JP1668100S - Sample separation device - Google Patents

Sample separation device

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Publication number
JP1668100S
JP1668100S JP2019025790F JP2019025790F JP1668100S JP 1668100 S JP1668100 S JP 1668100S JP 2019025790 F JP2019025790 F JP 2019025790F JP 2019025790 F JP2019025790 F JP 2019025790F JP 1668100 S JP1668100 S JP 1668100S
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liquid
sample
microparticles
storage section
collected
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JP2019025790F
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Japanese (ja)
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Abstract

本物品は、細胞、マイクロビーズ等の微小粒子の分取を行うフローサイトメトリーに用いる、使い捨て可能なデバイスである。当該デバイスは、閉鎖型セルソータの装置上にセットして用いられるものであり、各部位が密閉連結され、これらが一体不可分で用いられることにより、外気に接することなく目的とする回収されるべき微小粒子の分取を行うことができる。当該デバイスは、各部位を示す参考正面図に示すように、マイクロ流路チップ100の他に、サンプル収容部201、プレサンプル収容部2011、フィルタ部202、目標サンプル貯留部203、廃棄部204、シース液収容部205、バッファ液収容部206、ダンパー207、及び圧力計センサ208を備えており、これら各部位はチューブによって連結されている。当該デバイスにおいて、プレサンプル収容部2011に導入された微小粒子を含む液体(サンプル液)はフィルタ部202を介してサンプル収容部201に移動し、マイクロ流路チップ100内に導入され、回収されるべき微小粒子とそうでない微小粒子とに分取される。当該マイクロ流路チップ100は、マイクロ流路チップ部分の各部位の名称を示す参考正面図及び参考背面図に示すように、サンプル液インレット、シース液インレット、バッファ液インレット、分取流路の末端、及び分岐流路の末端を同一側面に有しており、前記同一側面側(チューブが挿入された側)にはチューブガードが備えられ、導入されたサンプル液及びシース液が流れる主流路の一部には光学検出領域が備えられ、回収されるべき微小粒子が通流する分取流路の一部にはアクチュエータにより加振される加振領域が備えられている。前記マイクロ流路チップでは、サンプル液収容部201と連結したサンプル液インレットから導入されたサンプル液を、シース液収容部205と連結したシース液インレットから導入されたシース液によって液送し、前記光学検出領域にて微小粒子を検出した後、回収されるべき微小粒子含む液体はアクチュエータにより加振領域に対して負圧を発生させることで分取流路へ分取され、目標サンプル貯留部203へと移送される。また、回収されるべきでない微小粒子を含む液体は、分岐流路を経て排出され、廃棄部204へと移送される。更に、バッファ液収容部206と連結しバッファ液インレットから導入されたバッファ液により形成される流れは、既に回収された微小粒子が主流路へと放出されること防ぐ。なお、前記デバイスは、板状構造体の中にダンパー207及び圧力計センサ208も備えており、これらは送液による圧力をなるべく一定にするために設けられている。This article is a disposable device used for flow cytometry to separate microparticles such as cells and microbeads. The device is used by being set on a closed-type cell sorter, and each part is hermetically connected and is used as an inseparable unit, allowing the target microscopic particles to be collected without coming into contact with the outside air. Particle separation can be performed. As shown in the reference front view showing each part, the device includes, in addition to the microchannel chip 100, a sample storage section 201, a pre-sample storage section 2011, a filter section 202, a target sample storage section 203, a waste section 204, It includes a sheath liquid storage part 205, a buffer liquid storage part 206, a damper 207, and a pressure gauge sensor 208, and these parts are connected by a tube. In this device, a liquid (sample liquid) containing microparticles introduced into a pre-sample storage section 2011 moves to the sample storage section 201 via a filter section 202, is introduced into the microchannel chip 100, and is collected. The microparticles are sorted into those that are suitable and those that are not. The microchannel chip 100 has a sample liquid inlet, a sheath liquid inlet, a buffer liquid inlet, and an end of a preparative separation channel, as shown in a reference front view and a reference rear view showing the names of each part of the microchannel chip. , and the ends of the branch channels are on the same side, and a tube guard is provided on the same side (the side where the tube is inserted), and one of the main channels through which the introduced sample liquid and sheath liquid flow. The part is equipped with an optical detection area, and the part of the separation channel through which the microparticles to be collected flow is equipped with an excitation area that is vibrated by an actuator. In the microchannel chip, the sample liquid introduced from the sample liquid inlet connected to the sample liquid storage section 201 is transported by the sheath liquid introduced from the sheath liquid inlet connected to the sheath liquid storage section 205, and After detecting microparticles in the detection region, the liquid containing the microparticles to be collected is fractionated into the preparative flow path by generating negative pressure in the vibration region using an actuator, and then transferred to the target sample storage section 203. and will be transferred. Further, liquid containing microparticles that should not be collected is discharged through the branch flow path and transferred to the waste section 204. Furthermore, the flow formed by the buffer liquid connected to the buffer liquid storage part 206 and introduced from the buffer liquid inlet prevents the already collected microparticles from being discharged into the main flow path. Note that the device also includes a damper 207 and a pressure gauge sensor 208 in the plate-like structure, and these are provided to keep the pressure caused by liquid feeding as constant as possible.

JP2019025790F 2019-11-20 2019-11-20 Sample separation device Active JP1668100S (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2019025790F JP1668100S (en) 2019-11-20 2019-11-20 Sample separation device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2019025790F JP1668100S (en) 2019-11-20 2019-11-20 Sample separation device

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JP1668100S true JP1668100S (en) 2020-09-14

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JP2019025790F Active JP1668100S (en) 2019-11-20 2019-11-20 Sample separation device

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JP (1) JP1668100S (en)

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