JP2000327511A - Plant growth regulator - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject plant growth regulator that can effectively promote rooting and root elongation of a plant and can be readily and inexpensively prepared by adding a liquid culture medium including a carbon source, an organic nitrogen source and inorganic salts that are suitable for specific culture thereto. SOLUTION: The objective plant growth regulator is prepared by admixing a liquid culture medium containing a carbon source, an organic nitrogen source and inorganic salts, suitable for culturing Bacillus thuringiensis thereto. This liquid medium may be any suitable for culturing an arbitrary TB strain that produces an insecticidal toxic protein. Potato starch is preferably used as the carbon source; the organic nitrogen source is preferably swine meat powder. As inorganic salts, may be cited a sodium source, for example, sodium carbonate and inorganic nitrogen source, for example, triammonium phosphate. The content of the liquid medium is preferably <= about 10 wt.% of the carbon source; about <=10 wt.% of an organic nitrogen source and about <=1% of inorganic salts.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a plant growth regulator,
In particular, BT (Bacillus thuringien)
The present invention relates to a plant growth regulator using a liquid medium for culturing sis).

[0002]

BT is a Bacillus subtilis that grows in soil and is called δ-endotoxin during its sporulation stage.
It is known to produce toxin proteins with insecticidal activity. A preparation containing BT microbial cells (BT agent) has been put to practical use as a biopesticide with high environmental safety (for example, Shionogi Pharmaceutical's "Vasilex (registered trademark)").

On the other hand, JP-A-6-172116 discloses that
A plant growth material containing BT bacteria is described. This plant growth material is said to be effective in preventing continuous cropping failure of vegetables and growing trees and lawns. The effect is believed to be due to the protein produced by BT killing soil nematodes.

[0004] However, a liquid medium suitable for BT culture but not containing BT, and a culture supernatant obtained by removing the cell-containing layer after BT culture have a plant growth regulating effect. No examples are known.

[0005]

SUMMARY OF THE INVENTION The present invention aims to provide a novel plant growth regulator. In particular, an object of the present invention is to provide a relatively inexpensive and easily prepared plant growth regulator that can effectively promote rooting and elongation of a plant.

[0006]

Means for Solving the Problems The present inventors have found that the liquid medium for BT culture and the supernatant of the BT culture have a plant growth promoting action such as an increase in the number of roots and root length of the plant. Was. Furthermore, it has been found that when indolebutyric acid is added to these liquid preparations, plant growth is more remarkably activated. The present invention has been completed based on these findings.

The present invention relates to a BT (Bacillus th
a plant growth regulator comprising a liquid medium containing a carbon source, an organic nitrogen source and an inorganic salt, suitable for the cultivation of C. uringiensis): The organic nitrogen source may comprise pork flour. Also, the carbon source may include potato starch, and the organic nitrogen source may include pork powder and corn steep liquor. The plant growth regulator of the present invention may further contain an antifoaming agent.

[0008] The above liquid medium is prepared by the following steps: (a) preparing a preculture medium containing a part of each of the above-mentioned carbon source, organic nitrogen source and inorganic salt, and adjusting the pH to about 6.0-8. (B) adjusting the preculture medium obtained in the step (a) to room temperature at about 10
(C) preparing a main culture medium containing the above-mentioned carbon source, organic nitrogen source and the remainder of the inorganic salt;
Adjusting the H to be in the range of about 6.0 to 8.0 and then heat sterilizing; (d) the preculture medium obtained in the step (b), and the main culture medium obtained in the step (c); Mix
Stirring the mixed medium obtained at room temperature for about 30 to 60 hours; and (e) degassing the mixed medium obtained in step (d), and then adjusting the pH to about 2.0 to 4.0. Adjusting step.

[0009] The liquid medium may also be a culture supernatant of BT. This culture upper layer solution is prepared by the following steps: (a) preparing a preculture medium containing a part of each of the above-mentioned carbon source, organic nitrogen source and inorganic salt, and adjusting the pH to a range of about 6.0 to 8.0. (B ') adding BT to the preculture medium obtained in step (a),
Stirring at room temperature for about 10 to 30 hours; (c) preparing a main culture medium containing the above-mentioned carbon source, organic nitrogen source and the remainder of the inorganic salt, and adjusting the pH to about 6.0 to 8.0. (C) mixing the preculture medium obtained in step (b ′) with the main culture medium obtained in step (c), and heating the mixed medium at room temperature. About 30-6
(E) degassing the mixed medium obtained in step (d) and then adjusting the pH to a range of about 2.0 to 4.0; and (f) step (e). Removing the culture substratum containing the sediment generated by the method described above.

[0010] The plant growth regulator of the present invention may further contain indolebutyric acid in addition to the above liquid medium.

The plant growth regulator of the present invention contains the above liquid medium or a mixture of the above liquid medium and indolebutyric acid in an amount effective to promote rooting and / or root elongation of plants. Can be Plants of interest are plants belonging to the family Coleoptera, Brassicaceae, Rosaceae, Asteraceae, or Poaceae, especially rose (Rosa polyantha).
Can be

[0012]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail.

(Composition of Plant Growth Regulator) The plant growth regulator of the present invention comprises BT (Bacillus thuring).
liquid medium containing a carbon source, an organic nitrogen source, and an inorganic salt, which is suitable for cultivation of C.iensis). This liquid medium contains any B that produces toxin proteins that exhibit insecticidal activity.
Any material suitable for culturing the T strain may be used.

As a carbon source in the liquid medium, potato starch is typically used, but is not limited thereto. For example, one or more other starches such as corn starch and other carbon sources may be used in combination with potato starch or alone. The carbon source is typically not more than about 10% by weight, based on the total weight of the liquid medium,
Preferably, it can be contained at a ratio of about 2 to 10% by weight.

As the organic nitrogen source in the liquid medium, pork powder or a mixture of pork powder and corn steep liquor is typically used, but is not limited thereto. For example, soybean meal, casein, yeast extract, one or more other organic nitrogen sources such as meat extract other than pork, pork powder, or in combination with a mixture of pork powder and corn steep liquor, or used alone Is also good. The organic nitrogen source is typically about 10% by total weight of the liquid medium.
% By weight, preferably about 2 to 10% by weight.

It is particularly preferred that the plant growth regulator of the present invention contains pork powder as a source of organic nitrogen in a liquid medium. The term “pork powder” as used herein broadly encompasses any pork-derived powdery material, such as pork dry extract, pork extract, and enzymatically decomposed pork. Any component present in the pork powder, or the product of this component interacting or chemically reacting with other components in the liquid medium,
It is possible that they have a plant growth regulating effect. However, this is not intended to limit the invention to a particular mode of action. The pork powder is typically about 50% by weight or more, preferably about 60 to 90% by weight, based on the total weight of the organic nitrogen source.
At a rate of

As the inorganic salt in the liquid medium, known salts suitable for culturing a BT strain can be used. Usually, a sodium source such as sodium carbonate and an inorganic nitrogen source such as triammonium phosphate are added. Further, one or more other inorganic salts such as ammonium sulfate may be added. The inorganic salt may be contained at a ratio of typically about 1% by weight or less, preferably about 0.05 to 1% by weight, based on the total weight of the liquid medium.

The above liquid medium may contain other components such as an acid or an alkali as a pH adjuster and a surfactant as an antifoaming agent, if necessary.

The plant growth regulator of the present invention is preferably
It contains a mixture of the above liquid medium and indolebutyric acid. The amount of indole butyric acid used will be described later. Indolebutyric acid is a compound known as a plant rooting promoter and is commercially available (for example, "Oxyberon (registered trademark)" of Shionogi Pharmaceutical). A mixture obtained by adding indolebutyric acid to a liquid medium for BT culture is particularly effective in obtaining effects such as rooting of plants and promotion of root elongation.

(Preparation of plant growth regulator) The liquid medium used in the present invention is prepared by adding the above-mentioned carbon source, organic nitrogen source, inorganic salt and other optional components to water and dissolving or dispersing them uniformly. Can be prepared as a mixed solution. This mixture is usually heat sterilized for use as a plant growth regulator. Preferably, the mixture is stirred at room temperature after adjusting the pH to near neutral, for example, in the range of about 6.0 to 8.0. In a tank culture or the like, it is preferable to stir while aerating. The stirring time is usually about 24 hours or more, preferably about 30 to 60 hours, more preferably about 40 to 60 hours. After completion of the stirring, the mixture is adjusted to pH
Is preferably adjusted to be acidic, for example, in the range of about 2.0 to 4.0.

Here, "room temperature" means about 10 to 30 ° C.
Preferably, it refers to a temperature in the range of about 15-25 ° C. In addition, the above-mentioned operation of "stirring" refers to a degree of stirring provided to maintain aerobic culture conditions in a culture device usually used for culturing BT bacteria. It should be noted that the efficiency of stirring varies depending on the amount of the mixed liquid to be treated, the capacity of the stirring device, and the like.

The effect of the above-mentioned stirring and pH adjustment on the plant growth regulating action of the liquid medium is not clear. As described above, in the liquid medium, there is a possibility that any component present in the pork powder may interact with or chemically react with the other component. This interaction or chemical reaction may be promoted by stirring (i.e., ripening) under predetermined pH conditions. However, this is not intended to limit the invention to a particular mode of action.

More specifically, the above liquid medium can be prepared by the following series of steps (a) to (e). This preparation can be performed, for example, using equipment provided for culturing BT bacteria as it is.

(A) A preculture medium containing a part of each of the above-mentioned carbon source, organic nitrogen source and inorganic salt is prepared.
After adjusting H to a range of about 6.0 to 8.0, heat sterilization is performed. The preculture medium is usually prepared in an amount of about 2 to 10% based on the total weight of the liquid medium. Heat sterilization is conveniently performed by blowing steam. It is not necessary that pork powder be added to the preculture medium.

(B) a step of stirring the preculture medium obtained in step (a) at room temperature. The stirring here is, for example,
This can be done by shaking on a rotary shaker. The stirring time is usually about 6 hours or more, preferably about 10 to 30 hours.

(C) A step of preparing a main culture medium containing the above-mentioned carbon source, organic nitrogen source and the rest of the inorganic salt, adjusting the pH to a range of about 6.0 to 8.0, and then sterilizing by heating. .

(D) mixing the preculture medium obtained in step (b) with the main culture medium obtained in step (c), and stirring the resulting mixed medium at room temperature. The stirring here is usually performed by stirring with a rotating blade in the tank. The number of times of stirring is usually about 100 to 200 rpm.
It is about. If necessary, an antidepressant is added to suppress the generation of bubbles.

(E) a step of degassing the mixed medium obtained in the step (d) and then adjusting the pH to a range of about 2.0 to 4.0. Degassing can be performed simply by reducing the number of stirrings. To ensure the homogeneity of the liquid medium, adjust the pH
After confirming the above, it is preferable to stop stirring. As described above, a liquid medium suitably used for the plant growth regulator of the present invention can be obtained.

The liquid medium used in the present invention does not require BT cells or components derived therefrom in order to exhibit a plant growth regulating action. Therefore, it is not necessary to add BT cells during the preparation of the liquid medium. However, the BT culture supernatant obtained after removing the layer containing the cultured cells used for BT culture can also be used as the plant growth regulator of the present invention.

In order to obtain a culture supernatant, the above steps (a) to (e) may be applied to BT culture. Usually, the following step (b ′) can be performed instead of the above step (b).

(B ') A step of adding BT to the preculture medium obtained in the step (a), followed by stirring at room temperature. It is the same as step (b) except that BT is added.

Further, the following step (f) may be performed in order to obtain an upper culture solution from the culture solution obtained in the above (e).

(F) a step of removing the culture substratum containing the precipitate generated in the step (e). This precipitate contains almost all of the BT cells and the toxin protein produced.
In order to complete the sedimentation, after adjusting the pH in the range of about 2.0 to 4.0 in step (e), the culture solution is usually left for about 48 hours or more, preferably for about 60 to 80 hours. It is preferable to keep it. The culture supernatant can be separated from the culture supernatant by a suitable means, for example, suction with a pump. Since the separated culture supernatant may contain viable spores of BT, it is preferable to sterilize by heating again.

Both the liquid medium and the culture supernatant obtained as described above can be used as they are as the plant growth regulator of the present invention. On the other hand, in order to obtain a plant growth regulator as a final product, any additional components may be added to the liquid medium or the culture supernatant, if desired. Examples of such optional components include, in addition to indolebutyric acid, known plant growth regulators, special active water, shiitake mushroom mycelium extract, and the like.

The plant growth regulator of the present invention can be provided by packaging the liquid medium or the culture supernatant liquid as it is, or after diluting it to a desired concentration, and then packaging it appropriately. However, the dosage form of the plant growth regulator is not particularly limited, for example,
The liquid medium may be dried by a known means to prepare a powder. Therefore, the “plant growth regulator” here is
It refers to a drug that can be provided as a commercial product at any stage before, during, or after dilution until it is finally applied to a plant.

(Use of Plant Growth Regulator) The plant growth regulator of the present invention can be applied to a desired plant for the purpose of promoting plant growth. Growth-promoting effects include rooting and root elongation, increased root weight, increased above-ground weight, increased plant height, and the like. The plant growth regulator of the present invention is particularly effective in promoting rooting and root elongation in various plants. The plant growth regulator may also have effects such as fruit set stability, fruit enlargement, and increased yield by promoting the growth of various plant organs.

When applied, the plant growth regulator is diluted to an appropriate concentration in advance, if necessary. For example, the liquid medium prepared by the above steps (a) to (e) or the culture supernatant prepared by the above steps (a) to (f) is about 500 to 60,000-fold, preferably about 10 to 10 times.
After dilution by a factor of 00 to 30,000, it can be applied to plants.

When the plant growth regulator contains indolebutyric acid, indolebutyric acid can be added at any stage before, during, or after dilution of the liquid medium or the culture supernatant. The concentration is, for example, about 1% before a commercially available solution containing 0.4% indolebutyric acid is applied to the plant.
It is diluted 1 to 10,000 times, preferably about 1.3 to 1000 times.

The procedure and means for applying the plant growth regulator are not particularly limited, and are appropriately selected according to the dosage form.
For example, in the case of a liquid preparation, the base or the whole of the cuttings or ears of a plant can be immersed in a liquid preparation filled in a container. Alternatively, the liquid agent may be sprayed on the base of cuttings or cuttings. Further, foliage application and soil irrigation may be performed on the whole growing plant.

Plants to which the plant growth regulator of the present invention can be effectively used include monocotyledonous and dicotyledonous plants that are angiosperms, and gymnosperms. Preferably, they are angiosperms, including, but not limited to, Rhododendrons, Coleoptera, Rosaceae, Lepidoptera and Poaceae.

Examples of plants that can significantly obtain the effects of the present invention include: Carabidaceae including carnation and gypsophila; Brassicaceae including Chinese cabbage, cabbage and radish; Rosaceae including roses, apples and pears; chrysanthemum; , Lettuce, safflower and the like; and plants belonging to the family Poaceae including rice, blackgrass, oakgrass, bentgrass, wheat, corn, sugarcane and the like.

Those skilled in the art will recognize the conditions of use of the plant growth regulator, including suitable concentrations, application procedures and means, depending on the type of plant of interest and the degree of intended plant growth. Can be set as appropriate.

[0043]

The present invention will be described more specifically with reference to the following examples. The raw materials used in this example are all commercially available.

(Production Example 1: Preparation of BT culture upper layer solution) BT
Bacteria used for culturing are Bacillus thuring
inensissubsp. aizawai and B
acillus thuringiensis sub
sp. Kurstaki, and the cells were cultured by the same operation. In the following Examples 1 to 9, the BT culture upper layer solution used was obtained by culturing the kurstaki strain.

(A) Pre-culture of strain The strain cultivated on a normal agar medium (slope medium) was cultivated using the raw materials shown in Table 1.

[0046]

[Table 1]

After mixing and dissolving each raw material, the pH was adjusted to 7.0 ± 0.2 using 6N hydrochloric acid. After dispensing the prepared solution into a flask (600 mL × 12), the solution was subjected to steam sterilization to obtain a liquid medium.

The preculture of the liquid medium was performed at a temperature of 28 ± 2 ° C. for 16 hours using a rotary shaker (rotation speed 200 ± 5 rpm, amplitude 1 inch). P after culture
H is 5.8 ± 0.4, TS (solids content; 18
(Solid content ratio in centrifugation at 00G-10min) is 2
It was 4 ± 8%.

(B) Main Culture The inside of a fermentation tank (capacity: 9 m ³ ) equipped with a stirrer was sterilized, and then cultured in this tank using the raw materials shown in Table 2.

[0050]

[Table 2]

After mixing the raw materials, the pH was adjusted to 7.0 ± 0.1 by adding 20% sodium hydroxide. After pH adjustment, steam was directly blown into the tank, and sterilization was performed at a temperature in the tank of 121 ± 1 ° C. for 35 minutes to obtain a main culture medium. After sterilization, the temperature in the tank was adjusted to 28 ° C. ± 2 ° C.

In the main culture, the pre-culture solution (total amount) obtained in (A) was added to the fermentation tank, the temperature in the tank was 28 ± 2 ° C., the aeration was 312 ± ³ Nm ³ / hour, and the pressure was 0.35 ± 0. 05k
It started under the conditions of gf / cm ² G and stirring frequency of 160 ± 3 rpm. During the main culture, if necessary, a separately sterilized antifoaming agent (P-2000) was added to defoam. The culture was terminated when the pH value of the main culture solution became 7.3 or more between 48 hours and 52 hours.

Simultaneously with the completion of the main culture, the aeration was stopped, and the aeration was switched to 80 rpm to perform degassing for 80 minutes. Next, 53 ± 7 kg of 62% sulfuric acid was added to the main culture termination solution to adjust the pH to 2.9 to 3.0. pH adjustment 30
After a minute, the pH was reconfirmed, and after confirming that the pH was within the range of 3.0 ± 0.1, the stirring was stopped.

(C) Precipitation of Toxin Protein and Separation of Culture Supernatant When the pH-adjusted solution is left at room temperature for 70 ± 4 hours, the solid content containing the toxin protein sediments, and the liquid layer (the culture supernatant)
And separated. The sediment occupied about 60% of the total volume of the culture. The separated culture supernatant was transferred to another tank using a pump. The pH of the culture supernatant was 3.0 ± 0.2.

(Production Example 2: Liquid culture medium for BT culture) The steps (A) and (B) in Production Example 1 were carried out in the same manner except that BT bacteria were not added, and about 6500 L
Of a liquid medium was obtained.

(Example 1) Chrysanthemum (Chrysanthe)
um hortorium) on the rooting and root elongation of cuttings of Bacillus thurigiens
Effects of liquid culture medium for is (hereinafter referred to as BT) culture medium and culture supernatant: After the top buds of chrysanthemum (cultivar: Seiyun) have been developed into three developing leaves and stems having a stem length of 5 cm, the cutting base is shown in Table 3. It was immersed in a diluent (reagent solution) for 3 hours (the control was left untreated), inserted into cutting soil (river sand), and maintained in a greenhouse at a day temperature of 30 ° C./night temperature of 20 ° C. As a result of digging and investigating two weeks after the shoots, the liquid medium and the BT culture upper layer liquid increased the number of roots and the root length.

[0057]

[Table 3]

Example 2 Chrysanthemum (Chrysanthe)
um hortorium)
Mixing effect of the culture upper layer solution and Oxyberon solution (0.04% indole-butyric acid): The top buds of chrysanthemum (cultivar: Seimun) were developed into three developing leaves and 5 cm in stem length. Immersed in the diluent (reagent solution) shown in (3) for 3 hours (leave untreated in the control), and inserted into cutting soil (river sand).
The temperature was controlled in a greenhouse at 0 ° C / night temperature of 20 ° C. As a result of digging and investigating two weeks after the shoots, the mixture of the BT culture upper layer solution and the Oxyberon solution increased the number of roots and the root length.

[0059]

[Table 4]

Example 3 Rose (Rosa polya)
Effect of mixing BT culture upper layer solution and oxyberon solution on rooting of cuttings of ntha): 15-cm long shoots of this year's fresh shoots of rose (variety: Randra) are used as cutting ears, and the cutting ear base is shown in Table 5. It was immersed in a diluent (reagent solution) for 24 hours (the control was left untreated), inserted into cutting soil (river sand), and managed in a greenhouse with a day temperature of 25 ° C / night temperature of 20 ° C. As a result of digging and searching on the 22nd day after the cutting, BT
The mixture of the culture supernatant and Oxyberon solution significantly increased the rooting rate, root number and root length.

[0061]

[Table 5]

Example 4 Carnation (Diant)
Effect of BT culture upper layer solution and oxyberon solution on rooting of cutting buds of H. caryophyllus: carnation (variety: pinky grass), the stem length of the raw side branch of this year is 15 cm. Was immersed in a diluent (reagent solution) shown in (1) for 24 hours (leave untreated in the control), inserted into cutting soil (river sand), and managed in a greenhouse at a day temperature of 25 ° C / night temperature of 20 ° C. 22 after the bud
As a result of digging and examining on the day, the mixture of the BT culture upper layer solution and the Oxyberon solution increased the rooting rate, root number, root weight and root length.

[0063]

[Table 6]

Example 5 Zoysia (Zoysia)
matrell Merr. ) On rooting and growth of): Effect of mixing BT culture upper layer solution and oxyberon solution: Placing lawn grass (18 × 30 cm) of black seagrass (cultivar: Chuba black seagrass) in a bat, and one week after planting the green grass, Table 7 Diluent (reagent) shown in
The foliage was sprayed using a pressurized sprayer with an amount of water equivalent to L / 10a (the control was left untreated) and managed in a greenhouse with a day temperature of 30 ° C and a night temperature of 20 ° C. As a result of digging and investigating on the 28th day after planting the upholstered turf, the mixture of the BT culture upper layer solution and the oxyberon solution increased the plant height, the above-ground weight, the root length and the new root weight.

[0065]

[Table 7]

Embodiment 6 Zoysia ja (Zoysia ja)
ponica Steud. ) On rooting and growth of BT culture: Mixing effect of BT culture upper layer solution and Oxyberon solution: Planting a lawn grass (18 × 30 cm) of Noshiba in a bat, and one week after planting the grass lawn, diluent shown in Table 8 (test sample) The chemicals were sprayed with foliage using a pressurized sprayer at a water volume equivalent to 200 L / 10a (controls were left untreated) and managed in a greenhouse with a day temperature of 30 ° C./night temperature of 20 ° C. As a result of digging and investigating on the 28th day after planting the upholstered turf, the mixture of the BT culture upper layer solution and the oxyberon solution increased the plant height, the above-ground weight, the root length and the new root weight.

[0067]

[Table 8]

Example 7 Bentgrass (Agrost)
is spp. Of BT culture upper layer liquid and Oxyberon solution on rooting and growth of): bentgrass made of bentgrass (variety: Pencloth) with 1 / 5000a of Wagner
The plants were planted in pots, and trimming was managed twice a week for 3 months at a cutting height of 2 cm. After 3 months of pruning management, the diluent (reagent solution) shown in Table 9 was applied twice a week at an interval of 200 L / 1.
Spray foliage using a pressurized sprayer with a water amount equivalent to 0a (no treatment in control), day temperature 30 ° C / night temperature 20
It was controlled in a greenhouse at ℃. As a result of digging and investigating on the 28th day after application of the drug, the mixture of the BT culture upper layer solution and the oxyberon solution increased the plant height, the above-ground weight and the root weight.

[0069]

[Table 9]

Example 8 Rice (Oryza sativ)
aL. Mixing effect of BT culture upper layer liquid and oxyberon liquid on rooting and survival of seedling box: 140 g of rice (cultivar: Koshihikari) seed paddy is grown in seedling box (30 × 60 × 3c)
m, about 5 L of soil used), 10 days after sowing, 500 ml of a diluent (reagent) shown in Table 10 per box was irrigated with the soil (untreated in the control), and the day temperature was 30 ° C./day.
It was controlled in a greenhouse at a night temperature of 20 ° C. Two weeks after the soil irrigation treatment, it was dug up and investigated. As a result, the mixture of the BT culture upper layer solution and the Oxyberon solution increased the number of roots and promoted the survival.

[0071]

[Table 10]

(Example 9) Chinese cabbage (Brassica)
campestris L .; ). Effect of mixing BT culture upper layer solution and oxyberon solution on rooting and survival of cell box seedlings: seeding Chinese cabbage (variety: 60 days) seeds in cell tray (128 wells, using cell seedling soil) One week after sowing, 500 diluents (reagents) shown in Table 11 per box were used.
ml was irrigated with soil (the control was left untreated) and maintained in a greenhouse with a day temperature of 30 ° C / night temperature of 20 ° C. Two weeks after the soil irrigation treatment, it was dug up and investigated. As a result, the mixture of the BT culture upper layer solution and the Oxyberon solution increased the root weight and root length, and promoted rooting.

[0073]

[Table 11]

From the above results, a liquid medium for BT culture,
And BT culture supernatants were both shown to be effective for promoting plant growth. In particular, a mixture of liquid medium or culture supernatant and indolebutyric acid significantly promotes rooting and elongation of roots of plants, taking root such as cuttings,
It is understood that this may promote growth and subsequent growth.

[0075]

According to the present invention, a liquid medium for BT culture,
Alternatively, a plant growth regulator containing a BT culture supernatant is provided. The agent is effective for promoting plant growth, especially plant rooting and root elongation.

Claims (11)

[Claims] 1. A BT (Bacillus thuring)
a plant growth regulator comprising a liquid medium containing a carbon source, an organic nitrogen source and an inorganic salt, which is suitable for cultivation of C.iensis). 2. The plant growth regulator according to claim 1, wherein said organic nitrogen source comprises pork powder. 3. The plant growth regulator of claim 1, wherein said carbon source comprises potato starch and said organic nitrogen source comprises pork powder and corn steep liquor. 4. The plant growth regulator according to claim 1, further comprising an antifoaming agent. 5. A pre-culture medium in which the liquid medium contains the following steps: (a) a pre-culture medium containing a part of each of the carbon source, the organic nitrogen source and the inorganic salt;
(B) a step of stirring the pre-culture medium obtained in step (a) at room temperature for about 10 to 30 hours; A step of preparing a main culture medium containing a nitrogen source and the remainder of the inorganic salt, adjusting the pH to a range of about 6.0 to 8.0, and then sterilizing by heating; (d) obtained by step (b). Mixing the preculture medium with the main culture medium obtained in step (c), and stirring the resulting mixed medium at room temperature for about 30 to 60 hours; and (e) obtained in step (d). The plant growth according to any one of claims 1 to 4, wherein the mixed medium is degassed and then the pH is adjusted to a range of about 2.0 to 4.0. Conditioner. 6. The liquid culture medium is a culture supernatant of BT.
The plant growth regulator according to claim 1. 7. The pre-culture medium, wherein the culture supernatant liquid contains the following steps: (a) a pre-culture medium containing a part of each of the carbon source, the organic nitrogen source and the inorganic salt;
(B ') adding BT to the preculture medium obtained in step (a), followed by stirring at room temperature for about 10 to 30 hours; (c) A) preparing a main culture medium containing the carbon source, the organic nitrogen source and the remainder of the inorganic salt, adjusting the pH to a range of about 6.0 to 8.0, and sterilizing by heating; and Mixing the preculture medium obtained in (b ′) and the main culture medium obtained in step (c), and stirring the obtained mixed medium at room temperature for about 30 to 60 hours; (e) step Degassing the mixed medium obtained in (d) and then adjusting the pH to a range of about 2.0 to 4.0; and (f) a lower culture solution containing the precipitate generated in step (e). The plant growth condition according to claim 6, which is obtained by a method comprising: Pills. 8. The plant growth regulator according to claim 1, further comprising indolebutyric acid in addition to the liquid medium. 9. The method according to claim 1, wherein the liquid medium or a mixture of the liquid medium and indolebutyric acid is contained in an amount effective for promoting rooting and / or root elongation of a plant. 2. The plant growth regulator according to item 1. 10. The plant according to claim 10, wherein the plant is a family of the family Coleoptera, Brassicaceae,
The plant growth regulator according to claim 9, which is a plant belonging to the family Rosaceae, Compositae, or Poaceae. 11. The plant according to claim 11, wherein the plant is a rose (Rosa poly).
The plant growth regulator according to claim 10, which is an anth).