JP2001089497A - Food containing supernatant fraction, water-insoluble fraction and monohydric alcohol extracted fraction of broccoli, cauliflower and kale - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a food effective for improving the environment in the body. SOLUTION: The present invention relates to a body-environment improving food containing at least one kind of fraction selected from supernatant fraction, monohydric alcohol extracted fraction and water-insoluble fraction of at least one kind of vegetable selected from broccoli, cauliflower and kale; a body- environment improving food containing a supernatant fraction and monohydric alcohol extracted fraction of at least one kind of vegetable selected from broccoli, cauliflower and kale; and a body-environment improving food containing a water-insoluble fraction and monohydric alcohol extracted fraction of at least one kind of vegetable selected from broccoli, cauliflower and kale.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a water-soluble fraction, a water-insoluble fraction and a monohydric alcohol extract fraction of broccoli, cauliflower and kale.

[0002]

2. Description of the Related Art In recent years, attention has been paid to the field of food science, and various edible plants have been studied. However, the present inventor has found that broccoli has an effect of lowering cholesterol levels and the like. I was doing research focusing on that.

[0003]

SUMMARY OF THE INVENTION An object of the present invention is to provide a food having an effect of improving the body environment.

[0004]

In studying the action of broccoli and the like, the present inventors also evaluated the supernatant fraction, the water-insoluble fraction, and the monohydric alcohol-extracted fraction. As a result, surprisingly, the supernatant fraction, the water-insoluble fraction and the monohydric alcohol extract fraction exhibited a better bile acid excretion promoting action and LDL- than the action of unfractionated broccoli. The present inventors have found that they have a cholesterol level lowering action, a neutral fat level lowering action, and a bifidobacterium growth promoting action, and completed the present invention.

That is, the present invention provides the invention according to the following items.

Item 1 A food for improving the body environment, comprising at least one of a supernatant fraction, a monohydric alcohol extract fraction and a water-insoluble fraction selected from the group consisting of broccoli, cauliflower and kale. .

Item 2 An environment-improving food containing at least one supernatant fraction and a monohydric alcohol extract fraction selected from the group consisting of broccoli, cauliflower and kale.

Item 3. An in-vivo environment-improving food containing at least one water-insoluble fraction and a monohydric alcohol-extracted fraction selected from the group consisting of broccoli, cauliflower and kale.

Item 4 The improvement in the body environment is at least one action selected from the group consisting of a bile acid excretion promoting action, a neutral fat lowering action, an LDL-cholesterol lowering action, and a bifidobacterium growth promoting action. Items 1-3
Food according to any of the above.

[0010] The improvement of the body environment of the present invention has the effect of promoting the excretion of bile acids, the effect of lowering triglyceride levels, the effect of LDL-
It refers to purifying the body (for example, purifying blood, improving the environment of the intestine) and the like by the action of lowering the cholesterol level and the action of promoting the growth of bifidobacteria, and further includes the effects expected from these actions. Such effects include, specifically, a preventive effect of hyperlipidemia, a preventive effect of hypercholesterolemia, a preventive effect of hypertriglyceridemia, a prevention of obesity, a prevention of hypertension, and a prevention of arteriosclerosis. , Improvement of intestinal flora, improvement of convenience, intestinal regulation, immunity activation and the like.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail.

The present invention relates to a supernatant fraction, a water-insoluble fraction and a monohydric alcohol extract fraction of broccoli, cauliflower and kale.

Hereinafter, broccoli, cauliflower and kale may be referred to as "vegetables of the present invention". Further, the supernatant fraction of broccoli, cauliflower and kale, the water-insoluble fraction and the monohydric alcohol extract fraction may be referred to as "fraction of the present invention".

Preparation method of supernatant fraction and water-insoluble fraction The supernatant fraction and water-insoluble fraction of broccoli, cauliflower and kale can be prepared by known methods. An example is shown below.

First, the vegetables of the present invention are washed, sterilized by heating if necessary, and then crushed to have a suitable size to obtain a crushed product. The crushing may be performed using a machine such as a Comitrol or a mass colloider. Although the degree of crushing is not particularly limited, it is usually crushed to a puree-like degree.

As the crushed material, commercially available (frozen) puree may be used. The frozen puree can be thawed at a low temperature of about room temperature or lower, or can be thawed by adding water and stirring.

Alternatively, after the freeze-dried crushed vegetable of the present invention, the freeze-dried product can be used for preparing a supernatant and a water-insoluble fraction.

Next, the crushed material itself or water is added to the crushed material and centrifuged. When adding water, about 0.5 to 10 L of water is usually added to 1 kg of the pulverized material. The conditions for centrifugation are not particularly limited,
It can be set appropriately according to the degree of pulverization, etc.,
Usually, it is performed at about 1,000 to 30,000 rpm for about 1 to 30 minutes.

After centrifugation, the supernatant and the precipitate (water-insoluble fraction) are separated.

Further water was added to the precipitate, and centrifugation was
It may be repeated about twice, in which case the resulting supernatant may be combined with the supernatant obtained in the first centrifugation.

Alternatively, the crushed material may be filtered using a filter cloth or the like, and the filtrate may be subjected to centrifugation.

The supernatant (water-soluble fraction) and precipitate (water-insoluble fraction) thus obtained may be used as they are,
It may be dried by hot air drying, freeze drying or the like and used as a dried product. Dried products are preferred because they mask bitterness and are easier to ingest than liquid or fluid water-soluble or water-insoluble fractions. The water-soluble fraction may be concentrated and used as a concentrated solution.

Method for Preparing Monohydric Alcohol Extraction Fraction The monohydric alcohol extraction fraction of broccoli, cauliflower and kale can be prepared by a known method. An example is shown below.

The crushed product can be obtained in the same manner as described in the method for preparing the water-soluble fraction.

Next, a monohydric alcohol is added to the crushed product and centrifuged. Here, pure monohydric alcohol may be used, or a mixture of monohydric alcohol and water may be used. When a mixture of a monohydric alcohol and water is used, the mixing ratio thereof is not particularly limited, but the monohydric alcohol is usually about 10 to 70% by weight. Hereinafter, unless otherwise specified, the “monohydric alcohol” includes a monohydric alcohol and a mixture of the monohydric alcohol and water. Here, as the monohydric alcohol, for example, ethanol,
Although propanol and n-butanol can be used, it is preferable to use ethanol.

The monohydric alcohol is usually added in an amount of about 0.5 to 10 L per 1 kg of the pulverized material. The operation of centrifugation is
It can be carried out in the same manner as described in the method for preparing the water-soluble fraction and the water-insoluble fraction.

The monohydric alcohol-extracted fraction thus obtained may be used as it is, or may be dried by hot-air drying, freeze-drying, etc. and used as a dried product. Dried products are preferred because bitterness is masked compared to liquid or fluid monohydric alcohol extraction fractions, and they are easy to ingest. The monohydric alcohol extraction fraction may be concentrated according to a conventional method and used as a concentrated liquid.

In the present invention, among these fractions, broccoli and cauliflower supernatant fractions, water-insoluble fractions and monohydric alcohol extraction fractions are preferred.

The broccoli supernatant fraction, cauliflower supernatant fraction and kale supernatant fraction may be used alone or in combination of two or more. The water-insoluble fraction of broccoli, the water-insoluble fraction of cauliflower and the water-insoluble fraction of kale may be used alone or in combination of two or more. Similarly, the monohydric alcohol extract fraction of broccoli, the monohydric alcohol extract fraction of cauliflower and the monohydric alcohol extract fraction of kale are also:
They may be used alone or in combination of two or more.

The vegetable supernatant fraction, the water-insoluble fraction and the monohydric alcohol extract fraction of the present invention may be used alone, and the supernatant fraction and the monohydric alcohol extract fraction or the water-insoluble fraction And the monohydric alcohol extraction fraction can be used in combination.

The supernatant fraction of broccoli, cauliflower and kale, the water-insoluble fraction and the monohydric alcohol extract fraction can be ingested as food.

As a form of food, drink [for example,
Powdered beverages, liquid beverages (soft drinks, vegetable juices, etc.)], tablets (tablets), chewable tablets,
Preferred examples include powder, granules, jelly, gummy, candy, candy, fermented foods (eg, yogurt, cheese), biscuits, snacks, breads, cakes, sprinkles, dressings, soups, capsules, and the like.

Among these forms, drinks, tablets, chewable tablets, granules, jellies or biscuits are preferred, and drinks, tablets, chewable tablets and granules are more preferred.

The fraction of the present invention is used as it is for tablets,
It can be prepared into a food form such as a powder according to a usual production method of the food form.

The fraction of the present invention can also be prepared according to a usual production method of the food, using together with other components such as additives usually used in the field of food. For example, at an appropriate time in the process of producing food, any of the fractions of the present invention may be dissolved or dispersed in water, ethanol, or the like, if necessary, added to the food material, and mixed according to a conventional method. And others can be manufactured according to the usual manufacturing method of the food.

Further, the fraction of the present invention can be used for preparing the food by adding it to the food once produced.

As other components, additives usually used in the field of foods can be blended, and examples thereof include starch, lactose, cellulose, dextrin, sugar alcohol,
Excipients such as thickening polysaccharides; sweetening components such as sucrose, maltose, sorbitol, mannitol, and oligosaccharides; nutritional supplementing components such as vitamins and calcium; and fiber components such as apple fiber, corn fiber, and alginic acid.

Alternatively, edible plants other than broccoli, cauliflower and kale may be blended as other components. Other edible plants include soy, oak, barley, celery, lettuce, spinach, komatsuna, parsley, lemon,
Examples include oranges, carrots, radishes, pumpkins, peppers, sweet potatoes and the like.

When the fraction of the present invention is used together with other components to form a food, the mixing ratio is not particularly limited, and can be appropriately set according to the food form and the like. If it is a solid food, the fraction of the present invention may contain about 1 to 99.9% by weight as a dry weight.
The intake of the food containing the fraction of the present invention is not particularly limited, and can be appropriately determined depending on the form of the food, the purpose of the intake, and the like. Usually, it is preferable to ingest the fraction of the present invention in such an amount that the dry weight is about 0.1 to 100 g per adult per day.

The supernatant fraction of broccoli, cauliflower and kale and the fraction extracted with monohydric alcohol have the effect of promoting excretion of bile acids.

The monohydric alcohol fraction of broccoli, cauliflower and kale has an effect of lowering low-density lipoprotein cholesterol (LDL-Chol).

The supernatant fraction and the water-insoluble fraction of broccoli, cauliflower and kale have an action of lowering the neutral fat level.

Further, the monohydric alcohol fraction of broccoli, cauliflower and kale has an action of promoting the growth of bifidobacteria, which are so-called good bacteria, in the intestinal flora (intestinal flora).

Therefore, the food of the present invention containing these fractions has an effect of promoting bile acid excretion, an effect of lowering low-density lipoprotein cholesterol level, an action of lowering neutral fat level, and growth of bifidobacteria. Has at least one kind of action of promoting the action of the body, and these actions can improve the body environment. In order to improve the internal environment of the present invention, internal purification based on these effects (for example,
Blood purification, intestinal environmental improvement) and the like.

If the excretion of bile acids is promoted, cholesterol lowering action and cancer prevention (removal of carcinogenic substances) can be expected.

In addition, the effect of preventing hyperlipidemia and hypercholesterolemia can be expected by lowering the LDL-cholesterol level.

When the neutral fat level is reduced, an effect of preventing hyperlipidemia and hypertriglyceridemia (hyperneutral lipidemia) can be expected. Also, a large amount of triglyceride in the blood can cause obesity, which can lead to adult diseases (lifestyle-related diseases) such as hypertension and arteriosclerosis.
By having the action of lowering the value of neutral fat, a preventive effect on obesity and adult diseases (lifestyle-related diseases) can be expected.

Further, by promoting the growth of bifidobacteria, the intestinal flora is improved, the growth of good intestinal bacteria is promoted, the growth of bad intestinal bacteria is suppressed, the intestinal environment is improved, and the convenience is improved.
Prevents intestinal spoilage, increases fecal weight, improves bowel movements, regulates tummy tone, etc., and is expected to have an intestinal action.In addition, by growing bifidobacteria in the intestine and adjusting the intestinal flora It can be expected to exert effects such as prevention of cancer, prevention of aging, activation of immunity and the like.

That is, the action of promoting the excretion of bile acids, the action of lowering the triglyceride level, the action of lowering the LDL-cholesterol level, and the action of accelerating the proliferation of bifidobacteria increase, thereby purifying the body (purification of blood, Intestinal environment improvement), as described above, the action of promoting bile acid excretion,
An effect based on the action of lowering the neutral fat level, the action of lowering the LDL-cholesterol level, and the action of promoting the growth of bifidobacteria can be expected.

Therefore, the improvement of the body environment of the present invention can be achieved by promoting the action of promoting the excretion of bile acids, the action of lowering the triglyceride level, the action of lowering the LDL-cholesterol level, and the increase of bifidobacteria as described above. Including effects that can be expected from the action of promoting proliferation, especially preventive effect of hyperlipidemia, preventive effect of hypercholesterolemia, preventive effect of hypertriglyceridemia, prevention of obesity, prevention of hypertension, arteriosclerosis Prevention of sickness, improvement of convenience, improvement of intestinal flora, intestinal regulation, activation of immunity, etc. can be expected.

The foods of the present invention containing these fractions can be used as functional foods for improving the body environment, such as health foods, foods for specified health use, dietary supplements, and foods for the sick.

The food of the present invention can also be used as feed (feed) for livestock, pets and the like.

[0053]

The present invention will be described below in more detail with reference to Production Examples, Test Examples and Formulation Examples. Unless otherwise specified, the following percentages represent% by weight (w / w%).

Production Example 1 Preparation of water-soluble and insoluble fractions of broccoli About 13 kg of frozen broccoli puree was thawed at low temperature, immediately diluted with 13 L of water, and centrifuged (12,000 rp).
m, 10 minutes). Centrifugation yielded 20.8 kg of supernatant (428.5 g solids) and 4.4 kg of residue. The residue was further diluted with 20 L of distilled water and centrifuged to obtain 18.6 kg of a supernatant (91.1 g of solids) and 4.4 kg of a residue.

The supernatants were combined and freeze-dried to obtain 560 g of a dry powder (water-soluble fraction). On the other hand, when the residue was freeze-dried, 540 g of a dry powder was obtained (insoluble fraction).

Production Example 2 Preparation of 70% ethanol-extracted fraction of broccoli About 13 kg of frozen broccoli puree was thawed at low temperature and freeze-dried to obtain 1250 g of freeze-dried broccoli. Among them, 1050 g (the remaining 200 g was saved for use in the following test) was 39.4% 70% ethanol.
L, stirred at 40 ° C. for 30 minutes, and centrifuged (12,0
(00 rpm, 10 minutes) to obtain an ethanol-extracted fraction. further,
A 25-fold amount of 70% ethanol was added to the precipitate obtained by centrifugation, and the mixture was stirred at 40 ° C for 30 minutes, and then centrifuged (12,000
rpm, 10 minutes) to obtain an ethanol-extracted fraction.

The ethanol-extracted fractions were combined, concentrated and freeze-dried to obtain 410 g of a dry powder.

Test Example 1 Growth-promoting activity of Bifidobacterium (1) Sample The ethanol-extracted fraction of broccoli obtained in Production Example 2 was used.

(2) Test strain Bifidobacterium longum
ngum JCM1217) was used as a test strain. (3) Test method (3-1) Preparation of a pre-culture solution of the test strain One platinum loop from a colony of the test strain grown on GAM agar medium (Nissui Pharmaceutical) And inoculated in a GAM broth medium for 24 hours at 37 ° C. under anaerobic conditions (H ₂ : 10%, CO ₂ : 5).
%, N ₂ : 85%), and the turbidity was 0.5 (wavelength: 620).
nm) with purified water. (3-2) Test of growth promoting effect A GAM bouillon medium (Nissui Pharmaceutical) was dissolved in purified water as a basic medium and sterilized at 121 ° C for 20 minutes. A test medium was prepared by adding 0.4 g / ml of the ethanol-extracted fraction, which had been sterilized with a sterile filter (0.45 μm), to this basic medium.

The test medium was inoculated with a pre-cultured solution of the test strain prepared in (3-1) in an amount of 1%. After measuring the turbidity of the culture after inoculation, the culture was anaerobically cultured at 37 ° C. for 24 hours, and the turbidity of the obtained culture was measured.

The control was the same except that purified water was added to the basal medium instead of the ethanol-extracted fraction.

From the results of the measurement of the turbidity of the culture, the growth promotion ratio of each sample to the test strain was calculated according to the following equation.

Growth promotion rate (fold) = (T ₂₄ −T ₀ ) / (C ₂₄ −C ₀ ) T ₂₄ : Turbidity of the culture solution containing the ethanol extract fraction after culturing for 24 hours T ₀ : Before culturing C ₂₄ : turbidity of control culture after culturing for 24 hours C ₀ : turbidity of control culture before starting culture.

(4) Test Results The growth promotion rate of the ethanol-extracted fraction was 4.8-fold.

Test Example 2 (1) Sample Water-soluble fraction of broccoli obtained in Production Examples 1 and 2,
A water-insoluble fraction and an ethanol-extracted fraction were used.

(2) Test method Wistar rats (male, 5 weeks old at the time of purchase, weight about 90-110 g,
(Purchased from Japan SLC Co., Ltd.) were raised individually in stainless steel cages. The indoor environment was 23.5 ± 2 ° C. and 55 ± 10 ° C. humidity. During the pre-breeding period (2-3 days), CE-2 manufactured by CLEA Japan was used as a feed and allowed to freely ingest.

As the experimental food, the following feeds were ingested. That is, a high-fat diet (protein source was casein) loaded with 0.5% cholesterol was used as a control diet.
Table 1 and Table 2 show the lyophilized product of each fraction in the control diet.
And the weight was adjusted with sucrose and starch. For comparison, a control meal was prepared by adding a freeze-dried product of broccoli itself (broccoli hole) to the concentration shown in Table 1. Food was freely available during the experiment.

After feeding the experimental food for 10 days, fasting for 24 hours,
The whole blood was collected from the heart under ether anesthesia, and the serum was sampled by an ordinary method.

The serum LDL-cholesterol value was represented by a value obtained by subtracting the HDL-cholesterol value from the total cholesterol value. Thus, the values also include VLDL-cholesterol and IDL-cholesterol. Total cholesterol level was measured by v-cholesterase, manufactured by Nissui
Cholesterol levels were measured by HDL-cholesterase, Nissui Co., Ltd. Triglyceride G-
It was measured by an enzyme method using Test Wako (manufactured by Wako Pure Chemical Industries, Ltd.).

The measurement of acidic steroids (bile acids) was performed as follows. That is, feces for three days after feeding the experimental diet for 10 days were collected, dried for 72 hours (60 ° C.), and pulverized by a mixer. After crushing the feces, acetic acid ethanol (acetic acid: water:
The mixture was extracted with ethyl alcohol = 1: 1: 9), a NaOH solution was added, and neutral lipids were removed with petroleum ether. Then, measurement was performed by an enzymatic method (a bile acid reagent manufactured by Far East Co., Ltd.).

The test was performed in two parts. The results of each test are shown in Tables 1 and 2 below.

[0072]

[Table 1]

[0073]

[Table 2]

(1) In Tables 1 and 2, *, ** and ***
Indicates that there is a significant difference compared to the control group. (*: P <0.05, ** = P <0.01, *** = P <
0.001) (2) In Tables 1 and 2, “bile acid content in feces” and “fecal weight” are the total of feces for three days.

From Table 1, it can be seen that the broccoli (hole) administration group was administered with the 5% water-soluble fraction and the 5% water-insoluble fraction even though the triglyceride level did not change much as compared with the control group. It can be seen that the neutral fat value is significantly reduced in each group.

Further, it can be seen that the amount of bile acid excreted hardly increased in the broccoli-administered group, but the amount of bile acid excreted significantly increased in the group administered with the 5% water-soluble fraction.

As shown in Table 2, compared with the control group, 2.
It can be seen that the LDL-cholesterol level was reduced in the groups administered with the 5% water-soluble fraction and the 2% ethanol-extracted fraction.

Further, in the groups to which the 2.5% water-soluble fraction and the 2% ethanol-extracted fraction were administered, the amount of bile acid excreted was increased in each case.

Hereinafter, examples of the formulation of the food of the present invention will be described. In the following formulation examples, “%” means “% by weight”.

Formulation Example 1 Tablet Lactose 80.0% Gelatin 10.0% Broccoli supernatant fraction freeze-dried product 2.0% Fruit flavor 0.5% Water 7.5% Total 100.0%.

Formulation Example 2 Gum jelly Gum arabic 18.0% Galactooligosaccharide 23.0% Cauliflower ethanol extract fraction concentrate 18.0% Water syrup 23.0% Water 18.0% Total 100.0%.

Formulation Example 3 Jelly Fructose 5.0% Agar 1.0% Broccoli ethanol extract fraction freeze-dried product 4.0% Water 90.0% Total 100.0%.

Formulation Example 4 Juice Broccoli Supernatant Fraction Concentrate 10.0% Concentrated Lemon Juice 0.5% Flavor 0.5% Dye Trace Water 89.0% Total 100.0%.

Formulation Example 5 Granules Lactose 90.0% Talc 0.5% Broccoli water-insoluble fraction freeze-dried product 5.0% Fruit flavor 0.5% Water 4.0% Total 100.0%.

Formulation Example 6 Capsule Kale water-insoluble fraction 50.0% Gelatin 5.0% Glycerin 45.0% Total 100.0%

[0086]

According to the food of the present invention, at least one of an action of promoting excretion of bile acids, an action of lowering LDL cholesterol level, an action of lowering triglyceride level and an action of promoting the growth of bifidobacteria. , The body environment is improved.

 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Akira Takeuchi 2-9-1-4-805 Nasarahara, Takatsuki-shi, Osaka F-term (reference) 4C091 AA01 BB06 CC01 DD01 EE04 FF01 GG01 HH01 JJ03 KK01 LL01 MM03 NN01 PA02 PA05 PB05 QQ01

Claims (4)

[Claims] 1. A supernatant fraction of at least one selected from the group consisting of broccoli, cauliflower and kale.
Either the monohydric alcohol extraction fraction or the water-insoluble fraction 1
A food that improves the body environment containing seeds. 2. An in-vivo environment-improving food comprising at least one supernatant fraction and a monohydric alcohol extract fraction selected from the group consisting of broccoli, cauliflower and kale. 3. An in-vivo environment-improving food containing at least one water-insoluble fraction and a monohydric alcohol-extracted fraction selected from the group consisting of broccoli, cauliflower and kale. 4. An improvement in the body environment, the effect of promoting bile acid excretion,
It is based on at least one kind of action selected from the group consisting of a neutral fat level lowering action, an LDL-cholesterol level lowering action and a bifidobacterium growth promoting action.
Food according to any of the above.