JP2000256187A - Medicament for treating pig reproductive and respiratory syndrome - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide the subject medicament useful for preventing and treating reproductive difficulties and respiratory disorders of pigs caused by Porcine reproductive and respiratory syncytial(PRRS) virus, by compounding Crystal Violet and Brilliant Blue FCF. SOLUTION: This medicament contains Crystal Violet and/or Brilliant Blue FCF as active ingredients. The pig reproductive and respiratory syndrome is preferably treated or prevented by such a method that a barn or an aimed domestic animal is sprayed with a solution containing the above ingredients (preferably, in an amount of 0.005-0.01% based on the solution) to make the animal enough inhale the sprayed solution.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a drug for reducing the damage of pig reproductive and respiratory disorders in the pig raising industry and a method for preventing and treating the syndrome. More specifically, the present invention relates to a drug containing one or both of crystal violet and brilliant blue FCF as an active ingredient and a method for treating and preventing pig reproductive and respiratory disorders using the same solution.

[0002]

BACKGROUND OF THE INVENTION Pig reproductive and respiratory syndrome (PRRS) is a disease characterized by reproductive disorders in sows and respiratory disorders in piglets. The disease was observed in the United States in 1987, was confirmed in Europe in 1990, and is now widespread worldwide.
The economic loss from this disease is enormous due to miscarriage and stillbirth, the death of suckling and piglets, reduced feed efficiency, and increased drug and labor costs. The disease is caused by PRRS virus infection. In addition, infected pigs carry the virus in the body for a long time (several months) and become a source of infection for other pigs. Once the PRRS virus has entered the farm, the virus will continue to exist on the farm and the damage will continue.

[0003] At present, vaccines for suppressing this disease have been developed, but their effects are not sufficient, and it can be said that there is no effective damage reduction method or prevention method. Heretofore, coloring agents have been used for raising livestock (Japanese Patent Publication No. 58-291).
Publication), but the purpose of use is different. In addition, there have been cases where pigments have been used to combat respiratory disease in pigs and respiratory disease in cattle, and all of them have been reported to be effective against general bacteria. There is no suggestion about this (Clinical Veterinary, Vol. 7, No. 2, 1989, 84).

[0004]

As described above, in the prior art, there is no method for suppressing the damage of PRRS. An object of the present invention is to provide a method of cutting off the infection cycle of the PRRS virus continuously circulating between herds and suppressing the damage of PRRS.

[0005]

In order to solve the above problems, the present invention has found that crystal violet and brilliant blue FCF have strong virucidal activity against PRRS virus, and utilize this virucidal effect. Has developed a method to dramatically reduce the damage of PRRS.

[0006] The PRRS damage reducing agent used in the present invention can be obtained by the method described in the following Examples.

Example 1 Method for Preparing a Drug Preparation Method for a 4% Crystal Violet Solution: To 20 g of crystal violet powder was added 80 ml of ethanol.
The mixture was stirred with a magnetic stirrer for 30 minutes to completely dissolve. To this, 416 ml of purified water was added with stirring for 30 minutes to obtain a 4% crystal violet solution having a total volume of 500 ml. Preparation method of 4% brilliant blue FCF solution: 80 ml of ethanol was added to 20 g of brilliant blue FCF powder, and the mixture was stirred with a magnetic stirrer for 30 minutes to completely dissolve. To this, 416 ml of purified water was added with stirring for 30 minutes to obtain a total of 500 ml of a 4% brilliant blue FCF solution. Preparation of 2% Crystal Violet / 2% Brilliant Blue FCF Mixed Solution: The above 4% crystal violet solution was prepared by mixing 250 ml of the above 4% brilliant blue FCF solution.

Example 2 Killing action of drugs on PRRS virus From a 4% crystal violet solution and a 4% brilliant blue FCF solution, 0.1, 1.0, 5.0,
Adjusted to 10.0 and 20.0 mcg / ml. 1 ml of the drug solution adjusted to each concentration and 1 ml of the PRRS virus solution were mixed and mixed.
Acted at 7 ° C. for 10 minutes. Next, the drug mixture of each concentration and the virus mixture were immediately and serially diluted 10-fold, and cells susceptible to PRRS virus (MARC-145 cells were inoculated and the virus titer was measured. As shown in Table 1, the concentration of each drug killed in Crystal Violet was 1 mcg / ml or more, and Brilliant Blue FC
F had a concentration of 10 mcg / ml or more.

[Table 1]

Example 3 Effect of 0.01% Crystal Violet Solution on PRRS Damage Reduction by Spray Aspiration Treatment The farm where this test was conducted was a PRRS-contaminated farm. He was spending a lot of effort and money on the daily treatment of pigs. At this farm, a spray-inhalation treatment of 0.01% crystal violet solution was performed to reduce the number of pigs with PRRS. 4% crystal violet solution was diluted 400 times with tap water on the farm to 0.01%
Adjusted to solution. A 0.01% crystal violet solution was sprayed on the head of a weaned piglet using a sprayer. As the piglets breathed, the nebulized drug was sprayed into the piglets' nasal cavity, trachea and lungs so as to be inhaled. The spray aspiration treatment of the drug was performed once a day. As a result, as shown in Table 2, out of the 166 weaned piglets in the untreated group, the number of animals treated with respiratory symptoms reached an average of 45 a day.
On the other hand, in the group in which the spray-inhalation treatment of the drug was performed, the average number of piglets exhibiting respiratory symptoms was 3 per day, and none of the piglets required treatment. As described above, piglets that showed respiratory symptoms due to the PRRS virus and required treatment could be drastically reduced by spraying the drug.

[Table 2]

Example 4 Effect of PRRS Damage Reduction by Spray-Aspiration Treatment of 0.01% Crystal Violet Solution The farm where the test was conducted was a PRRS-contaminated farm, and in particular, weaned piglets showed respiratory symptoms and died. It was frequent and severely damaged. At this farm, a 0.01% crystal violet solution was sprayed and aspirated to reduce the number of pigs with PRRS and the number of dead pigs. Apply 4% crystal violet solution using farm tap water for 400
It was double-diluted and adjusted to a 0.01% crystal violet solution. The 0.01% solution was sprayed on the head of weaned piglets using a sprayer. As the piglets breathed, the nebulized drug was sprayed into the piglets' nasal cavity, trachea and lungs so as to be inhaled. The spray aspiration treatment of the drug was performed once a day. As a result, as shown in Table 3, in the untreated group (results in the two months before the administration of the spray inhalation of the drug), 19 out of 1413 weaned piglets
Three died of respiratory symptoms. On the other hand, 1242
34 weaned piglets died. As mentioned above, PR
The piglets that died due to the RS virus could be drastically reduced by spraying the drug.

[Table 3]

EXAMPLE 5 The effect of reducing PRRS damage by spray suction treatment of a 0.01% brilliant blue FCF solution was tested. The farm on which the test was conducted was a PRRS-contaminated farm, and in particular, the piglets exhibited respiratory symptoms and died frequently, causing severe damage. In this farm, PR
For the purpose of reducing the number of pigs with RS and the number of dead pigs, spray suction treatment of a 0.01% brilliant blue FCF solution was performed. The 4% brilliant blue FCF solution was diluted 400-fold with tap water on the farm to prepare a 0.01% brilliant blue FCF solution. The 0.01% solution was sprayed on the head of suckling pigs and weaned piglets using a sprayer. The treatment was performed so that the nebulized drug was sucked into the nasal cavity, trachea, and lungs of the piglets by the respiration of the piglets. The spray suction treatment of the drug was performed once a day. As a result, as shown in Table 4, 14 out of 82 suckling pigs died in the untreated group, and 7 out of 69 weaned piglets died of respiratory symptoms. On the other hand, one of the 65 suckling pigs died and 64 weaned piglets had no deaths in the group that received the drug spray treatment. As described above, spraying and sucking a drug on piglets that die due to the PRRS virus has the effect of drastically reducing the mortality.

[Table 4]

Example 6 PRRS by spraying and suctioning a mixed solution of 0.005% crystal violet and 0.005% brilliant blue FCF
Damage reduction effect The farm where the test was conducted was a PRRS-contaminated farm, especially where suckling pigs and weanling piglets frequently died due to respiratory symptoms.
He was severely damaged. At this farm, 0.0% of pigs with PRRS
A spray suction treatment of a mixed solution of 05% crystal violet and 0.005% brilliant blue FCF was performed. 4% crystal violet solution and 4% brilliant blue FCF
The solutions were mixed in equal volumes and diluted 400-fold with tap water on the farm to prepare a 0.005% mixed solution. The mixed solution was sprayed with a sprayer on the heads of suckling pigs and weaned piglets. Treatment was performed so that the nebulized drug was inhaled into the nasal cavity, trachea and lungs of the piglets by the piglets breathing.
These treatments were performed once daily. As a result, as shown in Table 5, in the untreated group, 20 of the 152 suckling pigs died, and 9 of the 171 weanling piglets died of respiratory symptoms. On the other hand, 16
There were 0 deaths in 9 suckling pigs and 135 weaned piglets. As described above, the effect of drastically reducing the number of piglets that died due to the PRRS virus by spraying and suctioning this drug was obtained.

[Table 5]

[Effect] As described above, the method of spraying and sucking the agent of the present invention into swine has an effect of dramatically suppressing the occurrence of PRRS and remarkably reducing the onset death. As a result, enormous economic effects can be produced.

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[Procedure amendment]

[Submission date] March 7, 2000 (200.3.7)

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] Full text

[Correction method] Change

[Correction contents]

[Document Name] Statement

[Title of the Invention] Agent for treating pig reproductive and respiratory disorders

[Claims]

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a drug for reducing the damage of pig reproductive and respiratory disorders in the pig raising industry and a method for preventing and treating the syndrome. More specifically, the present invention relates to a drug containing brilliant blue FCF as an active ingredient and a method for treating and preventing porcine reproductive and respiratory distress syndrome using the same.

[0002]

BACKGROUND OF THE INVENTION Pig reproductive and respiratory syndrome (PRRS) is a disease characterized by reproductive disorders in sows and respiratory disorders in piglets. The disease was observed in the United States in 1987, was confirmed in Europe in 1990, and is now widespread worldwide.
The economic loss from this disease is enormous due to miscarriage and stillbirth, the death of suckling and piglets, reduced feed efficiency, and increased drug and labor costs. The disease is caused by PRRS virus infection. In addition, infected pigs carry the virus in the body for a long time (several months) and become a source of infection for other pigs. Once the PRRS virus has entered the farm, the virus will continue to exist on the farm and the damage will continue.

[0003] At present, vaccines for suppressing this disease have been developed, but their effects are not sufficient, and it can be said that there is no effective damage reduction method or prevention method. Heretofore, coloring agents have been used for raising livestock (Japanese Patent Publication No. 58-291).
Publication), but the purpose of use is different. In addition, there have been cases where pigments have been used to combat respiratory disease in pigs and respiratory disease in cattle, and all of them have been reported to be effective against general bacteria. There is no suggestion about this (Clinical Veterinary, Vol. 7, No. 2, 1989, 84).

[0004]

As described above, in the prior art, there is no method for suppressing the damage of PRRS. In addition, some pigments are suspected to be carcinogenic and mutagenic in animals, and their safety is concerned (MUTAT. RES. 58 (2, 3) 26
9, 276 (1978)). An object of the present invention is to provide a method of cutting off the infection cycle of the PRRS virus continuously circulating between herds and suppressing the damage of PRRS.

[0005]

Means for Solving the Problems In order to solve the above problems, the present invention has found that brilliant blue FCF has a strong virucidal activity against PRRS virus. A method has been developed to dramatically reduce damage. The brilliant blue FCF used in the present invention is designated as a food additive and is advantageous in terms of safety (Fd. Chem. Toxic. 28, 221, 1990).

[0006] The PRRS damage reducing agent used in the present invention can be obtained by the method described in the following Examples.

Example 1 Method for Preparing a Drug Preparation Method for a 4% Crystal Violet Solution: To 20 g of crystal violet powder was added 80 ml of ethanol.
The mixture was stirred with a magnetic stirrer for 30 minutes to completely dissolve. To this, 416 ml of purified water was added with stirring for 30 minutes to obtain a 4% crystal violet solution having a total volume of 500 ml. Preparation method of 4% brilliant blue FCF solution: 80 ml of ethanol was added to 20 g of brilliant blue FCF powder, and the mixture was stirred with a magnetic stirrer for 30 minutes to completely dissolve. To this, 416 ml of purified water was added with stirring for 30 minutes to obtain a total of 500 ml of a 4% brilliant blue FCF solution. Preparation of 2% Crystal Violet / 2% Brilliant Blue FCF Mixed Solution: The above 4% crystal violet solution was prepared by mixing 250 ml of the above 4% brilliant blue FCF solution.

Example 2 Killing action of drugs on PRRS virus From a 4% crystal violet solution and a 4% brilliant blue FCF solution, 0.1, 1.0, 5.0,
Adjusted to 10.0 and 20.0 mcg / ml. 1 ml of the drug solution adjusted to each concentration and 1 ml of the PRRS virus solution were mixed and mixed.
Acted at 7 ° C. for 10 minutes. Next, the drug mixture of each concentration and the virus mixture were immediately and serially diluted 10-fold, and cells susceptible to PRRS virus (MARC-145 cells were inoculated and the virus titer was measured. As shown in Table 1, the concentration of each drug killed in Crystal Violet was 1 mcg / ml or more, and Brilliant Blue FC
F had a concentration of 10 mcg / ml or more.

[Table 1]

Example 3 The PRRS damage reduction effect by spray suction treatment of a 0.01% brilliant blue FCF solution was tested. The farm on which the test was conducted was a PRRS-contaminated farm, and in particular, the piglets exhibited respiratory symptoms and died frequently, causing severe damage. In this farm, PR
For the purpose of reducing the number of pigs with RS and the number of dead pigs, spray suction treatment of a 0.01% brilliant blue FCF solution was performed. The 4% brilliant blue FCF solution was diluted 400-fold with tap water on the farm to prepare a 0.01% brilliant blue FCF solution. The 0.01% solution was sprayed with a sprayer on the heads of suckling pigs and weaned piglets. The treatment was performed so that the nebulized drug was sucked into the nasal cavity, trachea, and lungs of the piglets by the respiration of the piglets. The spray suction treatment of the drug was performed once a day. As a result, as shown in Table 4, 14 out of 82 suckling pigs died in the untreated group, and 7 out of 69 weaned piglets died of respiratory symptoms. On the other hand, one of the 65 suckling pigs died and 64 weaned piglets had no deaths in the group that received the drug spray aspiration treatment. As described above, spraying and sucking a drug on piglets that die due to the PRRS virus has the effect of drastically reducing the mortality.

[Table 2]

Example 4 PRRS by spraying and suctioning a mixed solution of 0.005% crystal violet and 0.005% brilliant blue FCF
Damage reduction effect The farm where the test was conducted was a PRRS-contaminated farm, especially where suckling pigs and weanling piglets frequently died due to respiratory symptoms.
He was severely damaged. At this farm, 0.0% of pigs with PRRS
A spray suction treatment of a mixed solution of 05% crystal violet and 0.005% brilliant blue FCF was performed. 4% crystal violet solution and 4% brilliant blue FCF
The solutions were mixed in equal volumes and diluted 400-fold with tap water on the farm to prepare a 0.005% mixed solution. The mixed solution was sprayed with a sprayer on the heads of suckling pigs and weaned piglets. Treatment was performed so that the nebulized drug was inhaled into the nasal cavity, trachea and lungs of the piglets by the piglets breathing.
These treatments were performed once daily. As a result, as shown in Table 3, in the untreated group, 20 out of 152 suckling pigs died, and 9 out of 171 weaned piglets died due to respiratory symptoms. On the other hand, 16
There were 0 deaths in 9 suckling pigs and 135 weaned piglets. As described above, the effect of drastically reducing the number of piglets that died due to the PRRS virus by spraying and suctioning this drug was obtained.

[Table 3]

[Effect] As described above, the method of spraying and sucking the agent of the present invention into swine has an effect of dramatically suppressing the occurrence of PRRS and remarkably reducing the onset death. As a result, enormous economic effects can be produced.

 ────────────────────────────────────────────────── ─── Continued on the front page (72) Inventor Hiroshi Tanabe 2-2-15 Taki, Kamifukuoka-shi, Saitama F-term (reference) 4C206 AA01 AA02 FA41 MA01 MA02 MA04 MA33 MA36 ZC61

Claims (3)

[Claims] 1. An agent for treating and preventing swine reproductive and respiratory disorders, comprising one or both of crystal violet and brilliant blue FCF as active ingredients. 2. A swine breeding / respiratory disorder syndrome, which comprises spraying a solution containing one or both of crystal violet and brilliant blue FCF as an active ingredient onto a barn or a target animal, and allowing the solution to be sufficiently aspirated. Treatment or prevention method. 3. The method according to claim 2, wherein a solution having a concentration of 0.005-0.01% is sprayed.