JP2001278719A - Antiseptic, mildew-proofing and seaweed-proofing agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To develop an antiseptic, mildew-proofing and seaweed-proofing agent having both of antiseptic and mildew-proofing effects and seaweed-proofing effects, and capable of preventing emergence of various kinds of microorganisms by a small amount of the agent over a long period. SOLUTION: This antiseptic, mildew-proofing and seaweed-proofing agent is characterized in that the agent contains two kind of components of an oil of Melaleuca alternifolia, and an oil of Japanese cypress.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to an antiseptic and antifungal agent for water treatment of pools, fire protection equipment, fountains, leisure lands, etc., and an antiseptic and antifungal agent for water treatment used in the production process of industrial products. , Antiseptic and fungicidal agents to be used on floors, walls, ceilings, etc. in storage locations of industrial raw materials and products, product manufacturing locations,
The present invention relates to an antiseptic and antifungal agent for industrial raw materials and products, an antiseptic and antifungal agent for building structures, and an antiseptic and antifungal agent for household water.

[0002]

2. Description of the Related Art Preservatives and fungicides and antialgal agents are used to prevent various adverse effects (environmental pollution and deterioration by microorganisms) due to the growth and proliferation of bacteria, yeasts, molds and algae. Tea tree oil and lye oil, which are active ingredients of the antiseptic and antifungal agent of the present invention, are both known as antibacterial and antifungal agents.

[0003]

In order to prevent environmental pollution and deterioration by microorganisms, it is necessary to non-selectively and eradicate the generation of various microorganisms such as bacteria, yeasts, filamentous fungi and algae. . However, conventional antiseptics and fungicides and anti-algae agents are often much less effective or difficult to maintain their effects over a long period of time when their use is reduced in consideration of toxicity to humans and animals. Further, few of the conventional drugs sufficiently exhibit both the antiseptic and antifungal effects and the antialgal effect. Accordingly, development of a novel antiseptic and antifungal agent that has both antiseptic and antifungal effects and antialgal effects and can prevent the generation of various microorganisms for a long period of time with a small amount of medicine, in place of conventional agents, has been required. Requested.

[0004]

Means for Solving the Problems The present inventors have made intensive studies to solve the above-mentioned problems, and as a result, by using tea tree oil and hiba oil together, various microorganisms such as bacteria, yeasts, filamentous fungi and algae have been obtained. The present invention has been found to be useful as an antiseptic and fungicide for preventing environmental pollution and deterioration due to water. That is, the present invention relates to an antiseptic and antifungal agent comprising two kinds of active ingredients, tea tree oil and hiba oil.

[0005] The antiseptic and antifungal agent of the present invention exhibits a synergistic effect in the algal and antifungal effect while maintaining the antiseptic and antifungal effect as compared to the case where tea tree oil or lye oil is used alone.

As a result, particularly in a water storage facility or the like, since it exhibits a strong clean-keeping effect at a low dose, it is useful as an antiseptic and fungicide.

[0007] The preservative / mold / algae preventive means an agent capable of suppressing the generation of bacteria, mold and algae in a water storage facility or the like, and consequently keeping water clean for a long period of time.

[0008] In this case, the generation of algae cannot be suppressed only by a drug having an antiseptic / fungicidal action, and the occurrence of bacteria and mold cannot be suppressed by a drug having an antialgal action alone. It cannot be a fungicide.

It is considered that the antiseptic and antifungal agent basically needs to have both antiseptic and antifungal activities and antialgal effects. Any chemical can be used as long as it can keep the product clean.

[0010] The antiseptic and antifungal agents are not limited to water storage facilities,
It can be used in industrial facilities or products wherever water is present, such as in buildings and homes where water can flow, resulting in the development of bacteria, mold and algae.

[0011] Hiba oil means an essential oil obtained by steam distillation of waste material of Hiba (Japanese cypress asaro), which is a conifer of the genus Asunaro of the cypress family.

[0012] Tea tree oil means an essential oil obtained by steam distillation of the leaves of a tea tree (Melaleucaalternifolia), which is a tree of the family Myrtaceae.

[0013]

BEST MODE FOR CARRYING OUT THE INVENTION The preservative and fungicide of the present invention may contain tea tree oil and lye oil as active ingredients.

The antiseptic / fungicidal agent of the present invention can also be used as an antiseptic / antifungal agent or an antialgal agent.

The preparation of the antiseptic / fungicidal agent of the present invention is prepared by adding a suitable carrier and auxiliary agents such as a surfactant, a binder and a stabilizer to the active ingredient containing tea tree oil and coconut oil used in the present invention. These are mixed and mixed, and formulated into wettable powders, emulsions, liquids, sols (flowables) and other appropriate dosage forms by a conventional method.

In preparing these preparations, the amount of the active ingredient including tea tree oil and coconut oil is not particularly limited as long as wettable powders, emulsions, solutions, sols and other suitable preparations can be prepared. But not in the range of 1-90% by weight, preferably 10-80% by weight, based on the weight of these preparations.

As the carrier that can be used, any solid or liquid can be used as long as it is commonly used for industrial antibacterial and antifungal agents and antialgal agents, and it is not limited to a specific carrier.

Examples of solid carriers include mineral powders such as kaolin, bentonite, clay, montmorillonite, diatomaceous earth, mica, vermiculite, gypsum, calcium carbonate, phosphorous lime, white carbon, slaked lime, silica sand, ammonium sulfate, urea and the like, or Vegetable powders such as soybean flour, starch, crystalline cellulose, etc., alumina, silicates, sugar polymers, highly dispersible silicic acids, waxes and the like can be mentioned.

Examples of liquid carriers include water, alcohols such as methyl alcohol, ethyl alcohol, n-
Propyl alcohol, isopropyl alcohol, ethylene glycol, propylene glycol, benzyl alcohol and the like, aromatic hydrocarbons such as benzene, toluene, xylene, ethylbenzene, chlorobenzene, cumene, methylnaphthalene and the like, or halogenated hydrocarbons,
For example, chloroform, dichloromethane, ethylene dichloride and the like, ethers such as ethyl ether, dioxane, tetrahydrofuran and the like, ketones such as acetone, methyl ethyl ketone, cyclohexanone, methyl isobutyl ketone and the like, esters such as ethyl acetate, butyl acetate, ethylene glycol acetate, Amyl acetate and the like, nitriles such as acetonitrile, propionitrile, acrylonitrile and the like, sulfoxides such as dimethylsulfoxide and the like, alcohol ethers such as ethylene glycol monomethyl ether, ethylene glycol monoethyl ether and the like, amines such as triethylamine and the like, and Aliphatic and alicyclic hydrocarbons such as n-hexane and cyclohexane, and industrial gasoline (petroleum ether , Solvent naphtha, etc.), and petroleum fractions (paraffins, kerosene, gas oil, etc.), and the like.

Emulsion, wettable powder, sol (flowable),
For preparations such as liquids, emulsification, dispersion, solubilization, wetting,
A surfactant may be added for the purpose of spreading or the like.

Examples of such a surfactant include the following, but are not limited thereto.

Examples of the nonionic surfactant include polyoxyethylene alkyl ether, polyoxyethylene alkyl ester, polyoxyethylene sorbitan alkyl ester and sorbitan alkyl ester.

Examples of the anionic surfactant include alkyl benzene sulfonate, alkyl sulfosuccinate, alkyl sulfate, polyoxyethylene alkyl sulfate, aryl sulfonate and lauryl sulfate.

Examples of the cationic surfactant include alkylamines (laurylamine, stearyltrimethylammonium chloride, alkyldimethylbenzylammonium chloride and the like).

Examples of the amphoteric surfactant include carboxylic acid (betaine type) sulfate and the like.

In addition to these, polyvinyl alcohol (PVA), carboxymethyl cellulose (CM)
C), gum arabic, polyvinyl acetate, gelatin, casein, sodium alginate, tragacanth gum,
Thickeners such as guar gum, xanthan gum and hydroxypropylcellulose and various adjuvants can be blended.

If necessary, a suitable amount of a stabilizer such as an antioxidant or an ultraviolet absorber can be added.

If necessary, the antiseptic / fungicidal agent of the present invention may further contain other known antiseptic / fungicidal agents or antialgal agents.

Representative examples are listed below, but the present invention is not limited to these.

Quaternary ammonium compounds such as N, N-didecyl-N, N-dimethylammonium chloride and benzalkonium chloride; aldehyde compounds such as glutaraldehyde; 1,2-dibromo-2,4-dicyanobutane;
2,2-dibromo-3-nitrilopropionamide, 2
-Bromo-2-nitropropanediol, 5-bromo-
Halogenated compounds such as 5-nitro-1,3-dioxane; N-halogen compounds such as bromochlorodimethylhydantoin and trichloroisocyanuric acid; guanide compounds such as poly (hexamethylene biguanide) hydrochloride; zinc dimethyldithiocarbamate; Zinc diethyldithiocarbamate, zinc dibutyldithiocarbamate, zinc ethylphenyldithiocarbamate, zinc ethylenebisdithiocarbamate, zinc propylenebisdithiocarbamate, bis (dimethyldithiocarbamoyl)
Dithiocarbamate-based metal compounds such as zinc ethylenebisdithiocarbamate, manganese ethylenebisdithiocarbamate, nickel dimethyldithiocarbamate, nickel dibutyldithiocarbamate, copper dimethyldithiocarbamate, and iron dimethyldithiocarbamate; copper powder;
Copper-based metal powders such as copper-nickel alloy powder; copper such as cuprous oxide, cuprous thiocyanate (copper rhodan), basic copper carbonate, copper pyrophosphate, copper naphthenate, copper abietic acid, copper oxyquinoline Pyridion-based metal compounds such as zinc salt of 2-pyridinethiol-1-oxide and copper salt of 2-pyridinethiol-1-oxide; tetramethylthiuram disulfide, tetraethylthiuram disulfide, tetra-n-propylthiuram disulfide , Tetraisopropylthiuram disulfide, tetra-n-butylthiuram disulfide, tetraisobutylthiuram disulfide, N, N'-ethylenebisthiocarbamoylsulfide, N, N'-propylenebisthiocarbamoylsulfide, N, N'-butylene Conversion of thiurams such as bisthiocarbamoyl sulfide Compound; 2- (4-thiazolyl) benzimidazole, 2- (methoxycarbonylamino) benzimidazole, methyl-1- (ω-cyanopentylcarbamoyl) -2-benzimidazole,
Benzimidazole compounds such as zinc mercaptobenzimidazole and 2-thiocyanomethylthiobenzimidazole; 2-mercaptobenzothiazole,
(Thiocyanomethylthio) benzothiazole, 2- (thiocyanomethylsulfonyl) benzothiazole, 2-thiocyanoethylthio-4-chlorobenzothiazole, 2
-Thiocyanopropylthio-5,7-dichlorobenzothiazole, 2-thiocyanomethylthio-4,5,6,7
Benzothiazole compounds such as tetrachlorobenzothiazole; tetrafluoroisophthalonitrile, tetrachloroisophthalonitrile, 5-chloro-2,4-difluoro-6-methoxyisophthalonitrile, 2,4,4
Nitrile compounds such as 5,6-tetrachloroisophthalonitrile; 5-chloro-2-methyl-3-isothiazolone, 5-chloro-2-n-decyl-3-isothiazolone, 4,5-dichloro-2- ( 4-chlorobenzyl)-
3-isothiazolone, 4,5-dichloro-2- (4-chlorophenyl) -3-isothiazolone, 4,5-dichloro-2-n-hexyl-3-isothiazolone, 4,5-
Trimethylene-2-methyl-3-isothiazolone, 4-
Methyl-5-chloro-2-n-octyl-3-isothiazolone, 2-methyl-3-isothiazolone, 4,5-dichloro-2-n-octyl-4-isothiazoline-3-
Isothiazoline-based compounds such as on, 2-n-octyl-4-isothiazolin-3-one and 1,2-benzoisothiazolin-3-one; 1- [2- (2,4-dichlorophenyl) -4-propyl-1 , 3-Dioxolanyl-
2-methyl] -1H-1,2,4-triazole, 4,
Triazole compounds such as 4-dimethyl-2 (1,2,4-triazol-1-yl) -1- (4-trifluoromethyl-2-chlorophenyl) -1-penten-2-ol; 5,6-tetrachloro-4- (methylsulfonyl) pyridine, 2,3,6-trichloro-4
-Propylsulfonylpyridine, 2,6-dichloro-
Pyridine compounds such as 3,5-dicyano-4-phenylpyridine; 2,4-dichloro-6- (o-chloroanilino) -s-triazine, 2-chloro-4-methylamino-6-isopropylamino-s-triazine 2-chloro-4,6-bis (ethylamino) -s-triazine,
2-chloro-4,6-bis (isopropylamino) -s
-Triazine, 2-methylthio-4,6-bis (ethylamino) -s-triazine, 2-methylthio-4-ethylamino-6-isopropylamino-s-triazine,
Triazine-based compounds such as 2-methylthio-4-t-butylamino-6-cyclopropylamino-s-triazine; N- (3,4-dichlorophenyl) -N′-methylurea, 3- (3,4-dichlorophenyl) ) -1,1-Dimethylurea, 3- (3,4-dichlorophenyl) -1-
Methoxy-1-methylurea, 1- (α, α′-dimethylbenzyl) -3-methyl-3-phenylurea, 1- (2
-Methylcyclohexyl) -3-phenylurea, 3-
Urea compounds such as (4-isopropylphenyl) -1,1-dimethylurea; 2,3-dichloro-1,4-naphthoquinone, 2-amino-3-chloro-1,4-naphthoquinone, 2,3-dicyano Quinone compounds such as -1,4-dithiaanthraquinone; N-trichloromethylthiotetrahydrophthalimide, N-1,1,2,2-tetrachloroethylthiotetrahydrophthalimide, N-trichloromethylthiophthalimide, N-fluorodichloromethylthiophthalimide, N , N-Dimethyl-N'-phenyl-N '-(fluorodichloromethylthio) sulfamide, trichloromethylthiomethanesulfone-p-chloroanilide, N- (1,1,2,2, -tetrachloro-2-fluoroethylthio) ) Methanesulfonanilide,
N-fluorodichloromethylthio-N-3-chlorophenyl-N'-dimethylurea, N-fluorodichloromethylthio-N-3,4-dichlorophenyl-N'-methylurea, N-fluorodichloromethylthio-N-trissulfonyl-N N-haloalkylthio compounds such as -methylamine; N-phenethyldichloromaleimide;
Benzyldichloromaleimide, N- (2-chlorophenyl) maleimide, N- (4-fluorophenyl) maleimide, N- (3,5-dichlorophenyl) maleimide,
N- (2,4,6-trichlorophenyl) maleimide,
N-4-tolylmaleimide, N-2,4-xylylmaleimide, 2,3-dichloro-N- (2 ′, 6′-diethylphenyl) maleimide, 2,3-dichloro-N- (2 ′
-Methyl-6'-diethylphenyl) maleimide, 2,
3-dichloro-N- (2 ', 6'-dimethylphenyl) maleimide, 2,3-dichloro-N- (2', 4 ', 6'-
Maleimide compounds such as trimethylphenyl) maleimide; 3,5-dimethyl-tetrahydro-1,3,5,
2 (H) -thiadiazin-2-one, 3,3′-ethylenebis (tetrahydro-4,6-dimethyl-2H-1,
3,5-thiadiazin-2-one, 3,5-dimethyl-
2-thiotetrahydro-1,3,5-thiadiazine,
Thiadiazine-based compounds such as 3,5-dibenzyltetrahydro-1,3,5-thiadiazine-2-thione; methyl thiocyanate, chloromethyl thiocyanate, ethyl thiocyanate, methylenebisthiocyanate, chloromethylenebisthiocyanate, ethylenebisthiocyanate Chloroethylene bisthiocyanate, isobornyl thiocyanate, methyl isothiocyanate, allyl isothiocyanate, phenyl isothiocyanate,
Thiocyan compounds such as benzyl isothiocyanate;
Alkyl phenol compounds such as capryl phenol and nonyl phenol; tris (octyl phenyl) phosphite, tris (nonyl phenyl) phosphite, tris (dinonyl phenyl) phosphite, tris (mono,
Alkyl phenyl phosphite compounds such as di-mixed nonylphenyl) phosphite; alkyls such as tris (octyl phenyl) phosphate, tris (nonyl phenyl) phosphate, tris (dinonyl phenyl) phosphate and tris (mono, di-mixed nonyl phenyl) phosphate Phenyl phosphate compound; O-3-iodo-2-propynyl-N-butyl carbamate, N-3-
Iodo-2-propynyl-O-butyl carbamate,
Iodine compounds such as 2,3,3-triiodoallyl alcohol and diiodomethylparatolylsulfone; polysulfide compounds such as n-octylchloromethyl disulfide; boron compounds such as pyridinetriphenylborane; phenyl (bispyridyl) bismuth dichloride and the like Bismuth compounds.

The antiseptic / fungus / algae preventive agent of the present invention containing tea tree oil and hiba oil as active ingredients can be used for the following applications.

1) Antiseptic, fungus- and algae-proof for water treatment of pools, fire prevention equipment, fountains, leisure lands and the like.

2) Antiseptic and antifungal algae for water treatment used in the process of building air conditioning and manufacturing industrial products, for example, preservation of cutting oil; cooling equipment in factory manufacturing facilities and building air conditioning; pulp and paper mills, etc. Sterilization and prevention of slime formation; protection of slime deposits on sugarcane and sugar beet sugar production equipment; prevention of microbial accumulation and deposition in air washers, scrubber systems and industrial freshwater supply systems; oilfield cutting oil, muddy water and secondary Prevent microbial contamination and sedimentation in oil recovery processes; Prevent microbial accumulation in photographic processing.

3) Antiseptic and antifungal algae used on floors, walls, ceilings, etc. of industrial raw materials and product storage locations, product manufacturing locations, etc., for example, paint films, especially paint films of exterior paints, are exposed to the weather. Protection from attacks by bacteria, fungi and algae that occur during the treatment; maintenance of sanitary environment in food factories; deodorization and sterilization of manufacturing facilities, sewage treatment plants, human waste treatment plants, etc.

4) Preservative and fungicide of industrial raw materials and products, such as aqueous paints, adhesives, emulsion products such as latex and acrylic, starch products, pigment products, slurry products such as calcium carbonate, and joint cement. Suppression of growth of bacteria, fungi and algae in the forest; Preservation of wood of building materials (building materials, civil engineering materials, etc.); Fungus protection of surfactants; Antibacterial and antifungal treatment by spraying or dipping fibers, fabrics and leathers; Interior / exterior materials (for housing and medical facilities), building materials (building materials, civil engineering materials, etc.), home appliances, household goods made of resins such as vinyl, polyurethane, polyethylene, polypropylene, silicone, modified silicone, nylon, and epoxy Antibacterial and antifungal and anti-algae for sports equipment, etc .; prevention of growth of bacteria and fungi in paper coating materials and coating processing; cosmetics and toiletries Prevention of microorganism contamination of over products; agricultural formulations, electrodeposition systems, prevention of diagnostic and pharmaceutical products, microbial contamination, such as medical equipment.

5) Antiseptic, mold, and algae for building structures.

6) Antiseptic and antifungal algae around water in homes, for example, antiseptic and antifungal algae in sinks, toilets, bathrooms, washrooms, etc .;

Tea tree oil is commercially available (Main Cam)
p Company Australian tea oil, etc.) can be used as it is.

As the hiba oil, a commercially available product (Aomori Prefectural Forestry Association Aomori Hiba Oil etc.) can be used as it is.

The mixing ratio (weight ratio) of tea tree oil and hiba oil is 0.1 to 10 (tea tree oil / hiba oil), preferably 0.5 to 2 (tea tree oil / hiba oil).
(Hiba oil).

Formulation examples in the case of using an active ingredient containing tea tree oil and coconut oil as an antiseptic / fungicide / algae preventive agent are shown below. Is not limited to these.

[0042]

Table 1 Formulation Example 1 (Liquid) Ingredients% by weight Active ingredient (tea tree oil + coconut oil) 60 Distilled water 40 100 The above was mixed and dissolved to obtain a liquid containing 60% of the active ingredient.

[0043]

Table 2 Formulation Example 2 (Liquid) Ingredients% by weight Active ingredient (tea tree oil + coconut oil) 60 Propylene glycol 7.5 Distilled water 32.5 $ 100 Was obtained.

[0044]

Table 3 Formulation Example 3 (Emulsion) Ingredient% by weight Active ingredient (tea tree oil + coconut oil) 30 Distilled water 20 Dimethyl sulfoxide 45 Methyl isobutyl ketone 2.5 Solpol 800A 2.5 (Emulsifier manufactured by Toho Chemical Co., Ltd.) # 100 The above were mixed and dissolved to obtain an emulsion containing 30% of the active ingredient.

[0045]

Table 4 Formulation Example 4 (Wettable powder) Ingredient% by weight Active ingredient (tea tree oil + coconut oil) 20 Distilled water 12 Lauryl sulfate 5 Clay 63 100 The above is uniformly mixed and pulverized. A wettable powder containing 20% was obtained.

[0046]

Table 5 Formulation Example 5 (Flowable agent) Ingredient% by weight Active ingredient (tea tree oil + coconut oil) 30 Lauryl sulfate 2 Xanthan gum 2 Hydroxypropylcellulose 1 Distilled water 65 100 Put the above in a ball mill for 12 hours The mixture was ground and mixed to obtain a flowable agent containing 30% of the active ingredient.

The formulated antiseptic / fungal / algae preventive agent of the present invention can be applied as various preparations as they are, or after dilution with water or a suitable organic solvent.

When used as an antiseptic and fungicidal agent for industrial raw materials and industrial products, they can be added to or mixed with various industrial raw materials or products, or applied to the surface of various industrial raw materials or products. Spraying methods or methods of immersing various industrial raw materials and products in a diluting solution of the industrial antiseptic and antifungal agent of the present invention, and the like, It can be used by various methods according to the method of using the antifungal agent and the algicide, but it is not limited to any particular method.

[0049]

EXAMPLES The present invention will be described below in more detail with reference to examples using the compounds of the present invention. However, the present invention is not limited to these examples.

Example 1 (Evaluation of antibacterial and antifungal activities) Solution 1: Tea tree oil Solution 2: Hiba oil Solution 3: Hiba oil / Tea tree oil = 2/1 Solution 4: Hiba oil / Tea tree oil = 1 / 1 Solution 5: Lark oil / Tea tree oil = 1/2 Each of the above solutions was diluted with dimethyl sulfoxide,
Solutions of various concentrations were prepared. For the bacteria, sensitization medium-N (Nissui Pharmaceutical) was used, and for the fungi, potato dextrose agar medium (Nissui Pharmaceutical) was used.
0.5 ml of the above solution was added to 5 ml and mixed, and the mixture was poured into a petri dish to form a solidified plate. The inoculated bacteria were cultured in a broth for sensitivity measurement (Nissui Pharmaceutical Co., Ltd.) at 37 ° C. for 20 hours, and the fungi were cultured in potato dextrose agar medium (Nissui Pharmaceutical Co., Ltd.) for 10 days, each having a 10 ⁶ CFU / ml suspension. Was prepared. The test bacterial suspension was streaked onto a drug-mixed agar plate using a platinum loop, and the bacteria were incubated at 37 ± 1 ° C. for 18-20 minutes.
Time and fungi were cultured at 27 ° C for 7 days, and the minimum growth inhibitory concentration (MI
C).

The results are shown in Table 1. However, the symbols in the table mean the following. A: Bacillus subtilis B: Escherichia coli C: Rhodotorula muciginosa
laginosa) D: Trichophyton mentagrophytes
yton mentagrophytes)

[0052]

Table 1 Activity against bacteria and fungi 溶液 Solution MIC (ppm) ABCD ───────────────────────── 1 1560 1560 3130 1560 2 100 1560 780 50 3 200 1560 780 50 4 390 3130 780 50 5 390 3130 1560 100 溶液 Solutions 3-5 consist of Solution 1 (tea tree oil) and Solution 2 (Hiba oil). Since it is a mixed solution of 2/1 to 1/2, its antibacterial and antifungal activity is expected to be stronger than solution 1 and weaker than solution 2. The actual activity showed an intermediate activity between the activity of the solution 1 and the activity of the solution 2 almost as expected, taking into account measurement errors and the like. Therefore, it was clarified that the antibacterial and antifungal activities were maintained (additive action) by mixing tea tree oil and cypress oil.

Example 2 (Evaluation of growth inhibitory activity against freshwater green algae) Solutions 1 to 5 prepared in Example 1 were each diluted with dimethyl sulfoxide to prepare solutions having various concentrations. Each of the above solutions was added to a medium containing 10 ⁵ / ml of freshwater green algae (Selenastrum capricornutum) in the logarithmic growth phase to prepare samples of various concentrations, and the mixture was allowed to stand at 23 ± 1 ° C for 24 hours under continuous lighting conditions. Cultured. After 72 hours, the number of cells was measured using a hemocytometer to show the growth inhibitory activity as EC ₉₀ (concentration that inhibits proliferation by 90%). The results are shown in Table 2.

[0054]

Table 2 Activity against freshwater green algae ──────────────── Solution growth inhibitory concentration (ppb) EC ₉₀ ──────────────── 1 160 2 720 3 86 4 60 5 65 溶液 Solutions 3 to 5 Since it is a mixed solution of 2/1 to 1/2 of Solution 1 (tea tree oil) and Solution 2 (Hiba oil), its growth inhibitory activity is expected to be stronger than Solution 2 and weaker than Solution 1. However, the actual activity was stronger than that of solution 1 in all of solutions 3 to 5. Therefore,
It was clarified that by mixing tea tree oil and cypress oil, the growth inhibitory activity was enhanced (synergistic action).

[0055]

Industrial Applicability The composition containing tea tree oil and hiba oil is highly safe, exhibits a wide spectrum at a low dose, and is useful as a preservative and fungicide.

Claims (3)

[Claims] 1. An antiseptic and antifungal agent comprising two kinds of active ingredients, tea tree oil and hiba oil. 2. The antiseptic / fungicidal agent according to claim 1, wherein the weight ratio of tea tree oil to hiba oil (tea tree oil / hiba oil) is in the range of 0.1 to 10. 3. The antiseptic and antifungal agent according to claim 2, wherein the weight ratio of tea tree oil to lime oil (tea tree oil / lime oil) is in the range of 0.5 to 2.