JP4769913B2 - Deep injector for transplantation of livestock embryos - Google Patents

Description

  The present invention relates to a device for transplanting an embryo fluid such as a fertilized egg of a domestic animal such as a cow or a pig, and more particularly, directly from a straw tube in which an embryo fluid such as a fertilized egg is cryopreserved without deeply damaging the uterus of the domestic animal. The present invention relates to a deep implanter for transplanting livestock embryos that can be transplanted into a plant.

Artificial insemination is usually performed as a breeding technique for cattle, for example, for livestock, but this is an improvement from the male side, whereas the fertilized egg transplantation technique collects fertilized eggs from artificial cows after artificial insemination. Since this is transplanted to the recipient cow, it is a technique that can be improved from both males and females, and the efficiency of the improvement can be improved.
However, there is a difference in conception rate among transplantation engineers in fertilized egg transplantation, and the uterus stimulation at the time of transplantation and the difference in transplantation site are considered as the personnel.
Currently, the straight sheath tube type transplanter, which is often used as a transplanter for fertilized eggs, is difficult to transplant to the bent deep uterus, stimulates the endometrium when inserted into the uterus, There is concern about hurting.
In order to improve these problems, a transplanter using a flexible tube (Japanese Examined Patent Publication No. 61-36935) has been developed. In this case, a fertilized egg filled in a straw tube is previously filled in the flexible tube. It must be repaired and the transplanter must be cleaned and sterilized each time it is used.
Therefore, as a solution to the above-described drawbacks, a transplantation device (Japanese Patent Laid-Open No. 2001-120581) that can be transplanted without refilling a fertilized egg from a straw tube into a flexible tube has been developed. This has a structure in which a straw tube is inserted into a flexible inner tube corresponding to a flexible tube (see FIG. 7).
Shoko 61-36935 JP2001-120581A

  However, in the transplantation device disclosed in Japanese Patent Application Laid-Open No. 2001-120581, the straw tube inserted into the flexible inner tube is a cryopreserved fertilized egg. A flexible material cannot be used and is made of a slightly harder material. For this reason, the flexible inner tube is less flexible due to the slightly stiff straw tube inserted inside it, making it difficult to bend inside the uterus, resulting in irritation and further damage to the endometrium Is concerned.

  The present invention has been made in view of the above problems, and has been devised to solve the problems. The object of the present invention is directly from a straw tube in which embryo fluid such as a fertilized egg is frozen and stored. It is an object of the present invention to provide a deep injector for transplanting livestock embryos that can be transplanted deep without damaging the uterus.

In order to achieve the above object, the present invention includes a flexible tube that has a pouring tip at the tip and is pushed out from the tip of the hard shell in the uterus inside the hard shell that is open at both front and rear ends. Is connected to the rear end of the flexible tube, and a hard inner cylinder protruding rearward from the rear end of the hard outer cylinder is movably inserted. Insert the straw tube containing the germ fluid into the inner tube from the rear end of the hard inner cylinder, and enter the germ fluid in the straw tube from the rear end of the straw tube into the distal end and the distal end of the flexible tube into the flexible tube. It comprises means for inserting an extrusion rod, which is pushed into the flexible tube through a stopper moving from the inner rear end toward the front end, from the rear end of the hard inner cylinder.

As is clear from the above description, the deep implanter for transplanting livestock embryos according to the present invention has the following extremely novel and beneficial effects.
(1) Compared to a normal linear transplanter, the possibility of damaging the uterus is low, and transplantation can be performed deep in the uterus.
(2) Transplantation through a flexible tube is possible without refilling the germ fluid packed in an existing straw tube.
(3) When transplanting through the flexible tube, the existing slightly stiff straw tube is stopped inside the hard outer tube and the hard inner tube, and is not inserted into the flexible tube. Is inserted into the inside of the flexible tube so that the flexibility is lowered and it is difficult to bend inside the uterus.
(4) Since uterine damage is reduced and deep transplantation is possible, an improvement in conception rate can be expected.
(5) Since this deep injector for transplantation is disposable except for the extrusion rod, it is hygienic.

  Hereinafter, the present invention will be described more specifically based on the best mode for carrying out the invention described in the drawings.

  In the figure, a deep implanter 1 for transplanting livestock embryos is an instrument that can be transplanted directly from a straw tube 5 in which embryo fluid a such as a fertilized egg is cryopreserved without deeply damaging the uterus of livestock. 2. The flexible tube 3 and the hard inner cylinder 4 inserted in the hard outer cylinder 2, the straw tube 5 inserted in the hard inner cylinder 4, and the germ fluid a in the straw tube 5 It is comprised from the extrusion rod 6 etc. which are extruded inside.

  The hard outer cylinder 2 is made of, for example, a hard and slender cylindrical tube, and for example, a stainless tube is used. The front and rear ends of the hard outer cylinder 2 are opened. At the time of transplantation, the hard outer cylinder 2 is inserted to the vicinity of the cervical outer mouth of a domestic animal such as a cow. The hard outer cylinder 2 has a flexible tube 3 and a hard inner cylinder 4 inserted therein. Further, a straw tube 5 containing an embryo fluid a is inserted into the hard inner cylinder 4.

  The hard outer cylinder 2 is made of a hard material so as not to be bent easily by its own weight or when hitting the inner wall or the like when inserted toward the womb of the cow. The hard outer cylinder 2 also functions to protect the flexible tube 3, the hard inner cylinder 4, the straw tube 5, and the like inserted inside during transplantation from being clogged or deformed in the middle.

  The flexible tube 3 and the hard inner cylinder 4 inserted into the hard outer cylinder 2 are movable toward the tip. That is, the inner diameter of the hard outer cylinder 2 is slightly larger than the outer diameters of the flexible tube 3 and the hard inner cylinder 4, and the flexible tube 3 and the hard inner cylinder 4 smoothly move toward the tip from the inside of the hard outer cylinder 2. Can be moved to.

  The inner diameter of the distal end is slightly larger than the outer diameter of the flexible tube 3 so that the flexible tube 3 inserted into the rigid outer cylinder 2 is smoothly pushed out from the distal end of the rigid outer cylinder 2. Further, the flexible tube 3 pushed out from the tip has a structure in which the rear end does not protrude from the inside of the hard outer tube 2, and the tip of the hard inner tube 4 to which the rear end of the flexible tube 3 is connected is hard outer. The tube 2 is prevented from coming out from the tip.

  The flexible tube 3 is inserted into the distal end side of the hard outer cylinder 2, and when the distal end of the inserted hard outer cylinder 2 passes through the cervical canal and reaches the uterus, the flexible tube 3 is pushed out from the distal end of the hard outer cylinder 2. This is the part that is inserted into the womb. The flexible tube 3 is made of a soft material that is easy to bend and does not damage the uterus.

  The flexible tube 3 has, for example, an elongated cylindrical shape, and the embryo fluid a packed in the straw tube 5 is transplanted into the uterus through the flexible tube 3. The inner diameter of the flexible tube 3 is the same as the inner diameter of the straw tube 5 so that the germ solution a in the straw tube 5 can be easily filled into the flexible tube 3.

  A pouring tip 31 is provided at the tip of the flexible tube 3. The dispensing tip 31 is rounded so as not to damage the inner wall of the uterus. Further, an extraction hole 32 is formed at the tip of the flexible tube 3 provided with the extraction tip 31, and the embryo fluid a in the flexible tube is transplanted into the uterus from the extraction hole 32.

  At the rear end of the flexible tube 3, when the germ fluid a in the straw tube 5 is pushed out into the flexible tube 3, the tip of the straw tube 5 is abutted and locked so as not to enter the inside. A stopper 33 is formed on the periphery of the opening. This stopper 33 prevents the straw tube 5 from being inserted into the flexible tube 3.

  The hard inner cylinder 4 is made of, for example, a hard and slender cylindrical tube, and for example, a stainless tube is used. The front and rear ends of the hard inner cylinder 4 are opened. The hard inner cylinder 4 is inserted therein with a straw tube 5 containing the germ fluid a. The front end of the hard inner cylinder 4 is connected to the rear end of the flexible tube 3, and the hard inner cylinder 4 and the flexible tube 3 are integrated and interlocked.

  The hard inner cylinder 4 has a structure in which the flexible tube with the tip connected is pushed out from the tip of the hard outer cylinder 2 by pushing the rear end side of the hard inner cylinder 4. For this reason, in order to avoid that the pushing force is not transmitted to the flexible tube 3 by bending or deforming inside the hard outer cylinder 2, it is made of a hard material. The rigid inner cylinder 4 also functions to protect the straw tube 5 inserted therein from being clogged or deformed.

  The hard inner cylinder 4 whose front end is connected to the flexible tube 3 has a rear end protruding outside the rear end of the hard outer cylinder 2. The rear end side of the hard inner cylinder 4 protruding to the outside is a portion that is press-fitted toward the hard outer cylinder 2 when the flexible tube 3 is pushed out from the tip of the hard outer cylinder 2. In addition, a stopper that contacts the rear end of the hard outer cylinder 2 may be provided on the rear end side in order to prevent the extruded flexible tube 3 from completely protruding from the tip of the hard outer cylinder 2.

  The straw tube 5 contains an embryo fluid a to be transplanted into the uterus. The straw tube 5 is inserted into the hard inner cylinder 4 from its rear end. As described above, the straw tube 5 is locked by contacting the rear end of the flexible tube 3 so as not to enter the flexible tube 3. The outer diameter of the straw tube 5 is slightly smaller than the inner diameter of the hard inner cylinder 4 so that the inside of the hard inner cylinder 4 can move smoothly toward the tip.

  As described above, the straw tube 5 is obtained by cryopreserving, for example, a fertilized egg as the embryo liquid a, and is made of a slightly hard material so as to withstand cryopreservation. For this reason, in order to avoid the flexibility of the flexible tube 3 being lowered by the slightly hard straw tube 5 inserted into the flexible tube 3 and becoming difficult to bend inside the uterus, as described above, The straw tube 5 cannot be inserted into the flexible tube 3.

  The straw tube 5 filled with the germ fluid a has, for example, an elongated cylindrical shape, and both the front and rear ends thereof are open, and the rear end side that is the opposite side of the front end of the straw tube 5 that contacts the flexible tube 3 A plug 51 is inserted in the interior of the. For example, a cotton plug is used as the plug 51. The plug 51 made of a cotton plug has a structure in which the inside of the straw tube 5 filled with the embryo fluid a is pushed out by the push rod 6 from the rear end to the front end.

  When the plug 51 is pushed out toward the tip by the push rod 6, the plug 51 pushes the germ fluid a packed in the straw tube 5 into the flexible tube 3, and further the flexible tube 3. The germ fluid a that enters the inside from the rear end and is pushed into the flexible tube 3 is pushed out into the uterus from the dispensing hole 32 on the tip side.

  The push rod 6 is inserted from the rear end of the hard inner cylinder 4 and pushes and moves a plug 51 made of a cotton plug whose tip is packed in the rear end of the straw tube 5 toward the tip of the straw tube 5. It is. The rear end side of the push bar 6 protrudes to the outside of the hard inner cylinder 4, and a handle or the like that is easy to operate is formed on the rear end side.

  The push rod 6 pushes the germ fluid a in the straw tube 5 into the flexible tube 3 through the plug 51, and further pushes and moves the plug 51 pushed into the flexible tube 3 toward the distal end side, It fulfills the function of transplanting the germ fluid a into the uterus from the tip-side dispensing hole 32 through a stopper 51 that is pressed and moved inside the straw tube 5 and the flexible tube 3.

  Thus, the distal end side of the push rod 6 is inserted through the inside of the straw tube 5 and enters the flexible tube 3 to press and move the plug 51 toward the distal end side. The flexible tube 3 is prevented from being bent easily by the distal end side of the inserted extrusion bar 6.

Next, a transplantation method based on the configuration of the best mode for carrying out the invention will be described below.
A flexible tube 3 and a hard inner cylinder 4 whose tip is connected to the rear end are inserted into the hard outer cylinder 2 from the front end of the hard outer cylinder 2. When the rigid inner tube 4 connected to the rear side of the flexible tube 3 and the flexible tube 3 is pushed in from the front end of the hard outer tube 2, the flexible tube 3 and the hard inner tube 4 smoothly move inside the hard outer tube 2. Move towards the rear edge. The distal end of the flexible tube 3 stops when it reaches the distal end of the hard outer cylinder 2, and the flexible tube 3 inserted into the hard outer cylinder 2 has only the distal end of the pouring tip 31 outside the hard outer cylinder 2. Protruding.

  In parallel with this operation, the straw tube 5 containing the embryo fluid a such as a fertilized egg is inserted into the hard inner cylinder 4. The straw tube 5 is inserted from the rear end of the hard inner cylinder 4. The straw tube 5 is inserted using the push rod 6. That is, after putting the straw tube 5 at the rear end of the hard inner cylinder 4, the stopper 51 packed in the rear end of the straw pipe 5 is pushed by the push rod 6, so that the entire straw tube 5 is advanced through the inside of the hard inner cylinder 4. Move towards.

  Thus, after inserting the flexible tube 3 and the hard inner cylinder 4 into the inside of the hard outer cylinder 2 and inserting the straw tube 5 containing the germ fluid a into the inside of the hard inner cylinder 4, The outer tube 2 is inserted so that the distal end side reaches the inside of the womb of a cattle such as a cow. In this case, insert with care not to damage the inner wall of the cow.

  When the distal end side of the hard outer tube 2 passes through the cervical canal and reaches the uterus, the insertion of the hard outer tube 2 is stopped and the flexible tube 3 is pushed out from the distal end of the hard outer tube 2. The rear end side of the hard inner cylinder 4 connected to the flexible tube 3 is pushed toward the inside of the hard outer cylinder 2. Since the flexible tube 3 and the hard inner cylinder 4 are interlocked and connected, when the rear end side of the hard inner cylinder 4 is pushed toward the hard outer cylinder 2, the flexibility inserted into the hard outer cylinder 2 is inserted. The tube 3 and the hard inner cylinder 4 are moved while being pressed toward the distal end side of the hard outer cylinder 2 as a whole. The distal end of the flexible tube 3 that moves by being pressed is pushed out from the distal end of the hard outer cylinder 2.

  The flexible tube 3 pushed out from the distal end of the hard outer cylinder 2 is inserted deep in the uterus. Since the flexible tube 3 inserted into the uterus is flexible, it is inserted deeply while bending along the uterus and does not damage the inner wall of the uterus. Further, since the slightly hard straw tube 5 is not inserted into the flexible tube 3, there is no possibility that the flexibility of the flexible tube 3 is impaired by inserting the straw tube 5.

  In this way, after the flexible tube 3 is pushed out from the tip of the hard outer cylinder 2, the rear end side of the push rod 6 is pushed toward the hard inner cylinder 4. When the push rod 6 is pushed in, the tip of the push rod 6 hits the plug 51 on the rear end side of the straw tube 5 inserted on the tip side of the hard inner cylinder 4, and the plug 51 is pushed and moved toward the tip of the straw tube 5. Let The germ fluid a is placed inside the straw tube 5, and the germ fluid a inside is pressed and moved toward the tip of the straw tube 5 by the stopper 51.

  The germ fluid a that has been pushed and moved by the push rod 6 through the stopper 51 toward the tip of the straw tube 5 is gradually pushed into the flexible tube 3 from the tip. After the germ fluid a in the straw tube 5 is pushed out and enters the flexible tube 3, the plug 51 in the straw tube 5 is also pushed out and enters the flexible tube 3.

  The germ solution a and the stopper 51 pushed into the flexible tube 3 by the push rod 6 are inserted into the flexible tube 3 by pushing the stopper 51 further into the flexible tube 3. For example, a fertilized egg is ejected into the deep part of the uterus together with the germ solution a from the dispensing hole 32 on the distal end side of the flexible tube 3 through the distal end side through 51, and transplantation is performed.

  Note that the present invention is not limited to the best mode for carrying out the invention, and various modifications can be made without departing from the spirit of the invention.

  Although explained in the case of cattle as livestock, it is also applicable to pigs. Moreover, although it demonstrated in the case of a fertilized egg as an embryo liquid, it is applicable also in the case of a sperm.

1 is a sectional view of a deep injector for transplantation of livestock embryos showing the best mode for carrying out the present invention. (A) is sectional drawing before extruding the flexible tube of the deep injection device for transplantation of the embryo for livestock which shows the best form for implementing this invention. (B) is sectional drawing when the flexible tube of the deep injector for transplantation of the embryo for livestock which shows the best form for implementing this invention is extruded. (C) is sectional drawing when the flexible tube of the deep injection device for transplantation of the embryo for livestock which shows the best form for implementing this invention is extruded in the uterus. (D) is sectional drawing when an embryo liquid is extruded from the flexible tube of the deep injector for transplantation of the embryo for livestock which shows the best form for implementing this invention. It is a figure when the deep injection device for transplantation of the embryo for livestock which shows the best form for implementing this invention is made to reach the cervical extraoral part. It is a figure when the deep injector for transplantation of the embryo | germ for livestock which shows the best form for implementing this invention is passed through a cervical canal and it is inserted to the middle uterine horn. Insert the flexible tube of the deep injector for transplantation of livestock embryo showing the best mode for carrying out the invention to a deep position of the uterine horn, push the embryo fluid together with the stopper and push the flexible tube part, It is a figure when inject | pouring.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Deep injector for transplant of livestock embryo 2 Hard outer cylinder 3 Flexible tube 31 Dispensing tip 32 Discharge hole 33 Stopper 4 Hard inner cylinder 5 Straw tube 51 Plug 6 Extrusion stick a Embryo

Claims (1)

A flexible tube that has a pouring tip at the tip and is pushed out from the tip of the hard tube in the womb, and a tip that is connected to the rear end of the flexible tube inside the hard outer tube that is open at both front and rear ends. A hard inner cylinder that protrudes outward from the rear end of the hard outer cylinder is movably inserted, and a straw tube containing embryo liquid that is locked with the distal end abutting on the rear end of the flexible tube is inserted into the hard inner cylinder. A plug that is inserted from the rear end into the inside, and the germ fluid in the straw tube enters the distal end from the rear end in the straw tube and enters the rear end in the flexible tube from the distal end and moves from the rear end to the front side in the flexible tube. A deep injector for transplanting livestock embryos, wherein an extruding rod to be pushed into the flexible tube is inserted into the inside from the rear end of the hard inner cylinder.

Images