JP2001040513A - Care clothing having feature of supporting microorganism belonging to genus branchybacterium - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a care clothing excellent in antibacterial property against Staphylococcus aureus, having high safety to the human body and deodorizing effect, and useful for clothing for sleeping, gloves, hosiery, a pair of Tabi (Japanese socks), underwear, and the like, by making the clothing support microorganisms belonging to Brachybacterium. SOLUTION: Microoganisms belonging to Brachybacterium such as Brachybacterium strain AAA-a (FERM P-16,951) are supported in the number of preferably 10 to 10,000,000 /cm3. The Brachybacterium preferably is a new microorganism having the following mycological properties: having a form of polymorphismic bacilli; gram staining is positive; spore is negative; motility is negative; attitude for oxygen is aerobic; oxidase is negative; catalase is positive; color tone of the colony is yellowish; antibacterial property is negative; bacillary-globular cycle is positive; elongation of surface cells of the colony is negative; cell wall diamino acid is meso-diaminopimelic acid; glycolyl test is negative (actyl type); or the like.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to clothing for nursing care carrying microorganisms belonging to the genus Brachybacterium.

[0002]

BACKGROUND OF THE INVENTION As bacteria belonging to the genus Brachybacterium, the following are presently known. Brachybacterium conglomeratum Brachybacterium faecium Brachybacterium nesterenkovii Brachybacterium paraconglomeratum Brachybacterium rhamnosum However, at present, these brachybacteriums have not been studied for their industrial utility.

[0003]

The inventors of the present application have conducted intensive studies on Brachybacterium, and have found that Brachybacterium has antibacterial activity against Staphylococcus aureus. An object of the present invention is based on this finding, and it is an object of the present invention to provide a nursing garment carrying the above Brachybacterium.

[0004]

The above objects can be attained by the following constitutions (1) to (6). (1) Clothing for nursing care carrying microorganisms belonging to the genus Brachybacterium. (2) The clothing for nursing care according to claim 1, wherein the Brachybacterium is a novel microorganism belonging to the genus Brachybacterium having the following mycological properties. 1. Form: polymorphic bacilli 2. Gram stainability: +3. Spores: -4. Motility: -5. 5. Attitude to oxygen: aerobic Oxidase: -7. Catalase: +8. Village color tone: yellow 9. Acid resistance: -10. Rod-spherical cycle: +11. 11. Extension of cells around the colony: -12. 12. Cell wall diamino acids: meso-diaminopimelic acid Glycolyl test:-(acetyl type) 14. Alahino caractan polymer on the cell wall: -15. Mycolic acid: -16. Quinone type: MK-7, -8 17. GC content of intracellular DNA: 72 mol% Nitrate reduction: +19. Denitrification reaction: +20. Methyl red test: -21. V-P reaction:-22. Indole production:-23. Production of hydrogen sulfide TSI agar:-Lead acetate agar:-24. Hydrolysis of starch: +25. Utilization of citrate Koser's medium:-Christensen's medium:-26. Use of inorganic nitrogen source Nitrate:-Ammonium salt: Weak 27. Formation of pigment: + (yellow) 28. Urease:-29. Oxidase: -30. Range of growth pH: 6.0 to 10.0 Temperature: 9 to 42 ° C 31. Generation of acid D-arabinose:-L-arabinose: + D-xylose: + D-glucose: + D-mannose: + D-fructose: + D-galactose: + maltose: + sucrose: + lactose: + trehalose: + D-sorbitol: -D-mannitol: + inositol: -glycerin: + starch: + meletitose: -D-ribose: -32. Generation of gas L-arabinose: -D-xylose: -D-glucose: -D-mannose: -D-fructose: -D-galactose: -maltose: -sucrose: -lactose: -trehalose: -D-sorbitol: -D-mannitol:-inositol:-glycerin:-starch:-(3) The nursing care garment according to the above (1) or (2), which is a sleeping garment. (4) The nursing clothing according to the above (1) or (2), which is a glove. (5) The nursing garment according to the above (1) or (2), which is a sock or a tabi. (6) The nursing garment according to the above (1) or (2), which is underwear. Testing and classification of the mycological properties of the novel Brachybacterium described above were performed according to the following literature.

[0005] International Journal of Systematic Bacteriology (International J
ournal Systematic Bacteriology), Volume 38, Volume 4
Pages 5 to 48 (1988) International Journal Systect Bacteriology
ematic Bacteriology), Vol. 42, pp. 74-78 (1992) International Journal Syst.
ematic Bacteriology, Vol. 46, pp. 81-87 (1996) International Journal Syst.
ematic Bacteriology), Vol. 45, Nos. 160-168
Page (1995)

As a result, the microorganism was identified as a Brachybacterium because it was a spore-free gram-positive bacillus exhibiting polymorphism. Therefore, it was confirmed that the strain was a new strain belonging to Brachybacterium. The microorganism is Brachybacterium.
ium) strain AAA-a (deposit number: FERM P-1695)
1) Deposited in the Microbial Depositary Center of the National Institute of Bioscience and Human Technology, National Institute of Advanced Industrial Science and Technology.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The care clothing of the present invention carries Brachybacterium. It is preferable to use the following novel microorganisms as Brachybacterium. Less than,
The mycological properties of the novel microorganism belonging to Brachybacterium will be described.

[0008] 1. 1. Form: polymorphic bacilli (see FIGS. 1 and 2) Gram stainability: +3. Spores: -4. Motility: -5. 5. Attitude to oxygen: aerobic Oxidase: -7. Catalase: +8. Village color tone: yellow 9. Acid resistance: -10. Rod-coccus cycle
: + (See FIGS. 1 and 2) 11. Extension of cells around the colony: -12. 12. Cell wall diamino acids: meso-diaminopimelic acid (estimated from acid hydrolyzate of whole cells) Glycolyl test:-(acetyl type) 14. Alahino-galactan polymer in cell wall:-(estimated from acid hydrolyzate of whole cells) Mycolic acid: -16. Quinone type: MK-7, -8 GC content of intracellular DNA: 72 mol% (HPL
C method) 18. Nitrate reduction: +19. Denitrification reaction: +20. Methyl red test: -21. V-P reaction:-22. Indole production:-23. Production of hydrogen sulfide TSI agar:-Lead acetate agar:-(Tested by hanging lead acetate test paper without adding lead acetate) 24. Hydrolysis of starch: +25. Utilization of citrate Koser's medium:-Christensen's medium:-26. Use of inorganic nitrogen source Nitrate:-Ammonium salt: Weak 27. Formation of pigment: + (yellow) 28. Urease:-29. Oxidase: -30. Growth range (tested with broth broth.
The following pH was 0.5, and the temperature was tested in steps of 1 ° C.) PH: 6.0-10.0 Temperature: 9-42 ° C Optimum growth conditions pH: 7.5-9.0 Temperature: 18-27 ° C 31. Generation of acid D-arabinose:-L-arabinose: + D-xylose: + D-glucose: + D-mannose: + D-fructose: + D-galactose: + maltose: + sucrose: + lactose: + trehalose: + D-sorbitol: -D-mannitol: + inositol: -glycerin: + starch: + meletitose: -D-ribose: -32. Generation of gas L-arabinose: -D-xylose: -D-glucose: -D-mannose: -D-fructose: -D-galactose: -maltose: -sucrose: -lactose: -trehalose: -D-sorbitol: -D-mannitol:-inositol:-glycerin:-starch:-

[0009] The novel microorganisms described above grow well on any nutrient medium. The same applies to other Brachybacterium.

[0010] The culture preferably grows well at about 5 to 30 ° C, most preferably about 18 to 27 ° C.

The morphological properties of the novel microorganism are as follows. (1) Cell shape and size: When cultured using broth agar medium: 1.2 to 1.4 x 1.5 to 30 ° C for 6 hours
It is a 1.9 micron polymorphic short rod.・ 1.2 to 1.4 × 1.2 to culture at 30 ° C. for 24 hours
It shows 1.5 micron cocci. When cultured using broth liquid medium: 1.0-1.2 × 1.3- at 30 ° C. for 24 hours
Shows 1.4 micron cocciform. (2) Cell polymorphism: polymorphism and rod-spherical cycle are observed (see FIGS. 1 and 2). (3) No motility is observed. (4) No spore formation is observed.

The culture characteristics of the above novel microorganisms in each medium are as follows. (1) When cultured using a broth agar plate medium:-The colonies are smooth and gloss is observed at the entire edge. -The generation of yellowish pigments is observed. (2) In the case of culturing using a broth liquid medium:-Growth and precipitation are observed in the entire medium. -No surface film is formed. (3) When cultured using gravy gelatin puncture:-Growth is observed on the upper part of the medium, but no liquefaction is observed. (4) In the case of culturing using litmus milk: ・ Growth is observed in the whole medium, but no coagulation or liquefaction is observed.・ Slight generation of acid is observed. Brachybacterium, including the novel microorganisms described above,
It is dispersed in water or a liquid medium and is carried on cloth or paper clothing. As the cloth clothing, a woven fabric or a nonwoven fabric can be used. The material is made of natural fiber such as cotton or hemp, or synthetic fiber such as nylon, polypropylene, or polyester, and is formed in the form of woven or non-woven fabric, or paper (hereinafter sometimes referred to as carrier). As a substrate. Brachybacterium is carried on the carrier made of the cloth or the like as described in detail below. The brachybacterium-carrying part may be a part of the cloth or the like, or may have a structure in which a brachybacterium-carrying cloth or paper ribbon or the like is sewn or attached to clothing. Is also good.

As a material of a part of the cloth constituting the garment, it is preferable to use a fiber in which particles of oxide ceramics having far-infrared radiation characteristics are mixed, preferably inside. The far-infrared radiation oxide ceramics used herein include those obtained by adding an M metal oxide such as titanium oxide, zirconium oxide, and tin oxide to clay and sintering them. Specifically, ZrO ₂ —SiO ₂ Ceramics A
l ₂ O ₃ -SiO ₂ based ceramics _· TiO ₂ -Cr 2
O ₃ based ceramics _{· Al 2 O 3 - (Si} , Ti) O 2
Ceramics (Al, Fe, B, Cr) ₂ O ₃ -S
iO _2- (alkali metal, alkaline earth metal) oxide-based ceramics.

Here, when the oxide ceramic is heated to 35.5 to 36.5 ° C. which is substantially equal to the body temperature, the oxide ceramic emits an electromagnetic wave having a wavelength peak of 8 to 14 μm, which coincides with the infrared absorption wavelength of the human body. Those having far-infrared radiation characteristics are used.

In order to support the microorganisms on the carrier as described above, a dispersion of the microorganisms prepared by dispersing the microorganisms in water or a liquid medium is sprayed on the carrier. Good.

The amount of microorganisms carried on the carrier is as follows:
Although it varies depending on the conditions, preferably 5 to 20
100 million pieces / cm³, More preferably 10 to 10 billion /
cm ³It is.

[0017] The brachybacterium-carrying garment of the present invention comprises:
It is effective for antibacterial and eradication of Staphylococcus aureus, and can be expected to have the effect of preventing bedsores and the like in long-term care recipients. In addition, a deodorizing effect was confirmed.

The safety of the novel microorganism was confirmed by an oral administration test using mice. From the above effects, the above-mentioned brachybacterium-carrying clothing can be used as clothing for nursing care.

[0019]

Embodiment 1 Next, an embodiment of the present invention will be described. First, a medium having the following composition was prepared.

Medium Peptone 5 g Meat extract 5 g Water 500 ml Brachybacterium strain AAA-a was inoculated into a medium having a composition of pH 6.8 ± 0.2 or more and cultured at 30 ° C. with shaking. The medium that has reached the logarithmic phase is diluted with sterile physiological saline, and the viable cell count is about 1 × 1
0 8 ^/ and ml to be prepared, was inoculated by sprinkling the preparation directly Staphylococcus aureus, characteristics halos were observed. When the same experiment was performed for the other Brachybacterium described above, a halo property was observed for Staphylococcus aureus, although the function was inferior to that of the novel strain.

Example 2 Brachybacterium strain AAA-a was inoculated into a heart infusion and bouillon medium and cultured at 30 ° C. with shaking. The medium that has reached the logarithmic phase is diluted with sterile physiological saline to prepare a viable cell count of about 1 × 10 ⁸ / ml. Cloth the prepared water so that the number of viable bacteria is about 1 × 10 ⁴ / cm ² .
It is applied to a carrier such as paper and dried. A yukata was created using the materials thus made. When this was put on a bedridden patient, bedsore did not occur even after 6 months. Outside of this yukata, patients in the same environment had bedsores in one month for early persons and three months for late persons. No special equipment for preventing bedsore was used in any case.

The bacteria grow to 3 × 10 ⁷ / cm ² after 6 hours due to nutrients such as sweat. Staphylococcus aureus is removed by the characteristics of the halo of the grown Brachybacterium. It is thought that pneumonia, sepsis, osteomyelitis, staphylococcal enteritis, staphylococcal burn-like syndrome, and toxic shock syndrome, which are caused by staphylococci, can be prevented.

[Brief description of the drawings]

FIG. 1 is a photograph substituted for a drawing, and is a micrograph showing a preferred form of a novel microorganism used in the clothing of the present invention.

FIG. 2 is a photograph substituted for a drawing, and is a micrograph showing a preferred form of a novel microorganism used in the clothing of the present invention.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) // A61L 2/00 A61L 2/00 F term (Reference) 3B011 AA01 AA07 AB08 AC19 3B018 AC09 3B033 AB12 AC05 AC06 4B065 AA01X AC12 AC20 BC41 BC42 CA34 CA44 4C058 AA05 AA08 BB10 JJ04

Claims (6)

[Claims] 1. A nursing garment carrying a microorganism belonging to the genus Brachybacterium. 2. The nursing garment according to claim 1, wherein the Brachybacterium is a novel microorganism belonging to the genus Brachybacterium having the following mycological properties. 1. Form: polymorphic bacilli 2. Gram stainability: +3. Spores: -4. Motility: -5. 5. Attitude to oxygen: aerobic Oxidase: -7. Catalase: +8. Village color tone: yellow 9. Acid resistance: -10. Rod-spherical cycle: +11. 11. Extension of cells around the colony: -12. 12. Cell wall diamino acids: meso-diaminopimelic acid Glycolyl test:-(acetyl type) 14. Alahino caractan polymer on the cell wall: -15. Mycolic acid: -16. Quinone type: MK-7, -8 17. GC content of intracellular DNA: 72 mol% Nitrate reduction: +19. Denitrification reaction: +20. Methyl red test: -21. V-P reaction:-22. Indole production:-23. Production of hydrogen sulfide TSI agar:-Lead acetate agar:-24. Hydrolysis of starch: +25. Utilization of citrate Koser's medium:-Christensen's medium:-26. Use of inorganic nitrogen source Nitrate:-Ammonium salt: Weak 27. Formation of pigment: + (yellow) 28. Urease:-29. Oxidase: -30. Range of growth pH: 6.0 to 10.0 Temperature: 9 to 42 ° C 31. Generation of acid D-arabinose:-L-arabinose: + D-xylose: + D-glucose: + D-mannose: + D-fructose: + D-galactose: + maltose: + sucrose: + lactose: + trehalose: + D-sorbitol: -D-mannitol: + inositol: -glycerin: + starch: + meletitose: -D-ribose: -32. Generation of gas L-arabinose: -D-xylose: -D-glucose: -D-mannose: -D-fructose: -D-galactose: -maltose: -sucrose: -lactose: -trehalose: -D-sorbitol: -D-mannitol:-inositol:-glycerin:-starch:- 3. The clothing for nursing care according to claim 1, which is clothing for sleeping. 4. The clothing for nursing care according to claim 1, which is a glove. 5. The nursing garment according to claim 1, which is a sock or a tabi. 6. The clothing for nursing care according to claim 1, which is underwear.