JP2002087980A - Sodium ion-discharging stimulator and food containing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a sodium ion-discharging stimulator capable of discharging excessively taken sodium chloride extracorporeally in a positive and safe manner, and a food containing the stimulator. SOLUTION: The sodium ion-discharge stimulator containing at least one kind out of a water extract and a hot water extract of a Phaeophyceae plant (Laminaria, Kjellmaniella and Myrionema, Nemacystis are excluded), and their metal salts (sodium salt is excluded) as an active ingredient, and the food containing the same.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a sodium ion excretion enhancer and a food containing the same, and more particularly to a sodium ion excretion enhancer, which inhibits the absorption of sodium ions ingested in the gastrointestinal tract to prevent sodium ions from being excreted in feces The present invention relates to a sodium ion excretion promoter excellent in promoting excretion into food and its use in foods.

[0002]

2. Description of the Related Art According to the results of the National Nutrition Survey issued by the Ministry of Health and Welfare, the salt consumed by Japanese in one day every year since 1975
It was 1.5 g or more, especially 12.8 g in 1993. On the other hand, since there is a correlation between the daily salt intake and the incidence of hypertension, the Ministry of Health and Welfare has decided to reduce the daily salt intake in order to prevent the occurrence of hypertension and eventually the occurrence of stroke. It is recommended that the weight be 10 g or less. In the United States, as in Japan, daily salt intake is restricted.
The United States Joint Committee recommends that patients with hypertension have a daily salt intake of 6 g or less.

It is said that there is also a correlation between salt intake and mortality from gastric cancer. In regions where salt intake is high, such as Toyama City and Hirosaki City, the mortality from gastric cancer is high. In areas where salt intake is low, such as Beppu City,
In Okinawa City, etc., data have been obtained that the mortality rate from gastric cancer is low.

[0004] On the other hand, the fact that dietary fiber such as alginate has a certain sodium ion-adsorbing ability is described in the Journal of the Japanese Society of Home Economics Vol.39, No.3, pp.187-195 (1988).
In addition, it is disclosed in Japanese Patent Laid-Open No. 1-15 that an aqueous extract of kelp is effective for prevention and treatment of hypertension, and alginic acid which is a dietary fiber of kelp has an effect of suppressing uptake of salt.
No. 7363 discloses that a hot water extract of Mozuku has a sodium ion adsorption ability.
No. 0 publication.

[0005]

As described above, since excessive salt in the body adversely affects the human body, the absorption of salt into the body is effectively inhibited, and the excessive salt is excreted outside the body. Although technology is being developed, its adsorptive capacity is not yet satisfactory, or it has an adverse effect on fecal properties when excreting sodium ions into feces, so a better sodium ion excretion enhancer has been developed. Development is awaited.

[0006] Accordingly, an object of the present invention is to provide a sodium ion excretion promoter capable of positively and safely excreting excessively ingested salt outside the body, and a food containing the same.

[0007]

The inventors of the present invention have conducted various studies to find components having the above-mentioned effects from a wide range of natural materials, industrial materials, and the like. (Excluding sodium salts) have excellent sodium ion excretion promoting ability and can be used as pharmaceuticals and foods, and have completed the present invention.

That is, the present invention relates to a brown algae (however, kelp,
Water extract and hot water extract of trollocum and mozuku, and their metal salts (excluding sodium salts)
A sodium ion excretion enhancer comprising at least one of the above as an active ingredient, and a food containing the same.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION The brown algae (Phae) used in the present invention
ophyceae) (excluding kelp, trollocum and mozuku)
Various types can be used, but preferably are those of the order Fukuronori (for example, Fukuronori, Kayamonori, etc.), and the order of the Nagamumo (for example, Namakikura, Chromo etc.),
The order of the order Kombu (eg, seaweed, alame, etc., but excluding the case of seaweed and trollom) and the order of Hibamata (eg, hijiki, hibamata) can be mentioned, and particularly preferred are wakame and hijiki. Hereinafter, these “water extract or hot water extract of brown algae (excluding kelp, trollocum and mozuku)” may be simply referred to as “brown algae extract”.

When the brown alga extract of the present invention is extracted from the above brown algae with water, for example, room temperature water to 40 ° C., preferably room temperature water to 25 ° C., for 30 minutes to 3 hours, preferably 1 hour. It can be obtained by extracting about time. In the case of hot water extraction, for example, 40 ° C to 100 ° C, preferably 8 ° C
It can be obtained by extracting with hot water at 0 ° C. to 90 ° C. for 30 minutes to 3 hours, preferably for about 1 hour.

The brown algae extract of the present invention may be a metal salt (excluding sodium salts; hereinafter, unless otherwise specified, a metal salt except for sodium salts). The brown algae extract is mainly a sodium salt, but when this sodium salt is used as another metal salt, the above water extract or hot water extract is cooled, and a metal halide such as calcium chloride is added. Then, after stirring and completely dissolving, if necessary, low molecular weight substances are removed by dialysis, and the mixture can be left for a certain period of time, usually 2 to 24 hours.

Examples of usable metals include alkali metals such as potassium (excluding sodium), alkaline earth metals such as calcium and magnesium, and metals such as iron. Of these, potassium and calcium are preferred.

The resulting brown algae extract may be used as it is, but may be further purified, if necessary, by alcohol precipitation, ion exchange resin chromatography, gel filtration chromatography or the like.

Further, instead of the water extract or the hot water extract of the brown algae obtained as described above, a commercially available equivalent of the brown algae extract may be used.

The preferred physical properties of the brown algae extract or the metal salt thereof of the present invention are as follows. (1) Weight average molecular weight: 10,000 to 2,000,000
00, more preferably 30,000 to 700,000 (2) Sugar content: greater than 25%, more preferably 30 to 7
0% (3) Uronic acid content relative to constituent sugars: greater than 65%, more preferably 70 to 90%

The brown algae of the present invention or a metal salt thereof has an ability to inhibit sodium ion absorption, and particularly, when taken orally, inhibits the absorption of sodium ions in the digestive tract and promotes its excretion. Have. Therefore, the brown algae or the metal salt thereof of the present invention is effective as a sodium ion excretion promoter.

The sodium ion excretion enhancer containing the brown algae or the metal salt thereof of the present invention is produced by processing the brown algae or the metal salt obtained by the above method into various forms by a usual method. Can be. For example, solids, liquids, emulsions, pastes, etc.

The sodium ion excretion enhancer of the present invention can be contained in various foods and the like. In particular,
Various diseases caused by excessive intake of salt, such as hypertension, stomach cancer, stroke, renal failure, and osteoporosis, can be used to ease the restriction of salt on patients, and is useful as a food for patients during its prevention and treatment. is there. In addition, since a large amount of sodium ions are excreted in feces, it is particularly effective for patients with reduced excretion of sodium ions from the kidney such as renal failure.

The food containing the sodium ion excretion enhancer of the present invention includes those which can be eaten immediately as it is, those which are eaten by cooking, etc., and those premixed for food production. It is. The solid product may be any of powder, granule and solid. Examples thereof include biscuits, cookies, cakes, snacks, confectionery such as rice crackers, bread, and powdered beverages (powdered coffee, cocoa, etc.). included. Examples of liquid, emulsified, and paste-like materials include juices, carbonated beverages,
Various drinks such as lactic acid bacteria drinks are included.

The brown algae or the metal salt thereof according to the present invention comprises about 10
It has the ability to excrete and absorb about 1 g of sodium salt (about 400 mg of sodium ions) per gram. Therefore, using this as a guide, it can be converted to the above brown algae or its metal salt in an amount of about 0.5 g to 50 g per day.
It is good to take about g.

[0021]

EXAMPLES The present invention will be described in more detail with reference to the following Examples, but it should not be construed that the present invention is limited thereto.

Example 1 The effects of the hijiki extract, the calcified hijiki extract and the seaweed extract on fecal and urinary sodium ion excretion of normal rats were compared and studied.

(1) Production method of each extract (1-1) Hijiki extract Hijiki powder was used as an example of the original alga Hijiki. 100 g of Hijiki powder (dried product) W. After being immersed in 1500 ml for 1 hour and sufficiently immersed in water, the suspension was kept at 90 ° C. for 1 hour to perform hot water extraction. The suspension was allowed to cool to room temperature, and water-insoluble components (seaweed residues) were removed by centrifugation (15000 G, 10 minutes). Supernatant (about 120
0 ml) using a rotary evaporator to 200 ml
200 ml of ethanol was added to the concentrated solution with stirring (alcohol concentration 50% (V /
V)). The precipitate generated by the alcohol precipitation was collected by centrifugation (15000 G, 10 minutes), and the collected precipitate was further washed with ethanol. The obtained sample was dried by drying under reduced pressure (50 ° C.),
6.60 g were obtained.

(1-2) Calcified Hijiki Extract Hijiki powder was used as an example of the original alga Hijiki. 100 g of Hijiki powder (dried product) W. After being immersed in 1500 ml for 1 hour and sufficiently immersed in water, the suspension was kept at 90 ° C. for 1 hour to perform hot water extraction. The suspension was allowed to cool to room temperature, and water-insoluble components (seaweed residues) were removed by centrifugation (15000 G, 10 minutes). Supernatant (about 120
0 ml), 10 g of CaCl ₂ .2H ₂ O was added, and the mixture was stirred and dissolved. Precipitated substances were produced. The suspension was concentrated to about 200 ml using a rotary evaporator, and 200 ml of ethanol was added to the concentrated liquid while stirring (alcohol concentration 50% (V / V)). The precipitate was collected by centrifugation (15000 G, 10 minutes), and the collected precipitate was further washed with ethanol. The obtained sample was dried by drying under reduced pressure (50 ° C.) to obtain 17.18 g of a hot water extract of calcified hijiki.

(1-3) Wakame Extract In the same manner as in the case of the hijiki extract, 15.0 g of wakame hot water extract was obtained using 100 g of wakame powder (dried product).

(2) Measurement of Molecular Weight The weight average molecular weight was calculated by a gel filtration method under the following conditions. <HPL conditions> Column: TSKgel GMPW _XL (manufactured by Tosoh Corporation) Solvent: 0.2 M phosphate buffer (pH 6.79) Flow rate: 0.5 ml / min Temperature: room temperature Detection: 210 nm Apply volume: 100 μl Measured by this method The weight-average molecular weights of the above-mentioned extract and calcified hijiki extract were 44,000 to 64.
The weight average molecular weight of the seaweed extract was about 200,000.

(3) Measurement of sugar content As reagents, (a) concentrated sulfuric acid (special grade reagent), (b)
A 5% aqueous phenol solution (new, not yellowish) was used. Galactose (special grade reagent grade)
Were dissolved in distilled water to a concentration of 20 μg to 600 μg / ml, respectively. Five solutions with different concentrations were selected from within this range. 0.5 ml of each solution was placed in a test tube, and 0.5 ml of the above (b) was added thereto, followed by sufficient stirring. Further, 2.5 ml of the above (a) was added, stirred, and allowed to stand at room temperature for 15 minutes. Then, the absorbance at 480 nm was measured by an absorbance analyzer. A calibration curve graph of the sugar concentration and the absorbance at 480 nm was prepared. Subsequently, 150 μg of each of the above-mentioned hijiki extract and wakame extract was accurately weighed and dissolved in 10 ml of pure water. 0.5 ml of the solution was placed in a test tube, and (b) was added to 0.
5 ml was added and stirred well. Furthermore, (a) is 2.5 m
After stirring for 15 minutes at room temperature, the absorbance at 480 nm was measured with an absorbance analyzer. The sugar concentration was calculated from the previously prepared calibration curve. On the other hand, for the sample used for measuring the sugar content, the moisture content was calculated using an infrared moisture analyzer, and the sugar content was analyzed after weight correction. The sugar content of the Hijiki extract measured by the above method was 40.3%, the sugar content of the Calcified Hijiki extract was 38.3%, and the sugar content of the Wakame extract was 26.9%.

(4) Measurement of uronic acid Quantification is performed based on an infrared absorption peak (1630 cm ⁻¹ ) due to a —COOH group that is peculiar to uronic acid. This is based on the fact that the ratio of the amount of -OH groups to the amount of -COOH groups present in the sugar chain substantially corresponds to the peak area ratio. First, glucose was selected as a control (uronic acid content 0%), and glucose / glucuronic acid was adjusted to 20/80, 40/60, 60/4.
The IR spectra of the samples mixed at a weight ratio of 0, 80/20 and 100/0 (w / w) were measured using an infrared absorption spectrum spectrometer FT / IR-230 (manufactured by JASCO Corporation). The area of the absorption peak attributed to the -OH group and the area of the absorption peak attributed to the -COOH group of the obtained spectrum were determined, and the calibration curve was prepared by taking the amount of uronic acid on the horizontal axis and the peak area ratio on the vertical axis. . On the other hand, a sample of glucose / measurement sample = 50/50 (w / w) was prepared, and the IR spectrum was measured in the same manner. -Of the obtained spectrum
The area ratio of the absorption peak attributable to the OH group and the area of the absorption peak attributable to the -COOH group was determined, and the area ratio was determined. The uronic acid amount of the measurement sample was determined based on the previously determined calibration curve. The uronic acid content of the hijiki extract measured by the above method was 70.1% based on the constituent sugars, and the uronic acid content of the wakame extract was 97.1% based on the constituent sugars.

(6) Materials and Methods 9-week-old male Wistar rats (Charles River Japan) were used as experimental animals. The animals were fasted overnight and then transferred to individual metabolism gauges for 3 days, cellulose diet @ casein 20% (same below w / w), α corn starch 69.5%, soybean oil 5%, sucrose 5%,
AIN-76 Vitamin Mixture 1%, AIN-76 Mineral Mixture 3.5%, Salt 1% and Cellulose 5% were used to acclimate to a powdered diet under restricted feeding (20 g / rat / day). Thereafter, the weights of the animals were measured, and the animals were divided into groups (n = 5 / group) based on the food intake and body weight during the training period. That is, the control group was a cellulose group ingested with a cellulose diet, and the test diet group was a 2% addition group in which a diet in which cellulose in the cellulose diet was replaced by 40% with each test substance or a test diet in which the total amount was replaced, respectively. % Addition groups were provided respectively.

After the rats in each group were fasted overnight, each test diet was bred for 2 days under a restricted feed (20 g / rat / day). On the second day of feeding the test food, urine and feces were collected for 24 hours from 7:00 pm. For the 5% added group, the stool properties were recorded. During the test period, rats in each group were allowed to freely take in distilled water. The feces ingested were 7
After performing a drying treatment at 0 ° C. for 4 days to determine a dry weight, the resultant was ashed (500 ° C. or more, 36 hours), and sodium was measured by an atomic absorption method. The urine collected was measured for urinary sodium by an ion electrode method assuming a specific gravity of 1.0 from the difference in weight of the urine collection cup.

(7) Results (7-1) Hijiki Extract The sodium ion excretion in urine of the cellulose group was 97.9 m
g / day, 91.1 mg / day in the 2% group and 8% in the 5% group in the group receiving the hijiki extract.
At 2.3 mg / day, a dose-dependent decrease in urinary sodium ion excretion due to ingestion of the hijiki extract was observed. on the other hand,
The fecal sodium ion excretion was 0.2 mg / day in the cellulose group, whereas 4.5 mg / day in the 2% -added group and 13 in the 5% -added group in the group to which the hijiki extract was given. At 8 mg / day, an increase in fecal sodium ion excretion was observed in a dose-dependent manner due to the ingestion of the hijiki extract.

(7-2) Calcified Hijiki Extract and Wakame Extract The calcium-containing hijiki extract and wakame extract were also subjected to the feces for the 2% and 5% groups in the same manner as the above-mentioned Hijiki extract. The results of sodium ion excretion are shown in Table 1 below.

[0033]

[Table 1]

(7-3) Fecal Properties As shown in Table 2 below, no diarrhea symptoms were observed in any of the 5% added groups of the hijiki extract, the calciumated hijiki extract and the wakame extract.

[0035]

[Table 2]

(7-4) Conclusion From these results, the brown algae extract or its metal salt excretes the ingested sodium from the feces without affecting the stool properties and inhibits the absorption of sodium into the body. Has been shown to have

Example 2 (Preparation of fine granules) A mixture of 70 parts by weight of the swordfish extract or wakame extract, 20 parts by weight of lactose and 10 parts by weight of corn starch used in Example 1 was mixed with 5% of hydroxypropylmethylcellulose. Fluid bed granulation was carried out with an aqueous solution to obtain fine granules.

Example 3 (Production of biscuits) In the following examples, "parts" refers to parts by weight. Mix 8 parts of shortening and 18 parts of sugar, then 42 parts of light flour, 7.5 of the hijiki extract used in Example 1
, Baking powder 0.8 part, egg 16 parts, glucose 1 part and water 25 parts were added and stirred to make dough. The dough was rolled to a thickness of 5 mm, punched out so that one piece weighed 16 to 17 g, and baked in a 90 ° oven for 32 to 36 minutes. As a result, about 12 g of biscuit was obtained. It is calculated to contain 1 g of the Hijiki extract in 12 g of biscuit.
In other words, eating one biscuit gives about 40mg
Of sodium ion (about 100 mg in salt).

Example 4 (Manufacture of beverage) 12.5 g of granulated sugar, 0.2 g of citric acid crystals and 1 g of the hijiki extract used in Example 1 were made up to 100 ml with ion-exchanged water. 10
A beverage containing a pheasant extract was obtained by sterilizing for a minute.

[0040]

The brown algae extract of the present invention or its metal salt inhibits the absorption of sodium ions in the digestive tract, lowers the rate of absorption into the body, and excretes sodium ions in feces in particular. Since it has an excellent action, it can be contained in various foods and the like as an excellent sodium ion absorption inhibitor or sodium ion excretion promoter by containing an effective amount thereof. Furthermore, the stool properties are good.

By containing an effective amount of these brown algae extracts or metal salts thereof, it is effective for the prevention and treatment of diseases caused by excessive intake of salt or diseases requiring restriction of salt intake. In particular, the brown algae extract or the metal salt thereof of the present invention has a high sodium ion excretion amount in feces, and therefore is extremely effective for patients with reduced renal sodium ion excretion such as renal failure.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification code FI Theme coat ゛ (Reference) A61P 3/12 A23L 2/00 FF Term (Reference) 4B017 LC03 LG18 LP01 4B018 MD03 MD67 ME14 MF01 4C088 AA13 BA09 ZC21

Claims (9)

[Claims] Claims 1. An active ingredient comprising at least one of a water extract and a hot water extract of brown algae (excluding kelp, trollocum and mozuku) and their metal salts (excluding sodium salts). Characteristic sodium ion excretion enhancer. 2. An at least one of a water extract and a hot water extract of the order Fukuronori, Phytomorphidae, Laminaria (excluding kelp and trollocan) and the order Himata, and their metal salts (excluding sodium salts) A sodium ion excretion enhancer comprising, as an active ingredient. 3. A water extract and a hot water extract of seaweed and hijiki and their metal salts (excluding sodium salts)
A sodium ion excretion enhancer comprising at least one of the following as an active ingredient: 4. The sodium ion excretion enhancer according to claim 1, wherein the metal salt is an alkali metal salt (excluding a sodium salt) or an alkaline earth metal salt. 5. The sodium ion excretion enhancer according to claim 4, wherein the metal salt is a potassium salt or a calcium salt. 6. An active ingredient having a molecular weight of 10,000 to 2,
The sodium ion excretion enhancer according to any one of claims 1 to 5, wherein the amount is 1,000,000. 7. The active ingredient having a molecular weight of 10,000 to 2,
The sodium ion excretion enhancer according to any one of claims 1 to 5, wherein the sodium ion excretion enhancer is 1,000,000 and the sugar content is more than 25%. 8. An active ingredient having a molecular weight of 10,000 to 2,
And the sugar content is greater than 25%,
The sodium ion excretion enhancer according to any one of claims 1 to 5, wherein the uronic acid content is greater than 65% based on the constituent sugar. 9. A food having a sodium ion excretion promoting action, comprising the active ingredient according to any one of claims 1 to 8.