JP4918519B2 - Nursing care clothes carrying microorganisms belonging to the genus Brachybacterium - Google Patents

Description

  [Technical Field] The present invention relates to a nursing garment carrying a microorganism belonging to the genus Brachybacterium.

At present, the following bacteria are known as bacteria belonging to the genus Brachybacterium.

Brachybacterium conglomeratum

Brachybacterium faecium

Brachybacterium nesterenkovii

Brachybacterium paraconglomeratum

Brachybacterium rhamnosum
However, there is currently no research on the industrial applicability of these Brachybacterium.

Thus, the inventors of the present application conducted extensive research on Brachybacterium and found that Brachybacterium has antibacterial properties against Staphylococcus aureus.

The subject of this invention is based on this knowledge and is providing the clothing for nursing care which carry | supported the said Brachybacterium.

The said subject can be achieved by following structure (1)-(5).
(1) Nursing care clothing carrying Brachybacterium strain AAA-a (deposit number: FERM BP- 6848) having antibacterial activity against Staphylococcus aureus.
(2) The nursing clothing described in (1) above, which is a sleeping clothing.
(3) The clothing for nursing care according to (1) above, which is a glove.
(4) The nursing clothes according to (1), which are socks or tabi.
(5) The clothing for nursing care according to (1) above, which is underwear.

1) International Journal Systematic Bacteriology, Vol. 38, pages 45-48 (1988)
2) International Journal Systematic Bacteriology, Vol. 42, pp. 74-78 (1992)
3) International Journal Systematic Bacteriology, 46, 81-87 (1996)
4) International Journal Systematic Bacteriology, Vol. 45, pages 160-168 (1995)

As a result, the microorganism was identified as Brachybacterium because it is a non-spore-free Gram-positive gonococci exhibiting polymorphism. It was confirmed that it was a new bacterium belonging to Brachybacterium. The microorganism is internationally deposited at the Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology as Brachybacterium strain AAA-a ( Accession Number: FERM BP-6848 ).

The nursing clothes of the present invention carry Brachybacterium. As Brachybacterium, it is preferable to use the following novel microorganisms.
Hereinafter, the mycological properties of the novel microorganism belonging to the above Brachybacterium will be described.

1. Form: Polymorphic Neisseria gonorrhoeae (see Fig. 1 and Fig. 2)
2. Gram staining: +
3. Spore:-
4). Motility:-
5. 5. Attitude toward oxygen: aerobic Oxidase:-
7). Catalase: +
8). 8. Color of village: Yellowish Antiacid:-
10. Rod-coccus cycle: + (see Figures 1 and 2)
11. Elongation of surrounding cells in the village:-
12 Cell wall diamino acids: Meso-diaminopimelic acid (whole cell acid
Estimated from hydrolyzate)
13. Glycolyl test:-(acetyl type)
14 Cell wall arabino galactan polymer:-(Whole cell acid hydrolyzate
Estimated)
15. Mycolic acid:-
16. Quinone series: MK-7, -8
17. GC content of intracellular DNA: 72 mol%
(By HPLC method)
18. Reduction of nitrate: +
19. Denitrification reaction: +
20. Methyl red test:-
21. V-P reaction:-
22. Generation of indole:-
23. Production of hydrogen sulfide
TSI agar:-
Lead acetate agar:-(Lead acetate without adding lead acetate
Test by hanging a test paper)
24. Starch hydrolysis: +
25. Use of citrate
Koser's medium:-
Christensen's medium:-
26. Use of inorganic nitrogen source
Nitrate:-
Ammonium salt: Weak 27 Pigment production: + (yellowish)
28. Urease ： −
29. Oxidase:-
30. Range of growth (tested using broth broth.
The test was conducted in 1 ° C increments)
pH: 6.0 to 10.0
Temperature: 9-42 ° C
Optimal growth conditions
pH: 7.5-9.0
Temperature: 18-27 ° C
31. Acid generation
D-arabinose:-
L-arabinose: +
D-xylose: +
D-glucose: +
D-Mannose: +
D-fructose: +
D-galactose: +
Maltose: +
Sucrose: +
Lactose: +
Trehalose: +
D-sorbitol:-
D-mannitol: +
Inositol:-
Glycerin: +
Starch: +
Meletetose:-
D-ribose:-
32. Gas generation
L-arabinose:-
D-xylose:-
D-glucose:-
D-Mannose:-
D-fructose:-
D-galactose:-
Maltose:-
Sucrose:-
Lactose:-
Trehalose:-
D-sorbitol:-
D-mannitol:-
Inositol:-
Glycerin:-
Starch:-

  The novel microorganisms grow well in any medium that is a general nutrient medium. The same is true for other Brachybacterium.

  The culture preferably grows well at about 5 to 30 ° C., most preferably about 18 to 27 ° C.

The morphological properties of the above novel microorganism are as follows.
(1) Cell shape and size:
1) When cultured using a broth agar medium-It is a polymorphic short rod of 1.2-1.4 × 1.5-1.9 microns when cultured at 30 ° C. for 6 hours.
-It shows 1.2-1.4x1.2-1.5 micron-like cocci in the culture at 30 ° C for 24 hours.
2) When cultured using a broth liquid medium • Shows 1.0-1.2 × 1.3-1.4 micron cocci-like culture at 30 ° C. for 24 hours.
(2) Cell polymorphism:
A polymorphism and a saddle-spherical cycle are observed (see FIGS. 1 and 2).
(3) No motility is observed.
(4) Spore formation is not observed.

The culture properties of the above-mentioned novel microorganisms in each medium are as follows.
(1) When cultured using a broth agar plate medium:
・ The village is smooth and glossy at all edges.
・ Yellow colony pigment formation is observed.
(2) When cultured using a broth liquid medium:
-Growth and precipitation in the whole medium are observed.
・ Surface film formation is not observed.
(3) When cultured using gravy gelatin puncture:
・ Growth is observed at the top of the medium, but no liquefaction is observed.
(4) When cultured using litmus milk:
・ Growth is observed throughout the medium, but coagulation and liquefaction are not observed.
-Slight acid formation is observed.
Brachybacterium including the above-mentioned novel microorganisms are dispersed in water or a liquid medium, and are carried on cloth or paper clothing. As the cloth apparel, a woven fabric or a non-woven fabric can be used. Materials include natural fibers such as cotton and linen, and chemical fibers such as nylon, polypropylene, and polyester, and fabrics formed in the form of woven fabrics and nonwoven fabrics, or paper (hereinafter sometimes referred to as carriers). Is the substrate. Brachybacterium is supported on the carrier made of the cloth or the like as described in detail below. The brachybacterium carrying part may be a part of the above-mentioned cloth or the like, or has a structure in which a brachibacteria carrying cloth or a paper ribbon or the like is sewed or attached to clothing. Also good.

  It is preferable to use a fiber in which particles of oxide ceramics having far-infrared radiation characteristics are desirably mixed inside as a material of a part of the cloth constituting the clothing. The far-infrared radioactive oxide ceramics used here include those obtained by adding M metal oxides such as titanium oxide, zirconium oxide and tin oxide to clay and sintering them. Specifically, ZrO2-SiO2 ceramics・ Al 2 O 3 —SiO 2 ceramics • TiO 2 —Cr 2 O 3 ceramics • Al 2 O 3 — (Si, Ti) O 2 ceramics • (Al, Fe, B, Cr) 2 O 3 —SiO 2 (alkali metal, alkaline earth metal) oxide ceramics Is mentioned.

  Here, when the oxide ceramics is heated to 35.5 to 36.5 ° C., which is substantially equal to the body temperature, the far-infrared radiation that emits electromagnetic waves having a wavelength peak of 8 to 14 μm, which matches the infrared absorption wavelength of the human body. Those with radiation characteristics are used.

  In order to support the microorganism group on the carrier as described above, a microorganism group dispersion liquid prepared by dispersing the microorganism group in water or a liquid medium is sprayed on the carrier.

  The amount of the microorganism group supported on the carrier is preferably 5 to 20 billion / cm 3, more preferably 10 to 10 billion / cm 3, although it varies depending on the conditions for loading.

  The brachybacterium-supporting clothing of the present invention is effective for antibacterial and sterilization of Staphylococcus aureus, and can be expected to prevent bed slipping and the like of long-term care recipients. Moreover, the deodorizing effect was confirmed.

  The safety of the above-mentioned novel microorganisms was confirmed by an oral administration test using mice. From the above effects, the Brachybacterium-carrying garment can be used as a nursing garment.

Example 1
Next, examples of the present invention will be described.
First, a medium having the following composition was prepared.

Medium 5g peptone
5g meat extract
Water 500m1
Medium pH 6.8 ± 0.2

The medium having the above composition was inoculated with Brachybacterium strain AAA-a and cultured with shaking at 30 ° C. The medium that reached the logarithmic phase was diluted with sterilized physiological saline and prepared so that the number of viable bacteria was about 1 × 10 8 / ml. When this preparation was directly inoculated by sprinkling on staphylococci, The characteristics were recognized. When the same experiment was conducted for the other Brachybacterium, a halo characteristic was observed against Staphylococcus aureus, although the function was inferior to that of the new strain.

Example 2
Brachybacterium strain AAA-a was inoculated into heart infusion and broth medium, and cultured with shaking at 30 ° C.
The medium that has reached the logarithmic phase is diluted with sterilized physiological saline to prepare a viable cell count of about 1 × 10 8 / ml. The prepared water is applied to a carrier such as cloth or paper so that the viable count is about 1 × 10 4 / cm 2 and dried. Yukata was made using the material made in that way. When this was worn by a bedridden patient, no bed slip occurred even after 6 months.
A patient with the same environment outside of this yukata had a bed slip in one month for the early person and three months for the late person. In all cases, no special equipment for preventing bedsores was used.

  The fungus grows to 3 × 10 7 / cm 2 after 6 hours due to nutrients such as sweat. Staphylococcus aureus is removed by the halo characteristics of the grown Brachybacterium. It may be possible to prevent pneumonia, sepsis, osteomyelitis, staphylococcal enteritis, staphylococcal burn-like syndrome, and toxic shock syndrome caused by staphylococci.

It is a drawing-substituting photograph, and is a micrograph showing the preferred form of a novel microorganism used in the apparel of the present invention. It is a drawing-substituting photograph, and is a micrograph showing the preferred form of a novel microorganism used in the clothing of the present invention.

Claims (5)

Nursing care clothing carrying Brachybacterium strain AAA-a (deposit number: FERM BP- 6848) with antibacterial properties against Staphylococcus aureus. The nursing clothing according to claim 1, which is a sleeping clothing. The clothing for nursing care according to claim 1, which is a glove. The clothing for nursing care according to claim 1, which is a sock or a tabi. The nursing clothing according to claim 1, which is an underwear.

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