JP2001120581A - Instrument and method for transplant of animal embryo - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a transplant instrument which minimizes disposable parts, simplifies the operation, reduces running cost, and can be safely, easily and rapidly set even in a complicated working site. SOLUTION: This transplant instrument comprises a flexible inner pipe 2 integrated with a pouring tip 3 at a tip thereof, a hard outer pipe 1 fitted to the inner pipe 2 except the pouring tip 3, and a push-out rod 8 having a flexible tip part 9 to push out a liquid embryo 6 in a straw pipe 5 fitted to the inner pipe 2 by inserting the tip part 9, and a straw pipe pull-out rod 15 having a threaded bar 14 to be screwed in the straw pipe 5 at the tip combined therewith.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a device and a method for transferring an animal embryo (fertilized egg).

[0002]

2. Description of the Related Art Embryo transfer technology is most widely used for cattle, and will be described using cattle as an example. However, this technique uses hormonal agents to transfer a large number of follicles to the ovaries of embryo-bearing cows at once. This is a technique for obtaining a large number of genetically excellent fertilized eggs, transplanting them into embryo-bearing cows and giving them pregnancy to obtain litters. The aim is to improve the efficiency of fetus improvement and reproduction.

[0003] As a device for transplanting the embryo to a recipient cow, there is a device disclosed in Japanese Patent Publication No. 61-36935, but this invention provides a small hole in the tip side of the inner pipe of a double pipe. After passing the cervix smoothly through a rigid double pipe, a flexible tube extends from its stoma along the curvature in the uterine horn, causing damage to the endometrium and bleeding, or penetrating the uterine wall Embryos can be implanted deep into the uterus without accidents.

[0004] However, in the present invention, it is necessary to fill the flexible tube with the germ fluid in advance, but it is extremely difficult to perform such work smoothly and cleanly at the work site, and it is difficult to spill expensive embryos. It may lead to accidents such as contamination of the mother's uterus.

[0005] For this reason, in recent years, a telescopic-type instrument for transplantation using a straw tube containing an embryo has been used. As shown in FIG. 10, the instrument has a hard sheath 101, a stainless steel outer tube 102 mounted in the sheath 101, and a tip poured into the outer tube 102 until the instrument passes through the cervix. Ten
3 and a stainless steel inner tube 105 connected to the flexible tube 104.
Inside, the straw tube 106 pushed into the pouring nozzle 103 side
And a plunger 107 that can be inserted into the straw tube 106
And a push rod 108 made of stainless steel for pushing the plunger 107 is inserted.In this state, the cervical canal is passed, and when the tip reaches an appropriate position in the uterine horn, the inner tube 105 is pushed in. Pouring tip 103 at the tip from pod 101
Together with the flexible tube 104 as shown in FIG.

[0006] Since each protruding portion is made of a flexible material, it is curved along the bend of the uterine horn and does not break through or damage the uterine horn. And flexible tube 104
And plunger 107 and pod 101 are disposable types,
There is an advantage that germs are less likely to enter the uterus.

[0007] However, since the three parts are disposable, not only the running cost is high but also the flexible tube may fall off from the main body when operated violently. Furthermore, the site of this kind of work is often complicated and dirty, and there is a disadvantage that it is only possible for an expert to remove the disposable part and replace it with a new one in such a site.

[0008]

SUMMARY OF THE INVENTION The present invention has been made in view of the above circumstances, and provides a seed transplanting device which can reduce the running cost by minimizing disposable parts and can be set safely, simply and quickly even in a complicated work site. The purpose is to do so.

[0009]

SUMMARY OF THE INVENTION The gist of the present invention is to provide a flexible inner tube integrally formed with a pouring tip at the tip and to be fitted to this inner tube except for the pouring tip portion. An animal embryo transfer device comprising a rigid outer tube and an extruding rod which has a flexible distal end portion and can push out embryo fluid in a straw tube attached to the inner tube by inserting the distal end portion. In addition, a straw tube drawing rod provided with a thread that can be screwed into the straw tube at the tip is combined with this.

[0010] The inner tube is desirably entirely flexible, and at least the tip is flexible. The pouring tip formed at the tip has a pouring hole on the trunk side. The dispensing tip is formed integrally with the inner tube and cannot be removed from the inner tube. It is desirable that both materials be made of plastic and integrally formed. On the other hand, the outer tube is a hard tube made of stainless steel, and is used by being fitted around the inner tube except for the pouring tip portion.

[0011] A straw tube containing a germ fluid for transplantation is arranged in the inner tube. Its end is almost in contact with the pouring tip on the inside, and on the opposite side, the tip has flexibility, and by inserting this tip, the germ fluid in the straw tube attached to the inner tube is removed. There is an extruder rod that can be extruded. Such a transplanting device is combined with a straw tube drawing rod provided with a thread that can be screwed into the straw tube at the tip.

According to the method of the present invention, a straw tube containing germ fluid is inserted into a flexible inner tube having an ejection tip integrally formed at the tip, and then the inner tube is removed except for the ejection tip. A rigid outer tube is fitted over the whole, and an extruding rod having a flexible tip is set on the inner tube. In this state, the whole is inserted into the vagina. Extrude the flexible inner tube at an appropriate position in the uterus to push the ejection tip deep into the horn of the uterus, and then push the push rod in to release the germ fluid in the straw tube from the ejection tip to the back of the uterus. Then, after removing the whole from the vagina, discard the used inner tube together with the straw tube, then disinfect the outer tube, fit the outer tube into the inner tube containing the new straw tube, and push the rod into this. Is set, and transplantation is performed in the same manner as described below.

[0013] Another method of the present invention is to insert a straw tube containing germ fluid into a flexible inner tube having an ejection tip integrally formed at the tip, and then remove the ejection tip and remove the inside of the straw tube. A hard outer tube is fitted over almost the entire tube, and a push rod having a flexible tip is set on the inner tube. In this state, the whole is inserted into the vagina, and the tip of the tip is discharged. Extrude the flexible inner tube beyond the cervix at an appropriate position in the uterus to reach the deep part of the uterine horn, and further push the push rod to push the germ fluid in the straw tube out of the tip and into the inner part of the uterus. After removing the whole body from the vagina, remove the push rod from the inner tube, and instead insert a straw tube drawer with a thread that can be screwed into the straw tube at the tip, and partially insert it into the straw tube. Screw in the straw tube, take out the outer tube,
This is a method of disinfecting the inner tube and the extruding rod, attaching a new straw tube to the inner tube, setting as described above, and transferring the embryo to another conception animal.

[0014]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Reference numeral 1 in FIG. 1 denotes an outer tube made of stainless steel, and 2 in FIG. Reference numeral 4 denotes a pouring hole formed on the body side of the pouring tip 3. Of course, this hole 4
Is in communication with In the pouring tip 3, there are two steps, the diameter of the central pouring hole 4 is almost equal to the inner diameter of the outer tube 1, and the tip from that portion is almost equal to the diameter of the outer tube 1. The inner diameter of the inner pipe 2 is equal to the inner diameter of the inner pipe 2 of the pouring hole 4. Therefore, at the time of entry into the uterus, the outer tube 1 is fitted to the portion of the pouring hole 4 to close the pouring hole 4, so that the body fluid in the uterus does not enter the pouring hole 4.

Reference numeral 5 in FIG. 3 denotes a straw tube containing a germ fluid 6 and a cotton plug 7 packed at one end. The straw tube 5 is inserted into the inner tube 2 on the side of the pouring tip 3. . 4 in FIG.
It is a push rod, and its tip is a flexible portion 9 in a state where a piano wire is twisted. As shown in FIG. 5, the flexible portion 9 is inserted from the vaginal opening 10 with the tip partly inserted into the straw tube 5 and passes through the cervix 11.

After passing through the cervix 11, the inner tube 2 is pushed out at an appropriate position. By this operation, the distal end of the inner tube 2 and the pouring tip 3 protrude from the outer tube 1 and are positioned in the uterine horn 12 in a flexible state in an appropriately curved state. In particular, the pouring tip 3 reaches the back of the uterus 13. FIG. 6 shows this state.

In this state, when the push rod 8 is pushed in as shown in FIG. 7, the embryo fluid 6 in the straw tube 5 is discharged from the pouring hole 4 of the pouring tip. After the ejection, the whole is pulled out of the vagina, and then the push rod 8, the inner tube 2, and the outer tube 1 are disassembled. In the next use, the push rod 8 and the outer tube 1 are disinfected, and the straw tube 5 is used.
And the inner tube 2 is replaced with a new one. Similarly assembled, the body is inserted into the vagina.

When the inner tube 2 is not made disposable,
A straw pipe pull-out rod 15 having a thread 14 formed at the tip is inserted into the inner pipe 2, the thread 14 is screwed into the straw pipe 5 (see FIG. 9), and the straw pipe 5 is pulled out as it is.
The whole is then dismantled and disinfected, set again and the transplantation to another recipient animal resumed.

[0019]

As described above, the present invention comprises only an outer tube, an inner tube integrated with a pouring tip, and an extruding rod. Since the number of parts is small, the manufacturing cost can be reduced, and the inner tube is disposable. If, after embryo transfer, the main body is pulled out of the uterus, the inner tube is discarded, only the outer tube and the push rod are disinfected,
Then insert the straw tube and push rod into the new inner tube,
Since the setting is completed by fitting the outer tube to the outer tube, the setting operation in several uses can be performed very simply and quickly.

[0020] Further, since the dispensing tip is integrated with the inner tube, there is no risk of parts falling off in the uterus, and there is no part that can fall off, so that it is extremely safe.

On the other hand, in the case where a straw tube extraction rod provided with a thread at the tip is combined, the inner tube can be reused after use. That is, after the transplantation operation, the straw tube removal rod is screwed into the straw tube remaining in the inner tube, the straw tube is pulled out, the inner tube is emptied, and the entire tube is disinfected together with the outer tube and the push rod, The inner tube can be reused, and the disposable and reusable inner tube can be selected according to the user's preference.

[0022] The method of transplanting an embryo using such a device is also simple and quick, and is less likely to cause errors.

[Brief description of the drawings]

FIG. 1 is a sectional view of an outer tube.

FIG. 2 is a partial cross-sectional view of the inner pipe and a partially enlarged view thereof.

FIG. 3 is a sectional view of a straw tube.

FIG. 4 is a side view of a push rod.

FIG. 5 is a partial cross-sectional view and a partially enlarged view of the transplantation device in a set state.

FIG. 6 is a simplified view of the implanting device inserted into the uterus and the inner tube is projected.

FIG. 7 is a partial cross-sectional view of the transplantation device inserted into the uterus and the inner tube is projected.

FIG. 8 is a side view of a straw pipe drawer bar.

FIG. 9 is an enlarged cross-sectional view of a state in which the tip of a straw pipe drawer rod is screwed into the straw pipe.

FIG. 10 is a sectional view of a telescopic device in a set state.

11A and 11B are cross-sectional views of a telescopic device with a pouring tip protruding, and FIGS.

[Explanation of symbols]

1-stainless outer tube, 2-flexible inner tube, 3-pour tip, 4-pour hole, 5-straw tube, 6-germ fluid, 7-cotton plug, 8-push rod, 9-possible Flexure, 10-vaginal, 11-cervix, 12-uterus, 13-back of uterus, 14-thread, 1
5-straw tube drawer,

Claims (4)

[Claims] 1. A flexible inner tube integrally formed with a discharge tip at a tip, a hard outer tube fitted on the inner tube, and a tip having flexibility, and insertion of the tip. An animal embryo transfer device comprising an extruding rod capable of extruding embryo fluid in a straw tube attached to the inner tube 2. The animal embryo transfer device according to claim 1, wherein a straw tube pulling rod provided with a thread that can be screwed into the straw tube at the tip is combined. 3. A straw tube containing germ fluid is inserted into a flexible inner tube having a pouring tip integrally formed at the tip,
Next, a hard outer tube is fitted over substantially the entire inner tube, and an extruding rod having a flexible end is set on the inner tube. In this state, the whole is inserted into the vagina, and the tip of the inner tube is inserted. The ejection tip crosses the cervical canal, pushes the flexible inner tube at an appropriate position in the uterus to make the ejection tip reach the deep part of the uterine horn, and further pushes the pushing rod out to remove the germ fluid in the straw tube from the ejection tip. After discharging into the back of the uterus and then removing the whole from the vagina, the inner tube is discarded together with the straw tube, the outer tube and the push rod are disinfected, and then a new straw tube and inner tube are set as described above, Method for transplanting a bovine embryo, wherein the embryo is transferred to another cow 4. A straw tube containing germ fluid is inserted into a flexible inner tube having a pouring tip integrally formed at the tip,
Next, a hard outer tube is fitted over substantially the entire inner tube, and an extruding rod having a flexible end is set on the inner tube. In this state, the whole is inserted into the vagina, and the tip of the inner tube is inserted. The ejection tip crosses the cervical canal, pushes the flexible inner tube at an appropriate position in the uterus to make the ejection tip reach the deep part of the uterine horn, and further pushes the pushing rod out to remove the germ fluid in the straw tube from the ejection tip. After releasing the whole uterus from the back of the uterus and then taking out the whole from the vagina, insert a straw tube with a threaded tip at the tip instead of the extrusion rod, pull out the tip with the straw tube with the tip screwed into the straw tube, A method for transplanting an embryo for cattle, comprising setting the inner tube after disinfection as described above, and performing embryo transfer to another cow.