JP2000103741A - Lipase inhibitor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a lipase inhibitor having high safety and capable of suppressing absorption of carbohydrates and preventing obesity, arteriosclerosis, or the like. SOLUTION: This lipase inhibitor is obtained by formulating solvent extracts of Gambir, a leaf of a guava (Psidium guajaba L.) and a leaf of a meadowsweet (Filipendula ulmaria). The method for extraction thereof may be carried out by extracting the Gambir and leaves with water or a hydrophilic organic solvent or a mixed liquid thereof. The resultant extracts can be formulated with a food and thereby readily ingested. The formulable food is not especially limited and the extracts can be formulated with a beverage, or the like, and applied to a diet food or a healthy food. The extracts can be utilized for general meals.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a food which is highly safe, suppresses fat absorption, and prevents obesity, heart disease, arteriosclerosis, and the like.

[0002]

2. Description of the Related Art In Japan, dietary habits have become westernized, the amount of fat intake increases year by year, the amount of exercise in social life decreases, the number of obese or obese people increases, and heart disease,
Atherosclerosis is also increasing. As a response, it is necessary to reduce fat intake and exercise moderately, but it is not easy to carry out. Therefore, it is necessary to cope with obesity, heart disease, arteriosclerosis, and the like by suppressing the absorption of ingested fat.

[0003] Asenyaku is Uncaria ga of the family Rubiaceae.
It is a dry water extract made by boiling mbir leaves and shoots with water, and is widely used as an astringent antidiarrheal or as an oral freshener.

[0004] Guava is called a bunjirou or a gemstone and is a plant belonging to the genus bunjiro in the family Myrtaceae, and its scientific name is Psidium guajaba L.
Generally, the berries are eaten raw, jam, juice, or the like. It is native to tropical America, but is widely cultivated in tropical and subtropical areas, cultivated in southern Kyushu in Japan, and wild in the Ryukyu Islands. Guava is also used as a drug in enteritis, dysentery, and indigestive diarrhea.
Leaves are considered a substitute for tea. Further, in Japanese Patent Application Laid-Open No. 5-246837 filed by the applicant of the present application, the use of a solvent extract of guava leaves as a cosmetic material is used to prevent whitening and rough skin.

[0005] Medousite has a scientific name of Filipendu
It is called la ulmaria, and its Japanese name is S. It is native to Europe and Asia, but is a natural perennial plant in North America and elsewhere.

[0006]

A lipase inhibitor which is safe and effective for obesity, heart disease and arteriosclerosis is obtained.

[0007]

As a result of diligent studies, it has been found that the problem can be solved by blending a solvent extract of Asenyaku, guava leaves, and medosweet leaves. These extraction methods may be performed using water, a hydrophilic organic solvent, or a mixture thereof.

[0008] If these are added to food, they can be easily taken. The food that can be blended is not particularly limited, and can be blended into beverages and the like and applied to diet foods and health foods. It can also be used for ordinary meals.

[0009]

EXAMPLES The present invention will be described more specifically with reference to the following production examples and examples. However, it goes without saying that the present invention is not limited to these production examples and examples.

[Example 1] 10 g of Asen medicine was added with 300 ml of a 50% aqueous ethanol solution and left for 5 days with occasional stirring. After filtration, evaporation and freeze-drying.

[Example 2] 10 g of guava leaves (dried product)
Was added to the mixture, and the mixture was left for 5 days with occasional stirring. After filtration, evaporation and freeze-drying.

[Example 3] To 10 g of leaves of meduus sweet (dried product), 300 ml of a 50% aqueous ethanol solution was added, and the mixture was left for 5 days with occasional stirring. After filtration, evaporation and freeze-drying.

[Prescription Example 1] Tablet (1) 200 g of extract of Example 1 (2) Lactose 500 g (3) Corn starch 290 g (4) Mg stearate 20 g (1), (2) and 170 g of corn starch are mixed. , Granulate with a paste made from 70 g of corn starch, add 50 g of corn starch to the granules and (4)
And the mixture is compressed on a press tablet machine to reduce the tablets to 100
0 tablets were manufactured.

Formulation Example 2 Beverage Extract of Example 2 1 g Xylitol 10 g Vitamin B ₁ hydrochloride 0.5 mg Vitamin B ₂ 0.2 mg Vitamin C 500 mg Niacin 1.0 mg Ca pantothenate 0.2 mg Weigh the above ingredients Mix and make up to 100 ml with purified water.

[Formulation Example 3] The extract prepared in Example 1 of Formulation Example 1 was changed to Example 3.

Lipase Inhibition Test Method 1.0 ml of a test product (0.5% aqueous solution of the example), 1.0 ml of a substrate solution, and 1.0 ml of an enzyme solution are weighed in a test tube and subjected to 40 ° C.
For 15 minutes. After 15 minutes, cold water 5.0
The temperature was returned to room temperature, and the absorbance at 425 nm was measured. Purified water was used in place of the test sample, and the inhibition rate of lipase inhibition was calculated as a control.

The method of preparing the reagents used in the above experiment was as follows. 1. Substrate solution 0.5 ml of pNP-Valerate solution is made up to 50 ml with substrate buffer. pNP-Valerate solution (Pentanoic acid 4-nitrophenyl
ester) Pentanoic acid 4-nitrophenyl ester (0.132 g) was made up to 10 ml with methanol. 3. Substrate buffer (0.05M-TrisBuffer) Tris (hydroxymethyl) aminomethane was added to 3.03.
g, 0.20 g of olefinsulfonic acid in purified water 500
After adjusting the volume to ml, adjust to pH 7.7 with hydrochloric acid. Enzyme solution Lipase (SIGMA; Type 2) 10 mg in dilution buffer 20 ml
Fixed volume. 5. Dilution buffer (0.05M-TrisBuffer) Tris (hydroxymethyl) aminomethane was added to 12.12.
g, olefin sulfonic acid 0.80 g, and calcium chloride monohydrate 0.32 g were made up to 2000 ml with purified water.

The results are shown in Table 1.

[Table 1]

Usage Test Eight adults were asked to eat one tablet of Formulation Example 1 or Formulation Example 3 at a time, and 100 g of Formulation Example 2 for three months at a time. In the comparative example, the extract of the production example was replaced with water from the example. (Comparative Examples 1 and 2) This was performed by 16 people in two groups. The following table summarizes the results of the questionnaire with the following criteria. Formulation example is very good 3 Formulation example is considerably better 2 Formulation example is slightly better 1 No difference 0 Comparative example is slightly better -1 Comparative example is much better -2 Comparative example Is much better -3

The experiment was performed as shown in Table 2.

[Table 2]

The results are shown in Table 3 below.

[Table 3]

[0022]

[Effect] A lipase inhibitor containing a solvent extract of asenyaku, guava leaf, and medosweet leaf is highly safe, suppresses carbohydrate absorption, and prevents obesity, heart disease, and arteriosclerosis.

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Claims (1)

[Claims] 1. A lipase inhibitor containing a solvent extract of asenyaku, guava leaf, and medosuit leaf.