JP2000198998A - Bactericidal detergent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a bactericidal detergent which is excellent in bactericidal and cleaning effects and in safeness by incorporating bile acid, an organic acid, and an alcohol into the same. SOLUTION: Bile acid used maybe in the form of a bile powder, a crude product, separated from bile, etc., of cattle or pigs or may be a high-purity one obtained by purifying a crude product. Cholic acid and deoxycholic acid separated from bile acid may be used in place of bile acid. The concentration of bile acid in the detergent is 0.05-5 wt.%. Though the organic acid may be arbitrarily selected, a 2-6C mono- or polycarboxylic acid is preferably used, such as acetic, citric, or lactic acid. The cocentration of the organic acid in the detergent is 0.05-4 wt.%. The alcohol is a 2-6C monoalcohol, preferably ethanol, and its concentration in the detergent is 10-30 wt.%. When used, the detergent generally has a pH of 3-5.5. A natural-derived acid (e.g. citric acid) or its salt, carbonate, or phosphate is used as an adjusting agent.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a highly safe sterilizing and cleaning agent which exhibits a high bactericidal effect on bacteria such as vegetables and fruits, food production equipment, and bacteria attached to tableware.

[0002]

2. Description of the Related Art In foods, food production equipment, and tableware, control of microorganisms has been a major problem, and various methods have been proposed. For example, in the case of food, heat sterilization, ultrahigh pressure sterilization, washing and removal, and the like have been performed as physical methods. In the former two cases, the food itself may have adverse effects such as discoloration and change in flavor. Chemically, various sterilizing preservatives have been developed and are being used effectively. However, sometimes the adverse effect on the eater is aimed at, and a highly safe preservative is desired. In many cases, these methods are employed in a combined form to comprehensively control microorganisms.

For example, JP-A-6-279785 discloses a detergent composition containing a monoglyceride polycarboxylic acid ester and fumaric acid and / or succinic acid and / or maleic acid. Also, JP-A-6-2
JP 79787 discloses a detergent composition containing monoglyceride polyvalent carboxylic acid ester and polyphosphoric acid, which is effective for washing and removing bacteria, but the washing component used here is a chemically synthesized product. Therefore, a composition that can effectively remove bacteria with only safer natural components is desired.

Further, Japanese Patent Laid-Open No. 50-145521,
Japanese Unexamined Patent Publication (Kokai) No. 2-21856 discloses a food preservative in which cholic acid or a salt thereof is combined with condensed phosphate or protamine. Kaihei 7-277986
In the publication, an antibacterial agent for methicillin-resistant Staphylococcus aureus containing deoxycholic acid / salt as a main component is disclosed.
Although they are considered to be relatively safe, they have a problem in the effect on the flavor of foods. Originally, it is better that such a substance does not coexist in food, and a better method of controlling microorganisms has been desired.

[0005] The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a germicidal detergent having a high effect of disinfecting and cleaning microorganisms and having excellent safety.

[0006]

Means for Solving the Problems The present inventors have found that the above problems can be solved by using a sterilizing detergent containing bile acids, organic acids and alcohols, and have completed the present invention. .

[0007]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail.
The bile acid used in the present invention may be a bile powder of a crude product separated from bovine or porcine bile or the like, and more preferably a highly purified bile powder. Separated cholic acid or deoxycholic acid can also be used. These production methods may be ordinary methods and are not particularly limited. The concentration of bile acid in the washing solution is generally 0.05% by weight to 5% by weight.
And preferably 0.05 to 2% by weight.

The organic acid used in the present invention can be arbitrarily selected, but is preferably a mono- or polyvalent carboxylic acid having 2 to 6 carbon atoms, such as acetic acid, citric acid or lactic acid.
More preferred is acetic acid. The concentration of the organic acid in the washing solution is generally 0.05% to 4% by weight, preferably 0.05% to 2%.

[0009] The alcohol used in the present invention is a monoalcohol having 2 to 6 carbon atoms, preferably ethanol. The concentration of alcohol in the cleaning solution is generally 1
It is 0% to 30% by weight, preferably 10% to 25% by weight.

The pH of the sterilizing detergent of the present invention at the time of use is generally from 3 to 5.5, preferably from 3.5 to 5. p
Examples of the H regulator include agents used for keeping food in an appropriate pH range, such as adipine, citric acid (crystal or anhydrous), trisodium citrate, glucono delta lactone, gluconic acid, succinic acid, and succinic acid. Monosodium, disodium succinate, sodium acetate (crystal or anhydrous), DL-tartaric acid, DL-sodium tartrate, L-tartaric acid, L-sodium tartrate, carbon dioxide, lactic acid, sodium lactate, glacial acetic acid, fumaric acid, fumaric acid Monosodium, DL-malic acid, DL-sodium malate, phosphoric acid, DL-potassium hydrogen tartrate, L-potassium hydrogen tartrate, potassium carbonate (anhydrous), sodium carbonate (crystal or anhydrous), sodium bicarbonate, hydrogen hydrogen phosphate Dipotassium, potassium dihydrogen phosphate, disodium hydrogen phosphate (crystalline or anhydrous), phosphorus Sodium dihydrogen (crystal or anhydrous), disodium dihydrogen pyrophosphate, itaconic acid, alpha-ketoglutaric acid (extract), etc. phytic acid. Of these, citric acid and L-
Naturally occurring acids such as tartaric acid and lactic acid, and salts, carbonates, and phosphates thereof. One or two of these
Adjust the pH by mixing more than one species. The total concentration of this pH adjuster is from 0.3% by weight to 10% by weight, preferably from 1% by weight to
5% by weight. If it is less than 0.3% by weight, the pH buffering capacity is weak, and if it exceeds 10% by weight, it is not preferable in terms of cost.

Further, other components may be added to the germicidal detergent of the present invention, if necessary. For example, antimicrobial agents usable for food additives such as polylysine, catechin, protamine and spice extract, commonly used hydrotropes such as glycerin and other polyhydric alcohols, pigments usually used for food, organic calcium and ascorbine Inorganic and organic nutrients commonly used in foods such as acids, emulsifiers usually used in foods such as saponin, salts, odor and taste modifiers usually used in foods such as cyclodextrin and trehalose, xanthan gum and carrageenan And viscosity modifiers usually used for foods such as gelatin.

The germicidal detergent of the present invention is used in detergent solutions of the above-mentioned respective component amounts. The state before being made into a detergent solution may be any of paste, liquid and solid. It is manufactured by mixing the respective components by the method described above. At this time, it is heated if necessary. In the case of a liquid detergent, filtration is performed as necessary.

The germicidal detergent of the present invention is used by appropriately dissolving it in water, and after immersing vegetables, fruits and dishes in the detergent solution, rinsing with running water or running water. Alternatively, the solution may be directly sprayed on vegetables, fruits and tableware, and then rinsed with running water or stored water. This solution can also be used for cleaning food manufacturing equipment.

[0014]

EXAMPLES Hereinafter, the present invention will be described with reference to specific examples for clarifying the effects of the present invention, but the present invention is not limited to these examples. In the following examples, all percentages are by weight. (Examples 1 to 4, Comparative Examples 1 to 4) Cleaning solutions were prepared using water as a balance so as to have the concentrations shown in Tables 1 and 2. The following bactericidal activity test was performed using this detergent solution.

(Test method for bactericidal activity) About 20 g of commercially available cucumber
Was pulverized with a stomacher for 2 minutes together with 80 g of physiological saline, and a liquid obtained by filtration was used as an evaluation bacterial liquid. 10 ml of the detergent solution shown in Table 1 was collected in a sterile test tube, and 1 ml of the evaluation bacterial solution was added thereto, and the mixture was allowed to stand for 1 minute. Next, the sterilization test was stopped by collecting 0.5 ml of the treated solution and diluting it into 4.5 ml of physiological saline. According to a conventional method, this solution was serially diluted with physiological saline, spread on a petri dish, cultured in an SPC medium at 32 ° C. for 3 days, and then the number of bacteria was measured to evaluate the bactericidal activity. Examples and comparative examples are shown in Tables 1 and 2.

[0016]

[0017]

Example 5 0.2% cholic acid and 0.1% acetic acid.
A detergent solution of 3%, ethanol 20%, and water balance was prepared. The solution is adjusted to pH using sodium citrate.
It was adjusted to 5.0. 500 g of mini tomato was immersed in 5 liters of this solution for 10 minutes and rinsed with running water for 1 minute. 50 g of this mini tomato is ground together with 50 g of physiological saline,
Filtration yielded a bacterial solution. This bacterial solution is serially diluted with a physiological saline in accordance with a conventional method, and spread on a petri dish.
After culturing at 2 ° C. for 3 days, the bactericidal activity was evaluated by measuring the number of bacteria. As a result of the measurement, the number of general viable bacteria was 10 or less / 1 g of mini tomato, and a sufficient bactericidal effect was recognized. In addition, when only water was used instead of the above-mentioned washing solution and the same procedure was carried out except for the above, the general viable cell count was 1.3 × 10 ³ cells / g of mini tomato.

(Example 6) 0.2% of bile powder, 0.3 of acetic acid
%, Ethanol 20%, saponin 0.05%, a water balance detergent solution was prepared. This solution was adjusted to pH 5.0 using sodium citrate. 10 g of this solution was sprayed on 1 kg of broccoli using a sprayer,
After standing for 1 minute, it was rinsed with running water for 1 minute. 20 g of this broccoli was ground together with 100 g of physiological saline and filtered to obtain a bacterial solution. This bacterial solution was serially diluted with a physiological saline in accordance with a conventional method, spread on a petri dish, cultured in an SPC medium at 32 ° C. for 3 days, and then the number of bacteria was measured to evaluate the bactericidal activity. As a result of the measurement, the number of general viable bacteria was 3.8 × 10 ² cells / g of broccoli, and a sufficient bactericidal effect was recognized. In addition, when only water was used in place of the above-mentioned washing solution and the same procedure was carried out except for the above, the general viable cell count was 2.3 × 10 ⁵ / g of broccoli.

[0020]

As described above, according to the present invention,
Suitable for sterilization cleaning of foods, food production equipment and tableware,
A germicidal detergent with high safety and excellent bactericidal effect can be obtained.

Claims (1)

[Claims] 1. A germicidal detergent containing a bile acid, an organic acid and an alcohol.