JP2000102362A - Food additive and food composition containing the same - Google Patents
Food additive and food composition containing the sameInfo
- Publication number
- JP2000102362A JP2000102362A JP10293038A JP29303898A JP2000102362A JP 2000102362 A JP2000102362 A JP 2000102362A JP 10293038 A JP10293038 A JP 10293038A JP 29303898 A JP29303898 A JP 29303898A JP 2000102362 A JP2000102362 A JP 2000102362A
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- Japan
- Prior art keywords
- food
- hyaluronic acid
- food additive
- present
- molecular weight
- Prior art date
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、食品に関する技術
分野の発明である。より具体的には、主に機能性食品に
おける添加物及びこの添加物を含有する食品組成物に関
する発明である。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a technical field relating to food. More specifically, the invention mainly relates to an additive in a functional food and a food composition containing the additive.
【０００２】[0002]
【従来の技術】近年の健康ブームにより、様々な体調調
整機能を有する特定保健用食品、特別用途食品等（いわ
ゆる機能性食品）が提供されている。かかる食品におけ
る体調調整機能には、ダイエット機能や便秘解消機能等
の、使用者の健康と共に美容に大きく係わる機能が数多
く認められる。このような機能性食品の体調調整機能の
うち、さらに美容に深く係わる機能が付加されている機
能性食品も提供されつつある。2. Description of the Related Art Due to the recent health boom, foods for specific health use, foods for special use and the like (so-called functional foods) having various physical condition adjusting functions have been provided. As the physical condition adjusting function of such foods, there are many functions that greatly affect the health and beauty of the user, such as a diet function and a constipation relieving function. Among such physical condition adjusting functions of functional foods, functional foods to which a function deeply related to beauty is added are being provided.
【０００３】[0003]
【発明が解決すべき課題】機能性食品を開発するにあた
って、最も重要なことは、食用に耐え得る程に安全性に
優れ、かつ、優れた効果を有する食用成分を見出すこと
であることは言うまでもない。In developing a functional food, it is needless to say that the most important thing is to find an edible component which is safe enough to be edible and has an excellent effect. No.
【０００４】本発明において解決すべき課題は、より美
容効果に優れた機能性食品を提供し得る鍵となる食用成
分を見出し、この食用成分が食品添加物として添加され
た、体調調整機能を伴うことは勿論のこと、特に美容効
果において優れる機能性食品として好適な食品を提供す
ることにある。[0004] The problem to be solved in the present invention is to find a key edible component that can provide a functional food with more excellent cosmetic effects, and to add a edible component as a food additive, which has a physical condition adjusting function. Needless to say, it is to provide a food suitable as a functional food which is particularly excellent in beauty effects.
【０００５】[0005]
【課題を解決するための手段】本発明者は、この特に美
容効果に優れた機能性食品として好適な食品を提供する
ための鍵となる成分を見出すために、鋭意検討を重ね
た。その結果、現在、医薬の分野や化粧品の分野で用い
られている酸性ムコ多糖（グリコサミノグリカン）の一
種であるヒアルロン酸のうち、特に低分子のものを選択
して食品の成分とすることにより、美肌や便秘解消等と
して現れる体調調整機能や美容効果に優れる、機能性食
品として好適な食品が提供され得ることを見出し、本発
明を完成した。Means for Solving the Problems The present inventors have conducted intensive studies in order to find a key component for providing a food suitable as a functional food having particularly excellent cosmetic effects. As a result, of hyaluronic acid, a kind of acidic mucopolysaccharide (glycosaminoglycan) currently used in the fields of medicine and cosmetics, select especially low-molecular-weight hyaluronic acid as a food ingredient As a result, the present inventors have found that a food suitable as a functional food, which is excellent in a physical condition adjusting function and a beauty effect which appear as beautiful skin and constipation resolution, etc., can be provided, and completed the present invention.
【０００６】ヒアルロン酸には、その物理的・生理的作
用として、(a) 細胞外液の電解質と水分の調節作用，
(b)瘢痕形成抑制作用，(c) 潤滑作用，(d) 保水作用，
(e) 粘弾性等が認められ、これらの作用に着目した、ヒ
アルロン酸を有効成分とする整形外科や眼科領域におけ
る医薬品や、ヒアルロン酸を配合した化粧料が、既に提
供されている。Hyaluronic acid has the following physical and physiological actions: (a) the action of regulating the electrolyte and water content of extracellular fluid;
(b) Scar formation inhibitory action, (c) Lubrication action, (d) Water retention action,
(e) Viscoelasticity and the like have been recognized, and pharmaceuticals in the field of orthopedics and ophthalmology using hyaluronic acid as an active ingredient and cosmetics containing hyaluronic acid have already been provided, focusing on these effects.
【０００７】すなわち、皮膚や関節の老化または病的状
態により細胞機能が低下してくると、生体ヒアルロン酸
量は減少し、その結果、皮膚の乾燥、肌荒れ、ハリ、弾
力性の減少、シミ、シワの増加、あるいは関節の湿潤性
悪化による関節痛等を引き起こす。そして、このような
状態を改善すべく、皮膚にヒアルロン酸や自然保湿因子
等の生体成分を配合した化粧料を塗布したり、関節に直
接ヒアルロン酸を注入する等の方法がとられている。[0007] That is, when cell function is reduced due to aging or pathological condition of the skin or joints, the amount of biological hyaluronic acid is reduced, and as a result, skin dryness, rough skin, firmness, reduced elasticity, stains, It causes an increase in wrinkles or joint pain due to deterioration of the wettability of the joint. In order to improve such a condition, a method of applying a cosmetic containing a biological component such as hyaluronic acid or a natural moisturizing factor to the skin, or injecting hyaluronic acid directly into a joint has been adopted.
【０００８】本発明者は、このような優れた作用を有す
るヒアルロン酸を、食品添加物として用いることによ
り、体調調整機能だけではなく、特に、美肌作用等が認
められる美容目的を達成し得る機能性食品として好適な
食品を提供し得ることに想到した。The inventor of the present invention has found that by using hyaluronic acid having such an excellent action as a food additive, it is possible to achieve not only a physical condition-adjusting function but also, in particular, a function capable of achieving a cosmetic purpose in which a beautiful skin effect is recognized. It has been conceived that a food suitable as a sex food can be provided.
【０００９】しかしながら、従来の医薬品や化粧品等の
用途において用いられているヒアルロン酸は高分子量で
あるため、経口摂取しても、腸管吸収させることが困難
であると考えられる。つまり、従来から上記の分野にお
いて用いられているヒアルロン酸を、そのまま食品添加
物として用いても、これを添加した機能性食品に、生体
内部からの根本的な改善効果、例えば、体調調整機能や
美肌効果等を期待することができなかった。However, since hyaluronic acid used in conventional applications such as pharmaceuticals and cosmetics has a high molecular weight, it is considered that it is difficult to absorb it in the intestinal tract even when taken orally. In other words, hyaluronic acid conventionally used in the above fields, even if used as a food additive as it is, the functional food to which it is added, a fundamental improvement effect from the inside of the living body, for example, Could not expect a beautiful skin effect.
【００１０】また、従来から上記の分野において用いら
れているヒアルロン酸は、医薬品や化粧品に配合するこ
とを目的として製造されているために、高度に精製され
ており、食品添加物として用いるには高価に過ぎること
は否めない。Further, hyaluronic acid, which has been conventionally used in the above-mentioned fields, has been produced for the purpose of blending into pharmaceuticals and cosmetics, and is therefore highly purified. It cannot be denied that it is too expensive.
【００１１】そこで、本発明者は、腸管吸収性が向上し
たヒアルロン酸の供給源として、例えば、食用に供さ
れ、かつ、入手が比較的容易なニワトリ等の体の一部で
ある「鶏冠」を選択し、さらに、この中に含まれるヒア
ルロン酸を低分子化する手段を施し、これを食品添加物
とすることにより、上記の課題を解決することを見出し
た。Accordingly, the present inventor has proposed, as a source of hyaluronic acid having improved intestinal absorption, for example, "chicken wool" which is a part of the body of a chicken or the like which is edible and relatively easily available. It has been found that the above-mentioned problems can be solved by selecting a compound of the formula (1), further applying a means for reducing the molecular weight of the hyaluronic acid contained therein, and using this as a food additive.
【００１２】すなわち、本発明は、重量平均分子量が1
0,000〜500,000 のヒアルロン酸を含有する、ヒアルロ
ン酸の腸管吸収性が向上した食品添加物（以下、本発明
食品添加物ともいう）、好ましくは高温・高圧処理を施
した鶏冠の蛋白分解酵素消化物からなる本発明食品添加
物、及びこの本発明食品添加物を含有する食品組成物
（以下、本発明食品組成物ともいう）を提供する発明で
ある。That is, according to the present invention, the weight average molecular weight is 1
A food additive containing hyaluronic acid of 0,000 to 500,000 and having improved intestinal absorption of hyaluronic acid (hereinafter also referred to as the food additive of the present invention), preferably a proteolytic enzyme digest of a cockscomb subjected to high temperature and high pressure treatment The present invention provides a food additive of the present invention, and a food composition containing the present food additive (hereinafter, also referred to as the present food composition).
【００１３】また、本発明食品添加物は、好ましくは、
少なくとも下記の工程を含む製造方法（以下、本発明製
造方法ともいう）により製造される食品添加物である。 工程１：鶏冠を蛋白分解酵素で消化し、この消化物を取
得する工程。 工程２：工程１で得られた鶏冠の蛋白分解酵素消化物
に、オートクレーブ処理等の高温・高圧処理を施し、こ
の処理物を取得して、上記食品添加物とする工程。The food additive of the present invention preferably comprises
It is a food additive produced by a production method including at least the following steps (hereinafter, also referred to as the production method of the present invention). Step 1: a step of digesting a cockscomb with a protease and obtaining the digest. Step 2: a step of subjecting the digested proteolytic enzyme of the cockscomb obtained in Step 1 to a high-temperature / high-pressure treatment such as an autoclave treatment to obtain the treated product and use it as the food additive.
【００１４】なお、本発明において、「鶏冠」とは、ニ
ワトリ等ある種のキジ目の鳥の頭上にある肉質の突起を
意味するものである。In the present invention, the term "cow blade" means a fleshy projection on the head of a certain pheasant bird such as a chicken.
【００１５】また、本発明において「食品」とは、食物
として摂取し得るものを意味し、固形物や液状物等、そ
の具体的な形態を問う概念ではない。In the present invention, the term "food" means something that can be ingested as food, and is not a concept as to the specific form of a solid or liquid.
【００１６】ここで、本発明の内容に最も近いと思われ
る先行技術について説明する。特開平５−１１１３６７
号公報には、蛋白質分解酵素で酵素分解してペプタイド
状にした蛋白質とヒアルロン酸を有する食品が記載され
ており、その製法として、鳥の鶏冠を蛋白質分解酵素で
分解する方法を含む工程が記載されている。しかしなが
ら、ヒアルロン酸の重量平均分子量を、10,000〜500,00
0 に調節して、ヒアルロン酸の腸管吸収性を高めた食品
添加物や、鶏冠の蛋白分解酵素消化物の高温・高圧処理
について、この公報中には、何らの記載も示唆もなされ
ていない。Here, the prior art which is considered to be closest to the contents of the present invention will be described. JP-A-5-111467
In the publication, a food having a protein and hyaluronic acid which is enzymatically decomposed with a protease and formed into a peptide is described, and a process including a method of decomposing a bird's cockscomb with a protease is described as a method for producing the same. Have been. However, the weight average molecular weight of hyaluronic acid is 10,000-500,00
This publication does not disclose or suggest any food additive in which the intestinal absorption of hyaluronic acid is adjusted to 0 to enhance the intestinal absorptivity, or the high-temperature and high-pressure treatment of a proteolytic enzyme digest of a cockscomb.
【００１７】また、特開平３−３５７７４号公報には、
動物性粘性物質を添加してなる飲食物が記載されてお
り、その起源として鶏冠が例示されている。また、この
粘性物質の調製法として、軟骨を用い、これをアルカリ
存在下で蒸煮して加水分解する方法が記載されている。
さらに、この調製法により、ヒアルロン酸を約１％含有
する粘性物質（おおよその分子量20,000〜100,000 ）製
造された旨が記載されている。しかしながら、ヒアルロ
ン酸の重量平均分子量を10,000〜500,000 に調節して、
ヒアルロン酸の腸管吸収性を高めた食品添加物や、鶏冠
の高温・高圧処理については、何らの記載も示唆もされ
ていない。Also, Japanese Patent Application Laid-Open No. 3-35774 discloses that
Foods and drinks to which animal viscous substances are added are described, and a cockscomb is exemplified as the source. Further, as a method for preparing this viscous substance, a method is described in which cartilage is used, and the cartilage is steamed and hydrolyzed in the presence of an alkali.
Furthermore, it is described that a viscous substance (approximate molecular weight of 20,000 to 100,000) containing about 1% of hyaluronic acid was produced by this preparation method. However, adjusting the weight average molecular weight of hyaluronic acid to 10,000-500,000,
There is no description or suggestion of a food additive that enhances the intestinal absorption of hyaluronic acid or high-temperature / high-pressure treatment of cockscomb.
【００１８】また、特開昭６１−４７４１８号公報に
は、コンドロイチン硫酸・ヒアルロン酸含有健康増進・
維持剤について記載されており、ヒアルロン酸、コンド
ロイチン硫酸、レシチン及び大豆等の蛋白質を含有する
組成物が記載されている。また、原料として鳥類の鶏冠
等が例示されており、酵素法、オートクレーブ法等の方
法による抽出についても例示されている。しかしなが
ら、ヒアルロン酸の重量平均分子量を10,000〜500,000
に調節して、ヒアルロン酸の腸管吸収性を向上させた食
品添加物や、鶏冠の蛋白分解酵素消化物を高温・高圧処
理することによって、この食品添加物を製造することに
ついては、何らの記載も示唆も認められない。Further, Japanese Patent Application Laid-Open No. 61-47418 discloses a health promotion method containing chondroitin sulfate and hyaluronic acid.
Maintenance agents are described, and compositions containing proteins such as hyaluronic acid, chondroitin sulfate, lecithin and soy are described. In addition, a bird's cockscomb is exemplified as a raw material, and extraction by a method such as an enzyme method or an autoclave method is also exemplified. However, the weight average molecular weight of hyaluronic acid is 10,000-500,000
No description is made about the production of this food additive by controlling the food additive with improved intestinal absorption of hyaluronic acid and the digestion of the proteolytic enzyme of the chicken crown by high temperature and high pressure. And no suggestion.
【００１９】[0019]
【発明の実施の形態】以下、本発明の実施の形態につい
て説明する。上述のように、本発明食品添加物は、重量
平均分子量が10,000〜500,000 、好ましくは同10,000〜
100,000 、より好ましくは同60,000〜80,000であるヒア
ルロン酸を含有する、ヒアルロン酸の腸管吸収性が向上
した食品添加物であり、好適には、鶏冠の蛋白分解酵素
消化物からなり、かつ、この消化物に含まれるヒアルロ
ン酸の重量平均分子量が、上記の重量平均分子量である
食品添加物である。Embodiments of the present invention will be described below. As described above, the food additive of the present invention has a weight average molecular weight of 10,000 to 500,000, preferably 10,000 to 500,000.
100,000, more preferably containing 60,000 to 80,000 hyaluronic acid, is a food additive with improved intestinal absorption of hyaluronic acid, preferably consisting of a digestive product of the proteolytic enzyme of cockscomb, and The weight average molecular weight of the hyaluronic acid contained in the food additive is a food additive having the above weight average molecular weight.
【００２０】本発明食品添加物として用いられる、腸管
吸収性が向上したヒアルロン酸を含む素材は、特に限定
されるものではないが、その安全性と経済性を鑑みる
と、ヒアルロン酸を非常に豊富に含有し、かつ、食用可
能な、鳥類の「鶏冠」を選択することが好ましい。この
「鶏冠」の供給源となる鳥類は、上述のようにある種の
キジ目に属する鳥類であり、ニワトリに限定されるもの
ではないが、その入手の圧倒的な容易性を鑑みると、ニ
ワトリを鶏冠の供給源とすることが好ましい。The material containing hyaluronic acid with improved intestinal absorption used as a food additive of the present invention is not particularly limited, but in view of its safety and economy, it is very rich in hyaluronic acid. It is preferable to select edible bird breeding cocks that are contained in edible corn. The birds that serve as the source of this “cock” are birds belonging to certain species of pheasants as described above, and are not limited to chickens, but in view of the overwhelming ease of obtaining them, chickens Is preferably the source of the cockscomb.
【００２１】この鶏冠には、ヒアルロン酸が非常に豊富
に含まれているが、その重量平均分子量は、１００万〜
２００万という高分子量であり、これをそのまま経口摂
取しても、腸管吸収は殆どなされない。The cockscomb contains a very abundant amount of hyaluronic acid, but its weight average molecular weight is 1,000,000 to
It has a high molecular weight of 2 million, and even if it is taken orally as it is, almost no intestinal absorption is achieved.
【００２２】しかしながら、本発明においては、鶏冠を
蛋白分解酵素で消化処理して、この消化物に、さらに高
温・高圧処理を施すことによって、上記の高分子量のヒ
アルロン酸を低分子化することができる。However, in the present invention, it is possible to reduce the molecular weight of the high-molecular-weight hyaluronic acid by digesting the cockscomb with a protease and subjecting the digest to a high-temperature and high-pressure treatment. it can.
【００２３】上記の蛋白分解酵素による消化処理を行う
前提として、鶏冠に対して公知の前処理を施すことが好
ましい。この前処理は、一般的な動物組織処理と同様
に、例えば、鶏冠を水に浸し、可能な限り血液を除去
し、一旦、熱水で煮沸する等により完了することができ
る。この前処理により、次の工程における蛋白分解酵素
による消化処理を容易に行うことができる。As a prerequisite for performing the digestion treatment with the above protease, it is preferable to perform a known pretreatment on the chicken crown. This pretreatment can be completed, for example, by immersing the cockscomb in water, removing blood as much as possible, and once boiling with hot water, as in general animal tissue treatment. By this pretreatment, the digestion treatment with the protease in the next step can be easily performed.
【００２４】このように、好ましくは上述のような前処
理を施した鶏冠に、蛋白分解酵素を作用させることによ
り、所望する鶏冠の蛋白分解酵素による消化物を得るこ
とができる。[0024] As described above, the digestion by the protease of the desired cockscomb can be obtained by allowing the protease to act on the cockscomb which is preferably subjected to the pretreatment as described above.
【００２５】この蛋白分解酵素処理工程において用いら
れ得る蛋白分解酵素は、通常公知の蛋白分解酵素を、単
独で又は組み合わせて用いることができる。かかる蛋白
分解酵素としては、例えば、プロナーゼ、アクチナー
ゼ、サモアーゼ、ペプシン、トリプシン等を例示するこ
とが可能であるが、基質特異性ができる限り広い蛋白分
解酵素を選択することが好ましい。この点において、上
記のプロナーゼ、アクチナーゼ、サモアーゼは、基質特
異性が広く、かつ、市販もされており、入手が比較的容
易であるという点において、本発明製造方法における蛋
白分解酵素処理工程において選択するのに好ましい蛋白
分解酵素である。As the protease which can be used in this protease treatment step, generally known proteases can be used alone or in combination. As such a protease, for example, pronase, actinase, samoase, pepsin, trypsin and the like can be exemplified, but it is preferable to select a protease having as wide a substrate specificity as possible. In this respect, the above-mentioned pronase, actinase, and samoase are selected in the proteolytic enzyme treatment step in the production method of the present invention in that they have wide substrate specificity, are commercially available, and are relatively easy to obtain. This is a preferred protease.
【００２６】また、上記の蛋白分解酵素処理工程におい
ては、選択した蛋白分解酵素の至適条件において、消化
処理を進行させることが好ましいことは勿論である。よ
り具体的には、選択した蛋白分解酵素の性質に応じた、
温度設定、ｐＨ設定等を行うことが好ましい。In the proteolytic enzyme treatment step, it is needless to say that the digestion treatment is preferably advanced under the optimal conditions for the selected proteolytic enzyme. More specifically, depending on the nature of the selected protease
It is preferable to perform temperature setting, pH setting, and the like.
【００２７】このようにして、蛋白分解処理工程を経た
鶏冠の消化物において、元来の蛋白質は、アミノ酸やペ
プチドに分解され、組織からヒアルロン酸をほぼ完全に
分離することが可能であり、後述する鶏冠中のヒアルロ
ン酸の低分子化処理の効率を向上させることができる。
かかる蛋白質処理工程は、効率的に鶏冠のヒアルロン酸
を抽出し、低分子化することを可能にするために必須の
工程である。[0027] In this manner, in the digest of chicken cop which has undergone the proteolytic treatment step, the original protein is decomposed into amino acids and peptides, and it is possible to almost completely separate hyaluronic acid from the tissue. The efficiency of the treatment for dehydrating hyaluronic acid in the cockscomb can be improved.
Such a protein treatment step is an essential step in order to efficiently extract the hyaluronic acid of the chicken wool and reduce the molecular weight.
【００２８】本発明製造方法においては、この蛋白分解
酵素処理工程に次いで、上述のように、ヒアルロン酸の
低分子化工程を行うことが必要である。上記の蛋白分解
酵素処理工程が終了した時点においても、鶏冠の消化処
理物におけるヒアルロン酸の重量平均分子量は、１００
万〜２００万であり、既報（特公昭60-9042 号公報、特
公昭61-21241号公報）通り、この時点においては、ヒア
ルロン酸の低分子化は起こっていない。In the production method of the present invention, it is necessary to carry out the step of reducing the molecular weight of hyaluronic acid, as described above, following the step of treating the protease. Even when the above protease treatment step is completed, the weight-average molecular weight of hyaluronic acid in the digested product of the cockscomb is 100%.
As described previously (JP-B-60-9042 and JP-B-61-21241), at this point, the molecular weight of hyaluronic acid has not been reduced.
【００２９】ヒアルロン酸の低分子化手段としては、酸
処理、アルカリ処理又はヒアルロニダーゼ等の特定の酵
素処理等が既に知られている。しかしながら、これらの
低分子化処理工程においては、本品に多くの塩が混入し
たり、着色したり、さらにはヒアルロン酸の分子量を一
定の分子量にコントロールしにくい等の問題が認められ
る。As means for reducing the molecular weight of hyaluronic acid, acid treatment, alkali treatment, treatment with a specific enzyme such as hyaluronidase and the like have already been known. However, in these low-molecular-weight treatment steps, there are problems such as mixing of a large amount of salt into the product, coloring, and difficulty in controlling the molecular weight of hyaluronic acid to a constant molecular weight.
【００３０】本発明製造方法においては、このヒアルロ
ン酸の低分子化手段として、高温・高圧処理を選択する
ことにより、ヒアルロン酸の分子量を調節することが容
易である等、上記の他の手段によるヒアルロン酸の低分
子化工程における不都合を回避することが可能になる。
また、高温・高圧処理を施すことにより、滅菌処理を同
時に行うことができる（上記の高温・高圧処理以外の方
法を選択する場合には、別個に滅菌処理を行う必要があ
る）。In the production method of the present invention, a high-temperature / high-pressure treatment is selected as a means for lowering the molecular weight of hyaluronic acid, so that the molecular weight of hyaluronic acid can be easily adjusted. It is possible to avoid inconvenience in the step of dehydrating hyaluronic acid.
In addition, sterilization can be performed simultaneously by performing high-temperature and high-pressure treatment (if a method other than the above-described high-temperature and high-pressure treatment is selected, sterilization must be performed separately).
【００３１】かかる高温・高圧処理の条件は、処理する
鶏冠の消化物の量や濃度等に応じて適宜選択するべきも
のであり、特に限定されるものではないが、概ね、処理
温度が１１０〜１３０℃、処理圧力が１〜２kg/cm2、処
理時間が１０〜６００分程度が好ましい。これらの条件
よりも処理条件が過酷であると、ヒアルロン酸が過度に
分解して、目的とする重量平均分子量よりも低分子化す
る傾向や着色する傾向が強くなり好ましくなくなる場合
が多く、逆にこれらの条件よりも緩和した条件である
と、ヒアルロン酸の低分子化が十分に進行しない傾向が
強くなり好ましくなくなる場合が多い。The conditions for the high-temperature and high-pressure treatment are to be appropriately selected according to the amount and concentration of digests of the chicken crown to be treated, and are not particularly limited. It is preferable that the temperature is 130 ° C., the processing pressure is 1-2 kg / cm 2 , and the processing time is about 10-600 minutes. When the treatment conditions are more severe than these conditions, hyaluronic acid is excessively decomposed, and the tendency to lower the molecular weight and the tendency to color more than the intended weight average molecular weight often becomes unfavorable, and conversely If the conditions are less relaxed than these conditions, the tendency of hyaluronic acid to become lower in molecular weight tends to be insufficient, and is often unfavorable.
【００３２】また、この高温・高圧処理を、鶏冠の消化
物に施す具体的な手段は、所望する高温・高圧処理条件
を与え得る手段であれば特に限定されず、例えば、オー
トクレーブ処理、圧力窯処理等を選択することができる
が、条件設定が容易である点や装置の取扱いが比較的簡
単である点、大量処理が容易である点等を鑑みるとオー
トクレーブ処理を選択することが好ましい。このように
して、上記の蛋白分解酵素処理によって得られた、鶏冠
の消化物におけるヒアルロン酸の低分子化処理工程を完
了することができる。The specific means for applying the high-temperature and high-pressure treatment to the digest of the chicken crown is not particularly limited as long as it can provide desired high-temperature and high-pressure processing conditions. For example, autoclave treatment, pressure kiln Although the processing and the like can be selected, it is preferable to select the autoclave processing in view of the fact that the condition setting is easy, the handling of the apparatus is relatively simple, and the mass processing is easy. In this way, the process of degrading the molecular weight of hyaluronic acid in the digested chicken crown obtained by the proteolytic enzyme treatment can be completed.
【００３３】好ましくは、これらの２工程（蛋白分解酵
素工程及びヒアルロン酸低分子化工程）を少なくとも含
む工程によって鶏冠を処理することにより得られる、含
有されるヒアルロン酸の重量平均分子量が、10,000〜50
0,000 、好適には同10,000〜100,000 、より好適には同
60,000〜80,000である生成物が、本発明食品添加物であ
る。そして、かかる本発明食品添加物を製造する、この
２工程を少なくとも含む製造方法が、本発明製造方法で
ある。なお、生成物中のヒアルロン酸の重量平均分子量
は、通常公知の方法、例えば、ゲルクロマトグラフィ
ー、粘度の測定等により容易に行うことができる。Preferably, the weight average molecular weight of the contained hyaluronic acid obtained by treating the cockscomb by a step including at least these two steps (protease step and hyaluronic acid depolymerizing step) is 10,000 to 50
0,000, preferably 10,000 to 100,000, more preferably
A product that is between 60,000 and 80,000 is a food additive of the present invention. And the manufacturing method including at least these two steps for manufacturing the food additive of the present invention is the manufacturing method of the present invention. The weight average molecular weight of hyaluronic acid in the product can be easily determined by a generally known method, for example, gel chromatography, measurement of viscosity, and the like.
【００３４】上述のように、好適には、本発明製造方法
によって得られる本発明食品添加物は、これをヒトが経
口摂取した場合に、この添加物中に含まれるヒアルロン
酸が容易に腸管吸収され、このヒアルロン酸の作用によ
る、便秘解消等という形で現される体調調整機能や、美
肌作用等が容易に発揮され得る。As described above, preferably, the food additive of the present invention obtained by the production method of the present invention easily absorbs hyaluronic acid contained in the additive when ingested by humans. In addition, the action of hyaluronic acid can easily exert a physical condition adjusting function expressed in the form of, for example, constipation resolution, a beautiful skin effect, and the like.
【００３５】本発明食品添加物は、後述するように、文
字通り、食品組成物中に含有される「食品添加物」とし
て用いることの他に、本発明食品添加物を、そのまま食
品として用いることも可能である。As will be described later, the food additive of the present invention can be used literally as a “food additive” contained in a food composition, or the food additive of the present invention can be used as a food as it is. It is possible.
【００３６】本発明食品添加物は、具体的に用いられる
態様に応じて、通常食品において施される公知の処理、
例えば、凍結乾燥処理、エタノール添加による沈澱法や
噴霧乾燥等による粉末化処理、液状化処理等を施すこと
ができる。また、本発明食品組成物の１日当り摂取量
は、本発明食品組成物の安全性が非常に高度であるため
に、特に限定されるものではなく、任意に選択され得
る。[0036] The food additive of the present invention may be prepared by a known process usually applied to foods, depending on the mode of use.
For example, a freeze-drying process, a powdering process by a precipitation method by adding ethanol, a spray drying process, or the like, a liquefaction process, and the like can be performed. The daily intake of the food composition of the present invention is not particularly limited and can be arbitrarily selected, since the safety of the food composition of the present invention is very high.
【００３７】本発明は、さらに上記の本発明食品添加物
を含有する本発明食品組成物を提供する。本発明食品組
成物における、本発明食品添加物の添加量は、本発明食
品組成物の具体的な形態や目的等に応じて適宜選択され
るべきものであり、特に限定されないが、ヒアルロン酸
換算で、概ね組成物全体に対して０．５〜３０．０重量
％が好ましく、特に好ましくは、同１．０〜５．０重量
％である。The present invention further provides a food composition of the present invention containing the above-mentioned food additive of the present invention. In the food composition of the present invention, the amount of the food additive of the present invention should be appropriately selected according to the specific form and purpose of the food composition of the present invention, and is not particularly limited. In general, the content is preferably 0.5 to 30.0% by weight, particularly preferably 1.0 to 5.0% by weight, based on the whole composition.
【００３８】この配合量が、ヒアルロン酸換算で組成物
全体の０．５重量％未満であると、通常人が摂取する食
品の量を勘案すると、期待される美肌効果、体調調整機
能等を実質的に発揮させることが困難になり、好ましく
なく、逆に、同３０．０重量％を超えて配合すると、ヒ
アルロン酸の吸湿性が顕在化し、種々の加工がしづらく
なる傾向が強くなる。しかしながら、ヒアルロン酸換算
で組成物全体の３０．０重量％を超えてヒアルロン酸を
配合することは、本発明食品組成物の体調調整機能や美
肌作用に何らの悪影響を与えるものではなく、かえっ
て、これらの機能や作用を増強する場合もあることか
ら、本発明は、同３０．０重量％を超えるヒアルロン酸
の配合を、何ら排除するものではない。When the amount is less than 0.5% by weight of the whole composition in terms of hyaluronic acid, the expected skin effect, physical condition adjusting function, etc. can be substantially achieved in consideration of the amount of food normally consumed by humans. In contrast, if the amount is more than 30.0% by weight, hyaluronic acid becomes more hygroscopic and the processing tends to be difficult. However, the addition of hyaluronic acid in an amount exceeding 30.0% by weight of the total composition in terms of hyaluronic acid does not have any adverse effect on the physical condition adjusting function and the beautiful skin effect of the food composition of the present invention. Since these functions and actions may be enhanced in some cases, the present invention does not exclude the inclusion of hyaluronic acid exceeding 30.0% by weight.
【００３９】このようにして、経口摂取することで、本
発明食品添加物に配合されているヒアルロン酸の作用に
より、便秘解消等という形で現される体調調整機能や、
美肌作用等を容易に発揮し得る、本発明食品添加物を含
有する本発明食品組成物が提供される。In this way, when taken orally, the effect of hyaluronic acid contained in the food additive of the present invention allows the body to adjust its physical condition, such as relieving constipation.
The present invention provides a food composition containing the food additive of the present invention, which is capable of easily exhibiting a beautiful skin effect.
【００４０】本発明食品組成物においては、その所期の
効果を妨げない限り、通常、食品において添加される、
一般的成分、例えば、充填剤、増量剤、結合剤、崩壊
剤、滑沢剤、調味剤、香料、甘味料、酸味料、食塩、各
種栄養成分、防腐剤、着色剤、保存剤等を配合すること
が可能である。また、本発明食品組成物の採り得る形態
は、特に限定されるものではなく、米飯、パン、麺、菓
子、飲料、スープ、錠剤状食品、顆粒状食品、液状食
品、カプセル状食品や、その他の形態を採ることが可能
である。In the food composition of the present invention, the food composition is usually added to a food unless the intended effect is hindered.
Contains general ingredients such as fillers, extenders, binders, disintegrants, lubricants, seasonings, flavors, sweeteners, acidulants, salt, various nutrients, preservatives, coloring agents, preservatives, etc. It is possible to The form that the food composition of the present invention can take is not particularly limited, and rice, bread, noodles, confectionery, beverages, soups, tablet foods, granular foods, liquid foods, capsule foods, and others It is possible to take the form of
【００４１】なお、本発明食品組成物において、所望さ
れる体調調整機能や美肌効果等の一層の向上を図る等の
理由から、現在、医薬品や化粧品において用いられてい
るヒアルロン酸の精製品を添加することも可能である。In the food composition of the present invention, pure hyaluronic acid, which is currently used in pharmaceuticals and cosmetics, is added for the purpose of further improving the desired physical condition adjusting function and skin effect. It is also possible.
【００４２】[0042]
【実施例】以下、本発明を実施例により、さらに具体的
に説明するが、かかる実施例により、本発明の技術的範
囲が限定されるものではない。また、本実施例において
配合量は、特に断わらない限り、配合される成分の配合
される対象に対する重量％で表示される。EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples, but the technical scope of the present invention is not limited by these examples. In addition, in this example, unless otherwise specified, the compounding amount is expressed in terms of% by weight based on the compounding target of the compounded compound.
【００４３】〔試験例〕分子量が異なるヒアルロン酸に
おける腸管吸収試験14 Ｃで標識したヒアルロン酸（重量平均分子量100 万）
（14Ｃ-HA100と称する）と、14Ｃで標識したヒアルロン
酸（重量平均分子量10万）（14Ｃ-HA10 と称する）を調
製し、2mg/kgの割合でゾンデを使用して、体重2.6 〜2.
8Kg の日本白色在来種ウサギ（雌）３匹ずつに強制的に
経口投与した。投与後６時間に、頸動脈からできるだけ
採血し、この血液から血清を分離した。この血清は、分
析するまで-80 ℃で凍結保存した。[Test Example] Intestinal absorption test with hyaluronic acid having different molecular weights Hyaluronic acid labeled with 14 C (weight average molecular weight 1,000,000)
(Referred to as 14 C-HA100) and 14 C-labeled hyaluronic acid (weight-average molecular weight 100,000) (referred to as 14 C-HA10) were prepared at a rate of 2 mg / kg using a sonde to give a body weight of 2.6. ~ 2.
Three 8 kg Japanese white native rabbits (females) were forcibly administered orally. Six hours after the administration, blood was collected from the carotid artery as much as possible, and serum was separated from the blood. This serum was stored frozen at -80 ° C until analysis.
【００４４】14Ｃ-HA100及び14Ｃ-HA10 はそれぞれ21.3
kBq/mg dry wt.(576nCi/mg dry wt.) であった。なお、
ウサギの回腸及び盲腸内容物からヒアルロン酸分解菌を
３株分離し、Bacterroides属に属する菌株として同定し
た。これらの３株は、いずれも24時間で培地中のヒアル
ロン酸を完全に分解すること、６時間で低分子化は起こ
すがヒアルロン酸としてまだ確認されることが判明した
ので、上記の採血は、標識したヒアルロン酸の投与６時
間後に行った。血清中の放射能の測定は、0.5mL の血清
を20mLのハイオニックフロー（パッカードジャパン株式
会社、東京）と混合し、37℃で24時間インキュベーショ
ンすることにより、化学発光を減衰させることにより行
った。次に、放射能を液体シンチレーション計測器（ト
リカーブ4640、パッカードジャパン株式会社）で測定し
た。その結果を第１表に示す。 14 C-HA100 and 14 C-HA10 each had 21.3
kBq / mg dry wt. (576 nCi / mg dry wt.). In addition,
Three strains of hyaluronic acid-degrading bacteria were isolated from the contents of rabbit ileum and cecum and identified as strains belonging to the genus Bacterroides. All three strains were found to completely degrade hyaluronic acid in the culture medium in 24 hours and to be degraded in 6 hours, but still confirmed as hyaluronic acid. This was performed 6 hours after administration of the labeled hyaluronic acid. The measurement of radioactivity in serum was performed by attenuating chemiluminescence by mixing 0.5 mL of serum with 20 mL of Hionic Flow (Packard Japan, Tokyo) and incubating at 37 ° C for 24 hours. . Next, radioactivity was measured with a liquid scintillation counter (Tricarb 4640, Packard Japan KK). Table 1 shows the results.
【００４５】 第 １ 表 ─────────────────────────────────── ヒアルロン酸 血清中放射能濃度（μg Eq.of 14Ｃ-HA/mL） ─────────────────────────────────── 14Ｃ-HA100 0.334 ± 0.028 14Ｃ-HA10 1.553 ± 0.123 ───────────────────────────────────Table 1 ─────────────────────────────────── Hyaluronic acid Serum radioactivity concentration (μg Eq.of 14 C-HA / mL) ─────────────────────────────────── 14 C-HA100 0.334 ± 0.028 14 C-HA10 1.553 ± 0.123 ───────────────────────────────────
【００４６】この結果により、重量平均分子量が１００
万の高分子のヒアルロン酸は、容易に腸管吸収されない
が、同分子量が１０万の低分子のヒアルロン酸は、容易
に腸管吸収されることが明らかになった。According to the result, the weight average molecular weight was 100
It was revealed that 10,000 high molecular weight hyaluronic acids are not easily absorbed into the intestinal tract, whereas low molecular weight hyaluronic acid having the same molecular weight of 100,000 is easily absorbed into the intestinal tract.
【００４７】〔実施例１〕本発明食品添加物の製造
（１） ニワトリの鶏冠15kgに、水30L を加え、30分煮沸した。
その後、ミンチにかけてサモアーゼ（大和化成製）を30
g 加えて、65℃で１晩、上記鶏冠に蛋白分解酵素である
サモアーゼによる消化処理を施した。得られた消化液の
一部を採取し、濾過して、ゲルクロマトグラフィ〔カラ
ム：TSK gel G6000PWXL（東ソー製）〕で、かかる消化
液に含まれているヒアルロン酸の分子量を測定すると、
重量平均分子量で、１１０万であった。Example 1 Production of the food additive of the present invention (1) To 15 kg of chicken cockscomb, 30 L of water was added, and the mixture was boiled for 30 minutes.
After that, sprinkle with minced samoase (manufactured by Daiwa Kasei) for 30 minutes.
g In addition, the above-mentioned cockscomb was digested with a protease, samoase, at 65 ° C overnight. A part of the obtained digestive juice was collected, filtered, and subjected to gel chromatography [column: TSK gel G6000PW XL (manufactured by Tosoh)] to measure the molecular weight of hyaluronic acid contained in the digestive juice.
The weight average molecular weight was 1.1 million.
【００４８】次いで、上記の消化液に対して、オートク
レーブを用いて、121 ℃で１時間（１Kg/cm2）の高温・
高圧処理を行った。かかる高温・高圧処理後、消化液中
の不溶物を濾過して、得られた濾液をスプレードライで
乾燥した。Next, the digested solution was heated at 121 ° C. for 1 hour (1 kg / cm 2 ) using an autoclave.
High pressure treatment was performed. After the high-temperature and high-pressure treatment, insolubles in the digested solution were filtered, and the obtained filtrate was dried by spray drying.
【００４９】このようにして得られた本発明食品添加物
の内容を解析したところ、第２表に示す結果が得られ
た。なお、ヒアルロン酸含量の解析は、Bitter-Muir に
よるカルバゾール硫酸法で行った（Analytical Biochem
istry 4, 330-334,1962）。When the contents of the food additive of the present invention thus obtained were analyzed, the results shown in Table 2 were obtained. The analysis of the hyaluronic acid content was performed by the carbazole sulfate method using Bitter-Muir (Analytical Biochem.
istry 4, 330-334,1962).
【００５０】 第 ２ 表 ───────────────────────── 項目 結果 ───────────────────────── 収量 1800g pH 6.0 重金属 20ppm以下 砒素 2ppm以下 ヒアルロン酸含量 6.5% ヒアルロン酸重量平均分子量 60,000 ─────────────────────────Table 2 ───────────────────────── Item Result ─────────────────収 量 Yield 1800g pH 6.0 Heavy metal 20ppm or less Arsenic 2ppm or less Hyaluronic acid content 6.5% Hyaluronic acid weight average molecular weight 60,000 ───────────────────── ────
【００５１】〔実施例２〕本発明食品添加物の製造
（２） ニワトリの鶏冠15kgに、水30L を加え、30分煮沸した。
その後、ミンチにかけてサモアーゼ（大和化成製）を30
g 加えて、65℃で１晩消化した。次いで、この消化液を
オートクレーブで、121 ℃で１時間の高温・高圧処理を
行った。この高温・高圧処理後、消化液中の不溶物を濾
過して、得られた濾液30L に、食塩３％を含むエタノー
ル60L を添加して、沈澱を得た。この沈澱を遠心して、
取り出し水５L に溶解後、エタノール22.5L を添加して
白色沈澱を得た。この沈澱物を濾過して、減圧下で乾燥
した。このようにして得られた本発明食品添加物の内容
を解析したところ、第３表に示す結果が得られた。Example 2 Production of the food additive of the present invention (2) To 15 kg of chicken cockscomb, 30 L of water was added, and the mixture was boiled for 30 minutes.
After that, sprinkle with minced samoase (manufactured by Daiwa Kasei) for 30 minutes.
g In addition, digestion was performed at 65 ° C. overnight. Then, the digested solution was subjected to a high-temperature and high-pressure treatment at 121 ° C. for 1 hour in an autoclave. After the high-temperature and high-pressure treatment, insolubles in the digested solution were filtered, and to 30 L of the obtained filtrate, 60 L of ethanol containing 3% of sodium chloride was added to obtain a precipitate. Centrifuge this precipitate,
After taking out and dissolving in 5 L of water, 22.5 L of ethanol was added to obtain a white precipitate. The precipitate was filtered and dried under reduced pressure. When the content of the food additive of the present invention thus obtained was analyzed, the results shown in Table 3 were obtained.
【００５２】 第 ３ 表 ───────────────────────── 項目 結果 ───────────────────────── 収量 150g pH 5.9 重金属 20ppm以下 砒素 2ppm以下 ヒアルロン酸含量 76.9% ヒアルロン酸重量平均分子量 80,000 ─────────────────────────Table 3 ───────────────────────── Item Result ─────────────────収 量 Yield 150g pH 5.9 Heavy metal 20ppm or less Arsenic 2ppm or less Hyaluronic acid content 76.9% Hyaluronic acid weight average molecular weight 80,000 ───────────────────── ────
【００５３】これらの実施例の結果により、本発明食品
添加物におけるヒアルロン酸の重量平均分子量は、10,0
00〜500,000 であり、上述の試験例の結果を鑑みると、
本発明食品添加物を摂取した場合の、ヒアルロン酸の腸
管吸収が良好であることは明らかである。From the results of these examples, it was found that the weight average molecular weight of hyaluronic acid in the food additive of the present invention was 10,0
00-500,000, considering the results of the above test examples,
It is clear that hyaluronic acid has good intestinal absorption when the food additive of the present invention is ingested.
【００５４】〔実施例３〕本発明食品組成物の製造例及
びこれを用いたパネルテスト （１）本発明食品組成物の調製 以下の内容の本発明食品組成物〔検体Ａ，検体Ｂ及び検
体Ｃ（対照）〕を調製した（各配合成分をそれぞれ混和
後、これを常法により顆粒化し、これをカプセルに充填
した）。Example 3 Production Example of the Food Composition of the Present Invention and Panel Test Using the Same (1) Preparation of the Food Composition of the Present Invention The food composition of the present invention [Sample A, Sample B and Sample C (control)] (each blended component was mixed, granulated by a conventional method, and filled into capsules).
【００５５】 検体Ａ 配合成分 配合量（mg） 実施例１で製造した本発明食品添加物 ５００ （ヒアルロン酸として３０mg） レシチン ２００ 大豆蛋白 ８００Sample A Compounding Components (mg) Food additive of the present invention produced in Example 1 (30 mg as hyaluronic acid) Lecithin 200 Soy protein 800
【００５６】 検体Ｂ 配合成分 配合量（mg） 実施例２で製造した本発明食品添加物 ３９ （ヒアルロン酸として３０mg） レシチン ２９２ 大豆蛋白 １１６９Sample B Blending Amount (mg) Food additive of the present invention 39 (30 mg as hyaluronic acid) produced in Example 2 Lecithin 292 Soy protein 1169
【００５７】 検体Ｃ 配合成分 配合量（mg） レシチン ３００ 大豆蛋白 １２００Sample C Compounding Components (mg) Lecithin 300 Soy Protein 1200
【００５８】（２）パネルテスト 本発明食品組成物が経口摂取された後、いかなる経過を
経て美肌効果を示すことになるのかについての詳細は知
られていない。しかしながら、この美肌効果には、少な
くとも、第一に、体調調整機能（ヒアルロン酸の組織修
復作用、鎮痛作用、保湿・保護作用等による便秘解消機
能）が向上すること、第二に、ヒアルロン酸が体内で吸
収され、血中に入ることによる細胞及び組織機能の活性
化が大きく関与していることは確実視されている。そこ
で、これら２種類の作用について、上記の検体におい
て、パネルテストを行った。(2) Panel test It is not known in detail how the food composition of the present invention will show a beautiful skin effect after being orally ingested. However, this beautiful skin effect has at least, firstly, an improvement in the physical condition adjustment function (function of relieving constipation by a tissue repairing action, an analgesic action, a moisturizing / protecting action of hyaluronic acid), and secondly, hyaluronic acid has It is certainly believed that the activation of cell and tissue functions by being absorbed in the body and entering the blood is greatly involved. Therefore, a panel test was performed on these two types of effects on the above-mentioned samples.
【００５９】便秘に関するパネルテスト 便秘で苦しんでいる年齢１８〜６０歳の女性で、便通が
週１回の人１５人及び同週２回の人１５人に、それぞれ
１回当り１５００mgの上記の検体を、１日に３回、連続
７日間服用させた。その結果を、第４表に示す。Panel test on constipation A female aged 18 to 60 years old who suffers from constipation and who has a bowel movement of 15 times twice a week and 15 times twice a week was given 1500 mg of the above sample each time. Was taken three times a day for seven consecutive days. Table 4 shows the results.
【００６０】 第 ４ 表 ──────────────────────────────────── 週１回の人 週２回の人 ────────────────────────────── 検体Ａ 検体Ｂ 検体Ｃ 検体Ａ 検体Ｂ 検体Ｃ ──────────────────────────────────── ５人 ５人 ５人 ５人 ５人 ５人 ──────────────────────────────────── 改善＊１ ４ ２ ０ ３ １ ０ ──────────────────────────────────── 改良＊２ １ ２ １ １ ２ ０ ──────────────────────────────────── 変化なし ０ １ ４ １ ２ ５ ──────────────────────────────────── 注） ＊１：服用開始２〜３日目から毎日通じがある人 ＊２：服用開始３日目から隔日又は毎日通じのある人Table 4 人 Once a week twice a week ────────────────────────────── Sample A Sample B Sample C Sample A Sample B Sample C ────── ────────────────────────────── 5 people 5 people 5 people 5 people 5 people 5 people ────────改善 Improvement * 1 4 2 3 0改良 Improvement * 2 1 2 1 1 2 0 ──────────────────な し No change 0 1 4 1 2 5 ──────────────────────── ───── ────── Note) * 1: there is a through every day from taking the start of two to three days people * 2: a person from taking the start of the third day of the through every other day or every day
【００６１】美肌効果に関するパネルテスト 上記の便秘に関するパネルテストを行った後、同じパネ
ルに、同じ検体を、同じ服用条件で、引き続いて８週間
摂食させた。この試験期間終了後、パネル全員に対して
アンケート調査を試みた。その結果を、第５表に示す。Panel test on skin effect After the above-mentioned panel test on constipation, the same panel was fed with the same sample under the same dosage condition for 8 weeks. After this test period, a questionnaire survey was conducted for all the panels. Table 5 shows the results.
【００６２】 第 ５ 表 ──────────────────────────────────── 肌の状態が良い ８人 肌の状態がやや良い ２８人 変化なし ５人 わからない ４人 ──────────────────────────────────── 肌の状態が良いと回答したパネルと、肌の状態がやや良
いと回答したパネルを合わせて、パネル全体の８０％で
あった。Table 5 ８ Good skin condition 8 people Skin Somewhat good 28 people No change 5 people I don't know 4 people A total of 80% of the total panel includes the panel which answered that the skin condition was good and the panel which answered that the skin condition was slightly good.
【００６３】この実施例３の結果により、本発明食品組
成物を摂取することにより、体内にヒアルロン酸が効率
良く吸収され、かかるヒアルロン酸の作用により、便秘
解消機能等の体調調整機能と、美肌機能が発揮されるこ
とが明らかになった。 また、この結果により、本発明
食品添加物自体にも、同様の体調調整機能と、美肌機能
が発揮されることは明らかである。According to the results of Example 3, when the food composition of the present invention is ingested, hyaluronic acid is efficiently absorbed into the body. By the action of hyaluronic acid, a physical condition adjusting function such as a constipation relieving function and a beautiful skin are obtained. It became clear that the function was exhibited. Further, it is apparent from the results that the food additive of the present invention itself also exerts a similar physical condition adjusting function and a beautiful skin function.
【００６４】[0064]
【発明の効果】本発明により、ヒアルロン酸の腸管吸収
が良好な、その摂取により優れた便秘解消等の体調調整
機能や美肌効果等を発揮し得る食品添加物、及びこの食
品添加物の製造方法、並びにこの食品添加物を含有す
る、美肌効果等を発揮し得る食品組成物が提供される。INDUSTRIAL APPLICABILITY According to the present invention, a food additive which has good intestinal absorption of hyaluronic acid and which can exert a physical condition adjusting function such as constipation relieving and a beautiful skin effect by ingestion thereof, and a method for producing this food additive And a food composition containing the food additive and capable of exhibiting a beautiful skin effect and the like.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.7 識別記号 ＦＩ テーマコート゛(参考） Ａ６１Ｐ 1/10 Ａ６１Ｋ 31/00 ６０１Ｆ Ａ６１Ｋ 31/728 31/715 ６１１ Ｆターム(参考） 4B018 LE03 MD10 MD27 MD69 MF01 MF04 MF12 4B042 AC04 AD39 AE01 AG07 AH02 AP02 AP06 AP27 4C083 AA071 AA072 AD331 AD332 AD412 AD471 AD472 AD572 CC50 EE12 4C086 AA01 AA02 AA04 EA25 MA41 MA52 NA11 ZB21 ──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. 7 Identification symbol FI Theme coat ゛ (Reference) A61P 1/10 A61K 31/00 601F A61K 31/728 31/715 611 F Term (Reference) 4B018 LE03 MD10 MD27 MD69 MF01 MF04 MF12 4B042 AC04 AD39 AE01 AG07 AH02 AP02 AP06 AP27 4C083 AA071 AA072 AD331 AD332 AD412 AD471 AD472 AD572 CC50 EE12 4C086 AA01 AA02 AA04 EA25 MA41 MA52 NA11 ZB21
Claims (6)
ルロン酸を含有する、ヒアルロン酸の腸管吸収性が向上
した食品添加物。1. A food additive containing hyaluronic acid having a weight average molecular weight of 10,000 to 500,000 and having improved intestinal absorption of hyaluronic acid.
項１記載の食品添加物。2. The food additive according to claim 1, comprising a proteolytic enzyme digest of a cockscomb.
り製造される、請求項２記載の食品添加物： 工程１：鶏冠を蛋白分解酵素で消化し、この消化物を取
得する工程； 工程２：工程１で得られた鶏冠の蛋白分解酵素消化物を
高温・高圧処理し、この処理物を取得して、上記食品添
加物とする工程。3. The food additive according to claim 2, which is produced by a production method comprising at least the following steps: Step 1: digesting a cockscomb with a protease and obtaining the digest; Step 2: A step of subjecting the digested proteolytic enzyme of the cockscomb obtained in step 1 to high-temperature and high-pressure treatment, obtaining the treated product, and using it as the food additive.
る、請求項３記載の食品添加物。4. The food additive according to claim 3, wherein the high temperature / high pressure treatment is an autoclave treatment.
食品添加物を含有する食品組成物。5. A food composition containing the food additive according to any one of claims 1 to 4.
の食品添加物の製造方法： 工程１：鶏冠を蛋白分解酵素で消化し、この消化物を取
得する工程； 工程２：工程１で得られた鶏冠の蛋白分解酵素消化物を
高温・高圧処理し、この処理物を取得して、上記食品添
加物とする工程。6. The method for producing a food additive according to claim 2, comprising at least the following steps: Step 1: digesting a cockscomb with a protease and obtaining the digested product; Step 2: obtaining in Step 1 A process of subjecting the digested proteolytic enzyme digest of the cockscomb to high temperature and high pressure, obtaining the treated product, and using the processed product as the food additive.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29303898A JP4097335B2 (en) | 1998-09-30 | 1998-09-30 | Food additive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29303898A JP4097335B2 (en) | 1998-09-30 | 1998-09-30 | Food additive |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007267988A Division JP4227657B2 (en) | 2007-10-15 | 2007-10-15 | Food additive for skin care |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2000102362A true JP2000102362A (en) | 2000-04-11 |
| JP2000102362A5 JP2000102362A5 (en) | 2005-11-10 |
| JP4097335B2 JP4097335B2 (en) | 2008-06-11 |
Family
ID=17789695
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP29303898A Expired - Fee Related JP4097335B2 (en) | 1998-09-30 | 1998-09-30 | Food additive |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4097335B2 (en) |
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| JP2002356432A (en) * | 2001-03-27 | 2002-12-13 | Q P Corp | Oral skin-improving agent, method for improving skin and food composition for improving skin |
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| WO2006101030A1 (en) * | 2005-03-22 | 2006-09-28 | Q.P. Corporation | Low molecular weight hyaluronic acid and/or salt thereof, method for producing same, and cosmetic preparation and food composition containing same |
| EP1707578A3 (en) * | 2005-02-15 | 2006-10-18 | Collagen Nutraceuticals, Inc. | Preparation of low molecular weight hyaluronic acid as a food supplement |
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002145800A (en) * | 2000-11-13 | 2002-05-22 | Raimu:Kk | Wound medicine |
| JP2002356432A (en) * | 2001-03-27 | 2002-12-13 | Q P Corp | Oral skin-improving agent, method for improving skin and food composition for improving skin |
| US6566346B2 (en) | 2001-03-27 | 2003-05-20 | Q.P. Corporation | Oral skin improving agent, skin improving method, and food composition for improving skin |
| US7524815B2 (en) * | 2001-04-03 | 2009-04-28 | Nestec S.A. | Osteoprotegerin in milk |
| JP2006036666A (en) * | 2004-07-26 | 2006-02-09 | Japan Science & Technology Agency | Expression inducer for chemokine receptor ccr10 |
| JP4528898B2 (en) * | 2004-07-26 | 2010-08-25 | 独立行政法人科学技術振興機構 | Chemokine receptor CCR10 expression inducer |
| EP1707578A3 (en) * | 2005-02-15 | 2006-10-18 | Collagen Nutraceuticals, Inc. | Preparation of low molecular weight hyaluronic acid as a food supplement |
| WO2006101030A1 (en) * | 2005-03-22 | 2006-09-28 | Q.P. Corporation | Low molecular weight hyaluronic acid and/or salt thereof, method for producing same, and cosmetic preparation and food composition containing same |
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| JP4712746B2 (en) * | 2006-09-15 | 2011-06-29 | キユーピー株式会社 | Acidic beverage |
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| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
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| LAPS | Cancellation because of no payment of annual fees |