JP2000139455A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2000139455A5 JP2000139455A5 JP1999295808A JP29580899A JP2000139455A5 JP 2000139455 A5 JP2000139455 A5 JP 2000139455A5 JP 1999295808 A JP1999295808 A JP 1999295808A JP 29580899 A JP29580899 A JP 29580899A JP 2000139455 A5 JP2000139455 A5 JP 2000139455A5
- Authority
- JP
- Japan
- Prior art keywords
- medium
- growth
- islands
- culture
- islets
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002609 medium Substances 0.000 description 18
- 238000000034 method Methods 0.000 description 14
- 239000011159 matrix material Substances 0.000 description 10
- 210000004153 islets of langerhan Anatomy 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 8
- 210000002950 fibroblast Anatomy 0.000 description 8
- 239000007640 basal medium Substances 0.000 description 7
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 7
- 210000002966 serum Anatomy 0.000 description 7
- 210000003890 endocrine cell Anatomy 0.000 description 6
- 239000003102 growth factor Substances 0.000 description 6
- 238000011534 incubation Methods 0.000 description 6
- 108010025020 Nerve Growth Factor Proteins 0.000 description 5
- 102000015336 Nerve Growth Factor Human genes 0.000 description 5
- 229940053128 nerve growth factor Drugs 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 102000004877 Insulin Human genes 0.000 description 4
- 108090001061 Insulin Proteins 0.000 description 4
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 4
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 4
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 229940125396 insulin Drugs 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- 230000035755 proliferation Effects 0.000 description 3
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 229930195725 Mannitol Natural products 0.000 description 2
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 2
- 108010038512 Platelet-Derived Growth Factor Proteins 0.000 description 2
- 102000010780 Platelet-Derived Growth Factor Human genes 0.000 description 2
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 2
- 241000700159 Rattus Species 0.000 description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 description 2
- 108010012715 Superoxide dismutase Proteins 0.000 description 2
- 108090000190 Thrombin Proteins 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 235000010355 mannitol Nutrition 0.000 description 2
- 239000000594 mannitol Substances 0.000 description 2
- 230000001817 pituitary effect Effects 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 229960004072 thrombin Drugs 0.000 description 2
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 2
- 102000009024 Epidermal Growth Factor Human genes 0.000 description 1
- 101800003838 Epidermal growth factor Proteins 0.000 description 1
- 101001076292 Homo sapiens Insulin-like growth factor II Proteins 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 102000004218 Insulin-Like Growth Factor I Human genes 0.000 description 1
- 102000048143 Insulin-Like Growth Factor II Human genes 0.000 description 1
- 108090001117 Insulin-Like Growth Factor II Proteins 0.000 description 1
- 102100025947 Insulin-like growth factor II Human genes 0.000 description 1
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 230000000421 anti-necrotic effect Effects 0.000 description 1
- 229940116977 epidermal growth factor Drugs 0.000 description 1
- 229960000890 hydrocortisone Drugs 0.000 description 1
- 230000002608 insulinlike Effects 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229960003387 progesterone Drugs 0.000 description 1
- 239000000186 progesterone Substances 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR43491/1998 | 1998-10-17 | ||
| KR1019980043491A KR19990014353A (ko) | 1998-10-17 | 1998-10-17 | 췌도의 대량 배양증식방법 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2000139455A JP2000139455A (ja) | 2000-05-23 |
| JP2000139455A5 true JP2000139455A5 (enExample) | 2005-12-08 |
Family
ID=19554380
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11295808A Pending JP2000139455A (ja) | 1998-10-17 | 1999-10-18 | ランゲルハンス島の培養及び島自己移植島再生方法 |
Country Status (7)
| Country | Link |
|---|---|
| EP (2) | EP1344818A1 (enExample) |
| JP (1) | JP2000139455A (enExample) |
| KR (1) | KR19990014353A (enExample) |
| AT (1) | ATE284952T1 (enExample) |
| AU (1) | AU775691B2 (enExample) |
| CA (1) | CA2286530A1 (enExample) |
| DE (1) | DE69922610T2 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6592618B2 (en) | 2000-02-29 | 2003-07-15 | The Brigham And Women's Hospital, Inc. | In vitro modification of cardiac valvular xenografts |
| DE10134667A1 (de) * | 2001-07-20 | 2003-02-06 | Neuroprogen Gmbh Leipzig | Verfahren zur Herstellung isolierter Zellkulturen, Kulturmedium zur Kultivierung von Zellkulturen und Zellkultur |
| PT1636348E (pt) * | 2003-06-20 | 2009-10-21 | Vrije Universiteit Brussel Vub | Método de gerar ilhotas de células beta a partir de células pancreáticas exócrinas |
| AU2004295702A1 (en) * | 2003-12-01 | 2005-06-16 | Vertex Pharmaceuticals Incorporated | Compositions comprising fetal liver cells and methods useful for HCV infection |
| WO2005086860A2 (en) * | 2004-03-09 | 2005-09-22 | Gang Xu | Methods for generating insulin-producing cells |
| KR100855104B1 (ko) * | 2006-06-28 | 2008-08-29 | 피더블유제네틱스코리아 주식회사 | 췌도세포의 장기 보존을 위한 취급방법 |
| US20090060843A1 (en) * | 2007-08-31 | 2009-03-05 | Biocrine Ab | Non-Invasive In Vivo Imaging and Methods for Treating Type I Diabetes |
| KR101092411B1 (ko) * | 2009-07-22 | 2011-12-09 | (주)시지바이오 | 이식용 동종 피부의 처리 방법 및 그로부터 제조된 동결보존동종피부 |
| EP2546334A4 (en) * | 2010-03-12 | 2014-10-29 | Fundación Progreso Y Salud | METHOD FOR THE IN VITRO PROLIFERATION OF TISSUE DERIVED FROM TISSUE ENDORMALLY ORIGINATED CELLS |
| KR101327630B1 (ko) * | 2012-03-05 | 2013-11-13 | 울산대학교 산학협력단 | 아텔로콜라겐을 이용한 췌도세포 이식용 담체의 제조방법 그리고 이를 이용하여 제조된 인공췌장 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE456164B (sv) * | 1980-08-20 | 1988-09-12 | Kjell Nilsson | Forfarande for immobilisering, odling och efterfoljande frigoring av animala celler samt mikroberare av gelatin med absorberade animala celler |
| EP0363125A3 (en) * | 1988-10-03 | 1990-08-16 | Hana Biologics Inc. | Proliferated pancreatic endocrine cell product and process |
| US5116753A (en) * | 1991-07-30 | 1992-05-26 | The Salk Institute For Biological Studies | Maintenance of pancreatic islets |
| US5587309A (en) * | 1994-04-29 | 1996-12-24 | The United States Of America As Represented By The Department Of Health And Human Services | Method of stimulating proliferation and differentiation of human fetal pancreatic cells ex vivo |
| WO1997016536A1 (en) * | 1995-10-30 | 1997-05-09 | Vivorx, Inc. | Method for ex vivo proliferation and differentiation of adult pancreatic islet cells, media useful therefor and uses thereof |
-
1998
- 1998-10-17 KR KR1019980043491A patent/KR19990014353A/ko active Pending
-
1999
- 1999-10-15 CA CA002286530A patent/CA2286530A1/en not_active Abandoned
- 1999-10-18 JP JP11295808A patent/JP2000139455A/ja active Pending
- 1999-10-18 EP EP03013538A patent/EP1344818A1/en not_active Withdrawn
- 1999-10-18 EP EP99308203A patent/EP0994185B1/en not_active Expired - Lifetime
- 1999-10-18 AU AU55930/99A patent/AU775691B2/en not_active Ceased
- 1999-10-18 DE DE69922610T patent/DE69922610T2/de not_active Expired - Fee Related
- 1999-10-18 AT AT99308203T patent/ATE284952T1/de not_active IP Right Cessation
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2577114B2 (ja) | 細胞培養基 | |
| US20060110825A1 (en) | Process for the preparation of stem cells from human muscle tissue and adipose tissue, and stem cell obtainable by this process | |
| KR100907248B1 (ko) | 분화된 어린 지방 세포와 생분해성 중합체의 이식에 의한신체의 부피 대체 방법 | |
| US8609408B2 (en) | Method for the reconstruction of a tissue-engineered human corneal endothelium | |
| WO2021254296A1 (zh) | 一种生物活性物质组合物、包含所述组合物的无血清培养基及其用途 | |
| AU2011236653B2 (en) | Chondrogenic progenitor cells, protocol for derivation of cells and uses thereof | |
| JP2002529071A (ja) | 軟骨細胞様細胞のための無血清培地 | |
| US7037721B1 (en) | Protein-free defined media for the growth of normal human keratinocytes | |
| CN113692282B (zh) | 一种生物活性物质组合物、包含所述组合物的无血清培养基及其用途 | |
| WO2008073331A2 (en) | Method of generation and expansion of tissue-progenitor cells and mature tissue cells from intact bone marrow or intact umbilical cord tissue | |
| US7186558B2 (en) | Keratinocytes obtained from embryonic stem cells of mammals | |
| AU2011236653A1 (en) | Chondrogenic progenitor cells, protocol for derivation of cells and uses thereof | |
| CN109749997B (zh) | 一种角膜缘干细胞无血清培养基及其培养方法 | |
| CN112553148A (zh) | 一种用于培养肉种子细胞体外扩大培养的改良培养基及其应用 | |
| JP2000139455A5 (enExample) | ||
| CN106479978A (zh) | 一种神经干细胞的专用培养基及其培养方法 | |
| US20240124843A1 (en) | Functional feline pancreatic cells from adipose tissue | |
| CN107129966B (zh) | 一种含血清的角膜上皮细胞培养液 | |
| US20050019310A1 (en) | Method for culturing and expansion of mammalian undifferentiated epidermal kerainocytes exhibiting stem cell characteristics | |
| JP2006000059A (ja) | 細胞外基質を用いた動物細胞の増殖及び分化促進方法 | |
| JP2007000077A (ja) | 付着性動物細胞の無血清培養方法及び付着性動物細胞の無血清培養用培地 | |
| CN110564688B (zh) | 一种无血清培养角膜缘基质干细胞并于体外诱导成球和诱导分化的方法 | |
| US20050054099A1 (en) | Matrix derived from whole organ | |
| Zhou et al. | An improved method for directional differentiation and efficient production of neurons from embryonic stem cells in vitro | |
| CN118291372A (zh) | 一种皮肤成纤维细胞的非酶消化分离培养方法 |