JP2002173442A - Antifatigue composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a functional food material containing extracts obtained from fish, shellfish, poultry, meat, etc. SOLUTION: An antifatigue composition having performance-enhancing and antifatigue effects is produced by using imidazole dipeptides and/or their salts as active components. The imidazole dipeptide of the antifatigue composition is preferably one or more compounds selected from anserine, carnosine and valenine. The content of the imidazole dipeptide and/or its salt is preferably 10-100 mass%.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an anti-fatigue composition using imidazole dipeptides obtained from fish, shellfish, poultry meat, animal meat and the like.

[0002]

2. Description of the Related Art When intense exercise is performed, glucose derived from glycogen in muscle is used, and an anaerobic glycolysis reaction proceeds to synthesize ATP necessary for muscle contraction. Then, lactic acid, which is a metabolite thereof, accumulates to cause a decrease in intramuscular pH, and the efficiency of muscle contraction decreases. This condition is called metabolic acidosis, and as a result,
It is thought to lead to muscle fatigue. Therefore, in order to suppress muscle fatigue due to continuous exercise, it is important not to cause metabolic acidosis.

[0003] In recent years, creatine has been actively taken as a supplement among athletes. Creatine is converted to creatine phosphate in the body and has the function of transferring energy as a high-energy phosphate compound,
It is also an essential component of ATP synthesis, and it is known that ingestion of creatine increases the creatine content of muscle and improves the performance of high-intensity exercise (Ha
rris, RC et al: Clin. Sci., 83, 367-374, 199
2).

[0004] In addition, when glycogen as an energy source is insufficient, body fat is more easily used and blood amino acids are converted into energy. Attempts have been made to improve muscle fatigue and muscle weakness. Amino acids particularly easily used as energy are branched-chain amino acids (leucine, isoleucine, and valine). For example, JP-A-58-16577, JP-A-7-25838 and JP-A-2000-26290 disclose the branched chain amino acids. It has been proposed to use amino acids as foods.

Further, Japanese Patent Application Laid-Open Nos. Hei 8-198748 and Hei 9-249556 disclose amino acids contained in saliva secreted by larvae of hornets (Vespar) for the purpose of improving motor function and recovering from fatigue. It has been proposed to suppress fluctuations in blood amino acids by administering an amino acid composition configured in accordance with the composition.

[0006]

By the way, extracts obtained from fish, shellfish, poultry meat, animal meat and the like contain various amino acids and are generally widely used as seasonings. Has been little studied.

Accordingly, an object of the present invention is to provide fish, seafood, poultry,
An object of the present invention is to provide a functional food material using extracts obtained from meat or the like.

[0008]

Means for Solving the Problems In the course of studying the physiologically active function of a seasoning extract prepared from skipjack and tuna meat, the present inventors have focused on imidazole dipeptides contained in the extract in large amounts. As a result of intensive studies, they have found that imidazole dipeptides have an effect of improving exercise ability, that is, an anti-fatigue effect, and based on this fact, have completed the present invention.

The anti-fatigue composition of the present invention is characterized by containing an imidazole dipeptide and / or a salt thereof as an active ingredient.

In the present invention, the imidazole dipeptides are preferably at least one selected from anserine, carnosine and varenin. Further, it is preferable to contain the imidazole dipeptides and / or salts thereof in an amount of 10 to 100% by mass.

According to the present invention, a new anti-fatigue composition using extracts obtained from fish, shellfish, poultry, livestock meat and the like which have been conventionally used as seasonings can be provided.

[0012]

BEST MODE FOR CARRYING OUT THE INVENTION The imidazole dipeptides which are the active ingredients of the anti-fatigue composition of the present invention mainly include anserine (β-alanyl-1-methylhistidine), carnosine (β-alanylhistidine), and varenin ( β-alanyl-3-methylhistidine). The anti-fatigue composition of the present invention preferably contains at least one or more selected from anserine, carnosine, and varenin as an active ingredient.

Examples of the salts of the imidazole dipeptides include salts of hydrochloric acid, lactic acid, acetic acid, sulfuric acid, citric acid, ascorbic acid, malic acid, succinic acid, adipic acid, gluconic acid, tartaric acid and the like.

The above imidazole dipeptides include fish meat,
It is contained in chicken, animal meat, etc., and can be obtained by purifying an extract extracted therefrom by a method such as water extraction, hot water extraction, alcohol extraction, supercritical extraction and the like.

For example, anserine can be obtained as follows. First, skipjack, tuna,
An extract is prepared from meat such as cows and chickens, and water is appropriately added to adjust the Brix (Bx.) Of the extract to 1 to 10%. Then, an ultrafiltration membrane (fraction molecular weight 5,000 to 50,
000) to remove the high molecular weight protein and collect the low molecular weight peptide fraction. Then, the literature (Suyama et al:
Bull. Japan. Soc. Scient. Fish., 33, 141-146, 196
According to the method of 7), the appropriately concentrated low molecular peptide fraction is subjected to ion exchange chromatography using a strongly acidic resin, and an eluate is collected. Then, the eluate can be obtained by desalting, adjusting the pH, and drying by freeze-drying or the like.

Carnosine is made from pork meat.
Valenin is made from whale meat (eg baleen whales)
It can be obtained by a method similar to the above.

The anti-fatigue composition of the present invention comprises the imidazole dipeptide and / or a salt thereof in an amount of 10 to 100% by mass.
It is preferably contained, more preferably 50 to 100% by mass.

Further, in addition to the above basic components, excipients, minerals, vitamins, sugars, fragrances and the like can be appropriately contained.

The product form of the anti-fatigue composition of the present invention includes tablets, powders, granules, solutions, capsules and the like.
There is no particular limitation.

The effective daily intake of the anti-fatigue composition of the present invention is 1 to 200 m in terms of imidazole dipeptides.
g / kg of body weight, more preferably 5-30 mg / kg of body weight
It is.

Further, the anti-fatigue composition of the present invention may be added to foods and drinks such as soft drinks, tablet confections, snacks, powdered soups, sausages and the like. In that case, the addition amount per serving is 100 to 2,000 mg in terms of imidazole dipeptides.
Is preferable, and 400 to 2,000 mg is more preferable.
If the amount of imidazole dipeptides is less than 100 mg, the anti-fatigue effect cannot be expected so much, and 2,000 mg
If the amount is too large, the taste of food and drink is affected, which is not preferable.

[0022]

The present invention will be specifically described below with reference to examples. Example 1 (Preparation of anserine) Skipjack extract (Bx. = 55%) prepared according to a conventional method
After diluting 10 kg with 4 volumes of water, the high molecular weight protein was removed using an ultrafiltration membrane (fraction molecular weight 10,000), and the low molecular weight peptide fraction was recovered. This fraction was concentrated and a 0.38N aqueous sodium citrate solution (p
H4.0) equilibrated with strong acidic resin (trade name “Amberlit
e IR-120 ", manufactured by Rohm and Haas Co., Ltd.).
0-fold 0.38N aqueous sodium citrate solution (pH 4.
The eluted fraction was eluted in 0) and collected. Then, the solution was desalted, adjusted to pH, and freeze-dried to obtain 45 g of powder.
I got When the obtained powder was analyzed by an amino acid analyzer manufactured by Hitachi, the purity was 98% as anserine hydrochloride.

Example 2 (Preparation of Carnosine) An extract (Bx. = 30) prepared from pig meat according to a conventional method
%) 24 g of powder was obtained in the same manner as in Example 1 except that 10 kg was used. This powder was 92% pure as carnosine hydrochloride.

Example 3 (Preparation of Valenin) An extract (B) prepared from beef whale meat according to a conventional method
x. = 50%) Except that 10 kg was used, 40 g of powder was obtained in the same manner as in Example 1. This powder was 92% pure as varenin hydrochloride.

Test Example (Forced Exercise Test Using Mice) Two groups of 20 6-week-old SPF mice (male) (10
After a 4 hour fast, the control group was given 200 mg / kg body weight of water for injection, and the test group was given the aqueous solution of anserine hydrochloride (40 mg / ml) prepared in Example 1 in terms of anserine hydrochloride. Each of them was orally administered orally to 200 mg / kg body weight.

Exactly one hour after the oral administration, the following forced exercise was applied to the mice. A water tank filled with water at a water temperature of 20 ° C. and air was blown in to make the water surface rippling (W265 × D
427 × H204 mm) and the swimming time was measured with the mouse. The mouse was loaded with a weight equivalent to 10% of the average weight of the mouse, and the time from the start of swimming until the mouse sank its head under the water surface for 7 seconds or more was defined as the swimming time.

Then, at the time when 3 minutes have passed after the measurement of the swimming time (the first time) and at the time when 30 minutes have passed (2
In the second round), the mouse was hung on a wire, and the time until the mouse fell was measured.

Further, one hour after the exercise load, blood was collected to separate the plasma, and the amount of lactic acid in the plasma was measured. The amount of lactic acid was measured using a commercially available kit (trade name: F-kit L-lactic acid, manufactured by Boehringer Mannheim). In addition, each of the above measurement results was represented by an average value ± standard error, and a test for a significant difference was performed by Student't t-test.

FIG. 1 shows the results of the swimming time. From FIG. 1, the test group tended to have a longer swimming time than the control group.

FIGS. 2 and 3 show the results of the first and second suspension times. 2 and 3, in each case, the test group tended to have a longer suspension duration than the control group. In particular, in the second suspension, the test group was found to have an effect of significantly increasing the suspension time at a risk factor of 0.01 or less relative to the control group.

FIG. 4 shows the amount of plasma lactate one hour after the exercise load. From FIG. 4, it can be seen that the test group had significantly lower plasma lactic acid levels at a risk rate of 0.05% or less than the control group.

The above results suggest that oral intake of anserine improves exercise performance.
In addition, since the amount of lactic acid in the plasma after exercise was kept low, it was suggested that muscle fatigue due to exercise was suppressed.

[0033]

As described above, according to the present invention,
By containing imidazole dipeptides and / or salts thereof as an active ingredient, an anti-fatigue composition having improved exercise performance and an anti-fatigue effect can be provided.

[Brief description of the drawings]

FIG. 1 is a diagram showing the results of measuring the swimming time of mice.

FIG. 2 is a diagram showing a result of a first suspension time measurement of a mouse.

FIG. 3 is a diagram showing the results of a second suspension time measurement of mice.

FIG. 4 is a diagram showing the amount of lactic acid in plasma of a mouse at the time point when one hour has passed after exercise load.

Claims (3)

[Claims] 1. An anti-fatigue composition comprising an imidazole dipeptide and / or a salt thereof as an active ingredient. 2. The anti-fatigue composition according to claim 1, wherein the imidazole dipeptide is at least one selected from anserine, carnosine and varenin. 3. The method according to claim 1, wherein the imidazole dipeptide and / or a salt thereof is contained in an amount of 10 to 100% by mass.
3. The anti-fatigue composition according to item 1.