KR970007677B1 - Artificial insemination and embryo transfer device - Google Patents

Abstract

The device for injecting germinal cell to mammalian genital is simply operated, is easily inserted to womb tubes, and prevents womb tubes from being damage upon injection. The injection device comprising cytula within straw(38) is inserted to vagina(8) so that thin film cap(68) fixed at front end of auxiliary cylinder(66) in the second contamination protecting mean(44) is located to inlet of womb tubes(2). In this state, unilateral flange(124) of grasping means(114) is contacted with the flange(104) of support cylinder(102), and support flap(116) is located in proximity to flange(64) of auxiliary cylinder(66). Subsequently, the tube(106) of the first contamination protecting mean(42) enclosing tip(34) is directly contacted with womb tubes(2) to fetch. Thus, the tube(106) of first contamination protecting mean(42) is introduced to womb tubes(2) with enclosing streamline front end(48) of chip(34).

Description

Device for injecting germ cells into mammalian genitals

1 is a schematic representation of the human genitalia.

2 is a schematic representation of the genitals of cattle.

3 to 5 are cross-sectional views showing devices for injecting germ cells into the genitals of conventional stray animals.

Figure 6 is a cross-sectional view showing a first embodiment of a device for injecting germ cells into the genitals of stray animals according to the present invention.

7 and 8 are cross-sectional views showing modifications of the coupling and fastening structure of the tip shown in FIG.

9 is a cross-sectional view showing a modification of the second pollution prevention means of the injection device shown in FIG.

FIG. 10 is a right side view of the injection device shown in FIG.

FIG. 11 is a sectional view showing a modification of the locking means of the piston shown in FIG.

12 is a cross-sectional view showing a second embodiment of a device for injecting germ cells into the genitals of a mammal according to the present invention.

FIG. 13 is a sectional view showing the cross-sectional shape of the body of the tip shown in FIG.

14 is a cross-sectional view showing a modification of the cross-sectional shape of the body of the tip shown in FIG.

FIG. 15 is an excerpt sectional view showing a modification of the gripping means shown in FIG.

FIG. 16 is a sectional view showing a modification of the injection apparatus shown in FIG.

FIG. 17 is an excerpt sectional view showing a modification of the support cylinder shown in FIG.

18 to 20 are cross-sectional views showing an artificial insemination process by the apparatus for injecting germ cells into the genitals of stray animals according to the first embodiment of the present invention.

21 to 25 are cross-sectional views showing a fertilized egg transplant process by the apparatus for injecting germ cells into the genitals of stray animals according to a second embodiment of the present invention.

* Explanation of symbols for main parts of the drawings

30: cylinder 34: tip

38: straw 40: piston

42: first pollution prevention means 44: second pollution prevention means

52: drain port 58: cylindrical sheet

66: auxiliary cylinder 68: thin film cap

74: adapter 96: notch

98: locking means 102: support cylinder

106: tube 114: gripping means

The present invention relates to a device for injecting germ cells, such as semen or fertilized egg, into the genitals of stray animals for artificial insemination and fertilized egg transplantation.

In general, artificial insemination refers to the act of injecting semen into the genitals, and fertilized egg transplantation refers to the act of injecting fertilized eggs into the genitals.

FIG. 1 shows the genitals of a human and FIG. 2 shows the genitals of a cow.

Insemination requires the injection of semen into two-thirds of the cervix (2) or the inlet (4a) of the uterine body (4) shown in FIGS. 1 and 2, and the fertility rate is high. In humans without uterine angles and well-developed uterine bodies, fertilized eggs should be injected into the core of the uterine body (4) to have high conception rates. It is known that fertilization rate is high only when the fertilized egg is injected into the tip 6a which is the core of the angle 6.

In addition, conditions for improving fertility during artificial insemination and fertilized egg transplantation should be easily passed without damaging the cervix (2) when the device for injection of germ cells such as semen or fertilized egg passes through the cervix (2). Also, it should not be contaminated with bacteria or foreign objects.

Given these conditions, artificial insemination is performed non-surgically, and fertilized egg implantation is performed surgically or non-surgically depending on the type of animal, the size of the animal, or the degree of transplantation technique.

Most of the implantation of human fertilized egg is performed by non-surgical method through the cervix, and the human genitalia is anatomically shaped like a terminal arch, unlike other mammals as shown in FIG. Since the uterine body (4) is well developed, implantation of the fertilized egg in the middle of the uterine body (4) or better implanted.

The structures and tissues of the cervical canal of all stray animals, including humans, are generally similar, but there are many differences in size. For implantation and artificial insemination through the cervix, the injection device must pass through the cervix.

Because other mammary glands except humans have very small uterine bodies and well developed uterine angles, the fertilized egg transplantation of these mammals must inject fertilized eggs into the center of the bent uterine angle after passing through the cervical canal like humans. In this case, the fertilization rate is high when artificial semen is injected with semen into the deep part of the cervix or the inlet 4a of the uterine body in the same manner as a human fertilized egg transplant.

In addition, the technology of artificial insemination or fertilized egg implantation for humans and animals is the same technology and the principle of the injection device is the same. However, the size of the implant only varies depending on the size of the animal.

Therefore, hereinafter, mainly cows will be described by way of example.

The fertilized egg transplantation method in cows can be broadly classified into a surgical method, that is, a laparotomy method and a non-surgical method, that is, a recto-vaginal method.

Surgical method of the above method is to anesthetize a fertilized egg in order to inject the fertilized egg into the egg, then open surgery, and then inject the fertilized egg using a syringe into the tip (implantation site of the uterus). do.

The above method injects the fertilized egg into the fertilized egg at the tip of the uterus, which has the best environmental conditions and the best effect on the development, and can easily prevent contamination. It is said to be about 10% higher than the conventional method.

However, the above-described methods are avoided because they have problems such as expensive procedure cost, long time of procedure, stress of animals, and impossibility of repeated use of poached cows.

As a result, fertility implantation method through cervical canal is widely used due to low cost, short procedure time, and many advantages that can be used repeatedly because it does not give shocking stimulus to egg. .

In order to perform fertilized egg implantation and artificial insemination through the cervix, the injection device must pass through the vagina 8 and the cervix cannula 2 shown in FIG. 2 in particular. (6) The conception rate can be improved only by discharging at the tip end portion 6a, which is a core part.

The cervix (2), which isolates the vagina (8) and the cervix (6) during all periods except labor, prevents bacteria in the vagina (8) from entering the uterus. Many weights are closed by severe contractions. In addition, the vertebral wall, which forms the outer wall of the cervical canal 2, is contracted to form a tissue of cartilage (hard tissue similar to cartilage), so that there is some relaxation at the time of estrus, but it is completely closed at other periods. The procedure of fertilization is essential that the injection device must pass through the cervix (2), but the wall is severely constricted, it is difficult to pass unless you are a highly skilled technician. Accordingly, if excessive force is applied to the injection device while passing through the cervix (2), the mucosal epithelial cells of the cervix (2) are peeled off, causing a wound, and the bleeding blood acts as a toxin on the germ cells. Inflammation by the wound may cause a decrease in fertility.

Therefore, the tip of the injection device is used to pass through the extracted cervix 2, but it is difficult to pass the tip of the injection device even if a skilled person does not hurt the cervix 2. In this case, the injector may be thinned to easily pass the cervix, but in this case, irregular protrusions and irregular depressions in the cervix may occur, so that the tip of the injector is thin, which risks puncturing the cervix or uterine angle. Will be implied.

An apparatus used for insemination and fertilization of an egg in a non-surgical manner is the infusion apparatus disclosed in French Patent No. 7,621,438. However, this infusion device is not possible to inject the fertilized egg into the deep part of the uterus without stimulating the cervix (2) and the endometrium in a sterile state, so related experts seek to solve this defect.

3 is a cross-sectional view showing an embodiment of a conventional injection device. The injection device shown in FIG. 3 includes a straw 10 into which germ cells such as semen or fertilized egg are loaded, an intermediate cylinder 11 into which the straw 10 is inserted, and an insertion into the intermediate cylinder 11. A piston (12) forcibly discharging germ cells in the straw (10), a large cylinder (14) into which an auxiliary ring (13) with a passage (13a) is inserted and inserted into the outside of the intermediate cylinder (11); And a ring 14a for fixing the large cylinder 14.

Therefore, after withdrawing the piston 12 from the intermediate cylinder 11, inserting a straw 10 loaded with germ cells such as semen or fertilized egg into the front end of the intermediate cylinder 11, and then to the outside of the intermediate cylinder 11 The large cylinder 14 made of disposable plastic is engaged and the ring 14a is pulled and fixed.

The fertilized egg transplant is equipped with the straw 10 loaded with the fertilized egg 15 in the injection device, and through the quality of the oviparous cow (8) and the cervical canal (2), which are intended to operate the large cylinder (14), according to the skill of the operator. The fertilized egg (15) in the straw (10) in the straw (10) is pushed into the uterine angle (6) as much as possible with minimal stimulation to the endometrium and then advances the piston (12) that has been drawn out to the rear of the intermediate cylinder (11). The fertilized egg is injected into the position before and after the middle part 6b of the uterine angle 2 in the state drained with the medium 16.

The implanted egg is implanted in the middle part 6b of the uterine angle 6 instead of the tip 6a of the uterine angle 6, which maintains the best conditions for implantation. It is pointed out as the biggest cause.

On the other hand, during insemination, a semen loaded straw is inserted into the injection device, and then the intermediate cylinder 11 is sequentially passed through the vagina 8 and the cervical canal 2 as described above. The semen is drained by draining at the inlet 6c.

However, such an injection device has the following problems.

First, the assembly is inconvenient because the assembly of the large cylinder 14 between the intermediate cylinders 11 by pulling the ring 14a is inconvenient, and a case of separation occurs during the procedure.

Second, since numerous bacteria inhabit the entrance side of the vagina (8) and cervix (4) during artificial insemination or fertilization, the tip of the large cylinder (14) may pass through the vagina (8) and the cervix (2) in order. When the path 13a, which is the tip of the auxiliary ring 13, is filled with pathogenic or non-pathogenic bacteria and injected with semen or fertilized egg into the uterine angle (6), the invading bacteria are secreted from the uterine angle (6) to make a drainage area. The uterine oil (nutrition necessary for the fertilized egg to grow) rapidly proliferates and changes the environment in the uterus (pH change, inflammation) and, depending on the degree of change, stops the embryo development and kills or inhibits the conception rate. Degraded.

Third, during insemination and fertilization of the embryo implantation, the operator's one hand is placed into the rectum of the cow and senses the position at which the tip of the injection device passes, and the other hand pushes the injection device.

At this time, the inner wall of the cervical canal (2) is an irregular limbic ring, while the inner diameter of the cervical canal is bent, while the distal end of the injector is hard and straight, and the opening is large and resists by the projection of the limbic ring. (2) had a lot of difficulties to pass, accordingly, if you apply excessive force will damage the mucous membrane of the cervix can cause output and inflammation.

Fourth, in fertilized egg implantation, the fertilized egg is implanted well when the fertilized egg is drawn out as far as possible to push the distal end of the injection device that has passed through the cervical canal 2 as close to the distal end portion 6a as the core of the bent uterine liver 16. Since it is a straight line which cannot be bent, it is difficult to insert the injection device more than the middle portion 6b of the uterine angle 6.

However, the desire to inject fertilized eggs into the deep end of the uterine angle (6) (6a) due to the excessive force to push the injection device deeper, and accordingly severe pressure between the distal end of the injection device and the endometrium (6) The wound caused by friction causes bleeding, and the blood plays a toxin role in the fertilized egg, which prevents the fertilized egg from normal cell division.

Fifth, since the tip of the injection device is a rigid body and a straight line, the tip of the injection device was inserted into the abdominal cavity through the cervical canal or the uterine angle by a slight mistake due to excessive insertion.

Sixth, because the drainage port of the fertilized egg is opened in a straight line (開口) is blocked in pressure contact with the inner lining of the bent uterine angle (6) was not able to drain the fertilized egg smoothly.

Therefore, an injection device for reducing the above problem is presented in US Patent No. 4,701,164, which is different from the injection device in FIG. 3 as shown in FIG. The other tip 18 in which the drain port 18a was formed in both sides is adhesively fixed, and the position of the drain port 18a is changed. Since the head of the tip 18 is circular without drainage, this infusion device may pass through the cervix 2 slightly more easily than the infusion device shown in FIG. Since it is made of hard plastic or metal, which is not flexible at all, the cervix (2) is also damaged when the fertilized egg is injected.

And the two drainage holes (18a) formed on both sides of the neck of the tip (18) has improved the element blocking the opening in the bent uterine angle (6), but the drainage and drainage when a small amount of suspended fertilized egg injected into the medium Uterus mucus is introduced around the bulb and the fertilized egg injected there is a high risk of loss.

In addition, when the injection device passes through the vagina (8) and cervix (2), the mucus is filled in the opening of the drain port (18a), there is a concern that the fertilized egg is attached to the mucus, as well as bacterial contamination, there is a fear of missing Since 18 is fixed in a straight line and floating, the workability is poor, and since the injection device is straight, it is impossible to inject the tip 6a, which is the core of the bent uterine angle 6, so that the conception rate decreases. .

Accordingly, an injection device for preventing the occurrence of wounds of the cervix and the cervix due to the rigidity of the tip portion during insertion, which is a problem of the injection device, is disclosed in British Patent No. 2,070,437.

In the injection device, as shown in FIG. 5, a semi-rigid nozzle 20, which is an extremely small part, is fixed to the tip of the large cylinder 19, and the center of the nozzle 20 is fixed. Is different from the injection device shown in FIG. 4 in that a passage 20a for discharging the fertilized egg is formed.

However, since the nozzle 20 of the injection device is semi-solid, it is possible to slightly alleviate the occurrence of a wound on the contact surface when inserting the injection device into the cervix 2 and the uterine angle 6, rather than a solid material. As described above, since the tip portion is not flexible and flexible, it is difficult to pass through the cervix 2, and since the tip portion is straight, it is difficult to insert the tip portion 6a, which is the core portion of the uterine angle 6.

In addition, U.S. Patent No. 4,846,785 describes a mammalian artificial insemination, fertilized egg implantation and follicular fluid collection device, the core function of which is a laparoscopy technique through a transperitoneal, which does not undergo laparotomy. In order to insert the laparoscope and the injector into the abdominal cavity, two small holes are made in the abdominal wall, and the injection device equipped with metallic needles is inserted through the holes. In other words, this method can be called a surgical method.

In addition, the infusion device described in the U.S. patent that the cervix can be shortened and relatively loosely closed, and that the tissue constituting the wall of the cervix can be artificially inseparable only for deer or goats that are flexible and can be relaxed ( This injection device is used for the artificial insemination by forceps or the planar method of inserting the injection device after visually confirming the entrance of the cervical canal by remodeling the vagina), but has the following problems. When inserting the tip into the cervical canal, there is a constant gap between the tip of the tip that is subjected to resistance and the joint that supports and secures the tip. it's difficult. Furthermore, in order to prevent the tip from bending, when the tip is made shorter or made of a harder material, there is a closed end of the problem of the conventional injection device shown in FIG. In particular, such an injection device cannot be used for artificial insemination by the rectal method of large animals such as cows or horses in which the cervix is well-developed and the tissues constituting the wall are formed of cartilage-like pendulum. In addition, this infusion apparatus cannot prevent contamination of the tip vagina and cervix during artificial insemination.

Accordingly, an object of the present invention is to provide a device for injecting germ cells into the genitals of stray animals that can be easily manipulated, easily inserted into the cervix and prevented from damaging the cervix.

Another object of the present invention is to prevent the contamination of the tip and cylinder by bacteria and foreign substances in the vagina and cervix when inserted into the vagina and cervix can be inserted into the genitals in a sterile state that can improve the conception rate To provide a device for injecting germ cells into the genitals of.

Another object of the present invention is to provide a device for injecting germ cells into the genitals of a mammal capable of injecting fertilized eggs into the tip of the uterine angle, which is an implantation site in the natural state, without damaging the cervix and uterine angle. have.

The object of the present invention is a cylinder having a flange formed on one side, the support jaw is formed on the inner surface, a straw inserted into the cylinder is supported on the support jaw of the cylinder, the germ cells are prolonged, and the straw A flexible tip having a distal end of the cylinder, a distal end communicating with the straw, and movably inserted into the cylinder, and guided along the support jaw of the cylinder to the straw; A piston for forcibly discharging the loaded germ cells through the drain hole of the tip, a locking means installed between the cylinder and the piston to prevent the piston from flowing, and inserted into the outer side of the cylinder to deform the tip. And a support cylinder having a flange formed on one side, and installed outside the support cylinder and the tip. First pollution prevention means for preventing their contamination by the cervix during passage of the cervix, and is installed on the lateral side of the first pollution prevention means with the tip wrapped, preventing the first pollution by the vagina when the vaginal passage A device is provided for injecting germ cells into the genitals of a mammal comprising means and a second contamination prevention means for preventing contamination of said tip.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

First, the configuration of the first embodiment of the apparatus for injecting germ cells into the genitals of a stray animal according to the present invention will be described with reference to FIGS. 6 to 11.

The injection device of the first embodiment, as shown in Figure 6, the cylinder 30 is formed with a flange 32 on one side, and the tip 34 of the flexible material is coupled to the other side of the cylinder 30 And a straw 38 inserted into the cylinder 30, supported by the support jaw 36 of the cylinder 30, fixed to the tip 34, and suitable for storing germ cells such as semen or fertilized egg. A piston 40 movably installed on the inner surface of the 30 and inserted into the outside of the cylinder 30 so as to prevent contamination of the cylinder 30 and a piston 40 for forcibly discharging the germ cells loaded on the straw 38. The first pollution prevention means 42 and the second pollution prevention means 44 which surround the outside of the tip 34 and are inserted outside the first pollution prevention means 42 to prevent contamination of the tip 34 and the tip 34. It is configured to include. The support jaw 36 of the cylinder 30 prevents the straw 38 from being inserted into the cylinder 30 more than necessary and prevents the movement of the piston 40 when draining the semen or fertilized eggs loaded in the straw 38. It serves to guide precisely towards the straw 38.

The cylinder 30 and the piston 40 are made of a rather rigid material, but the tip 34 is a flexible material, for example, so as not to damage the cervix and the cervix when passing through the cervix and the cervix at the time of the procedure. For example, it is preferable to manufacture the material of any one of non-toxic silicone rubber, poly, vinyl, natural and synthetic rubber. In particular, the tip 34 is a flexible material that can be advanced to the tip of the uterine angle without damaging the uterine angle while deformed and bent according to the shape of the uterine angle when passing through the cervix and uterine angle in the case of fertilized egg transplant It must be constructed. The tip 34 is in contact with the body 46, a streamlined tip 48 suitable for easy insertion into the cervix and uterine angle during implantation and fertilization of the fertilized egg and the first contamination prevention means 42, It consists of the inclined middle part 50 to support, and the drain hole 52 suitable for the passage of the semen, the fertilized egg, and the medium in the straw 38. The inner diameter of the drain port 52 of the tip 34 may be appropriately set according to the purpose or method of use of the injection device. In particular, the diameter of the drain hole 52 of the tip 34 is formed to be much smaller than the diameter of the straw 38 so that the semen or fertilized egg remaining in the drain hole 52 when the medium and fertilized egg or semen in the straw 38 are discharged. And preferably the amount of medium is first. In addition, in the case of artificial insemination which may not use the second pollution prevention means 44, when the streamlined tip portion 48 of the tip 34 passes through the entrance of the cervix, the bacterial contamination or the mucous of the cervix can cause the tip ( It is preferable to make the diameter of the discharge portion 52a of the drainage port 52 smaller than the diameter of the inlet portion 52b of the drainage port 52 so as to minimize the entry into the drainage port 52 of 34. As described above, since the tip 34 is made of a flexible material, when the streamlined tip portion 48 of the tip 34 passes through the cervix, it bends freely along the labyrinth of the cervical canal wall. Therefore, while minimizing the irritation to the cervix can easily pass through the cervix. Accordingly, there is no need to use a cervical canal expander in artificial fertilization or fertilized egg transplantation in the heifer cow. On the other hand, when the length of the tip 34 is long, the injection device is mainly used for implantation of the fertilized egg (see FIGS. 12 and 24), and when the length is short, it is used for artificial insemination, but must be used separately. There is no. The reason is that even if the length of the cervix and uterine angle are different according to the type or size of the animal, the tip 34 moves along the cervix and uterine angle by the advance length of the cylinder 30, so that the injection device of the present invention Can be commonly used for insemination and fertilization. In particular, in the case of artificial insemination, the streamlined tip portion 48 of the tip 34 is inserted to 2/3 of the cervix or the entrance of the uterine body, and then drains the semen. Since it moves to the fallopian tube bulge which is a site | part, it does not matter even if the length of the tip 34 is short.

The tip 34 is formed by the boss portion 54 and the threaded portion 56 formed at the rear end thereof so that the tip portion 38 is not separated from the cylinder 30 and the straw 38 in the cervix and uterine angle during insemination or fertilization. The inner side of the open side) is coupled in an interference fit manner and simultaneously screwed to the distal inner side of the cylinder 30. In particular, when the tip 34 is separated from the cylinder 30 in the uterine angle at the time of implantation of the fertilized egg, it cannot be recovered without open surgery, and thus, the tip 34 must be firmly coupled to the cylinder 30.

The first pollution prevention means 42 is a cylindrical sheet 58 inserted into the outside of the cylinder 30 and supported by the inclined intermediate portion 50 of the tip 34, and the stepped portion 33 of the cylinder 30. Spring 60 for pressing the cylindrical sheet 58 toward the tip 34 so that the tip of the cylindrical sheet 58 elastically contacts the inclined middle portion 50 of the tip 34; It consists of a cylindrical holder 62 fixed to an outer surface of the cylinder 30 so as to surround the spring 60. The cylindrical seat 58 is inclined to the intermediate portion 50 of the tip 34 by the spring 60. The close contact prevents bacteria and mucus from the genitals from entering the outer surface of the cylinder 30 during artificial insemination or fertilization of the fertilized egg, thus preventing contamination of the cylinder 30. Further, the cylindrical sheet 58 ) Also supports the flexible tip 34. On the other hand, the grip ring 70 is movably inserted into the outer surface of the cylindrical holder 62. One end of the cylindrical holder 62 is provided with an annular protrusion 63 for preventing the gripping ring 70 from being separated from the cylindrical holder 62.

In addition, as shown in FIG. 6, an o-ring 43 is provided in the recess 41 of the piston 40 and is in contact with the inner surface of the cylinder 30. 40 is not separated from the cylinder 30.

The second pollution prevention means 44 includes an auxiliary cylinder 66 inserted into the outside of the tip 34 and the first pollution prevention means 42 and having a rectangular flange 64 (see FIG. 10) formed at one side thereof. It is composed of a thin film cap 68, which is fusion-fixed to the tip of the auxiliary cylinder 66, and is easily ruptured by the advance of the tip 34. The flange 64 of the auxiliary cylinder 66 serves as a handle capable of holding the auxiliary cylinder 66 by hand during implantation or artificial insemination. In particular, when an operator using a small hand uses the injection device, the thin film is moved forward by moving the cylinder 30 by holding the flange ring 64 of the holding ring 70 and the auxiliary cylinder 660 inserted into the cylindrical holder 62 with one hand. The cap 68 will be ruptured The structure of the thin film cap 68 and the fixing structure of the auxiliary cylinder 6 will be described in detail later.

FIG. 7 shows a modification of the fixing structure of the cylinder 30 and the tip 34 shown in FIG.

The embodiment shown in FIG. 7 has a structure in which the tip 34 is coupled and fixed to the straw 38 and the cylinder 30 by the adapter 74, and the stepped groove 76 is formed at the candidate of the tip 34. As shown in FIG. By inserting the capillary 77 and the step portion 78 of the adapter 74 in the stepped groove 76 of the tip 34 is fixed to each other inseparable in the same manner as the adhesive or ultrasonic melting point . The capillary 77 of the adapter 74 not only assists in bending the tip 34 during insemination and fertilization, but also serves to ensure that the tip 34 is firmly coupled to the adapter 74. In addition, the inner surface of the straw 38 is fixed to the outer surface of the coupling boss 80 of the adapter 74 by an interference fit method. The cylinder 30 is screwed with the screw part 82 formed in the outer surface of the adapter 74 through the screw part 72 thereof. On the other hand, the adapter 74 is formed with a through hole 84 that communicates with the drain port 52 of the tip 34.

8 shows a modification of the first pollution prevention means 42 shown in FIG. In this embodiment, the cylinder 30 and the tip 34 are connected via the adapter 74. When explaining the connection structure of the cylinder 30 and the tip 34 in detail, the capillary tube 77 and the step portion 78 of the adapter 74 is fixed to the stepped groove 76 of the tip 34, the cylinder The tip end of the 30 is screwed to the adapter 74 at the time of preparation, the straw 38 is fixed to the support jaw 36 of the cylinder 30 and the coupling boss of the adapter 74 by screwing the cylinder ( 80) is fixed by interference fit. The front end of the cylindrical sheet 58 of the first pollution prevention means 42 is integrally fused and fixed to the outer surface of the adapter 74 and the outer surface of the body 46 of the tip 34 by an appropriate technique. Therefore, when assembling the first pollution prevention means 42 to the cylinder 30 of the injection device, the straw 38 is inserted into the cylindrical sheet 58 integrally fused with the adapter 74 and the tip 34. After inserting to the outer surface of the cylinder 30, the rear end of the cylindrical seat 58 is grasped and rotated to assemble by screwing the threaded portion 82 of the adapter 74 to the threaded portion 72 of the cylinder 30. In the process, since the opening of the straw 38 is fixed to the coupling boss 80 by interference fit, the assembly is completed. Therefore, since the first pollution prevention means 42 is assembled to the cylinder 30 by the rotation of the cylindrical sheet 58, the assembly and the assembly of the tip and the tip 34 of the cylindrical sheet 58 are not contaminated. It's easy.

9 shows a modification of the second pollution prevention means shown in FIG. As shown in FIG. 9, the stretchable cap-shaped thin film tube 86 is fixed to the tip of the auxiliary cylinder 66 so as to be positioned inside the thin film cap 68. An annular protruding ring 88 is formed at one end of the stretchable cap-shaped thin film tube 86, and the annular protruding ring 88 is fitted to an outer surface of the stepped portion 90 of the auxiliary cylinder 66 to fix the ring ( 92). In addition, the other central portion of the stretchable cap-shaped thin film tube 86 is provided with a tearable portion 94 that can be ruptured by the forward movement of the streamlined tip portion 48 of the tip 34 during the procedure. If the rupturable portion of the cap-shaped thin film tube 86 is ruptured and formed in the hole, the ruptured hole is expanded by the extension of the cap-shaped thin film tube 86 in accordance with the forward movement of the streamlined tip portion 48 of the tip 34. The streamlined tip 48 of the tip 34 is exposed. On the other hand, the thin film cap 68 is fusion-fixed to the outer surface of the auxiliary cylinder 66 to surround the stretchable cap-type thin film tube 86. Therefore, when the tip 34 is advanced by the forward movement of the cylinder 30 in the state in which the injection device is inserted to the entrance of the cervical canal while the contamination is prevented from the vagina during implantation or fertilization of the fertilized egg, the thin film cap 68 ) Is ruptured and the tip 34 enters the cervical canal in a state surrounded by the cap-shaped thin film tube 86, and if the tip is further advanced, the tip 34 passes through the cervical canal and enters the widened uterine body. Subsequently, further movement of the tip 34 causes the streamlined tip 48 of the tip 34 to rupture the rupturable portion 94 of the capped thin film tube 86 and pass through the ruptured hole. In this case, since the thin film tube 86 is wrapped in the cervix, the outer surface of the tip 34 and the outer surface of the tip of the cylindrical sheet 58, the tip 34 and the cylindrical sheet 58 and the support cylinder is contaminated from the cervix It can be prevented at the source. The site where the tip 34 is exposed in the thin film tube 86 is exposed at the entrance of the uterine body or the uterine angle 6c during implantation of the midpoint fertilized egg of the cervix during insemination. Such adjustment may be performed by varying the stretch width of the thin film tube 86 and the elasticity of the material.

As shown in FIG. 10, the thin film cap 68 has a plurality of radial cut lines 96 so that the thin film cap 68 can be easily ruptured by the forward movement of the tip 34 at the time of artificial insemination or fertilization. ) Is formed. In particular, the thin film cap 68 is made of a material having flexibility and elasticity so that the thin film cap 68 is not broken when the thin film cap 68 is ruptured. The reason for this is that if debris is generated at the time of rupture of the thin film cap 68, it may be attached to the distal end portion 48 of the tip 34 by static electricity or mucus and enter the uterine shell, which may cause a great problem in breeding.

As shown in FIG. 11, a locking means 98 is provided between the cylinder 30 and the piston 40 to prevent the piston 40 from being separated from the cylinder 30 unless an external force is applied. The reason for installing the locking means 98 is to prevent the components of the injection device from being separated from each other when the injection device is inclined to one side during the procedure.

The locking means 98 has a structure in which the cylinder 30 is inserted and fixed to the flange 32. That is, a part of the cylinder 30 fixed to the flange 32 forms a ring-shaped leaf spring 100 by pressing, so that the ring-shaped leaf spring 100 pressurizes the outer surface of the piston 40. have.

Using the apparatus for injecting germ cells into the genitals of a mammal according to the first embodiment of the present invention configured as described above, the artificial insemination process will be described with reference to FIGS. 18 to 20 as follows. .

First, as shown in FIG. 18, the injection device in which the semen or fertilized egg is loaded into the straw 38 is inserted into the vagina, and the thin film cap fixed to the tip of the auxiliary cylinder 66 of the second pollution prevention means 44. The injection device is inserted into the vagina so that 68 contacts the protrusion at the entrance of the cervix 2. At this time, since the auxiliary cylinder 66 and the thin film cap 68 of the second pollution prevention means 44 surround the cylinder 30 and the tip 34, the cylinder 30 and Contamination of the tip 34 is prevented.

Thereafter, with the thin film cap 68 in contact with the entrance of the cervical canal 2, the cylinder 30 is advanced by holding the rectangular flange 64 of the auxiliary cylinder 66, as shown in FIG. Then, the tip 3 installed at the tip of the cylinder 30 is advanced, and the thin film cap 68 is widened in the shape of the streamlined tip of the tip 34 and inserted into the inlet 2a of the cervix 2. In contact with the cervix. In this state, since the thin film cap 68 is not ruptured, the cylinder 30 and the tip 34 are caused by bacteria or foreign substances at the inlet 2a of the cervix 2 which the vaginal mucosa extends and is in contact with the depression. ) Is prevented from being contaminated. Subsequently, further advance of the cylinder 30 causes the membrane cap 68 to rupture, and the tip 34 is inserted into the cervix 2 with minimal contact at the inlet 2a of the cervix 2. At this time, since the radial cut line 96 (see FIG. 10) is formed in the thin film cap 68 and has a suitable elasticity, the thin film cap 68 is not broken when the thin film cap 68 is ruptured. As a result, the tip 34 enters into the sterile cervix 2 in a state where bacteria or contaminants are not attached, thereby preventing contamination in the uterus.

In addition, as described above, since the tip 34 is formed of a flexible flexible body and the tip portion thereof is formed in a streamlined shape, the tip 34 is bent along the inside of the irregular posterior wall of the cervical canal 2 to facilitate the cervical canal. Can pass. As a result, no damage is done to the cervix 2. For this reason, the use of the infusion device of the present invention eliminates the need to use an dilator to dilate the cervix.

In addition, since the maximum diameter of the tip 34 is larger than the outer diameter of the cylindrical sheet 58 of the first pollution prevention means 42, the cylindrical sheet (when the tip 34 is moved forward in the cervix 2) The end of 58 does not damage the cervix 2.

Moreover, since the cylindrical sheet 58 of the first pollution prevention means 42 always surrounds the outer surface of the cylinder 30, the bacteria in the cervix 2 are inserted in the state where the cylinder 30 is inserted into the cervix 2. Or contamination of the cylinder 30 by foreign matters can be prevented.

Next, as shown in FIG. 20, the tip 34 is positioned at the inlet 6c of the uterine angle 6, that is, the tip 34 is placed by the cervical canal 2 by the hand placed in the rectum. When it is urged to be located at the deep part of the body or the inlet 4a of the uterine body 4, the insertion of the cylinder 30 is stopped, the piston 40 is advanced, and the semen loaded in the straw 38 is delivered to the cervical canal 2 If the injection into the deep or inlet of the uterine body (4) the artificial insemination work is completed. When the fertilized egg is implanted using the injection device of the first embodiment, that is, when the fertilized egg is implanted in the straw 38, the cylinder 30 is further advanced so that the tip 34 is extended to the tip 6a of the uterine angle 6. Inject the fertilized egg by inserting.

At this time, since the drain hole 52 formed in the tip 34 is formed smaller than the diameter of the straw 38, and the diameter of the discharge port 52a of the drain hole 52 is smaller than the diameter of the inlet 52b, the cap-shaped thin film Even if the tube 86 does not use the fixed second contamination prevention means 44, it is possible to minimize the mucus in the cervix entering the drain 52 when the tip 34 passes through the cervix 2. do.

After the injection of the germ cells is completed, the injection device is withdrawn from the germ bubble, since the tip 34 is firmly coupled to the cylinder 30 and the cylindrical sheet 58, the tip 34 is a cylinder in the withdrawal process It is not separated from the 30 or the cylindrical sheet 58.

Then, the injector is removed from the genital organs, and the contaminated auxiliary cylinder 66, the cylindrical sheet 58, the tip 34 and the straw 38 are contaminated as they pass through the vagina 8 and the cervix 2. If you separate them from them and replace them with new ones, you can continue to use them without disinfecting the injection device.

The injection device of the first embodiment described above is mainly used for artificial insemination of all mammals including cattle, but can also be used for fertilized egg implantation.

The second embodiment of the apparatus for injecting germ cells into the genitals of a mammal according to the present invention will be described with reference to FIGS. 12 to 17 as follows.

In the second embodiment, the same parts as in the first embodiment will be described using the same reference numerals, and the same modifications as in the first embodiment will be described with reference to the drawings of the first embodiment without showing a separate drawing.

The second embodiment of the injection device of the present invention is suitable for use in implantation of a fertilized egg in a mammal, but can also be used in artificial insemination. In the injection device of this embodiment, the body 46 of the tip 34 is formed long enough for implantation of the fertilized egg, inserting the support cylinder 102 to the outside of the cylinder 30 to which the tip 34 is fixed, The tip 34 differs from the injection device of the first embodiment in that it is configured to only support the tip 34 by the support cylinder 102 at the time of passage of the cervix. In addition, in this embodiment, the first pollution prevention means 42 is inserted outside the support cylinder 102 and has a structure surrounding the tip portion 48 of the tip 34.

As shown in FIG. 12, the tip 34 is fixed to the cylinder 30 via the adapter 74 as in the first embodiment, so that the tip 34 can be pulled out to the tip of the uterine angle during implantation of the fertilized egg. It is formed long (see FIG. 25). The support cylinder 102 is inserted outside the cylinder 30 so that its tip contacts the inclined intermediate portion 50 of the tip 34 to support the tip 34 and the body of the tip 34. 46) in it. A flange 104 is formed at the rear end of the support cylinder 102. In this embodiment, the first pollution prevention means 42 is a flexible tube inserted outside the tip 34 and the support cylinder 102 to prevent contamination of the tip 34 and the support cylinder 102. 106, the rear end of the tube 106 is supported in contact with the flange 104 of the support cylinder 102, the tip end of which surrounds the tip 34 and is indicated by reference numeral 108. The opening 110 of () is folded to close. The second pollution prevention means 44 is inserted outside the tube 106 of the first pollution prevention means 42, and the configuration thereof is the same as that of the first embodiment.

In the second embodiment as in the first embodiment, an O-ring 43 is provided between the cylinder 30 and the piston 40 to prevent the piston 40 from being freely separated from the cylinder 30.

As shown in FIG. 13, the tip 34 is generally cylindrical in cross section, and when the length of the tip 34 is short, a rigid body for adjusting the flexibility of the tip 34 can be embedded. Although not necessary, when the length of the tip 34 is long, it is preferable to embed the rigid body in the tip 34.

As shown in FIG. 14, at least one rigid body 112 is prevented to prevent the tip 34 from severely bending as it passes through the uterine angle when the body 46 of the tip 34 is long. It may be embedded in the body 46 of the tip 34. However, the rigid body 112 does not necessarily need to be embedded in the body 46 of the tip 34. The reason for this is that the length and shape of the tip 34 may be properly adjusted according to the size and shape of the genitals of various mammals. This is because the flexibility of the tip 34 can be adjusted by appropriately selecting the material of the tip 34 by changing the flexibility of the tip 34.

Fixing of the tip 34 and the cylinder 30 and the fixing of the tip 34 and the straw 38, and also the fixing of the tip 34 and the adapter in addition to those shown in FIG. Of course, one of the fixing structure shown in the figure can be used.

Since the length of the uterine angle is different according to the type of animal, especially in the case of fertilized egg transplantation of an animal having a long uterine angle, the length of the body 46 of the tip 34 should be as long as that. In this regard, as described in detail below, during implantation of the fertilized egg, the gap between the flange 104 of the support cylinder 102 and the flange 32 of the cylinder 30 and the flange of the auxiliary cylinder 66 is increased. Therefore, the means 114 which can hold each of these flanges with one hand is provided in the support cylinder 102.

As shown in FIG. 12, the gripping means 114 is movably inserted into the tube 106 of the contamination prevention means 42 and the flange 64 and the support cylinder 102 of the auxiliary cylinder 66. Of the support piece 116 located between the flange 104 of the support piece, the hole 118 of the flange 104 of the support cylinder 102 and the hole 120 of the hole support piece 116 of the support piece 116. And a gripping ring 124 fixed to both ends of the rod 122. The action of the gripping means 114 will be described in detail later.

FIG. 15 shows a variation of the gripping means 114. The rod of the gripping means 114 is constituted by a foldable antenna-shaped rod 126 in which gripping rings 124 are fixed at both ends. Therefore, the folding of the rod 126 makes it easy to carry the injection device and easily hold the cylinder 30, the auxiliary cylinder 66, and the support cylinder 102 even when the lengths of the uterine angles of various animals are different. Can be.

FIG. 16 shows a variation of the injection device shown in FIG. 12, wherein the injection device of this modification has no tube 106 of the first pollution prevention means 42 and the second pollution prevention shown in FIG. It is generally similar to the injection device of FIG. 12 except that it has a means 44. The tip 34 is also fixed in the annular recess 75 of the adapter 74.

The thin film cap 68 fixed to the tip of the second pollution prevention means 44 prevents contamination of the vagina and cervix inlet, and the cap type thin film tube 86 fixed in the middle of the first and second embodiments is the first and second embodiments. The function of the cylindrical sheet 58 or the tube 106, which is the anti-contamination means 42, is further enhanced, and the rupturable portion 94 prevents the tip 34 from rupturing while being inserted into the uterine body 4. It is formed in the center of the thin film tube 86. Advancement of the tip 34 causes the rupturable portion 94 of the cap-shaped thin film tube 86 to rupture first, and then the tip portion 48 of the tip 34 passes through the ruptured hole, thereby widening the tip 34 during the procedure. ) Is completely sterile so that it can be inserted into the uterine body (4) or uterine angle (6).

FIG. 17 shows a variation of the support cylinder 102 shown in FIG. 16, even when the tube 106 of the first pollution prevention means 42 is not used. Since it is in contact with the inclined intermediate portion 50 of 34, it may serve to prevent contamination of the vaginal and cervical canal of the cylinder 30. In this embodiment, in order to form a distal end of the support cylinder 102 in contact with the cervical canal 2 so that the contaminated site can be replaced with a sterile new one after use, the support cylinder 102 is formed in the first body. And a second body 130, which are detachably coupled to each other. In more detail, the coupling groove 132 is formed in one side of the second body 130 of the support cylinder 102 and the annular support groove 134 is formed on the inner circumferential surface of the coupling groove 132 to form the second groove 130. The front end of the first body 128 in the coupling groove 132 of the body 130 is fixed by fixing the first body 128 and the second body 130, but the annular support projection of the first body 128 136 is firmly coupled to the annular support groove 134. In addition, a cutout 138 is formed at the distal end of the first body 128 such that the distal end of the first body 128 is easily inserted into the coupling groove 132 of the second body 130. During the treatment by the injection device, the first body 128 of the support cylinder 102 is not contaminated by the second pollution prevention means 44, and only the second body 130 is contaminated. Therefore, only the second body 130 is new. You can change it. Thus, the injection device can be used repeatedly without disinfection.

According to the second embodiment of the present invention configured as described above, a process of implanting fertilized eggs into an animal using a device for injecting germ cells into a genital of a stray animal is as follows.

First, as shown in FIG. 21, the injection device in which the fertilized egg is loaded in the straw 38 is inserted into the vagina, and the thin film cap 68 fixed to the tip of the auxiliary cylinder 66 of the second pollution prevention means 44. The infusion device is inserted into the vagina 8 so that is located at the entrance of the cervix 2.

At this time, since the auxiliary cylinder 66 and the thin film cap 68 of the second pollution prevention means 44 surround the cylinder 30 and the tip 34, the cylinder 30 and the tip 34 are bacteria or foreign substances in the vagina. Contamination is prevented by

And the injection device is in contact with the flange 104 of the support cylinder 102, one flange 124 of the holding means 114 in a state where the thin film cap 68 is inserted so as to be positioned at the inlet of the cervix 2 At the same time, the support piece 116 is located close to the flange 64 of the auxiliary cylinder 66.

Next, insert the index finger and middle finger of the flange 64 of the auxiliary cylinder 66, and push the support piece 116 with the thumb, the tip of the support cylinder 102 is pushed forward while pushing the tip 34, the thin film cap When 68 is widened by its elasticity, pushing the support piece 116 further ruptures the thin film cap 68 of the second pollution prevention means 44 as shown in FIG. At this time, since the radial cut line 96 as shown in FIG. 10 of the first embodiment is formed in the thin film cap 68, the thin film cap is not broken or debris is generated. When the cap 44 is provided with a stretchable cap-shaped thin film tube 86 (see FIG. 9) and does not have the tube 106 of the first pollution prevention means 42, the thin film cap 68 When the support piece 116 is continuously pushed in this ruptured state, the thin film tube 86 directly contacts the cervical canal 2 while the tip 34 is accommodated in the thin film tube 86 to the cervical canal 2. When the tip 34 enters the middle portion of the cervix 2 or the uterine body 4, the tip portion 48 of the tip is rupturable due to the limitation of the length of the thin film tube 86. ) And the tip is further advanced so that the acid end of the tip 34 passes through the hole ruptured by the elongation of the thin film tube 86. The streamlined tip 48 of the tip 34 is then exposed in the sterile cervix 2 or uterine body 4. The tip 34 then enters the uterus in a sterile state).

Next, the tube 106 of the first pollution prevention means 42 surrounding the tip 34 is pulled out to be in direct contact with the cervix 2. As a result, the tube 106 of the first pollution prevention means 42 enters the cervical canal 2 while wrapping the streamlined tip portion 48 of the tip 34.

Then, in this state, as shown in FIG. 23, when the support cylinder 102 is continuously pushed into the cervical canal 2, the outer diameter of the tip 34 tip is made larger than the outer diameter of the support cylinder 102. FIG. As such, the support cylinder 102 easily passes through the cervix 2 while supporting the tip 34 in a flowable manner.

At this time, since the tip 34 is formed of a flexible flexible body and its tip portion is formed in a streamlined shape, the tip 34 is easily moved while bending itself along the internal wall of the cervix 2 having an irregular shape. 34) When passing through the cervix, the cervix (2) is not injured at all.

In addition, as shown in FIG. 23, after the support cylinder 102 passes through the cervix 2, the tube 106 folded at the inlet 6c of the widened uterine body 4 or the uterine angle 6 The opening 110 of the unfolded, the tip 34 is exposed to the uterine angle in a sterile state. Therefore, when the injection device passes through the cervix 2, the tip 34 and the support cylinder 102 are prevented from being contaminated by bacteria or foreign matter in the cervix 2.

And as shown in FIG. 23, the tip 34 and the support cylinder 102 pass through the cervical canal 2 to the inlet 6c of the uterine angle 6 on the side to which the tip 34 is to be implanted. Guided by the hand put into the rectum, the insertion of the support cylinder 102 is stopped, the flange 104 of the support cylinder 102 and the flange 32 of the cylinder 30 by narrowing the thumb and index finger to the cylinder The 30 is pushed out to start the extraction of the tip 34.

At this time, when the propulsion of the cylinder 30 with one hand is difficult because the flange 104 of the support cylinder 102 and the flange 32 of the cylinder 30 are wide, the support piece is advanced toward the auxiliary cylinder 66 ( 116 is brought into contact with the flange 104 of the support cylinder 102 and then pulled by the holding means 114 to move to the flange 32 side of the cylinder 30.

Next, when the cylinder 30 moves forward, the auxiliary cylinder 66 is placed so that the rectangular flange 64 of the auxiliary cylinder 66 in the vertical state does not interfere with the movement of the rod 122 of the gripping means 114. Rotate 90 degrees, hold your thumb on the flange 32 of the cylinder 30, the index finger and ring finger holding the flange 104 of the support cylinder 102, push the cylinder 30 toward the support cylinder 102 workability This makes it possible to operate the injection device with one hand, eliminating the need for an assistant and simplifying operation.

As shown in FIG. 24, when the cylinder 30 is continuously pushed, the tip 34 inserted into the support cylinder 102 is pulled out vertically while the streamlined tip portion 48 of the tip 34 is uterine angle. As the body 46 of the tip 34 is bent and advanced in the shape of the uterine angle 6 as it slides in contact with the inner surface of the 6, the streamlined tip portion of the tip 34 without damaging the uterine angle ( 48) is inserted up to the tip 6a of the uterine angle 6, the implantation site in the natural state of the fertilized egg.

At this time, if the operator touches the hand inserted into the rectum to the tip of the uterine angle (6), it is possible to feel the state that the tip of the tip 34 is guided to the tip (6a), which is the core of the uterine angle (6).

Next, as shown in FIG. 25, when the piston 40 is advanced with the streamlined tip 48 of the tip 34 positioned at the tip 6a of the uterine angle 6, the piston 40 ) Is inserted along the support jaw 36 of the cylinder 30 and inserted into the straw 38, and the fertilized egg loaded in the straw 38 is injected into the tip 6a, which is the core of the uterine angle 6 together with the medium. Done. At this time, since the drain hole 52 of the tip 34 is opened at the front of the streamlined tip portion 48, the injected fertilized egg is attached to the tip 34 so that it is not lost. Therefore, the conception rate by fertilized egg transplant is improved.

After implantation of the fertilized egg is completed, the injector is withdrawn from the genital tract. At this time, the tip 34 is firmly coupled to the cylinder 30 so that the tip 34 is not separated from the cylinder 30 during the extraction process. After the injector is removed, the tip 34 and straw 38 are removed at the same time as the contaminated auxiliary cylinder 66 and tube 106 are separated from the injector as it passes through the vagina 8 and the cervix 2. Remove it, replace it with a new one, and assemble it for use. Therefore, the injection device can be used repeatedly without disinfection separately.

In addition, when using the support cylinder 102 separated and formed by the 1st, 2nd body 128, 130 as shown in FIG. 17, without using the tube 106, Since only the second body 130 is removed and a new sterilized second body is used in combination with the first body 128, the first body 128 of the support cylinder 102 can be used continuously without disinfection. .

The injection device according to the second embodiment of the present invention is used for implanting embryos of mammals with well-developed uterine angles, but may also be used for implantation and artificial insemination of human embryos with poor development of uterine angles. In the case of artificial insemination, the semen loaded in the straw 38 may be injected into 2/3 of the cervix or the inlet 4a of the uterine body, so that the semen loaded straw 38 is fixed to the tip 34. In combination with the cylinder 30, the distal end of the injection device is inserted into the 2/3 point of the cervix or into the inlet 4a of the uterine body (see FIG. 23), and then the piston 40 is pushed in the semen in the straw 38. To discharge. In this case, the tip 34 may be discharged from the support cylinder 102 in a state in which the semen may be discharged, but the tip 34 may be discharged from the support cylinder 102 to discharge the semen according to the structure of the animal genitalia. .

As described above, according to the apparatus for injecting germ cells into the genitals of the mammal of the present invention, the insertion into the cervix can be easily performed, and the cervix and the cervix can be prevented from being damaged. It prevents contamination of the tip and cylinder by bacteria or foreign substances at the entrance and mucus in the cervix, thereby preventing contamination in the uterus and injecting fertilized eggs into the tip of the uterine angle, which is an implantation site in the natural state. In addition, the conception rate can be improved since the lost fertilized egg can be prevented, and since the fertilized egg is not arbitrarily separated in the assembled state, there is an advantage of workability and portability.

Claims (39)

A flange having a flange formed at one side, and a supporting jaw formed at an inner surface thereof, a straw inserted into the cylinder to be supported by the supporting jaw of the cylinder, and having a germ cell extended, and a leading end of the straw, A flexible tip fixed to the distal end of the cylinder, the streamlined distal end having a diameter larger than the diameter of the cylinder, a drainage tip communicating with the straw, and movably inserted into the cylinder, A piston which is guided along the support jaw forcibly discharging germ cells loaded on the straw through the drain hole of the tip, and locking means installed between the cylinder and the piston to prevent the piston from flowing; A first pollution prevention means installed on the outside of the cervix and preventing contamination of the cylinder by the cervix during passage of the cervix; Installed on the outer side of the first pollution prevention means in a state where the tip is wrapped, and includes a first pollution prevention means by the vagina when passing through the vagina and a second pollution prevention means for preventing contamination of the tip. Device for injecting germ cells. The device of claim 1, wherein the flexible tip comprises a body, an intermediate portion, and the streamlined tip. The apparatus for injecting germ cells into the genitals of a mammal according to claim 1, wherein the outlet diameter of the drain port is smaller than the inlet diameter. The device of claim 1, wherein the distal end of the cylinder is screwed to the body of the tip. According to claim 1, wherein the tip is coupled to the straw by the adapter, the inner surface is screwed to the outer peripheral surface of the adapter to the front end of the cylinder, the adapter has a through hole communicating with the drain and the drain hole of the tip Apparatus for injecting germ cells into the genitals of a mammal, characterized in that provided. 6. The device of claim 5, wherein the adapter has a capillary tube inserted into and fixed in the drainage port of the tip. The mammalian genital organ according to claim 1, wherein the first pollution prevention means is formed in a cylindrical sheet inserted into an outer side of the cylinder and in contact with an inclined middle portion of the tip to support the tip. Device for injecting germ cells. 6. The method of claim 5, wherein the first pollution prevention means is integrally fixed by fusion fixing the outer surface of the tip body and the outer surface of the adapter to the inner circumferential surface of the tip of the cylindrical sheet so as to be inserted into the outer side of the cylinder and assembled with the cylinder by rotation. Apparatus for injecting germ cells into the genitals of mammals characterized in that. According to claim 1, wherein the first pollution prevention means, the cylindrical sheet is inserted into the outer side of the cylinder, is installed on the outer surface of the cylinder, so that the front end portion of the cylindrical sheet is elastically in contact with the inclined middle portion of the type Apparatus for injecting germ cells into the genitals of a mammal, characterized in that it comprises a spring for pressing the cylindrical sheet toward the tip, and a cylindrical holder fixed to the outer surface of the cylinder, surrounding the spring. The reproductive system of claim 9, wherein the gripping ring is movable on the outer surface of the cylindrical holder, and an annular protrusion is formed on the cylindrical holder to prevent the gripping ring from being separated. Device for injecting cells. The method of claim 7 or 8, wherein the second pollution prevention means is inserted into the outer side of the cylindrical sheet and the tip, the auxiliary cylinder having a rectangular flange formed on one side, and is fixed to the front end of the auxiliary cylinder, Apparatus for injecting germ cells into the genitals of a mammal, characterized in that the thin film cap is ruptured by the advance of the tip. 12. The apparatus of claim 11, wherein a plurality of radial cut lines are formed in the thin film cap. 13. The method of claim 12, wherein the second pollution prevention means further comprises a stretchable cap-type thin film tube positioned between the tip and the thin film cap and fixed to the tip of the auxiliary cylinder, and having a tearable portion formed in the center thereof. Apparatus for injecting germ cells into the genitals of mammals, characterized in that. 15. The device of claim 13, wherein the annular protruding ring of the stretchable cap-shaped thin film tube is fixed to the tip of the auxiliary cylinder by a fixing ring. 2. The device of claim 1, wherein the locking means is an O-ring installed in the recess of the piston. The device of claim 1, wherein the locking means comprises a ring-shaped leaf spring formed on the inner surface of the cylinder and pressurizing the outer surface of the piston. A flange having a flange formed at one side, and a supporting jaw formed at an inner surface thereof, a straw inserted into the cylinder to be supported by the supporting jaw of the cylinder, and having a germ cell extended, and a leading end of the straw, A flexible tip fixed to the distal end of the cylinder, the streamlined distal end having a diameter larger than the diameter of the cylinder, a drainage tip communicating with the straw, and movably inserted into the cylinder and supporting the cylinder. A piston for guiding discharge of the germ cells, which are guided along the jaw and loaded into the straw through the drain hole of the tip, and locking means installed between the cylinder and the piston to prevent flow of the piston; A support cylinder inserted into an outer side to deform the tip and having a flange formed on one side thereof, and the support It is installed on the outside of the linder and the tip, the first pollution prevention means for preventing their contamination by the cervix when passing through the cervix, and is installed outside the first pollution prevention means with the tip wrapped, Apparatus for injecting germ cells into the genitals of a mammal comprising a first pollution prevention means by the vagina when passing through the vagina and a second pollution prevention means for preventing contamination of the tip. 18. The device of claim 17, wherein the tip comprises an elongated flexible body, an intermediate portion and the streamlined tip. 19. The device of claim 18, wherein the distal end of the cylinder is screwed into the body of the tip. 19. The method of claim 18, wherein the tip is coupled to the straw by an adapter, the inner surface of the front end of the cylinder is screwed to the outer peripheral surface of the adapter, the adapter has a through hole in communication with the drain and the drain hole of the tip Apparatus for injecting germ cells into the genitals of a mammal, characterized in that one. 19. The device of claim 18, wherein at least one rigid body for embedding flexibility is embedded in the body of the tip. 18. The mammal according to claim 17, wherein the first pollution prevention means is made of a flexible tube inserted into an outer side of the support cylinder and the tip and having a tip opening folded to surround the streamlined tip of the tip. Device for injecting germ cells into the genitals. The auxiliary cylinder according to claim 22, wherein the second pollution prevention means is inserted into an outer surface of the flexible tube while the flexible tube surrounds the streamlined tip of the tip, and has an auxiliary cylinder having a rectangular flange formed on one side thereof. Apparatus for injecting germ cells into the genitals of a mammal, characterized in that it comprises a thin film cap fixed to the tip of the auxiliary cylinder. 24. The apparatus of claim 23, wherein a plurality of radial cut lines are formed in the thin film cap. 18. The device of claim 17, wherein said locking means is an O-ring installed in the recess of said piston. 18. The apparatus of claim 17, wherein the locking means comprises a ring-shaped leaf spring formed on the inner side of the cylinder and pressurizing the outer side of the piston. 24. The apparatus for injecting germ cells into a genital genital mammal according to claim 23, wherein gripping means for gripping the flange of the cylinder and the rectangular flange of the auxiliary cylinder is provided on the flange of the support cylinder. 28. The holding member according to claim 27, wherein the holding means is movably installed in the support piece movably inserted into the outer surface of the flexible tube of the second pollution prevention means, in the hole of the flange of the support cylinder and the hole of the support piece. Apparatus for injecting germ cells into the genitals of a mammal, characterized in that the rod having a gripping ring. 29. The device of claim 28, wherein said rod has a collapsible antenna structure. A flange having a flange formed at one side, and a supporting jaw formed at an inner surface thereof, a straw inserted into the cylinder to be supported by the supporting jaw of the cylinder, and having a germ cell extended, and a leading end of the straw, A flexible tip fixed to the distal end of the cylinder, the streamlined distal end having a diameter larger than the diameter of the cylinder, a drainage tip communicating with the straw, and movably inserted into the cylinder and supporting the cylinder. A piston which is guided along the jaw and forced to discharge the germ cells loaded in the straw through the drain hole of the tip, a locking means installed between the cylinder and the piston to prevent the piston from flowing, and an outer side of the cylinder. A support cylinder inserted into and deformably supporting the tip and having a flange formed at one side thereof; It is installed on the outside of the support cylinder in a wrapped state and prevents contamination of the tip, and is inserted into the outside of the support cylinder, and has an auxiliary cylinder having a rectangular flange formed on one side, and a tip portion of the auxiliary cylinder. A stretchable cap-shaped thin film tube having a fixed thin film cap and a breakable portion fixed to the tip of the auxiliary cylinder to surround the tip and located inside the thin film cap and rupturing in advance of the streamlined tip of the tip. Apparatus for injecting germ cells into the reproductive system of the mammal comprising the anti-fouling means having a; and a gripping means installed on the flange of the support cylinder for gripping the flange of the cylinder and the auxiliary cylinder. 31. The device of claim 30, wherein the tip comprises an elongated flexible body, an intermediate portion, and the streamlined tip. 32. The device of claim 31, wherein at least one rigid body for embedding flexibility is embedded in the body of said tip. 31. The method of claim 30, wherein the tip is coupled to the straw by an adapter, the inside of the tip of the cylinder is screwed with the outer circumferential surface of the adapter, and the adapter has a through hole communicating with the drain and the drain hole of the tip. Apparatus for injecting germ cells into the genitals of mammals, characterized in that. 34. The apparatus of claim 33, wherein the adapter has a capillary tube inserted into and fixed in the drain hole of the tip. 34. The apparatus of claim 33, wherein a plurality of radial cut lines are formed in the thin film cap. 31. The device of claim 30, wherein the locking means is an O-ring installed in the recess of the piston. 31. The device of claim 30, wherein the locking means comprises a ring-shaped leaf spring formed on the inner side of the cylinder and pressurizing the outer side of the piston. 38. The holding member according to claim 36 or 37, wherein the holding means is movably provided in a support piece movably inserted into an outer surface of the support cylinder, a hole in a flange of the support cylinder, and a hole in the support piece. Apparatus for injecting germ cells into the genitals of a mammal, characterized in that the rod having a gripping ring. 39. The device of claim 38, wherein said rod has a collapsible antenna structure.