JP2001145464A - Additive for feed of livestock, poultry and pet food - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an additive for feeds capable of enhancing palatability by mixing in feeds for livestock, poultry and pet foods and accelerating growth by increasing an amount of intake. SOLUTION: An additive for feeds capable of promoting palatability and accelerating growth by increasing the amount of intake is provided by blending a lipase decomposition product and a protease decomposition product of milk or a milk processing product in the feeds for livestock such as pigs, poultry such as chickens and pet foods for dogs, cats and the likes.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a feed additive for livestock, poultry and pet foods, and more particularly, the present invention contains as an active ingredient an enzymatically decomposed product of lipase and protease of milk or dairy products. Incorporating feed additives for livestock, poultry and pet food, which are characterized in that they are incorporated into livestock such as cattle and pigs, poultry such as chickens and feed for pet foods such as dogs and cats, thereby improving palatability and ingesting. The present invention relates to a feed additive capable of promoting growth by increasing the amount.

[0002]

2. Description of the Related Art Heretofore, it has been practically used to improve taste and increase food intake by adding additives such as flavors to livestock feed. For example, as a measure to speed the feeding of infant formula, the addition of a flavor with the aroma of breast milk facilitates the early weaning of suckling piglets, and the addition of sugar and sodium glutamate to the pigs. It has been known from an early stage that the palatability of rice is improved (Feedstuffs Vol. 33, 48, (Dec. 2) 61, 1961).

[0003] It has also been practiced to improve the palatability of livestock and the like by using processed milk products as an additive for feed, and to promote the growth of such products. A feed additive characterized by adding 0.1 to 1% by weight of a nucleic acid having an activating effect (JP-A-48-39251).
No. 5), a milk replacer composition for a calf characterized by containing skim milk powder and / or soybean meal as a main component and containing 0.5% by weight or more of a medium-chain fatty acid having 6 to 10 carbon atoms or a salt thereof. Product (Japanese Patent Laid-Open No. 2-261349) and 30% by weight of a semi-moist animal feed containing milk protein as a main component.
The semi-moist granular feed for cattle, pigs or experimental animals for young animals, characterized by comprising the above, having a water content of 20 to 30% by weight and a water activity of 0.93 or less No. 58-9654), skim milk powder as a main component, and 2.24% by weight or more of calcium gluconate based on the skim milk powder, and the remainder is dried whey, oils and fats,
A milk substitute composition for calves, comprising nutrient materials such as sugars, animal and plant raw materials, vitamins and inorganic substances (Japanese Patent Laid-Open No.
No. 7-186445), an animal feed additive comprising fermented milk (Japanese Patent Application Laid-Open No. 60-141232), butter obtained by centrifuging milk, and butter obtained by churning, or an oil-soluble fraction thereof. Animal feed for pets to which a water-soluble fraction or a water-soluble fraction is added (JP-A-63-188350)
Publication).

[0004] However, the use of processed dairy products can improve palatability. However, in general, processed milk products are expensive, so that the added amount is suppressed, and if the added amount is suppressed, the palatability is reduced. There was a problem. Therefore, in order to solve this difficulty, regarding the use of an enzymatically decomposed product of milk protein by a protease or an enzymatically decomposed product of milk fat by a lipase as a feed additive, for example, a casein decomposed product containing casein phosphopeptide as a main component Feed for pigs, characterized by containing (JP-A-5-2688)
No. 83), a liquid feed composition for ruminant animals, comprising a protein decomposed product and / or an amino acid together with a saccharide and a medium-chain fatty acid triglyceride, and having an osmotic pressure adjusted to 200 to 450 mOsm / liter. A lipolytic enzyme is added to a product (JP-A-6-133702), milk and whey, or an agent for preventing and treating infectious diseases of fish and crustaceans containing milk whey hydrolyzate as an active ingredient (JP-A-9-315983) and butter. Chicken and egg flavor improving agent containing butter flavor and matsusoyalactone obtained by decomposition as active ingredients (Japanese Patent No. 2739376), a liquid phase portion of a hydrolyzed product of shrimp having head and chest, and milk fat water A palatability improver for piglet feeds, characterized by containing a decomposition product as an active ingredient.
No. -4172) has been proposed.

[0005]

However, the use of the above-mentioned enzymatically decomposed product of milk protein by protease or milk fat by lipase as an additive for feed can improve the palatability to some extent. Was not always satisfactory.

Accordingly, an object of the present invention is to improve palatability and increase intake by blending in feed for livestock such as cows and pigs, poultry such as chickens, and pet food such as dogs and cats. The purpose of the present invention is to provide a feed additive capable of promoting growth.

[0007]

Means for Solving the Problems The present inventors have conducted intensive studies in order to solve the above-mentioned problems, and as a result, a mixture of enzymatically decomposed products of milk or processed milk products by lipase and protease has been used in cattle, pigs, etc. The present invention has been found to be able to improve the palatability by adding it to feed additives for domestic animals, poultry such as chickens, and pet foods such as dogs and cats, and to promote growth by increasing the intake amount. Was completed.

Thus, according to the present invention, there is provided a feed additive for livestock, poultry and pet foods, which contains, as an active ingredient, enzymatically degraded products of milk or processed milk products by lipase and protease.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail.

The milk or processed milk product which can be used in the present invention includes, for example, milk such as raw milk, ordinary milk and processed milk; creams such as cream and fat-substituted cream; skimmed milk powder, whole milk powder and the like Powdered milk; condensed milk such as sugar-free condensed milk and sweetened condensed milk; cheeses, butter and buttermilk;

The enzymatic degradation product of milk or processed milk product by lipase and protease used in the feed additive of the present invention is a decomposed product prepared separately from a lipase degradation product and a protease degradation product; And a degradation product obtained by simultaneously treating with milk and a protease; a degradation product obtained by treating milk or a processed milk product with a lipase and then treating with a protease; and a degradation product obtained by treating a milk or processed milk product with a protease and then treating with a lipase. Both can be used.

The lipase that can be used in the present invention is not particularly limited.
Lipase collected from various microorganisms such as Aspergillus, Mucor, Rhizopus, Penicillium, Candida, Pichia, Chromobacterium, lipase obtained from pig pancreas, pups, lambs, calves Oral lipase or the like collected from the oral secretory line can be appropriately used. These lipases can be used alone or in combination.

The protease that can be used in the present invention is not particularly limited. For example, various microorganisms such as Aspergillus, Mucor, Rhizopus, Streptococcus, Lactobacillus, Penicillium, Bacillus, etc. Proteases such as bromelain and papain which can be collected from plants, and proteases such as trypsin and pepsin collected from animal organs. These proteases can be used alone or in combination of several kinds.

The enzymatic treatment of milk or dairy products using the lipase is carried out by disinfecting the above-mentioned homogeneous mixture of milk or dairy products and water, for example, at 65 to 85 ° C. for 10 to 30 minutes. After cooling, for example, 0.005 to 5% by weight of a lipase such as a microbial lipase or an oral lipase is added, and the mixture is stirred or allowed to stand at 25 ° C. to 55 ° C. for 8 hours to 48 hours. It can be obtained by enzymatic treatment. It is preferable that the lipase decomposes the lipid of milk or processed milk products (acid value / saponification value × 100) to about 20 to 75%. When a decomposition product having a decomposition rate outside this range is used for feed additives for livestock, poultry, and pet food, palatability is poor and not good. After completion of the enzymatic decomposition, the pH of the degraded product is adjusted, if necessary, and the enzyme is inactivated by heating, whereby an enzymatically degraded product of milk or processed milk products by lipase can be obtained.

In the present invention, the above-mentioned lipase hydrolyzate can be used as it is, but it is generally preferable to concentrate the hydrolyzate and use it as a concentrate. Furthermore,
The degraded product may be separated into lipase-decomposed products by separating an oil portion using an appropriate separation means such as centrifugation. Still further, the degraded product may be used in the form of a composition with a suitable diluent or carrier. Such diluents or carriers include, for example, solid diluents or carriers such as gum arabic, dextrin, glucose, sucrose, or liquid diluents or carriers such as water, ethanol, propylene glycol, glycerin, and surfactants. The lipase hydrolyzate of the above-mentioned milk or dairy product can be exemplified by using any of these diluents or carriers in any dosage form, for example, powder, granule, liquid, emulsion, paste, or any other appropriate form. Can be prepared in the form of a powder or granule, for example, by adding gum arabic, dextrin or the like to obtain a powder or granule from the viewpoint of stability.

The enzymatic treatment of milk or dairy products using the above protease is carried out by sterilizing the above-mentioned homogeneous mixture of milk or dairy products with water, for example, at 65 ° C. to 85 ° C. for 10 minutes to 30 minutes. After cooling, a protease such as a microbial protease, a plant protease, or an animal protease is added in an amount of 0.005 to 5% by weight.
By enzymatic treatment under stirring or standing conditions for 8 hours to 48 hours. After completion of the enzymatic decomposition, the pH of the decomposed product is adjusted, if necessary, and the enzyme is deactivated by heating, so that an enzymatically decomposed product of milk or a processed milk product can be obtained.

In the present invention, the above-mentioned protease hydrolyzate can be used as it is, but it is generally preferable to concentrate the hydrolyzate and use it as a concentrate. Further, the decomposed product may be used in the form of a composition with a suitable diluent or carrier. Such diluents or carriers include, for example, solid diluents or carriers such as gum arabic, dextrin, glucose, sucrose,
Alternatively, a liquid diluent or carrier such as water, ethanol, propylene glycol, glycerin, and a surfactant can be exemplified, and the above-mentioned protease degradation product of milk or dairy products can be prepared using any of these diluents or carriers. Dosage form, for example, powder, granule, liquid, emulsion, paste, and other appropriate dosage forms can be prepared, for example, powder or granule by adding gum arabic, dextrin, etc. Is preferred in terms of stability.

The lipase hydrolyzate and the protease hydrolyzate of the above-mentioned milk or processed milk product can be mixed in advance to prepare the feed additive of the present invention, or they can be separately added to the feed. The blending ratio of the lipase hydrolyzate and the protease hydrolyzate is not particularly limited. For example, 0.1 part by weight to 10 parts by weight of the protease hydrolyzate can be exemplified with respect to 1 part by weight of the lipase hydrolyzate.

In the present invention, the enzymatic degradation of the above-mentioned milk or processed milk product by lipase and the enzymatic degradation by protease can be carried out simultaneously. For example, after the milk or processed milk product is degraded by lipase, it can be digested with protease. Any method of decomposing, or for example, a method of decomposing milk or a processed milk product with a protease and then decomposing with lipase can be employed.

Further, if desired, the feed additive for livestock, poultry and pet food of the present invention may contain, for example, amino acids, vitamins, feed flavors and the like which are generally known as feed additives. Livestock, poultry, feed additives for pet food of the present invention obtained in this manner, in a compound feed for livestock produced in large quantities, for example,
0.01 to 1% by weight, preferably 0.05 to 0.2% by weight based on the mixed feed. Cattle, livestock such as pigs, poultry such as chickens and dogs,
By blending it in feed for pet foods such as cats, it is possible to improve palatability, and to increase the intake to promote growth.

[0021]

Next, the present invention will be described more specifically with reference to examples and reference examples.

Reference Example 1 (Preparation of lipase hydrolyzate of milk or processed milk product) 200 g of whole milk powder was dissolved in 750 g of water with stirring, and the mixture was heated to about 75 ° C.
After cooling at 30 ° C., 0.2 g of Lipase AY (trade name of Lipase manufactured by Amano Pharmaceutical Co., Ltd.) is added to 50 g of water.
Was added and the mixture was subjected to enzymatic degradation at 30 ° C. to 35 ° C. for 24 hours. At this time, the decomposition rate of the lipid (acid value / saponification value × 100) was 50%. After enzymatic degradation, about 85
C. for 15 minutes to inactivate the enzyme. After cooling, decomposition product 1
Add 200 g of dextrin to 000 g, dissolve and homogenize with TK homomixer (manufactured by Tokushu Kika Kogyo Co., Ltd.), and spray-dry by a conventional method to prepare a lipase hydrolyzate of powdered whole milk powder (Reference product 1) did.

Reference Example 2 (Preparation of Protease Decomposition of Milk or Milk Processed Product) 200 g of skim milk powder was dissolved in 750 g of water with stirring, and the mixture was heated to about 75 ° C.
After cooling at 40 ° C., 0.1 g of coclase (trade name of protease manufactured by Sankyo Pharmaceutical Co., Ltd.) was added to 50 ml of water.
g, and the mixture was enzymatically decomposed at 40 ° C. to 45 ° C. for 24 hours. After completion of the enzymatic decomposition, the enzyme was deactivated by heating at about 85 ° C. for 15 minutes. After cooling, 1000 g of this decomposition product
Was mixed with TK homomixer (manufactured by Tokushu Kika Kogyo Co., Ltd.), homogenized, and spray-dried by a conventional method to prepare a protease-decomposed product of powdered skim milk powder (Reference product 2).

Example 1 1 part by weight of Reference 1 and 0.5 part by weight of Reference 2 were mixed to prepare a feed additive of the present invention (Product 1 of the present invention).

Example 2 (Preparation of lipase and protease hydrolyzate of milk or processed milk product) 200 g of whole milk powder was dissolved in 750 g of water with stirring, and the mixture was heated to about 75 ° C.
After cooling at 40 ° C. for 20 minutes, 0.4 g of lipase (trade name of lipase manufactured by Tanabe Seiyaku Co., Ltd.) and 0.2 g of protease P3G (trade name of protease manufactured by Amano Pharmaceutical Co., Ltd.) are dissolved in 50 g of water. Add 40
Enzymatic degradation was carried out at a temperature between 45 ° C and 45 ° C for 24 hours. At this time, the decomposition rate of the lipid (acid value / saponification value × 100) was 60%.
After completion of the enzymatic decomposition, the enzyme was deactivated by heating at about 85 ° C. for 15 minutes. After cooling, dextrin 2 was added to 1000 g of the decomposition product.
Add 00g, dissolve and homogenize with TK homomixer (manufactured by Tokushu Kika Kogyo Co., Ltd.), and spray-dry by a conventional method to prepare lipase and protease hydrolyzate of powdered whole milk powder (Product 2 of the present invention) did.

Example 3 The feed additives obtained in Reference Example 1, Reference Example 2, Example 1 and Example 2 were added to a formula feed for rearing piglets and a field test was carried out in the following manner. (1) Combined feed for test: Reference 1, Reference 2, Inventive 1 and Inventive 2
Was used as a test group, and a non-added feed was used as a control group.

[0027]

Table 1: Formulated feed for suckling pigs

(2) Test pigs: Weaned piglets (three-way hybrid) having an average age of 25 days were used in units of three pigs per experiment. (3) Test method: It was a palatability test by the cafeteria method, and the period was 5 to 7 days. (4) Test results: The results are shown in Table 2. As is evident from the results in Table 2, the feeds of the test plots containing the feed additive of the present invention significantly increased the intake and exhibited a remarkable palatability improvement effect as compared with the control. In addition, the feed additive using the lipase-decomposed product and the protease-decomposed product of the present invention in combination was superior in the palatability-improving effect as compared with the feed containing the lipase-decomposed product or the protease-decomposed product alone.

[0029]

[Table 2] Table 2: Test results

Example 4 The feed additives obtained in Reference Example 1, Reference Example 2, Example 1 and Example 2 were added to dog food for dogs (wet type), and a test was carried out in the following manner. (1) Dog food: A dog food prepared by adding 0.1% by weight of each of reference products 1, reference product 2, present invention product 1 and present invention product 2 to a commercial product (petegry cham, master food) was used as a test group, and no dog food was added. The feed was used as a control. (2) Test dog: Adult dogs (small to medium sized dogs of 2 to 5 years old, regardless of breed) were used in units of 5 per experiment. (3) Test method: A palatability test by the cafeteria method was performed for a period of one day. (4) Test results: Table 3 shows the results. As is clear from the results in Table 3, the dog foods in the test groups to which the product of the present invention was added significantly increased the intake,
It showed a remarkable palatability improvement effect as compared with the control.

[0031]

[Table 3] Table 3: Test results

[0032]

According to the present invention, a lipase-decomposed product and a protease-decomposed product of milk or a processed milk product are incorporated into livestock such as cattle and pigs, poultry such as chickens, and feed for pet foods such as dogs and cats. Thus, a feed additive capable of enhancing palatability and increasing the amount of intake to promote growth can be provided.

 ────────────────────────────────────────────────── ─── Continuing on the front page (72) Inventor Takayuki Inai 335 Kariyuku Hasegawa, Kagawa, Nakahara-ku, Kawasaki-shi, Kanagawa F-term in the Flavor Research Laboratories F-term (reference) 2B005 AA05 AA05 BA01 BA04 BA07 DA01 EA01 EA12 2B150 AA01 AA02 AA03 AA04 AA05 AA06 AB02 AB04 BB04 BB07 CC11 CC12 DF10 DF15

Claims (1)

[Claims] 1. A feed additive for livestock, poultry, and pet food, comprising as an active ingredient an enzymatically decomposed product of lipase and protease of milk or processed milk products.