JP2002084883A - Method for culturing tricholom matsutake for transplantation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for culturing Tricholom matsutake for transplantation, by which the culture work of the Tricholom matsutake for the transplantation and the transplantation work of the Tricholom matsutake after the culture can simply be carried out. SOLUTION: The cylinder main body 2 of a culture cylinder 1 is inserted into a dent 8 which is formed in the main body 6 of a culture container 5 and is used for mounting the culture cylinder therein, and an agar culture medium 11 is charged in the culture cylinder. The agar culture medium 11 is inoculated with the spores of the Tricholom matsutake, and then covered with a cap 3B. The main body 6 of the container is covered with a container lid 7. The culture container 5 is subjected in the state to the culture of the Tricholom matsutake for the transplantation under prescribed atmosphere conditions. After the culture, the cylinder main body 2 is taken out and then planted in a target culture medium for the transplantation. Thus, the culture and transplantation of the Tricholom matsutake for the transplantation can be performed with the simple works.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for culturing Matsutake fungi for transplantation by purely cultivating Matsutake fungi for transplantation using an agar medium. The present invention also relates to a culture tube and a culture apparatus suitable for use in a method for culturing Matsutake fungi for transplantation. Furthermore, the present invention relates to a method for transplanting purely cultured Matsutake fungi for transplantation.

[0002]

2. Description of the Related Art Various methods have been tried for artificial culture and artificial propagation of Matsutake mushrooms for a long time. As one of these methods, matsutake fungi for transplantation are purely cultured using an agar medium, and the cultured matsutake fungi for transplantation are transplanted to a natural medium such as matsutakeyama, and artificially propagated and artificially cultivated matsutake. Methods are known.

In the pure culture method, a solid medium or a liquid medium is formed at the bottom of a test tube, a flask, a bottle, or the like, and after sterilization,
Inoculate Matsutake inoculum such as spores in the medium, and then
Matsutake fungi are left in an atmosphere suitable for growth and culture.

[0004] It has been confirmed that fruit body primordia can be produced from the matsutake hyphae thus pure-cultured.
No further results have been confirmed or reported. Therefore, in general, attempts have been made to transplant matsutake hyphae, which have been purely cultured, into a natural medium (white) to form mycorrhizas on the roots of Pinus densiflora, thereby artificially cultivating or growing matsutake. .

[0005]

Here, the matsutake mycelium thus purely cultured must be removed from a test tube or flask, transferred to a transplant destination, and planted there. However, such a porting operation is troublesome,
Another problem is that there is a high possibility that matsutake hyphae after transplantation will not grow due to contamination by various bacteria at the time of transplantation.

[0006] The object of the present invention is to solve the above problems.
It is an object of the present invention to provide a method for culturing Matsutake fungi for transplantation, which can perform pure culture of Matsutake fungi for transplantation in a form that allows easy transplantation.

Another object of the present invention is to propose a culture tube and a culture apparatus suitable for use in such a culture method.

Another object of the present invention is to propose a method for transplanting Matsutake fungi for transplantation cultured using the culture tube and the culture apparatus according to the present invention.

[0009]

Means for Solving the Problems In order to solve the above-mentioned problems, the present invention relates to a culture tube used for a method of culturing Matsutake fungi for transplantation, which comprises purely cultivating Matsutake species using a solid medium. The culture tube is: a tube body with a bottom;
It is characterized by having a lid detachably mounted on the upper end opening of the cylinder body; and a slit formed on the outer peripheral wall of the cylinder body.

[0010] Here, in order to easily inoculate the matsutake fungus on the solid medium contained in the cylinder body, the seed fungus having a rod that can be inserted into the cylinder body as the lid. It is desirable to have a planting lid.

[0011] In addition, in the case of pure culture, it is desirable to provide a lid with a vent hole as the lid in order to ensure air permeability to the solid medium in which the matsutake species are inoculated and prevent invasion of various bacteria. .

Further, when the Matsutake hypha is propagated to a transplant destination or the like after the culture container has been propagated, in order to protect the Matsutake hypha, a bottomed protective body into which the slit-forming portion of the cylinder body can be inserted. It is desirable to have a cylinder.

Next, the present invention relates to a culture apparatus used in a method for culturing Matsutake fungi for transplantation, which comprises purely cultivating Matsutake species using a solid medium. The culture apparatus comprises a culture vessel and at least one culture tube. have.

The culture vessel includes: a culture tube; a recess for mounting the culture tube into which the culture tube can be inserted from above; a container body in which the recess for mounting the culture tube is formed; A container lid detachably mounted on the opening side of the recess for use; a vent formed in the container main body; and a vent formed in the container lid. The culture tube includes: a tube main body having a bottom; a lid detachably mounted on an upper end opening of the tube main body; a slit formed in an outer peripheral wall of the tube main body; And a protective cylinder with a bottom that can be inserted.

On the other hand, the present invention relates to a method for cultivating Matsutake fungi for transplantation using the culturing apparatus having the above-mentioned configuration. The culturing method comprises the steps of: A step of inserting a solid medium into the tube main body after being inserted into the culture tube mounting concave portion; and a seed inoculation cover having a rod that can be coaxially inserted into the tube main body as the cover that covers the tube main body. Preparing a cover, covering the tube body with the lid, sterilizing in that state; and removing the inoculation seed inoculation lid,
Attaching a matsutake inoculum to the rod and inoculating the matsutake inoculum at the tip of the rod in a solid medium; and sterilizing the lid as a cover for covering the cylinder body in a state in which a vent portion is closed with a cotton plug. Preparing a lid with a vent hole, and covering the lid with the cylinder main body; and covering the container main body with the container lid that has been sterilized with the vent hole portion sealed with a cotton plug. In this state, the culture vessel is allowed to stand for a predetermined period under a predetermined atmosphere temperature and humidity for a predetermined period of time, so that a matsutake hypha is grown on a solid medium in each culture tube. I have.

Next, the method for transplanting Matsutake fungi of the present invention comprises:
The culture tube in a state in which the matsutake mycelium has been grown by the method for culturing Matsutake fungi for transplantation is removed from the culture tube mounting recess of the culture container, and the culture tube is put into the protection tube. It is characterized in that the culture tube is transported to a transplant destination.

In this case, at the transplant destination, the culture tube may be extracted from the protective tube and embedded in the transplant medium.

[0018]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, an example of a method for culturing Matsutake fungi for transplantation to which the present invention is applied and a method for transplanting Matsutake fungi obtained by the method will be described with reference to the drawings.

(Culture apparatus of Matsutake fungi for transplantation) An example of a culture apparatus suitable for use in the method of culturing Matsutake fungi for transplantation of the present embodiment will be described with reference to FIGS.

FIG. 1 is a perspective view showing an example of a culture tube which is a component of the culture apparatus of the present embodiment, FIG. 2 is an exploded perspective view thereof, and FIG. 3 is a sectional view.

The culture tube 1 of this embodiment is made of plastic,
A cylinder main body 2, a lid 3, and a protection cylinder 4 are provided. Tube body 2
Has a cylindrical upper portion 21 and a frusto-conical lower portion 22 converging downward, between which a large-diameter flange 23 is formed. The lower portion 22 has a circular bottom plate portion 24 and an outer peripheral wall portion 25, and the outer peripheral wall portion 25 is formed with a plurality of slits 26 extending vertically at equal angular intervals.

The lid 3 includes a circular lid main body portion 31, a cylindrical portion 32, and a knob portion 33 formed on the upper surface of the lid main body portion 31. This lid 3 is attached to the upper end opening 2 of the cylinder body 2.
The size of each part is set so that the upper part 21 of the cylinder main body just fits inside the cylindrical part 32 in a state where the cylindrical part 32 is covered, and the lower end edge of the cylindrical part 32 contacts the flange 23. .

On the other hand, the protective cylinder 4 is a truncated cone-shaped cylinder with a bottom having an open upper end, into which the lower part 22 of the cylinder body can be inserted, and the lower part 22 can be inserted. In this state, the lower surface of the flange 23 rides on the upper opening edge of the protective cylinder 4.

Here, the culture tube 1 of the present embodiment is provided with a seed inoculation cover 3A that can be put on the tube main body 2 like the cover 3, and a cover 3B with a vent. The seed inoculation lid 3A has a rod 34 for inoculation at right angles from the center of the back surface of the circular lid body 31 in addition to the configuration of the lid 3 described above. This rod 34 is
When the cover 3A is covered with the cover 3A, the length thereof is set so that the tip of the cover 3A can be inserted to a position close to the cylinder main body bottom plate portion 24.

Further, the lid 3B with the vent hole is provided with a cylindrical portion 35.
And a partition plate portion 36 that vertically partitions a middle position of the cylindrical portion 35, and a plurality of ventilation holes 37 formed in the partition plate portion 36. The upper part of the cylindrical body 2 is inserted into the lower part of the cylindrical part 35, and the upper part of the cylindrical part 35 is filled with a cotton plug as described later.

FIG. 4 is an exploded perspective view and a sectional view showing an example of a culture vessel which is a component of the culture apparatus of this embodiment. The culture vessel 5 of this example is also made of plastic, and is composed of a vessel body 6 and a vessel lid 7 placed over the vessel body 6. The vessel body 2 of the culture vessel 1 having the above-described configuration is inserted into the vessel body 6 from above. A possible culture tube mounting recess 8 is formed.

The container main body 6 has a hexagonal support plate portion 61, and a ventilation tube portion 62 extends vertically upward at the center thereof, and extends through the support plate portion 61 by the ventilation tube portion 62. A ventilation hole 63 is defined. In addition, the support plate portion 61 has concentrically formed culture tube mounting recesses 8 at six locations.

The culture tube mounting recess 8 of the present embodiment includes a cylindrical portion 81 extending vertically upward from the support plate portion 61, and a bottomed cylindrical portion extending downward from the support plate portion 61 in a tapered state. 82. When the tube main body 2 of the culture tube 1 is inserted from above, the flange 23 rides on the upper edge of the cylindrical portion 81, and the periphery of the slitted lower portion 22 in the tube main body 2 is completely covered. Is formed.

The container lid 7 of the container body 6 is composed of a hexagonal lid plate portion 71 and a cylindrical portion 72 having a hexagonal cross section continuous with the outer peripheral edge. At the center of the lid plate portion 71, a ventilation cylinder portion 73 extending vertically upward is formed,
A ventilation hole 74 extending through the cover plate portion 71 is defined by the ventilation tube portion 73. In a state where the container lid 7 is put on the container main body 6, as shown in FIG. 4B, the outer peripheral edge of the lower end of the container lid 7 is in close contact with the support plate portion 61, whereby the culture tube mounting recess 8 is formed. The space on the upper opening side is covered.

Next, the culture tube 1 constructed as described above is used.
An example of a method for culturing Matsutake fungi for transplantation using a culture apparatus including the culture vessel 5 will be described.

First, as shown in FIG. 5, the tube main body 2 of each culture tube 1 is inserted into the culture tube mounting recess 8 of the culture container 5, the agar medium 11 is put therein, and the lid 3A is covered.
In this state, the entirety is sterilized or sterilized. At this time, the cotton plug 14 filling the vent 63 of the culture vessel 5 is also sterilized or sterilized at the same time. In addition to this, cotton plug 1
The lid 13B filled with 3 and the protective cylinder 4 are also sterilized or sterilized together. Similarly, the container lid 7 of the culture container 5
Sterilization or sterilization is performed in a state where the cotton plug 15 is filled in the ventilation hole 74. As a sterilization method, for example, an autoclave can be adopted.

Next, the seed inoculation cover 3A is pulled out,
A matsutake spore, a matsutake fruiting body tissue, etc., are attached to the tip of the rod 34, and the inoculum is inoculated on the agar medium 11 in each cylinder body 2.

Thereafter, as shown in FIG.
Then, a lid 3B with a vent hole that has been sterilized with the cotton plug 13 covered is put on.

Thereafter, the container lid 7 is put on the container body 6 and the culture container 5 is left for a predetermined period under predetermined atmosphere conditions and culture conditions. Generally, it is left for a predetermined period in an atmosphere of 23 degrees Celsius and 70% humidity. As a result,
The matsutake seed fungus 12 planted on the agar medium 11 of the tube main body 2 of each culture tube 1 proliferates, and the matsutake hypha grows, forming a state of extending outside from the slit 26 of the tube main body 2.
Here, sufficient oxygen is required for the growth of matsutake fungi, but in this example, a lid 3B with a vent is used as a lid for closing each agar medium 11, and a vent 6
3, 74 are formed, which are provided with breathable cotton plugs 13, 14, 15. Therefore, sufficient oxygen can be supplied to the matsutake fungi during the culture, and the invasion of various bacteria and the like from the outside can be reliably prevented.

Next, a procedure for transplanting the matsutake fungi for transplantation into an artificial medium or a natural medium after culture will be described. First, after the matsutake fungus has grown sufficiently, remove the lid of the culture vessel 5,
The culture tube 1 is removed from each culture tube mounting recess 8. Next, the lid 3B of each culture tube 1 is removed, and the lid 3 is covered instead, as shown in FIG. Further, the protection cylinder 4 is attached to a lower portion of the cylinder main body 2. As a result, the grown Matsutake fungi for transplantation is in a state of being covered by the protective tube 4.

The culture tube 1 is transported to, for example, a cultivation place where a large number of cultivation containers 17 on which an artificial medium 16 as shown in FIG. 9 is formed, and the tube main body 2 is taken out.
Embed in artificial medium 16. This completes the transplant operation.

Alternatively, the transplanting destination may be a portion (natural medium) where the fine roots near the ground surface at the root of the Japanese red pine 18 in the Japanese red pine forest as shown in FIG. In this case, for example, as shown in FIG. 8, the culture tube 1 is placed in a bag 19 with a seal, and is transported to a transplantation destination in this state, so that invasion of various bacteria can be reliably prevented. preferable.

[0038]

As described above, according to the present invention,
It is possible to easily inoculate the Matsutake inoculum on the solid medium, and it is possible to bring the tube body of the culture tube containing the cultured Matsutake fungus for transplantation as it is to the transplant destination and transplant it. Furthermore, the risk of infection with various bacteria and the like in the culture and transplantation work is extremely low.

[Brief description of the drawings]

FIG. 1 is a perspective view showing an example of a culture tube to which the present invention is applied.

FIG. 2 is an exploded perspective view of the culture tube of FIG. 1, which also shows three types of lids used for the culture tube.

FIG. 3 (a) is a cross-sectional view showing a culture tube covered with a seed inoculation cover, and FIG. 3 (b) is a cross-sectional view showing a state in which a cover with an air hole is covered on the tube main body of the culture tube.

FIG. 4 is a perspective view and a sectional view showing a culture vessel to which the present invention is applied.

FIG. 5 is an explanatory diagram illustrating a method for culturing Matsutake fungi for transplant to which the present invention is applied.

FIG. 6 is a cross-sectional view showing a state of matsutake fungi for transplantation to which the present invention is applied during culture.

FIG. 7 is an explanatory view showing a state in which a lid and a protective cylinder are put on the cylinder main body of the culture cylinder after culturing.

FIG. 8 is an explanatory diagram showing a state in which a culture tube after culture is stored in a bag.

FIG. 9 is an explanatory diagram showing a state in which Matsutake fungi for transplantation have been transplanted into an artificial medium.

FIG. 10 is an explanatory diagram showing a state where matsutake fungi for transplantation have been transplanted into a natural medium.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Culture tube 2 Tube main body 21 Upper part 22 Lower part 23 Flange 24 Bottom plate part 25 Outer peripheral wall part 26 Slit 27 Upper end opening 3, 3A, 3B Lid 31 Lid main part 32 Cylindrical part 33 Knob plate part 34 Rod 37 Vent hole 4 Protective tube 5 Culture container 6 Container main body 61 Support plate portion 63 Vent hole 7 Container cover 74 Vent hole 8 Culture tube mounting recess 11 Agar medium 12 Matsutake inoculum 13, 14, 15 Cotton plug 16 Artificial medium 17 Cultivation container 19 Cultivation container 19 bags

Claims (8)

[Claims] 1. A culture tube for use in a method of cultivating Matsutake fungi for transplantation, which comprises purely cultivating Matsutake species using a solid medium, wherein the culture tube is detachably mounted on a cylindrical body having a bottom and an upper end opening of the cylindrical body. A culture tube of Matsutake fungus for transplantation, comprising a lid and a slit formed in an outer peripheral wall of the tube main body. 2. The transplantation method according to claim 1, wherein the lid includes an inoculation seed inoculation lid as the lid, and the inoculation inoculation lid includes a rod that can be inserted into the cylinder main body. Matsutake fungus culture tube. 3. The culture tube for matsutake fungi for transplant according to claim 1, wherein the cover is provided with a cover having a vent hole as the cover. 4. The matsutake fungus for transplantation according to claim 1, further comprising a protective cylinder with a bottom, into which a slit-forming portion of the cylinder main body can be inserted. Culture tube. 5. A culture apparatus used for a method for culturing Matsutake fungi for transplantation, which comprises purely cultivating Matsutake fungi using a solid medium, comprising a culture vessel and at least one culture tube, wherein the culture vessel comprises: A culture tube mounting recess in which the culture tube can be inserted from above, a container body in which the culture tube mounting recess is formed, and a detachable cover on the opening side of the culture tube mounting recess in the container body. A container lid, a vent formed in the container main body, and a vent formed in the container lid, wherein the culture tube is attached to and detached from a tube main body having a bottom and an upper end opening of the tube main body. A matsutake fungus for transplantation, comprising: a cover capable of being covered; a slit formed in an outer peripheral wall of the cylinder main body; and a bottomed protective cylinder into which a slit forming portion of the cylinder main body can be inserted. Culture equipment. 6. A method for culturing Matsutake fungi for transplantation using the culture apparatus according to claim 5, wherein a tube main body of the culture tube is inserted into the culture tube mounting recess in the container body of the culture container. After that, a solid culture medium is put in the cylinder body, and a seed inoculation lid having a rod that can be inserted coaxially in the cylinder body is prepared as the lid that covers the cylinder body. Cover the cylinder body, sterilize in that state, pull out the seed inoculation lid, attach the matsutake inoculum to the rod, inoculate the matsutake inoculum at the rod tip on a solid medium, and cover the cylinder body. As a lid, a lid with a vent which is sterilized in a state where the vent is sealed with a cotton plug is prepared, and the lid is covered on the cylinder body. Then, the vent is closed with a cotton plug. Sterilized In this state, the culture container is left under a predetermined atmosphere temperature and humidity for a predetermined period to thereby obtain a solid culture medium in each culture tube. A method for culturing Matsutake fungi for transplantation, comprising: growing Matsutake hyphae on a vegetative path. 7. The culture tube in which matsutake hyphae have been grown by the method according to claim 6 is removed from the culture tube mounting recess of the culture container, and the culture tube is placed in the protection tube. And transferring the culture tube to a transplant destination. 8. The method for transplanting Matsutake fungi according to claim 7, wherein the culture tube is extracted from the protection tube at the transplantation destination and embedded in the transplantation medium.