JP2002179587A - Indigo preparation effective for preventing/treating diabetes - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an indigo preparation and related composition effective for preventing/treating diabetes. SOLUTION: This indigo preparation is an extract from an indigo fermented product: 'Sukumo' obtained from an indigo dye manufacturing process using the leaves/stems of indigo belonging to the family Polygonaceae, or fine powder obtained by grinding the dried product of the leaves/stems and seeds of indigo by an appropriate means. This indigo preparation enables blood glucose to be controlled to a normal level as appropriate for not only patients suffered from diabetes but also those with slight diabetes, called persons with potential diabetes.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial application field) The present invention finds that, for the first time, an indigo preparation, which is a kind of Polygonaceae, and a composition thereof can be used for prevention and treatment of diabetes. Completed.

(Prior art and problems) In the treatment of diabetes, insulin sensitizers have recently been used, but their side effects have become a problem, and they have not been able to be used widely and safely. In addition, sulfonylurea agents, α-glucosidase inhibitors, and the like are widely used, but the onset of diabetes is increasing year by year, and these agents are not sufficiently fulfilling their role. It has been reported that some plant-derived components are effective for diabetes, but it cannot be said that the effects are sufficiently exhibited. An object of the present invention is to provide an indigo preparation and a composition thereof which exhibit a very clear effect in preventing and treating the onset of diabetes.

(Means for Solving the Problems) The present inventors have focused on "indigo" which is a kind of Polygonaceae plants that have been used for medicinal purposes such as antipyretic and detoxifying for a long time, and have repeatedly studied. As a result, it was confirmed that the indigo preparation and its composition markedly promote the normalization of insulin secretion and interaction with the receptor, thereby improving glucose metabolism and making it smoother. .
It has also been confirmed that this product has extremely low toxicity and can be used safely for the prevention and treatment of diabetes.

(Function) The "indigo preparation" in the present invention conjugates all processed products of the indigo plant "indigo" by physical or chemical treatment, regardless of the raw material and production method. Therefore, the indigo fermented product called "Sukumo", which is made in the process of producing indigo dye, that is, the leaves and stems of the indigo are harvested, chopped, dried, and then fermented for 2-3 months with water It is shaped like red-black humus, which is called "Sukumo" and can be used as an indigo preparation. The outline of the method for producing the indigo preparation and the composition used in the present invention is as follows.

"Indigo preparation and indigo raw material" and "Sukumo" in deep ocean water for approx. 5
% And then immersed for 2 days. This is centrifuged, and the supernatant is filtered through a Millipore filter to remove bacteria. This is referred to as "scramble solution".

After centrifuging the "Indigo preparation / indigo heating material" and the "spider solution", the supernatant is filtered through a Millipore filter to remove bacteria, and further heat-sterilized at 110 ° C for 10 minutes. This is called a "heated spider solution.""Indigo dried leaves, stems, and seed powder." The harvested indigo leaves, stems, and seeds are dried, crushed into fine powder, and then pressurized and heat sterilized. This is referred to as "indigo dried leaves / stems / seed powder".

"Indigo composition" Other plants that have been reported to be effective in preventing and treating diabetes, for example, Ciss from Brazil
us sicyoides (Insulina Insul
ina) The dried product of leaves and stems and the dried product of indigo leaves, stems and seeds are pulverized into fine powders, mixed and then sterilized under pressure and heat. This is referred to as "insulina dried leaf / stem / seed powder".

Example 1 "Indigo raw material" Indigo leaf / stem mixture cut into appropriate size
50 g of "Sukumo" fermented for 3 months after pulverization was immersed in 1 liter of deep ocean water, and then 10,000 rpm
and centrifuge for 10 minutes. Further, after filtration through a 0.2 millimicron Millipore filter, about 900 ml of a "scoop solution" is obtained. This is called "Sukumo solution"
And used for the following tests. Its solids concentration was about 1%.

Example 2 "Indigo heating material""Sukumo": A mixture of indigo leaves and stems was cut into a suitable size, powdered, and fermented for 3 months: 50
g in 1 liter of deep ocean water, then 10,000
Centrifuge at rpm for 10 minutes. The supernatant is sterilized by filtration through a 0.2 millimicron Millipore filter, and further sterilized by heating at 110 ° C. for 10 minutes. This is referred to as “heated spider solution” and used in the following tests.

Example 3 "Indigo dried leaves, stems and seed powder" The harvested indigo leaves, stems and seeds were dried and ground into a fine powder having a particle size of about 50 microns or less, and pressed and heated at 110 ° C for 10 minutes. Sterilize. This is referred to as "indigo dried leaves / stems / seed powder".

Example 4 "Insulina Dried Leaf / Stem / Seed Powder" Ci from Brazil
ssus sicoides [Insulina In
sulina] is dried to a fine powder having a particle size of about 50 μm or less, and sterilized by heating at 110 ° C. for 10 minutes. This is "Insulina Dried Leaves / Stem / Seed Powder"
Called.

(Example 5) The effect on glucose metabolism in a diabetic model animal was examined using the "splash solution" obtained in Example 1.
As a spontaneous diabetes model, 8-week-old (male) Yellow kk mice were divided into two groups, and 10 mice in each group were given a "drip solution" and deep seawater (control) arbitrarily without drinking water for 4 weeks. Oral ingestion. After glucose load of 2 g / kg of glucose was applied to each of the mice to which the “sukumo solution” was administered and the mice to which deep sea water was administered, the blood glucose level and the insulin level after 60 minutes and 120 minutes were measured. In the administered mice, a significant decrease in blood glucose level was observed (Table 1).

(Example 6) Spontaneous diabetes model mouse C57BL /
KsJ-db / db Seven-week-old females were divided into two groups and each group contained 10
"Hot spider solution (1%)" (200mg / kg)
And deep sea water were orally ingested without drinking water for 4 weeks. After glucose administration with 2 g / kg of glucose after the end of the administration, the blood glucose level and the insulin level were measured for 60 minutes and 120 minutes, respectively. As a result, a significant decrease in the blood glucose level was observed in the mouse that took the “heated spider solution”. (T
able 2).

Example 7 A clinical test was conducted on a preliminary group of patients with type 2 diabetes. The patients in these patients were on a diet and exercise regimen that had not taken any medications.
The background of the 30 patients in the reserved group is the average age 55 ± 8 years old Weight 6
7 ± 4 kg BMI: 24 ± 6 kg / m ² . The preliminary patient group was divided into two groups, and a placebo group (control) and a “heated spider solution” (3 ml) group were used, each having 15 patients. They were taken three times a day for 8 weeks each (after breakfast, 3 pm, 1 ml each before bedtime). As a result, the fasting blood sugar level and Hb
A1c was reduced (Table 3).

Example 8 The effect of suppressing postprandial hyperglycemia was confirmed as follows using the indigo dried leaf, stem, and seed powder obtained in Example 3. Seven-week-old male Wistar rats were divided into two groups, one of each of which was a non-administration control group, and the other was administered with "indigo dried leaf / stem / seed powder" (100 mg / kg) by oral gavage. 1 hour later, both groups had glucose 2 g /
The kg was loaded with glucose, and the blood glucose level and insulin level 120 minutes later were measured. A significant decrease in blood glucose level was observed in the group to which "blue-dried leaves / stems / seed powder" was administered (Tabl)
e 4).

Example 9 The effect of suppressing postprandial hyperglycemia was confirmed using "insulina dried leaves / stalks / seed powder" obtained in Example 4 as follows. Eight-week-old male Wistar rats were divided into two groups, one of which was a non-administration control group, and the other was "Insulina dried leaf / stem / seed powder" (150 mg kg).
Was administered by gavage, and 1 hour later, both groups were glucose-loaded with 2 g / kg of glucose, and the blood glucose level and insulin level 120 minutes later were measured. In the group to which “Insulina dried leaves / stem / seed powder” was administered, an increase in blood glucose level was clearly suppressed (Table).
5).

In the present invention, the results of animal experiments and human clinical tests using a diabetes model confirmed that the indigo preparation and its composition exerted a sufficient effect on the prevention and treatment of diabetes. In particular, it promotes the normalization of the action of insulin and the interaction with the receptor, improving glucose metabolism,
It is considered that metabolism was more clearly shown.

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Claims (2)

[Claims] 1. An extract of "Sukumo" which is produced from indigo leaves and stems belonging to the Polygonaceae family in the process of producing indigo dye 2. A fine powder obtained by crushing dried indigo leaves, stems and seeds belonging to the Polygonaceae plant by an appropriate means.