JP2001238608A - Feed for poultry farming - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a feed for poultry farming that can reduce bacterial infection, for example, salmonellosis. SOLUTION: The objective feed for poultry farming characteristically comprises a mannose-including powder and an attenuated vaccine.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a poultry feed for reducing bacterial contamination such as Salmonella in feed and bacterial infection such as Salmonella in chickens.

[0002]

BACKGROUND OF THE INVENTION Since 1989, food poisoning caused by Salmonella enteritides (SE) has rapidly increased, and contamination of Salmonella has spread to the poultry industry. Conventionally, an inverse disinfectant or the like has been used for disinfection of poultry houses, but at present there is no drastic solution. Overseas, vaccines are used. In Japan, the vaccine has been approved since April 1998, but it has not yet reached the point of preventing intestinal Salmonella infection. Japanese Patent Application Laid-Open No. 10-215790 reports on poultry feeds and the like to which heat treatment, a probiotic agent, an oligosaccharide and an acidifying agent are added. Furthermore, the ingestion of mannose-containing copra meal inhibits the intestinal adhesion of Salmonella chickens, WO99 / 08544
No. is reported in the official gazette.

[0003]

However, there is a problem that the vaccine against Salmonella inhibits the transfer of Salmonella from the intestine of the chicken to the body, but cannot prevent the colonization of Salmonella in the intestine. JP-A-10-21579
In the example of the No. 0 report, mannan oligosaccharide was added to the basal feed at a concentration of 0.
There is a problem that if added at a high concentration, diarrhea may occur and it is economically expensive. Furthermore, in the example of WO99 / 08544, 1% by mass of mannose-containing copra meal was added to the basal feed, but the number of salmonella in feces was 1 on day 6 after administration of Salmonella compared to day 0. However, there is a problem that the cost is only reduced to / 10 and the cost is high. Therefore, an object of the present invention is to provide a poultry feed that reduces bacterial contamination of salmonella and the like in feed and bacterial infection of salmonella and the like of laying hens and broilers that ingested the feed with a small amount of added concentration. It is.

[0004]

Means for Solving the Problems The present inventors have conducted intensive studies on various additives and the like in order to solve such problems, and as a result, the mannose-containing powder and the probiotic agent were simultaneously added to poultry feed. The present inventors have found that the effect of salmonella eradication is enhanced by the synergistic effect of the addition as compared with the case where it is added alone, and it has been found that the above-mentioned problem can be solved, and the present invention has been completed. That is, the gist of the present invention is a feed for poultry farming, characterized by adding a mannose-containing powder and a probiotic agent.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail.
In modern poultry farms, chicks are bred separately from the parent chicken and the normal intestinal flora of the parent chicken can not be directly ingested through feces, so the intestinal bacteria of the chick are undeveloped for two weeks after hatching. Yes, it is susceptible to attack by harmful bacteria such as Salmonella. Therefore, lactic acid bacteria, butyric acid bacteria, bifidobacteria, and CE (Comp
It has been reported that the addition of live bacterial agents such as etitive Exclusion preparations to feeds provides useful bacteria to chickens and inhibits colonization of harmful bacteria. The probiotic agents used in the present invention include lactic acid bacteria, butyric acid bacteria, bifidobacteria,
Examples of CE preparations include, for example, avigard (Bayer Co., Ltd.), inteclean (Itochu Feed Co., Ltd.), broilact (Fujisawa Pharmaceutical Co., Ltd.), Delro-
TE29 (Kyoritsu Shoji Co., Ltd.). When added at the same time as the mannose-containing powder, the concentration of the probiotic agent added to the feed should be 10
What is necessary is just to add 50 mass%-50 mass%.

The mannose-containing powder used in the present invention is a mannose, galactomannan, or glucomannan-containing substance such as copra meal, palm kernel meal, guar gum, locust bean gum, konjac powder or the like, which is treated with hemicellulase. Copra meal is a pulverized residue obtained by extracting coconut oil from copra, which is a coconut oil raw material obtained by drying core meat inside coconut fruit, and usually contains about 28% by mass of mannan. Palm kernel meal is a crushed residue of palm kernel oil extracted from palm kernel of oil palm seeds (Palm Kernel), and usually contains about 40% by mass of mannan. Guar gum, locust bean gum, and konjac powder are used as food ingredients and thickeners.

[0007] Hemicellulase is an enzyme that acts on hemicellulose, a polysaccharide that is present in plant cell walls by binding to cellulose, and that acts on substances containing mannan, galactomannan, and glucomannan to release mannose. It is not particularly limited as long as it is a mannanase (mannanase) and a mannan degrading enzyme such as mannosidase. Examples of the origin of such enzymes include Bacillus subtilis and filamentous fungi (Aspergillus aculeatus, A. et al.
awamori, A. et al. niger, A .; usamii,
Humicola insolens, Trichod
erma harzianum, T .; koningi,
T. longibrachiatum, T .; virid
e), Basidiomycetes (Corticium, Pycnopor)
us coccineus), and Asp.
Preferred are enzymes from ergillus. Among them, mannanase derived from Aspergillus niger is particularly preferred.

[0008] These hemicellulases are produced in the culture supernatant or cells of the above strains, and any fraction containing these hemicellulases may be used. If necessary, the fraction containing these hemicellulases may be purified or partially purified by a conventional method. Cellulosin HC100, cellulosin HC, cellulosin TP25, cellulosin GM5
Commercial enzymes such as Sumiteam AC, Sumiteam AC-L, Sumiteam ACH (Shin Nippon Chemical Co., Ltd.) and Gamanase (Novo Nordisk Industries) can also be used. it can. When the mannose-containing powder is added to the feed at a concentration of 0.001-2,
% By mass, preferably 0.005 to 1% by mass, and a mannose concentration of 0.0001 to 0.2% by mass.
Preferably, the content is 0.0005 to 0.1% by mass.

[0009]

The present invention will be described below in detail with reference to examples. Note that the present invention is not limited to these examples. Reference Example 1 Preparation of powder containing mannose Cellulosin GM5 (mannanase manufactured by Hankyu Bio-Industry Co., Ltd., titer 10,000 units / g)
3 g is suspended in 100 mL of water, and 100 g of copra meal is suspended.
(Fat content: 10% by mass, water content: 7.2% by mass), and then left at 60 ° C. for 18 hours. After completion of the reaction, a vacuum dryer (Vaccum Dryin, manufactured by Yamato Corporation)
g Oven DP32) under vacuum at 80 ° C for 24 hours to obtain a mannose-containing powder. The analysis of the sugar component in the target product was performed by high performance liquid column chromatography. Analytical column is Aminex HP manufactured by Bio-Rad
X-87P was used. Column temperature 85 ° C, flow rate 0.6
It was eluted with water at a rate of ml / min. The sugar was detected using a differential refractometer, and the mannose content was determined from the quantitative value of the standard product. As a result of analyzing the powder after the above reaction, 1
13 g of mannose had accumulated in 00 g. The water content was 12.0% by mass.

Example 1 Twenty five-week-old white-legged egg laying hens (Julia) were mixed with 0.02% by mass of the mannose-containing powder prepared in Reference Example 1 in a compound feed having the composition shown in Table 1. 0.1 kg per bird per day for 25 days with a mixed feed supplemented with 0.002% by mass of a probiotic agent (CE preparation, trade name: Avigad, Bayer)
(Or 2.5 kg of total donation).

[0011]

[Table 1]

On the 18th day after feeding, Salmonella bacteria (Salmonella Ent.
eritidis wild strain) was forcibly orally administered via a catheter with 1 ml of a bacterial solution containing 8.0 × 10 ⁵ cells / ml. 14 days after feeding (control), 1 day, 3 days and 7 days after Salmonella administration
Cecal feces discharged on the morning of the day were collected for each individual, and the number of Salmonella was measured as follows.

(Measurement method of Salmonella number) 1 g of cecal feces was diluted 10-fold with sterile phosphate buffered saline and mixed well to prepare a sample stock solution. Next, the sample stock solution was serially diluted with a sterile physiological saline solution at a common ratio of 10 to give a 100-fold diluted solution and 1
A 000-fold dilution was prepared. 0.1 ml each of the sample stock solution, 100-fold dilution and 1000-fold dilution was spread on SS agar plate medium and brilliant green agar plate medium, cultured at 37 ° C. for 24 hours, and grown on each plate medium. The target settlement was measured. Furthermore, the bacteria were collected from the colonies and used for lysine decarboxylation test, SIM agar medium and TSI agar medium (Cliggra
Inoculated in a modified medium for intestinal bacteria) and cultured at 37 ° C. for 24 hours to confirm the properties. If the colony is found to be Salmonella, the serotype is determined using Salmonella immune serum. Was confirmed, and the number of colonies recognized as the Salmonella O9 group was multiplied by the dilution ratio of the sample stock solution or diluent to calculate the number of Salmonella bacteria per gram of feces.

Comparative Examples 1 and 2 Also, for comparison, the above-mentioned mannose-containing powder 0.02 was used.
% Of mannose-containing powder (Comparative Example 1) instead of 0.002% by mass and
Salmonella eradication test was similarly performed using two kinds of feeds to which only 02% by mass (Comparative Example 2) was added.

FIG. 1 shows the results of Example 1 and Comparative Examples 1 and 2. In the figure, A is a chicken to which the feed of the present invention is added (Example 1), B is a chicken to which only 0.02% by mass of the mannose-containing powder is added (Comparative Example 1), and C is a live bacteria agent 0.002. The number of Salmonella in the cecal feces of the chicken (Comparative Example 2) to which only% by mass was added is shown. FIG. 1 is a diagram showing the results of a salmonella eradication test in chickens, in which the vertical axis represents the logarithmic value of the number of salmonella eradications, and the horizontal axis represents the number of days after Salmonella administration. From the above results, it can be seen that the feed to which the mannose-containing powder and the probiotic agent of the present invention are added has a better Salmonella eradication effect than when it is added alone.

[0016]

EFFECTS OF THE INVENTION The poultry feed of the present invention not only has the effect of reducing bacterial infections such as Salmonella, but also has the effect of inhibiting adhesion of Escherichia coli having type 1 pili to the intestinal tract and preventing Escherichia coli disease. .

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[Procedure amendment]

[Submission Date] March 14, 2000 (200.3.1)
4)

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] Brief description of drawings

[Correction method] Change

[Correction contents]

[Brief description of the drawings]

FIG. 1 is a diagram showing the results of a salmonella bacillus test in chickens.

[Description of Signs] A Chicken to which the feed of the present invention was added (Example 1) B Chicken to which only mannose-containing powder was added (Comparative Example 1) C Chicken to which only a probiotic agent was added (Comparative Example 2)

Claims (1)

[Claims] 1. A poultry feed to which a mannose-containing powder and a probiotic agent have been added.