JP2000302688A - Prophylactic and therapeutic agent for hepatopathy - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a prophylactic and therapeutic agent for hepatopathy such as chronic active hepatitis, or the like, originating from virus, drug poisoning, alcohol, or the like, having no adverse reaction and a protective effect on lever by using Apocynum venetum L. or an extract of Apocynum venetum L. as an active ingredient. SOLUTION: This agent uses Apocynum venetum L. or the extract with water, or the like, as the active ingredient. All of shrubs, leaves, stems, roots of the Apocynum venetum L. are usable, especially leaves are preferred, raw collected leaves, dried leaves, especially roasted leaves are especially preferably used as the Apocynum venetum L. In the case of using leaves of the Apocynum venetum L., as a raw material, the leaves are roasted like tea-leaf, boiled down with boiling water or soaked in a warm water, or the like, to obtain the water extract of the Apocynum venetum L. and the water extract is administered as liquid, powder, granules or the like.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a prophylactic / therapeutic agent for liver diseases comprising Rafu hemp or an extract of Rafu hemp as an active ingredient.

[0002]

[Prior art] Rabo hemp (scientific name: Apocynum venetum L.)
Is a perennial inhabiting herbaceous plant of the Oleaceae family (commonly called "grass") that is widely distributed in China. In China, since ancient times, Lufu hemp leaves have been used as a substitute for tea leaves, and Rabo hemp tea has also been used as a folk medicine such as antipyretic. In addition, several research institutes in China have been conducting medical and pharmacological studies on Lufu Asaba, which is used as a folk medicine, and reported that it is effective for hypertension, heart failure, bronchitis, edema, etc. Have been. On the other hand, the liver is also called a “silent organ” because the liver has a strong natural healing power and does not show prominent symptoms with a few disorders. Plays an indispensable function in maintaining the life of a child. Although the etiology and pathology of hepatic dysfunction vary widely, the most demanding therapeutic drug development is chronic active hepatitis, which has high medical needs, and is targeted at this disease as a hepatoprotective drug and other causative treatments. Research and development of various therapeutic agents ranging from antiviral agents to immunomodulators have been actively conducted.

[0003]

The treatment of chronic active hepatitis and the like requires the administration of a drug for a long period of time due to its nature, and the side effect becomes a problem. Therefore, development of a better drug for preventing or treating liver disease, which has a high therapeutic effect regardless of the cause of liver disease such as virus, drug poisoning and alcohol, has been awaited.

[0004]

Means for Solving the Problems The present inventors have found that some crude drugs such as assembly have a hepatoprotective effect, but surprisingly, the extract of Rafu hemp leaf has a hepatoprotective effect. The present invention was completed.

[0005]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail. Rabo hemp used in the present invention is the whole shrub or leaves,
Both stems and roots can be used, but leaves are particularly preferred. Further, Rabo hemp may be collected, dried, or roasted, but roasted is particularly preferred. When using Rabo-hemp leaves as a raw material, Rabo-hemp leaves can be roasted into tea leaves, which can be boiled with boiling water or immersed in warm water to obtain a water extract of Rabo-hemp. There is no particular limitation on the form of use of Rafu hemp or the extract of Rafu hemp, but it can be used, for example, in the form of liquid, powder, granules, or the like. Also, by combining Rafu-hemp or Rabo-hima extract with excipients, auxiliaries, additives, etc., for example, liquids, suspensions, syrups, elixirs, extracts, powders, granules, fine granules Agents, tablets,
It can be made into medicines and health foods as capsules and the like.

EXAMPLES Next, the liver-protecting action of the Rabo-Masa leaf extract will be described. [Sample] The following Rabo hemp leaf extract was used. Twice-roasted Chinese rumbo (trade name: Yanlong tea) was extracted with boiling water, lyophilized after extracting 7.8 kg to obtain 1.4 kg of water extract (hereinafter referred to as extract A). .

[Effects on CCl ₄ -induced hepatocellular injury] A group of eight male ddY mice (6 weeks old) was treated with 50 mg / kg or 500 mg / kg of extract A dissolved in physiological saline for 1 day. Once,
After oral administration for one week, at 16 hours, a CCl ₄ / oliveoil mixed solution (1: 1, v / v) 60 μl / animal was intraperitoneally injected. As a control group, physiological saline only was used for the negative control, and po
After administering Silymarin 100mg / kg to the sitive control,
Similarly, a CCl ₄ / olive oil mixture was injected. Blood was collected 2 hours after the administration. The alanine aminotransferase (hereinafter referred to as ALT) value was measured for the collected blood. Table 1 shows the results. The abbreviations in the table have the following meanings. N: CCl ₄ not administered C: negative control Av500: Extract A, 500 mg / kg administration Av50: Extract A, 50 mg / kg administration Sy100: positive control

[0007]

[Table 1]

[Effect on D-galactosamine / LPS-induced liver injury] A group of eight ddY male mice (6 weeks old)
50 mg / kg or 500 mg / k of extract A dissolved in physiological saline
g, once a day, orally for one week, and then intraperitoneally D-galac
Hepatopathy was induced by injection of tosamine (700 mg / kg) and LPS (10 μg / kg). As a control group, only physiological saline was used for the negative control, and Sily was used for the positive control.
After administration of marin 100 mg / kg, D-galactosamine /
LPS was injected. Blood was collected 8 hours after the administration.
The ALT value of the collected blood was measured. Table 2 shows the results. The abbreviations in the table have the following meanings. N: D-galactosamine / LPS not administered C: negative control Av500: Extract A, 500 mg / kg administration Av50: Extract A, 50 mg / kg administration Sy100: Positive control

[0009]

[Table 2]

[0010] The water extract of Rabo hemp leaf was obtained from the results of a chemical liver injury model caused by CCl ₄ (Table 1) and D-galactosamin.
As is clear from the results (Table 2) in the immune liver injury model induced by e / LPS, it has a significant hepatoprotective effect. Therefore, Lufu hemp and extracts of Rafu hemp are useful as agents for preventing and treating liver diseases such as chronic active hepatitis caused by viruses, drug intoxication, alcohol and the like.

Claims (3)

[Claims] (1) A preventive / therapeutic agent for liver disease, comprising Rafu-ma or an extract of Rafu-ma- as an active ingredient. 2. The preventive / therapeutic agent for liver disease according to claim 1, wherein the Rabo-hemp or Rabo-hemp extract is Rabo-hemp leaf or Rabo-hemp leaf extract. 3. The preventive / therapeutic agent for liver disease according to claim 2, wherein Rofu Asaba is roasted.