 is detected (step S305). Detected deflection angle θ1
Is sent to the arithmetic and control means 54, and based on the deflection angle θ0 and the deflection angle θ1 without the sample water W stored earlier, the Faraday rotation angle θ =
θ1−θ0 is calculated. Then, the calculated Faraday rotation angle θ based on only the sample water W is stored as data in association with the measurement position (step S306).

Next, the electric stage 26 is driven by the stage control circuit 60 to change the field of view (step S).
307). Then, it is determined whether or not the changed position is within the measurement range (step S308). If the changed position is within the measurement range, the process returns to step S305 to repeat the above measurement.

The Faraday rotation angle θ is calculated for all the measurement ranges, the field of view is changed by the electric stage 26 in step S307, and if the position is determined to be out of the measurement range in step S308, the process proceeds to step S30.
Move to 9.

In step S309, the arithmetic control means 54
The two-dimensional display of the value of the Faraday rotation angle θ for each measurement position on the display device 55 using, for example, color shading based on the data stored in the display device 55. Finally, the application of the magnetic field to the sample water W is stopped by the magnetic field application control circuit 28 (step S310).

Thus, it is possible to evaluate the motility of water at each position within the measurement range of the sample water W.

As described above, in the present embodiment, the mobility of the sample water W is evaluated based on the Faraday rotation angle generated by applying a magnetic field to the sample water W. As described above, since the Faraday rotation angle of the sample water W is measured using light, the analysis work is simplified as compared with the case using NMR, and the measurement with high spatial resolution can be performed in a short time. Thus, the efficiency of the evaluation is improved.

In the absence of the sample water W, the deflection angle θ
0, and the polarization angle θ0 is subtracted from the polarization angle θ1 measured in a state where the sample lens W is filled.
Since the influence of the Faraday rotation which may be caused by the cover glass 24 constituting the sample container 8 or the sample container 11 is corrected, the accuracy of the evaluation is improved.

Also, in this embodiment, since light is used as a measurement probe, other sample information can be simultaneously measured with light. On the other hand, in the evaluation method using NMR, it is difficult to optically measure other sample information at the same time due to problems such as the device configuration, and the evaluation method and the evaluation device according to the present embodiment are also excellent in this regard. .

(Third Embodiment) Hereinafter, a third embodiment of the water motility evaluation method and evaluation apparatus according to the present invention will be described. The same elements as those described in the first and second embodiments are denoted by the same reference numerals, and redundant description will be omitted.

First, before describing the evaluation method and the evaluation apparatus according to the third embodiment, the principle of evaluating the motility of water in the present embodiment will be described with reference to FIGS. 11 and 12.

The principle of evaluating the mobility of water in this embodiment is similar to the principle of evaluating the mobility of water in the second embodiment, in which linearly polarized light passes through sample water W to which a magnetic field is applied. In doing so, the principle of Faraday rotation in which the deflection angle changes is used. However, since it is difficult to measure the Faraday rotation angle in a short time, the present embodiment estimates the Faraday rotation angle by using a simple method.

When the linearly polarized monitor light irradiating the sample water W is scattered, the monitor light has broad light characteristics with respect to the deflection angle as shown by the solid line L1 in FIG. At this time, the light having the minimum light intensity P, that is, light having a deflection angle of 90 degrees with respect to the linearly polarized light is received, and the light intensity P0 is measured.

Next, when a magnetic field is applied to the sample water W, the distribution of the light intensity P with respect to the deflection angle shifts due to the Faraday rotation as shown by the solid line L2. even here,
Light having a deflection angle of 90 degrees is received, and its light intensity P1 is measured.

Then, the ratio is calculated based on P1 and P0, and as shown in FIG. 12, the Faraday rotation angle is calculated based on the relationship between the previously measured Faraday rotation angle θ and the ratio P1 / P0. The value of θ can be estimated.

An evaluation method and an evaluation device according to the present embodiment described below are based on the above principle.

FIG. 13 is a diagram schematically showing the configuration of the evaluation device according to the present embodiment. As shown, the evaluation device 10
Is a constant temperature chamber 1 for storing sample water (sample water) W
2, a light source device 14 for irradiating the sample water W with monitor light, a light receiving device 70 for receiving monitor light propagated through the sample water W and receiving only light having a predetermined deflection angle, And a microscope device 64 for obtaining a spatial resolution of

The constant temperature chamber 12 has basically the same configuration as the constant temperature chamber provided in the evaluation apparatus according to the first and second embodiments. However, in this embodiment, a monitor is provided in the cover glass 24 of the sample container 11. A scatterer 25 such as fine particles for scattering light is added. Further, similarly to the evaluation devices according to the first and second embodiments, the constant temperature chamber 12 is electrically connected to the constant temperature control circuit 56, and the magnetic field application device 28 is connected to the magnetic field application control circuit 58.
The motorized stage 26 is electrically connected to the stage control circuit 60.

The light source device 14 has the same configuration as the light source device included in the evaluation device according to the above-described second embodiment, and the light source 30 is composed of a laser or the like that emits linearly polarized monitor light.

The light receiving device 70 is provided with a lens 34 for converging the monitor light propagating in the sample water W, reflected by the reflector 22 and returning, and a predetermined deflection of the monitor light converged by the lens 34. A polarizing element 72 that transmits only light having an angle (90 degrees in the present embodiment);
Light receiving element 7 for receiving light transmitted through polarizing element 72
And 4.

The microscope device 64 reflects the monitor light emitted from the light source device 14 toward the sample water W, and transmits the monitor light that propagates in the sample water W, is reflected by the reflection plate 22, and returns. And an objective lens 48 for obtaining a spatial resolution.

The evaluation device 10 includes an A / D converter 5
2, an arithmetic and control unit 54, and a display unit 55 for displaying the result calculated by the arithmetic and control unit 54. The A / D converter 52 is electrically connected to the light receiving element 74 and has a function of converting an analog electric signal sent from the light receiving element 74 into a digital electric signal.

The arithmetic and control unit 54 includes the A / D converter 5
2, and has a deflection angle of 90 degrees between a state in which a magnetic field is applied to the sample water W and a state in which no magnetic field is applied to the sample water W based on a digital electric signal sent from the A / D converter 52. The light intensity ratio P1 / P0 is calculated, and the Faraday rotation angle θ is calculated based on the relationship between the light intensity ratio P1 / P0 stored in advance and the Faraday rotation angle θ.
Is estimated. The arithmetic and control unit 54 is also electrically connected to the constant temperature control circuit 56, the magnetic field application control circuit 58, and the stage control circuit 60, and has a function of controlling these.

The display device 55 is, for example, a CRT or a printing device, and is electrically connected to the arithmetic and control unit 54. The display unit 55 displays the value of the Faraday rotation angle θ for each measurement location estimated by the arithmetic and control unit 54, for example, in color. Is displayed two-dimensionally using the shading of.

Next, an example of the water mobility evaluation method according to the present embodiment using the evaluation device 10 having the above-described configuration will be described with reference to the flowchart of FIG.

First, the angle of the deflection element 72 is adjusted (step S401). At this time, when the scattered monitor light is received by the light receiving element 74, the angle at which the light intensity becomes minimum, that is, the angle formed by the polarization plane of the linearly polarized monitor light emitted from the light source 30 becomes 90 degrees. Adjust that angle.

Next, the sample water W is placed in the sample container 11.
Is filled and stored in the constant temperature chamber 12, and then the temperature of the sample water W is maintained at a predetermined temperature by the constant temperature control circuit 56 (step S402). And the arithmetic control means 54
The user designates and inputs a measurement range of the sample water W (step S403).

Next, the magnetic field application device 28 is stopped by the magnetic field application control circuit 58, so that no magnetic field is applied to the sample water W (step S404). Thereafter, the light source device 14 emits linearly polarized monitor light. The monitor light emitted from the light source device 14 is supplied to the half mirror 46.
And irradiates the sample water W in the sample container 11 via the objective lens 48. At this time, the monitor light is scattered by the scatterer 25 added to the cover glass 24 of the sample container 11.

The monitor light propagating in the sample water W is
The light is reflected by the reflection plate 22, propagates again in the sample water W, and is scattered by the scatterer 25 added to the cover glass 24. Then, the objective lens 48 and the half mirror 4
6 to the light receiving device 70. The monitor light reaching the light receiving device 70 is converged by the lens 34 and is
2, only light having a deflection angle of 90 degrees is transmitted. The light transmitted through the polarizing element 72 is received by the light receiving element 74,
The light intensity P0 is converted into an analog electric signal. The analog electric signal from the light receiving element 74 is sent to the A / D converter 52, where it is converted into a digital electric signal. Then, the digital electric signal converted by the A / D converter 52 is sent to the arithmetic and control unit 54, where the data is stored (step S405).

Next, the magnetic field application device 28 is driven by the magnetic field application control circuit 58 so that a magnetic field is applied to the sample water W (step S406). At this time, the intensity of the magnetic field applied to the sample water W is preferably 200 mT (2000 gauss) or more, preferably 400 mT to 2000 mT. When the intensity of the applied magnetic field is smaller than 200 mT, the measurement accuracy of the measured light intensity ratio P1 / P0,
Eventually, the accuracy of the evaluation of the mobility of the sample water W tends to decrease. The direction of the applied magnetic field is preferably on the optical axis of the monitor light.

In the state where a magnetic field is applied to the sample water W, the monitor light received by the light receiving device 70 is converged by the lens 34, and only light having a deflection angle of 90 degrees is transmitted by the polarizing element 72. The light transmitted through the polarizing element 72 is received by the light receiving element 74, and the light intensity P1 is converted into an analog electric signal. The analog electric signal from the light receiving element 74 is sent to the A / D converter 52, where it is converted into a digital electric signal. Then, the digital electric signal converted by the A / D converter 52 is sent to the arithmetic and control unit 54 and stored therein (step S40).
7).

Next, in the arithmetic and control unit 54, based on the stored light intensities P0 and P1, the light intensity ratio P1
/ P0 is calculated, and the light intensity ratio P1 / stored in advance is calculated.
The Faraday rotation angle θ is estimated based on the relationship between P0 and the Faraday rotation angle θ. Then, the estimated Faraday rotation angle θ is stored in association with the measurement position (step S408).

Next, the electric stage 26 is driven by the stage control circuit 60 to change the field of view (step S).
409). Then, it is determined whether or not the changed position is within the measurement range (step S410). If the changed position is within the measurement range, the process returns to step S404 to repeat the above-described measurement.

The Faraday rotation angle θ is estimated for all the measurement ranges, the visual field is changed by the electric stage 26 in step S409, and if the position is determined to be out of the measurement range in step S410, step S41 is performed.
Move to 1.

In step S411, the arithmetic control means 54
Is displayed two-dimensionally on the display device 55 using, for example, color shading based on the data stored in the Faraday rotation angle θ for each measurement position. This makes it possible to evaluate the motility of the water at each position within the measurement range of the sample water W.

As described above, in the present embodiment, the mobility of the sample water W is evaluated based on the Faraday rotation angle generated by applying a magnetic field to the sample water W. As described above, since the Faraday rotation angle of the sample water W is measured using light, the analysis work is simplified as compared with the case using NMR, and the measurement with high spatial resolution can be performed in a short time. Thus, the efficiency of the evaluation is improved. In particular, in a state where a magnetic field is applied to the sample water W and in a state where no magnetic field is applied, the monitor light is scattered by the scatterer 25, and the intensity of light having a predetermined deflection angle is received by the polarizing element 72. Since the Faraday rotation angle is estimated on the basis of the above, the calculation of the Faraday rotation angle is easier than in the second embodiment, and the efficiency of the evaluation is further improved.

Also, in this embodiment, since light is used as a measurement probe, other sample information can be simultaneously measured with light. On the other hand, in the evaluation method using NMR, it is difficult to optically measure other sample information at the same time due to problems such as the device configuration, and the evaluation method and the evaluation device according to the present embodiment are also excellent in this regard. .

Note that the present invention is not limited to the above-described embodiment, and various modifications can be made.

For example, in the above-described third embodiment, the magnetic field is turned on / off every time at one measurement position.
By performing this for all measurement positions, the light intensity ratio P
Although the method of calculating 1 / P0 was used, the present invention is not limited to this.
In the same manner as described with reference to the flowchart of FIG.
0, then the light intensity P1 is measured at all the measurement positions while the magnetic field is turned on, and the light intensity ratio P is measured at all the measurement positions based on these light intensities P0 and P1.
1 / P0 may be obtained.

[0118]

According to the present invention, it is possible to simplify the evaluation work and evaluate the efficiency of the evaluation of water motility.

[Brief description of the drawings]

FIG. 1 is a graph showing how an absorbance spectrum shifts when a magnetic field is applied to sample water.

FIG. 2 is a graph showing a differential absorbance spectrum when a magnetic field is applied to a sample water and when no magnetic field is applied.

FIG. 3 is a graph showing the relationship between the gel concentration and the maximum amplitude ΔA of the differential absorbance.

FIG. 4 is a diagram schematically illustrating a configuration of a water mobility evaluation apparatus according to the first embodiment.

FIG. 5 is a flowchart for explaining an example of a water motility evaluation method according to the first embodiment.

FIG. 6 is a flowchart for explaining another example of the water mobility evaluation method according to the first embodiment.

FIG. 7 is a diagram schematically showing a state in which linearly polarized light passes through sample water and changes the deflection angle.

FIG. 8 is a graph showing the relationship between gel concentration and Faraday rotation angle θ.

FIG. 9 is a diagram schematically illustrating a configuration of a water mobility evaluation apparatus according to a second embodiment.

FIG. 10 is a flowchart illustrating an example of a water motility evaluation method according to the second embodiment.

FIG. 11 is a graph showing how the distribution of light intensity with respect to the deflection angle of light of linearly scattered light is shifted by applying a magnetic field to sample water.

FIG. 12 is a graph showing a relationship between a Faraday rotation angle θ and a light intensity ratio P1 / P0.

FIG. 13 is a diagram schematically illustrating a configuration of a water mobility evaluation apparatus according to a third embodiment.

FIG. 14 is a flowchart illustrating an example of a water motility evaluation method according to the third embodiment.

[Explanation of symbols]

10 ... water motility evaluation device, 11 ... sample container, 12
... constant temperature chamber, 14 ... light source device, 16, 18 ... light receiving device, 20, 64 ... microscope device, 25 ... scatterer, 26 ... electric stage, 28 ... magnetic field applying device, 36, 42 ... band pass filter, 38, 44 ... light receiving element, 54 ... arithmetic and control unit, 62 ... deflection angle detecting apparatus, 72 ... polarizing element, 74
... light receiving element, W ... sample water.

Claims (10)

[Claims] 1. In a state where a magnetic field is applied to sample water,
Irradiating the sample water with monitor light and receiving the monitor light propagated through the sample water, measuring predetermined spectroscopic properties of the sample water based on the received monitor light, The mobility of the sample water is evaluated based on the predetermined spectroscopic properties of the sample water measured in a state where the magnetic field is applied and the predetermined spectroscopic properties of the sample water in a state where the magnetic field is not applied. A method for evaluating water motility, comprising: 2. The absorbance at a predetermined wavelength as the predetermined spectroscopic property of the sample water is measured. The motility evaluation method for water according to claim 1, wherein the motility of the sample water is evaluated based on the motility. 3. A state in which linearly polarized light is used as the monitor light, a deflection angle of the monitor light propagating through the sample water as the predetermined spectroscopic property of the sample water is measured, and the magnetic field is applied. 2. The water mobility evaluation method according to claim 1, wherein the mobility of the sample water is evaluated based on a difference between the deflection angle measured in the step (a) and the deflection angle when the magnetic field is not applied. . 4. Using linearly-polarized light as the monitor light, and scattering at least one of the monitor light before irradiating the sample water and the monitor light after propagating through the sample water, The intensity of light having a predetermined deflection angle of the monitor light scattered as the predetermined spectroscopic property of the sample water is measured, and the intensity of light having the predetermined deflection angle measured in a state where the magnetic field is applied is measured. The motility of water according to claim 1, wherein the motility of the sample water is evaluated based on a ratio between the intensity and the intensity of the light having the predetermined deflection angle when the magnetic field is not applied. Evaluation method. 5. The method according to claim 1, wherein the strength of the applied magnetic field is 200 mT or more. 6. A magnetic field applying device for applying a magnetic field to the sample water, a light source device for irradiating the sample water with monitor light, and receiving the monitor light propagated in the sample water. A light receiving device for calculating, based on the monitor light received by the light receiving device, a difference in absorbance at a predetermined wavelength of the sample water between a state where a magnetic field is applied to the sample water and a state where no magnetic field is applied to the sample water And a device for evaluating water motility. 7. The light receiving device according to claim 6, wherein the light receiving device includes a filter that transmits the light of the predetermined wavelength, and a light receiving element that receives the light transmitted through the filter.
Water motility evaluation apparatus according to item 1. 8. A magnetic field applying device for applying a magnetic field to the sample water, a light source device for irradiating the sample water with linearly polarized monitor light, and the monitor light propagating through the sample water. And a deflection angle detection device for detecting the deflection angle thereof, based on the deflection angle of the monitor light detected by the deflection angle detection device, a state where a magnetic field is applied to the sample water and a state where no magnetic field is applied. And a calculating device for calculating a difference in the deflection angle of the monitor light propagated in the sample water. 9. A magnetic field applying device for applying a magnetic field to the sample water, a light source device for irradiating linearly polarized monitor light to the sample water, and the light source device before irradiating the sample water. A scatterer for scattering at least one of the monitor light and the monitor light after propagating in the sample water; and a predetermined deflection angle of the monitor light propagated in the sample water scattered by the scatterer. A polarizing element that transmits only light having a light-receiving element; a light-receiving element for receiving light that has passed through the polarizing element; And a calculating device for calculating a ratio of the intensity of the light having the predetermined deflection angle to the state. 10. The water motility evaluation device according to claim 6, further comprising a positioning unit for positioning the sample water.