JP2001066290A - Making apparatus for electrophoretic gel plate - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a making apparatus by which an assembly composed of two glass plates and of a spacer of surrounded as a whole closely from its outer circumference so as to be closed airtightly and by which the leak space of a gel solution filled into the gas between the glass plates is made minimum. SOLUTION: In an assembly which is composed of two glass plates 3 and of a spacer 10, a seal gasket in conventional cases is not used, only the spacer 10 is arranged so as to be aligned with end edges of the glass plates, and an effective area as a gel is made maximum. As a result, although a seal is worsened, the assembly is surrounded as a whole closely from its outer circumference so as to be closed airtightly, and the leak space of a gel solution which is filled into the gap between the glass plats is made minimum.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a gel maker used in a vertical slab type electrophoresis apparatus.

[0002]

2. Description of the Related Art In general, a gel maker of this type for use by loading it into an electrophoresis apparatus has a spacer for determining a gap between glass plates sandwiched between two opposing sides of a two-layered equal-sized rectangular flat glass plate. Further, a sealing gasket slightly thicker than the spacer is sandwiched outside the spacer, and the two flat glass plates are pressed from the outside to crush the sealing gasket, so that the gel solution injected into the gap between the glass plates leaks. I was not there.

For this reason, it is necessary to press the surfaces of the stacked glass plates on average, so that the average pressing mechanism is not only complicated, but also the glass plate may be damaged. Further, since the spacer is placed slightly inside the edge of the glass plate, the gel area has to be sacrificed.

[0004]

The present invention proposes a gel maker which eliminates the above disadvantages. In the present invention, the above-mentioned conventional seal gasket is not used, and the spacer is arranged outward until it is aligned with the edge of the glass plate, thereby maximizing the effective area of gel preparation. For this reason, the seal is deteriorated, but instead, the assembly of the two glass plates and the spacer is tightly surrounded and sealed from the outer periphery as a whole, and the leak space of the gel solution filled in the gap between the glass plates is reduced. Minimize.

[0005]

According to the present invention, a spacer is sandwiched between two opposing sides of a two-sided square glass plate with the edges aligned with the two glass plates. The assembly of the two glass plates and the spacers is dropped into the box with substantially the same edge and thickness as the glass plate, and the frame of the box and the glass plate are flush with each other. A series of gaskets are placed over the joint surface, which is pressed, and pressed with a lid to prevent the gel solution leaking through the spacer from leaking out of the box.

This pressing does not actively prevent the gel solution from leaking through the spacer as in the prior art, so that there is no risk of damaging the glass plate. The gel solution leaking to the three-dimensional back surface is small, and even if it leaks, the glass surface only needs to be wiped with a cloth. If necessary, the edge may be wiped with a cloth.
In addition, the material of the box is a material in which the gel solution hardly causes a polymerization reaction,
For example, it is preferably made of acrylic or silicon rubber.

[0007]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS A preferred embodiment of the present invention will be described with reference to the drawings. Acrylic plate 1 and frame 1 in the left half of FIG.
6, acrylic plate 2 and frame 17 in the right half
The lid of this box consisting of: The box body and the lid are crimped by a latch mechanism that hooks and tightens the lever 4 on the projection 6 as shown by the arrow.

First, an assembly of two glass plates 9 and 8 and a pair of spacers 10 is fitted exactly in the box.
The surface of the glass plate that appeared on the surface is joined to the upper surface of the frame 16 on the same plane, the surface of the glass plate that has entered the inside of the box is in close contact with the bottom of the box, and the end surface (edge) of the assembly is on the side of the frame 16. Close contact and no space. Note that one side of this quadrilateral assembly, that is, the upper side in FIG. 1 is a gel solution inlet, so that this side is naturally open.

Next, as shown by an arrow, the box is closed, and a series of U-shaped gaskets 11 are provided on the inner surface of the lid shown in the right half of FIG. The glass solution and the upper surface of the frame 16 joined on the same plane are in contact with each other across the joint line, and the gel solution leaking from the edge of the assembly is confined in the space between the edge of the assembly and the frame 16. This pattern is shown in FIG.

The lid rotates the levers 4 provided on both sides of the acrylic plate 1 and cuts the notches 5 into the acrylic plate 2.
This is achieved by engaging the strip-shaped gasket 11 against the projections 6 on both side surfaces of the belt.

Finally, the gel solution is injected into the gap between the glass plates from the gel solution injection port on the upper side of FIG. 1 and polymerized. Since the leaked solution is in contact with the acrylic, it hardly polymerizes and wiping is sufficient.

[0012]

According to the present invention, since no strong pressure is applied to the assembly, there is no glass breakage, and substantially no operation force is required.

[Brief description of the drawings]

FIG. 1 is a perspective view showing an embodiment of a gel maker according to the present invention.

FIG. 2 is an explanatory diagram of the operation of FIG.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 ... Acrylic plate 2 ... Acrylic plate 3 ... Glass plate assembly 4 ... Lever 8, 9 ... Glass plate 10 ... Spacer 11 ... Gasket

Claims (3)

[Claims] 1. An electrophoresis gel maker for stacking two flat equilateral quadrangular glass plates with a spacer for setting a gap therebetween and forming a gel in a gap between the two glass plates. The two glass plates are sandwiched so that the edges are aligned in the gap between the two opposing sides of the rectangular glass plate, and then this assembly is dropped into a box in which both its thickness and its peripheral edge fit tightly. The gasket is sealed by a lid in which a series of gaskets are arranged in a U-shape so as to straddle the frame and the glass plate surface of the box and make contact with the opposite two sides and the bottom side. Electrophoresis gel maker. 2. The electrophoresis gel maker according to claim 1, wherein said box is made of acrylic. 3. The electrophoretic gel maker according to claim 1, wherein said box is made of silicone rubber.