JP2002338473A - Anti-fatigue composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a functional food material utilizing extracts obtained from fishes and shellfishes, chicken, meat, etc. SOLUTION: This anti-fatigue composition having antifatigue effect is obtained by including an imidazole compound and D-ribose as active ingredients. In the antifatigue composition, the imidazole compound includes, preferably, at least one or more kinds of histidine, anserine, carnosine, balenine and salts thereof. The composition comprises, preferably, 5-50 wt.% of the imidazole compound and 5-50 wt.% of the D-ribose.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to an anti-fatigue composition using an imidazole compound and D-ribose obtained from fish, shellfish, poultry, livestock meat and the like.

[0002]

2. Description of the Related Art When intense exercise is performed, glucose derived from glycogen in muscle is used, and an anaerobic glycolysis reaction proceeds to synthesize ATP necessary for muscle contraction. Then, lactic acid, which is a metabolite thereof, accumulates to cause a decrease in intramuscular pH, and the efficiency of muscle contraction decreases. This condition is called metabolic acidosis, and as a result,
It is thought to lead to muscle fatigue. Therefore, in order to suppress muscle fatigue due to continuous exercise, it is important not to cause metabolic acidosis.

[0003] In recent years, creatine has been actively taken as a supplement among athletes. Creatine is converted to creatine phosphate in the body and has the function of transferring energy as a high-energy phosphate compound,
It is also an essential component of ATP synthesis, and it is known that ingestion of creatine increases the creatine content of muscle and improves the performance of high-intensity exercise (Ha
rris, RC et al: Clin. Sci., 83, 367-374, 199
2).

[0004] In addition, when glycogen as an energy source is insufficient, body fat is more easily used and blood amino acids are converted into energy. Therefore, the concentration of blood amino acids is increased as much as possible and used as an energy source. Attempts have been made to improve muscle fatigue and muscle weakness. Amino acids particularly easily used as an energy source are branched-chain amino acids (leucine, isoleucine, and valine). For example, Japanese Patent Application Laid-Open Nos. It has been proposed to utilize chain amino acids as foods.

Further, Japanese Patent Application Laid-Open Nos. Hei 8-198748 and Hei 9-249556 disclose amino acids contained in saliva secreted by larvae of hornets (Vespar) for the purpose of improving motor function and recovering from fatigue. It has been proposed to suppress fluctuations in blood amino acids by administering an amino acid composition configured in accordance with the composition.

[0006]

By the way, extracts obtained from fish, shellfish, poultry meat, animal meat and the like contain various amino acids and are generally widely used as seasonings. Has been little studied.

Accordingly, an object of the present invention is to provide fish, seafood, poultry,
It is to provide a functional food material using extracts obtained from livestock meat and the like.

[0008]

Means for Solving the Problems The present inventors have focused on imidazole compounds contained in a large amount in a seasoning extract prepared from skipjack or tuna meat in the course of studying the physiologically active function of the extract. As a result of intensive studies, they have found that the imidazole compound has an anti-fatigue effect. Furthermore, they have found that a better anti-fatigue effect can be obtained by using the imidazole compound and D-ribose in combination, and based on these facts, have completed the present invention.

The anti-fatigue composition of the present invention is characterized by containing an imidazole compound and D-ribose as active ingredients.

In the present invention, the imidazole compound is preferably at least one selected from histidine, anserine, carnosine, varenin and salts thereof.

The imidazole compound may be used in an amount of 5 to 50.
It is preferable to contain 5 to 50% by mass of the D-ribose.

According to the present invention, muscle fatigue during or after exercise is obtained by ingesting orally using extracts obtained from fish, shellfish, poultry, animal meat, etc. which have been conventionally used as seasonings. It is possible to provide an anti-fatigue composition capable of reducing the like.

Further, in the present invention, it is preferable to further contain at least one selected from taurine, creatine, vitamin E, vitamin C, carotenoid, reduced glutathione, and minerals. According to this aspect, it is possible to provide an anti-fatigue composition provided with the physiologically active effect of each of the above components.

[0014]

BEST MODE FOR CARRYING OUT THE INVENTION As the imidazole compound which is one of the active ingredients of the anti-fatigue composition of the present invention, specifically, histidine, anserine (β-alanyl-1-methylhistidine), carnosine (β-ara Nylhistidine),
Valenin (β-alanyl-3-methylhistidine) and the like. In the present invention, it is preferable that at least one selected from histidine, anserine, carnosine, varenin and salts thereof is contained as an active ingredient. Further, as the above salts, hydrochloric acid, lactic acid, acetic acid,
Salts such as sulfuric acid, citric acid, ascorbic acid, malic acid, succinic acid, adipic acid, gluconic acid, tartaric acid and the like can be mentioned.

The above imidazole compound is used for fish meat, chicken meat,
It is contained in meat, etc., from which water extraction, hot water extraction,
It can be obtained by purifying an extract extracted by a method such as alcohol extraction or supercritical extraction.

For example, anserine can be obtained as follows. First, skipjack, tuna,
An extract is prepared from meat such as cows and chickens, and water is appropriately added to adjust the Brix (Bx.) Of the extract to 1 to 10%.
000) to remove the high molecular weight protein and collect the low molecular weight peptide fraction. Then, the literature (Suyama et al:
Bull. Japan. Soc. Scient. Fish., 33, 141-146, 196
According to the method of 7), the appropriately concentrated low molecular peptide fraction is subjected to ion exchange chromatography using a strongly acidic resin, and an eluate is collected. Then, the eluate can be obtained by desalting, adjusting the pH, and drying by freeze-drying or the like.

Carnosine is made from pork as a raw material.
Valenin is made from whale meat (eg baleen whales)
It can be obtained by a method similar to the above.

The anti-fatigue composition of the present invention preferably contains 5 to 50% by mass of the above imidazole compound.
More preferably, it contains 50% by mass. When the content of the imidazole compound is less than 5% by mass, a sufficient anti-fatigue effect cannot be expected.

D-ribose, another active ingredient of the anti-fatigue composition of the present invention, is a monosaccharide belonging to aldopentoose and naturally exists as a nucleoside or nucleotide by binding to phosphate. In the present invention,
A commercially available product can be used, and can be purchased, for example, from Wako Pure Chemical Industries, Ltd.

The anti-fatigue composition of the present invention preferably contains D-ribose in an amount of 5 to 50% by mass, more preferably 10 to 50% by mass.
More preferably, it is included. If the D-ribose content is less than 5% by mass, a sufficient anti-fatigue effect cannot be expected, and
If it is more than mass%, problems such as moisture absorption and coloring occur, which is not preferable.

The mixing ratio of the above-mentioned imidazole compound and D-ribose is based on the mass ratio (imidazole compound: D-ribose).
Ribose) is preferably 1:10 to 10: 1. If the mixing ratio of the imidazole compound and D-ribose is outside the above range, a synergistic anti-fatigue effect cannot be expected.

The anti-fatigue composition of the present invention preferably further contains at least one selected from taurine, creatine, vitamin E, vitamin C, carotenoid, reduced glutathione, and minerals. By blending the above components, the physiologically active effects of each component, for example, anti-fatigue effects such as taurine and creatine, and antioxidant effects such as vitamin E, vitamin C, carotenoid, reduced glutathione, etc. can be expected. In addition, as said minerals,
Trace metal elements that can be used in food and drink, such as calcium,
Examples include sodium, magnesium, selenium, iron, zinc and the like.

The anti-fatigue composition of the present invention can appropriately contain excipients, sugars, fragrances, pigments, oils and fats, proteins and the like in addition to the above basic components.

The product form of the anti-fatigue composition of the present invention is not particularly limited and can be appropriately selected according to the use form, and examples thereof include tablets, powders, granules, capsules, pastes, and solutions.

The effective daily intake of the anti-fatigue composition of the present invention is 1 to 200 mg / kg of body weight, more preferably 5 to 5 in terms of the total amount of the imidazole compound and D-ribose.
30 mg / kg of body weight.

The anti-fatigue composition of the present invention may be added to foods and drinks such as soft drinks, tablets, snacks, powdered soups and sausages. In that case, the amount added per serving is 10 in terms of the total amount of the imidazole compound and D-ribose.
The amount is preferably from 0 to 2000 mg, more preferably from 400 to 2000 mg. If the total amount of the imidazole compound and D-ribose is less than 100 mg, the anti-fatigue effect cannot be expected so much, and if it exceeds 2000 mg, the taste of food and drink is adversely affected, which is not preferable.

[0027]

The present invention will be specifically described below with reference to examples. Example 1 Skipjack extract (Bx. = 55%) prepared according to a conventional method
After diluting 10 kg with 4 times the volume of water, the high molecular weight protein was removed using an ultrafiltration (UF) membrane (molecular weight cut off 10,000), and a low molecular weight peptide fraction was collected.
This fraction was concentrated and subjected to column chromatography packed with a strongly acidic resin (trade name “Amberlite IR-120”, manufactured by Rohm and Haas) equilibrated with a 0.38N aqueous sodium citrate solution (pH 4.0). The solution was eluted with a 0.38N aqueous sodium citrate solution (pH 4.0) 4.5 to 6.0 times the resin volume to collect an eluted fraction (Suya
ma etal: Bull. Japan. Soc. Scient. Fish., 33, 141-
146, 1967). Then, the solution was desalted, adjusted for pH, and freeze-dried to obtain 45 g of powder. When this powder was analyzed with a Hitachi amino acid analyzer,
The purity was 98% as anserine hydrochloride.

Example 2 The following animal experiments were performed using anserine hydrochloride prepared in Example 1 and D-ribose (manufactured by Wako Pure Chemical Industries, Ltd.).

Forty male SPF mice, 6 weeks old, were divided into 4 groups (10 mice in each group) and fasted for 4 hours. After that, water for injection was used for the control group, and an aqueous solution of anserine hydrochloride (for the anserine administration group). 40 mg / ml) by 20 as anserine hydrochloride.
0 mg / kg body weight, D
-Anserine-ribose mixture administration group is a mixture of anserine hydrochloride and D-ribose in a mass ratio of 1: 1 so that an aqueous solution of ribose (40 mg / ml) becomes 200 mg / kg body weight in terms of D-ribose. Aqueous solution (4
(0 mg / ml) was forcibly administered orally so that the total amount of anserine hydrochloride and D-ribose was 200 mg / kg body weight.

Mice were subjected to the following forced exercise exactly one hour after oral administration. A water tank (polysulfone cage, outer diameter 265W × 427D × 204Hmm) filled with water at a water temperature of 20 ° C and fed with air to make the water surface ruffled.
Then, the mouse was put into the tank and the swimming time was measured. In addition,
The mouse was loaded with a weight equivalent to 10% of the average weight of the mouse, and the time from the start of swimming until the mouse sank its head under the water surface for at least 7 seconds was measured as the swimming time.

After the above exercise load, blood is collected to separate plasma, and the amount of lactic acid in the plasma is measured using a commercially available “F-kit L”.
-Lactic acid "(trade name, manufactured by Boehringer Mannheim Co., Ltd.). Each of the above measurement results is represented by an average value ± standard error, and a test for a significant difference was performed using Student's t-te
Performed by st.

FIG. 1 shows the results of the swimming time of each group. From FIG. 1, the anserine administration group,
In the ribose-administered group and the anserine-ribose mixture-administered group, the swimming time tended to be longer. In particular, it can be seen that the anserine-ribose mixture administration group has a significantly longer swimming time than the other groups.

FIG. 2 shows the plasma lactate level after exercise. 2, the anserine administration group (23.9% decrease), the ribose administration group (29.4% decrease), and the anserine-ribose mixture administration group (42.0%) as compared to the control group.
% Decrease) is found to be significantly lower.

From the above results, the dose of each group (200 m
g / kg body weight), it is suggested that fatigue can be reduced synergistically by using anserine and D-ribose together, compared to the case of using anserine or D-ribose alone. Was done.

Example 3 An example of the formulation of a food or drink containing the anti-fatigue composition of the present invention is shown. Table 1 shows a sports nutritional drink, and Table 2 shows a formulation example of a tablet.

[0036]

[Table 1]

[0037]

[Table 2]

[0038]

As described above, according to the present invention, an anti-fatigue composition having an excellent anti-fatigue effect can be provided by containing an imidazole compound and D-ribose as active ingredients.

[Brief description of the drawings]

FIG. 1 is a diagram showing the results of measuring the swimming time of mice.

FIG. 2 is a graph showing the amount of lactic acid in the plasma of mice after exercise.

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Claims (4)

[Claims] 1. An anti-fatigue composition comprising an imidazole compound and D-ribose as active ingredients. 2. The anti-fatigue composition according to claim 1, wherein the imidazole compound is at least one selected from histidine, anserine, carnosine, varenin and salts thereof. 3. The anti-fatigue composition according to claim 1, comprising 5 to 50% by mass of the imidazole compound and 5 to 50% by mass of the D-ribose. 4. Taurine, creatine, vitamin E, vitamin C, carotenoid, reduced glutathione,
2. The composition according to claim 1, which comprises at least one selected from minerals.
4. The anti-fatigue composition according to any one of items 1 to 3.