JP2003342185A - Inhibitor of lipase activity - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a naturally originating inhibitor of lipase activity strongly inhibiting the lipase activity and effectively used for preventing and treating fatness and diseases caused by fatness as a risk factor and provide a composition for industrially and effectively using the same. <P>SOLUTION: The inhibitor of lipase activity contains a dry powder, an extract or a purified product of roots and/or rhizomes of a plant belonging to the genus Astilbe, preferably Astilbe thunbergii, more preferably a purified product which is the extract with ethanol, acetone or a water-containing solvent of those or an ethyl acetate soluble fraction of the extract with water-containing acetone. More preferably, the inhibitor is prepared by using a polyphenol together with the purified product. A food composition or a pharmaceutical composition is prepared by adding the inhibitor. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a lipase activity inhibitor using a specific plant as a raw material and its use. More specifically, the present invention relates to a lipase activity inhibitor containing a plant belonging to the genus Tedalia genus such as red pepper and an edible or pharmaceutical composition containing the same.

[0002]

2. Description of the Related Art In recent years, in Japan, the number of western-style diets has increased, overeating and energy intake have increased, and the tendency for obesity to increase has been increasing in combination with chronic lack of exercise. Obesity is gaining attention as a risk factor for lifestyle-related diseases such as high blood pressure, heart disease, and diabetes. In order to treat obesity, there are generally diet therapy that limits calorie intake and exercise therapy that aims at energy consumption, but it requires a long-term continuation and is accompanied by a mental load. The treatment by administration is mainly applied to severely obese patients, and there is a fear of side effects of the drug, and thus it is difficult to say that they are general-purpose methods.

From the viewpoint of preventing obesity, substances that inhibit the activity of digestive enzymes such as amylase and lipase have been developed in order to suppress excessive intake energy. Lipase is a digestive enzyme for lipids, which is secreted from the pancreas in the body and acts on triglyceride which is a major component of fat to hydrolyze ester bonds to produce diglyceride, monoglyceride, fatty acid and glycerin, which are absorbed from the small intestine. Usually becomes an energy source. However, in the case of low calorie consumption due to lack of exercise or excessive fat intake, the absorbed fatty acids are not used for energy consumption, and are resynthesized in the body and accumulated in tissues,
Eventually, he becomes obese. Therefore, a substance that inhibits lipase activity is useful for the prevention and treatment of obesity and the diseases associated therewith.

Various lipase activity inhibitors have been proposed so far, for example, epigallocatechin gallate (JP-A-3-228664), which is a component of green tea, bell pepper, pumpkin, shimeji mushroom, hijiki, green tea, Water extract such as oolong tea (JP-A-3-219872), hinokitiol (JP-A-8-268882), tamarind seed coat extract containing procyanidin as an active ingredient (JP-A-9-291039), defatted rice bran Hot water extract (Japanese Patent Laid-Open No. 2001-97880), polyphenol-based substances contained in hops (Japanese Patent Laid-Open No. 2001-3
21166).

[0005]

However, although the lipase activity inhibitors proposed so far have excellent effects when used as medicines, there are side effects, and the dosage should be adjusted under the guidance of a doctor. There were restrictions such as what we had to protect. In addition, the extracts and ingredients separated from the food ingredients have a low effect in practice, are based on the results of experiments under unpractical intake conditions, or must be ingested in large amounts in a normal dietary form. However, none of them had a sufficiently satisfactory effect.

In view of the above situation, the present invention provides a lipase activity inhibitor derived from a natural product, which strongly inhibits lipase activity and can be effectively used for the prevention and treatment of obesity and various diseases in which it is a risk factor. Another object was to provide a composition that can be effectively utilized in industry.

[0007]

[Means for Solving the Problems] In order to solve the above problems, the present inventors have conducted extensive studies on the relationship between a large number of plant raw materials and their extracts and lipase activity inhibitory action. The present inventors have found that the first plants belonging to the genus Tedalia genus exert the desired effects of the present invention remarkably, and have completed the present invention.

[0008] That is, according to the present invention, there is provided a lipase activity inhibitor comprising a plant belonging to the genus Tedalia. There is also provided a lipase activity inhibitor, which comprises a plant belonging to the genus Tedalia and polyphenols. Further, in the present invention,
There is provided an edible composition or a pharmaceutical composition containing these lipase activity inhibitors.

[0009] Here, the plant belonging to the genus Tedalia is a plant of the genus Astilbe of the family Asteraceae, and a representative example thereof is red pepper, which is a dry powder or extract of its roots and / or rhizomes (hereinafter referred to as an extract). It is) or a purified product is preferably used. It is desirable that the extract of the plant belonging to the genus Tedalia is extracted with water or a hydrophilic organic solvent, especially with ethanol, acetone or a hydrate thereof, and further an extract using hydrated acetone. It is more desirable that the fraction is soluble in ethyl acetate.

The polyphenols applied to the lipase activity inhibitor of the present invention are one or more selected from the group consisting of proanthocyanidins, tannins, catechins, flavones, flavonols, isoflavones and glycosides thereof. Desirably, the embodiment is one or more dry powders selected from the group consisting of grape seeds, guava leaves, immature apples or skins of apples, Tochu leaves, Pu'er tea leaves, Akamega wrinkle bark, green tea leaves and cocoa beans, Extracts or purified products are more preferred.

The edible composition and the pharmaceutical composition of the present invention are characterized by containing the above lipase activity inhibitor.

[0012]

BEST MODE FOR CARRYING OUT THE INVENTION First, the lipase activity inhibitor of the present invention is characterized by containing a plant belonging to the genus Tedalia. Ted Keshi (Astilbe)
Plants belonging to the genus are classified into the family Yukinoshita, and there are various examples of the one according to the present invention as described later, but as a representative example, a red capsicum (scientific name: Astilbe thunber
gii (SIEB. et ZUCC.) MIQ. ) Can be mentioned. Red rhizoma is a perennial plant that grows naturally in the mountains of Japan. Its rhizome is called red sardine and has been used since ancient times for the purpose of lowering fever, detoxification, extinction, and so on (Cicicifuga simplex from the family Ranunculaceae).
WORMSKORD, etc.) has been used as a substitute. In the present invention, what is referred to as red or red soybean is also included.

Astil as an example of the plant of the genus Astilt
be chinensis, Astilbe aust
rosinensis, Astilbe thunbe
rgii, Astilbe thumbergii (S
IEB. et ZUCC. ) Miq. : Akasho,
Astilbe thumbergii (SIEB.e
t ZUCC. ) MIQ. var. congesta
BOISS. (= Astilbe odontophy
lla MIQ. ): Triassium, Astilb
e polyandra, Astilbe grand
is, Astilbe rivularis, Asti
lbe Japanica (MORR. et DECN
E. ) A. GRAY: Hawthorn Shouma, Astilb
e microphylla KNOLL: Tweezer, Astilbe myrantha, and the like.

The embodiment of the plant belonging to the genus Tedalia such as red pepper and the like used in the present invention is preferably the root and / or rhizome of the plant, and the root and / or rhizome itself, which is subjected to a treatment such as drying, shredding or crushing. What has been applied, an extract obtained by extracting these with a solvent, an extract obtained by removing the solvent from the extract, and an adsorbent such as silica gel, magnesium silicate, ion exchange resin, activated alumina, cellulose, activated carbon, etc. in the extract. Any of the purified products that have been subjected to purification treatment such as column chromatography and solvent fractionation used may be used. When used for food applications, it is convenient to use a powder obtained by drying and appropriately crushing the roots and / or rhizomes of the plant, or an extract obtained by extracting fine fragments or powder of the dried product with water or a hydrophilic organic solvent. It is desirable from the standpoint of productivity and manufacturing cost. When used for pharmaceutical purposes, the above-mentioned extract, extract or highly purified product is desirable.

As a hydrophilic organic solvent, lower monohydric alcohols such as methanol, ethanol, propanol and isopropanol, polyhydric alcohols such as propylene glycol, 1,3-butanediol and glycerin, acetone,
Examples thereof include methyl ethyl ketone, ether, petroleum ether, ethyl acetate, and hydrates and mixtures thereof. In order to efficiently obtain the extract for achieving the desired effect of the present invention, it is preferable to use ethanol, acetone, ethyl acetate and a water-containing substance thereof as the extraction solvent. The water content of the hydrated substance is, for example, 1 to 9 in the case of ethanol.
9% by weight, more preferably 10 to 50% by weight, in the case of acetone 1 to 50% by weight, more preferably 10% by weight.
-30% by weight, and in the case of ethyl acetate, it is 80-99% by weight, more preferably 85-95% by weight. Outside these ranges, the desired effects of the invention are diminished or the yield of extract is reduced.

In order to obtain the extract according to the present invention simply and efficiently, it is preferable to extract with water-containing ethanol or water-containing acetone and further fractionate the extract with ethyl acetate to collect the soluble fraction. The extraction treatment is 1 to 1 for the raw material to be treated.
About 100 times by weight of the extraction solvent is added, and the mixture is appropriately stirred under normal pressure or pressure at room temperature or under heating for 10 minutes to
Extract for a few days. The insoluble matter can be removed by filtration or centrifugation to obtain the extract according to the present invention, and the solvent can be removed from the extract by a known means such as distillation under reduced pressure, spray drying, and freeze drying. Such an extract can be obtained.

It is known that, in addition to starch and tannin, flavonoids such as bergenin, astilbin, and astilbic acid are contained in the roots and rhizomes of plants belonging to the genus Rhinoceros such as red pepper. It is said to exhibit the above-mentioned pharmacological action (Shimad
a, H.A. Et al., Yakugaku Zasshi, 72nd.
Vol. Pp. 578-588, 1952). On the other hand, the present invention has found that the above-mentioned forms of plants of the genus Tedalia genus such as red pepper significantly inhibit the activity of lipase.

The polyphenols used in the lipase activity inhibitor of the present invention may be known ones, but plant polyphenols are easily available and simple. This includes hydrolyzed tannins, catechins, condensed tannins (such as proanthocyanidins), flavones, flavonols, isoflavones and the like. Examples of hydrolyzable tannins include gallotannins (quintanal tannins, gallic tannins, etc.), ellagitannins (pedencragin, telimagladine, cajualinins, etc.), ellagitannin oligomers (agrimoninin, enotein B, etc.), cafetannins (rosmarinic acid, lithospermic acid, etc.). it can. Catechin is epicatechin, epigallocatechin, epigallocatechin gallate, etc., condensed tannin is a catechin condensate, and examples of proanthocyanidins such as propelargonidin, procyanidin, and prodelphinidin and their acid decomposition products (anthocyanidins) Can be mentioned as. Examples of the flavone include apigenin, luteolin, flavonols such as quercetin, quercitrin, isoquercitrin, rutin, kaempferol, and the like, and isoflavones such as daidzein, genistein, isogenin, and glycosides thereof.

In the present invention, hydrolyzable tannins, catechins, proanthocyanidins, flavonols, isoflavones and glycosides thereof, more preferably hydrolyzable tannins, catechins and flavonols, alone or in combination of two or more, It is used as a lipase activity inhibitor together with the above-mentioned plants of the genus Tedalia.

The polyphenols used in the present invention may be chemically synthesized pure products, but natural products containing them, more preferably plants, are used as raw materials in accordance with known methods for drying, crushing, extraction, concentration, It is preferable to use a dry powder, an extract and a purified product obtained by performing a treatment such as distillation. That is, although it is not particularly limited as long as it is a plant containing the polyphenols, it is preferably grape seed, guava leaf, unripe apple or skin of apple, Tochu leaf, Pu'er tea leaf, Akamega wrinkle bark, green tea leaf, mulberry leaf, buckwheat. From the whole grass, soybean, French coastal pine, cacao bean, etc., one or more kinds can be used as raw materials in the following forms.

The form is such that the above-mentioned plants are subjected to processing such as drying, shredding or crushing, an extract obtained by adding a solvent to them, an extract obtained by removing the solvent from the extract, Silica gel, magnesium silicate,
It may be any purified product that has been subjected to a purification treatment such as column chromatography or solvent fractionation packed with an adsorbent such as an ion exchange resin, activated alumina, cellulose or activated carbon. In order to achieve the desired effects of the present invention, when used for food applications, dry powder, an extract extracted with water and / or a hydrophilic organic solvent is convenient and convenient, and is used for pharmaceutical applications. If so, the above-mentioned extract or purified product is desirable.

The extraction treatment includes lower monohydric alcohols such as methanol, ethanol, propanol and isopropanol, propylene glycol, 1,3-butanediol,
A polyhydric alcohol such as glycerin, a hydrophilic organic solvent such as acetone, methyl ethyl ketone and a mixture thereof or a water-containing solvent thereof is used, and the extraction solvent is added in an equal weight to about 100 weight times with respect to the raw material. An extraction liquid containing polyphenols can be obtained by removing the insoluble matter by centrifugation or filtration by subjecting to an extraction treatment for 10 minutes to several days while appropriately stirring under pressure or pressure at room temperature or under heating, and further performing the extraction. The polyphenol-containing extract can be prepared by removing the solvent from the liquid by means of distillation under reduced pressure, freeze-drying, spray-drying and the like. In addition, you may utilize a commercial item as these.

A preferred embodiment of the lipase activity inhibitor of the present invention is obtained by using the plant and the roots and / or rhizomes of the plant belonging to the genus Tedalia, such as the red rhizome, the dried product, the extracted product or the purified product as an essential raw material, obtained as described above. It is to be contained. A more desirable embodiment is an essential main raw material derived from a plant of the genus Weeder, which contains polyphenols in combination therewith, and most preferably uses a rhizome extract or a purified product of red rhizome as a main raw material. Yes, and grape seeds, guava leaves, unripe apples or skins, Tochu leaves, Pu'er tea leaves,
One or more dry powders, extracts or purified products selected from the group consisting of red bark wrinkle bark, green tea leaves and cocoa beans are used in combination.

When the raw material derived from the plant of the genus Tedalia and the raw material containing polyphenols are used in combination, the lipase activity inhibiting effect is more remarkably exhibited. The mixing ratio of both raw materials in the lipase activity inhibitor can be appropriately changed depending on the purpose of use and application for producing a desired effect, production cost, etc., and is a plant-derived raw material of the genus Weeder / polyphenol-containing raw material = 100. / 0 to 0/100 (weight ratio; the same applies hereinafter unless otherwise specified), more preferably 100/0 to 50/50, and most preferably 90.
/ 10 to 60/40.

In the lipase activity inhibitor of the present invention, various raw materials and components can be used in combination unless they are against the gist of the present invention. For example, excipients used in ordinary foods and pharmaceuticals, moisture-proofing agents, preservatives, strengthening agents, thickeners, emulsifiers, antioxidants, sweeteners, acidulants, seasonings, colorants,
There are fragrances. Further, it is also one of the preferable embodiments of the present invention to use a known material having a lipase activity suppressing effect in combination with a material other than the above.

As described above, according to the present invention, a dried product, an extract or a purified product of a plant belonging to the genus Ternaceae such as red capsicum, or a dried product, an extract or a product containing polyphenols is contained. And a lipase activity inhibitor comprising This lipase activity inhibitor is excellent in the effect of inhibiting lipase activity, reduces the absorption of lipids in the body by suppressing the decomposition of dietary fat, promotes the metabolism of blood lipid metabolism, and is associated with obesity and this If possible, prevent various diseases. Such a lipase activity inhibitor may be used as it is for the desired purpose of the present invention, but the present invention also provides a composition comprising the same. Aspects of the composition include food and drink, food additives,
There are various kinds of livestock feed, pet food, etc., and an edible composition or a medicinal composition is preferable.

The edible composition of the present invention is characterized by containing the above-mentioned lipase activity inhibitor. Said lipase activity inhibitor is a plant belonging to the genus Tedalia such as red pepper and the like, which is preferably a dry powder, an extract or a purified product of roots and / or rhizomes as an essential raw material. Alternatively, it comprises a dry powder of plant, an extract or a purified product containing the essential raw material and polyphenols.

As an embodiment of this edible composition, the dry powder, the extract or the purified product as it is or the lipase activity inhibitor as a liquid, gel, powder or solid food, for example, fruit drink, refreshing Beverages, teas, soups, jellies, yogurts, puddings, cake mixes, sprinkles, miso, soy sauce, dressings, mayonnaise, seasonings such as grilled meat sauce, noodles, processed meat and meat products such as ham and sausages, jam, milk, cream , Powdered products such as butter and cheese, solid or liquid dairy products, margarine, bread,
It can be added to cakes, cookies and the like.

If necessary, dextrin, lactose,
Starch or its processed materials, excipients such as cellulose powder, vitamins, minerals, oils and fats of animals and plants and seafood, proteins, sugars, pigments, flavors, and other edible additives, powders, granules, pellets, tablets It can be used as a dietary supplement or health food by processing it into a capsule or the like, or forming a capsule by coating with gelatin or the like. At this time, a composition in which a known edible material having the above lipase activity inhibitory action is used in combination is preferable. In addition, the edible composition of the present invention covers a wide variety of forms and is not limited to the above examples, but contains a large amount of fats and sugars from the viewpoint of suppressing in vivo absorption of dietary fat and improving lipid metabolism. It is desirable to add it to the foods contained in 1) or process it into the form of the above-mentioned dietary supplements and health foods.

The amount of the lipase activity inhibitor of the present invention to be mixed in the above foods and edible compositions is uniformly varied depending on the type of the edible composition, the shape of the bear, the purpose of use and the type and form of the lipase activity inhibitor. Although it is difficult to specify, when it is added to general processed foods, for example, the lipase activity inhibitor of the present invention is an extract obtained by extracting rhizome of red rhizome with water-containing ethanol having a water content of 50% by weight: guava leaf having a water content of 50. If the extract is 50:50 (weight ratio) extracted with wt% water-containing ethanol, the amount is generally 0.01 to 50 wt%, and more preferably 0.1 to 30 wt%. If the amount is out of this range, the desired effect of the present invention by oral ingestion is small, and if the amount is too large, the flavor may be impaired depending on the type of food or it may be impossible to prepare the food. The lipase activity inhibitor of the present invention may be used as it is for food.

The pharmaceutical composition of the present invention may be prepared by adding a known excipient or additive to the above-mentioned lipase activity inhibitor as required unless it is contrary to the gist of the present invention, and processing the tablets by a conventional method to give tablets. The preparations are capsules, granules, powders and the like. It is mainly administered orally to suppress absorption of ingested fat in the body, and is applied for improvement and treatment of blood lipid balance, and prevention or treatment of various diseases such as obesity and accompanying hyperlipidemia. It is difficult to uniformly set the amount of the lipase activity inhibitor of the present invention to be mixed depending on the form, the type of the pharmaceutical preparation, the form, the usage, the dose, etc., but it is generally 0.01 to 50% by weight. The amount of intake when orally administered is not particularly limited. For example, the extract obtained by extracting the rhizome of Rhododendron rhizome with water-containing acetone having a water content of 20% by weight can be further fractionated with ethyl acetate. Soluble fraction: An extract of grape seeds extracted with ethanol = 75:25 (weight ratio) based on an adult (body weight 50K
g) 0.01 to 20 g per day, more preferably 0.
It is 1 to 10 g. When the amount is out of this range and the amount is small, the desired effect is lowered, and when the amount is too large, further effect cannot be expected.

[0032]

EXAMPLES Example 1 Red-tailed ginger (Astilbe thumbergii)
(SIEB. Et ZUCC.) MIQ. The dried rhizome of (1) was crushed to a size of 3 to 5 mm square, and this was further processed by a crusher to prepare a fine powder (Sample A-1) which passed a 200 Tyler mesh.

Example 2 1 kg of crushed pieces of red rhizome rhizome obtained in Example 1 was placed in a stainless steel extraction kettle, 10 L of hydrous ethanol having a water content of 45% by weight was added, and the mixture was stirred at 70 ° C. for 6 hours.
It was time-extracted. Then, the residue was filtered off to obtain an extract, and the solvent was distilled off from the extract under reduced pressure to obtain 85 g of a reddish brown extract (Sample A-2).

Example 3 1 kg of fine powder of Rhizoma rhizome obtained in Example 1 was placed in a stainless steel extraction pot, 5 L of water-containing acetone having a water content of 30% by weight was added, and the mixture was extracted under reflux for 3 hours and then filtered. It was separated into an extract and a residue. 5 L of the above-mentioned water-containing acetone was added to the residue again and treated in the same manner to obtain an extract. Both extracts were combined and the solvent was distilled off under reduced pressure to prepare 72 g of a reddish brown extract (Sample A-3).

Example 4 Rhizoma rhizome extract obtained in Example 3 (Sample A-
3) 50 g was suspended in 1 L of distilled water, and the suspension was subjected to a solvent fractionation treatment by dividing it into 200 mL portions of ethyl acetate 5 times each. The solvent was distilled off under reduced pressure to obtain an ethyl acetate-soluble fraction as a purified product. 33 g of (Sample A-4) was prepared.

Example 5 Astilbe odontoph
ylla MIQ. After drying the rhizome of (1) in the sun and drying it, 1 Kg of this crushed piece was treated in the same manner as in Example 2 to obtain 83 g of a reddish brown extract (Sample A-5).

Comparative Example 1 A dried product obtained by sun-drying fresh leaves of guava was crushed to a size of about 5 mm square or less, and further pulverized to prepare a fine powder (sample C-1) passing through a 200 Tyler mesh. .

Comparative Example 2 1 kg of crushed pieces of dried guava leaf obtained in Comparative Example 1 was charged into a stainless steel extraction kettle and water / ethanol (weight ratio: 1
/ 1) After adding 20 L of mixed solvent and performing an extraction treatment under reflux for 5 hours, the residue was filtered off to obtain an extract, and the solvent was distilled off from the extract under reduced pressure to extract a brown guava leaf-derived tannin-containing extract. 380 g of the product (Sample C-2) was obtained.

Comparative Example 3 A dried product of grape seeds was crushed to a size of about 2 mm square or less, 1 kg of this was charged into a stainless steel extraction kettle, and 10 L of hydrous ethanol having an ethanol concentration of 75% by weight was added to the mixture under reflux. After the time extraction treatment, the residue was filtered off to obtain an extract, and the solvent was distilled off from the extract under reduced pressure to obtain a brown grape seed-derived proanthocyanidin-containing extract (Sample C
-3) 280 g was prepared.

Test Example 1 With respect to various processed products of red pepper and polyphenol-containing substances according to the present invention, the influence on the lipase activity was examined by the method described below. That is, oleic acid triglyceride 8 purchased from Wako Pure Chemical Industries, Ltd.
Tris buffer containing 0 mg, phosphatidylcholine 10 mg, taurocholic acid 5 mg and sodium chloride 0.1 mol / L (N-tris (hydroxymethyl) methyl-2
-Aminoethanesulfonic acid, pH 7.0) 9 mL was sonicated for 5 minutes, and 0.1 mL of pancreatic lipase 0.0
5 mL (10 units) and 0.1 mL of test substance solution (dissolved or dispersed in the buffer solution) of a predetermined concentration
Was added and incubated at 37 ° C. for 30 minutes.

Then, 250 μL of this incubation solution
In a test tube and add 2% methanol in chloroform / n
-Add 3 mL of the heptane (1/1) mixture and add 1 on a shaker.
After horizontal shaking for 0 minutes for extraction, centrifugation was performed for 5 minutes (2000 × g), and the upper layer was removed by suction. Further, 1 mL of copper reagent was added to the lower layer, shaken for 10 minutes, and then centrifuged for 10 minutes (2000 × g).

Next, 0.5 mL of the upper organic layer containing the extracted copper salt of fatty acid was added to 0.1% of chloroform containing 0.05% of 3- (2) -t-butyl-4-hydroxyanisole. Treated with 0.5 mL of metal chelating agent (basocuproine: 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline) at a weight / volume% concentration,
Absorbance at 480 nm was measured with a spectrophotometer (ORD / UV-5 spectrometer, manufactured by JASCO Corporation). The amount of free fatty acid produced by this was determined.

The pancreatic lipase activity, that is, the lipolytic ability by the lipase, is determined as the number of moles of oleic acid produced per hour from 1 L of the reaction mixture which is the above-mentioned incubation solution, and the lipase activity in the presence of each test substance is It was shown as a relative value (compared with the average value of n = 6) when the lipase activity in the control test in which the test substance was not added was 100. The results are shown in Table 1.

[0044]

[Table 1]

From the data in Table 1, the lipolytic ability by pancreatic lipase is remarkably suppressed depending on the concentration added of the test substances (samples A-1 to A-5) derived from plants belonging to the genus Tedalia such as red pepper and the like. It was revealed that the processed products of known materials (Samples C-1 to C-3) had a small effect of suppressing the lipolysis. Furthermore, it was found that the combined use of the processed product derived from a plant belonging to the genus Pleurotus such as red pepper and the aforementioned processed product of a known polyphenol-containing material synergistically suppresses lipolytic activity by pancreatic lipase. .

Example 6 Mixture of Sample A-2 and Sample C-3 (1: 1, weight ratio)
A lipase activity inhibitor of the present invention consisting of 120 mg, Gymnema sylvestre leaf extract 30 mg, and beeswax 40 mg
Then, 60 mg of borage oil was added, and the mixture was thoroughly mixed at about 45 ° C. to be in a homogeneous state, which was then subjected to a capsule filling machine to manufacture a gelatin-coated capsule preparation having an internal content of 250 mg per grain. A monitoring test was conducted on 10 volunteers (adults and men) of this capsule formulation, who took one capsule of the above-mentioned formulation at meal time and three capsules per day for two weeks while maintaining a normal diet form. Weight loss was noted. This preparation can be used as an orally ingestible edible composition or medicinal composition for preventing obesity.

Example 7 Sample A-2: 5.0 kg of the lipase activity inhibitor of the present invention consisting of oolong tea leaf powder = 3: 1 (weight ratio) was used as a modified starch (Matsuya Chemical Co., Ltd., trade name: Pine Flow). ) 3.5K
g, tricalcium phosphate 0.3 kg, vitamin B
₁ 0.3Kg, Vitamin B ₂ 0.2Kg, Vitamin B ₆
The mixture was placed in a compounding machine together with 0.2 kg and vitamin C 0.5 kg, and mixed with stirring for 10 minutes. The mixture was supplied to a direct compression type tableting machine to prepare a tablet having a diameter of 7 mm, a height of 4 mm and a weight of 150 mg, and the shellac thin film was coated with a coating machine to produce a tablet-shaped food product.

Example 8 Butter 110g and shortening 1 in household whipper
Put 10 g, 90 g of white sucrose and 100 mL of milk, add 1 chicken egg with stirring and mix well, then soft flour 1
90 g, 2 g of baking powder and 10 g of the lipase activity inhibitor of the present invention consisting of sample A-1 were added and kneaded sufficiently. After letting it rest for 30 minutes, it was divided into 50 pieces with a mold and baked in an oven to make a trial butter cookie.

Example 9 1 L of commercially available orange juice was mixed with Sample A-2: grape seed extract (trade name: Activin, manufactured by Inter Health).
= 2: 1 (weight ratio) of the lipase activity inhibitor of the present invention (5 g) was added and mixed to prepare an obesity-preventing orange juice. This was no better than the original orange juice.

[0050]

Industrial Applicability According to the present invention, there is provided a lipase activity inhibitor comprising a plant belonging to the genus Tedalia.
This lipase activity inhibitor is a dry powder of roots and / or rhizomes of plants belonging to the genus Rhododendron such as red salamander and the like, and is caused by processed products such as an extract or a purified product thereof, which significantly enhances the ability of the pancreatic lipase to hydrolyze fat. Has the effect of suppressing Further, in addition to the processed powders of the roots and / or rhizomes of the plants belonging to the genus Pleurotus such as red pepper, the extract or the purified product thereof, the dry powder of the plant containing polyphenols, the extract or the purified product thereof. There is provided a lipase activity inhibitor comprising a processed product such as a product, which synergistically exhibits a remarkable lipase activity inhibitory effect. Furthermore,
According to the present invention, there is provided an edible composition or a pharmaceutical composition containing the lipase activity inhibitor, wherein the composition suppresses intestinal absorption of fat by a high-fat diet, thus suppressing intake energy. It is useful for preventing weight gain, improving blood lipid balance, and preventing or treating various diseases related thereto.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61P 43/00 111 A61P 43/00 111 C12N 9/99 C12N 9/99 F term (reference) 4B018 MD07 MD48 MD52 MD57 MD59 MD60 ME01 MF01 4C084 AA19 MA02 MA52 NA14 ZA701 ZA702 ZC202 4C088 AB66 AC11 AC12 BA08 BA10 CA06 CA07 CA11 MA52 NA14 ZA70 ZC20

Claims (9)

[Claims] 1. A lipase activity inhibitor comprising a plant belonging to the genus Tedalia. 2. The lipase activity inhibitor according to claim 1, wherein the plant belonging to the genus Pleurotus cornucopia is a red rhizome, and a dry powder, extract or purified product of its root and / or rhizome is used. 3. The lipase activity inhibitor according to claim 1 or 2, wherein the extract of the plant belonging to the genus Tedalia genus is extracted using water or a hydrophilic organic solvent. 4. The lipase activity inhibitor according to claim 3, wherein the hydrophilic organic solvent is ethanol, acetone or a hydrate thereof. 5. A purified product of a plant belonging to the genus Tedalia genus is an ethyl acetate-soluble fraction of a hydrous acetone extract.
4. The lipase activity inhibitor according to any one of 4 to 4. 6. A lipase activity inhibitor, wherein the lipase activity inhibitor according to any one of claims 1 to 5 further contains polyphenols. 7. The lipase activity inhibitor according to claim 6, wherein the polyphenols are one or more selected from the group consisting of proanthocyanidins, tannins, catechins, flavones, flavonols, isoflavones and glycosides thereof. 8. A dry powder of one or more polyphenols selected from the group consisting of grape seeds, guava leaves, immature apples or skins of apple, Tochu leaf, Pu'er tea leaves, Akamega wrinkle bark, green tea leaves and cocoa beans. 8. The lipase activity inhibitor according to claim 6 or 7, which is an extract or a purified product. 9. An edible composition or a pharmaceutical composition comprising the lipase activity inhibitor according to any one of claims 1 to 8.